Open Life Sciences最新文献_第5页

Hysteroscopic observation of the association between endometrial polyps and endometriosis: a retrospective cohort study. 子宫内膜息肉与子宫内膜异位症的宫腔镜观察：一项回顾性队列研究。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-07-07 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1338

Hui Liu, Lianhua Tang

{"title":"Hysteroscopic observation of the association between endometrial polyps and endometriosis: a retrospective cohort study.","authors":"Hui Liu, Lianhua Tang","doi":"10.1515/biol-2025-1338","DOIUrl":"10.1515/biol-2025-1338","url":null,"abstract":"This single-centre retrospective study (100 women with endometrial polyps, mean age 41.8 ± 8.2 years) investigated the association between endometrial polyps and endometriosis. Endometriosis was confirmed in 13 % of patients. Multiple polyps were significantly more common in the endometriosis group (69.2 % vs. 42.5 %, P = 0.046). Women with endometriosis had higher CA125 levels (89.3 ± 45.2 vs. 18.6 ± 12.3 U/mL, P < 0.001) and higher rates of dysmenorrhoea (100 % vs. 35.6 %, P < 0.001). Independent predictors of endometriosis were dysmenorrhoea (aOR[95 % CI] = 8.45 [2.34-30.52], P = 0.001), CA125 > 35 U/mL (aOR[95 % CI] = 12.67 [3.82-42.01], P < 0.001), and multiple polyps (aOR[95 % CI] = 3.12 [1.08-9.01,], P = 0.036). CA125 > 35 U/mL showed an area under the curve (AUC) of 0.852 (95 % CI: 0.778-0.926), and the combined model achieved an AUC of 0.938 (95 % CI: 0.885-0.991) with a bootstrap-corrected AUC of 0.925. During a median follow-up of 17.5 months, polyp recurrence was significantly higher in women with EM (45.5 % vs. 17.6 %, P = 0.047). These findings demonstrate that the combination of multiple polyps, dysmenorrhoea, and elevated CA125 levels should raise suspicion for concurrent EM in women with EPs, suggesting potential shared pathophysiological mechanisms with important implications for clinical management, surgical planning, and fertility counselling.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251338"},"PeriodicalIF":2.3,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13340640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148405379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of eco-friendly ultrasonic probe-assisted extraction from Stevia rebaudiana Bertoni using response surface methodology and antioxidant evaluation. 响应面法及抗氧化评价优化甜菊叶的超声辅助提取工艺。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-30 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1349

Gizem Yıldırım Baştemur, Reyhan Akpinar, Emir Bora Taşçı, Beyza Pirdal, Nazlı Kılağuz, Furkan Kütükçü, Tuğsan Tenekeci, Merve Boyraz, Sabriye Percin Ozkorucuklu

{"title":"Optimization of eco-friendly ultrasonic probe-assisted extraction from Stevia rebaudiana Bertoni using response surface methodology and antioxidant evaluation.","authors":"Gizem Yıldırım Baştemur, Reyhan Akpinar, Emir Bora Taşçı, Beyza Pirdal, Nazlı Kılağuz, Furkan Kütükçü, Tuğsan Tenekeci, Merve Boyraz, Sabriye Percin Ozkorucuklu","doi":"10.1515/biol-2025-1349","DOIUrl":"10.1515/biol-2025-1349","url":null,"abstract":"In this study, an environmentally friendly ultrasonic probe-assisted extraction (UPAE) method was modeled and optimized to enhance the recovery of rebaudioside A, resveratrol, and total phenolic content (TPC) from Stevia rebaudiana Bertoni using response surface methodology. A central composite design was applied to investigate the effects of extraction time (1-9 min), ethanol concentration (0-100 %), plant amount (0.1-0.5 g), and ultrasonic frequency (20-28 kHz). Extraction efficiency was evaluated through TPC analysis and quantitative determination of rebaudioside A and resveratrol by high-performance liquid chromatography (HPLC). Green chemistry principles were integrated into extraction and analytical procedures by employing ethanol as a sustainable solvent and eliminating toxic organic modifiers. Optimal conditions were determined as 7 min extraction time, 75 % ethanol, 0.2 g plant material, and 22 kHz frequency. Under these conditions, rebaudioside A, resveratrol, and TPC were obtained as 281.59 μg/mg, 5.74 μg/mg, and 54.17 mg GAE/g, respectively, showing strong agreement with model predictions. The optimized extract exhibited high total flavonoid content and antioxidant capacity as confirmed by DPPH, FRAP, and CUPRAC assays. The proposed UPAE-HPLC approach provides a sustainable and efficient platform for the recovery of natural sweeteners and antioxidant compounds from Stevia rebaudiana, with potential applications in food, nutraceutical, and pharmaceutical industries.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251349"},"PeriodicalIF":2.3,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13313140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148353074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic response of the wild plant Mesembryanthemum forsskalii Hochst. ex Boiss to salinity stress: de novo assembly, functional annotation, and transcription factor profiling. 野生植物叶子黄的转录组学响应。前Boiss盐度胁迫：从头组装，功能注释和转录因子分析。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-29 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1342

