npj Biofilms and Microbiomes最新文献_第7页

The human lung microbiome progressively diminishes in the distal alveolar regions. 人肺微生物群在远端肺泡区逐渐减少。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-15 DOI: 10.1038/s41522-026-01047-y

Pauline Potratz, Antje Häder, Laura Kursawe, Judith Kikhney, Nikolaus Gaßler, Thurid Lauf, Lukáš Radosa, Tim Sandhaus, Patrick von Samson, Xiuqiang Chen, Lu Wang, Axel A Brakhage, Annette Moter, Gianni Panagiotou, Torsten Doenst, Stefanie Deinhardt-Emmer, Bettina Löffler

{"title":"The human lung microbiome progressively diminishes in the distal alveolar regions.","authors":"Pauline Potratz, Antje Häder, Laura Kursawe, Judith Kikhney, Nikolaus Gaßler, Thurid Lauf, Lukáš Radosa, Tim Sandhaus, Patrick von Samson, Xiuqiang Chen, Lu Wang, Axel A Brakhage, Annette Moter, Gianni Panagiotou, Torsten Doenst, Stefanie Deinhardt-Emmer, Bettina Löffler","doi":"10.1038/s41522-026-01047-y","DOIUrl":"10.1038/s41522-026-01047-y","url":null,"abstract":"Extensive investigations with sequencing methods demonstrate a large and diverse microbiome even in profound areas of the lung. However, there is still substantial lack of cultivation-based evidence and of the viability of the resident microorganisms. We collected human tissue specimens obtained from various regions during lung transplantations and from operations in distal alveolar areas. We characterized the samples by histology and applied sequencing, culture and imaging methods. Sequencing data detected the following trends in the composition of the microbiome: (i) From proximal to distal samples we observed a bacterial shift from Staphylococci, Streptococci and Corynebacteria towards preferentially anaerobically growing bacteria; (ii) we found large variations between individual patients regarding the detected bacterial genera. Culture and imaging methods revealed almost no viable microorganism in the distal alveolar regions in these patients. Bacterial signals detected by sequencing in the lung are likely due to very low numbers of bacteria and/or their remnants.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13287667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intermittent fasting exacerbates microplastic-induced gut inflammation via Rikenella-mediated regulation of purine metabolism and Th17/Treg balance. 间歇性禁食通过里氏菌介导的嘌呤代谢和Th17/Treg平衡调节加剧微塑料诱导的肠道炎症。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-15 DOI: 10.1038/s41522-026-01016-5

Rui Fu, Yixian Cheng, Junjie Chen, Weiwei Sheng, Jinghua Gu, Jiahao Wang, Zheng Zhang, Huake Cao, Jiawei Zhang, Bo Chen, Guodong Cao

{"title":"Intermittent fasting exacerbates microplastic-induced gut inflammation via Rikenella-mediated regulation of purine metabolism and Th17/Treg balance.","authors":"Rui Fu, Yixian Cheng, Junjie Chen, Weiwei Sheng, Jinghua Gu, Jiahao Wang, Zheng Zhang, Huake Cao, Jiawei Zhang, Bo Chen, Guodong Cao","doi":"10.1038/s41522-026-01016-5","DOIUrl":"10.1038/s41522-026-01016-5","url":null,"abstract":"The pervasive accumulation of microplastics (MPs) in ecosystems and human bodies has prompted growing concern regarding their biological effects. However, whether intermittent fasting (IF) modulates MP-induced gut injury remains unclear. We established a murine model of chronic oral exposure to 1 μm polystyrene MPs combined with a daily 8-h feeding window. Multi-omics analyses, including 16S rRNA sequencing, untargeted metabolomics, and immune profiling, were performed. In a murine model of chronic oral exposure to 1 μm polystyrene microplastics, IF aggravated colonic inflammation and oxidative stress. 16S rRNA sequencing showed that IF further altered the microplastic-associated gut microbiota, notably reducing Rikenella abundance. Untargeted metabolomics revealed disrupted purine metabolism, including decreased hypoxanthine levels, which positively correlated with Rikenella. Combined MP and IF exposure was associated with an increased Th17/Treg ratio. Supplementation with Rikenella or hypoxanthine partially attenuated inflammatory changes. These findings unveil a previously unappreciated risk of IF under conditions of environmental microplastic exposure, and highlight the necessity of integrating environmental toxicants into evaluations of dietary interventions.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13677689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148258988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phyllosphere microbiome responses to spray-induced gene silencing targeting Phytophthora infestans in potato. 马铃薯层球微生物组对喷雾诱导的马铃薯疫霉基因沉默的响应

