npj Biofilms and Microbiomes最新文献_第4页

Plasticity of the gut microbiome of golden snub-nosed monkeys (Rhinopithecus roxellana) in response to seasonal variation in diet. 金金丝猴肠道微生物群对季节饮食变化的可塑性

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-22 DOI: 10.1038/s41522-025-00806-7

Mingyi Zhang, Xiaochen Wang, Hui Yao, Ying Shen, Yang Teng, Paul A Garber, Huijuan Pan, Ming Li

{"title":"Plasticity of the gut microbiome of golden snub-nosed monkeys (Rhinopithecus roxellana) in response to seasonal variation in diet.","authors":"Mingyi Zhang, Xiaochen Wang, Hui Yao, Ying Shen, Yang Teng, Paul A Garber, Huijuan Pan, Ming Li","doi":"10.1038/s41522-025-00806-7","DOIUrl":"https://doi.org/10.1038/s41522-025-00806-7","url":null,"abstract":"The effects of seasonal fluctuations in food availability on gut microbiome composition, diversity, and function present significant challenges to animals with hard-to-digest diets. Here, we investigate seasonal variation the gut microbiome of wild golden snub-nosed monkeys (Rhinopithecus roxellana), a foregut fermenting primate, using metagenomics and metatranscriptomics data. We reconstructed 578 metagenome-assembled genomes (MAGs), 76.5% of which did not have exact matches in reference databases, highlighting the novelty of their gut microbiota. The gut microbiome of wild golden snub-nosed monkeys exhibited high diversity and enrichment in plant secondary compound metabolism during summer, while in winter it was enriched with enzymes that function in lichen polysaccharide degradation and Lachnospiraceae, which is important for energy balance. Captive monkeys on a consistent diet showed minimal seasonal variation in gut microbiome composition. Habitat changes also affected golden snub-nosed monkey microbiota community assembly and carbon cycling pathways. These findings underscore the gut microbiome's plasticity in meeting host dietary needs under varying environmental conditions.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"169"},"PeriodicalIF":9.2,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12373803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deconjugating taurocholic acid with Bifidobacterium to mitigate obesity-driven cancer progression by restoring CD8⁺ T-cell infiltration. 双歧杆菌解配牛磺胆酸可通过恢复CD8+ t细胞浸润减轻肥胖驱动的癌症进展。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-21 DOI: 10.1038/s41522-025-00809-4

Ruoshui Yuan, Yuke Li, Yifei Wang, Qingxiang Li, Chuanbin Guo, Lin Wang

{"title":"Deconjugating taurocholic acid with Bifidobacterium to mitigate obesity-driven cancer progression by restoring CD8+ T-cell infiltration.","authors":"Ruoshui Yuan, Yuke Li, Yifei Wang, Qingxiang Li, Chuanbin Guo, Lin Wang","doi":"10.1038/s41522-025-00809-4","DOIUrl":"https://doi.org/10.1038/s41522-025-00809-4","url":null,"abstract":"Obesity is linked to an increased cancer risk, and probiotics show promise in weight management. Here, we elucidate the precise mechanisms through which the probiotic Bifidobacterium breve (B. breve) modulates the immune response in obesity-associated tumours utilizing a Hepa1-6 cell-bearing hepatocellular carcinoma (HCC) model sensitive to high-fat diet (HFD)-induced obesity. HFD-induced obesity expedited HCC progression and fostered an immunosuppressive microenvironment. Treatment with B. breve enhanced cancer control by rescuing the local infiltration of antitumour immune cells. Elevated serum taurocholic acid (TCA) levels were negatively correlated with B. breve levels in obese HCC mice. TCA hindered the infiltration of CD8+ T cells into the tumour microenvironment and diminished their antitumour efficacy by blocking ERK phosphorylation. B. breve deconjugated TCA via its type 4 bile salt hydrolase (BSH), and this effect was diminished upon BSH inhibition by AAA-10. These results highlight the potential application of the probiotic B. breve in the multidisciplinary treatment of cancer in obese individuals.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"167"},"PeriodicalIF":9.2,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12370907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144963189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesaconic acid as a key metabolite with anti-inflammatory and anti-aging properties produced by Lactobacillus plantarum 124 from centenarian gut microbiota. 从百岁老人肠道菌群中提取植物乳杆菌124产生的具有抗炎和抗衰老作用的关键代谢物美沙康酸。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-19 DOI: 10.1038/s41522-025-00812-9

