M.marinum lacking epsH shows increased biofilm formation in vitro and boosted antibiotic tolerance in zebrafish.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2025-06-14 DOI:10.1038/s41522-025-00743-5

Saara Lehmusvaara, Alina Sillanpää, Milan Wouters, Rosa Korhonen, Nelli Vahvelainen, Hanna Luukinen, Paulina Deptula, Kirsi Savijoki, Milka Hammarén, Mataleena Parikka

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Abstract

Recent discoveries have indicated that biofilm communities may play a role in natural drug tolerance of Mycobacterium tuberculosis. A transposon-based mutation library of a closely related species, Mycobacterium marinum, was used to identify clones in which the relative amount of extracellular DNA (eDNA), an important component of the extracellular matrix of biofilms, is altered. The disruption of a putative glycosyl transferase gene QDR78 11175, epsH, caused a substantial increase of the eDNA content of biofilms, and increased the growth rate and the biomass/cell in biofilm-forming conditions compared to wild-type. The increased abundance of biomass was mainly due to the elevated levels of eDNA and proteins in the extracellular matrix. The growth of the ΔepsH strain in the zebrafish was normal, but the mutant developed greater antibiotic tolerance in the adult zebrafish model. These results suggest that the extracellular matrix of biofilms increases antibiotic tolerance of mycobacteria during infection.

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缺乏epsH的marinum在斑马鱼中显示出体外生物膜形成增加和抗生素耐受性增强。

最近的发现表明，生物膜群落可能在结核分枝杆菌的天然耐药中起作用。一个基于转座子的突变文库是一种密切相关的物种，海洋分枝杆菌，用于鉴定细胞外DNA （eDNA）的相对数量发生改变的克隆，eDNA是生物膜细胞外基质的重要组成部分。与野生型相比，糖基转移酶QDR78 11175基因epsH的破坏导致生物膜eDNA含量大幅增加，生物膜形成条件下的生长速度和生物量/细胞数增加。生物量丰度的增加主要是由于细胞外基质中eDNA和蛋白质水平的升高。ΔepsH菌株在斑马鱼体内生长正常，但突变体在成年斑马鱼模型中表现出更大的抗生素耐受性。这些结果表明，在感染过程中，生物膜的细胞外基质增加了分枝杆菌的抗生素耐受性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.