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-15 DOI: 10.1038/s41522-026-01040-5

Samrat Ghosh, Poorva Sundararajan, Bekele Gelena Kelbessa, Farideh Ghadamgahi, Stephen C Whisson, Mukesh Dubey, Aakash Chawade, Ramesh R Vetukuri

{"title":"Phyllosphere microbiome responses to spray-induced gene silencing targeting Phytophthora infestans in potato.","authors":"Samrat Ghosh, Poorva Sundararajan, Bekele Gelena Kelbessa, Farideh Ghadamgahi, Stephen C Whisson, Mukesh Dubey, Aakash Chawade, Ramesh R Vetukuri","doi":"10.1038/s41522-026-01040-5","DOIUrl":"10.1038/s41522-026-01040-5","url":null,"abstract":"Recent years have seen a growing interest in the use of RNA interference to control filamentous pathogens, creating a new niche in plant disease management. Strategies like spray-induced gene silencing (SIGS) offer effective and environmentally friendly alternatives to chemical disease control but remain underexplored. Given the profound influence of microbiomes on plant health and crop productivity, knowledge of how spraying double-stranded RNAs (dsRNAs) can impact plant microbial communities is needed to facilitate the transition of SIGS from the laboratory to practical large-scale use. We have therefore investigated changes in the bacterial and fungal communities of the phyllosphere in the economically important potato plant after spraying with dsRNA targeting the phytopathogen Phytophthora infestans. Spraying with dsRNA alone had little effect on the relative abundance of the dominant species found in the native potato phyllosphere. However, there were small time-dependent changes in the composition of the bacterial communities, and much larger changes in bacterial community metrics were observed after P. infestans inoculation. We also observed maintenance of potentially beneficial bacterial genera in dsRNA-treated plants in addition to composition changes linked to the plant's natural defense response upon P. infestans infection. Together, these observations support the view that dsRNA spraying enables safe and targeted pathogen control in potato.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"12 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Goat gut microbiome as a reservoir for microorganism-encoded short peptides: regulation by host development age and nematode challenge. 山羊肠道微生物组作为微生物编码短肽的储存库：宿主发育年龄和线虫挑战的调节。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-13 DOI: 10.1038/s41522-026-01051-2

Qiongxian Yan, Wenxun Chen, Jinhe Kang, Xiaoling Zhou, Zhixiong He, Zhiliang Tan

{"title":"Goat gut microbiome as a reservoir for microorganism-encoded short peptides: regulation by host development age and nematode challenge.","authors":"Qiongxian Yan, Wenxun Chen, Jinhe Kang, Xiaoling Zhou, Zhixiong He, Zhiliang Tan","doi":"10.1038/s41522-026-01051-2","DOIUrl":"10.1038/s41522-026-01051-2","url":null,"abstract":"Short peptides are a promising class of antimicrobial agents with efficacy against multidrug-resistant pathogens. While host-derived defense peptides from animals and plants have been widely characterized, the landscape of microbial-encoded short peptides, particularly those with antimicrobial activity that can mitigate antibiotic resistance, remains underexplored. In this study, we identified 1147 novel microorganism-encoded short peptides (MSPs) from the goat gut microbiome, most of which lack homology to existing database entries. The microbial communities producing these MSPs were altered by the developmental stage of the goat, whereas the overall composition and diversity of these populations remained unchanged during nematode infection, despite significant correlations between certain MSPs and type 2 immunity. Notably, two MSPs demonstrated potent antimicrobial activity with lower cytotoxicity, exhibiting remarkable efficacy against methicillin-resistant Staphylococcus aureus and Streptococcus agalactiae. Collectively, our findings establish the goat gastrointestinal tract microbiome as a distinctive and promising reservoir for the discovery of antimicrobial and immunoregulatory peptides.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13619559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding phage communication: molecular networks, evolutionary dynamics, and therapeutic applications. 解码噬菌体通讯：分子网络、进化动力学和治疗应用。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-12 DOI: 10.1038/s41522-026-01050-3

