Open Life Sciences最新文献

{"title":"Clinical relevance of inflammatory markers in the evaluation of severity of ulcerative colitis: A retrospective study.","authors":"Tao He, Lian-Qiang Song, Xiao-Yu Weng, Peng Pan, Hui Ding, Mei-Qin Liu, Shi-Lin Qiu, Shan-Ming Sun","doi":"10.1515/biol-2025-1088","DOIUrl":"https://doi.org/10.1515/biol-2025-1088","url":null,"abstract":"<p><p>This study aimed to investigate the clinical relevance of inflammatory markers in the severity assessment of ulcerative colitis (UC). The inflammatory markers included the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and calcium ion (Ca²⁺) levels. A retrospective analysis was on 110 patients with UC and 52 patients with irritable bowel syndrome (IBS), admitted to Weifang People's Hospital between June 2019 and February 2021. UC severity was classified using the modified Mayo score and the Montreal classification system. The study assessed the predictive accuracy and correlation of these inflammatory markers with UC severity and extent. Levels of NLR, PLR, CRP, ESR, and Ca²⁺ were significantly elevated in individuals with UC compared to those with IBS. Among patients with UC, significant differences in these markers were observed across varying severity levels as defined by the modified Mayo score. However, aside from ESR, no significant differences were noted in NLR, PLR, CRP, or Ca²⁺ levels across groups defined by lesion extent. Receiver operating characteristic curve analysis indicated that NLR exhibited the highest predictive accuracy for UC, with a cut-off value of 2.603 yielding a sensitivity of 0.545, specificity of 0.288, and an area under the curve (AUC) of 0.896. The combined use of NLR, PLR, CRP, ESR, and Ca²⁺ demonstrated superior predictive performance, achieving an AUC of 0.972, sensitivity of 0.927, and specificity of 0.923 at a cut-off value of 0.455. NLR, PLR, CRP, ESR, and Ca²⁺ exhibit predictive value for UC, with NLR demonstrating the highest individual predictive performance. The combined use of these markers enhances predictive accuracy, highlighting their potential application in clinical practice for the evaluation of severity UC. Due to ethical considerations at our institution, the IBS group was used as a substitute for healthy controls. The IBS group was included solely for the calibration and testing of inflammatory biomarkers, as well as for subsequent analysis of their role in assessing UC severity.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251088"},"PeriodicalIF":1.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FTO alleviated the diabetic nephropathy progression by regulating the N6-methyladenosine levels of DACT1.","authors":"Xuanwen Li, Qing Huang, Shinong Gu, Ping Zheng","doi":"10.1515/biol-2022-1049","DOIUrl":"https://doi.org/10.1515/biol-2022-1049","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) is one of the most important microvascular complications of diabetes. The role of epigenetic regulation in DN has attracted much attention recently. This research was performed to explore the role of FTO in the DN progression. The renal tissues of DN patients were collected and the podocytes were stimulated with high glucose (HG) to establish the DN model <i>in vitro</i>. Western blot along with reverse transcription quantitative polymerase chain reaction assays was performed to analyze the mRNA as well as protein expressions. Immunohistochemistry and immunofluorescence were carried out to measure the FTO and DACT1 levels. The interaction between FTO/IGF2BP1 and DACT1 was verified by double luciferase reports and RNA-binding protein immunoprecipitation assays. FTO was declined, and DACT1 was enhanced in the HG-treated podocytes as well as renal tissues of DN patients. Overexpressed FTO declined the mRNA levels of MCP-1, IL-6, TNF-α, and the apoptosis rate of HG-treated podocytes. The N6-methyladenosine (m6A) levels, mRNA expression, and stability of FTO were depleted after FTO overexpression. DACT1 overexpression reversed the function of oe-FTO in podocytes stimulated with HG. Furthermore, IGF2BP1 knockdown declined the mRNA expression as well as the stability of FTO. In conclusion, FTO-medicated m6A modification of DACT1 was dependent on IGF2BP1 in DN progression.