Non-Coding RNA最新文献_第4页

Dynamic Localization of Paraspeckle Components under Osmotic Stress. 渗透胁迫下副颈组件的动态定位

IF 4.3

Non-Coding RNA Pub Date : 2024-04-12 DOI: 10.3390/ncrna10020023

Aysegul Yucel-Polat, D. Campos-Melo, Asieh Alikhah, Michael J. Strong

{"title":"Dynamic Localization of Paraspeckle Components under Osmotic Stress.","authors":"Aysegul Yucel-Polat, D. Campos-Melo, Asieh Alikhah, Michael J. Strong","doi":"10.3390/ncrna10020023","DOIUrl":"https://doi.org/10.3390/ncrna10020023","url":null,"abstract":"Paraspeckles are nuclear condensates formed by NEAT1_2 lncRNA and different RNA-binding proteins. In general, these membraneless organelles function in the regulation of gene expression and translation and in miRNA processing, and in doing this, they regulate cellular homeostasis and mediate pro-survival in the cell. Despite evidence showing the importance of paraspeckles in the stress response, the dynamics of paraspeckles and their components under conditions of osmotic stress remain unknown. We exposed HEK293T cells to sorbitol and examined NEAT1_2 expression using real-time PCR. Localization and quantification of the main paraspeckle components, NEAT1_2, PSPC1, NONO, and SFPQ, in different cellular compartments was performed using smFISH and immunofluorescence. Our findings showed a significant decrease in total NEAT1_2 expression in cells after osmotic stress. Sorbitol shifted the subcellular localization of NEAT1_2, PSPC1, NONO, and SFPQ from the nucleus to the cytoplasm and decreased the number and size of NEAT1_2 foci in the nucleus. PSPC1 formed immunoreactive cytoplasmic fibrils under conditions of osmotic stress, which slowly disassembled under recovery. Our study deepens the paraspeckle dynamics in response to stress, suggesting a novel role for NEAT1_2 in the cytoplasm in osmotic stress and physiological conditions.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140711620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Significance of miR-4693-5p in Targeting HIF1α and Its Link to Rheumatoid Arthritis Pathogenesis. miR-4693-5p 靶向 HIF1α 的功能意义及其与类风湿关节炎发病机制的联系

IF 4.3

Non-Coding RNA Pub Date : 2024-04-10 DOI: 10.3390/ncrna10020022

Mohd Saquib, P. Agnihotri, Ashish Sarkar, Swati Malik, Sonia Mann, Debolina Chakraborty, Lovely Joshi, Rajesh Malhotra, S. Biswas

{"title":"Functional Significance of miR-4693-5p in Targeting HIF1α and Its Link to Rheumatoid Arthritis Pathogenesis.","authors":"Mohd Saquib, P. Agnihotri, Ashish Sarkar, Swati Malik, Sonia Mann, Debolina Chakraborty, Lovely Joshi, Rajesh Malhotra, S. Biswas","doi":"10.3390/ncrna10020022","DOIUrl":"https://doi.org/10.3390/ncrna10020022","url":null,"abstract":"Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that causes joint inflammation and destruction with an unknown origin. Our study aims to elucidate the molecular mechanism behind HIF1α overexpression in RA. Dysregulated miRNA expressions are known to influence gene behavior, thereby enhancing cell proliferation, inflammation, and resistance to apoptosis, contributing to RA development. Our earlier finding indicated that exogenous miRNA similar to miR-4693-5p may modulate RA-related targets. However, the specific role of miR-4693-5p and its targets in RA remain unexplored. In this study, we found that miR-4693-5p was significantly reduced in PBMCs of RA patients, with evidence suggesting it targets the 3' UTR of HIF1α, thereby potentially contributing to its overexpression in RA. In vitro overexpression of miR-4693-5p leads to the knockdown of HIF1α, resulting in inhibited expression of Survivin to disrupt apoptosis resistance, inflammation suppression, and a reduction in the total cellular ROS response in SW982 and RAFLS cells. The results were validated using the CIA Rat model. In conclusion, this study provides a crucial foundation for understanding the functional role of miR-4693-5p. These findings improve our understanding and provide novel insights into the molecular mechanisms underlying RA pathogenesis.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140719647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More than the SRY: The Non-Coding Landscape of the Y Chromosome and Its Importance in Human Disease. 超越 SRY：Y 染色体的非编码结构及其在人类疾病中的重要性》（More than the SRY: The Non-Coding Landscape of the Y Chromosome and Its Importance in Human Disease）。

