npj Biofilms and Microbiomes最新文献_第10页

Ulcerative colitis-driven gut dysbiosis exacerbates periodontal bone loss through the gut-oral axis /Th17/Treg imbalance. 溃疡性结肠炎驱动的肠道生态失调通过肠道-口腔轴/Th17/Treg失衡加剧牙周骨质流失。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-23 DOI: 10.1038/s41522-026-01015-6

Yue Huang, Yu Hu, Yifei Zhao, Yuerui Li, Yunkun Liu, Miao Wang, Qian Wang, Huan Hu

{"title":"Ulcerative colitis-driven gut dysbiosis exacerbates periodontal bone loss through the gut-oral axis /Th17/Treg imbalance.","authors":"Yue Huang, Yu Hu, Yifei Zhao, Yuerui Li, Yunkun Liu, Miao Wang, Qian Wang, Huan Hu","doi":"10.1038/s41522-026-01015-6","DOIUrl":"10.1038/s41522-026-01015-6","url":null,"abstract":"Ulcerative colitis (UC) and periodontitis, both microbial dysbiosis-driven chronic inflammatory disorders, coexist and mutually exacerbate, but the causal mechanisms remain unclear. Using ligature-induced periodontitis plus DSS-colitis mice, we found UC doubles alveolar bone loss, heightens systemic inflammation, oxidative stress, and osteoclastogenesis. 16S rRNA and LC-MS metabolomics showed UC enriches oral pathogens, depletes gut Firmicutes, expands Bacteroides, and correlates with suppressed amino-acid/bile-acid biosynthesis. Fecal microbiota transplantation (FMT) from DSS donors into antibiotic-pretreated periodontitis-prone mice replicated aggravated bone loss, systemic inflammation, gut-barrier leakage, and Th17/Treg imbalance, while healthy-donor FMT protected. GC-MS revealed 35-60% reductions in acetate, propionate, and butyrate; keystone taxa Parabacteroides and Muribaculum inversely correlated with SCFAs and host inflammatory genes. Collectively, UC-driven gut dysbiosis is a transmissible causal factor that simultaneously remodels oral and intestinal biofilms, erodes epithelial barriers, and amplifies osteoclastic bone resorption. SCFAs-producing microbes or supplementation may be potential therapeutics for UC-associated periodontitis patients.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13473518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148006368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of gut microbiome with mobility impairment in the Hispanic Community Health Study/Study of Latinos. 西班牙裔社区健康研究/拉丁裔研究中肠道微生物组与活动障碍的关系

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-23 DOI: 10.1038/s41522-026-01019-2

Brandilyn A Peters, Qibin Qi, Xiaonan Xue, Jee-Young Moon, Bing Yu, Stefani N Thomas, Christina Cordero, Martha L Daviglus, Robert D Burk, Robert C Kaplan, Carmen R Isasi

{"title":"Association of gut microbiome with mobility impairment in the Hispanic Community Health Study/Study of Latinos.","authors":"Brandilyn A Peters, Qibin Qi, Xiaonan Xue, Jee-Young Moon, Bing Yu, Stefani N Thomas, Christina Cordero, Martha L Daviglus, Robert D Burk, Robert C Kaplan, Carmen R Isasi","doi":"10.1038/s41522-026-01019-2","DOIUrl":"10.1038/s41522-026-01019-2","url":null,"abstract":"Aging-related declines in mobility are more common in women than men. The gut microbiome may play a role in physical function, but sex-specific roles are unknown. In adults ≥50 years old (n = 1187 women, 585 men), we examined associations of self-reported mobility impairment with gut microbiome assessed by stool shotgun sequencing, and examined heterogeneity by sex. Gut microbiome α-diversity was lower and overall composition (β-diversity) altered in women with mobility impairment compared to without, but this was not the case in men. Fifteen microbiome species were associated with mobility impairment in both women and men, including enrichment of Streptococcus and Lactobacillus and depletion of Eubacterium species. An additional 84 species were associated with mobility impairment in women only, including enrichment of Gammaproteobacteria species, but none were associated with mobility impairment in men only. Correlations of impaired mobility-related microbiome scores, derived from universal and women-specific microbiome species, with serum metabolites (n = 385) suggested that impaired mobility-related species may be involved in synthesis of imidazole propionate and deoxycholic acid metabolites, while species depleted with mobility impairment may be involved in sex hormone metabolism and guanidinoacetate production, the latter in women only. Gut microbiota may play a role in physical function and sex differences therein.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148006280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parabacteroides goldsteinii and its metabolite 7-KLCA attenuate endometriosis via TGR5 to reprogram macrophages by modulating the PPARγ/GPR132 axis. 金斯坦副杆菌及其代谢物7-KLCA通过调节PPARγ/GPR132轴，通过TGR5重编程巨噬细胞，减轻子宫内膜异位症。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-22 DOI: 10.1038/s41522-026-01017-4

