npj Biofilms and Microbiomes最新文献_第9页

Multikingdom microbiome-based machine learning enables multiple sclerosis diagnosis. 基于多领域微生物组的机器学习使多发性硬化症诊断成为可能。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-30 DOI: 10.1038/s41522-026-01030-7

Gaochen Zhu, Guan Yang

{"title":"Multikingdom microbiome-based machine learning enables multiple sclerosis diagnosis.","authors":"Gaochen Zhu, Guan Yang","doi":"10.1038/s41522-026-01030-7","DOIUrl":"10.1038/s41522-026-01030-7","url":null,"abstract":"Emerging evidence suggests a role for the gut bacteria in the pathogenesis of multiple sclerosis (MS); however, the role of other microorganisms and their diagnostic potential for MS remain poorly explored. Here, we analyzed large-scale metagenomic data derived from fecal samples (discovery cohort n = 1152; total n = 1306 across 3 geographically diverse cohorts). Subsequently, we utilized multikingdom gut microbiome data to develop machine learning models to distinguish MS patients from healthy controls. Our analysis identified distinct microbiome alterations, revealing 90 bacterial, 3 fungal, 2 viral species, 119 KEGG orthology genes, and 17 metabolic pathways significantly associated with MS. Machine learning models integrating multikingdom taxonomic and functional features achieved the area under the receiver operating characteristic curves (AUCs) of 0.977 for males and 0.978 for females. On external validation datasets, the ensemble models yielded AUCs of 0.813 in males and 0.745 in females, while the 30-marker models reached AUCs of 0.849 and 0.763, respectively. Notably, the accuracy of the model was associated with Faecalibacterium spp. and L-methionine biosynthesis pathways, which were less abundant in MS patients. Collectively, our findings highlight the potential application of multikingdom and functional gut microbiome markers as non-invasive biomarkers for MS.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13519030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148101578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined high-quality metagenomics reveals off-target effects of albendazole, ivermectin-albendazole and moxidectin-albendazole on the human gut bacteria. 联合高质量宏基因组学揭示了阿苯达唑、伊维菌素-阿苯达唑和莫西菌素-阿苯达唑对人肠道细菌的脱靶效应。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-29 DOI: 10.1038/s41522-026-01018-3

Julian Dommann, Viviane P Sprecher, Christian Beisel, Daniel Ballmer, Eveline Hürlimann, Jean T Coulibaly, Jennifer Keiser, Pierre H H Schneeberger

{"title":"Combined high-quality metagenomics reveals off-target effects of albendazole, ivermectin-albendazole and moxidectin-albendazole on the human gut bacteria.","authors":"Julian Dommann, Viviane P Sprecher, Christian Beisel, Daniel Ballmer, Eveline Hürlimann, Jean T Coulibaly, Jennifer Keiser, Pierre H H Schneeberger","doi":"10.1038/s41522-026-01018-3","DOIUrl":"10.1038/s41522-026-01018-3","url":null,"abstract":"Human whipworm infections caused by Trichuris trichiura and Trichuris incognita remain a major public health problem, affecting over 400 million people globally and responding poorly to standard benzimidazole chemotherapy. Ivermectin-albendazole and moxidectin-albendazole have emerged as promising combination therapies, but recent in vitro evidence suggests that ivermectin and moxidectin may also affect gut bacteria. We therefore characterized their off-target effects on the gut microbiome in a randomized controlled trial including 204 Trichuris spp.-infected individuals in Côte d'Ivoire treated with albendazole (400 mg), ivermectin-albendazole (200 µg/kg/400 mg), or moxidectin-albendazole (8 mg/400 mg). By combining Illumina short reads and Nanopore long reads, we recovered over 800 high-quality metagenome-assembled genomes. Albendazole and moxidectin-albendazole induced taxonomic shifts with only mild functional consequences. In contrast, individuals receiving higher absolute ivermectin doses based on their bodyweight ( ≥ 15 mg) showed pronounced changes in taxonomic composition and microbial function, whereas the resistome remained largely stable. These findings confirm that ivermectin can exert antibacterial off-target effects in the human gut beyond those previously observed in vitro. Given its central role in parasite control, its broader microbiome effects warrant careful evaluation in future treatment strategies.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13503709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148056011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The gut commensal Bacteroides thetaiotaomicron harbors prevalent class A and D β-lactamases with cross-taxa dissemination potential. 肠道共生拟杆菌（Bacteroides thetaiotaommicron）含有普遍存在的A类和D类β-内酰胺酶，具有跨类群传播潜力。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-28 DOI: 10.1038/s41522-026-01024-5

