Non-Coding RNA最新文献_第4页

Genetic Loss of miR-205 Causes Increased Mammary Gland Development 基因缺失 miR-205 会导致乳腺发育加快

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Non-Coding RNA Pub Date : 2023-12-31 DOI: 10.3390/ncrna10010004

A. Cataldo, Douglas G. Cheung, John Hagan, Matteo Fassan, Sukhinder Sandhu-Deol, Carlo M. Croce, Gianpiero di Leva, M. Iorio

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Long Non-Coding RNAs (lncRNAs) in Heart Failure: A Comprehensive Review 心力衰竭中的长非编码 RNA（lncRNA）：全面综述

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Non-Coding RNA Pub Date : 2023-12-28 DOI: 10.3390/ncrna10010003

Shambhavi Jha, Vasanth Kanth Thasma Loganathbabu, Kasinathan Kumaran, Gopinath Krishnasamy, Kandasamy Nagarajan Aruljothi

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MiR-4646-5p Acts as a Tumor-Suppressive Factor in Triple Negative Breast Cancer and Targets the Cholesterol Transport Protein GRAMD1B MiR-4646-5p 是三阴性乳腺癌的肿瘤抑制因子，靶向胆固醇转运蛋白 GRAMD1B

IF 4.3

Non-Coding RNA Pub Date : 2023-12-26 DOI: 10.3390/ncrna10010002

Katharina Jonas, Felix Prinz, Manuela Ferracin, Katarina Krajina, Alexander Deutsch, Tobias Madl, B. Rinner, O. Slaby, Christiane Klec, Martin Pichler

{"title":"MiR-4646-5p Acts as a Tumor-Suppressive Factor in Triple Negative Breast Cancer and Targets the Cholesterol Transport Protein GRAMD1B","authors":"Katharina Jonas, Felix Prinz, Manuela Ferracin, Katarina Krajina, Alexander Deutsch, Tobias Madl, B. Rinner, O. Slaby, Christiane Klec, Martin Pichler","doi":"10.3390/ncrna10010002","DOIUrl":"https://doi.org/10.3390/ncrna10010002","url":null,"abstract":"MicroRNAs (miRNAs) are crucial post-transcriptional regulators of gene expression, and their deregulation contributes to many aspects of cancer development and progression. Thus, miRNAs provide insight into oncogenic mechanisms and represent promising targets for new therapeutic approaches. A type of cancer that is still in urgent need of improved treatment options is triple negative breast cancer (TNBC). Therefore, we aimed to characterize a novel miRNA with a potential role in TNBC. Based on a previous study, we selected miR-4646-5p, a miRNA with a still unknown function in breast cancer. We discovered that higher expression of miR-4646-5p in TNBC patients is associated with better survival. In vitro assays showed that miR-4646-5p overexpression reduces growth, proliferation, and migration of TNBC cell lines, whereas inhibition had the opposite effect. Furthermore, we found that miR-4646-5p inhibits the tube formation ability of endothelial cells, which may indicate anti-angiogenic properties. By whole transcriptome analysis, we not only observed that miR-4646-5p downregulates many oncogenic factors, like tumor-promoting cytokines and migration- and invasion-related genes, but were also able to identify a direct target, the GRAM domain-containing protein 1B (GRAMD1B). GRAMD1B is involved in cellular cholesterol transport and its knockdown phenocopied the growth-reducing effects of miR-4646-5p. We thus conclude that GRAMD1B may partly contribute to the diverse tumor-suppressive effects of miR-4646-5p in TNBC.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"26 2","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139156105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequencing Reveals miRNAs Enriched in the Developing Mouse Enteric Nervous System 测序揭示发育中小鼠肠神经系统中丰富的 miRNAs

