npj Biofilms and Microbiomes最新文献_第2页

Characterizing interactions of Staphylococcus aureus and Escherichia coli in dual-species implant-associated biofilms. 表征金黄色葡萄球菌和大肠杆菌在双种植入物相关生物膜中的相互作用。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-26 DOI: 10.1038/s41522-025-00754-2

Amita Sekar, Fawaz Ben Malick, Shweta Uma Deepak, Nicoletta Inverardi, Dillon Murugesan, Orhun K Muratoglu, Ebru Oral

{"title":"Characterizing interactions of Staphylococcus aureus and Escherichia coli in dual-species implant-associated biofilms.","authors":"Amita Sekar, Fawaz Ben Malick, Shweta Uma Deepak, Nicoletta Inverardi, Dillon Murugesan, Orhun K Muratoglu, Ebru Oral","doi":"10.1038/s41522-025-00754-2","DOIUrl":"10.1038/s41522-025-00754-2","url":null,"abstract":"While Staphylococcus aureus is the predominant pathogen in periprosthetic joint infections (PJI), polymicrobial infections involving Gram-negative organisms, such as Escherichia coli, complicate clinical outcomes. Little is known regarding implant-associated polymicrobial interactions; consequently, current PJI treatments are not optimized for their treatment. This study explored the dynamics of S. aureus-E. coli dual-species biofilms, focusing on biofilm properties, antibiotic susceptibility, and molecular interactions. Co-culture experiments revealed that E. coli significantly suppressed S. aureus biofilm viability, observed for methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). Microscopic analyses demonstrated enhanced E. coli attachment facilitated by S. aureus matrix proteins; however, over time, E. coli dominated the biofilm composition. In the presence of E. coli, MSSA biofilm exhibited improved gentamicin susceptibility while MRSA showed limited change, underscoring strain-specific interactions. Notably, E. coli biofilms exhibited enhanced resistance to gentamicin in dual-species settings. Gene expression profiling revealed molecular adaptation in S. aureus and E. coli, triggered by the differential regulation of stress, adhesion, virulence, and biofilm-associated genes within a dual-species implant-associated biofilm. The suppression of S. aureus by E. coli presents potential therapeutic avenues, and in vivo studies and mechanistic investigations are crucial for optimizing treatment strategies targeting polymicrobial PJIs.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"189"},"PeriodicalIF":9.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12475442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prokaryotic evolution shapes specialized communities in long term engineered pit mud ecosystem. 原核生物的进化在长期工程坑泥生态系统中形成了专门的群落。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-24 DOI: 10.1038/s41522-025-00805-8

Yan Zeng, Xiaozhong Zhong, Lijuan Chai, Xiaojuan Zhang, Zhenming Lu, Guangqian Liu, Tingyao Tu, Lingfei Lu, Rui Zhang, Hui Yu, Suyi Zhang, Songtao Wang, Caihong Shen, Jinsong Shi, Zhenghong Xu

{"title":"Prokaryotic evolution shapes specialized communities in long term engineered pit mud ecosystem.","authors":"Yan Zeng, Xiaozhong Zhong, Lijuan Chai, Xiaojuan Zhang, Zhenming Lu, Guangqian Liu, Tingyao Tu, Lingfei Lu, Rui Zhang, Hui Yu, Suyi Zhang, Songtao Wang, Caihong Shen, Jinsong Shi, Zhenghong Xu","doi":"10.1038/s41522-025-00805-8","DOIUrl":"10.1038/s41522-025-00805-8","url":null,"abstract":"Elucidating the temporal dynamics of complex microbial consortia is crucial for engineering robust microbiome. We investigated prokaryotic evolution in pit mud, a centuries-old engineered environment used in Chinese liquor fermentation. Metagenomic analysis of 120 pit mud samples across different ages revealed a transition from generalist-dominated to specialist-enriched communities. This shift was characterized by decreased hydrolytic potential and increased organic acid metabolism, with key taxonomic changes including declines in Proteiniphilum and Petrimonas, and increases in Methanobacterium and Caproicibacter. The mature specialist community accelerates the short-chain organic acids turnover through syntrophic fatty acid oxidation, methanogenesis, and carbon chain elongation, maintaining ecosystem stability. While nutrient availability primarily shapes early stages community interactions, environmental stress becomes a dominant factor in mature systems. These insights into long-term prokaryotic adaptation provide a foundation for the rational design of resilient, functionally optimized microbial communities for biotechnological applications.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"186"},"PeriodicalIF":9.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and antibacterial evaluation of trisindolines against methicillin-resistant Staphylococcus aureus targeting cell membrane. 三吲哚啉对靶向细胞膜的耐甲氧西林金黄色葡萄球菌的合成及抑菌评价。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-24 DOI: 10.1038/s41522-025-00739-1

