npj Biofilms and Microbiomes最新文献_第5页

Akkermansia muciniphila ameliorates doxorubicin-induced cardiotoxicity by regulating PPARα-dependent mitochondrial biogenesis. 嗜muciniphila通过调节ppar α依赖的线粒体生物发生改善阿霉素诱导的心脏毒性。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-23 DOI: 10.1038/s41522-025-00712-y

Hui Lin, Xian Shao, Haodi Gu, Xinrou Yu, Lingyan He, Jiedong Zhou, Zuoquan Zhong, Shitian Guo, Dan Li, Fei Chen, Yongfei Song, Lili Xu, Ping Wang, Liping Meng, Jufang Chi, Jiangfang Lian

{"title":"Akkermansia muciniphila ameliorates doxorubicin-induced cardiotoxicity by regulating PPARα-dependent mitochondrial biogenesis.","authors":"Hui Lin, Xian Shao, Haodi Gu, Xinrou Yu, Lingyan He, Jiedong Zhou, Zuoquan Zhong, Shitian Guo, Dan Li, Fei Chen, Yongfei Song, Lili Xu, Ping Wang, Liping Meng, Jufang Chi, Jiangfang Lian","doi":"10.1038/s41522-025-00712-y","DOIUrl":"10.1038/s41522-025-00712-y","url":null,"abstract":"Doxorubicin (DOX) is a key chemotherapeutic agent but is also a leading cause of DOX-induced cardiotoxicity (DIC), limiting its clinical use. Akkermansia muciniphila (A. muciniphila), known for its benefits as a probiotic in treating metabolic syndrome, has uncertain effects in the context of DIC. Here, 16S rRNA sequencing of fecal samples from anthracycline-treated patients and DIC mice revealed marked depletion of A. muciniphila. Cardiac transcriptomics, supported by in vitro experiments, showed that A. muciniphila colonization improved mitochondrial function and alleviated DIC by activating the PPARα/PGC1α signaling pathway in both normal and antibiotic-treated C57BL/6 mice. Further analysis uncovered a restructured microbiome-metabolome network following A. muciniphila administration, which contributed to DIC protection. Notably, A. muciniphila supplementation increased serum levels of the tryptophan metabolite indole-3-propionic acid (IPA), which binds to the cardiac aryl hydrocarbon receptor (AhR), leading to the activation of the PPARα/PGC1α signaling pathway. In conclusion, our study sheds light on the potential of A. muciniphila as a probiotic in mitigating DIC.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"86"},"PeriodicalIF":7.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Navigating complexities of polymorphic microbiomes in endometrial cancer. 子宫内膜癌中多态微生物群的导航复杂性。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-22 DOI: 10.1038/s41522-025-00690-1

Nicole R Jimenez, Chloe R Herman, Paweł Łaniewski, Emily Cope, Keehoon Lee, Nichole D Mahnert, Dana M Chase, J Gregory Caporaso, Melissa M Herbst-Kralovetz

{"title":"Navigating complexities of polymorphic microbiomes in endometrial cancer.","authors":"Nicole R Jimenez, Chloe R Herman, Paweł Łaniewski, Emily Cope, Keehoon Lee, Nichole D Mahnert, Dana M Chase, J Gregory Caporaso, Melissa M Herbst-Kralovetz","doi":"10.1038/s41522-025-00690-1","DOIUrl":"10.1038/s41522-025-00690-1","url":null,"abstract":"The microbiome is key to understanding endometrial cancer (EC) etiology and prevention strategies, implicated in the regulation of estrogen in estrogen-driven cancers. Utilizing robust methodologies in the QIIME 2 platform, we examined 16S rRNA vaginal and rectal microbiome data from an EC cohort: 192 women with benign gynecologic conditions, endometrial hyperplasia, or endometrial cancer. Distinct microbial compositions and community networks specific to EC were identified and related to histological grade with adjustments for EC risk factors. Vaginal health-associated Lactobacillus and Limosilactobacillus, and rectal Prevotella and Peptoniphilus, were depleted in EC, while detrimental vaginal Anaerococcus, Porphyromonas, Prevotella, Peptoniphilus, and rectal Buttiaxella were enriched. Significant bacterial features were shared between rectal and vaginal sites in EC, such as Prevotella timonensis and Peptoniphilus A. Vaginal Lactobacillus abundance contributed to less feature sharing from the rectum. Putative microbial metabolic analysis identified dysregulation of amino acid, complex carbohydrate, and hormone metabolism amongst patients with EC.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"85"},"PeriodicalIF":7.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenicity of commensal gut biofilm in prefrail aging. 共生肠道生物膜在衰老前期的致病性。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-22 DOI: 10.1038/s41522-025-00716-8

