npj Biofilms and Microbiomes最新文献_第8页

Oat bran fiber protects against radiation-induced disruption of gut barrier dynamics and mucosal damage. 燕麦麸皮纤维可以防止辐射引起的肠道屏障动力学破坏和粘膜损伤。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-04 DOI: 10.1038/s41522-025-00759-x

Piyush Patel, Chunsheng Jin, Intawat Nookaew, Michael Robeson, Dilip K Malipatlolla, Sravani Devarakonda, Ana Rascón, Margareta Nyman, Niclas G Karlsson, Agnes E Wold, Fei Sjöberg, Cecilia Bull

{"title":"Oat bran fiber protects against radiation-induced disruption of gut barrier dynamics and mucosal damage.","authors":"Piyush Patel, Chunsheng Jin, Intawat Nookaew, Michael Robeson, Dilip K Malipatlolla, Sravani Devarakonda, Ana Rascón, Margareta Nyman, Niclas G Karlsson, Agnes E Wold, Fei Sjöberg, Cecilia Bull","doi":"10.1038/s41522-025-00759-x","DOIUrl":"10.1038/s41522-025-00759-x","url":null,"abstract":"Dietary fibers are recognized for their health benefits, yet cancer patients undergoing pelvic radiotherapy are often advised to reduce fiber intake. This may negatively impact their bowel health. To evaluate the effects of dietary fibers on bowel health post-irradiation, male C57BL/6 mice were fed diets containing either 0 or 15% fiber with varying proportions of readily fermentable bioprocessed oat bran fiber and/or poorly fermentable microcrystalline cellulose, and either irradiated or sham-irradiated. Irradiation triggered mucus degradation and depletion of short-chain fatty acids, and a fiber-free diet exacerbated radiation-induced mucosal damage. In contrast, mice fed oat bran fiber exhibited less mucosal damage, fewer dysbiotic and mucus-degrading bacteria, higher production of short-chain fatty acids, and improved bactericidal activity. These benefits were dose-dependent, with 15% oat bran fiber providing greater protection. Our findings suggest that fiber deprivation exacerbates radiation-induced intestinal damage, while supplementation with 15% highly fermentable oat bran fiber supports mucosal integrity and protects against radiation-induced injury.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"128"},"PeriodicalIF":7.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144565055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus secretes nucleases to degrade dsRNA, thereby reducing host's susceptibility to RNAi. 芽孢杆菌分泌核酸酶降解dsRNA，从而降低宿主对RNAi的易感性。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-03 DOI: 10.1038/s41522-025-00757-z

Xinyi Han, Haichao Li, Saibo Xu, Xuexia Miao, Ruobing Guan

{"title":"Bacillus secretes nucleases to degrade dsRNA, thereby reducing host's susceptibility to RNAi.","authors":"Xinyi Han, Haichao Li, Saibo Xu, Xuexia Miao, Ruobing Guan","doi":"10.1038/s41522-025-00757-z","DOIUrl":"10.1038/s41522-025-00757-z","url":null,"abstract":"RNAi technology, which can induce mortality by disrupting the transcription of essential growth and development-related genes in insects, has emerged as a groundbreaking pest control method. However, insects have developed defense mechanisms to counteract the efficiency of RNAi. The specific role of symbiotic microorganisms in this process remains poorly understood and requires further exploration. This study examines the reduced RNAi efficiency in Lepidopteran pest Helicoverpa armigera. Through screening, six Bacillus strains exhibiting dsRNA-degrading activity were identified through in vitro assays. Further investigation into one representative strain Ba 6 revealed that it significantly decreased RNAi efficiency by secreting ribonuclease into the insect gut fluid, directly degrading dsRNA, thus reducing its accumulation and blocking RNAi effects. These findings clarify the mechanism by which symbiotic bacteria influence the host's RNAi efficiency and provides a valuable reference for the development and large-scale implementation of RNA biopesticides targeting H. armigera and other lepidopteran pests.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"127"},"PeriodicalIF":7.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: Bacterial biofilm-derived H-NS protein acts as a defense against Neutrophil Extracellular Traps (NETs). 作者更正：细菌生物膜衍生的H-NS蛋白作为对中性粒细胞胞外陷阱（NETs）的防御。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-02 DOI: 10.1038/s41522-025-00761-3

