npj Biofilms and Microbiomes最新文献_第4页

Novel anti-inflammatory properties of mannose oligosaccharides in the treatment of inflammatory bowel disease via LGALS3 modulation.

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-02-08 DOI: 10.1038/s41522-025-00648-3

Yaqi Du, Yan Fan, Xin Li, Fenqin Chen

{"title":"Novel anti-inflammatory properties of mannose oligosaccharides in the treatment of inflammatory bowel disease via LGALS3 modulation.","authors":"Yaqi Du, Yan Fan, Xin Li, Fenqin Chen","doi":"10.1038/s41522-025-00648-3","DOIUrl":"10.1038/s41522-025-00648-3","url":null,"abstract":"This study investigates the role of Gum Arabic Mannose Oligosaccharides (GA-MOS) in modulating gut microbiota and alleviating symptoms of Inflammatory Bowel Disease (IBD). Employing both in vitro and in vivo models, we explored how GA-MOS influences microbial communities, particularly focusing on their capacity to enhance health-associated bacteria and reduce pathogenic species within the gut environment. Our findings reveal that GA-MOS treatment significantly altered the gut microbiota composition, increasing the abundance of anti-inflammatory bacteria while decreasing pro-inflammatory species, thus contributing to a reduction in gut inflammation and an improvement in intestinal barrier function. Detailed molecular analyses further demonstrated that these changes in microbiota were associated with modifications in the host's immune response, particularly through the suppression of key inflammatory pathways and cytokines involved in IBD progression. These results underscore the potential of dietary polysaccharides like GA-MOS as therapeutic agents in managing dysbiosis and inflammatory conditions in the gut, offering a promising approach for enhancing microbial health and overall disease management in IBD. This study provides novel insights into the bioactive properties of MOS and their interactions with gut microbiota, suggesting broader implications for their use in microbiome-centered therapies.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"26"},"PeriodicalIF":7.8,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Personalized prediction of glycemic responses to food in women with diet-treated gestational diabetes: the role of the gut microbiota.

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-02-07 DOI: 10.1038/s41522-025-00650-9

Polina V Popova, Artem O Isakov, Anastasiia N Rusanova, Stanislav I Sitkin, Anna D Anopova, Elena A Vasukova, Alexandra S Tkachuk, Irina S Nemikina, Elizaveta A Stepanova, Angelina I Eriskovskaya, Ekaterina A Stepanova, Evgenii A Pustozerov, Maria A Kokina, Elena Y Vasilieva, Lyudmila B Vasilyeva, Soha Zgairy, Elad Rubin, Carmel Even, Sondra Turjeman, Tatiana M Pervunina, Elena N Grineva, Omry Koren, Evgeny V Shlyakhto

{"title":"Personalized prediction of glycemic responses to food in women with diet-treated gestational diabetes: the role of the gut microbiota.","authors":"Polina V Popova, Artem O Isakov, Anastasiia N Rusanova, Stanislav I Sitkin, Anna D Anopova, Elena A Vasukova, Alexandra S Tkachuk, Irina S Nemikina, Elizaveta A Stepanova, Angelina I Eriskovskaya, Ekaterina A Stepanova, Evgenii A Pustozerov, Maria A Kokina, Elena Y Vasilieva, Lyudmila B Vasilyeva, Soha Zgairy, Elad Rubin, Carmel Even, Sondra Turjeman, Tatiana M Pervunina, Elena N Grineva, Omry Koren, Evgeny V Shlyakhto","doi":"10.1038/s41522-025-00650-9","DOIUrl":"10.1038/s41522-025-00650-9","url":null,"abstract":"We developed a prediction model for postprandial glycemic response (PPGR) in pregnant women, including those with diet-treated gestational diabetes mellitus (GDM) and healthy women, and explored the role of gut microbiota in improving prediction accuracy. The study involved 105 pregnant women (77 with GDM, 28 healthy), who underwent continuous glucose monitoring (CGM) for 7 days, provided food diaries, and gave stool samples for microbiome analysis. Machine learning models were created using CGM data, meal content, lifestyle factors, biochemical parameters, and microbiota data (16S rRNA gene sequence analysis). Adding microbiome data increased the explained variance in peak glycemic levels (GLUmax) from 34 to 42% and in incremental area under the glycemic curve (iAUC120) from 50 to 52%. The final model showed better correlation with measured PPGRs than one based only on carbohydrate count (r = 0.72 vs. r = 0.51 for iAUC120). Although microbiome features were important, their contribution to model performance was modest.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"25"},"PeriodicalIF":7.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population-level gut microbiome and its associations with environmental factors and metabolic disorders in Southwest China. 中国西南地区人群肠道微生物组及其与环境因素和代谢紊乱的关系。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-02-04 DOI: 10.1038/s41522-025-00661-6

Qianyu Qu, Qingyu Dou, Zhejun Xiang, Bin Yu, Lili Chen, Zhenxin Fan, Xing Zhao, Shujuan Yang, Peibin Zeng

引用次数: 0

Soft X-ray tomography reveals variations in B. subtilis biofilm structure upon tasA deletion.