IF 4.3

Non-Coding RNA Pub Date : 2024-04-10 DOI: 10.3390/ncrna10020021

Emily S. Westemeier-Rice, Michael T. Winters, Travis W. Rawson, Ivan Martinez

引用次数: 0

FuncPEP v2.0: An Updated Database of Functional Short Peptides Translated from Non-Coding RNAs. FuncPEP v2.0：从非编码 RNA 翻译而来的功能性短肽的最新数据库。

IF 4.3

Non-Coding RNA Pub Date : 2024-04-09 DOI: 10.3390/ncrna10020020

Swati Mohapatra, Anik Banerjee, Paola C. Rausseo, M. Dragomir, G. Manyam, B. Broom, G. Calin

{"title":"FuncPEP v2.0: An Updated Database of Functional Short Peptides Translated from Non-Coding RNAs.","authors":"Swati Mohapatra, Anik Banerjee, Paola C. Rausseo, M. Dragomir, G. Manyam, B. Broom, G. Calin","doi":"10.3390/ncrna10020020","DOIUrl":"https://doi.org/10.3390/ncrna10020020","url":null,"abstract":"Over the past decade, there have been reports of short novel functional peptides (less than 100 aa in length) translated from so-called non-coding RNAs (ncRNAs) that have been characterized using mass spectrometry (MS) and large-scale proteomics studies. Therefore, understanding the bivalent functions of some ncRNAs as transcripts that encode both functional RNAs and short peptides, which we named ncPEPs, will deepen our understanding of biology and disease. In 2020, we published the first database of functional peptides translated from non-coding RNAs-FuncPEP. Herein, we have performed an update including the newly published ncPEPs from the last 3 years along with the categorization of host ncRNAs. FuncPEP v2.0 contains 152 functional ncPEPs, out of which 40 are novel entries. A PubMed search from August 2020 to July 2023 incorporating specific keywords was performed and screened for publications reporting validated functional peptides derived from ncRNAs. We did not observe a significant increase in newly discovered functional ncPEPs, but a steady increase. The novel identified ncPEPs included in the database were characterized by a wide array of molecular and physiological parameters (i.e., types of host ncRNA, species distribution, chromosomal density, distribution of ncRNA length, identification methods, molecular weight, and functional distribution across humans and other species). We consider that, despite the fact that MS can now easily identify ncPEPs, there still are important limitations in proving their functionality.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140727238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functions of RNAi Pathways in Ribosomal RNA Regulation 核糖体 RNA 调控中的 RNAi 途径功能

IF 4.3

Non-Coding RNA Pub Date : 2024-03-29 DOI: 10.3390/ncrna10020019

A. S. Shatskikh, E. Fefelova, M. Klenov

引用次数: 0

Circulating miRNAs as Novel Clinical Biomarkers in Temporal Lobe Epilepsy. 循环 miRNAs 作为颞叶癫痫的新型临床生物标记物。

IF 4.3

Non-Coding RNA Pub Date : 2024-03-17 DOI: 10.3390/ncrna10020018

Lorenza Guarnieri, Nicola Amodio, Francesca Bosco, Sara Carpi, Martina Tallarico, Luca Gallelli, Vincenzo Rania, Rita Citraro, Antonio Leo, Giovambattista De Sarro