A L Hendricks, K R More, A Devaraj, J R Buzzo, F H Robledo-Avila, S Partida-Sanchez, L O Bakaletz, S D Goodman

引用次数: 0

Mother-to-infant vertical transmission in early life: a systematic review and proportional meta-analysis of Bifidobacterium strain transmissibility. 早期母婴垂直传播：双歧杆菌菌株传播率的系统回顾和比例荟萃分析。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-01 DOI: 10.1038/s41522-025-00720-y

Eduard Flores Ventura, María Esteban-Torres, Miguel Gueimonde, Douwe van Sinderen, Omry Koren, Lindsay J Hall, Nicola Segata, Mireia Valles-Colomer, Maria Carmen Collado

{"title":"Mother-to-infant vertical transmission in early life: a systematic review and proportional meta-analysis of Bifidobacterium strain transmissibility.","authors":"Eduard Flores Ventura, María Esteban-Torres, Miguel Gueimonde, Douwe van Sinderen, Omry Koren, Lindsay J Hall, Nicola Segata, Mireia Valles-Colomer, Maria Carmen Collado","doi":"10.1038/s41522-025-00720-y","DOIUrl":"10.1038/s41522-025-00720-y","url":null,"abstract":"Early-life colonization is a critical developmental process influencing infant biological programming, with bifidobacteria playing a key role. This systematic review examines the transmissibility of Bifidobacterium strains from mothers to infants. Adhering to Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines, 31 articles from 2009 to 2024 were selected from 2825 screened titles and abstracts. Using a narrative synthesis and meta-analysis, the review focuses on studies employing strain-level metagenomic approaches (Protocol registry CRD: CRD42023490507). Ten studies using shotgun metagenomic sequencing identified specific strains of B. adolescentis, B. angulatum, B. bifidum, B. breve, B. pseudocatenulatum, B. catenulatum, and B. longum shared between mothers and infants. A meta-analysis of 810 mother-infant pairs revealed an overall species transmissibility estimate of 30% (95% CI: 0.17; 0.44), with B. longum strains persisting in infants' guts for up to 6 months. Strain transmissibility was higher in vaginally delivered infants compared to those delivered by caesarean section. This review highlights the high transmission rates of maternal Bifidobacterium strains in early-life gut seeding, particularly B. bifidum and B. longum. Despite ongoing research, uncertainties remain regarding the precise characteristics, transmission routes, and mechanisms of transmitted strains. Comprehensive approaches, including metagenomic sequencing and longitudinal studies, are needed to understand the role of vertical transmission in infant gut microbiome engraftment and its functional implications.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"121"},"PeriodicalIF":7.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlling metabolic stability of food microbiome for stable indigenous liquor fermentation. 控制食物微生物群代谢稳定性对稳定本土白酒发酵的影响。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-01 DOI: 10.1038/s41522-025-00729-3

Yuwei Tan, Yang Zhu, René H Wijffels, Hongxia Zhang, William T Scott, Yan Xu, Vitor Martins Dos Santos

{"title":"Controlling metabolic stability of food microbiome for stable indigenous liquor fermentation.","authors":"Yuwei Tan, Yang Zhu, René H Wijffels, Hongxia Zhang, William T Scott, Yan Xu, Vitor Martins Dos Santos","doi":"10.1038/s41522-025-00729-3","DOIUrl":"10.1038/s41522-025-00729-3","url":null,"abstract":"Steering microbial metabolic stability in fermentation is a recurrent goal in microbial food production. Indigenous liquor fermentation typically relies on complex microbiome metabolism, making it difficult to steer fermentation towards consistent high-quality products. Here, we conducted a three-step experiment to identify instability factors and explore ways to steer fermentation stability in lab-scale settings. We found that the metabolic stability of the microbiome fluctuates due to fermentation parameters, dynamic benefit allocation between yeasts and Lactobacilli, and metabolic network redundancy. In addition to parameters control, short-term metabolic stability requires stable microbial benefit allocation, whereas long-term stability requires proper functional redundancy. Rationally setting initial parameters and the microbial inoculation ratio is a practical way to optimize metabolic stability for stable indigenous liquor fermentation. Our study provides new insights into microbiome metabolism control and shows the feasibility of enhancing fermentation stability through appropriate initial conditions, enabling more controlled and efficient microbial food production systems.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"124"},"PeriodicalIF":7.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12214495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaginal pharmacomicrobiomics modulates risk of persistent and recurrent bacterial vaginosis. 阴道药物微生物组学调节持续性和复发性细菌性阴道病的风险。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-01 DOI: 10.1038/s41522-025-00748-0

