npj Biofilms and Microbiomes最新文献_第3页

Extracellular DNA filaments associated with surface polysaccharide II give Clostridioides difficile biofilm matrix a network-like structure. 胞外DNA细丝与表面多糖II相结合，使艰难梭菌生物膜基质具有网状结构。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-13 DOI: 10.1038/s41522-025-00751-5

Tania Kamwouo, Sylvie Bouttier, Séverine Domenichini, Johanna Saunier, Héloïse Coullon, Alexis Simons, Claire Janoir

{"title":"Extracellular DNA filaments associated with surface polysaccharide II give Clostridioides difficile biofilm matrix a network-like structure.","authors":"Tania Kamwouo, Sylvie Bouttier, Séverine Domenichini, Johanna Saunier, Héloïse Coullon, Alexis Simons, Claire Janoir","doi":"10.1038/s41522-025-00751-5","DOIUrl":"10.1038/s41522-025-00751-5","url":null,"abstract":"Clostridioides difficile is an anaerobic, spore-forming, Gram-positive bacterium, and a leading cause of healthcare-associated intestinal infections. Recurrences occur frequently, most of them being relapses. Apart from spores, C. difficile biofilm is hypothesized as a reservoir for relapses. Thus, increased knowledge on in vitro biofilm formation and characteristics is required. We finely characterized the matrix components in 4 C. difficile strains. Confocal microscopy revealed for the first time the presence of eDNA filaments connecting bacteria, with a spider's web-like organization. Biofilm disruption with DNase I suggests that eDNA, even in low abundance, plays a key role in the biofilm scaffold, maintaining biofilm cohesion by connecting bacteria. Observation of strong overlapping staining, particularly in the highest biofilm-producing strain tested between eDNA and polysaccharide II or lipoprotein CD1687, suggests that interactions between these components may enhance biofilm cohesion. Whereas autolysis does not appear to be a major way of matrix component release under our conditions, eDNA was sometimes associated with lipidic round shapes that can evoke vesicle structures. Together, these results suggest that the bacterial aggregation and structuring of the C. difficile biofilm involve several components of the matrix, including eDNA, interacting with each other to build the scaffold of biofilm.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"108"},"PeriodicalIF":7.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice. 邻苯二甲酸二（2-乙基己基）酯通过破坏小鼠微生物-肠道-下丘脑-垂体-卵巢轴降低妊娠率。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-13 DOI: 10.1038/s41522-025-00742-6

Linjie Xu, Jun Yan, Tingting Yin, Yingzi Pan, Min Chen, Xinyan Wang, Lan Wu, Hongjuan Ding

{"title":"Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice.","authors":"Linjie Xu, Jun Yan, Tingting Yin, Yingzi Pan, Min Chen, Xinyan Wang, Lan Wu, Hongjuan Ding","doi":"10.1038/s41522-025-00742-6","DOIUrl":"10.1038/s41522-025-00742-6","url":null,"abstract":"Di (2-ethylhexyl) Phthalate (DEHP), a widely used plasticizer and endocrine disruptor, poses risks to human health, particularly reproductive function. Using a mouse model, we investigated how DEHP exposure impacts the hypothalamic-pituitary-ovarian (HPO) axis through gut microbiome disruption. DEHP decreased pregnancy rates by impairing ovarian function, activating hypothalamic astrocytes, and increasing neuregulin 1 (NRG1) expression. NRG1 binding to astrocyte ErbB2 receptors elevated prostaglandin E2 (PGE2) and gonadotropin-releasing hormone (GnRH), disrupting HPO axis homeostasis. Additionally, DEHP altered gut microbiota, destabilized microbial networks, and impacted β-glucuronidase-related taxa, leading to hormone fluctuations and reduced fertility. This study highlights gut microbiome perturbations as a novel mechanism linking DEHP exposure to reproductive dysfunction. Our study provides novel insights concerning perturbations of the gut microbiome and HPO axis and their functions as a potential new mechanism by which DEHP exposes interferes with the reproductive function-related human health.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"107"},"PeriodicalIF":7.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiota alterations are linked to COVID-19 severity in North African and European populations. 在北非和欧洲人群中，肠道微生物群的改变与COVID-19的严重程度有关。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-13 DOI: 10.1038/s41522-025-00733-7

Marius Bredon, Pierre Hausfater, Loubna Khalki, Youssef Tijani, Amine Cheikh, Loic Brot, Laura Creusot, Nathalie Rolhion, Francois Trottein, Gérard Lambeau, Sophie Georgin-Lavialle, Alexandre Bleibtreu, Jean-Luc Baudel, Antoine Lefèvre, Patrick Emond, Florence Tubach, Noémie Simon-Tillaux, Tabassome Simon, Guy Gorochov, Younes Zaid, Harry Sokol

