npj Biofilms and Microbiomes最新文献

Virome diversity, evolution, transmission networks, and zoonotic potential of wildlife on the Qinghai-Tibet plateau. 青藏高原野生动物病毒多样性、进化、传播网络和人畜共患潜力

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-09 DOI: 10.1038/s41522-026-00993-x

Hao Zhou, Ji Pu, Jinlv Liu, Yaqi Wang, Qiang Hou, Jiajia Ma, Yuqing Liu, Wei Wang, Shan Lu, Jianfeng Ba, Teng Qi, Jing Yang, Xuelian Luo, Jianguo Xu

{"title":"Virome diversity, evolution, transmission networks, and zoonotic potential of wildlife on the Qinghai-Tibet plateau.","authors":"Hao Zhou, Ji Pu, Jinlv Liu, Yaqi Wang, Qiang Hou, Jiajia Ma, Yuqing Liu, Wei Wang, Shan Lu, Jianfeng Ba, Teng Qi, Jing Yang, Xuelian Luo, Jianguo Xu","doi":"10.1038/s41522-026-00993-x","DOIUrl":"https://doi.org/10.1038/s41522-026-00993-x","url":null,"abstract":"Wildlife serves as a natural reservoir for emerging viruses. Although virome studies of bats and rodents have advanced our understanding of viral diversity, the virome characteristics and zoonotic risks of wildlife inhabiting the Qinghai-Tibet Plateau-a global biodiversity hotspot-remain poorly understood. Here, we conducted a virome survey of 22 wild animal species from the Qinghai-Tibet Plateau using meta-transcriptomic sequencing. We characterized the diversity and distribution of wildlife-associated viruses and found that birds tended to harbor higher viral diversity and abundance than mammals. In addition, greylag goose (Anser anser) and Himalayan vulture (Gyps himalayensis) harbored a high proportion of cross-species viruses and occupied central positions in the transmission network. In total, we identified 36 viral genera with pathogenic potential to humans or animals. Among them, several viruses, including rotavirus A (RVA) and parainfluenza virus 5 (PIV-5), pose a high risk of zoonotic transmission. Notably, the plateau vole (Neodon fuscus) carried the highest number of high-risk viruses, and the RVA strains it harbors represent a distinct early evolutionary lineage. These findings highlight the public health relevance of wildlife on the Qinghai-Tibet Plateau and emphasize the need for continued surveillance of high-risk hosts and viruses.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147864239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Instance-based transfer learning enables cross-cohort early detection of colorectal cancer. 基于实例的迁移学习使结直肠癌的跨队列早期检测成为可能。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-09 DOI: 10.1038/s41522-026-01001-y

Yangyang Sun, Shunyao Wu, Zhenming Wu, Wenjie Zhu, Hao Gao, Jieqi Xing, Jin Zhao, Xiaoqian Fan, Xiaoquan Su

{"title":"Instance-based transfer learning enables cross-cohort early detection of colorectal cancer.","authors":"Yangyang Sun, Shunyao Wu, Zhenming Wu, Wenjie Zhu, Hao Gao, Jieqi Xing, Jin Zhao, Xiaoqian Fan, Xiaoquan Su","doi":"10.1038/s41522-026-01001-y","DOIUrl":"https://doi.org/10.1038/s41522-026-01001-y","url":null,"abstract":"Colorectal cancer (CRC) continues to be a major global public health challenge. Extensive research has underscored the critical role of the gut microbiome for diagnostics of CRC. However, early-stage prediction of CRC, particularly at the precancerous adenomas (ADA) stage, remains challenging due to the instability of microbial features across cohorts. In this study, we conducted a systematic analysis of 2053 gut metagenomes from 14 globally-sampled public cohorts and a newly recruited cohort. Despite substantial regional and cohort-level heterogeneity in microbiome composition, we elucidated that the consistent differences between groups in microbial signatures provide the fundamental basis for CRC detection. These patterns enabled robust performance in both inter-cohort and independent validations using an optimized bioinformatics framework. In contrast, such basis was lacking in ADA-associated microbial markers, limiting the generalizability of early detection models. To address this, we developed an instance-based transfer learning approach, Meta-iTL, which effectively leveraged knowledge from existing datasets to detect CRC risk at the ADA stage in the newly recruited cohort. Thus, Meta-iTL overcomes challenges posed by cohort-specific variability and limited data availability and advances the application of non-invasive approaches for the early screening and prevention of CRC.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147864231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary grape pomace mitigates high-NSP-induced inflammation and production loss via microbiome-SCFA-immune mediated pathways. 膳食葡萄渣通过微生物组- scfa免疫介导途径减轻高nsp诱导的炎症和生产损失。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-07 DOI: 10.1038/s41522-026-00996-8