Emmanuel Amabebe, Madhuri Tatiparthy, Ananth K Kammala, Lauren S Richardson, Brandie D Taylor, Surendra Sharma, Ramkumar Menon

{"title":"Vaginal pharmacomicrobiomics modulates risk of persistent and recurrent bacterial vaginosis.","authors":"Emmanuel Amabebe, Madhuri Tatiparthy, Ananth K Kammala, Lauren S Richardson, Brandie D Taylor, Surendra Sharma, Ramkumar Menon","doi":"10.1038/s41522-025-00748-0","DOIUrl":"10.1038/s41522-025-00748-0","url":null,"abstract":"Bacterial vaginosis (BV) is the most commonly diagnosed vaginal infection in women of reproductive age, with most patients unaware that they have BV due to its asymptomatic nature. BV is a dysbiotic condition defined by a deviation from the healthy Lactobacillus dominance to a polymicrobial anaerobic bacterial community that increases the risk of sexually transmitted infections and adverse reproductive outcomes, including spontaneous preterm birth. The increasing number of infectious agents in BV, biofilm persistence and antibiotic resistance in the vaginal canal hinder effective treatments with antibiotics leading to consistent recurrence of BV in many women (30-70%). Like in the gut, these vaginal drug-microbiome interactions termed pharmacomicrobiomics could alter drug disposition, mechanism of action, and toxicity that reduce the efficacy of antibiotics and increase the risk of persistent and recurrent BV and its sequelae. For instance, both vaginal epithelial and bacterial cells co-exist and possess enzymes that metabolize antibiotics, and transporter proteins that expel drugs and toxins, rendering them ineffective. Despite significant progress on pharmacomicrobiomics in the gut, little is known about this phenomenon in the vaginal microenvironment, which harbors a consequential microbiota and a major source of infection and antibiotic resistance. Therefore, to improve therapeutic outcomes and reduce the rate of persistent/recurrent BV and infection-associated preterm birth, we present an overview of the evidence pertaining to the effect of vaginal microbiome-drug interactions and efficacy of antibiotics against recurrent BV. We also highlight plausible mechanistic underpinnings of these interactions and implications for treatment modalities to combat infection-associated preterm birth.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"115"},"PeriodicalIF":7.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Berberine chloride hydrate impairs Streptococcus mutans biofilm formation via inhibiting sortase A activity. 氯化小檗碱通过抑制分选酶A活性损害变形链球菌生物膜的形成。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-01 DOI: 10.1038/s41522-025-00756-0

Yang Zhou, Fang Huang, Huancai Lin

{"title":"Berberine chloride hydrate impairs Streptococcus mutans biofilm formation via inhibiting sortase A activity.","authors":"Yang Zhou, Fang Huang, Huancai Lin","doi":"10.1038/s41522-025-00756-0","DOIUrl":"10.1038/s41522-025-00756-0","url":null,"abstract":"Dental caries is a biofilm-associated chronic progressive disease, results from the dissolution of mineralized tooth tissue by acidic generated from bacterial sugar fermentation. S. mutans, a prominent pathogen of dental caries, is acknowledged for its role in cariogenic biofilm formation, utilizing Sortase A (SrtA) to catalyse surface proteins, thus promoting biofilm formation. In our previous studies, the inhibitory effect of the berberine chloride hydrate (BH) on S. mutans biofilms was confirmed. Here, we further investigate the influence of BH on S. mutans biofilm-induced bovine enamel caries model and explore the effect of BH on S. mutans SrtA activity. We found that BH inhibited S. mutans biofilm formation in bovine enamel model, leading to a reduction in demineralization. Furthermore, we identified and characterized SrtA, which might catalyze SpaP of S. mutans to form fibrillar amyloid aggregates. Our findings showed that BH inhibited SrtA activity by binding to essential amino acid residues LEU-111, MET-123, and ARG-213. BH inhibited amyloid fibers formation by downregulating the expression of srtA gene, thus disrupting S. mutans biofilm formation. Taken together, our study provides new insight into the mechanism of antibiofilm activity of BH and reveals great potential for anticaries clinical applications.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"120"},"PeriodicalIF":7.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of genotypic and phenotypic diversity in multispecies biofilms. 多物种生物膜基因型和表型多样性的进化。