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221049"},"PeriodicalIF":1.7,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small cell lung cancer with small intestinal metastasis: Case report and literature review.","authors":"Hai Zeng, Ziguo Liu, Min Zhu, Tian-E Zhang","doi":"10.1515/biol-2025-1073","DOIUrl":"https://doi.org/10.1515/biol-2025-1073","url":null,"abstract":"<p><p>Patients with small cell lung cancer (SCLC) typically exhibit a poor prognosis, often receiving diagnoses at an advanced stage. Despite recent advances in immunotherapy, the median survival remains approximately one year. Gastrointestinal metastases from lung cancer, based on clinical experience, are exceedingly rare and associated with dire prognoses. This article details the diagnosis and management of an unusual case of SCLC with gastrointestinal metastasis. The patient's survival was notably prolonged compared to typical SCLC outcomes, providing significant clinical insight.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251073"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GRB14: A prognostic biomarker driving tumor progression in gastric cancer through the PI3K/AKT signaling pathway by interacting with COBLL1.","authors":"Chun-Bin Gu, Chuang Wang","doi":"10.1515/biol-2025-1084","DOIUrl":"https://doi.org/10.1515/biol-2025-1084","url":null,"abstract":"<p><p>Gastric cancer (GC) is a prevalent malignancy with a high incidence rate. Growth factor receptor-bound protein 14 (GRB14) is crucial in cell signal transduction and is associated with tumor growth, invasion, and metastasis. The aim of this study is to investigate the impact of GRB14 on GC growth and metastasis. GRB14 expression and prognosis in GC tissues were analyzed using bioinformatics. The GC cell lines, SGC-7901, MGC-803, BGC-823, and normal gastric epithelial cell line (GES-1) were used in this study. Cell viability, cycle progression, and apoptosis were assessed via CCK-8 and flow cytometry. The colony formation, transwell, and wound-healing assays were conducted to evaluate cell proliferation, invasion, and migration. Protein levels involved in the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway were analyzed by Western blot. GRB14 expression was significantly higher in GC tissues than adjacent healthy tissues, correlating with poor prognosis. GRB14 knockdown promoted apoptosis and inhibited cell growth, invasion, and migration, while its overexpression exhibited opposite effects. GRB14 directly interacted with cordon-bleu WH2 repeat protein like 1, facilitating PI3K/AKT signaling in GC cells. This study highlights GRB14's critical role in GC progression and suggests its potential as a therapeutic target.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251084"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>15-Lipoxygenase-2</i> deficiency induces foam cell formation that can be restored by salidroside through the inhibition of arachidonic acid effects.","authors":"Rong Huang, Xi Yong, Tingting Li, Huling Wen, Xing Zhou, Yichen Liao, Jun You, Chunlei Yu, Peng Xu, Yuquan Wang, Dan Wen, Tianqin Xia, Hao Yang, Yanqin Chen, Lei Xu, Xiaorong Zhong, Xianfu Li, Zhengmin Xu, Chunyang Zhou","doi":"10.1515/biol-2025-1091","DOIUrl":"https://doi.org/10.1515/biol-2025-1091","url":null,"abstract":"<p><p>15-Lipoxygenase-2 (15-Lox-2) is one of the key enzymes in arachidonic acid (AA) metabolic pathway, which belongs to the unsaturated fatty acid metabolic pathway. This pathway is involved in the foam cell transformation of macrophages during the progression of atherosclerosis (AS). The role of salidroside (SAL) in cardiovascular diseases has been extensively studied, but its impact on macrophage foam cell formation has not yet been clearly clarified. We aimed to determine the effects of <i>15-Lox-2</i> deficiency on macrophage (Ana-1 cell) foam cell formation, and those of SAL on <i>15-Lox-2</i>-deficient macrophages. <i>15-Lox-2</i>-deficient macrophages were generated using short hairpin RNA. Results indicated that 15-Lox-2 expression in the aorta of atherosclerotic patients is lower than that of the normal group. Additionally, <i>15-Lox-2</i> deficiency dramatically promoted macrophage uptake of oxidized low-density lipoprotein (ox-LDL) and increased the Cyclin D1 level while dramatically decreasing caspase3 expression. Furthermore, inflammation, complement, and TNF-α signaling pathways, along with IL1α, IL1β, IL18, and Cx3cl1, were activated in <i>15-Lox-2</i>-deficient macrophages. These changes were alleviated by SAL through inhibiting AA effects, and the effects of AA on macrophages could be inhibited by SAL. Consistently, phospholipase A2-inhibitor arachidonyl trifluoromethyl ketone (AACOCF3) restored these changes. In summary, SAL reversed the effects of <i>15-Lox-2</i> deficiency on macrophages by inhibiting excessive AA and may be a promising therapeutic potential in treating atherosclerosis resulting from <i>15-Lox-2</i> deficiency.