Lei Wu, Hongxin He, Tingting Liang, Guowei Du, Longyan Li, Haojie Zhong, Ying Li, Jinke Zhang, Ning Chen, Tong Jiang, Juan Yang, Jiangyan Wang, Shuo Feng, Shenghua Lu, Hui Zhao, Qihui Gu, He Gao, Guanghui Li, Wenrui Xie, Lihao Wu, Qingping Wu, Xinqiang Xie, Xingxiang He

{"title":"Mesaconic acid as a key metabolite with anti-inflammatory and anti-aging properties produced by Lactobacillus plantarum 124 from centenarian gut microbiota.","authors":"Lei Wu, Hongxin He, Tingting Liang, Guowei Du, Longyan Li, Haojie Zhong, Ying Li, Jinke Zhang, Ning Chen, Tong Jiang, Juan Yang, Jiangyan Wang, Shuo Feng, Shenghua Lu, Hui Zhao, Qihui Gu, He Gao, Guanghui Li, Wenrui Xie, Lihao Wu, Qingping Wu, Xinqiang Xie, Xingxiang He","doi":"10.1038/s41522-025-00812-9","DOIUrl":"10.1038/s41522-025-00812-9","url":null,"abstract":"The gut microbiota of centenarians plays a vital role in promoting healthy longevity. We performed a cross-sectional study of 224 people from Jiaoling, China, which is globally recognised for the longevity of its residents. Compared with younger people, centenarians showed significantly increased alpha-diversity, enrichment of the beneficial bacteria Lactobacillus, Akkermansia, and Christensenella, and increased redox capacity in the gut microbiota. Serum metabolomics of centenarians showed significant enrichment of antioxidant metabolites, including L-ascorbic acid 2-sulphate and lipoic acid. Finally, we isolated and screened a strain of Lactobacillus plantarum 124 (LP124) with a good antioxidant effect on the gut microbiota of centenarians. Animal experiments further verified that mesaconic acid from LP124 regulates the gut microbiota, is anti-inflammatory, relieves oxidative stress, maintains the intestinal barrier, and is the best-known anti-aging molecule. LP124 derived from the gut microbiota of centenarians and its metabolite mesaconic acid, have a significant positive effect on health and longevity.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"165"},"PeriodicalIF":9.2,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12365202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide abundance correlations in metaproteomics enhance taxonomic and functional analysis of the human gut microbiome. 宏蛋白质组学的肽丰度相关性增强了人类肠道微生物组的分类和功能分析。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-19 DOI: 10.1038/s41522-025-00801-y

Zhongzhi Sun, Zhibin Ning, Qing Wu, Leyuan Li, Andrew C Doxey, Daniel Figeys

{"title":"Peptide abundance correlations in metaproteomics enhance taxonomic and functional analysis of the human gut microbiome.","authors":"Zhongzhi Sun, Zhibin Ning, Qing Wu, Leyuan Li, Andrew C Doxey, Daniel Figeys","doi":"10.1038/s41522-025-00801-y","DOIUrl":"10.1038/s41522-025-00801-y","url":null,"abstract":"Mass spectrometry (MS)-based proteomics is widely used for quantitative protein profiling and protein interaction studies. However, most current research focuses on single-species proteomics, while protein interactions within complex microbiomes, composed of hundreds of bacterial species, remain largely unexplored. In this study, we analyzed peptide abundance correlations within a metaproteomics dataset derived from in vitro cultured human gut microbiomes subjected to various drug treatments. Our analysis revealed that peptides from the same protein or taxon exhibited correlated abundance changes. By using t-SNE for visualization, we generated a peptide correlation map in which peptides from the same taxon formed distinct clusters. Furthermore, peptide abundance correlations enabled genome-level taxonomic assignments for a greater number of peptides. For instance, 1880 (48.9%) of the 3845 peptides initially assigned only to the family Bacteroidaceae could now be assigned to a specific genome. In species representative genome subsets, peptide correlation networks based on taxon-normalized peptide abundance (TNPA) linked functionally related peptides and provided insights into uncharacterized proteins. Altogether, our study demonstrates that analyzing peptide abundance correlations enhances both taxonomic and functional analyses in human gut metaproteomics research.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"166"},"PeriodicalIF":9.2,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12365196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144883343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-functionality of Nocardiopsis alba B57 in biocontrol and plant growth: a metabolomic approach to agricultural sustainability. 白诺卡opsis B57在生物防治和植物生长中的双重功能：农业可持续性的代谢组学方法。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-18 DOI: 10.1038/s41522-025-00796-6

