npj Biofilms and Microbiomes最新文献_第2页

Microfluidics unveils role of gravity and shear stress on Pseudomonas fluorescens motility and biofilm growth. 微流体揭示了重力和剪切应力对荧光假单胞菌运动和生物膜生长的作用。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-01 DOI: 10.1038/s41522-025-00744-4

Daniele Marra, Moreno Rizzo, Sergio Caserta

引用次数: 0

Lactobacillus regulate muscle fiber type conversion in Chinese native pigs via tryptophan metabolism. 乳酸菌通过色氨酸代谢调节猪肌纤维类型转化。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-23 DOI: 10.1038/s41522-025-00745-3

Bo Song, Md Abul Kalam Azad, Qian Zhu, Yating Cheng, Sujuan Ding, Kang Yao, Xiangfeng Kong

引用次数: 0

A promiscuous Bcd amino acid dehydrogenase promotes biofilm development in Bacillus subtilis. 混杂的Bcd氨基酸脱氢酶促进枯草芽孢杆菌生物膜的发育。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-21 DOI: 10.1038/s41522-025-00750-6

David Ranava, Stephen M Lander, Szu-Yu Kuan, Jonathan D Winkelman, Arthur Prindle, Mee-Ngan F Yap

{"title":"A promiscuous Bcd amino acid dehydrogenase promotes biofilm development in Bacillus subtilis.","authors":"David Ranava, Stephen M Lander, Szu-Yu Kuan, Jonathan D Winkelman, Arthur Prindle, Mee-Ngan F Yap","doi":"10.1038/s41522-025-00750-6","DOIUrl":"10.1038/s41522-025-00750-6","url":null,"abstract":"Glutamate dehydrogenase (GDH) resides at the crossroads of nitrogen and carbon metabolism, catalyzing the reversible conversion of L-glutamate to α-ketoglutarate and ammonium. GDH paralogs are ubiquitous across most species, presumably enabling functional specialization and genetic compensation in response to diverse conditions. Staphylococcus aureus harbors a single housekeeping GDH (GudB), whereas Bacillus subtilis encodes both a major and a minor GDH, GudB and RocG, respectively. In an unsuccessful attempt to identify an alternative GDH in S. aureus, we serendipitously discovered previously unrecognized GDH activity in two metabolic enzymes of B. subtilis. The hexameric Val/Leu/Ile dehydrogenase Bcd (formerly YqiT) catabolizes branched-chain amino acids and to a lesser extent glutamate using NAD+ as a cofactor. Removal of gudB and rocG unmasks the dual NAD(P)+-dependent GDH activity of RocA, which otherwise functions as a 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase. Bcd homologs are prevalent in free-living and obligate bacteria but are absent in most, if not all, staphylococci. Despite low sequence homology, Bcd structurally resembles the GudB/RocG family and can functionally compensate for the loss of GudB in S. aureus. Bcd is essential for the full maturation of biofilms. B. subtilis lacking GDHs exhibits severe impairments in rugose architecture and colony expansion of biofilms. This study underscores the importance of metabolic redundancy and highlights the critical role of substrate promiscuity in GDHs during biofilm development.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"112"},"PeriodicalIF":7.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactobacillus amylovorus extracellular vesicles mitigate mammary gland ferroptosis via the gut-mammary gland axis. 淀粉样乳杆菌胞外囊泡通过肠-乳腺轴减轻乳腺铁下垂。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-21 DOI: 10.1038/s41522-025-00752-4

Qianzi Zhang, Dongpang Chen, Hanting Ding, Qihui Li, Siyu Yuan, Haobin Li, Wutai Guan, Shihai Zhang