Sumayah I Alsanie, Faten Dhawi

{"title":"Transcriptomic response of the wild plant Mesembryanthemum forsskalii Hochst. ex Boiss to salinity stress: de novo assembly, functional annotation, and transcription factor profiling.","authors":"Sumayah I Alsanie, Faten Dhawi","doi":"10.1515/biol-2025-1342","DOIUrl":"10.1515/biol-2025-1342","url":null,"abstract":"Halophytes such as Mesembryanthemum forsskalii Hochst. ex Boiss. are well-adapted to harsh environments characterized by high salinity and drought, offering both nutritional and medicinal benefits. However, the genetic and molecular basis of salinity tolerance in M. forsskalii remains largely unexplored. This study aimed to generate novel genomic resources and identify the genes, pathways, and transcriptional regulators associated with salt stress tolerance. Plant samples were treated with NaCl at 150 mM and 400 mM, resulting in transcriptomic sequencing yielding over 50 million reads for each concentration. Differential expression gene (DEG) analysis revealed 5,606 and 665 significantly upregulated and downregulated genes, respectively, in plants irrigated with 150 mM NaCl (C vs S1), while 10,730 DEGs were identified under 400 mM NaCl (C vs S2), of which 9,970 were upregulated and 760 downregulated. De novo assembly indicated that a substantial proportion of transcripts encoded novel or functionally unknown proteins. Approximately 29 % and 21 % of transcripts under 150 and 400 mM NaCl were functionally annotated in UniProt, GO, and KEGG databases. The most enriched molecular functions included manganese ion binding and oxidoreductase activity. Transcription factor families such as bHLH, NAC and MYB-related family proteins were highly represented. These findings will support salinity research.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251342"},"PeriodicalIF":2.3,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13312723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148353004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Traumatic ulcerative granuloma with stromal eosinophilia (TUGSE) in children: a case report and literature review. 儿童创伤性溃疡性肉芽肿伴间质嗜酸性粒细胞增多（TUGSE）： 1例报告并文献复习。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-26 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1328

Haihong Wang, Juanjuan Zong

{"title":"Traumatic ulcerative granuloma with stromal eosinophilia (TUGSE) in children: a case report and literature review.","authors":"Haihong Wang, Juanjuan Zong","doi":"10.1515/biol-2025-1328","DOIUrl":"10.1515/biol-2025-1328","url":null,"abstract":"Traumatic ulcerative granuloma with stromal eosinophilia (TUGSE) is a rare, self-limiting, chronic benign lesion of the oral mucosa, whose pathophysiology remains unclear. This article reports an 8-year-old male child who presented with recurrent oral ulcers for more than 2 years, with non-fixed ulcer locations. In the past year, the frequency of ulcer occurrences increased, and healing was delayed. In the past month, the pain intensified, affecting his eating. Oral examination revealed a 30 mm × 15 mm deep ulcer in the left lower posterior teeth area, covered with a yellow-white pseudomembrane and without surrounding hyperemia. Histopathology showed dense eosinophil infiltration and skeletal muscle inflammation. Immunohistochemistry demonstrated positivity for CD30, CD34 and other markers, confirming the diagnosis of TUGSE Treatment included correcting poor oral habits, topical application of growth factor gel, and symptomatic medication. Follow-up showed improvement in ulcer recurrence frequency and size, but complete control was not achieved. TUGSE in children manifests as refractory recurrences, requiring histopathological diagnosis to exclude malignancies and other diseases. Local treatment can alleviate symptoms, but long-term recurrences suggest the need for further exploration of underlying etiologies and personalized interventions.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251328"},"PeriodicalIF":2.3,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13307390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the chicken manure to corn straw ratio and assessment of bacterial diversity during composting. 鸡粪与玉米秸秆配比优化及堆肥过程中细菌多样性评价。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-26 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1306

Qiuliang Yan, Xin Liu, Xiaogang Yan, Ming Li, Zhibin Ban, Xing'ai Gao, Long Chen, Zhichao Lyu, Zhonghe Li