Guillaume Le Cosquer, Melissa Pannier, Elodie Meunier, Julie Thevenin, Elise Pyhourquet, Sophie Guyonnet, Bruno Vellas, Yohan Santin, Bruno Guiard, Angelo Parini, Louis Buscail, Barbara Bournet, Damien Guillemet, Celine Deraison, Nathalie Vergnolle, Jean-Paul Motta

{"title":"Pathogenicity of commensal gut biofilm in prefrail aging.","authors":"Guillaume Le Cosquer, Melissa Pannier, Elodie Meunier, Julie Thevenin, Elise Pyhourquet, Sophie Guyonnet, Bruno Vellas, Yohan Santin, Bruno Guiard, Angelo Parini, Louis Buscail, Barbara Bournet, Damien Guillemet, Celine Deraison, Nathalie Vergnolle, Jean-Paul Motta","doi":"10.1038/s41522-025-00716-8","DOIUrl":"10.1038/s41522-025-00716-8","url":null,"abstract":"Pathophysiological mechanisms of unhealthy aging, particularly the transition from robustness to frailty, remain poorly understood. Despite extensive microbiome research on taxonomy, the behavior of early prefrail gut bacteria in their natural community-host mucosal tissue context remains unexplored. Using fecal samples from the INSPIRE-T aging human cohort, we characterized gut microbiota phenotype during prefrailty stages using a polymicrobial biofilm model. Results revealed that prefrail-derived biofilms exhibited distinct taxonomic and physical alterations, enhanced dispersal, and increased epithelial virulence compared to robust counterparts. Multiparametric analyses linked biofilm characteristics to clinical traits, suggesting their potential as aging status indicators. Polyphenol-rich grape pomace extract partially reversed prefrail biofilm alterations and reduced proinflammatory prefrail biofilm responses in vitro. Microbiota from prefrail-aged mice induced colon damage in antibiotic-treated recipients, establishing a prefrail microbiome-inflammation causality. Overall, the findings identified novel prefrail microbiome characteristics, established causal inflammatory links, and supported microbiota-targeted geroprotective interventions for the prefrail populations.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"84"},"PeriodicalIF":7.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A polymicrobial perspective into the ecological role of Enterococcus faecalis in dental root canal infections. 粪肠球菌在牙根管感染中的生态学作用的多微生物视角。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-22 DOI: 10.1038/s41522-025-00722-w

Ana Parga, Jade Mattu, Georgios N Belibasakis, Kimberly A Kline, Julian G Leprince, Daniel Manoil

引用次数: 0

Bacteriophage-driven microbial phenotypic heterogeneity: ecological and biogeochemical importance. 噬菌体驱动的微生物表型异质性：生态和生物地球化学的重要性。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-21 DOI: 10.1038/s41522-025-00727-5

Xiaolong Liang, Shuo Yang, Mark Radosevich, Yongfeng Wang, Ning Duan, Yongfeng Jia

引用次数: 0

Fusobacterium nucleatum is enriched in invasive biofilms in colorectal cancer. 结直肠癌浸润性生物膜中富含核梭杆菌。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-20 DOI: 10.1038/s41522-025-00717-7