Yun Chen, Yuqing Qiu, Feifei Pan, Yanqin Zheng, Jingyao Wang, Kunxiang Gong, Lirong Guo, Yiming Song, Zixin Tao, Kun Shi

{"title":"Parabacteroides goldsteinii and its metabolite 7-KLCA attenuate endometriosis via TGR5 to reprogram macrophages by modulating the PPARγ/GPR132 axis.","authors":"Yun Chen, Yuqing Qiu, Feifei Pan, Yanqin Zheng, Jingyao Wang, Kunxiang Gong, Lirong Guo, Yiming Song, Zixin Tao, Kun Shi","doi":"10.1038/s41522-026-01017-4","DOIUrl":"10.1038/s41522-026-01017-4","url":null,"abstract":"Endometriosis (EMS) remains understudied in effective management strategies. The interplay between macrophage dysfunction and microbiota-derived immune signals emerges as a potential mechanism in EMS pathogenesis, suggesting its relevance for future therapeutic exploration. In this study, we established mouse models to demonstrate that gut microbiota modulated EMS development. Integrated microbial and metabolomic profiling identified Parabacteroides goldsteinii (Pg) as a promising probiotic candidate, whose downstream metabolite 7-ketolithocholic acid (7-KLCA) exhibiting therapeutic efficacy in ameliorating EMS phenotypes upon supplementation. Mechanistically, Pg reshapes its bile acid (BA) metabolism to elevate 7-KLCA. This bioactive metabolite acts via the receptor TGR5 to suppress PPARγ expression and activate GPR132, thereby enhancing efferocytosis and promoting M1 macrophage polarization. These findings uncover a gut-metabolite-immune regulatory axis through which Pg reprograms macrophage function to restrain EMS progression, offering a potential microbial perspective for this chronic condition.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13473097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148006290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetically engineered probiotic E. coli Nissle 1917 enhances protection against Salmonella via increased adhesion and systemic T-cell responses. 基因工程益生菌大肠杆菌Nissle 1917通过增加黏附和全身t细胞反应增强对沙门氏菌的保护。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-21 DOI: 10.1038/s41522-026-01011-w

Ewa Carolak, Joanna Czajkowska, Wiktoria Waszczuk, Agata Dutkiewicz, Anna Ewa Kedzierska, Krzysztof Grzymajlo

{"title":"Genetically engineered probiotic E. coli Nissle 1917 enhances protection against Salmonella via increased adhesion and systemic T-cell responses.","authors":"Ewa Carolak, Joanna Czajkowska, Wiktoria Waszczuk, Agata Dutkiewicz, Anna Ewa Kedzierska, Krzysztof Grzymajlo","doi":"10.1038/s41522-026-01011-w","DOIUrl":"10.1038/s41522-026-01011-w","url":null,"abstract":"The rise in antimicrobial resistance underscores the need for innovative strategies to combat gastrointestinal infections. Probiotics such as Escherichia coli Nissle 1917 (EcN) offer promising options, but the molecular mechanisms underlying their protective effects remain unclear. We introduce a G66R point mutation in FimH, creating a high-binding EcN variant that more effectively prevents Salmonella Typhimurium attachment and induces a distinct host transcriptional profile, shifting toward adaptive rather than innate inflammatory signaling. In vivo, EcNG66R pretreatment significantly reduced intestinal colonization, fecal shedding, and systemic spread, and prevented splenic enlargement compared with EcNWT. Protection was associated with a marked expansion of CD4+ and CD8+ T cells, essential for clearing intracellular pathogens. EcNG66R further enhanced \"readiness\" in the spleen under non-infected conditions, without adverse effects on host physiology. EcNG66R thus functions as a dual-action probiotic-improving competitive exclusion while priming cytotoxic T-cell-mediated protection-and provides a promising platform for developing next-generation microbe-based therapies.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13275912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147988524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaginal microbiome and inflammation cytokines among Chinese women. 中国女性阴道微生物群与炎症细胞因子研究