{"title":"Optimization of the chicken manure to corn straw ratio and assessment of bacterial diversity during composting.","authors":"Qiuliang Yan, Xin Liu, Xiaogang Yan, Ming Li, Zhibin Ban, Xing'ai Gao, Long Chen, Zhichao Lyu, Zhonghe Li","doi":"10.1515/biol-2025-1306","DOIUrl":"10.1515/biol-2025-1306","url":null,"abstract":"To enhance the quality of chicken manure and corn straw mixed compost, we evaluated the changes in physical, chemical indicators, and bacterial community diversity throughout the composting process. Three experimental groups were established with varying ratios of chicken manure to corn straw. The compost temperature was monitored daily, and samples were routinely collected to assess physical and chemical indicators. The results indicated that Group A (with a 6:4 ratio) entered the thermophilic phase earlier, sustained this phase for 20 days, exhibited faster organic matter degradation rates, and had a significantly higher total nutrient content by the end of composting compared to the other groups. The 16S rRNA high-throughput sequencing technology was utilized to investigate the alterations and distinctions in the bacterial community across 5 sampling layers of Group A samples on days 5, 15, 25 and 45. It was found that the diversity of bacterial communities initially increased, then decreased, followed by a slow increase and eventually stabilized. The predominant bacterial phyla during composting were Firmicutes, followed by Actinobacteria and Proteobacteria. The distribution ratio of bacteria in each sampling layer at each stage was small, while the main bacterial genera included Bacillus, Corynebacterium, Tepidimicrobium, Halocella, Planifilum, Luteimonas, and Actinomadura. Linear discriminant analysis revealed that the bacterial community with the greatest impact on organic matter degradation was the genus Bacillus (Firmicutes).","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251306"},"PeriodicalIF":2.3,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13307393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular effects of adamantane derivatives in P-glycoprotein leukemia cells. 金刚烷衍生物在p -糖蛋白白血病细胞中的细胞效应。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-26 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1340

Natalia Dostalkova, Lucia Sofrankova, Jana Spaldova, Branislav Pavilek, Ester Pisarova, Lakatos Boris

{"title":"Cellular effects of adamantane derivatives in P-glycoprotein leukemia cells.","authors":"Natalia Dostalkova, Lucia Sofrankova, Jana Spaldova, Branislav Pavilek, Ester Pisarova, Lakatos Boris","doi":"10.1515/biol-2025-1340","DOIUrl":"10.1515/biol-2025-1340","url":null,"abstract":"Acute myeloid leukemia is a severe hematologic malignancy that predominantly affects older adults and is characterized by high biological variability. One of the main challenges in its treatment is the development of multidrug resistance, often mediated by the overexpression of P-glycoprotein (ABCB1). This transport protein actively expels cytotoxic drugs from cells, reducing the effectiveness of chemotherapy and worsening patient prognosis. Although several generations of P-glycoprotein inhibitors have been developed, their clinical use is significantly limited due to their negative side effects. In recent years, attention has shifted toward natural substances and well-known drugs with broad applications. A promising candidate is amantadine, a lipophilic compound with a stable structure, originally used to treat influenza and Parkinson's disease. This study focuses on investigating the potential of its derivatives in combating multidrug resistance in acute myeloid leukemia. The results demonstrate that the newly synthesized adamantane derivatives, particularly E-A2, exhibit significant cytotoxic activity in acute myeloid leukemia cell lines, including P-glycoprotein-expressing resistant variants. Importantly, E-A2 was able to induce both apoptotic and necrotic cell death and maintained its activity in multidrug resistant models.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251340"},"PeriodicalIF":2.3,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13296374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism investigation of Actinidia arguta total flavone on gout via network pharmacology and vivo experiments pharmacological verification. 猕猴桃总黄酮对痛风作用机理的网络药理学研究和体内实验药理学验证。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-18 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1305

Yilin Wang, Shuang Wang, Zhaoxia Li, Xi Chen, Chengyi Zhang, Xiaoxue Han, Limei Wan, Suiqiang Fan, Yiting Liu, Ziyan Guo, Xiaoqiang Huang, Xiaoze Xu, Xin Zhou, Bingfeng Xing