{"title":"Circulating miRNAs as Novel Clinical Biomarkers in Temporal Lobe Epilepsy.","authors":"Lorenza Guarnieri, Nicola Amodio, Francesca Bosco, Sara Carpi, Martina Tallarico, Luca Gallelli, Vincenzo Rania, Rita Citraro, Antonio Leo, Giovambattista De Sarro","doi":"10.3390/ncrna10020018","DOIUrl":"10.3390/ncrna10020018","url":null,"abstract":"Temporal lobe epilepsy (TLE) represents the most common form of refractory focal epilepsy. The identification of innovative clinical biomarkers capable of categorizing patients with TLE, allowing for improved treatment and outcomes, still represents an unmet need. Circulating microRNAs (c-miRNAs) are short non-coding RNAs detectable in body fluids, which play crucial roles in the regulation of gene expression. Their characteristics, including extracellular stability, detectability through non-invasive methods, and responsiveness to pathological changes and/or therapeutic interventions, make them promising candidate biomarkers in various disease settings. Recent research has investigated c-miRNAs in various bodily fluids, including serum, plasma, and cerebrospinal fluid, of TLE patients. Despite some discrepancies in methodologies, cohort composition, and normalization strategies, a common dysregulated signature of c-miRNAs has emerged across different studies, providing the basis for using c-miRNAs as novel biomarkers for TLE patient management.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10961783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting MicroRNAs with Small Molecules. 用小分子靶向 MicroRNA。

IF 4.3

Non-Coding RNA Pub Date : 2024-03-14 DOI: 10.3390/ncrna10020017

Kisanet Tadesse, Raphael I Benhamou

引用次数: 0

miR-125 in Breast Cancer Etiopathogenesis: An Emerging Role as a Biomarker in Differential Diagnosis, Regenerative Medicine, and the Challenges of Personalized Medicine. 乳腺癌发病机制中的 miR-125：miR-125 在乳腺癌发病机制中的作用：作为生物标志物在鉴别诊断、再生医学和个性化医学挑战中的新兴角色。

IF 4.3

Non-Coding RNA Pub Date : 2024-02-21 DOI: 10.3390/ncrna10020016

Roberto Piergentili, Enrico Marinelli, Gaspare Cucinella, Alessandra Lopez, Gabriele Napoletano, Giuseppe Gullo, Simona Zaami

{"title":"miR-125 in Breast Cancer Etiopathogenesis: An Emerging Role as a Biomarker in Differential Diagnosis, Regenerative Medicine, and the Challenges of Personalized Medicine.","authors":"Roberto Piergentili, Enrico Marinelli, Gaspare Cucinella, Alessandra Lopez, Gabriele Napoletano, Giuseppe Gullo, Simona Zaami","doi":"10.3390/ncrna10020016","DOIUrl":"10.3390/ncrna10020016","url":null,"abstract":"Breast Cancer (BC) is one of the most common cancer types worldwide, and it is characterized by a complex etiopathogenesis, resulting in an equally complex classification of subtypes. MicroRNA (miRNA or miR) are small non-coding RNA molecules that have an essential role in gene expression and are significantly linked to tumor development and angiogenesis in different types of cancer. Recently, complex interactions among coding and non-coding RNA have been elucidated, further shedding light on the complexity of the roles these molecules fulfill in cancer formation. In this context, knowledge about the role of miR in BC has significantly improved, highlighting the deregulation of these molecules as additional factors influencing BC occurrence, development and classification. A considerable number of papers has been published over the past few years regarding the role of miR-125 in human pathology in general and in several types of cancer formation in particular. Interestingly, miR-125 family members have been recently linked to BC formation as well, and complex interactions (competing endogenous RNA networks, or ceRNET) between this molecule and target mRNA have been described. In this review, we summarize the state-of-the-art about research on this topic.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10961778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topographic Distribution of miRNAs (miR-30a, miR-223, miR-let-7a, miR-let-7f, miR-451, and miR-486) in the Plasma Extracellular Vesicles 血浆细胞外小泡中 miRNA（miR-30a、miR-223、miR-let-7a、miR-let-7f、miR-451 和 miR-486）的地形分布图