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-02-02 DOI: 10.1038/s41522-025-00659-0

Anthoula Chatzimpinou, Anne Diehl, A Tobias Harhoff, Kristina Driller, Bieke Vanslembrouck, Jian-Hua Chen, Kristaps Kairišs, Valentina Loconte, Mark A Le Gros, Carolyn Larabell, Kürşad Turgay, Hartmut Oschkinat, Venera Weinhardt

{"title":"Soft X-ray tomography reveals variations in B. subtilis biofilm structure upon tasA deletion.","authors":"Anthoula Chatzimpinou, Anne Diehl, A Tobias Harhoff, Kristina Driller, Bieke Vanslembrouck, Jian-Hua Chen, Kristaps Kairišs, Valentina Loconte, Mark A Le Gros, Carolyn Larabell, Kürşad Turgay, Hartmut Oschkinat, Venera Weinhardt","doi":"10.1038/s41522-025-00659-0","DOIUrl":"10.1038/s41522-025-00659-0","url":null,"abstract":"Bacterial biofilms are complex cell communities within a self-produced extracellular matrix, crucial in various fields but challenging to analyze in 3D. We developed a \"biofilm-in-capillary\" growth method compatible with full-rotation soft X-ray tomography, enabling high-resolution 3D imaging of bacterial cells and their matrix during biofilm formation. This approach offers 50 nm isotropic spatial resolution, rapid imaging, and quantitative native analysis of biofilm structure. Using Bacillus subtilis biofilms, we detected coherent alignment and chaining of wild-type cells towards the oxygen-rich capillary tip. In contrast, the ΔtasA genetic knock-out showed a loss of cellular orientation and changes in the extracellular matrix. Adding TasA protein to the ΔtasA strain restored matrix density and led to cell assembly compaction, but without the chaining observed in wild-type biofilms. This scalable and transferable approach opens new avenues for examining biofilm structure and function across various species, including mixed biofilms, and response to genetic and environmental factors.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"23"},"PeriodicalIF":7.8,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Bifidobacterium animalis Probio-M8 improves sarcopenia physical performance by mitigating creatine restrictions imposed by microbial metabolites.

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-01-30 DOI: 10.1038/s41522-025-00656-3

Zeng Zhang, Yajing Fang, Yangli He, Mohamed A Farag, Min Zeng, Yukai Sun, Siqi Peng, Shuaiming Jiang, Xian Zhang, Kaining Chen, Meng Xu, Zhe Han, Jiachao Zhang

引用次数: 0

Time-resolved compositional and dynamics analysis of biofilm maturation and dispersal via solid-state NMR spectroscopy.

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-01-29 DOI: 10.1038/s41522-025-00655-4

Yi Xue, Xue Kang

{"title":"Time-resolved compositional and dynamics analysis of biofilm maturation and dispersal via solid-state NMR spectroscopy.","authors":"Yi Xue, Xue Kang","doi":"10.1038/s41522-025-00655-4","DOIUrl":"10.1038/s41522-025-00655-4","url":null,"abstract":"Dispersal plays a crucial role in the development and ecology of biofilms. While extensive studies focused on elucidating the molecular mechanisms governing this process, few have characterized the associated temporal changes in composition and structure. Here, we employed solid-state nuclear magnetic resonance (NMR) techniques to achieve time-resolved characterization of Bacillus subtilis biofilms over a 5-day period. The mature biofilm, established within 48 h, undergoes significant degradation in following 72 h. The steepest decline of proteins precedes that of exopolysaccharides, likely reflecting their distinct spatial distribution. Exopolysaccharide sugar units display clustered temporal patterns, suggesting the presence of distinct polysaccharide types. A sharp rise in aliphatic carbon signals on day 4 probably corresponds to a surge in biosurfactant production. Different dynamic regimes respond differently to dispersal: the mobile domain exhibits increased rigidity, while the rigid domain remains stable. These findings provide novel insights and perspectives on the complex process of biofilm dispersal.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"21"},"PeriodicalIF":7.8,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11779841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacodynamics of interspecies interactions in polymicrobial infections. 多种微生物感染中种间相互作用的药效学研究。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-01-21 DOI: 10.1038/s41522-024-00621-6

C Herzberg, J G C van Hasselt

{"title":"Pharmacodynamics of interspecies interactions in polymicrobial infections.","authors":"C Herzberg, J G C van Hasselt","doi":"10.1038/s41522-024-00621-6","DOIUrl":"10.1038/s41522-024-00621-6","url":null,"abstract":"The pharmacodynamic response of bacterial pathogens to antibiotics can be influenced by interactions with other bacterial species in polymicrobial infections (PMIs). Understanding the complex eco-evolutionary dynamics of PMIs and their impact on antimicrobial treatment response represents a step towards developing improved treatment strategies for PMIs. Here, we investigated how interspecies interactions in a multi-species bacterial community affect the pharmacodynamic response to antimicrobial treatment. To this end, we developed an in silico model which combined agent-based modeling with ordinary differential equations. Our analyses suggest that both interspecies interactions, modifying either drug sensitivity or bacterial growth rate, and drug-specific pharmacological properties drive the bacterial pharmacodynamic response. Furthermore, lifestyle of the bacterial population and the range of interactions can influence the impact of species interactions. In conclusion, this study provides a foundation for the design of antimicrobial treatment strategies for PMIs which leverage the effects of interspecies interactions.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"20"},"PeriodicalIF":7.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus abundance and oral site tropism in humans and non-human primates reflects host and lifestyle differences. 人类和非人类灵长类动物的链球菌丰度和口腔向性反映了宿主和生活方式的差异。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-01-17 DOI: 10.1038/s41522-024-00642-1

