npj Biofilms and Microbiomes最新文献_第5页

Microbiome-mediated colonization resistance to carbapenem-resistant Klebsiella pneumoniae in ICU patients. ICU患者对碳青霉烯耐药肺炎克雷伯菌微生物介导的定植耐药性

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-09 DOI: 10.1038/s41522-025-00791-x

Jing Yang, Yi Zhou, Aiping Du, Zhongwei Zhang, Bo Wang, Yongming Tian, Huan Liu, Lin Cai, Fang Pang, Yumei Li, Chunhua Du, Xijun Wu, Cong Yan, Wei Wu, Min Jiang, Ke Shen, Chi Zhang, Yu Feng, Yan Kang, Bairong Shen, Zhiyong Zong

{"title":"Microbiome-mediated colonization resistance to carbapenem-resistant Klebsiella pneumoniae in ICU patients.","authors":"Jing Yang, Yi Zhou, Aiping Du, Zhongwei Zhang, Bo Wang, Yongming Tian, Huan Liu, Lin Cai, Fang Pang, Yumei Li, Chunhua Du, Xijun Wu, Cong Yan, Wei Wu, Min Jiang, Ke Shen, Chi Zhang, Yu Feng, Yan Kang, Bairong Shen, Zhiyong Zong","doi":"10.1038/s41522-025-00791-x","DOIUrl":"10.1038/s41522-025-00791-x","url":null,"abstract":"Carbapenem-resistant Klebsiella pneumoniae (CRKP) causes serious intensive care unit (ICU)-acquired infections, yet the mechanisms of microbiota-mediated colonization resistance remain unclear. We analyzed the gut microbiome and metabolic profiles of healthy individuals and ICU patients, distinguishing those with and without CRKP colonization. ICU patients showed distinct microbial communities compared to healthy controls, and CRKP-positive patients exhibited unique microbial and metabolic signatures. We demonstrated that a healthy gut microbiome is essential for providing resistance against CRKP colonization in antibiotic-perturbed mouse with fecal microbiota transplantation (FMT). Both in vitro and in vivo experiments revealed that Lactiplantibacillus plantarum and Bifidobacterium longum as significant contributors to the decolonization of CRKP. Furthermore, we showed that probiotic supplementation or FMT significantly improved CRKP colonization resistance. The findings highlight that a specific gut microbiome is essential for resisting CRKP colonization, and that targeted microbiome restoration may serve as a viable strategy to prevent CRKP colonization in ICU patients.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"157"},"PeriodicalIF":9.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12335454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-fat and low-fiber diet elevates the gut resistome: a comparative metagenomic study. 高脂肪和低纤维饮食提高肠道抵抗组：一项比较宏基因组研究。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-09 DOI: 10.1038/s41522-025-00799-3

Yingbo Shen, Da Sun, Kun Chen, Junyao Jiang, Dongyan Shao, Lu Yang, Chengtao Sun, Dejun Liu, Yuebin Ke, Congming Wu, Timothy R Walsh, Jianzhong Shen, Ziquan Lv, Yang Wang

