Liupao tea aqueous extract alleviates dextran sulfate sodium-induced ulcerative colitis in rats by modulating the gut microbiota.

IF 1.7 4区 生物学 Q3 BIOLOGY
Open Life Sciences Pub Date : 2025-05-20 eCollection Date: 2025-01-01 DOI:10.1515/biol-2025-1106
Shengjin Ming, Jinxi Ou, Ying Huang, Zhongqing Tang, Yuechao Qin, Hongxi Ma, Siling Gan, Zhongxia Li
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Abstract

Liupao tea is known for its anti-inflammatory antioxidant and regulation of gut microecological balance properties. This study aims to investigate the therapeutic effects of Liupao tea aqueous extract (LPTAE) on ulcerative colitis (UC) induced by dextran sulfate sodium (DSS) in rats. The rats were randomly divided into five groups: the Normal group, the DSS group, the LPTL group, the LPTM group, and the LPTH group. Throughout the experiment, the rats' activity levels, stool consistency, and body weights were observed and recorded daily. After the experiment, colon length was measured, and colon tissues were collected for pathological analysis. Additionally, the colon contents were analyzed for gut microbiota composition and short-chain fatty acid (SCFA) level, while serum samples were collected to determine inflammatory and oxidative factors. The results indicated that treatment with low, medium, and high doses of LPTAE significantly inhibited weight loss, alleviated rectal bleeding, and reduced colon shortening compared to the DSS group. It also decreased the disease activity index and histopathological activity index scores in the rats. Furthermore, LPTAE reduced the levels of inflammatory cytokines such as IL-1β, IL-6, TNF-α, and malondialdehyde, while simultaneously increasing the levels of superoxide dismutase and SCFAs, including acetic acid, propionic acid, and butyric acid. 16S rDNA gene sequencing of the gut microbiota revealed that all doses of LPTAE reversed the decrease in both α and β diversities caused by UC, increased the relative abundance of beneficial bacteria such as Lactobacillus, Muribaculaceae, Alloprevotella, and Blautia, and decreased the levels of harmful bacteria such as Prevotella, Romboutsia, and Bacteroides. In summary, within the tested doses (100, 150, 250 mg/kg), LPTAE alleviated DSS-induced colitis by modulating the gut microbiota and correcting the metabolic imbalance of SCFAs.

六保茶水提物通过调节肠道菌群减轻硫酸葡聚糖钠诱导的大鼠溃疡性结肠炎。
六保茶以其抗炎、抗氧化和调节肠道微生态平衡的特性而闻名。本研究旨在探讨六保茶水提物(LPTAE)对硫酸葡聚糖钠(DSS)所致大鼠溃疡性结肠炎(UC)的治疗作用。将大鼠随机分为5组:正常组、DSS组、LPTL组、LPTM组、LPTH组。在整个实验过程中,每天观察并记录大鼠的活动水平、粪便稠度和体重。实验结束后测量结肠长度,收集结肠组织进行病理分析。此外,分析结肠内容物的肠道微生物群组成和短链脂肪酸(SCFA)水平,并收集血清样本以检测炎症和氧化因子。结果显示,与DSS组相比,低、中、高剂量LPTAE治疗显著抑制体重减轻,减轻直肠出血,减少结肠缩短。它还降低了大鼠的疾病活动指数和组织病理学活动指数得分。此外,LPTAE降低了炎症细胞因子如IL-1β、IL-6、TNF-α和丙二醛的水平,同时增加了超氧化物歧化酶和scfa的水平,包括乙酸、丙酸和丁酸。肠道菌群16S rDNA基因测序结果显示,所有剂量的LPTAE均逆转了UC引起的α和β多样性的下降,增加了有益细菌如乳酸杆菌、Muribaculaceae、Alloprevotella和Blautia的相对丰度,降低了有害细菌如Prevotella、Romboutsia和Bacteroides的水平。综上所述,在测试剂量(100、150、250 mg/kg)内,LPTAE通过调节肠道微生物群和纠正scfa的代谢失衡来减轻dss诱导的结肠炎。
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来源期刊
CiteScore
2.50
自引率
4.50%
发文量
131
审稿时长
43 weeks
期刊介绍: Open Life Sciences (previously Central European Journal of Biology) is a fast growing peer-reviewed journal, devoted to scholarly research in all areas of life sciences, such as molecular biology, plant science, biotechnology, cell biology, biochemistry, biophysics, microbiology and virology, ecology, differentiation and development, genetics and many others. Open Life Sciences assures top quality of published data through critical peer review and editorial involvement throughout the whole publication process. Thanks to the Open Access model of publishing, it also offers unrestricted access to published articles for all users.
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