Tao Song, Zhangqun Hou, Chuan Zhang, Tong Luo, Peiting Wen, Ling Mei, Dezhi Yuan, Xue Shen, Hongbo Dong, Kelei Zhao, Yiwen Chu, Xinrong Wang, Fengzheng Chen, Wenxia Sun, Jiafu Lin, Wenjun Jiang

{"title":"The gut commensal Bacteroides thetaiotaomicron harbors prevalent class A and D β-lactamases with cross-taxa dissemination potential.","authors":"Tao Song, Zhangqun Hou, Chuan Zhang, Tong Luo, Peiting Wen, Ling Mei, Dezhi Yuan, Xue Shen, Hongbo Dong, Kelei Zhao, Yiwen Chu, Xinrong Wang, Fengzheng Chen, Wenxia Sun, Jiafu Lin, Wenjun Jiang","doi":"10.1038/s41522-026-01024-5","DOIUrl":"10.1038/s41522-026-01024-5","url":null,"abstract":"The abundant gut commensal Bacteroides thetaiotaomicron is repeatedly challenged by β-lactam exposure in the human intestine, yet its β-lactamase repertoire and dissemination potential remain incompletely characterized. Here, we screened 626 publicly available B. thetaiotaomicron genomes and identified 1059 putative β-lactamase homologs, all belonging to class A or class D families. Four highly prevalent representatives-BTA-1 and CfxA-3 (class A) and OXA-347 and OXA-1327 (class D)-were prioritized for experimental validation. Heterologous expression in Escherichia coli revealed heterogeneous resistance phenotypes, with ones conferring only modest MIC increases (e.g., OXA-347, ~2-fold for amoxicillin) and others producing strong resistance to penicillins (e.g., BTA-1caused a 256-fold increase in amoxicillin MIC). Purified BTA-1 and OXA-347 were active under gut-relevant conditions, with pH optima at 7 and 8 and temperature optima at 40 °C and 30 °C, respectively. Notably, OXA-347 hydrolyzed representatives of penicillins, cephalosporins, carbapenems, and monobactams, and mass spectrometry confirmed β-lactam ring opening. Beyond B. thetaiotaomicron, these β-lactamase alleles were detected across multiple gut commensal genera as well as taxa annotated as opportunistic pathogens. Genomic context analyses showed similar gene-cluster patterns in B. thetaiotaomicron, across gut commensal genera, and in opportunistic-pathogen-associated taxa. Collectively, our findings clarify the diversity, activity, and distribution of B. thetaiotaomicron-associated β-lactamases across the gut microbiome and clinically relevant taxa.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13500504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148055997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heritable gut microbiota decreases fat accumulation in pregnant sows and optimizes gestational outcomes. 可遗传的肠道微生物群减少妊娠母猪的脂肪积累，并优化妊娠结局。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-27 DOI: 10.1038/s41522-026-00998-6

Mingyu Wang, Lin Zhang, Songbai Li, Erqi Cha, Ao Guo, Boyuan Sun, Xinjian Li, Shengru Wu, Taiyong Yu