Sahar Abed, Mohammad Sholeh, Sara Sadeghi, Ameneh Khatami, Peter Speck, Morvarid Shafiei

引用次数: 0

High early-life gut Bacteroides links to microbiome stability, resilience, and risk for childhood infections. 生命早期高水平的肠道拟杆菌与微生物群的稳定性、复原力和儿童感染风险有关。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-11 DOI: 10.1038/s41522-026-01022-7

Matthias Hauptmann, Cornelia Gottschick, Uthayakumar Muthukumarasamy, Bianca Klee, Till Strowig, Rafael Mikolajczyk, Ulrich Emil Schaible

{"title":"High early-life gut Bacteroides links to microbiome stability, resilience, and risk for childhood infections.","authors":"Matthias Hauptmann, Cornelia Gottschick, Uthayakumar Muthukumarasamy, Bianca Klee, Till Strowig, Rafael Mikolajczyk, Ulrich Emil Schaible","doi":"10.1038/s41522-026-01022-7","DOIUrl":"10.1038/s41522-026-01022-7","url":null,"abstract":"Early childhood events, up to the age of two, are critical for the development of the microbiome and balanced immunity later in life. We investigated whether susceptibility to infections and microbiome resilience after antibiotic treatment are associated with key taxa in the early childhood microbiota. Therefore, we performed longitudinal microbiota analysis from stool samples of children within the German LoewenKIDS intensified subcohort. According to the exposure to antibiotic treatment, sample groups were defined as never-treated controls, 45-225 days pre-treatment (pre45-225), 0-30 days pre-treatment (pre0-30), 0-30 days post-treatment (post0-30), or >90 days post-treatment and age >540 days (post>90). 1176 stool samples of 162 children were included in our analysis, of which 49 children received antibiotics. Using generalized linear mixed models adjusted for age, we show that high abundance of Bacteroides was associated with receiving antibiotic treatment 45-225 days later, while low Bacteroides abundance before treatment was associated with low alpha diversity and increased beta diversity post treatment. Our data suggest a key role of the genus Bacteroides for the susceptibility to infections requiring antibiotic treatment and for microbiome stability and resilience in early childhood.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":"12 1","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13254207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermented feed-rumen microbiota axis mediates goat kid growth via microbial amino acid synthesis and ruminal epithelial energy metabolism. 发酵饲料-瘤胃微生物群轴通过微生物氨基酸合成和瘤胃上皮能量代谢介导山羊幼山羊生长。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-11 DOI: 10.1038/s41522-026-01046-z

Fanlin Kong, Fenghui Liu, Yong Qian, Ziyu Wang, Wenfeng Zhang, Yan Wang, Jianli Zhang, Yinxia Li, Jun Zhang, Shuo Wang, Dongwen Dai, Zhiping Lin, Huili Wang, Chunhua Meng