{"title":"Mechanism investigation of Actinidia arguta total flavone on gout via network pharmacology and vivo experiments pharmacological verification.","authors":"Yilin Wang, Shuang Wang, Zhaoxia Li, Xi Chen, Chengyi Zhang, Xiaoxue Han, Limei Wan, Suiqiang Fan, Yiting Liu, Ziyan Guo, Xiaoqiang Huang, Xiaoze Xu, Xin Zhou, Bingfeng Xing","doi":"10.1515/biol-2025-1305","DOIUrl":"10.1515/biol-2025-1305","url":null,"abstract":"To clarify the therapeutic targets and signaling pathways of Actinidia arguta total flavone (AATF) in gout treatment, we integrated network pharmacology and in vivo experiments. Network pharmacology was applied to screen AATF's anti-gout targets, construct protein-protein interaction (PPI) and drug-component-disease-target-pathway networks, and conduct gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. A dual gout rat model was established using potassium oxonate, adenine, and monosodium urate (MSU) crystals, followed by assessments including toe swelling measurement, hematoxylin-eosin (HE) staining of kidney and joint tissues, digital radiography (DR) imaging, detection of serum uric acid and xanthine oxidase (XOD) activity, enzyme-linked immunosorbent assay (ELISA) of inflammatory factors, and Western blot validation of key targets. Network pharmacology revealed that AATF modulates inflammatory responses and the tumor necrosis factor (TNF) pathway via core targets including albumin (ALB), TNF, interleukin-6 (IL-6), and tumor protein 53 (TP53). In vivo experiments showed that AATF significantly ameliorated renal and joint pathological damage, reduced serum uric acid/XOD activity, downregulated serum interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6, cyclooxygenase-2 (COX-2) levels, and inhibited joint nuclear factor kappa B (NF-κB), extracellular signal-regulated kinase 1 (ERK1), matrix metalloproteinase 9 (MMP9) expression. Collectively, AATF exerts anti-gout effects through multi-target and multi-pathway mechanisms linked to the TNF signaling pathway, providing critical preliminary evidence for its preclinical development.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251305"},"PeriodicalIF":2.3,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13274577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Irofulven and RSL3 synergistically target ferroptosis-related gene PTGR1 to treat head and neck squamous cell carcinoma. Irofulven和RSL3协同靶向铁中毒相关基因PTGR1治疗头颈部鳞状细胞癌

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-11 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1332

Xiyuan Li, Lu Lu, Shuyao Yang, Yinglin Su, Chenfei Liu, Jingxia Xu, Lezong Chen, Chunlei Dai, Xumiao Zhang, Zhenzhen Chou, Mingyuan Du, Qiaohong Lin, Jian Meng, Shiting Zhang, Xiao Zhang, Yuanbin Song, Ming Song

{"title":"Irofulven and RSL3 synergistically target ferroptosis-related gene PTGR1 to treat head and neck squamous cell carcinoma.","authors":"Xiyuan Li, Lu Lu, Shuyao Yang, Yinglin Su, Chenfei Liu, Jingxia Xu, Lezong Chen, Chunlei Dai, Xumiao Zhang, Zhenzhen Chou, Mingyuan Du, Qiaohong Lin, Jian Meng, Shiting Zhang, Xiao Zhang, Yuanbin Song, Ming Song","doi":"10.1515/biol-2025-1332","DOIUrl":"10.1515/biol-2025-1332","url":null,"abstract":"Head and neck squamous cell carcinoma (HNSCC) is an aggressive form of cancer characterized by significant fatality rates and unfavorable clinical outcomes. Ferroptosis serves as a crucial mechanism in controlling oncogenesis and tumor growth. However, the strategy targeting ferroptosis-related gene to predict and treat HNSCC is still not clear. The study was designed to assess the potential of ferroptosis as a biomarker and therapeutic target in HNSCC. A prognostic model based on ferroptosis-related genes was constructed using least absolute shrinkage and selection operator (LASSO). Multi-omic analysis revealed that the model was closely related to DNA methylation, DNA mutation and immune infiltration. HNSCC classification and the risk score in each classification were identified by analyzing single-cell RNA-sequencing (scRNA-seq) data. The results demonstrated that the risk score in malignant tumor classification is the highest, which is relative to the progression of HNSCC. Furthermore, through CellMiner database, we found the small molecular drug irofulven could target the risk score-related gene prostaglandin reductase 1 (PTGR1), which could inhibit the ferroptosis levels in HNSCC. Importantly, irofulven and ferroptosis inducer RSL3 exerted significant synergistic effects on HNSCC in vitro and in vivo. Altogether, this study offers a potential strategy for developing a combination therapy for targeting ferroptosis in the treatment of HNSCC.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251332"},"PeriodicalIF":2.3,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13267736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148265675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triptolide alleviates rheumatoid arthritis via modulating gut microbiota - intestinal barrier - TLR4/NF-κB axis. 雷公藤甲素通过调节肠道菌群-肠道屏障- TLR4/NF-κ b轴减轻类风湿关节炎。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-08 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1290