{"title":"Heritable gut microbiota decreases fat accumulation in pregnant sows and optimizes gestational outcomes.","authors":"Mingyu Wang, Lin Zhang, Songbai Li, Erqi Cha, Ao Guo, Boyuan Sun, Xinjian Li, Shengru Wu, Taiyong Yu","doi":"10.1038/s41522-026-00998-6","DOIUrl":"10.1038/s41522-026-00998-6","url":null,"abstract":"This study investigates the interplay between host genetics and gut microbiota in regulating fat deposition and reproductive outcomes in sows. Integrating multiomics data from 348 Yorkshire sows-including whole-genome sequencing, 16S rRNA sequencing, and metabolomic profiling-we identified 37 microbial taxa significantly associated with thick backfat (TBP) through microbiome-wide association studies (MWAS). Microbial genome-wide association analysis (mGWAS) revealed heritable genera, including Ezakiella and Corynebacterium, and implicated host genes such as MED17 and VSTM5 in microbial modulation. Concurrently, genome-wide association studies (GWAS) identified MC4R and MEDAG as candidate genes directly influencing TBP. Metabolomic analysis highlighted acetate, a short-chain fatty acid, as a key mediator in host adipogenesis. Functional validation in experimental models demonstrated that exogenous acetate supplementation alters lipid metabolism and enhances reproductive performance. These findings elucidate a mechanistic axis wherein host genetics shape gut microbial composition, which in turn modulates fat metabolism via acetate production, ultimately affecting pregnancy outcomes. This integrated approach provides insights into host-microbe co-regulation of metabolic traits and highlights potential targets for improving reproductive efficiency in livestock.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13578478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148043021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial inoculation drives microbiome-mediated resistance to a soil-borne pathogen in wheat. 细菌接种驱动微生物组介导的小麦对土壤传播病原体的抗性。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-26 DOI: 10.1038/s41522-026-01021-8

Caroline Sayuri Nishisaka, Hélio Danilo Quevedo, Thierry Alexandre Pellegrinetti, Fernanda de Almeida Godoy, Maike Rossmann, Lucas William Mendes, Rodrigo Mendes

{"title":"Bacterial inoculation drives microbiome-mediated resistance to a soil-borne pathogen in wheat.","authors":"Caroline Sayuri Nishisaka, Hélio Danilo Quevedo, Thierry Alexandre Pellegrinetti, Fernanda de Almeida Godoy, Maike Rossmann, Lucas William Mendes, Rodrigo Mendes","doi":"10.1038/s41522-026-01021-8","DOIUrl":"10.1038/s41522-026-01021-8","url":null,"abstract":"Soil microbiomes are fundamental to plant health, mediating nutrient cycling, stress tolerance, and pathogen defense. However, soil-borne pathogens such as Bipolaris sorokiniana severely constrain wheat productivity. Despite growing interest, the mechanisms by which beneficial bacterial inoculation reshapes rhizosphere microbial communities to enhance disease resistance remain poorly understood. Here, we isolated three bacterial strains, Streptomyces virginiae CMAA1738, Paenibacillus ottowii CMAA1739, and Pseudomonas inefficax CMAA1741, with antagonistic activity against B. sorokiniana, and evaluated their effects on wheat under controlled conditions. Through plant bioassays, bacterial inoculation reduced disease severity by ~60% and promoted root growth. Metataxonomic and metagenomic analyses revealed shifts in the structure and functional potential of the rhizosphere microbiome. Structural equation modeling indicated that inoculation was the primary driver of microbiome restructuring and disease suppression. Notably, inoculation restored the diversity of plant growth-promoting genes and biosynthetic gene clusters reduced by pathogen infection, enriching functions associated with stress tolerance, nutrient metabolism, and secondary metabolite production. In addition, Random Forest analysis revealed that variation in disease severity under pathogen pressure was associated with differences in bacterial community composition. Together, these findings demonstrate that bacterial inoculation can restructure the rhizosphere microbiome and restore key functional traits linked to plant resilience.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13482813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative analysis of oral microbiota and its gut transmission with host immunity in term pregnancy. 足月妊娠期口腔微生物群及其肠道传播与宿主免疫的综合分析。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-26 DOI: 10.1038/s41522-026-01009-4

Ting Huang, Xiaomei Tang, Huijuan Luo, Han Bao, Haishan Xie, Lulu Meng, Li Zhang, Guocheng Liu, Xiaomin Xiao