{"title":"Fermented feed-rumen microbiota axis mediates goat kid growth via microbial amino acid synthesis and ruminal epithelial energy metabolism.","authors":"Fanlin Kong, Fenghui Liu, Yong Qian, Ziyu Wang, Wenfeng Zhang, Yan Wang, Jianli Zhang, Yinxia Li, Jun Zhang, Shuo Wang, Dongwen Dai, Zhiping Lin, Huili Wang, Chunhua Meng","doi":"10.1038/s41522-026-01046-z","DOIUrl":"10.1038/s41522-026-01046-z","url":null,"abstract":"Pretreating feed via fermentation to enhance its nutritional value is effective for young ruminants. This study optimized fermented feed using a microbial consortium of Saccharomyces cerevisiae, Bacillus licheniformis, and Enterococcus faecalis, and explored its mechanisms on growth performance and ruminal function in goat kids. The Bacillus licheniformis is the most critical determinant of the nutritional value of FF. Optimized FF had higher levels of crude protein, calcium, phosphorus, and limiting amino acids (AA). The FF contained viable microbiota with lower diversity but greater evenness. The microbiota was enriched in AA and carbohydrate metabolism by enhancing the abundance of Leuconostoc lactis and Lactococcus lactis. A 40-day feeding trial was then conducted with 20-day-old goat kids. Compared to conventional feed, FF increased feed digestibility, body weight and carcass weight. Mechanistically, FF enhanced the interactions among rumen microbes and increased the gene abundance related to carbohydrate and AA metabolism. The FF then upregulated expression of mitochondrial respiratory chain-related genes in the rumen epithelium, thereby improving epithelial energy metabolism. Overall, this study shows that fermented feed optimized by microbial consortium improves goat kid growth and carcass quality through the fermented feed-rumen microbiota axis, offering a sustainable approach for ruminant production.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148227128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyruvate carboxylase is critical for biofilm formation in Mycobacterium tuberculosis. 丙酮酸羧化酶是结核分枝杆菌生物膜形成的关键。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-11 DOI: 10.1038/s41522-026-01044-1

Shweta Singh, Sapna Sharma, Ashwani Kumar

{"title":"Pyruvate carboxylase is critical for biofilm formation in Mycobacterium tuberculosis.","authors":"Shweta Singh, Sapna Sharma, Ashwani Kumar","doi":"10.1038/s41522-026-01044-1","DOIUrl":"10.1038/s41522-026-01044-1","url":null,"abstract":"Mycobacterium tuberculosis (Mtb) forms biofilms. Biofilm formation is critical in Mtb's virulence. The metabolic pathways governing mycobacterial biofilm formation remain largely unexplored. This study evaluated how different carbon sources influence Mtb biofilm formation. Fermentable substrates-glucose, glycerol, and pyruvate-significantly promoted thick, mature biofilm formation compared to non-fermentable alternatives. In this study, we analyzed the role of pyruvate carboxylase (Pca), an anaplerotic enzyme that converts pyruvate to oxaloacetate, in biofilm formation, as it regulates carbon metabolic flux into the tricarboxylic acid (TCA) cycle and gluconeogenesis. To this end, a transposon mutant of the pca gene was investigated for its ability to form biofilms. We observed that the pca transposon mutant is deficient in pellicle, submerged, and macrocolony biofilm formation. Confocal microscopy indicated that the pca mutant does not accumulate extracellular cellulose in the biofilms and has lower biomass. These defects could be rescued upon complementing the mutant with an episomal copy of the pca gene or adding glucose or pyruvate to the medium. These observations suggest that biofilm formation requires a regulated flow of carbon flux into gluconeogenesis and the TCA cycle to ensure the supply of precursors for EPS biosynthesis and sustained energy production to fuel EPS synthesis.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13612485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148227131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parental gut microbiota dysbiosis is associated with cross-generationally shared DNA methylation patterns and autism-like behaviors in offspring. 父母肠道菌群失调与后代跨代共享的DNA甲基化模式和自闭症样行为有关。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-11 DOI: 10.1038/s41522-026-01041-4

Bin Chen, Qinghua Wang, Xinxin Xue, Xin Jiang, Meilu Zhang, Changying Zhao, Chuandi Jin, Kaile Yue, Guoping Zhao, Yanan Liu, Ning Li, Lei Zhang