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-20 DOI: 10.1038/s41522-026-00994-w

Yichan Zhang, Ina Schuppe-Koistinen, Huarui Wang, Xiangyang Tian, Wei-Hua Chen, Guolan Gao, Juan Du

{"title":"Vaginal microbiome and inflammation cytokines among Chinese women.","authors":"Yichan Zhang, Ina Schuppe-Koistinen, Huarui Wang, Xiangyang Tian, Wei-Hua Chen, Guolan Gao, Juan Du","doi":"10.1038/s41522-026-00994-w","DOIUrl":"10.1038/s41522-026-00994-w","url":null,"abstract":"Human papillomavirus (HPV) infection, cervical intraepithelial neoplasia (CIN), pelvic organ prolapse (POP), cervical polyps, abnormal uterine bleeding, and vaginitis are common gynecological disorders. This study characterized the vaginal microbiome and its cytokine-mediated immune interactions in Chinese women with gynecological disorders. Vaginal swabs from 310 patients diagnosed with gynecological disorders and 112 healthy controls were analyzed via 16S rRNA gene sequencing and cytometric bead array (CBA) to evaluate proinflammatory cytokines. Findings on correlation were validated through in vitro co-culture experiments. HPV infection and CIN were associated with dysbiotic microbial profiles and elevated levels of IL-1α, IL-1β, IL-6, IL-8, MCP-1, and MIG. Stratification by microbial composition demonstrated that HPV/CIN patients with non-Lactobacillus-dominant microbiomes harbored the highest IL-1α and IL-1β concentrations. Validated through computational modeling and in vitro analyses, Lactobacillus crispatus and Lactobacillus iners were identified as being strongly inversely correlated with IL-1α and IL-1β expression. Conversely, non-Lactobacillus taxa, including Bifidobacterium breve, Prevotella bivia, Gardnerella vaginalis, Sneathia amnii, Sneathia sanguinegens, Prevotella amnii, Escherichia coli, and Chlamydia trachomatis, exhibited positive correlations with proinflammatory cytokines. These findings highlight a connection between Lactobacillus species and reduced inflammation, while non-Lactobacillus dominance is linked to increased cytokine-driven responses. This study provides valuable insights into microbiome-immune crosstalk in gynecological diseases.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13454521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147982662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing biofilm development, stress response and heterogeneity-spectroscopic characterization of single and multi-species consortia. 探测生物膜发育、胁迫响应和单、多物种群落的异质性光谱表征。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-20 DOI: 10.1038/s41522-026-01010-x

Elena Yunda, Aleksandra Hagberg, Thibault Duteil, Grégory Francius, András Gorzsás, Fabienne Quilès, Madeleine Ramstedt

{"title":"Probing biofilm development, stress response and heterogeneity-spectroscopic characterization of single and multi-species consortia.","authors":"Elena Yunda, Aleksandra Hagberg, Thibault Duteil, Grégory Francius, András Gorzsás, Fabienne Quilès, Madeleine Ramstedt","doi":"10.1038/s41522-026-01010-x","DOIUrl":"10.1038/s41522-026-01010-x","url":null,"abstract":"Environmental bacterial biofilms play many roles in the ecosystem including cycling of nutrients and serving as food for grazing organisms. Their function is linked to their microbial and chemical composition that may be altered by several parameters including environmental stressors. This manuscript presents a well-characterized model system of four bacterial isolates from a small Swedish river: Pseudomonas sp., Sphingomonas sp., Rhizobium sp. and Pararhizobium sp. Microbiological and chemical phenotypes were investigated including cell and biofilm morphology, as well as biochemical composition in absence and presence of the drug trimethoprim. Vibrational spectroscopy, cryo-X-ray photoelectron spectroscopy and confocal optical microscopy were applied to investigate and characterize monocultures and cocultures. The chemical characterization showed variation of the energy storage substance polyhydroxyalkanoates as well as polysaccharides between isolates and drug exposures. Spatial heterogeneities were observed using Raman microspectroscopy where Sphingomonas sp. cells, formed small clusters, inside the four species consortium, an organization that appeared to protect this isolate during exposure to trimethoprim.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"12 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13190702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147982721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug repurposing for glucosyltransferase inhibition for targeted oral biofilm disruption. 靶向口服生物膜破坏中葡萄糖基转移酶抑制的药物再利用。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-16 DOI: 10.1038/s41522-026-01006-7