Irina M Velsko, Christina Warinner

引用次数: 0

A human oral commensal-mediated protection against Sjögren's syndrome with maintenance of T cell immune homeostasis and improved oral microbiota. 通过维持T细胞免疫稳态和改善口腔微生物群，人类口腔共通介导的对Sjögren综合征的保护作用。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-01-16 DOI: 10.1038/s41522-025-00654-5

Yu-Chao Tseng, Kai-Sheng Liao, Wei-Ting Lin, Chin Li, Chia-Bin Chang, Jie-Wei Hsu, Chin-Pui Chan, Chun-Ming Chen, Hon-Pin Wang, Hsiu-Chuan Chien, Jann-Tay Wang, Song-Chou Hsieh, Shu-Fen Wu

{"title":"A human oral commensal-mediated protection against Sjögren's syndrome with maintenance of T cell immune homeostasis and improved oral microbiota.","authors":"Yu-Chao Tseng, Kai-Sheng Liao, Wei-Ting Lin, Chin Li, Chia-Bin Chang, Jie-Wei Hsu, Chin-Pui Chan, Chun-Ming Chen, Hon-Pin Wang, Hsiu-Chuan Chien, Jann-Tay Wang, Song-Chou Hsieh, Shu-Fen Wu","doi":"10.1038/s41522-025-00654-5","DOIUrl":"10.1038/s41522-025-00654-5","url":null,"abstract":"Sjögren's syndrome (SS) is a prevalent systemic autoimmune disease with substantial impacts on women's health worldwide. Although oral Haemophilus parainfluenzae is reduced in SS, its significance remains unclear. This study aimed to elucidate the pathophysiological role of H. parainfluenzae in SS. Reduced salivary H. parainfluenzae levels in SS patients were confirmed through quantitative PCR. Oral H. parainfluenzae inoculation in NOD mice alleviated focal sialadenitis, improved salivary function, and reduced IFN-γ+CD3+ and IFN-γ+CD8+ T cells in salivary gland-draining lymph nodes, maintaining immune homeostasis against a biased type 1 response. Inoculation also enhanced salivary microbiota diversity, balanced the Firmicutes-to-Proteobacteria ratio, and reduced the overwhelming presence of Pseudomonas mendocina. In vitro, H. parainfluenzae-preconditioned A253 cells limited CD8 T cell expansion with reduced IFN-γ production. These findings suggest that H. parainfluenzae improves oral microbial diversity, promotes homeostatic T-cell immunity, and protects against SS, supporting its potential as a next-generation probiotic.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"18"},"PeriodicalIF":7.8,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of fluid friction in streamer formation and biofilm growth. 流体摩擦在流线形成和生物膜生长中的作用。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-01-15 DOI: 10.1038/s41522-024-00633-2

Cornelius Wittig, Michael Wagner, Romain Vallon, Thomas Crouzier, Wouter van der Wijngaart, Harald Horn, Shervin Bagheri

{"title":"The role of fluid friction in streamer formation and biofilm growth.","authors":"Cornelius Wittig, Michael Wagner, Romain Vallon, Thomas Crouzier, Wouter van der Wijngaart, Harald Horn, Shervin Bagheri","doi":"10.1038/s41522-024-00633-2","DOIUrl":"10.1038/s41522-024-00633-2","url":null,"abstract":"Biofilms constitute one of the most common forms of living matter, playing an increasingly important role in technology, health, and ecology. While it is well established that biofilm growth and morphology are highly dependent on the external flow environment, the precise role of fluid friction has remained elusive. We grew Bacillus subtilis biofilms on flat surfaces of a channel in a laminar flow at wall shear stresses spanning one order of magnitude (τw = 0.068 Pa to τw = 0.67 Pa). By monitoring the three-dimensional distribution of biofilm over seven days, we found that the biofilms consist of smaller microcolonies, shaped like leaning pillars, many of which feature a streamer in the form of a thin filament that originates near the tip of the pillar. While the shape, size, and distribution of these microcolonies depend on the imposed shear stress, the same structural features appear consistently for all shear stress values. The formation of streamers occurs after the development of a base structure, suggesting that the latter induces a secondary flow that triggers streamer formation. Moreover, we observed that the biofilm volume grows approximately linearly over seven days for all shear stress values, with a growth rate inversely proportional to the wall shear stress. We develop a scaling model, providing insight into the mechanisms by which friction limits biofilm growth.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"17"},"PeriodicalIF":7.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0