Arti Rathore, Irshad Ahmad Zargar, Jyoti Kumari, Biplab Sarkar, Rakshit Manhas, Shifa Firdous, Ramajayan Pandian, Debaraj Mukherjee, Avisek Mahapa

{"title":"Synthesis and antibacterial evaluation of trisindolines against methicillin-resistant Staphylococcus aureus targeting cell membrane.","authors":"Arti Rathore, Irshad Ahmad Zargar, Jyoti Kumari, Biplab Sarkar, Rakshit Manhas, Shifa Firdous, Ramajayan Pandian, Debaraj Mukherjee, Avisek Mahapa","doi":"10.1038/s41522-025-00739-1","DOIUrl":"10.1038/s41522-025-00739-1","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant global health threat that requires novel antimicrobials to combat this WHO-designated priority pathogen. In this study, we designed, synthesized and evaluated a series of unexplored trisindoline derivatives against MRSA, including multidrug-resistant (MDR) clinical isolates. The Structure Activity Relationship (SAR) analysis of the trisindolines indicated the importance of strategic substitutions in the trisindoline core for their anti-staphylococcal efficacy. Biocompatibility studies revealed a high safety profile for the active compounds across various mammalian cell lines. Furthermore, the derivatives displayed rapid bactericidal action, anti-biofilm efficacy, intracellular MRSA killing and combinatorial effect with vancomycin. Mechanistic studies revealed that these compounds disrupt MRSA cell integrity by influencing several membrane-related pathways. Finally, in vivo assessments of a lead trisindoline in an MRSA-induced systemic infection model demonstrated a significant reduction of bacterial load. Therefore, these trisindoline molecules may offer a promising therapeutic model for combating MRSA infections.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"184"},"PeriodicalIF":9.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lacticaseibacillus rhamnosus GR-1 prevents autism-like behaviors by reshaping the maternal and offspring microbiome. 鼠李糖乳杆菌GR-1通过重塑母体和后代的微生物群来预防自闭症样行为。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-24 DOI: 10.1038/s41522-025-00808-5

Ruili Yang, Yi Xu, Jinchun Xu, Chengqing Huang, Feng Zhu, Tian Wang, Rui Kong, Jie Xiao, Biao He, Xiaozhen Gu, Hui-Li Wang

{"title":"Lacticaseibacillus rhamnosus GR-1 prevents autism-like behaviors by reshaping the maternal and offspring microbiome.","authors":"Ruili Yang, Yi Xu, Jinchun Xu, Chengqing Huang, Feng Zhu, Tian Wang, Rui Kong, Jie Xiao, Biao He, Xiaozhen Gu, Hui-Li Wang","doi":"10.1038/s41522-025-00808-5","DOIUrl":"10.1038/s41522-025-00808-5","url":null,"abstract":"As a prevalent neurodevelopmental disease, whether ASD (autism spectrum disorder) can be ameliorated by the early use of a single microbe remains unknown. Here we used a lactobacillus strain, Lacticaseibacillus rhamnosus GR-1 (LGR-1), for prenatal intervention in autism-like mice with either environmental or idiopathic origins by exclusively administering to the pregnant dams at a dose of 109/mouse/day, followed by offspring behavioral assessment with 3-chamber trial and marble burying test. The results revealed that LGR-1 prevented the occurrence of autism-like symptoms, as evidenced by the improved behaviors and restored E/I (excitatory-inhibitory) balance in the prefrontal cortex of male pups. In parallel, the offspring microbiome was reshaped by LGR-1 treatment, probably mediated by the vertical transmission of maternal microbiome, with its roles further unraveled by fecal microbiota transplant and cross-fostering experiments. In addition to gut commensals, the LGR-1-shaping vaginal microbiota also contributed to the establishment of \"beneficial\" microbiome. Regarding key taxa in offspring, Akkermansia muciniphila was influenced by LGR-1 and exerted impact on behaviors via pathways related to IL-17-producing lymphocytes. Our findings demonstrate that prenatal microbial administration protects offspring against autism-like behavioral phenotypes through microbiome transmission, highlighting a potential microbe-based therapeutic avenue to mitigate ASD risk.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"187"},"PeriodicalIF":9.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rationally designed microbial consortium modulates neurodegeneration in a Drosophila melanogaster model of Parkinson's disease. 合理设计的微生物联合体调节帕金森病黑胃果蝇模型的神经退行性变。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-24 DOI: 10.1038/s41522-025-00797-5