Milan K Sharma, Nikita Agarwal, Sara E Stadulis, Eliot M Dugan, Chloe B Giovannoni, Hannah Glesener, Darya Abdollahzadeh, Haadia Tanveer, Peter R Gracey, Melissa Huang, Patrick A Gibney, Lee E Voth-Gaeddert, Elad Tako

{"title":"Dietary grape pomace mitigates high-NSP-induced inflammation and production loss via microbiome-SCFA-immune mediated pathways.","authors":"Milan K Sharma, Nikita Agarwal, Sara E Stadulis, Eliot M Dugan, Chloe B Giovannoni, Hannah Glesener, Darya Abdollahzadeh, Haadia Tanveer, Peter R Gracey, Melissa Huang, Patrick A Gibney, Lee E Voth-Gaeddert, Elad Tako","doi":"10.1038/s41522-026-00996-8","DOIUrl":"https://doi.org/10.1038/s41522-026-00996-8","url":null,"abstract":"The United States poultry industry is transitioning away from antibiotic growth promoters (AGP) to antibiotic-free programs, which may increase the risk of chronic intestinal inflammation due to exposure to multiple factors. Grape pomace (GP), a polyphenol-rich by-product, might be a promising candidate for mitigating such inflammation. This study investigated the fermented and non-fermented GP as potential substitutes for antibiotic growth promoters in broilers. A total of 126 broilers were divided into six treatment groups: (i) standard diet (STD), (ii) high non-starch polysaccharide diet (NSP), (iii) NSP + zinc bacitracin (AGP), (iv) NSP + 0.5% GP (GP), (v) NSP + 0.5% Lactobacillus casei fermented GP (LAB FGP), and (vi) NSP + 0.5% Saccharomyces cerevisiae fermented GP (YST FGP). NSP-fed birds exhibited reduced growth and increased TNF-α and IL-1β expression, indicating chronic inflammation. GP and FGP suppressed cytokine expression, modulated microbial homeostasis, and increased butyrate production, suggesting functional modulation of the cecal microbiome. Exploratory correlation identified the Lactobacillaceae-butyrate-IL-1β pathway, positively associated with growth, microbiome, and SCFA production, and negatively associated with inflammation. Overall, incorporating 0.5% of GP or FGP into the diet may serve as an effective alternative to AGPs in broiler production, with the added benefits of antioxidants and prebiotics.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila-derived 5-MIAA blunts colitis-associated tumorigenesis by modulating colonic SLC26A3/pSTAT3 signaling. Akkermansia muciniphila衍生的5-MIAA通过调节结肠SLC26A3/pSTAT3信号通路来减弱结肠炎相关的肿瘤发生。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-07 DOI: 10.1038/s41522-026-01000-z

Binhai Shen, Hongbin Liu, Weifeng Huang, Ruo Huang, Qian Zhou, Xinlong Lin, Mengyao Song, Zheng Chen, Yin Zhang, Yinmu Li, Yandong Guo, Lijun Zheng, Yangyang Liu, Fachao Zhi, Wendi Zhang

{"title":"Akkermansia muciniphila-derived 5-MIAA blunts colitis-associated tumorigenesis by modulating colonic SLC26A3/pSTAT3 signaling.","authors":"Binhai Shen, Hongbin Liu, Weifeng Huang, Ruo Huang, Qian Zhou, Xinlong Lin, Mengyao Song, Zheng Chen, Yin Zhang, Yinmu Li, Yandong Guo, Lijun Zheng, Yangyang Liu, Fachao Zhi, Wendi Zhang","doi":"10.1038/s41522-026-01000-z","DOIUrl":"https://doi.org/10.1038/s41522-026-01000-z","url":null,"abstract":"The interaction between gut microbiota and host genetic factors has been implicated in the development of colitis-associated colorectal cancer (CAC). Although Akkermansia muciniphila (Akk) has been studied in CAC, its role and molecular mechanism remain undetermined. In this study, we found that antibiotics treatment effectively inhibited tumorigenesis in azoxymethane/dextran sulfate sodium (AOM/DSS)-induced CAC mice, and was associated with an increase in Akk's relative abundance in feces. Akk supplementation, as well as its conditioned medium, remarkably restrained tumor formation in the CAC model, whereas the original medium did not yield the same results. Further untargeted metabolomics and germ-free mice experiments revealed that Akk generated a tryptophan metabolite 5-methoxyindole-3-acetic acid (5-MIAA), which demonstrated protective effects against CAC proliferation. Mechanistically, 5-MIAA boosted colonic SLC26A3 expression to suppress the phosphorylation of STAT3 (pSTAT3), thus inhibiting CAC proliferation, which was further validated by conditional knockout mice. Collectively, we revealed that a tryptophan metabolite 5-MIAA, derived from Akk, inhibited CAC proliferation through colonic SLC26A3/pSTAT3 signaling pathway, which indicated that Akk and its functional metabolites may serve as novel therapeutic approaches for the prevention and treatment of CAC.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics atlas of the bovine coronavirus-infected calf jejunum: reduction of Phocaeicola coprophilus and deoxycholic acid linked to Th17/Treg imbalance. 牛冠状病毒感染小牛空肠的多组学图谱：与Th17/Treg失衡相关的Phocaeicola coprophilus和脱氧胆酸的减少

