{"title":"微流体揭示了重力和剪切应力对荧光假单胞菌运动和生物膜生长的作用。","authors":"Daniele Marra, Moreno Rizzo, Sergio Caserta","doi":"10.1038/s41522-025-00744-4","DOIUrl":null,"url":null,"abstract":"<p><p>Biofilm proliferation in confined environments is a challenge in biomedical, industrial, and space applications. Surfaces in contact with fluids experience varying bulk stresses due to flow and gravity, factors often overlooked in biofilm studies. This research quantifies the combined effect of gravity and shear stress on Pseudomonas fluorescens SBW25 motility and biofilm growth. Using a rectangular-section microfluidic channel under laminar flow, we compared top and bottom surfaces, where gravity either pulls bacteria away or pushes them toward the surface. Results revealed an asymmetric bacterial distribution, leading to varying surface cell densities and contamination levels. We also analyzed spatial reorganization over time and classified bacterial motility under flow. Findings show that external mechanical stresses influence both motility and biofilm morphology, impacting biocontamination patterns based on shear stress and gravity direction. This study provides insights into biofilm control strategies in diverse environments.</p>","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"122"},"PeriodicalIF":9.2000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12216817/pdf/","citationCount":"0","resultStr":"{\"title\":\"Microfluidics unveils role of gravity and shear stress on Pseudomonas fluorescens motility and biofilm growth.\",\"authors\":\"Daniele Marra, Moreno Rizzo, Sergio Caserta\",\"doi\":\"10.1038/s41522-025-00744-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Biofilm proliferation in confined environments is a challenge in biomedical, industrial, and space applications. Surfaces in contact with fluids experience varying bulk stresses due to flow and gravity, factors often overlooked in biofilm studies. This research quantifies the combined effect of gravity and shear stress on Pseudomonas fluorescens SBW25 motility and biofilm growth. Using a rectangular-section microfluidic channel under laminar flow, we compared top and bottom surfaces, where gravity either pulls bacteria away or pushes them toward the surface. Results revealed an asymmetric bacterial distribution, leading to varying surface cell densities and contamination levels. We also analyzed spatial reorganization over time and classified bacterial motility under flow. Findings show that external mechanical stresses influence both motility and biofilm morphology, impacting biocontamination patterns based on shear stress and gravity direction. This study provides insights into biofilm control strategies in diverse environments.</p>\",\"PeriodicalId\":19370,\"journal\":{\"name\":\"npj Biofilms and Microbiomes\",\"volume\":\"11 1\",\"pages\":\"122\"},\"PeriodicalIF\":9.2000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12216817/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"npj Biofilms and Microbiomes\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1038/s41522-025-00744-4\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"npj Biofilms and Microbiomes","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41522-025-00744-4","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Microfluidics unveils role of gravity and shear stress on Pseudomonas fluorescens motility and biofilm growth.
Biofilm proliferation in confined environments is a challenge in biomedical, industrial, and space applications. Surfaces in contact with fluids experience varying bulk stresses due to flow and gravity, factors often overlooked in biofilm studies. This research quantifies the combined effect of gravity and shear stress on Pseudomonas fluorescens SBW25 motility and biofilm growth. Using a rectangular-section microfluidic channel under laminar flow, we compared top and bottom surfaces, where gravity either pulls bacteria away or pushes them toward the surface. Results revealed an asymmetric bacterial distribution, leading to varying surface cell densities and contamination levels. We also analyzed spatial reorganization over time and classified bacterial motility under flow. Findings show that external mechanical stresses influence both motility and biofilm morphology, impacting biocontamination patterns based on shear stress and gravity direction. This study provides insights into biofilm control strategies in diverse environments.
期刊介绍:
npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.