{"title":"High-fat and low-fiber diet elevates the gut resistome: a comparative metagenomic study.","authors":"Yingbo Shen, Da Sun, Kun Chen, Junyao Jiang, Dongyan Shao, Lu Yang, Chengtao Sun, Dejun Liu, Yuebin Ke, Congming Wu, Timothy R Walsh, Jianzhong Shen, Ziquan Lv, Yang Wang","doi":"10.1038/s41522-025-00799-3","DOIUrl":"10.1038/s41522-025-00799-3","url":null,"abstract":"Antimicrobial resistance (AMR) is a global health challenge. The gut microbiome, a major reservoir for AMR, is influenced by dietary habits. However, the specific impact of dietary patterns on gut resistome remains poorly understood. This study aimed to assess the effects of high-fat/low-fiber and high-fiber/low-fat diets on the development of AMR in the gut microbiome. The shift from a normal diet to a high-fat/low-fiber or a high-fiber/low-fat diet in mice resulted in corresponding increases and decreases in the relative abundance of the resistome (0.14 to 0.25, p < 0.001 vs. 0.14 to 0.09 p < 0.05), virulence genes (VGs) (0.56 to 0.91, p < 0.001 vs. 0.58 to 0.50, p < 0.05), and mobile genetic elements (MGEs) (0.20 to 1.66, p < 0.001 vs. 0.22 to 0.13, p < 0.05), respectively. Network analyses identified bacteria such as Bacteroides, Parabacteroides, and Alistipes as hosts of ARGs and VGs, with changes in their abundance closely associated with shifts in ARG and VG levels. Mobile genetic elements such as Tn916, ISBf10, IS91, and intl1 were linked to these variations, including genes conferring resistance to vancomycin and capsule-related VGs. In humans, a similar trend was observed, with high-fat diets correlating with higher resistome levels, while high-fiber diets were associated with lower resistome levels compared to a normal diet group. ARGs were more prevalent in pathogenic genera such as Enterococcus spp., Klebsiella spp., Pseudomonas spp., and Staphylococcus spp. The high-fat/low-fiber diet increased the bacterial resistome and VG abundance compared to a high-fiber/low-fat diet. Therefore, adopting a high-fiber/low-fat diet may be an effective strategy to reduce the AMR burden in the human gut, providing a valuable insight for public health recommendations.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"156"},"PeriodicalIF":9.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12335491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of a multi-strain L. crispatus-based vaginal synbiotic on the vaginal microbiome: a randomized placebo-controlled trial. 一种多菌株的基于葡萄球菌的阴道合成菌对阴道微生物组的影响：一项随机安慰剂对照试验。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-09 DOI: 10.1038/s41522-025-00788-6

Jacques Ravel, Sheri Simmons, Eleni Greenwood Jaswa, Sara Gottfried, Miriam Greene, Susan Kellogg-Spadt, Dirk Gevers, Diane M Harper

引用次数: 0

Periodontitis-associated Fusobacterium nucleatum promotes ulcerative colitis by ferroptosis-mediated gut barrier disruption. 牙周炎相关的核梭杆菌通过铁中毒介导的肠道屏障破坏促进溃疡性结肠炎。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-09 DOI: 10.1038/s41522-025-00763-1

Xiaoyue Zhang, Shouzheng Cheng, Shuze Chen, Qiuhao Wang, Jeiyu Zhou, Hui Wang, Lei Cheng, Lei Zhao

{"title":"Periodontitis-associated Fusobacterium nucleatum promotes ulcerative colitis by ferroptosis-mediated gut barrier disruption.","authors":"Xiaoyue Zhang, Shouzheng Cheng, Shuze Chen, Qiuhao Wang, Jeiyu Zhou, Hui Wang, Lei Cheng, Lei Zhao","doi":"10.1038/s41522-025-00763-1","DOIUrl":"10.1038/s41522-025-00763-1","url":null,"abstract":"Periodontitis and ulcerative colitis (UC) are inflammatory diseases linked through the \"gum-gut\" axis. Fusobacterium nucleatum, an important periodontitis-associated pathobiont and gastrointestinal opportunist, may mediate their comorbidity. This study investigated the role of F. nucleatum in UC using dextran sulfate sodium-induced UC and F. nucleatum-induced periodontitis models. F. nucleatum exacerbated inflammatory alveolar bone loss and intestinal barrier dysfunction, accelerating UC severity. Integrated 16S rRNA gene sequence and LC-MS metabolomics revealed ferroptosis activation, characterized by elevated Fe2+ and malondialdehyde, glutathione depletion, dysregulated GPX4, FTH1, and ACSL4 expression, reduced mitochondrial membrane potential, and reactive oxygen species aggregation in the mouse colon and colonic epithelial cell CCD841. Administration of ferroptosis inhibitor Ferrostatin-1 attenuated UC by restoring intestinal permeability, preserving mucin layers, and enhancing tight junction proteins ZO-1 and CLDN-1. These findings establish F. nucleatum as a key mediator of periodontitis-UC comorbidity through ferroptosis-mediated gut barrier disruption, providing mechanistic insights into microbial-driven inflammatory cross-talk.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"155"},"PeriodicalIF":9.2,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12335446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mobiluncus mulieris alters the transcriptomic profile of cervicovaginal epithelial cells, shedding light on molecular drivers of adverse reproductive outcomes. Mobiluncus muleris改变了宫颈阴道上皮细胞的转录组学特征，揭示了不良生殖结果的分子驱动因素。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-08 DOI: 10.1038/s41522-025-00784-w

Yu Hasegawa, Olivia Swain, Urvija Rajpal, Michael T France, Liqhwa Ncube, Haocheng Yu, Ilaria Mogno, Amir Horowitz, Jacques Ravel, Michal A Elovitz