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-01 DOI: 10.1038/s41522-025-00755-1

Cristina I Amador, Sofia Zoe Moscovitz, Lorrie Maccario, Jakob Herschend, Isabel-Sophie Kramer, Hannah Jeckel, Vaughn S Cooper, Knut Drescher, Thomas R Neu, Mette Burmølle, Henriette L Røder

{"title":"Evolution of genotypic and phenotypic diversity in multispecies biofilms.","authors":"Cristina I Amador, Sofia Zoe Moscovitz, Lorrie Maccario, Jakob Herschend, Isabel-Sophie Kramer, Hannah Jeckel, Vaughn S Cooper, Knut Drescher, Thomas R Neu, Mette Burmølle, Henriette L Røder","doi":"10.1038/s41522-025-00755-1","DOIUrl":"10.1038/s41522-025-00755-1","url":null,"abstract":"Bacterial fitness and adaptability in microbial communities are influenced by interspecies interactions and spatial organization. This study investigated how these factors shape the evolutionary dynamics of Bacillus thuringiensis. A distinct phenotypic variant of B. thuringiensis emerged consistently under both planktonic and biofilm conditions, as well as in monospecies and mixed-species settings, but was strongly selected in biofilms and during coexistence with Pseudomonas defluvii and/or Pseudomonas brenneri. Compared to its ancestor, the variant exhibited shorter generation times, reduced sporulation, auto-aggregation, and lower biomass in mixed-species biofilms. Mutations in the spo0A regulator, which controls sporulation and biofilm matrix production, were identified in all variants. Proteomics revealed a reduction in TasA, a key matrix protein, in the variant but increased levels in co-culture with P. brenneri. These findings highlight how interspecies interactions drive B. thuringiensis diversification, promoting traits like reduced matrix production and species coexistence, with implications for microbial consortia applications in agriculture and biopesticides.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"118"},"PeriodicalIF":7.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12216329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-microbiome relationship in depression: can human induced pluripotent stem cells play a role in unravelling mechanisms? 抑郁症的宿主-微生物组关系：人类诱导的多能干细胞能否在揭示机制中发挥作用？

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-01 DOI: 10.1038/s41522-025-00749-z

Cristina Rosell-Cardona, John F Cryan, Gerard Clarke, Sarah Kittel-Schneider

引用次数: 0

IF 7.8 1区生物学

npj Biofilms and Microbiomes Pub Date : 2025-07-01 DOI: 10.1038/s41522-025-00724-8

Kevin H Mwangi, Yue Qu, Peilun Hu, Toshitatsu Nagayasu, Jia-Feng Liu, Xiumei Wang

{"title":"Microanatomy related biocidal activity at cellular resolution and bone reconstruction potential of PEG EGaIn nanocapsules.","authors":"Kevin H Mwangi, Yue Qu, Peilun Hu, Toshitatsu Nagayasu, Jia-Feng Liu, Xiumei Wang","doi":"10.1038/s41522-025-00724-8","DOIUrl":"10.1038/s41522-025-00724-8","url":null,"abstract":"Critical bone defects, exacerbated by infections, pose significant challenges to bone healing and homeostasis, necessitating the development of dual-functional biomimetics that combine anti-infective and reparative capabilities. The EGaIn holds promise across various disciplines, though its interactions with microbial cells require further elucidation. This investigation evaluates the antimicrobial efficacy of PEG-EGaIn nanocapsules against a spectrum of bacterial, employing electron microscopy. Constructs containing 1.5% PEG-EGaIn hinder biofilm-producing bacteria, while 3% concentrations amplify the biocidal effect. Furthermore, the nanocapsules promoted osteogenic differentiation rBMSCs, evidenced by enhanced mineralization and upregulation of key osteogenic genes. In addressing large bone defects, PEG-EGaIn-Col-Ap-lamellar and ethanolic-mediated Col-Ap-lamellar constructs serve as potential solutions for bone resorption mitigation and osteo-angiogenesis. Bone-remodeling were validated through μ-CT and histomorphometry confirming no evidence of chronic inflammation or fibrosis. In this study, PEG-EGaIn nanocapsules emerge as potent biocide and bone repair, underscoring their potential in combating antibiotic resistance and enhancing bone healing.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"11 1","pages":"123"},"PeriodicalIF":7.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12216455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144541644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0