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-07 DOI: 10.1038/s41522-026-00997-7

Zheng Niu, Jingyi Xu, Xinfeng Hou, Guanglei Liu, Junhu Yao, Yangchun Cao, Qian Du, Dewen Tong, Shengru Wu

{"title":"Multi-omics atlas of the bovine coronavirus-infected calf jejunum: reduction of Phocaeicola coprophilus and deoxycholic acid linked to Th17/Treg imbalance.","authors":"Zheng Niu, Jingyi Xu, Xinfeng Hou, Guanglei Liu, Junhu Yao, Yangchun Cao, Qian Du, Dewen Tong, Shengru Wu","doi":"10.1038/s41522-026-00997-7","DOIUrl":"https://doi.org/10.1038/s41522-026-00997-7","url":null,"abstract":"Optimal calf rearing is fundamental for ensuring efficient milk production. Bovine coronavirus (BCoV) poses a significant threat to calf health and leads to substantial economic losses in the dairy industry. However, the mechanisms by which the intestinal mucosal microbiome regulates the host immune response during infection remain unclear. In this study, we constructed a high-resolution map of the jejunal mucosal microenvironment in BCoV-infected calves. Our findings revealed that BCoV infection led to severe microbial dysbiosis, characterized by a marked reduction in Phocaeicola coprophilus (formerly known as Bacteroides coprophilus) and decreased secondary bile acid, especially deoxycholic acid (DCA). Concurrently, enrichment of harmful microbiota correlated with increased arachidonic acid metabolites. At the host level, BCoV infection altered the composition of jejunal mucosal cells and affected metabolic and immune-related pathways. The differentiation of CD4⁺ T cells played a central role in the jejunum's response to BCoV infection. By integrating these metabolic alterations with dynamic host cellular responses, we suggested a putative that DCA deficiency might contribute to the pathological polarization of CD4+ T cells toward a Th17 phenotype while suppressing Treg differentiation. These findings suggest that restoring the Phocaeicola coprophilus-affected bile acid transformation might represent a promising therapeutic strategy for BCoV infection.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota-GABA axis dysregulation underlies polystyrene microplastic (PS-MP) neurotoxicity in rainbow trout: a role for oxidative stress and blood-brain barrier disruption. 肠道微生物- gaba轴失调是虹鳟聚苯乙烯微塑料（PS-MP）神经毒性的基础：氧化应激和血脑屏障破坏的作用。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-07 DOI: 10.1038/s41522-026-00995-9

Fang Ma, Pan Zheng, Wenli Wang, Jiaxuan Dong, Xiangjun Zhou, Zhiyun Lin, Xiajiao Nian