{"title":"Gut microbiota alterations are linked to COVID-19 severity in North African and European populations.","authors":"Marius Bredon, Pierre Hausfater, Loubna Khalki, Youssef Tijani, Amine Cheikh, Loic Brot, Laura Creusot, Nathalie Rolhion, Francois Trottein, Gérard Lambeau, Sophie Georgin-Lavialle, Alexandre Bleibtreu, Jean-Luc Baudel, Antoine Lefèvre, Patrick Emond, Florence Tubach, Noémie Simon-Tillaux, Tabassome Simon, Guy Gorochov, Younes Zaid, Harry Sokol","doi":"10.1038/s41522-025-00733-7","DOIUrl":"10.1038/s41522-025-00733-7","url":null,"abstract":"Although COVID-19 primarily affects the respiratory system, many patients experience gastrointestinal symptoms, suggesting a role for the gut microbiota in disease pathogenesis. To explore this, we performed shotgun metagenomic sequencing on stool samples from 200 COVID-19 patients and 102 healthy controls in Morocco and France. Despite geographic differences in microbiota composition, patients with COVID-19 in both continents exhibited significant gut microbiota alterations, which were more pronounced in severe cases, with similar features compared with controls. Functional pathways, including L-Tryptophan biosynthesis, were disrupted, particularly in patients with severe disease. Machine learning models accurately predicted disease severity based on gut microbial profiles in the Moroccan cohort, though not in the French cohort. These results highlight consistent microbiota changes associated with COVID-19 and support a potential link between gut dysbiosis and disease severity.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"106"},"PeriodicalIF":7.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune system dynamics in response to Pseudomonas aeruginosa biofilms. 免疫系统动力学对铜绿假单胞菌生物膜的反应。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-12 DOI: 10.1038/s41522-025-00738-2

Abhijeet Sahu, Rohit Ruhal

引用次数: 0

Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18. 对感染HPV 16和18的女性阴道微生物组的宏基因组研究

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-12 DOI: 10.1038/s41522-025-00747-1

Da-Ryung Jung, Yeseul Choi, Minsoo Jeong, Vineet Singh, Se Young Jeon, Incheol Seo, Nora Jee-Young Park, Yoon Hee Lee, Ji Young Park, Hyung Soo Han, Jae-Ho Shin, Gun Oh Chong

{"title":"Metagenomic insight into the vaginal microbiome in women infected with HPV 16 and 18.","authors":"Da-Ryung Jung, Yeseul Choi, Minsoo Jeong, Vineet Singh, Se Young Jeon, Incheol Seo, Nora Jee-Young Park, Yoon Hee Lee, Ji Young Park, Hyung Soo Han, Jae-Ho Shin, Gun Oh Chong","doi":"10.1038/s41522-025-00747-1","DOIUrl":"10.1038/s41522-025-00747-1","url":null,"abstract":"Human papillomavirus (HPV) 16 and 18 (HPV 16/18) account for over 70% of cervical cancer (CC) cases, yet their interaction with the vaginal microbiome (VM) remains unclear. This study explored the association between high-risk HPV types (HR-HPVs), VM composition and bacterial function using shotgun metagenomic sequencing. In early-stage cervical lesions, the HPV 16/18 group showed reduced Lactobacillus-dominant community state types compared to other HR-HPVs, while invasive CC exhibited increased pathogenic bacteria, including Streptococcus agalactiae, Fannyhessea vaginae, and Sneathia vaginalis. The VM associated with HPV 16/18 was enriched in immune response and inflammation pathways, whereas other HR-HPVs were linked to cellular metabolism and hormonal signaling. Notably, HPV 16/18 exhibited stronger bacterial-fungal correlations, indicating shifts in the microbial community. Furthermore, 137 metagenome-assembled genomes provided insights into unique microbial genomic signatures. Our study links VM differences with HPV 16/18 oncogenic potential across cervical lesion stages, urging further research for better diagnostics and treatments.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"105"},"PeriodicalIF":7.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diffusion-based mechanism explains spatial organization in cross-feeding biofilms. 以扩散为基础的机制解释了交叉取食生物膜的空间组织。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-11 DOI: 10.1038/s41522-025-00719-5