IF 4.3

Non-Coding RNA Pub Date : 2024-02-15 DOI: 10.3390/ncrna10010015

Tatiana Petrova, Olga Kalinina, Arthur Aquino, E. Grigoryev, N. Dubashynskaya, Kseniya Zubkova, Anna Kostareva, A. Golovkin

{"title":"Topographic Distribution of miRNAs (miR-30a, miR-223, miR-let-7a, miR-let-7f, miR-451, and miR-486) in the Plasma Extracellular Vesicles","authors":"Tatiana Petrova, Olga Kalinina, Arthur Aquino, E. Grigoryev, N. Dubashynskaya, Kseniya Zubkova, Anna Kostareva, A. Golovkin","doi":"10.3390/ncrna10010015","DOIUrl":"https://doi.org/10.3390/ncrna10010015","url":null,"abstract":"There are many articles on the quantitative analysis of miRNAs contained in a population of EVs of different sizes under various physiological and pathological conditions. For such analysis, it is important to correctly quantify the miRNA contents of EVs. It should be considered that quantification is skewed depending on the isolation protocol, and different miRNAs are degraded by nucleases with different efficiencies. In addition, it is important to consider the contribution of miRNAs coprecipitating with the EVs population, because the amount of miRNAs in the EVs population under study is skewed without appropriate enzymatic treatment. By studying a population of EVs from the blood plasma of healthy donors, we found that the absolute amount of miRNA inside the vesicles is commensurate with the amount of the same type of miRNA adhered to the outside of the EVs. The inside/outside ratio ranged from 1.02 to 2.64 for different investigated miRNAs. According to our results, we propose the hypothesis that high occupancy of miRNAs on the outer surface of EVs influence on the transporting RNA repertoire no less than the inner cargo received from the host cell.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topographic Distribution of miRNAs (miR-30a, miR-223, miR-let-7a, miR-let-7f, miR-451, and miR-486) in the Plasma Extracellular Vesicles 血浆细胞外小泡中 miRNA（miR-30a、miR-223、miR-let-7a、miR-let-7f、miR-451 和 miR-486）的地形分布图

IF 4.3

Non-Coding RNA Pub Date : 2024-02-15 DOI: 10.3390/ncrna10010015

Tatiana Petrova, Olga Kalinina, Arthur Aquino, E. Grigoryev, N. Dubashynskaya, Kseniya Zubkova, Anna Kostareva, A. Golovkin

{"title":"Topographic Distribution of miRNAs (miR-30a, miR-223, miR-let-7a, miR-let-7f, miR-451, and miR-486) in the Plasma Extracellular Vesicles","authors":"Tatiana Petrova, Olga Kalinina, Arthur Aquino, E. Grigoryev, N. Dubashynskaya, Kseniya Zubkova, Anna Kostareva, A. Golovkin","doi":"10.3390/ncrna10010015","DOIUrl":"https://doi.org/10.3390/ncrna10010015","url":null,"abstract":"There are many articles on the quantitative analysis of miRNAs contained in a population of EVs of different sizes under various physiological and pathological conditions. For such analysis, it is important to correctly quantify the miRNA contents of EVs. It should be considered that quantification is skewed depending on the isolation protocol, and different miRNAs are degraded by nucleases with different efficiencies. In addition, it is important to consider the contribution of miRNAs coprecipitating with the EVs population, because the amount of miRNAs in the EVs population under study is skewed without appropriate enzymatic treatment. By studying a population of EVs from the blood plasma of healthy donors, we found that the absolute amount of miRNA inside the vesicles is commensurate with the amount of the same type of miRNA adhered to the outside of the EVs. The inside/outside ratio ranged from 1.02 to 2.64 for different investigated miRNAs. According to our results, we propose the hypothesis that high occupancy of miRNAs on the outer surface of EVs influence on the transporting RNA repertoire no less than the inner cargo received from the host cell.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139775391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0