{"title":"Parental gut microbiota dysbiosis is associated with cross-generationally shared DNA methylation patterns and autism-like behaviors in offspring.","authors":"Bin Chen, Qinghua Wang, Xinxin Xue, Xin Jiang, Meilu Zhang, Changying Zhao, Chuandi Jin, Kaile Yue, Guoping Zhao, Yanan Liu, Ning Li, Lei Zhang","doi":"10.1038/s41522-026-01041-4","DOIUrl":"10.1038/s41522-026-01041-4","url":null,"abstract":"Autism spectrum disorder (ASD) is a complex neurodevelopmental condition arising from interactions between genetic and environmental factors. The interplay between the microbiota-gut-brain axis and epigenetics has been implicated in ASD, but the extent of their impact remains unclear. We hypothesized that parental gut microbiota dysbiosis shapes cross-generationally shared DNA methylation patterns and ASD-like phenotypes in offspring. Antibiotic-treated adult mice were colonized with fecal microbiota from children with ASD or typically developing (TD) controls, then bred. Colonization with ASD-associated microbiota did not alter parental behavior but induced ASD-like behavioral changes in offspring, accompanied by colonic inflammation and neuronal loss in the hippocampus and striatum. Targeted bisulfite sequencing revealed that ASD-associated microbiota reshaped methylation at ASD-related CpG sites in parental brain, blood, and colon, with a subset of these alterations also observed in offspring tissue. Integrative analyses revealed sex-specific networks linking gut microbes, DNA methylation, gene expression, metabolites, and behavior. These findings support a model in which parental gut microbiota dysbiosis is associated with cross-generationally shared DNA methylation changes linked to ASD-like phenotypes in offspring. Together, these results provide novel microbial and epigenetic insights into ASD-related risk and offering a plausible mechanistic explanation for the clinical phenomenon in which unaffected parents give rise to children with ASD.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148227097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut commensal Odoribacter splanchnicus attenuates hyperlipidemic periodontitis via gut-oral metabolic transmission of β-GPA. 肠道共生内脏臭杆菌通过肠道-口腔代谢传递β-GPA减轻高脂血症牙周炎。

IF 11.4 1区生物学

npj Biofilms and Microbiomes Pub Date : 2026-06-11 DOI: 10.1038/s41522-026-01033-4

Jing Xu, Ziyi Han, Qing Xue, Haoran Wang, Yutong Li, Jiyu Song, Lanzhou Li, Min Hu, Di Wang

{"title":"Gut commensal Odoribacter splanchnicus attenuates hyperlipidemic periodontitis via gut-oral metabolic transmission of β-GPA.","authors":"Jing Xu, Ziyi Han, Qing Xue, Haoran Wang, Yutong Li, Jiyu Song, Lanzhou Li, Min Hu, Di Wang","doi":"10.1038/s41522-026-01033-4","DOIUrl":"10.1038/s41522-026-01033-4","url":null,"abstract":"Hyperlipidemic periodontitis (HPD) represents a prevalent comorbidity linking systemic metabolic dysregulation with local inflammation, yet the microbial mechanisms driving this gut-oral crosstalk remain elusive. Here, the comorbid state of HPD is linked to hyperlipidemia-associated gut microbiota changes, which are prominently accompanied by the depletion of Odoribacter splanchnicus in both patients and mice. Fecal microbiota transplantation demonstrates that this gut dysbiosis exacerbates periodontal destruction when local inflammation is present. Mechanistically, intragastric administration of live O. splanchnicus ameliorates HPD by remodeling the gut ecosystem and upregulating the metabolite β-guanidinopropionic acid (β-GPA). Notably, direct supplementation with β-GPA reproduces these protective effects. Furthermore, β-GPA is proposed as a systemic effector linking the gut and periodontal tissues, where its protective effect is associated with the suppression of the pro-inflammatory Toll-like receptor 4 (TLR4) signaling cascade. These findings highlight a link involving O. splanchnicus, β-GPA, and the modulation of TLR4 signaling, offering a potential microbiome-based therapeutic strategy for managing complex metabolic-inflammatory comorbidities.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":" ","pages":""},"PeriodicalIF":11.4,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13601572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148227085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0