{"title":"Lactobacillus amylovorus extracellular vesicles mitigate mammary gland ferroptosis via the gut-mammary gland axis.","authors":"Qianzi Zhang, Dongpang Chen, Hanting Ding, Qihui Li, Siyu Yuan, Haobin Li, Wutai Guan, Shihai Zhang","doi":"10.1038/s41522-025-00752-4","DOIUrl":"10.1038/s41522-025-00752-4","url":null,"abstract":"Lactation is essential for supporting neonatal growth and development, and its regulation is influenced by the gut microbiota. However, the role of gut microbes in lactation under conditions of oxidative stress remains unclear. In this study, we identify a novel function for gut microbiota in regulating maternal lactation through the modulation of ferroptosis in the mammary gland under oxidative stress. We identify Lactobacillus amylovorus (L. amylovorus), enriched in mothers with low oxidative stress, as negatively correlating with both oxidative stress and ferroptosis. In a mouse model, L. amylovorus alleviates mammary ferroptosis and promotes lactation. In addition to producing of short-chain fatty acids, L. amylovorus secretes bacterial extracellular vesicles (BEVs) enriched in oleic acid, a monounsaturated fatty acid that can be transferred to the mammary gland. Mechanistically, the accumulation of oleic acid in mammary epithelial cells enhances their resistance to ferroptosis, thereby supporting milk production. These findings highlight the potential of L. amylovorus and its BEVs as therapeutic tools to counteract oxidative stress-induced lactation decline.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"113"},"PeriodicalIF":7.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic evidence for flies as carriers of zoonotic pathogens on dairy farms. 苍蝇作为奶牛农场人畜共患病病原体携带者的基因组证据。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-19 DOI: 10.1038/s41522-025-00685-y

Andrew J Sommer, Joseph H Skarlupka, Serafino Teseo, Saria Otani, Garret Suen, Kerri L Coon, Panagiotis Sapountzis

{"title":"Genomic evidence for flies as carriers of zoonotic pathogens on dairy farms.","authors":"Andrew J Sommer, Joseph H Skarlupka, Serafino Teseo, Saria Otani, Garret Suen, Kerri L Coon, Panagiotis Sapountzis","doi":"10.1038/s41522-025-00685-y","DOIUrl":"10.1038/s41522-025-00685-y","url":null,"abstract":"Dairy farms are major reservoirs of zoonotic bacterial pathogens, which harbor antimicrobial resistance genes (ARGs), and raise critical questions about their dissemination on and off the farm environment. Here, we investigated the role of coprophagous muscid flies (Diptera: Muscidae) as carriers of zoonotic pathogens and antimicrobial resistance. We collected cow manure and flies on a dairy farm and used shotgun metagenomics to identify the presence of clinically relevant bacteria, virulence factors, and ARGs in both environments. Our results reveal that, although the fly microbiome is largely composed of manure-associated taxa, they also harbor specific insect-associated bacteria, which may be involved in nutrient provisioning to the host. Furthermore, we identifed shared ARGs, virulence factors, and zoonotic pathogens enriched within the fly gastrointestinal tract (GIT). Our study illustrates the potential flow of pathogenic microorganisms from manure to coprophagous flies, suggesting that flies may pose an important zoonotic threat on dairy farms.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"111"},"PeriodicalIF":7.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tanzanian gut microbiota profiles linked to high but rapidly waning yellow fever antibody titers. 坦桑尼亚的肠道微生物群与黄热病抗体滴度高但迅速下降有关。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-19 DOI: 10.1038/s41522-025-00687-w

Jeremia J Pyuza, Marloes M A R van Dorst, David Barnett, Koen Stam, Mikhael Manurung, Linda Wammes, Marion König, Yvonne Kruize, Nikuntufya Andongolile, Anastazia Ngowi, Elichilia R Shao, Vesla I Kullaya, Alex Mremi, Pancras C W Hogendoorn, Sia E Msuya, Simon P Jochems, John Penders, Maria Yazdanbakhsh, Wouter A A de Steenhuijsen Piters

{"title":"Tanzanian gut microbiota profiles linked to high but rapidly waning yellow fever antibody titers.","authors":"Jeremia J Pyuza, Marloes M A R van Dorst, David Barnett, Koen Stam, Mikhael Manurung, Linda Wammes, Marion König, Yvonne Kruize, Nikuntufya Andongolile, Anastazia Ngowi, Elichilia R Shao, Vesla I Kullaya, Alex Mremi, Pancras C W Hogendoorn, Sia E Msuya, Simon P Jochems, John Penders, Maria Yazdanbakhsh, Wouter A A de Steenhuijsen Piters","doi":"10.1038/s41522-025-00687-w","DOIUrl":"10.1038/s41522-025-00687-w","url":null,"abstract":"Vaccine responses vary across populations and are influenced by numerous intrinsic and extrinsic factors, including the gut microbiota. However, studies linking microbiota composition to vaccine immunogenicity in low- and middle-income countries are sparse. In this study, we examined the gut microbiota of 143 healthy rural and urban living Tanzanians who participated in a yellow fever vaccine (YF-17D) trial. We found significant differences in gut microbiota profiles between rural and urban participants. Rural-associated microbiota showed higher diversity and enrichment of taxa like Prevotella and Succinivibrio, which were linked to dietary intake patterns. Yellow fever neutralizing antibody titers were higher in rural compared to urban participants. Interestingly, a subset of urban individuals with a rural-like microbiota had higher antibody titers and faster antibody waning than those with a more industrialized microbiota. These findings suggest that gut microbiota composition might be linked to vaccine immunogenicity, potentially outweighing the influence of living location.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"110"},"PeriodicalIF":7.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

