Fusobacterium lineage profiling facilitates the clarification of the associations between non-nucleatum Fusobacterium and colorectal cancer.

IF 9.2 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2025-10-09 DOI:10.1038/s41522-025-00826-3

Yuli Wu, Guo Ji, Dongyan Han, Youhua Zhang, Xingchen Zhu, Hao Li, Man Li, Yaohui Gao, Ruting Xie, Min Xu, Ling Lu, Zixin Deng, Qing Wei, Huanlong Qin, Dexi Bi

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引用次数: 0

Abstract

Non-nucleatum Fusobacterium may play a nonnegligible role in colorectal cancer (CRC) and certain Fusobacterium lineages (namely, L1 and L5) have shown specific associations with CRC. We aim to clarify the complex connections between Fusobacterium and CRC. We found that the widely adopted quantitative PCR (qPCR) method could overestimate F. nucleatum abundance and, in fact, reflect L1 levels in clinical samples. A lineage-specific qPCR assay targeting L1/L5 was developed and validated using mock and clinical samples. Its application in independent cohorts confirmed that L1 was overabundant in CRC, whereas L5 correlated with lymphovascular invasion. Importantly, faecal L1 abundance was more predictive of CRC than F. nucleatum, supported also by cross-population metagenomic data. CRC-associated virulence and colonisation genes were found in various L1 species other than F. nucleatum. Our results highlight the clinical importance of L1/L5 in CRC with high-diversity Fusobacterium contexts and suggest that non-nucleatum Fusobacterium may also contribute to CRC.

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梭杆菌谱系分析有助于澄清非核梭杆菌与结直肠癌之间的关系。

非核梭杆菌可能在结直肠癌（CRC）中发挥不可忽视的作用，某些梭杆菌谱系（即L1和L5）已显示出与CRC的特定关联。我们旨在阐明梭杆菌与结直肠癌之间的复杂联系。我们发现广泛采用的定量PCR （qPCR）方法可能高估了具核梭菌的丰度，实际上反映了临床样品中的L1水平。开发了针对L1/L5的谱系特异性qPCR检测方法，并使用模拟和临床样本进行了验证。其在独立队列中的应用证实了L1在结直肠癌中过富集，而L5与淋巴血管侵袭相关。重要的是，粪便L1丰度比具核梭菌更能预测结直肠癌，这也得到了跨种群宏基因组数据的支持。除核仁梭菌外，在各种L1种中均发现了与crc相关的毒力和定植基因。我们的研究结果强调了L1/L5在具有高多样性梭杆菌背景的结直肠癌中的临床重要性，并表明非核梭杆菌也可能导致结直肠癌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.