贵州省CDI患者粪便中分离的一株新的ST11(PR34365)艰难梭菌的基因组分析

IF 1.7 4区 生物学 Q3 BIOLOGY
Open Life Sciences Pub Date : 2025-05-20 eCollection Date: 2025-01-01 DOI:10.1515/biol-2025-1067
Ying Yang, Luhong Shu, Ping Ling, Junyi Yang, Ruirui Shao, Yumei Cheng, Shanshan Luo, Xinglang Wei, Zhizhong Guan, Zhenghong Chen, Jian Liao, Xiaolan Qi, Guzhen Cui, Wei Hong
{"title":"贵州省CDI患者粪便中分离的一株新的ST11(PR34365)艰难梭菌的基因组分析","authors":"Ying Yang, Luhong Shu, Ping Ling, Junyi Yang, Ruirui Shao, Yumei Cheng, Shanshan Luo, Xinglang Wei, Zhizhong Guan, Zhenghong Chen, Jian Liao, Xiaolan Qi, Guzhen Cui, Wei Hong","doi":"10.1515/biol-2025-1067","DOIUrl":null,"url":null,"abstract":"<p><p><i>Clostridioides difficile</i> is a pathogen that causes pseudomembranous colitis with antibiotic-associated diarrhea. The epidemiology and molecular evolution of <i>C. difficile</i> may differ among different geographic regions, and mining its genomic information can help to understand the epidemiology and molecular evolution of <i>C. difficile</i> and focus on its transmission mode. A <i>C. difficile</i> strain denoted WXL8 was isolated from a human fecal sample from a patient in the intensive care unit, and its physiology and genomic sequence were determined. The total genome size of WXL8 was 4,119,929 bp, and the GC content was 27.97%. The multilocus sequence typing results indicated that WXL8 is strain type 11 (ST11), a genotype widely present in livestock. The WXL8 was located in clade 5 of ST11. The ribotype of WXL8 was a novel ribotype (PR34365). It is the first report of the ST11 (PR34365) strain. Comparative genomic analysis between WXL8 and the other four high-virulence strains (CD630, CDBR81, CDS-0253, and CDR20291) showed differences in gene arrangement, indicating the uniqueness of <i>C. difficile</i> WXL8. In the present study, a novel ribotype (PR34365) ST11 strain of <i>C. difficile</i> was isolated from a patient with diarrhea in Guizhou, China. Our findings suggest that zoonotic CDI should receive more clinical attention.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"20 1","pages":"20251067"},"PeriodicalIF":1.7000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103185/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genomic analysis of a novel ST11(PR34365) <i>Clostridioides difficile</i> strain isolated from the human fecal of a CDI patient in Guizhou, China.\",\"authors\":\"Ying Yang, Luhong Shu, Ping Ling, Junyi Yang, Ruirui Shao, Yumei Cheng, Shanshan Luo, Xinglang Wei, Zhizhong Guan, Zhenghong Chen, Jian Liao, Xiaolan Qi, Guzhen Cui, Wei Hong\",\"doi\":\"10.1515/biol-2025-1067\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Clostridioides difficile</i> is a pathogen that causes pseudomembranous colitis with antibiotic-associated diarrhea. The epidemiology and molecular evolution of <i>C. difficile</i> may differ among different geographic regions, and mining its genomic information can help to understand the epidemiology and molecular evolution of <i>C. difficile</i> and focus on its transmission mode. A <i>C. difficile</i> strain denoted WXL8 was isolated from a human fecal sample from a patient in the intensive care unit, and its physiology and genomic sequence were determined. The total genome size of WXL8 was 4,119,929 bp, and the GC content was 27.97%. The multilocus sequence typing results indicated that WXL8 is strain type 11 (ST11), a genotype widely present in livestock. The WXL8 was located in clade 5 of ST11. The ribotype of WXL8 was a novel ribotype (PR34365). It is the first report of the ST11 (PR34365) strain. Comparative genomic analysis between WXL8 and the other four high-virulence strains (CD630, CDBR81, CDS-0253, and CDR20291) showed differences in gene arrangement, indicating the uniqueness of <i>C. difficile</i> WXL8. In the present study, a novel ribotype (PR34365) ST11 strain of <i>C. difficile</i> was isolated from a patient with diarrhea in Guizhou, China. Our findings suggest that zoonotic CDI should receive more clinical attention.</p>\",\"PeriodicalId\":19605,\"journal\":{\"name\":\"Open Life Sciences\",\"volume\":\"20 1\",\"pages\":\"20251067\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103185/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Life Sciences\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1515/biol-2025-1067\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Life Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1515/biol-2025-1067","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

艰难梭菌是一种病原体,可引起假膜性结肠炎伴抗生素相关性腹泻。艰难梭菌的流行病学和分子进化在不同的地理区域可能存在差异,挖掘其基因组信息有助于了解艰难梭菌的流行病学和分子进化,并关注其传播模式。从重症监护病房患者的粪便样本中分离出一株艰难梭菌菌株WXL8,并测定了其生理和基因组序列。WXL8的总基因组大小为4,119,929 bp, GC含量为27.97%。多位点序列分型结果表明,WXL8为11型菌株(ST11),该基因型广泛存在于家畜中。WXL8位于ST11的分支5。WXL8的核糖型为新型核糖型(PR34365)。这是首次报道的ST11 (PR34365)毒株。WXL8与其他4株高毒力菌株(CD630、CDBR81、CDS-0253和CDR20291)的基因组比较分析显示,基因排列存在差异,说明难辨梭菌WXL8具有独特性。在本研究中,从中国贵州的一名腹泻患者中分离到一株新的难辨梭菌(PR34365) ST11菌株。我们的研究结果提示人畜共患CDI应得到更多的临床关注。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genomic analysis of a novel ST11(PR34365) Clostridioides difficile strain isolated from the human fecal of a CDI patient in Guizhou, China.

Clostridioides difficile is a pathogen that causes pseudomembranous colitis with antibiotic-associated diarrhea. The epidemiology and molecular evolution of C. difficile may differ among different geographic regions, and mining its genomic information can help to understand the epidemiology and molecular evolution of C. difficile and focus on its transmission mode. A C. difficile strain denoted WXL8 was isolated from a human fecal sample from a patient in the intensive care unit, and its physiology and genomic sequence were determined. The total genome size of WXL8 was 4,119,929 bp, and the GC content was 27.97%. The multilocus sequence typing results indicated that WXL8 is strain type 11 (ST11), a genotype widely present in livestock. The WXL8 was located in clade 5 of ST11. The ribotype of WXL8 was a novel ribotype (PR34365). It is the first report of the ST11 (PR34365) strain. Comparative genomic analysis between WXL8 and the other four high-virulence strains (CD630, CDBR81, CDS-0253, and CDR20291) showed differences in gene arrangement, indicating the uniqueness of C. difficile WXL8. In the present study, a novel ribotype (PR34365) ST11 strain of C. difficile was isolated from a patient with diarrhea in Guizhou, China. Our findings suggest that zoonotic CDI should receive more clinical attention.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.50
自引率
4.50%
发文量
131
审稿时长
43 weeks
期刊介绍: Open Life Sciences (previously Central European Journal of Biology) is a fast growing peer-reviewed journal, devoted to scholarly research in all areas of life sciences, such as molecular biology, plant science, biotechnology, cell biology, biochemistry, biophysics, microbiology and virology, ecology, differentiation and development, genetics and many others. Open Life Sciences assures top quality of published data through critical peer review and editorial involvement throughout the whole publication process. Thanks to the Open Access model of publishing, it also offers unrestricted access to published articles for all users.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信