{"title":"Integrative analysis of oral microbiota and its gut transmission with host immunity in term pregnancy.","authors":"Ting Huang, Xiaomei Tang, Huijuan Luo, Han Bao, Haishan Xie, Lulu Meng, Li Zhang, Guocheng Liu, Xiaomin Xiao","doi":"10.1038/s41522-026-01009-4","DOIUrl":"10.1038/s41522-026-01009-4","url":null,"abstract":"This study aims to evaluate associations between the oral microbiota, metabolites, potential oral-gut transmitted microbes, and systemic immune alterations in term pregnancy. Oral and gut microbiomes, salivary metabolome, peripheral immune cells, and cytokines were profiled in term pregnant (n = 25) and non-pregnant women (n = 25). Species enriched in pregnancy, including Streptococcus anginosus and Prevotella denticola, correlated positively with the NK cell ratio, while Prevotella histicola and Prevotella micans correlated positively with CD56brightCD16- NK cells. Mediation analysis indicated that fatty acids in saliva may not mediate the impact of oral microbiota on peripheral immune changes during pregnancy. Several potential oral-gut transmitted microbes with a higher maternal transfer ratio were positively associated with CD56brightCD16- NK cells and the NK cell ratio, but negatively with Th1 cells and the Th1/Th2 ratio. These findings provide preliminary evidence that pregnancy-related immune shifts may be linked to oral microbiota alterations and microbial transmission.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13487304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148033638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intravesical mycobacteria reshape bladder and gut microbiota in a murine bladder cancer model. 膀胱内分枝杆菌在小鼠膀胱癌模型中重塑膀胱和肠道微生物群。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-25 DOI: 10.1038/s41522-026-01002-x

Víctor Campo-Pérez, Sandra Guallar-Garrido, Raúl Cabrera-Rubio, Paula Herrero-Abadía, Arnau Seguí-Moll, Manuela Torres-Puente, Pablo Cano-Jimenez, Iñaki Comas, Eduard Torrents, Esther Julián

{"title":"Intravesical mycobacteria reshape bladder and gut microbiota in a murine bladder cancer model.","authors":"Víctor Campo-Pérez, Sandra Guallar-Garrido, Raúl Cabrera-Rubio, Paula Herrero-Abadía, Arnau Seguí-Moll, Manuela Torres-Puente, Pablo Cano-Jimenez, Iñaki Comas, Eduard Torrents, Esther Julián","doi":"10.1038/s41522-026-01002-x","DOIUrl":"10.1038/s41522-026-01002-x","url":null,"abstract":"The bladder microbiota may influence tumor progression and responses to intravesical therapies, yet the effects of Mycobacterium bovis BCG instillations on bladder and gut microbiota remain unexplored. This study evaluates, for the first time, the impact of M. bovis BCG and Mycobacterium brumae, a non-pathogenic immunomodulator, on microbiota composition in bladder tissue and cecal stool of healthy and tumor-bearing mice. Mycobacterial instillations increased gut microbial richness but reduced Actinobacteria. In tumor-bearing mice, Muribaculaceae and Bacteroidaceae were enriched, while M. bovis BCG reduced Muribaculaceae and increased Rikenellaceae. In the bladder, M. brumae instillations favored Staphylococcaceae in healthy mice, while Enterococcaceae dominated most groups except M. bovis BCG-treated tumor-bearing mice. Escherichia/Shigella decreased in mycobacteria-treated tumor-bearing mice, while Burkholderia was more prevalent in untreated bladders. Notably, M. brumae increased stool alpha diversity, whereas M. bovis BCG reduced bladder richness. These findings reveal that intravesical mycobacterial therapy modulates microbiota composition across body sites, underscoring the complex host-tumor-immunotherapy interplay.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13482592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research advances in the identification of gut keystone bacteria and formulation of suitable dietary intervention strategies for restoring gut homeostasis. 肠道关键菌的鉴定及制定适当的饮食干预策略以恢复肠道稳态的研究进展。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-25 DOI: 10.1038/s41522-026-01020-9

Qiang Meng, Huimin Zhang, Jianming Ye, Ting Liu, Yihua Li, Wei Song, Yane Luo, Tianli Yue

引用次数: 0

Cutibacterium acnes inhibits Staphylococcus lugdunensis biofilm formation through inhibition of autolysis and purine biosynthesis. 痤疮角质杆菌通过抑制自溶和嘌呤生物合成来抑制lugdunensis的生物膜形成。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-25 DOI: 10.1038/s41522-026-01014-7