{"title":"Gut microbiota-GABA axis dysregulation underlies polystyrene microplastic (PS-MP) neurotoxicity in rainbow trout: a role for oxidative stress and blood-brain barrier disruption.","authors":"Fang Ma, Pan Zheng, Wenli Wang, Jiaxuan Dong, Xiangjun Zhou, Zhiyun Lin, Xiajiao Nian","doi":"10.1038/s41522-026-00995-9","DOIUrl":"https://doi.org/10.1038/s41522-026-00995-9","url":null,"abstract":"Microplastics (MPs) threaten aquatic ecosystems and pose potential risks to organismal health through bioaccumulation in aquatic species. This study reveals that 14-day exposure to 5 μm polystyrene microplastics (PS-MPs) (500 μg/L) induces neurocognitive impairment in rainbow trout (Oncorhynchus mykiss), a globally consumed aquaculture species. MPs accumulated in brain and gut tissues, causing blood-brain barrier structural alterations, intestinal mucosal damage, and oxidative stress. Multi-omics analysis revealed associations between gut microbiota dysbiosis (reduced Ralstonia, increased Acinetobacter) to suppressed neuroactive pathways, particularly GABA synthesis and transport. Downregulation of monocarboxylate transporters (mct1/2) and GABA-related enzymes (GAD1/2) disrupted gut-to-brain GABA homeostasis, neurobehavioral deficits. These findings establish the gut microbiota-GABA axis as a critical mediator of MPs neurotoxicity, highlighting risks to seafood safety and necessitating urgent regulation of microplastic contamination in aquatic food chains.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung microbiota-mediated biotransformation of mogroside preserves pulmonary barrier integrity and attenuates PM_2.5-induced inflammation via NF-κB-Th17 modulations. 肺微生物介导的甜菊苷生物转化通过NF-κB-Th17调节，保持肺屏障完整性，减轻pm2.5诱导的炎症。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-05 DOI: 10.1038/s41522-026-00992-y

Kai Wang, Yuan Li, Cuiguang Li, Mohammed Kahiel, Kentaro Nagaoka, Dan Shen, Chunmei Li

{"title":"Lung microbiota-mediated biotransformation of mogroside preserves pulmonary barrier integrity and attenuates PM2.5-induced inflammation via NF-κB-Th17 modulations.","authors":"Kai Wang, Yuan Li, Cuiguang Li, Mohammed Kahiel, Kentaro Nagaoka, Dan Shen, Chunmei Li","doi":"10.1038/s41522-026-00992-y","DOIUrl":"https://doi.org/10.1038/s41522-026-00992-y","url":null,"abstract":"PM2.5-induced lung injury challenges poultry health with limited treatments. Mogroside's unique therapeutic impact on pulmonary inflammation may involve modulating the lung microbiome, which influences immune function and respiratory health. We first demonstrated that mogroside (MG) supplementation improved growth performance and mitigated PM2.5-induced alveolar damage, inflammatory cytokine release, and Th17 differentiation (p < 0.05). MG increased the abundance of beneficial bacteria, particularly Lactobacillus (p < 0.01). Notably, MG IIE accumulated in lung tissues and bronchoalveolar lavage fluid (BALF). To further clarify the role of microbe-metabolite interactions, BALF from MG-treated broilers was transplanted. Only complete BALF containing both MG and microbiota significantly alleviated fibrosis (p < 0.05), reshaped lung microbial composition, and modulated metabolites such as taurine and lactic acid. Microbiome analysis identified Sphingomonas as a key taxon enriched in MG-BALF, strongly correlated with protective metabolites. In vitro assays confirmed that Sphingomonas degraded MG IIE into mogrol via β-glucosidase activity. Finally, a Calu-3-Jurkat T lymphocytes co-culture model revealed that MG IIE, particularly in combination with Sphingomonas metabolites, preserved barrier integrity, suppressed NF-κB phosphorylation, reduced ROS, and inhibited Th17-associated cytokine expression. Collectively, MG IIE and its Sphingomonas-mediated metabolites form a lung microbiota-metabolite-host axis that protects against PM2.5-induced pulmonary injury.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-specific microbial and tryptophan signatures of depression implicate archaeal methanogens and indole-3-acetic acid only in women. 抑郁症的性别特异性微生物和色氨酸特征仅在女性中涉及古细菌产甲烷菌和吲哚-3-乙酸。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-05-04 DOI: 10.1038/s41522-026-00983-z

Anna Motger-Albertí, Laura Gallardo-Nuell, Marisel Rosell-Díaz, Mihaela Stefoglo, Javier Pons, Josep Garre-Olmo, Vicente Pérez-Brocal, Andrés Moya, Josep Puig, Rafael Ramos, Jordi Mayneris-Perxachs, José Manuel Fernández-Real