Jessica Queen, Zam Cing, Hana Minsky, Asmita Nandi, Taylor Southward, Jacqueline Ferri, Madison McMann, Thevambiga Iyadorai, Jamuna Vadivelu, April Roslani, Mun Fai Loke, Jane Wanyiri, James R White, Julia L Drewes, Cynthia L Sears

{"title":"Fusobacterium nucleatum is enriched in invasive biofilms in colorectal cancer.","authors":"Jessica Queen, Zam Cing, Hana Minsky, Asmita Nandi, Taylor Southward, Jacqueline Ferri, Madison McMann, Thevambiga Iyadorai, Jamuna Vadivelu, April Roslani, Mun Fai Loke, Jane Wanyiri, James R White, Julia L Drewes, Cynthia L Sears","doi":"10.1038/s41522-025-00717-7","DOIUrl":"10.1038/s41522-025-00717-7","url":null,"abstract":"Fusobacterium nucleatum is an oral bacterium known to colonize colorectal tumors, where it is thought to play an important role in cancer progression. Recent advances in sequencing and phenotyping of F. nucleatum have revealed important differences at the subspecies level, but whether these differences impact the overall tumor ecology, and tumorigenesis itself, remain poorly understood. In this study, we sought to characterize Fusobacteria in the tumor microbiome of a cohort of individuals with CRC through a combination of molecular, spatial, and microbiologic analyses. We assessed for relative abundance of F. nucleatum in tumors compared to paired normal tissue, and correlated abundance with clinical and pathological features. We demonstrate striking enrichment of F. nucleatum and the recently discovered subspecies animalis clade 2 (Fna C2) specifically in colon tumors that have biofilms, highlighting the importance of complex community partnerships in the pathogenesis of this important organism.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"81"},"PeriodicalIF":7.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dental biofilms contain DNase I-resistant Z-DNA and G-quadruplexes but alternative DNase overcomes this resistance. 牙齿生物膜含有耐dna酶i的Z-DNA和g -四联体，但替代dna酶克服了这种抗性。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-19 DOI: 10.1038/s41522-025-00694-x

Dominique C S Evans, Mathilde F Kristensen, Gabriel Antonio S Minero, Lorena G Palmén, Inge Knap, Manish K Tiwari, Sebastian Schlafer, Rikke L Meyer

{"title":"Dental biofilms contain DNase I-resistant Z-DNA and G-quadruplexes but alternative DNase overcomes this resistance.","authors":"Dominique C S Evans, Mathilde F Kristensen, Gabriel Antonio S Minero, Lorena G Palmén, Inge Knap, Manish K Tiwari, Sebastian Schlafer, Rikke L Meyer","doi":"10.1038/s41522-025-00694-x","DOIUrl":"10.1038/s41522-025-00694-x","url":null,"abstract":"Extracellular DNA (eDNA) in bacterial biofilms can form non-canonical structures like Z-DNA and G-quadruplex (G4), which enhance biofilm resilience by providing protection against mammalian DNases. However, the conformation of eDNA in dental biofilms remains unexplored. Using fluorescence immunolabeling and confocal microscopy, we examined dental biofilms from healthy and caries-active subjects, revealing B-DNA, G4-, and Z-DNA structures surrounding clusters of bacteria, with some structures directly associated with the bacterial cell surface. We demonstrated that these non-canonical DNA structures were resistant to mammalian DNase I. Using a Streptococcus mutans biofilm model, we visualised fluorescently labelled eDNA during enzyme treatment and identified both an experimental nuclease and a DNase I-chloroquine combination capable of removing eDNA that was resistant to DNase I. These findings suggest that G4 and Z-DNA structures represent novel targets for improved enzyme formulations in controlling dental biofilms and potentially other biofilms containing these secondary DNA structures.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"80"},"PeriodicalIF":7.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gender differences in global antimicrobial resistance. 全球抗菌素耐药性的性别差异。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-19 DOI: 10.1038/s41522-025-00715-9

Mahkameh Salehi, Ville Laitinen, Shivang Bhanushali, Johan Bengtsson-Palme, Peter Collignon, John J Beggs, Katariina Pärnänen, Leo Lahti