Rayssa D Lima, Olivia R Bauer, Heidi Pauer, Kiana Hajiarbabi, Daniel Andrade Moreira, Thiago Estevam Parente, Rosana B R Ferreira

{"title":"Cutibacterium acnes inhibits Staphylococcus lugdunensis biofilm formation through inhibition of autolysis and purine biosynthesis.","authors":"Rayssa D Lima, Olivia R Bauer, Heidi Pauer, Kiana Hajiarbabi, Daniel Andrade Moreira, Thiago Estevam Parente, Rosana B R Ferreira","doi":"10.1038/s41522-026-01014-7","DOIUrl":"10.1038/s41522-026-01014-7","url":null,"abstract":"Cutibacterium acnes is a key member of the human skin microbiome that contributes to host homeostasis. Staphylococcus lugdunensis, while also a resident of the skin microbiota, is an opportunistic pathogen capable of causing severe infections, associated with its ability to form biofilms. We previously showed that C. acnes secretes molecules that inhibit S. lugdunensis biofilm formation without affecting planktonic growth. Here, we demonstrate that C. acnes-derived molecules also significantly reduced S. lugdunensis adherence to and invasion of human epithelial cells, as well as adhesion to keratinocytes. Transcriptomic analysis revealed repression of genes involved in S. lugdunensis purine biosynthesis and induction of the autolysis negative regulators, lrgA and lrgB. Functional assays confirmed that exposure to C. acnes molecules inhibits autolysis and extracellular DNA (eDNA) release and decreases intracellular guanine levels in S. lugdunensis. Crucially, the addition of exogenous guanine suppressed the effect of C. acnes molecules on both biofilm formation and lrgA gene expression. Collectively, our data indicate that C. acnes molecules inhibit S. lugdunensis biofilm formation by depleting the intracellular guanine pool, leading to repression of autolysis, and reduced eDNA release, a key component of biofilm structural integrity. These findings underscore the importance of interspecies microbiome interactions in pathogen exclusion.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-9394b from Lactobacillus paracasei extracellular vesicles targets iNOS to reprogram host NO-ornithine metabolism and ameliorate DSS-induced murine colitis. 来自副干酪乳杆菌细胞外囊泡的miR-9394b靶向iNOS重编程宿主no -鸟氨酸代谢并改善dss诱导的小鼠结肠炎。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-25 DOI: 10.1038/s41522-026-01023-6

Honghao Ding, Heng Zhang, Diying Li, Zhiqi Hu, Guoqi Xiang, Qingqing Nie, Qibing Liu, Yunxiang Liang, Shuai Wang, Shiyu Tao, Yingjun Li

{"title":"miR-9394b from Lactobacillus paracasei extracellular vesicles targets iNOS to reprogram host NO-ornithine metabolism and ameliorate DSS-induced murine colitis.","authors":"Honghao Ding, Heng Zhang, Diying Li, Zhiqi Hu, Guoqi Xiang, Qingqing Nie, Qibing Liu, Yunxiang Liang, Shuai Wang, Shiyu Tao, Yingjun Li","doi":"10.1038/s41522-026-01023-6","DOIUrl":"10.1038/s41522-026-01023-6","url":null,"abstract":"Ulcerative colitis (UC) is a growing global health burden, creating an urgent need for new therapies. While extracellular vesicles (EVs) from probiotic bacteria show therapeutic potential, their molecular mechanisms are largely unknown. This study reveals that Lactobacillus paracasei (LPC) and its EVs (LPC-EVs) alleviate Dextran Sulfate Sodium Salt (DSS)-induced colitis in mice through a multi-faceted mechanism involving partial reshaping of the gut microbiota, suppression of TLR4/NF-κB signaling, and reinforcement of tight junctions. We found that miR-9394b carried by LPC-EVs plays a central role in this effect. In MODE-K cells, miR-9394b directly targets iNOS, shifting arginine metabolism away from nitric oxide (NO) production toward ornithine-associated metabolism. The increased ornithine served as a downstream mediator linking epithelial metabolic remodeling to macrophage polarization. Collectively, these findings support a model in which bacterial EVs mediate the transfer of miR-9394b to coordinate a metabolic-immune response, highlighting a potential avenue for miRNA-guided intervention in experimental colitis.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148016230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0