{"title":"Sex-specific microbial and tryptophan signatures of depression implicate archaeal methanogens and indole-3-acetic acid only in women.","authors":"Anna Motger-Albertí, Laura Gallardo-Nuell, Marisel Rosell-Díaz, Mihaela Stefoglo, Javier Pons, Josep Garre-Olmo, Vicente Pérez-Brocal, Andrés Moya, Josep Puig, Rafael Ramos, Jordi Mayneris-Perxachs, José Manuel Fernández-Real","doi":"10.1038/s41522-026-00983-z","DOIUrl":"https://doi.org/10.1038/s41522-026-00983-z","url":null,"abstract":"Sex differences in mental health are often overlooked, yet gut microbiota-host metabolite interactions may contribute to sexual dimorphism in depression. In a population-based cohort, we investigated sex-specific links among plasma tryptophan metabolites, depressive symptoms (PHQ-9), and the gut microbiome, controlling for smoking, diet, alcohol, and physical activity. Women (N = 419) exhibited higher plasma indole-3-acetic acid (IAA) and picolinic acid (PA) concentrations, but lower trigonelline (TRIG) than men (N = 383). Machine learning models with SHAP explanations revealed that IAA and TRIG were positively associated, whereas PA was negatively associated with depression severity in women, whereas only kynurenic acid (KA) was inversely associated in men. In women, depression severity strongly correlated with methanogenic archaea, including Methanobrevibacter smithii, and microbial methane-production pathways. Methanobrevibacter and specifically M. smithii were negatively linked to genes for tryptophan, PA, and KA biosynthesis, but positively to genes for IAA and nicotinate/nicotinamide metabolism. Most microbial species associated with depression severity in women were predicted to produce IAA. These findings reveal pronounced sex-specific microbiome-metabolite interactions, highlighting potentially distinct microbial mechanisms shaping depression in men and women.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intermittent fasting alleviates hyperalgesia in ovariectomized mice via gut microbiota remodeling. 间歇性禁食通过肠道菌群重塑减轻去卵巢小鼠的痛觉过敏。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-04-29 DOI: 10.1038/s41522-026-00990-0

Rui-Feng Ao, Yu-Sheng Huang, Ying-Tao Hu, Heng-Rui Yong, Shi-Cheng Wang, Hong-Zhou Li, Jia-Wen Gao, Chen Tu, Jing-Shen Zhuang, Zhao-Ming Zhong

{"title":"Intermittent fasting alleviates hyperalgesia in ovariectomized mice via gut microbiota remodeling.","authors":"Rui-Feng Ao, Yu-Sheng Huang, Ying-Tao Hu, Heng-Rui Yong, Shi-Cheng Wang, Hong-Zhou Li, Jia-Wen Gao, Chen Tu, Jing-Shen Zhuang, Zhao-Ming Zhong","doi":"10.1038/s41522-026-00990-0","DOIUrl":"https://doi.org/10.1038/s41522-026-00990-0","url":null,"abstract":"Pain is a common symptom of menopause, yet effective therapeutic options are limited. Intermittent fasting (IF) has emerged as a promising dietary intervention; however, its effects on menopausal pain are still unclear. In this study, we established a hyperalgesia model in mice through ovariectomy (OVX) and subjected them to an alternate-day fasting regimen. IF significantly elevated the pain thresholds for mechanical, hot and cold stimuli in OVX mice and reduced the expression of pain-related molecules, including transient receptor potential vanilloid 1 (TRPV1) and calcitonin gene-related peptide (CGRP) in dorsal root ganglion (DRG). Moreover, IF remodeled the gut microbiota and metabolite profile, marked by a substantial increase in the abundance of Akkermansia muciniphila and its key metabolite, indole-3-propionic acid (IPA). Depletion of the gut microbiota via antibiotic treatment abolished the analgesic effects of IF on OVX-induced hyperalgesia. Conversely, fecal microbiota transplantation from IF-treated donors restored microbial composition and alleviated hyperalgesia in OVX recipients. Administration of A. muciniphila increased IPA levels and alleviated hyperalgesia in OVX mice. Importantly, exogenous IPA supplementation not only alleviated hyperalgesia but also reduced the excitability of DRG neurons. Together, these findings demonstrate that IF mitigates estrogen deficiency-related hyperalgesia through remodeling gut microbiota and metabolite profile, and identify IPA as a potential therapeutic target, offering new perspectives for the clinical management of menopausal pain.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":9.2,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147777537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gradient metapopulation microfluidic ecologies shape genetic and biofilm drivers of T4r phage resistance in E. coli. 梯度元群体微流控生态塑造了大肠杆菌T4r噬菌体耐药性的遗传和生物膜驱动因素。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-04-25 DOI: 10.1038/s41522-026-00959-z

Krisztina Nagy, Sarshad Koderi Valappil, Trung V Phan, Shengkai Li, Laszlo Der, Ryan Morris, Julia Bos, Sophia Winslow, Peter Galajda, Gabor Rakhely, Robert H Austin

引用次数: 0