{"title":"Mobiluncus mulieris alters the transcriptomic profile of cervicovaginal epithelial cells, shedding light on molecular drivers of adverse reproductive outcomes.","authors":"Yu Hasegawa, Olivia Swain, Urvija Rajpal, Michael T France, Liqhwa Ncube, Haocheng Yu, Ilaria Mogno, Amir Horowitz, Jacques Ravel, Michal A Elovitz","doi":"10.1038/s41522-025-00784-w","DOIUrl":"10.1038/s41522-025-00784-w","url":null,"abstract":"The vaginal microbiota is critical for reproductive health, and its disruption, particularly the loss of Lactobacillus spp. and dominance of anaerobes such as Mobiluncus mulieris (community state type IV, CST IV), is associated with bacterial vaginosis, sexually transmitted infections, and adverse reproductive outcomes, including preterm birth (PTB). While Gardnerella spp. have been widely studied, the role of M. mulieris remains poorly understood. This study used an unbiased discovery approach to examine host-microbe interactions driven by M. mulieris across distinct epithelial barriers of the lower reproductive tract. RNA sequencing revealed that live bacteria, cell-free supernatant, and bacterial extracellular vesicles (bEVs) each induced unique transcriptional responses in epithelial cells. All three components activated immune and inflammatory pathways, with bEVs eliciting the strongest response, particularly via toll-like receptor (TLR) 2 and TLR5 signaling. M. mulieris also altered extracellular matrix (ECM) remodeling pathways, including upregulation of matrix metalloproteinase 9 (MMP9), a key mediator linked to PTB. These findings were supported by clinical data showing elevated MMP9 in pregnant women with M. mulieris-containing vaginal microbiota. Collectively, these results highlight the broad impact of M. mulieris on epithelial responses and identify mechanisms by which specific anaerobes contribute to inflammation and ECM disruption in adverse reproductive outcomes.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"154"},"PeriodicalIF":9.2,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12331918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level. 在菌株水平上肠道共生罗氏菌的基因组见解和代谢谱。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-05 DOI: 10.1038/s41522-025-00793-9

Xin-Wei Sun, Hao-Jie Huang, Yu-Zhi Zhao, Hao-Yu Chen, Chang-Yu Wang, Zheng Zhou, Yu Jiang, Run-Yu Han, He Jiang, Chang Liu, Shuang-Jiang Liu

{"title":"Genomic insights and metabolic profiling of gut commensal Luoshenia tenuis at strain level.","authors":"Xin-Wei Sun, Hao-Jie Huang, Yu-Zhi Zhao, Hao-Yu Chen, Chang-Yu Wang, Zheng Zhou, Yu Jiang, Run-Yu Han, He Jiang, Chang Liu, Shuang-Jiang Liu","doi":"10.1038/s41522-025-00793-9","DOIUrl":"10.1038/s41522-025-00793-9","url":null,"abstract":"Luoshenia tenuis, a newly identified gut commensal microbe from the family Christensenellaceae, has shown therapeutic effects on weight control and metabolic disorders in model mice. Bacterial strains are essential for investigations on the host-microbe interaction and further development of medical applications. In this study, we collected 27 strains of L. tenuis from the Christensenellaceae Gut Microbial Biobank (ChrisGMB) and sequenced their complete genomes. Our analysis revealed considerable genetic diversity and genomic plasticity. Metabolic prediction indicated that L. tenuis had a preference for metabolizing plant-derived carbohydrates and the ability to synthesize various amino acids and cofactors. In silico analysis, along with in vitro experiments, validated that L. tenuis strains possessed strong acid tolerance and limited antibiotic resistance, suitable traits for oral probiotic development. Further volatile metabolomics and bile acid transformation profiling revealed that L. tenuis was capable of producing metabolites with previously-identified beneficial effects, along with extensive bile acid modification, potentially contributing to its positive impact on host metabolism. This study provides essential insight into strain-level functional and genomic features, laying a foundation for future research towards the development of L. tenuis-based therapies for metabolic disease.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"153"},"PeriodicalIF":9.2,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12325933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes. RaS-RiPP溶菌素及培养条件对离体口腔微生物群的影响。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-04 DOI: 10.1038/s41522-025-00794-8