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251091"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Ire1 gene on virulence and pathogenicity of <i>Candida albicans</i>.","authors":"Huihai Zhao, Lixia Qin, Mengyan Li, Mengyu Jiang, Mengge Cui, Hua Wang, Baohua Hou, Fukun Wang, Keran Jia","doi":"10.1515/biol-2022-1062","DOIUrl":"10.1515/biol-2022-1062","url":null,"abstract":"<p><p>With the extensive utilization of antifungal drugs, the drug resistance of <i>Candida albicans</i> is progressively intensifying, and the effect of empirical treatment for <i>C. albicans</i> infection is not evident. There is an urgent need for novel strategies and methods for the treatment of <i>C. albicans</i> infection. Our study utilized the previously constructed <i>C. albicans</i> Ire1 double gene deletion strain to explore the influence of the Ire1 on endoplasmic reticulum (ER) stress and pathogenicity of <i>C. albicans</i> through drug stress phenotype testing, biofilm and hyphomycete formation testing, and mouse systemic infection testing. The results indicate that Ire1 is involved in maintaining the integrity of the <i>C. albicans</i> cell wall and influencing the hyphal formation ability of <i>C. albicans</i>. Concurrently, the deletion of the Ire1 increased the sensitivity of <i>C. albicans</i> to the ER stress agents tunicamycin and dithiothreitol and diminished the biofilm formation ability of <i>C. albicans in vitro</i>, resulting in significant inhibition of the growth of <i>C. albicans</i>. In mouse models, the deletion of Ire1 completely nullified the virulence and pathogenicity of <i>C. albicans</i> in the tail vein infection. In conclusion, Ire1 might be a key target for the potential development of new therapeutic drugs and vaccines.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221062"},"PeriodicalIF":1.7,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of the PKR inhibitor, 2-aminopurine, on the replication of influenza A virus, and segment 8 mRNA splicing.","authors":"Dagny Lorent, Rafal Nowak, Monika Gazecka, Pawel Zmora, Elzbieta Lenartowicz Onyekaa","doi":"10.1515/biol-2025-1098","DOIUrl":"https://doi.org/10.1515/biol-2025-1098","url":null,"abstract":"<p><p>Influenza A virus (IAV) is a negative-sense, single-stranded RNA virus, whose genome consists of eight segments encoding multiple structural and non-structural proteins essential for viral replication and host immune evasion. Among these, segment 8 encodes two primary non-structural proteins  NS1, crucial for host immune suppression and virulence and NS2, involved in vRNP export - and their expression is regulated by splicing. Further studies are required to elucidate the role of PKR in IAV mRNA splicing regulation.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251098"},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of phenolic compound extraction from Tunisian squash by-products: A sustainable approach for antioxidant and antibacterial applications.","authors":"Walid Yeddes, Feten Zar Kalai, Iness Bettaieb Rebey, Majdi Hammami, Neji Tarchoun, Lilian Barros, Spyridon A Petropoulos, Hanen Falleh, Riadh Ksouri","doi":"10.1515/biol-2025-1096","DOIUrl":"https://doi.org/10.1515/biol-2025-1096","url":null,"abstract":"<p><p>The valorization of agricultural by-products is a key strategy for environmental sustainability. This study focuses on optimizing the extraction of phenolic compounds from by-products (peels, fibrous strands, and seeds) of two Tunisian squash landraces (e.g. Bejaoui and Karkoubi) using the response surface methodology to enhance their antioxidant and antibacterial properties. Ethanol concentration, extraction time, and temperature were the key parameters evaluated for their impact on phenolic compounds yield and bioactivity. High-performance liquid chromatography identified the major bioactive phenolic compounds, including vanillic acid, catechin gallate, hydroxytyrosol, and chlorogenic acid. The optimal extraction conditions for each by-product were defined as follows: Bejaoui peels (51.5% ethanol, 40.8°C, 50.5 min), fibrous strands (50.4% ethanol, 37.1°C, 36.3 min), and seeds (30% ethanol, 36.4°C, 8 min); Karkoubi peels (13.2% ethanol, 