Julio Pérez, Cristian Picioreanu

{"title":"Diffusion-based mechanism explains spatial organization in cross-feeding biofilms.","authors":"Julio Pérez, Cristian Picioreanu","doi":"10.1038/s41522-025-00719-5","DOIUrl":"10.1038/s41522-025-00719-5","url":null,"abstract":"Complex symbiotic interactions were claimed for explaining spatial organization of microbial species in cross-feeding biofilms. Here however, a distinct mechanism is proposed, called diffusion-based enhanced microbial organization (DEMO). An accepted mathematical model based on one-dimensional balances with diffusion-reaction of substrates and convection of multiple microbial types in a cross-feeding biofilm was used to describe emerging microbial distributions. The model allowed isolation of the effects of diffusion from other factors (kinetics, stoichiometry, specific symbiotic interactions), pointing to a possible mechanism for stratification in anaerobic biofilms. The secondary degrader consuming waste metabolite from a primary degrader was retained in anaerobic biofilms in an apparent growth yield disproportion. However, diffusion of an intermediate substrate can be responsible for this disproportion, even in longer food chains. This microbial distribution was not observed in independent feeding. In aerobic biofilms, this mechanism remains inactive, explaining the preference for full oxidation of organic matter in aerobic degradation.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"102"},"PeriodicalIF":7.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive genome catalog analysis of the resistome, virulome and mobilome in the wild rodent gut microbiota. 野生啮齿动物肠道微生物群中抵抗组、病毒组和活动组的综合基因组目录分析。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-11 DOI: 10.1038/s41522-025-00746-2

Kai-Meng Shang, He Ma, Hany M Elsheikha, Yong-Jie Wei, Ji-Xin Zhao, Ya Qin, Jian-Ming Li, Zi-Yu Zhao, Xiao-Xuan Zhang

{"title":"Comprehensive genome catalog analysis of the resistome, virulome and mobilome in the wild rodent gut microbiota.","authors":"Kai-Meng Shang, He Ma, Hany M Elsheikha, Yong-Jie Wei, Ji-Xin Zhao, Ya Qin, Jian-Ming Li, Zi-Yu Zhao, Xiao-Xuan Zhang","doi":"10.1038/s41522-025-00746-2","DOIUrl":"10.1038/s41522-025-00746-2","url":null,"abstract":"Wild rodent's gut microbiota serves as a crucial reservoir of antibiotic resistance genes (ARGs), where antimicrobial-resistant bacteria interact with mobile genetic elements (MGEs) to facilitate horizontal gene transfer. This study analyzed 12,255 gut-derived bacterial genomes from wild rodents to characterize the distribution of ARGs and virulence factor genes (VFGs), and to identify their bacterial hosts. A total of 8119 ARGs and 7626 VFGs were identified. The most prevalent ARGs conferred resistance to elfamycin, followed by those associated with multi-class antibiotic resistance. Enterobacteriaceae, particularly Escherichia coli, harbored the highest numbers of ARGs and VFGs. A strong correlation between the presence of MGEs, ARGs, and VFGs was observed, highlighting the potential for co-selection and mobilization of resistance and virulence traits. These findings underscore the importance of expanded surveillance to monitor and mitigate the risk of transmission of resistant and potentially pathogenic bacteria from wild rodents to human and animal populations.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"101"},"PeriodicalIF":7.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virome characterization of field-collected rodents in suburban city. 郊区野外采集鼠类病毒组特征分析。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-11 DOI: 10.1038/s41522-025-00740-8

Zhen-Yu Hu, Fang Tang, Mei-Qi Zhang, Jing-Tao Zhang, Yun-Fa Zhang, Guang-Qian Si, Bo-Kang Fu, Gang Wang, Shuang Li, Lei Zhang, Cong Peng, Xiao-Fang Ma, Yan-Tao Liu, Hong Su, Peng-Tao Bao, Xiao-Ai Zhang, Wei Liu

{"title":"Virome characterization of field-collected rodents in suburban city.","authors":"Zhen-Yu Hu, Fang Tang, Mei-Qi Zhang, Jing-Tao Zhang, Yun-Fa Zhang, Guang-Qian Si, Bo-Kang Fu, Gang Wang, Shuang Li, Lei Zhang, Cong Peng, Xiao-Fang Ma, Yan-Tao Liu, Hong Su, Peng-Tao Bao, Xiao-Ai Zhang, Wei Liu","doi":"10.1038/s41522-025-00740-8","DOIUrl":"10.1038/s41522-025-00740-8","url":null,"abstract":"Rodents serve as natural reservoirs and vectors for a multitude of zoonotic viruses. Analyzing the viral diversity in rodents is crucial for predicting the emergence of infectious diseases. Through high-throughput sequencing, we characterized the RNA virome of nine rodent species from the families Cricetidae and Muridae, collected from suburban Beijing (n = 432). The composition of the virome exhibited significant variation at the virus family level across the nine rodent species and three types of natural habitats. A total of 142 viral species were identified, including 133 associated with vertebrates and 9 associated with invertebrates, spanning 26 families. Notably, 75 of these viruses were novel while 67 were known viruses. Twenty-five viruses were classified as high-risk, including eight zoonotic viruses and 17 spillover-risk viruses. Additionally, nine known viruses were identified for the first time in China. Thirty-three viruses demonstrated potential for cross-species transmission. Understanding the virome characterization of field-collected rodent might provide valuable insights into the potential risk of zoonotic spillover to humans.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"103"},"PeriodicalIF":7.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12159166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient. 揭示沿着城市化梯度驱动土壤和地衣微生物组组装的生态过程。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-10 DOI: 10.1038/s41522-025-00736-4