Xuekang Pan, Zeyuan Jin, Jiaqi Bi, Huigen Lu, Gang Chen

{"title":"Triptolide alleviates rheumatoid arthritis via modulating gut microbiota - intestinal barrier - TLR4/NF-κB axis.","authors":"Xuekang Pan, Zeyuan Jin, Jiaqi Bi, Huigen Lu, Gang Chen","doi":"10.1515/biol-2025-1290","DOIUrl":"10.1515/biol-2025-1290","url":null,"abstract":"Rheumatoid arthritis (RA) is a chronic autoimmune disease driven by dysregulated inflammation and intestinal microbiota-gut-joint axis dysfunction. While triptolide, a natural immunosuppressive compound, exhibits anti-RA activity, its mechanism linking gut microbiota modulation, intestinal barrier repair, and inflammatory signaling remains unclear. This study aimed to investigate whether triptolide alleviates adjuvant-induced arthritis (AIA) in mice by regulating the gut microbiota-intestinal barrier-toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) axis. The anti-inflammatory effect of triptolide was assessed using lipopolysaccharide (LPS)-induced RAW 264.7 cells and primary peritoneal macrophages. The anti-RA efficacy was investigated in AIA mice through detecting inflammatory factors, intestinal barrier integrity and gut microbiota. The results showed that triptolide decreased inflammatory factors in both RAW 264.7 cells and primary peritoneal macrophages. Meanwhile, triptolide dose-dependently reduced AI scores and paw swelling in AIA mice (p < 0.05 vs. AIA group), along with restored intestinal barrier integrity by upregulating ZO-1 and Occludin (p < 0.05 vs. AIA group) and modulated gut microbiota. Mechanistically, triptolide inhibited TLR4/NF-κB activation, reducing downstream pro-inflammatory cytokines (interleukin-6 (IL-6), tumor necrosis factor α (TNF-α)). In sum, these findings reveal that triptolide alleviates AIA by modulating the gut microbiota, repairing the intestinal barrier, and suppressing TLR4/NF-κB signaling. This highlights a novel \"microbiota-gut-joint\" regulatory mechanism of triptolide, supporting its potential as a therapeutic agent targeting RA's multifactorial pathogenesis.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251290"},"PeriodicalIF":2.3,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148205746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling molecular mechanisms and therapeutic targets in HbH-CS disease: a focus on oxidative stress and mitochondrial dysfunction. 揭示HbH-CS疾病的分子机制和治疗靶点：关注氧化应激和线粒体功能障碍。

IF 2.3 4区生物学

Open Life Sciences Pub Date : 2026-06-05 eCollection Date: 2026-01-01 DOI: 10.1515/biol-2025-1337

Liuying Nong, Ling Shi, Lihong Pang

{"title":"Unveiling molecular mechanisms and therapeutic targets in HbH-CS disease: a focus on oxidative stress and mitochondrial dysfunction.","authors":"Liuying Nong, Ling Shi, Lihong Pang","doi":"10.1515/biol-2025-1337","DOIUrl":"10.1515/biol-2025-1337","url":null,"abstract":"This study aimed to investigate the molecular mechanisms and therapeutic targets related to oxidative stress and mitochondrial dysfunction in Hemoglobin H-Constant Spring (HbH-CS) disease. HbH-CS differentially expressed genes (DEGs) were selected from a microarray dataset. Oxidative stress-related genes (OSGs) and mitochondrial function-related genes (MiRGs) were retrieved from public databases. Oxidative stress and mitochondrial function-related genes (OMRGs) were defined as the intersection of HbH-CS DEGs, OSGs, and MiRGs. Programmed cell death (PCD) mechanisms and functional enrichment analyses were subsequently investigated. A protein-protein interaction (PPI) network was constructed to identify hub genes, and potential regulatory mechanisms and expression levels of candidate genes were further explored. A total of 98 OMRGs were identified, which were associated with cellular respiration, oxidative stress response, mitochondrial membrane, and apoptotic signaling. Apoptosis was determined to be the primary PCD mechanism. Three hub genes were identified: AKT serine/threonine kinase 1 (AKT1), B cell lymphoma/leukemia-2 (BCL2), and cytochrome c, somatic (CYCS). Additionally, RNA-binding motif protein 15B (RBM15B) was recognized as a shared N6-methyladenosine (m6A) regulator. The mRNA expression levels of these four genes were significantly downregulated in HbH-CS patients. These findings provide novel insights into the molecular mechanisms and identify potential therapeutic targets for clinical intervention.","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"21 1","pages":"20251337"},"PeriodicalIF":2.3,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13238148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0