Aline Ovalle, Estefanía López, Jimena Sierralta, Nuria Paricio, Daniel Garrido

{"title":"A rationally designed microbial consortium modulates neurodegeneration in a Drosophila melanogaster model of Parkinson's disease.","authors":"Aline Ovalle, Estefanía López, Jimena Sierralta, Nuria Paricio, Daniel Garrido","doi":"10.1038/s41522-025-00797-5","DOIUrl":"10.1038/s41522-025-00797-5","url":null,"abstract":"The gut-brain axis enables communication between the central nervous system and the gut, with certain microbial metabolites influencing neurodegeneration. Using genome-scale metabolic modeling, we designed and tested a synthetic microbial consortium with predicted capacity to produce GABA and other neurometabolites in a Drosophila melanogaster model of neurodegeneration of Parkinson's Disease (PD). The consortium (Levilactobacillus brevis, Lacticaseibacillus paracasei, Bacteroides thetaiotaomicron) produced GABA in bioreactors. Female PD flies receiving the consortium exhibited protection from locomotor impairment at 10 and 25 days upon consortium administration. Head metabolomics revealed partial restoration of PD-associated alterations in energy balance, amino acid and neurotransmitter metabolism, and disease-related biomarkers. Early administration increased microbiome diversity and Lactobacillus abundance. These findings suggest that rationally designed microbial consortia targeting neurometabolite production can modulate brain physiology and confer neuroprotection, supporting their potential for microbiome-based interventions in neurodegenerative disease.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"185"},"PeriodicalIF":9.2,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ABO blood group antigens influence host-microbe interactions and risk of early spontaneous preterm birth. ABO血型抗原影响宿主-微生物相互作用和早期自发性早产的风险。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-10 DOI: 10.1038/s41522-025-00783-x

Katherine E Mountain, David A MacIntyre, Yun S Lee, Virginia Tajadura-Ortega, Anne Dell, Stuart M Haslam, Gang Wu, Paola Grassi, Ten Feizi, Yan Liu, Wengang Chai, Julian R Marchesi, Lauren A Roberts, Denise Chan, Belen Gimeno-Molina, Richard G Brown, Holly V Lewis, Alice K C Hyde, James Pasint-Magyar, Anna Green, Anna L David, Jane E Norman, Sarah J Stock, Samit Kundu, Sherrianne Ng, Ben Glampson, Erik Mayer, T G Teoh, Vasso Terzidou, Phillip R Bennett, Lynne Sykes

{"title":"ABO blood group antigens influence host-microbe interactions and risk of early spontaneous preterm birth.","authors":"Katherine E Mountain, David A MacIntyre, Yun S Lee, Virginia Tajadura-Ortega, Anne Dell, Stuart M Haslam, Gang Wu, Paola Grassi, Ten Feizi, Yan Liu, Wengang Chai, Julian R Marchesi, Lauren A Roberts, Denise Chan, Belen Gimeno-Molina, Richard G Brown, Holly V Lewis, Alice K C Hyde, James Pasint-Magyar, Anna Green, Anna L David, Jane E Norman, Sarah J Stock, Samit Kundu, Sherrianne Ng, Ben Glampson, Erik Mayer, T G Teoh, Vasso Terzidou, Phillip R Bennett, Lynne Sykes","doi":"10.1038/s41522-025-00783-x","DOIUrl":"10.1038/s41522-025-00783-x","url":null,"abstract":"The mechanisms by which vaginal microbiota shape spontaneous preterm birth (sPTB) risk remain poorly defined. Using electronic clinical records data from 74,913 maternities in conjunction with metaxanomic (n = 596) and immune profiling (n = 314) data, we show that the B blood group phenotype associates with increased risk of sPTB and adverse vaginal microbiota composition. The O blood group associates with sPTB in women who have a combination of a previous history of sPTB, an adverse vaginal microbial composition and pro-inflammatory cervicovaginal milieu. In contrast, women of blood group A have a higher prevalence of vaginal Lactobacillus crispatus, a lower risk of sPTB, with sPTB cases showing no association with vaginal microbiota composition or inflammation. We found that cervicovaginal fluid contains ABH(O) glycans and shows variable binding to key vaginal bacteria. This indicates that cervicovaginal ABH(O) glycans influence microbiota-host interactions implicated in sPTB risk, suggesting a novel target for sPTB prediction and prevention.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"170"},"PeriodicalIF":9.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metatranscriptomics-based metabolic modeling of patient-specific urinary microbiome during infection. 基于超转录组学的感染期间患者特异性尿微生物组代谢模型。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-09 DOI: 10.1038/s41522-025-00823-6