Britta E Rued, Achal Dhariwal, Brett C Covington, Mohammad R Seyedsayamdost, Sophie A Krivograd, Russell P Pesavento, Michael J Federle, Fernanda C Petersen

{"title":"Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes.","authors":"Britta E Rued, Achal Dhariwal, Brett C Covington, Mohammad R Seyedsayamdost, Sophie A Krivograd, Russell P Pesavento, Michael J Federle, Fernanda C Petersen","doi":"10.1038/s41522-025-00794-8","DOIUrl":"10.1038/s41522-025-00794-8","url":null,"abstract":"Natural products are ubiquitously produced by many species that we encounter during our daily lives. One genus, Streptococcus, can produce a wide array of quorum sensing linked natural products known as RaS-RiPPs (ribosomally synthesized and post-translationally modified peptides). Their production is triggered by the induction of an Rgg-SHP quorum sensing system, which senses the presence of SHPs (short hydrophobic peptides) and induces the gene expression of these operons. Previous work has found that streptococcal RaS-RiPPs modulate the growth of different streptococci and might play a role in antibiotic tolerance. This is of particular importance to the oral microbiome, where streptococci are a predominant genus. This study provides the first report on attempts to study the impact of the RaS-RiPP Tryglysin A on ex-vivo oral systems and explores important factors to consider when culturing these systems. We explore how medium selection, atmosphere, growth model, and saliva amount can impact the presence of both bacterial and fungal species. These studies provide the groundwork for determining how RaS-RiPP producing Streptococci might impact the composition and function of oral microbiome communities, as well as important aspects to consider when culturing ex-vivo oral systems.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"152"},"PeriodicalIF":9.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12322106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The adoption of a westernized gut microbiome in Indian Immigrants and Indo-Canadians is associated with dietary acculturation. 印度移民和印度裔加拿大人采用西化的肠道微生物群与饮食文化适应有关。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-03 DOI: 10.1038/s41522-025-00778-8

Leah D D'Aloisio, Mamatha Ballal, Sanjoy Ghosh, Natasha Haskey, Nijiati Abulizi, Ramin Karimianghadim, Chuyi Liu, Pacha Sruthi, Lakshmipriya Nagarajan, Sudha Vasudevan, Vignesh Shetty, Mrudgandha Purandare, Ushasi Bhaumik, Debaki Ranjan Howlader, Sepideh Pakpour, Jacqueline Barnett, Deanna L Gibson

{"title":"The adoption of a westernized gut microbiome in Indian Immigrants and Indo-Canadians is associated with dietary acculturation.","authors":"Leah D D'Aloisio, Mamatha Ballal, Sanjoy Ghosh, Natasha Haskey, Nijiati Abulizi, Ramin Karimianghadim, Chuyi Liu, Pacha Sruthi, Lakshmipriya Nagarajan, Sudha Vasudevan, Vignesh Shetty, Mrudgandha Purandare, Ushasi Bhaumik, Debaki Ranjan Howlader, Sepideh Pakpour, Jacqueline Barnett, Deanna L Gibson","doi":"10.1038/s41522-025-00778-8","DOIUrl":"10.1038/s41522-025-00778-8","url":null,"abstract":"Indian immigration to westernized countries has recently surged, increasing their risk of Inflammatory Bowel Disease (IBD) post-migration. While crucial for understanding IBD risk, the gut microbiome remains understudied in Indians. This cross-sectional study examines the impact of westernization on the gut microbiomes of Indians residing in India, Indo-Immigrants, and Indo-Canadians compared to Euro-Canadian and Euro-Immigrant controls. Stool samples for 16S rRNA and shotgun sequencing assessed microbial taxa and functional profiles, alongside dietary and demographic data to evaluate lifestyle patterns. Indians and Indo-Immigrants had distinct microbiotas from controls, with high abundances of Prevotella spp. and CAZymes reflecting their high complex carbohydrate diet. Indo-Canadians exhibited a transitional microbiome towards westernization, mirroring increasing dietary acculturation. Considering 44% of Canadians are first- and second-generation immigrants and the global adoption of westernized practices, future research should investigate the health implications of such microbiome transitions in immigrant populations and newly industrialized nations.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"151"},"PeriodicalIF":9.2,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12317999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut-resident Phascolarctobacterium succinatutens decreases fat accumulation via MYC-driven epigenetic regulation of arginine biosynthesis. 通过myc驱动的精氨酸生物合成的表观遗传调控，肠道内的琥珀酸相乳杆菌减少脂肪积累。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-02 DOI: 10.1038/s41522-025-00792-w