Osama Abdalla Abdelshafy Mohamad, Yong-Hong Liu, Tamer Elsamahy, Shuai Li, Rajivgandhi Govindan, Nigora Kuchkarova, Shaimaa Hatab, Yuanming Zhang, Wen-Jun Li

{"title":"Dual-functionality of Nocardiopsis alba B57 in biocontrol and plant growth: a metabolomic approach to agricultural sustainability.","authors":"Osama Abdalla Abdelshafy Mohamad, Yong-Hong Liu, Tamer Elsamahy, Shuai Li, Rajivgandhi Govindan, Nigora Kuchkarova, Shaimaa Hatab, Yuanming Zhang, Wen-Jun Li","doi":"10.1038/s41522-025-00796-6","DOIUrl":"10.1038/s41522-025-00796-6","url":null,"abstract":"The increasing incidence of fungal phytopathogens poses a significant challenge to agricultural sustainability, necessitating the development of environmental alternatives to synthetic fungicides and mitigating their ecological impact. This study explores the efficiency of Nocardiopsis alba B57 to produce secondary metabolites with antifungal and plant growth-promoting properties. Untargeted metabolomics using ultra-high-performance liquid chromatography (UPLC-MS/MS) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified key metabolites (e.g., carbapenem, menaquinone, and fumiquinazoline) in the co-culture environment with fungal pathogens. Additionally, principal component analysis and OPLS-DA differentiated monoculture and co-culture metabolic profiles, revealed carbapenem biosynthesis as a highly enriched pathway. The comprehensive metabolomics data and the statistical analysis of the identified metabolites confirmed that co-culturing of B57 and fungal strains showed upregulated metabolites (e.g., carbapenem and menaquinone). However, other metabolites (e.g., mupirocin) were downregulated and significantly suppressed. These changes in metabolic activity reflect the organism's adaptive and competitive responses during the co-culture conditions with fungal pathogens and influence plant hormone signaling (e.g., auxin and cytokinin), promoting plant growth and disease resistance. These findings underscore B57's adaptive biosynthetic response to co-culture conditions, supporting its use as a sustainable biocontrol agent and boosting crop productivity.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"164"},"PeriodicalIF":9.2,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12361581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effects of commensals and phage predation in suppressing colonization by pathogenic Vibrio parahaemolyticus. 共生菌和噬菌体捕食在抑制致病性副溶血性弧菌定植中的协同作用。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-16 DOI: 10.1038/s41522-025-00802-x

Ling Chen, Zhipeng Huang, Mathias Middelboe, Deng Deng, Yingfei Ma

{"title":"Synergistic effects of commensals and phage predation in suppressing colonization by pathogenic Vibrio parahaemolyticus.","authors":"Ling Chen, Zhipeng Huang, Mathias Middelboe, Deng Deng, Yingfei Ma","doi":"10.1038/s41522-025-00802-x","DOIUrl":"10.1038/s41522-025-00802-x","url":null,"abstract":"Colonization resistance is a fundamental mechanism by which microbiomes suppress pathogen invasion; however, the ecological and mechanistic determinants of its efficacy remain incompletely understood. Here, we constructed a defined microbial consortium and employed in vivo shrimp infection models to investigate the synergistic interaction between commensal microbes and a pathogen-specific phage in suppressing the pathogen Vibrio parahaemolyticus. Our in vitro experiment revealed that combining key taxa, particularly with phage integration, markedly enhanced pathogen exclusion. Furthermore, we demonstrated that establishing the consortium prior to pathogen exposure resulted in the irreversible suppression of pathogen proliferation, highlighting the critical importance of timing. Mechanistic analyses revealed that nutrient competition from commensals triggered prophage activation in the pathogen, thereby inhibiting its proliferation. Leveraging these insights, we rationally designed a minimalist, yet effective consortium that, when coupled with phage predation, consistently conferred robust colonization resistance in shrimp. This study delineates the core ecological principle underlying microbiota-mediated colonization resistance and establishes a tractable phage-commensal framework for pathogen control, with translational relevance in the context of rising antibiotic resistance in aquatic and potentially mammalian systems.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"163"},"PeriodicalIF":9.2,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144862364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endolysin selectively kills Gardnerella ex vivo in vaginal samples from women with bacterial vaginosis. 在患有细菌性阴道病的妇女的阴道样本中，内溶素选择性地杀死加德纳菌。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-12 DOI: 10.1038/s41522-025-00764-0