43.4°C, 47.2 min), fibrous strands (33.4% ethanol, 46.6°C, 10.8 min), and seeds (10.65% ethanol, 55.34°C, 23.16 min). The results demonstrated that optimizing extraction conditions may lead to significant enhancement of the total phenolic content and antiradical activity, with experimental values closely matching predictive models. Furthermore, the bioactive properties of these by-products, particularly their antibacterial activity, highlight their potential application as novel eco-friendly matrices for natural antioxidant and antimicrobial agents. This study underscores the importance of optimizing sustainable extraction techniques to maximize the valorization of agricultural waste, contributing to both environmental protection and the development of innovative natural products within the circular economy concept.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251096"},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Convallatoxin inhibits proliferation and angiogenesis of glioma cells via regulating JAK/STAT3 pathway.","authors":"Zhongfei Hao, Yaming Han, Yunfei Bo, Liwen Cao, Huijie Fang, Yufei Zhang, Qingbin Li","doi":"10.1515/biol-2022-1056","DOIUrl":"https://doi.org/10.1515/biol-2022-1056","url":null,"abstract":"<p><p>Gliomas can cause nerve cancer-related death, and surgical removal can be challenging. Convallatoxin functioned as anti-proliferation and anti-angiogenesis in cancer cells. However, convallatoxin's effect on glioma remains unclear. The aim of this study is to investigate the effect of convallatoxin on the proliferation and angiogenesis of glioma cells, and explore the underlying mechanism. Human glioma cell lines U251MG and A172 were treated with 12.5, 25, and 50 nM convallatoxin. Cell proliferation was investigated using the CCK-8 assay and colony formation assay. Migration and invasion were analyzed with transwell assays. Angiogenesis was evaluated using a tube formation assay. The phosphorylation of Janus kinase (JAK) and signal transducer and activator of transcription 3 (STAT3) was measured using Western blots. A xenotransplantation model of nude mice was used to investigate glioma progression. In U251MG and A172 cells, convallatoxin dose-dependently reduced cell viability and colony formation. Convallatoxin suppressed migration and invasion. Similarly, convallatoxin-treated cells had weakened angiogenesis. Convallatoxin downregulated JAK and STAT3 phosphorylation levels. Convallatoxin also inhibited glioma progression in nude mice xenotransplantation models. By inhibiting the JAK/STAT3 signaling pathway, convallatoxin inhibited proliferation, migration, invasion, and angiogenesis of glioma cells, proving to be a promising therapeutic candidate for gliomas.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20221056"},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indices of human impacts on landscapes: How do they reflect the proportions of natural habitats?","authors":"Markéta Šantrůčková, Katarína Demková, Tomáš Frantík, Jiří Dostálek","doi":"10.1515/biol-2025-1085","DOIUrl":"https://doi.org/10.1515/biol-2025-1085","url":null,"abstract":"<p><p>Human activities significantly influence landscapes, altering natural habitats and ecosystem services. This study examines the relationship between human impacts, measured by the hemeroby index and coefficient of anthropogenic impact (CAI´), and the presence of natural habitats in the Czech Republic. Using CORINE land cover data and natural habitat mapping, we analysed national and regional scales to assess the effectiveness of these indicators in reflecting environmental changes. Compared with the simple anthropogenic impact coefficient (CAI´), the hemeroby index, which accounts for both the quantity and quality of ecosystems, provides more detailed insights. At the national level, both indices had an equally close relationship with the proportion of natural habitats, but at the regional level, the results for the hemeroby index were better. Our findings indicate a strong negative correlation between human impacts and the proportion of natural habitats, emphasizing the importance of refined indicators for environmental monitoring and policy-making. The advantage of both indices is that they could be easily calculated from satellite images and/or land cover data. Therefore, they could be used worldwide.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251085"},"PeriodicalIF":1.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}