Panji Cahya Mawarda, Rens van der Kaaij, Francisco Dini-Andreote, Deniz Duijker, Michael Stech, Adrianus Gcl Speksnijder

{"title":"Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient.","authors":"Panji Cahya Mawarda, Rens van der Kaaij, Francisco Dini-Andreote, Deniz Duijker, Michael Stech, Adrianus Gcl Speksnijder","doi":"10.1038/s41522-025-00736-4","DOIUrl":"10.1038/s41522-025-00736-4","url":null,"abstract":"Global biodiversity loss is accelerating due to the transformation of natural landscapes into agricultural and urban areas. Yet, research on the urbanization impact on environmental and host-associated microbiomes, particularly on the ecological processes that mediate their assembly and function, remains scarce. This study investigated the effects of an urbanization gradient on the diversity and assembly processes of the soil microbiome and the microbiomes of three epiphytic lichen species (Candelaria concolor, Physcia adscendens, and Xanthoria parietina). Our findings revealed that the urbanization gradient shaped the soil microbiome, while the lichen microbiomes exhibited strong host specificity and showed no significant changes in diversity along the urbanization gradient. Heterogeneous selection and dispersal limitation primarily governed the soil community assembly and higher community turnover in medium- and highly urbanized zones compared to low-urbanized zones, indicating an increased influence of environmental pressures, altered resources, and habitat fragmentation in more urbanized areas. The lichen microbiome assembly in each species was primarily governed by undominated processes regardless of urbanization level, indicating that both selection and stochasticity contributed to, but neither dominantly influenced, their assembly. The lichen microbiomes further revealed species-specific co-occurrence networks, with microbial compositional signatures and potential functions being essential for lichen fitness and urban ecosystem health. Taken together, our study contributes to understanding how microbial communities are assembled in urban environments, bridging the gap between conceptual theories and empirical findings in the urban ecology of soil and lichen-associated microbiomes.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"99"},"PeriodicalIF":7.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polybacterial intracellular macromolecules shape single-cell inflammatory profiles in upper airway epithelia. 多细菌细胞内大分子形成上呼吸道上皮单细胞炎症谱。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-06-10 DOI: 10.1038/s41522-025-00735-5

Quinn T Easter, Zabdiel Alvarado-Martinez, Meik Kunz, Bruno F Matuck, Brittany T Rupp, Theresa Weaver, Zhi Ren, Aleksandra Tata, Juan Caballero-Perez, Nick Oscarson, Akira Hasuike, Ameer N Ghodke, Adam J Kimple, Purushothama R Tata, Scott H Randell, Hyun Koo, Kang I Ko, Kevin M Byrd

{"title":"Polybacterial intracellular macromolecules shape single-cell inflammatory profiles in upper airway epithelia.","authors":"Quinn T Easter, Zabdiel Alvarado-Martinez, Meik Kunz, Bruno F Matuck, Brittany T Rupp, Theresa Weaver, Zhi Ren, Aleksandra Tata, Juan Caballero-Perez, Nick Oscarson, Akira Hasuike, Ameer N Ghodke, Adam J Kimple, Purushothama R Tata, Scott H Randell, Hyun Koo, Kang I Ko, Kevin M Byrd","doi":"10.1038/s41522-025-00735-5","DOIUrl":"10.1038/s41522-025-00735-5","url":null,"abstract":"Mucosal epithelial cells of the upper airways are continuously exposed to microbes throughout life. Specialized niches such as the anterior nares and the tooth are especially susceptible to dysbiosis and chronic inflammatory diseases. Here, we reanalyzed our v1-Human Periodontal Atlas, identifying polybacterial signatures (20% Gram-positive; 80% Gram-negative) and distinct responses of bacterial-associated epithelia. Fluorescence microscopy detected numerous persistent polybacterial intracellular macromolecules (PIMs) within human oral keratinocytes (HOKs), including bacterial rRNA, mRNA, and glycolipids. PIM levels directly correlated with enhanced receptor-ligand signaling in vivo. Inflammatory \"keratokines\" targeting immune cells were synergistically upregulated in lipopolysaccharide-challenged HOKs, while endogenous lipoteichoic acid (LTA) correlated with CXCL1/8 expression in vitro and in vivo. Application of Drug2Cell suggested altered drug efficacy predictions based on PIM detection-agnostic of disease state. CXCL1/8 expression again correlated with LTA in epithelial cells of the nasal cavity, oropharynx, and trachea. Thus, PIMs shape epithelial single-cell profiles across upper airway mucosae.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"100"},"PeriodicalIF":7.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0