Lenka Podpera Tisakova, Timo Schwebs, Rocío Berdaguer, Marina von Freyberg, Philipp Foessleitner, Ann-Katrin Kieninger, Albina Poljak, Lorenzo Corsini, Alex Farr

{"title":"Endolysin selectively kills Gardnerella ex vivo in vaginal samples from women with bacterial vaginosis.","authors":"Lenka Podpera Tisakova, Timo Schwebs, Rocío Berdaguer, Marina von Freyberg, Philipp Foessleitner, Ann-Katrin Kieninger, Albina Poljak, Lorenzo Corsini, Alex Farr","doi":"10.1038/s41522-025-00764-0","DOIUrl":"10.1038/s41522-025-00764-0","url":null,"abstract":"Current treatments for bacterial vaginosis (BV) often result in recurrent disease. Gardnerella, a key player in BV pathogenesis, forms biofilms on vaginal epithelial cells. Recombinant endolysins have shown to specifically kill Gardnerella, but not commensal lactobacilli, in vitro. This study evaluated the pharmacodynamics of BNT331-endolysin (BNT331-EL) on vaginal samples from 49 women with BV (Nugent score ≥7, Amsel criteria, and clue cells). Whole genome sequencing confirmed BV-associated community state types IV-B and III, with Gardnerella dominating in 53% of samples and present in 86%. Ex vivo treatment with BNT331-EL reduced viable Gardnerella by ≥94% at 20-50 µg/mL over 19 h. L. iners was reduced by an average of 92% across samples, while L. crispatus proliferated where present in substantial amounts. Endolysin treatment effectively disrupted Gardnerella biofilms and reduced viable bacterial load in a time- and concentration-dependent manner. These results informed the definition of the treatment dose for a first-in-human trial with BNT331-EL.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"161"},"PeriodicalIF":9.2,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome drives age-dependent shifts in brain transcriptomic programs at the single-cell level in Drosophila. 微生物组在果蝇单细胞水平上驱动脑转录组程序的年龄依赖性转变。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-12 DOI: 10.1038/s41522-025-00781-z

Dianshu Zhao, Russel T Shiga, Zhangrong Song, Runhang Shu, Lipin Loo, Adam Chun Nin Wong

{"title":"Microbiome drives age-dependent shifts in brain transcriptomic programs at the single-cell level in Drosophila.","authors":"Dianshu Zhao, Russel T Shiga, Zhangrong Song, Runhang Shu, Lipin Loo, Adam Chun Nin Wong","doi":"10.1038/s41522-025-00781-z","DOIUrl":"10.1038/s41522-025-00781-z","url":null,"abstract":"The gut microbiome plays a critical role in brain function and the brain-gut axis, yet its cellular and molecular mechanisms remain unclear. Here, we present the first comprehensive single-cell transcriptomic atlas of brain cells from adult Drosophila melanogaster raised under axenic and microbiome-associated conditions, spanning young and old ages. Profiling 34,427 cells across 101 clusters, we annotated 56 cell types and identified cell type-specific gene signatures influenced by the microbiome. Transcriptional shifts were most pronounced in old flies, with glial cells and dopaminergic neurons among the most microbiome-responsive cell types. Differentially expressed genes (DEGs) were enriched in pathways related to mitochondrial activity, energy metabolism, and Notch signaling. We also quantified age-associated changes in the gut microbiome, observing reduced Acetobacter dominance and increased microbial diversity that corresponded with heightened brain transcriptional responses. These findings illuminate the cell type-specific impacts of the microbiome on brain gene expression and lay the groundwork for understanding the molecular underpinnings of the microbiome-gut-brain axis.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"162"},"PeriodicalIF":9.2,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An anaerobic in vitro flow model for studying interactions at the gastrointestinal host-microbe interface. 用于研究胃肠道宿主-微生物界面相互作用的体外厌氧流动模型。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-11 DOI: 10.1038/s41522-025-00800-z