M.marinum lacking epsH shows increased biofilm formation in vitro and boosted antibiotic tolerance in zebrafish. 缺乏epsH的marinum在斑马鱼中显示出体外生物膜形成增加和抗生素耐受性增强。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-14 DOI: 10.1038/s41522-025-00743-5

Saara Lehmusvaara, Alina Sillanpää, Milan Wouters, Rosa Korhonen, Nelli Vahvelainen, Hanna Luukinen, Paulina Deptula, Kirsi Savijoki, Milka Hammarén, Mataleena Parikka

{"title":"M.marinum lacking epsH shows increased biofilm formation in vitro and boosted antibiotic tolerance in zebrafish.","authors":"Saara Lehmusvaara, Alina Sillanpää, Milan Wouters, Rosa Korhonen, Nelli Vahvelainen, Hanna Luukinen, Paulina Deptula, Kirsi Savijoki, Milka Hammarén, Mataleena Parikka","doi":"10.1038/s41522-025-00743-5","DOIUrl":"10.1038/s41522-025-00743-5","url":null,"abstract":"Recent discoveries have indicated that biofilm communities may play a role in natural drug tolerance of Mycobacterium tuberculosis. A transposon-based mutation library of a closely related species, Mycobacterium marinum, was used to identify clones in which the relative amount of extracellular DNA (eDNA), an important component of the extracellular matrix of biofilms, is altered. The disruption of a putative glycosyl transferase gene QDR78 11175, epsH, caused a substantial increase of the eDNA content of biofilms, and increased the growth rate and the biomass/cell in biofilm-forming conditions compared to wild-type. The increased abundance of biomass was mainly due to the elevated levels of eDNA and proteins in the extracellular matrix. The growth of the ΔepsH strain in the zebrafish was normal, but the mutant developed greater antibiotic tolerance in the adult zebrafish model. These results suggest that the extracellular matrix of biofilms increases antibiotic tolerance of mycobacteria during infection.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"109"},"PeriodicalIF":7.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular DNA filaments associated with surface polysaccharide II give Clostridioides difficile biofilm matrix a network-like structure. 胞外DNA细丝与表面多糖II相结合，使艰难梭菌生物膜基质具有网状结构。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-13 DOI: 10.1038/s41522-025-00751-5

Tania Kamwouo, Sylvie Bouttier, Séverine Domenichini, Johanna Saunier, Héloïse Coullon, Alexis Simons, Claire Janoir

{"title":"Extracellular DNA filaments associated with surface polysaccharide II give Clostridioides difficile biofilm matrix a network-like structure.","authors":"Tania Kamwouo, Sylvie Bouttier, Séverine Domenichini, Johanna Saunier, Héloïse Coullon, Alexis Simons, Claire Janoir","doi":"10.1038/s41522-025-00751-5","DOIUrl":"10.1038/s41522-025-00751-5","url":null,"abstract":"Clostridioides difficile is an anaerobic, spore-forming, Gram-positive bacterium, and a leading cause of healthcare-associated intestinal infections. Recurrences occur frequently, most of them being relapses. Apart from spores, C. difficile biofilm is hypothesized as a reservoir for relapses. Thus, increased knowledge on in vitro biofilm formation and characteristics is required. We finely characterized the matrix components in 4 C. difficile strains. Confocal microscopy revealed for the first time the presence of eDNA filaments connecting bacteria, with a spider's web-like organization. Biofilm disruption with DNase I suggests that eDNA, even in low abundance, plays a key role in the biofilm scaffold, maintaining biofilm cohesion by connecting bacteria. Observation of strong overlapping staining, particularly in the highest biofilm-producing strain tested between eDNA and polysaccharide II or lipoprotein CD1687, suggests that interactions between these components may enhance biofilm cohesion. Whereas autolysis does not appear to be a major way of matrix component release under our conditions, eDNA was sometimes associated with lipidic round shapes that can evoke vesicle structures. Together, these results suggest that the bacterial aggregation and structuring of the C. difficile biofilm involve several components of the matrix, including eDNA, interacting with each other to build the scaffold of biofilm.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"108"},"PeriodicalIF":7.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice. 邻苯二甲酸二（2-乙基己基）酯通过破坏小鼠微生物-肠道-下丘脑-垂体-卵巢轴降低妊娠率。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-13 DOI: 10.1038/s41522-025-00742-6