Fernanda S de Lucena, Matthew G Logan, Steven H Lewis, Hua Zhang, Matthew Barbisan, Marcelo Y Icimoto, Hui Wu, Dustin Higashi, Justin L Merritt, Jonathon L Baker, Carmem S Pfeifer

{"title":"Drug repurposing for glucosyltransferase inhibition for targeted oral biofilm disruption.","authors":"Fernanda S de Lucena, Matthew G Logan, Steven H Lewis, Hua Zhang, Matthew Barbisan, Marcelo Y Icimoto, Hui Wu, Dustin Higashi, Justin L Merritt, Jonathon L Baker, Carmem S Pfeifer","doi":"10.1038/s41522-026-01006-7","DOIUrl":"10.1038/s41522-026-01006-7","url":null,"abstract":"Microbial dysbiosis is the root cause for many diseases. The acidogenic Streptococcus mutans (S.m.) forms a exopolysaccharide-rich biofilm (EPS), mediated by species-specific glucosyltransferases (Gtf), which synthesizes both soluble and insoluble glucans directly on the bacterial surface. GtfC was selected as the primary druggable target since its activity is essential for biofilm initiation and cohesion. Commercial drugs were screened in silico against GtfC, followed by experimental biofilm assays. Two lead compounds, Radotinib and Pranlukast, significantly inhibited GtfC activity and reduced biofilm mass by up to 80. GtfC knock-out models confirmed biofilm disruption specifically via enzyme inhibition. Importantly, Radotinib selectively inhibited S.m. growth while preserving commensal species. This study identified at least two compounds capable of specifically inactivating a primary virulence factor of S.m. without inhibiting its growth, with a much lower selective pressure for drug resistance development, while simultaneously providing a growth advantage to commensal species that promote oral health.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13438108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147960181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthracnose drives assembly of phyllosphere epiphytic bacterial communities to increase disease resistance. 炭疽病驱动层球附生细菌群落的组装，以增加抗病能力。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-15 DOI: 10.1038/s41522-026-00991-z

Rongchun Zheng, Yingde Li, Panpan Shang, Youlei Shen, Zhibiao Nan, Tingyu Duan

{"title":"Anthracnose drives assembly of phyllosphere epiphytic bacterial communities to increase disease resistance.","authors":"Rongchun Zheng, Yingde Li, Panpan Shang, Youlei Shen, Zhibiao Nan, Tingyu Duan","doi":"10.1038/s41522-026-00991-z","DOIUrl":"10.1038/s41522-026-00991-z","url":null,"abstract":"The phyllosphere microbiome plays crucial roles in plant health, but evidence of 'cry for help' strategy in the face of pathogen attack in the phyllosphere remains limited, particularly for the microbiomes of distinct leaf ecological niches. We investigated whether foliar pathogen anthracnose (Colletotrichum lentis) influenced the assembly and functions of microbiomes in epiphytic and endophytic niches of the phyllosphere of common vetch (Vicia sativa) leaves. We also evaluated synthetic microbial communities (SynComs), including representatives of disease-associated strains, for pathogen protection. Anthracnose mediated the deterministic assembly process of epiphytic bacterial and endophytic fungal communities, and increased the complexity of bacterial co-occurrence networks. Iron competition and antifungal genes were also enriched in the epiphytic bacteria, which produce siderophores and degrade fungal cell walls to counteract pathogens. SynComs of beneficial epiphytic bacteria partially protect hosts by regulating bacterial interactions and inducing host immune responses. These findings suggest that disease drives the deterministic assembly of distinct phyllosphere microbiomes, their diversity and their function. Moreover, SynComs from the epiphytic niche can confer host plant disease resistance.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13429687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147948438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Naringin alleviates periodontitis via direct AMPK/Nrf2 activation and NLRP3 inhibition, amplified by gut microbiota/Arg-Gln modulation. 柚皮苷通过直接激活AMPK/Nrf2和抑制NLRP3来缓解牙周炎，并通过肠道微生物群/精氨酸-谷氨酰胺调节放大。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-14 DOI: 10.1038/s41522-026-01007-6

Lan Chuanjian, Zhang Hao, Li Xinyang, Guo Wenjin, Zhao Yang, Wu Qianyu, Feng Hongmingxiu, Liu Zhihui