L L Bang, J S Pettersen, N Høiland, A M Rojek, D R Tornby, J Møller-Jensen, U S Justesen, R M Pedersen, T E Andersen

{"title":"An anaerobic in vitro flow model for studying interactions at the gastrointestinal host-microbe interface.","authors":"L L Bang, J S Pettersen, N Høiland, A M Rojek, D R Tornby, J Møller-Jensen, U S Justesen, R M Pedersen, T E Andersen","doi":"10.1038/s41522-025-00800-z","DOIUrl":"10.1038/s41522-025-00800-z","url":null,"abstract":"In vitro research on host-microbe interactions in the human gut has been challenging due to the differing oxygen requirements of mammalian cells and intestinal microbiota. Few models of this environment have been developed, and those available are complex, limiting the extraction of important information during experiments. Here we report an in vitro model that by simple means creates an anaerobic environment for microbiota growing on living, cultured human epithelium under physiological flow. This model enables long-term co-culture of intestinal epithelial cells with obligate anaerobic bacteria, exemplified here by Clostridioides difficile and Bacteroides fragilis. Anaerobic conditions are maintained through the integration of an anaerobization unit, developed to facilitate online deoxygenation of media via liquid-to-liquid gas diffusion, eliminating the need for encapsulation in complex gas chambers. We show that stable oxygen levels of less than 1% can be maintained in the model for several days without compromising the viability of the intestinal epithelium. Furthermore, we demonstrate the performance of the model by simulating prolonged colonization with C. difficile and B. fragilis, as well as the clinically relevant persistence of C. difficile following treatment with vancomycin.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"160"},"PeriodicalIF":9.2,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12340051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144822147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study. 评估微生物群落在预测喉癌复发中的预后价值：一项多中心病例对照研究。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-10 DOI: 10.1038/s41522-025-00789-5

Chi-Yao Hsueh, Xiaohui Yuan, Huiying Huang, Yujie Shen, Qiang Huang, Weida Dong, Danhui Li, Hui-Ching Lau, Xinhui Mao, Simin Liang, Lei Tao, Ming Zhang, Ji Sun, Hongli Gong

{"title":"Evaluating the prognostic value of microbial communities in predicting recurrence of laryngeal carcinoma: a multicenter case-control study.","authors":"Chi-Yao Hsueh, Xiaohui Yuan, Huiying Huang, Yujie Shen, Qiang Huang, Weida Dong, Danhui Li, Hui-Ching Lau, Xinhui Mao, Simin Liang, Lei Tao, Ming Zhang, Ji Sun, Hongli Gong","doi":"10.1038/s41522-025-00789-5","DOIUrl":"10.1038/s41522-025-00789-5","url":null,"abstract":"Laryngeal squamous cell carcinoma (LSCC) presents significant treatment challenges, especially regarding recurrence after larynx-preservation therapy. We identified distinct microbial community structures between recurrence and non-recurrence groups, particularly highlighting the genera abundance of Fusobacterium and Serratia. However, larynx-preserving therapy did not significantly alter microbial diversity in recurrent patients. Survival analysis identified Fusobacterium and Serratia as independent prognostic factors for recurrence, leading to the development of a Serratia-Fusobacterium (SF) prognostic scoring model. The SF model achieved an AUC of 81.37% for predicting recurrence, outperforming the TNM staging system. LSCC patients classified as high-risk by the SF model exhibited significantly shorter disease-free survival (DFS) compared to low-risk patients in the LSCC cohort. Furthermore, the SF model demonstrated an AUC of 78.48% in the multi-center cohort for predicting recurrence. In conclusion, the Serratia-Fusobacterium prognostic scoring model can predict LSCC recurrence after larynx-preserving therapy and provide valuable insights to inform recommendations for LSCC surveillance.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"159"},"PeriodicalIF":9.2,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12335459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0