Jonathan Josephs-Spaulding, Hannah Clara Rettig, Johannes Zimmermann, Mariam Chkonia, Alexander Mischnik, Sören Franzenburg, Simon Graspeuntner, Jan Rupp, Christoph Kaleta

{"title":"Metatranscriptomics-based metabolic modeling of patient-specific urinary microbiome during infection.","authors":"Jonathan Josephs-Spaulding, Hannah Clara Rettig, Johannes Zimmermann, Mariam Chkonia, Alexander Mischnik, Sören Franzenburg, Simon Graspeuntner, Jan Rupp, Christoph Kaleta","doi":"10.1038/s41522-025-00823-6","DOIUrl":"10.1038/s41522-025-00823-6","url":null,"abstract":"Urinary tract infections (UTIs) are among the most common bacterial infections and are increasingly complicated by multidrug resistance (MDR). While Escherichia coli is frequently implicated, the contribution of broader microbial communities remains less understood. Here, we integrate metatranscriptomic sequencing with genome-scale metabolic modeling to characterize active metabolic functions of patient-specific urinary microbiomes during acute UTI. We analyzed urine samples from 19 female patients with confirmed uropathogenic E. coli (UPEC) infections, reconstructing personalized community models constrained by gene expression and simulated in a virtual urine environment. This systems biology approach revealed marked inter-patient variability in microbial composition, transcriptional activity, and metabolic behavior. We identified distinct virulence strategies, metabolic cross-feeding, and a modulatory role for Lactobacillus species. Comparisons between transcript-constrained and unconstrained models showed that integrating gene expression narrows flux variability and enhances biological relevance. These findings highlight the metabolic heterogeneity of UTI-associated microbiota and point to microbiome-informed diagnostic and therapeutic strategies for managing MDR infections.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"183"},"PeriodicalIF":9.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling novel microbial players in the breast tissue of TNBC patients: a meta-analytic perspective. 揭示TNBC患者乳腺组织中新的微生物玩家：一个元分析的角度。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-09 DOI: 10.1038/s41522-025-00816-5

Hannah H Rashwan, Nour H Marzouk, Rana A Youness, Mohamed El-Hadidi, Raghda Ramadan, Mohamed Mysara

{"title":"Unravelling novel microbial players in the breast tissue of TNBC patients: a meta-analytic perspective.","authors":"Hannah H Rashwan, Nour H Marzouk, Rana A Youness, Mohamed El-Hadidi, Raghda Ramadan, Mohamed Mysara","doi":"10.1038/s41522-025-00816-5","DOIUrl":"10.1038/s41522-025-00816-5","url":null,"abstract":"Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer (BC), accounting for nearly 40% of BC-related deaths. Emerging evidence suggests that the breast tissue microbiome harbors distinct microbial communities; however, the microbiome specific to TNBC remains largely unexplored. This study presents the first comprehensive meta-analysis of the TNBC tissue microbiome, consolidating 16S rRNA amplicon sequencing data from 200 BC samples across four independent cohorts. Our analysis highlights the enrichment of Azospirillum genus as well as butyrate-producing species, namely Gemmiger formicilis and Anaerobutyricum soehngenii, potentially influencing TNBC aggressiveness and clinical outcomes. Additionally, our functional analyses reveal the involvement of the TNBC microbiome in several pathways associated with chronic inflammation, increased cellular proliferation, invasion, and metastasis. This study uncovers novel microbial players in TNBC that could explain its aggressiveness and poor prognosis, and warrants further investigation into microbiome-driven interventions.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"182"},"PeriodicalIF":9.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Algae-dominated metaproteomes uncover cellular adaptations to life on the Greenland Ice Sheet. 藻类主导的元蛋白质组揭示了细胞对格陵兰冰盖上生命的适应。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-09 DOI: 10.1038/s41522-025-00770-2