{"title":"Naringin alleviates periodontitis via direct AMPK/Nrf2 activation and NLRP3 inhibition, amplified by gut microbiota/Arg-Gln modulation.","authors":"Lan Chuanjian, Zhang Hao, Li Xinyang, Guo Wenjin, Zhao Yang, Wu Qianyu, Feng Hongmingxiu, Liu Zhihui","doi":"10.1038/s41522-026-01007-6","DOIUrl":"10.1038/s41522-026-01007-6","url":null,"abstract":"Periodontitis, driven by oral-gut microbiota dysbiosis and NLRP3 inflammasome activation, lacks effective natural therapeutic strategies. This study investigated naringin (Nar), a grapefruit peel flavonoid, using ligature-induced periodontitis in rats and LPS-stimulated RAW264.7 cells. Nar treatment significantly reduced alveolar bone loss, inhibited NLRP3 inflammasome activation (NLRP3, IL-1β), and suppressed inflammatory mediators (COX2, iNOS, IL-6, TNF-α) while improving collagen organisation. Microbiome analysis revealed that Nar suppressed pathogenic bacteria (Veillonella orally, Escherichia-Shigella in the gut) and enriched beneficial Lactobacillus. Metabolomics analysis revealed a significant decrease in the abundance of arginyl-glutamine (arg-gln) in the intestines of rats with periodontitis. Both Nar and arg-gln activated the AMPK/Nrf2 pathway, suppressing NLRP3 activation. FMT from Nar-treated donors had similar anti-inflammatory effects. In conclusion, Nar alleviates periodontitis primarily by directly activating the AMPK/Nrf2 pathway and inhibiting NLRP3 inflammasome activation in periodontal tissues. Additionally, Nar reshapes the gut microbiota to elevate arg-gln levels, which further amplifies AMPK activation and contributes to inflammation control, but is not sufficient alone to drive structural repair. These findings provide a novel theoretical basis for natural compound-mediated microbiota and inflammatory regulation in periodontitis treatment.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13415522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neonatal intensive care unit exposures reprogram microbiome-metabolome trajectories and modulate host calprotectin in preterm infants: a longitudinal multi-omics study. 新生儿重症监护病房暴露重编程微生物代谢组轨迹和调节早产儿宿主钙保护蛋白：一项纵向多组学研究。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-14 DOI: 10.1038/s41522-026-01008-5

Yihuang Huang, Luyang Hong, Shujuan Li, Lan Zhang, Xinhui Guo, Junyan Han, Weiyin Yu, Huiyu Chen, Ningxin Luo, Jinglin Chen, Wenjing Peng, Yufeng Zhou, Shangyu Hong, Weili Yan, Siyuan Jiang, Yun Cao

{"title":"Neonatal intensive care unit exposures reprogram microbiome-metabolome trajectories and modulate host calprotectin in preterm infants: a longitudinal multi-omics study.","authors":"Yihuang Huang, Luyang Hong, Shujuan Li, Lan Zhang, Xinhui Guo, Junyan Han, Weiyin Yu, Huiyu Chen, Ningxin Luo, Jinglin Chen, Wenjing Peng, Yufeng Zhou, Shangyu Hong, Weili Yan, Siyuan Jiang, Yun Cao","doi":"10.1038/s41522-026-01008-5","DOIUrl":"10.1038/s41522-026-01008-5","url":null,"abstract":"Early-life gut microbiota development is critical for orchestrating mucosal barrier function and immune priming, as disruptions in this process can increase susceptibility to life-threatening diseases such as necrotizing enterocolitis (NEC) and sepsis. This longitudinal multi-omics study of 186 preterm infants (<32 weeks of gestation or <1500 g birth weight) explores the impact of early-life exposures in the neonatal intensive care units (NICUs) on gut microbiota, metabolism, and immune responses. We analyzed 1153 stool samples using quantitative microbial profiling, untargeted metabolomics, and fecal S100A8/A9 (calprotectin) levels. Antibiotic exposure suppressed anaerobic colonization and microbial diversity in a cumulative exposure-dependent manner, with breastmilk feeding partially mitigating these effects. The stool metabolome correlated with microbial colonization, showing antibiotic-driven disruptions in polyamine metabolism linked to anaerobe abundance. Host calprotectin levels followed a biphasic pattern, correlating with microbial diversity and polyamine metabolites. Mediation analysis identified anaerobe suppression and polyamine depletion as key drivers of antibiotic-associated reductions in calprotectin. This study reveals that NICU interventions, particularly antibiotics, reprogram the preterm gut ecosystem and immune response, with anaerobes and polyamines being key mediators linking microbial ecology to immune maturation during early life.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13677707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0