IF 4.3

Non-Coding RNA Pub Date : 2023-12-22 DOI: 10.3390/ncrna10010001

Christopher Pai, Rajarshi Sengupta, R. Heuckeroth

{"title":"Sequencing Reveals miRNAs Enriched in the Developing Mouse Enteric Nervous System","authors":"Christopher Pai, Rajarshi Sengupta, R. Heuckeroth","doi":"10.3390/ncrna10010001","DOIUrl":"https://doi.org/10.3390/ncrna10010001","url":null,"abstract":"The enteric nervous system (ENS) is an essential network of neurons and glia in the bowel wall. Defects in ENS development can result in Hirschsprung disease (HSCR), a life-threatening condition characterized by severe constipation, abdominal distention, bilious vomiting, and failure to thrive. A growing body of literature connects HSCR to alterations in miRNA expression, but there are limited data on the normal miRNA landscape in the developing ENS. We sequenced small RNAs (smRNA-seq) and messenger RNAs (mRNA-seq) from ENS precursor cells of mid-gestation Ednrb-EGFP mice and compared them to aggregated RNA from all other cells in the developing bowel. Our smRNA-seq results identified 73 miRNAs that were significantly enriched and highly expressed in the developing ENS, with miR-9, miR-27b, miR-124, miR-137, and miR-488 as our top 5 miRNAs that are conserved in humans. However, contrary to prior reports, our follow-up analyses of miR-137 showed that loss of Mir137 in Nestin-cre, Wnt1-cre, Sox10-cre, or Baf53b-cre lineage cells had no effect on mouse survival or ENS development. Our data provide important context for future studies of miRNAs in HSCR and other ENS diseases and highlight open questions about facility-specific factors in development.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"53 8","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139164178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The Non-Coding RNA Journal Club: Highlights on Recent Papers-13. 非编码 RNA 期刊俱乐部：近期论文集锦-13。

IF 4.3

Non-Coding RNA Pub Date : 2023-12-14 DOI: 10.3390/ncrna9060076

Patrick K T Shiu, Johanna K DiStefano, Suresh K Alahari, Francisco J Enguita, Mark W Feinberg, Nikolaos Sideris, Salih Bayraktar, Leandro Castellano, Diana Luna Buitrago, Andrea Caporali, Alessandro Mannucci, Ajay Goel

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Regulation of Macrophage Polarization in Allergy by Noncoding RNAs. 非编码 RNA 对过敏症中巨噬细胞极化的调控

IF 4.3

Non-Coding RNA Pub Date : 2023-12-11 DOI: 10.3390/ncrna9060075

Osamu Ishibashi, Stefan A Muljo, Zohirul Islam

{"title":"Regulation of Macrophage Polarization in Allergy by Noncoding RNAs.","authors":"Osamu Ishibashi, Stefan A Muljo, Zohirul Islam","doi":"10.3390/ncrna9060075","DOIUrl":"10.3390/ncrna9060075","url":null,"abstract":"Allergy is a type 2 immune reaction triggered by antigens known as allergens, including food and environmental substances such as peanuts, plant pollen, fungal spores, and the feces and debris of mites and insects. Macrophages are myeloid immune cells with phagocytic abilities that process exogenous and endogenous antigens. Upon activation, they can produce effector molecules such as cytokines as well as anti-inflammatory molecules. The dysregulation of macrophage function can lead to excessive type 1 inflammation as well as type 2 inflammation, which includes allergic reactions. Thus, it is important to better understand how macrophages are regulated in the pathogenesis of allergies. Emerging evidence highlights the role of noncoding RNAs (ncRNAs) in macrophage polarization, which in turn can modify the pathogenesis of various immune-mediated diseases, including allergies. This review summarizes the current knowledge regarding this topic and considers three classes of ncRNAs: microRNAs, long ncRNAs, and circular ncRNAs. Understanding the roles of these ncRNAs in macrophage polarization will provide new insights into the pathogenesis of allergies and identify potential novel therapeutic targets.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10745746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Age and 17β-Estradiol (E2) Facilitate Nuclear Export and Argonaute Loading of microRNAs in the Female Brain 年龄和 17β 雌二醇 (E2) 促进雌性大脑中 microRNA 的核输出和 Argonaute 装载