Helen K Feord, Anke Trautwein-Schult, Christoph Keuschnig, Anne Ostrzinski, Elisa K Peter, Carsten Jaeger, Jan Lisec, Rey Mourot, Ravi Sven Peters, Ozan Çiftçi, Martyn Tranter, Alexandre M Anesio, Dörte Becher, Liane G Benning

{"title":"Algae-dominated metaproteomes uncover cellular adaptations to life on the Greenland Ice Sheet.","authors":"Helen K Feord, Anke Trautwein-Schult, Christoph Keuschnig, Anne Ostrzinski, Elisa K Peter, Carsten Jaeger, Jan Lisec, Rey Mourot, Ravi Sven Peters, Ozan Çiftçi, Martyn Tranter, Alexandre M Anesio, Dörte Becher, Liane G Benning","doi":"10.1038/s41522-025-00770-2","DOIUrl":"10.1038/s41522-025-00770-2","url":null,"abstract":"Eukaryotic algae-dominated microbiomes thrive on the Greenland Ice Sheet (GrIS) in harsh environmental conditions, including low temperatures, high light, and low nutrient availability. Chlorophyte algae bloom on snow, while streptophyte algae dominate bare ice surfaces. Empirical data about the cellular mechanisms responsible for their survival in these extreme conditions are scarce. This knowledge gap was addressed by quantifying proteins for both algal taxa from samples on the southern margin of the GrIS. We show that the streptophyte glacier ice algae have a relative enrichment in proteins involved in environmental signaling and nutrient transport, indicative of cellular readiness to dynamically respond to extreme GriS environmental cues, linked, for example, to photoprotection and the rapid update of scarce nutrients. In contrast, the chlorophyte snow algae have a high abundance of proteins linked to lipid and nitrogen metabolisms, providing evidence for the biological processes sustaining the cellular carbon and nitrogen stores necessary for survival in an oligotrophic environment. We also identify proteins in both taxa linked to the synthesis and breakdown of key cellular pigments. Our study gives novel insights into the cellular biology of these algae and their adaptation to extreme environments.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"181"},"PeriodicalIF":9.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12420790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome meets immunotherapy: unlocking the hidden predictors of immune checkpoint inhibitors. 微生物组与免疫疗法：解开免疫检查点抑制剂的隐藏预测因子。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-09-02 DOI: 10.1038/s41522-025-00819-2

Lihaoyun Huang, Yu Li, Chunyan Zhang, Aimin Jiang, Lingxuan Zhu, Weiming Mou, Kailai Li, Jian Zhang, Cui Cui, Xinfang Cui, Anqi Lin, Peng Luo, Ting Wei

{"title":"Microbiome meets immunotherapy: unlocking the hidden predictors of immune checkpoint inhibitors.","authors":"Lihaoyun Huang, Yu Li, Chunyan Zhang, Aimin Jiang, Lingxuan Zhu, Weiming Mou, Kailai Li, Jian Zhang, Cui Cui, Xinfang Cui, Anqi Lin, Peng Luo, Ting Wei","doi":"10.1038/s41522-025-00819-2","DOIUrl":"10.1038/s41522-025-00819-2","url":null,"abstract":"Immune checkpoint inhibitors (ICIs) have revolutionized cancer immunotherapy, with the microbiome significantly influencing treatment outcomes. Analysis of 4663 studies (2014.01-2024.10) identified 71 eligible randomized controlled trials (RCTs) and cohort studies (41 viral, 30 bacterial). Analyses included subgroup assessments by cancer type, microbial taxa, and ICI regimens. Among 4663 identified studies, 71 met inclusion criteria (41 viral, 30 bacterial). Viral status, particularly hepatitis B virus (HBV) and human papillomavirus (HPV), significantly associated with ORR and DCR. Bacterial enrichment correlated with improved survival in hepatobiliary (OS: HR = 4.33, 95%CI: 2.20-8.50) and lung cancers (PFS: HR = 1.70, 95%CI: 1.04-2.78). Multi-microbiome models demonstrated superior outcome prediction, with microbial diversity correlating with improved PFS (HR = 0.64, 95%CI: 0.42-0.98). Viral status showed cancer-specific associations with SAEs. The microbiome serves as a valuable predictor of ICI outcomes. Future studies should emphasize large-scale RCTs, standardized assessment methods, and host-microbiome interactions.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"180"},"PeriodicalIF":9.2,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0