{"title":"Gender differences in global antimicrobial resistance.","authors":"Mahkameh Salehi, Ville Laitinen, Shivang Bhanushali, Johan Bengtsson-Palme, Peter Collignon, John J Beggs, Katariina Pärnänen, Leo Lahti","doi":"10.1038/s41522-025-00715-9","DOIUrl":"10.1038/s41522-025-00715-9","url":null,"abstract":"Antimicrobial resistance is one of the leading causes of mortality globally. However, little is known about the distribution of antibiotic resistance genes (ARGs) in human gut metagenomes, collectively referred to as the resistome, across socio-demographic gradients. In particular, limited evidence exists on gender-based differences. We investigated how the resistomes differ between women and men in a global dataset of 14,641 publicly available human gut metagenomes encompassing countries with widely variable economic statuses. We observed a 9% higher total ARG load in women than in men in high-income countries. However, in low- and middle-income countries, the difference between genders was reversed in univariate models, but not significant after adjusting for covariates. Interestingly, the differences in ARG load between genders emerged in adulthood, suggesting resistomes differentiate between genders after childhood. Collectively, our data-driven analyses shed light on global, gendered antibiotic resistance patterns, which may help guide further research and targeted interventions.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"79"},"PeriodicalIF":7.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extensively acquired antimicrobial-resistant bacteria restructure the individual microbial community in post-antibiotic conditions. 广泛获得的抗菌素耐药细菌重组后抗生素条件下的个体微生物群落。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-14 DOI: 10.1038/s41522-025-00705-x

Jae Woo Baek, Songwon Lim, Nayeon Park, Byeongsop Song, Nikhil Kirtipal, Jens Nielsen, Adil Mardinoglu, Saeed Shoaie, Jae-Il Kim, Jang Won Son, Ara Koh, Sunjae Lee

{"title":"Extensively acquired antimicrobial-resistant bacteria restructure the individual microbial community in post-antibiotic conditions.","authors":"Jae Woo Baek, Songwon Lim, Nayeon Park, Byeongsop Song, Nikhil Kirtipal, Jens Nielsen, Adil Mardinoglu, Saeed Shoaie, Jae-Il Kim, Jang Won Son, Ara Koh, Sunjae Lee","doi":"10.1038/s41522-025-00705-x","DOIUrl":"10.1038/s41522-025-00705-x","url":null,"abstract":"In recent years, the overuse of antibiotics has led to the emergence of antimicrobial-resistant (AMR) bacteria. To evaluate the spread of AMR bacteria, the reservoir of AMR genes (resistome) has been identified in environmental samples, hospital environments, and human populations, but the functional role of AMR bacteria and their persistence within individuals has not been fully investigated. Here, we performed a strain-resolved in-depth analysis of the resistome changes by reconstructing a large number of metagenome-assembled genomes from the gut microbiome of an antibiotic-treated individual. Interestingly, we identified two bacterial populations with different resistome profiles: extensively acquired antimicrobial-resistant bacteria (EARB) and sporadically acquired antimicrobial-resistant bacteria, and found that EARB showed broader drug resistance and a significant functional role in shaping individual microbiome composition after antibiotic treatment. Our findings of AMR bacteria would provide a new avenue for controlling the spread of AMR bacteria in the human community.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"78"},"PeriodicalIF":7.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SadB, a mediator of AmrZ proteolysis and biofilm development in Pseudomonas aeruginosa. 铜绿假单胞菌AmrZ蛋白水解和生物膜发育的介质SadB。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-05-13 DOI: 10.1038/s41522-025-00710-0

Yossi Ben-David, Michael Sporny, Yigal Brochin, Bar Piscon, Shira Roth, Itzhak Zander, Michal Nisani, Sivan Shoshani, Orly Yaron, Sarit Karako-Lampert, Ilana Lebenthal-Loinger, Amos Danielli, Yarden Opatowsky, Ehud Banin

引用次数: 0