微流体揭示了重力和剪切应力对荧光假单胞菌运动和生物膜生长的作用。

IF 9.2 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Daniele Marra, Moreno Rizzo, Sergio Caserta
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引用次数: 0

摘要

生物膜在密闭环境中的增殖是生物医学、工业和空间应用中的一个挑战。由于流动和重力,与流体接触的表面经历不同的体积应力,这些因素在生物膜研究中经常被忽视。本研究量化了重力和剪切应力对荧光假单胞菌SBW25运动和生物膜生长的联合影响。利用层流下的矩形截面微流体通道,我们比较了顶部和底部表面,重力将细菌拉离或将它们推向表面。结果显示细菌分布不对称,导致不同的表面细胞密度和污染水平。我们还分析了随时间的空间重组,并对细菌在水流下的运动进行了分类。研究结果表明,外部机械应力影响生物膜的运动和形态,影响基于剪切应力和重力方向的生物污染模式。该研究为不同环境下的生物膜控制策略提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microfluidics unveils role of gravity and shear stress on Pseudomonas fluorescens motility and biofilm growth.

Biofilm proliferation in confined environments is a challenge in biomedical, industrial, and space applications. Surfaces in contact with fluids experience varying bulk stresses due to flow and gravity, factors often overlooked in biofilm studies. This research quantifies the combined effect of gravity and shear stress on Pseudomonas fluorescens SBW25 motility and biofilm growth. Using a rectangular-section microfluidic channel under laminar flow, we compared top and bottom surfaces, where gravity either pulls bacteria away or pushes them toward the surface. Results revealed an asymmetric bacterial distribution, leading to varying surface cell densities and contamination levels. We also analyzed spatial reorganization over time and classified bacterial motility under flow. Findings show that external mechanical stresses influence both motility and biofilm morphology, impacting biocontamination patterns based on shear stress and gravity direction. This study provides insights into biofilm control strategies in diverse environments.

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来源期刊
npj Biofilms and Microbiomes
npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology
CiteScore
12.10
自引率
3.30%
发文量
91
审稿时长
9 weeks
期刊介绍: npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.
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