IF 4.3

Non-Coding RNA Pub Date : 2023-12-06 DOI: 10.3390/ncrna9060074

Megan L. Linscott, Yoldas Yildiz, Sarah Flury, Mikayla L. Newby, Toni R. Pak

{"title":"Age and 17β-Estradiol (E2) Facilitate Nuclear Export and Argonaute Loading of microRNAs in the Female Brain","authors":"Megan L. Linscott, Yoldas Yildiz, Sarah Flury, Mikayla L. Newby, Toni R. Pak","doi":"10.3390/ncrna9060074","DOIUrl":"https://doi.org/10.3390/ncrna9060074","url":null,"abstract":"Aging in women is accompanied by a dramatic change in circulating sex steroid hormones. Specifically, the primary circulating estrogen, 17β-estradiol (E2), is nearly undetectable in post-menopausal women. This decline is associated with a variety of cognitive and mood disorders, yet hormone replacement therapy is only effective within a narrow window of time surrounding the menopausal transition. Our previous work identified microRNAs as a potential molecular substrate underlying the change in E2 efficacy associated with menopause in advanced age. Specifically, we showed that E2 regulated a small subset of mature miRNAs in the aging female brain. In this study, we hypothesized that E2 regulates the stability of mature miRNAs by altering their subcellular localization and their association with argonaute proteins. We also tested the hypothesis that the RNA binding protein, hnRNP A1, was an important regulator of mature miR-9-5p expression in neuronal cells. Our results demonstrated that E2 treatment affected miRNA subcellular localization and its association with argonaute proteins differently, depending on the length of time following E2 deprivation (i.e., ovariectomy). We also provide strong evidence that hnRNP A1 regulates the transcription of pri-miR-9 and likely plays a posttranscriptional role in mature miR-9-5p turnover. Taken together, these data have important implications for considering the optimal timing for hormone replacement therapy, which might be less dependent on age and more related to how long treatment is delayed following menopause.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"43 15","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138597548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs in Gingival Crevicular Fluid: An Observational Case-Control Study of Differential Expression in Periodontitis. 龈沟液中的microrna:牙周炎患者差异表达的观察性病例对照研究。

IF 4.3

Non-Coding RNA Pub Date : 2023-11-18 DOI: 10.3390/ncrna9060073

Pedro J Almiñana-Pastor, Francisco M Alpiste-Illueca, Pablo Micó-Martinez, Jose Luis García-Giménez, Eva García-López, Andrés López-Roldán

{"title":"MicroRNAs in Gingival Crevicular Fluid: An Observational Case-Control Study of Differential Expression in Periodontitis.","authors":"Pedro J Almiñana-Pastor, Francisco M Alpiste-Illueca, Pablo Micó-Martinez, Jose Luis García-Giménez, Eva García-López, Andrés López-Roldán","doi":"10.3390/ncrna9060073","DOIUrl":"10.3390/ncrna9060073","url":null,"abstract":"Objectives: microRNAs (miRNAs) present in the gingival crevicular fluid (GCF) of patients with chronic periodontitis may serve as biomarkers of periodontal disease. The aim of this study was to perform a miRNA-sequencing study of all miRNAs present in GCF, comparing miRNA expression level profiles between advanced chronic periodontitis (CP) patients and healthy subjects (HS).Materials and methods: GCF samples were collected from the single-rooted teeth of patients with severe CP (n = 11) and of HS (n = 12). miRNAs were isolated from GCF using an miRNeasy Serum/Plasma kit(Qiagen GmbH, Hilden, Germany). Reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression levels of miRNA candidates involved in periodontal pathogenesis.Results: Of all the sequenced miRNAs, miR-199, miR-146a, miR-30a, and miR-338 were identified as best representing the CP patient samples. The validation study identified miR-199 as the most powerful biomarker used to define periodontitis.Conclusions: Upon sequencing all known miRNAs in GCF for the first time, we uncovered several potential biomarkers to define periodontitis. Identifying miRNAS in the GCF using high-throughput approaches will clarify the role of these molecules in periodontitis and provide biomarkers with potential applications.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons. 乙醇和parp介导的核糖体相关长链非编码RNA (lncRNA)在锥体神经元中的调控。