Linjie Xu, Jun Yan, Tingting Yin, Yingzi Pan, Min Chen, Xinyan Wang, Lan Wu, Hongjuan Ding

{"title":"Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice.","authors":"Linjie Xu, Jun Yan, Tingting Yin, Yingzi Pan, Min Chen, Xinyan Wang, Lan Wu, Hongjuan Ding","doi":"10.1038/s41522-025-00742-6","DOIUrl":"10.1038/s41522-025-00742-6","url":null,"abstract":"Di (2-ethylhexyl) Phthalate (DEHP), a widely used plasticizer and endocrine disruptor, poses risks to human health, particularly reproductive function. Using a mouse model, we investigated how DEHP exposure impacts the hypothalamic-pituitary-ovarian (HPO) axis through gut microbiome disruption. DEHP decreased pregnancy rates by impairing ovarian function, activating hypothalamic astrocytes, and increasing neuregulin 1 (NRG1) expression. NRG1 binding to astrocyte ErbB2 receptors elevated prostaglandin E2 (PGE2) and gonadotropin-releasing hormone (GnRH), disrupting HPO axis homeostasis. Additionally, DEHP altered gut microbiota, destabilized microbial networks, and impacted β-glucuronidase-related taxa, leading to hormone fluctuations and reduced fertility. This study highlights gut microbiome perturbations as a novel mechanism linking DEHP exposure to reproductive dysfunction. Our study provides novel insights concerning perturbations of the gut microbiome and HPO axis and their functions as a potential new mechanism by which DEHP exposes interferes with the reproductive function-related human health.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"107"},"PeriodicalIF":7.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota alterations are linked to COVID-19 severity in North African and European populations. 在北非和欧洲人群中，肠道微生物群的改变与COVID-19的严重程度有关。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-13 DOI: 10.1038/s41522-025-00733-7

Marius Bredon, Pierre Hausfater, Loubna Khalki, Youssef Tijani, Amine Cheikh, Loic Brot, Laura Creusot, Nathalie Rolhion, Francois Trottein, Gérard Lambeau, Sophie Georgin-Lavialle, Alexandre Bleibtreu, Jean-Luc Baudel, Antoine Lefèvre, Patrick Emond, Florence Tubach, Noémie Simon-Tillaux, Tabassome Simon, Guy Gorochov, Younes Zaid, Harry Sokol

{"title":"Gut microbiota alterations are linked to COVID-19 severity in North African and European populations.","authors":"Marius Bredon, Pierre Hausfater, Loubna Khalki, Youssef Tijani, Amine Cheikh, Loic Brot, Laura Creusot, Nathalie Rolhion, Francois Trottein, Gérard Lambeau, Sophie Georgin-Lavialle, Alexandre Bleibtreu, Jean-Luc Baudel, Antoine Lefèvre, Patrick Emond, Florence Tubach, Noémie Simon-Tillaux, Tabassome Simon, Guy Gorochov, Younes Zaid, Harry Sokol","doi":"10.1038/s41522-025-00733-7","DOIUrl":"10.1038/s41522-025-00733-7","url":null,"abstract":"Although COVID-19 primarily affects the respiratory system, many patients experience gastrointestinal symptoms, suggesting a role for the gut microbiota in disease pathogenesis. To explore this, we performed shotgun metagenomic sequencing on stool samples from 200 COVID-19 patients and 102 healthy controls in Morocco and France. Despite geographic differences in microbiota composition, patients with COVID-19 in both continents exhibited significant gut microbiota alterations, which were more pronounced in severe cases, with similar features compared with controls. Functional pathways, including L-Tryptophan biosynthesis, were disrupted, particularly in patients with severe disease. Machine learning models accurately predicted disease severity based on gut microbial profiles in the Moroccan cohort, though not in the French cohort. These results highlight consistent microbiota changes associated with COVID-19 and support a potential link between gut dysbiosis and disease severity.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"106"},"PeriodicalIF":7.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0