Qun Lan, Sui Liufu, Bohe Chen, Kaiming Wang, Wenwu Chen, Lanlin Xiao, Xiaolin Liu, Lei Yi, Jingwen Liu, Xin Xu, Caihong Liu, Mei Liu, Yulong Yin, Haiming Ma

{"title":"Gut-resident Phascolarctobacterium succinatutens decreases fat accumulation via MYC-driven epigenetic regulation of arginine biosynthesis.","authors":"Qun Lan, Sui Liufu, Bohe Chen, Kaiming Wang, Wenwu Chen, Lanlin Xiao, Xiaolin Liu, Lei Yi, Jingwen Liu, Xin Xu, Caihong Liu, Mei Liu, Yulong Yin, Haiming Ma","doi":"10.1038/s41522-025-00792-w","DOIUrl":"10.1038/s41522-025-00792-w","url":null,"abstract":"Uncovering the mechanisms of excessive fat accumulation in livestock can not only protect animal health but also maintain the revenue of the intensive feeding industry. In this study, a bacteria-wide association study was conducted in a cohort of 129 commercial Yorkshire pigs. We found that Phascolarctobacterium succinatutens (P. succinatutens) was a key bacterium with greater abundance in low backfat thickness (LBF) pigs and was positively correlated with serum arginine concentrations. Fecal microbiota transplantation (FMT) experiment verified the beneficial roles of P. succinatutens in intestinal and lipid homeostasis. Administration of P. succinatutens in mice curbed weight gain, reduced adipocyte size, attenuated gut inflammation aggravation, and elevated circulating arginine levels. Propionate, a main metabolite produced by P. succinatutens, played a significant role in the above effects. Mechanistically, we indicated that P. succinatutens-generated propionate alleviated colonic inflammation by inhibiting the TLR4 signaling cascade. Importantly, propionate was found to stimulate the de novo synthesis of arginine by inhibiting the chromatin accessibility of MYC near the intron region. Finally, we found that the increase of arginine induced by P. succinatutens reduced fat deposition by suppressing the PI3K/Akt/FOXO3a signaling pathway. Our work provides novel insights into the epigenetic regulation of probiotic-mediated anti-obesity effects and highlights the potential of P. succinatutens in combating excessive obesity in commercial pigs.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"150"},"PeriodicalIF":9.2,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12318001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infant gut microbiota and SCFAs mediate the association between early-life human milk microbiota and neurodevelopment. 婴儿肠道微生物群和SCFAs介导早期母乳微生物群与神经发育之间的关联。

IF 9.2 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-08-01 DOI: 10.1038/s41522-025-00790-y

Simou Wu, Wen Jia, Jinxing Li, Yating Luo, Fei Chen, Ting Yang, Xia Jiang, Fang He, Ruyue Cheng

{"title":"Infant gut microbiota and SCFAs mediate the association between early-life human milk microbiota and neurodevelopment.","authors":"Simou Wu, Wen Jia, Jinxing Li, Yating Luo, Fei Chen, Ting Yang, Xia Jiang, Fang He, Ruyue Cheng","doi":"10.1038/s41522-025-00790-y","DOIUrl":"10.1038/s41522-025-00790-y","url":null,"abstract":"Human milk microbiota (HMM) plays a key role in infant gut microbiota (IGM) establishment, however, the influence of the early life HMM in later neurodevelopment remains unclear. In this mother-infant cohort, we investigated HMM development and its impact on IGM-neurodevelopment crosstalk. Breast milk and infant feces were collected on days 0, 7, and 30 for sequencing and SCFAs quantification, while neurodevelopment was assessed via ASQ-3 at 12 months. HMM remained stable during the first month, while IGM fluctuated significantly within the first 7 days. Songbird and mediation analyses revealed strengthening associations between HMM, IGM, and neurodevelopment over time. IGM served as a key mediator linking HMM to neurodevelopment, with SCFAs playing a mediating role in the connection between IGM and neurodevelopment on day 30. Our findings suggest that early-life stable and unique HMM may influence long-term neurodevelopment by dynamically modulating IGM and SCFAs, highlighting a potential strategy to prevent neurodysplasia by monitoring mother-infant microecology.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"149"},"PeriodicalIF":9.2,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12316973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0