IF 4.3

Non-Coding RNA Pub Date : 2023-11-17 DOI: 10.3390/ncrna9060072

Hooriyah S Rizavi, Hannah E Gavin, Harish R Krishnan, David P Gavin, Rajiv P Sharma

{"title":"Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons.","authors":"Hooriyah S Rizavi, Hannah E Gavin, Harish R Krishnan, David P Gavin, Rajiv P Sharma","doi":"10.3390/ncrna9060072","DOIUrl":"10.3390/ncrna9060072","url":null,"abstract":"Although, by definition, long noncoding RNAs (lncRNAs) are not translated, they are sometimes associated with ribosomes. In fact, some estimates suggest the existence of more than 50 K lncRNA molecules that could encode for small peptides. We examined the effects of an ethanol and Poly-ADP Ribose Polymerase (PARP) inhibitor (ABT-888) on ribosome-bound lncRNAs. Mice were administered via intraperitoneal injection (i.p.) either normal saline (CTL) or ethanol (EtOH) twice a day for four consecutive days. On the fourth day, a sub-group of mice administered with ethanol also received ABT-888 (EtOH+ABT). Ribosome-bound lncRNAs in CaMKIIα-expressing pyramidal neurons were measured using the Translating Ribosome Affinity Purification (TRAP) technique. Our findings show that EtOH altered the attachment of 107 lncRNA transcripts, while EtOH+ABT altered 60 lncRNAs. Among these 60 lncRNAs, 49 were altered by both conditions, while EtOH+ABT uniquely altered the attachment of 11 lncRNA transcripts that EtOH alone did not affect. To validate these results, we selected eight lncRNAs (Mir124-2hg, 5430416N02Rik, Snhg17, Snhg12, Snhg1, Mir9-3hg, Gas5, and 1110038B12Rik) for qRT-PCR analysis. The current study demonstrates that ethanol-induced changes in lncRNA attachment to ribosomes can be mitigated by the addition of the PARP inhibitor ABT-888.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LADON, a Natural Antisense Transcript of NODAL, Promotes Tumour Progression and Metastasis in Melanoma. LADON是一种天然的NODAL反义转录物，促进黑色素瘤的肿瘤进展和转移。

IF 4.3

Non-Coding RNA Pub Date : 2023-11-15 DOI: 10.3390/ncrna9060071

Annie Dutriaux, Serena Diazzi, Chiara Bresesti, Sylvie Hardouin, Frédérique Deshayes, Jérôme Collignon, Domenico Flagiello

{"title":"LADON, a Natural Antisense Transcript of NODAL, Promotes Tumour Progression and Metastasis in Melanoma.","authors":"Annie Dutriaux, Serena Diazzi, Chiara Bresesti, Sylvie Hardouin, Frédérique Deshayes, Jérôme Collignon, Domenico Flagiello","doi":"10.3390/ncrna9060071","DOIUrl":"10.3390/ncrna9060071","url":null,"abstract":"The TGFβ family member NODAL, repeatedly required during embryonic development, has also been associated with tumour progression. Our aim was to clarify the controversy surrounding its involvement in melanoma tumour progression. We found that the deletion of the NODAL exon 2 in a metastatic melanoma cell line impairs its ability to form tumours and colonize distant tissues. However, we show that this phenotype does not result from the absence of NODAL, but from a defect in the expression of a natural antisense transcript of NODAL, here called LADON. We show that LADON expression is specifically activated in metastatic melanoma cell lines, that its transcript is packaged in exosomes secreted by melanoma cells, and that, via its differential impact on the expression of oncogenes and tumour suppressors, it promotes the mesenchymal to amoeboid transition that is critical for melanoma cell invasiveness. LADON is, therefore, a new player in the regulatory network governing tumour progression in melanoma and possibly in other types of cancer.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0