Small RNA Landscape of Platelet Dust: Platelet-Derived Extracellular Vesicles from Patients with Non-Small-Cell Lung Cancer.

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Non-Coding RNA Pub Date : 2025-05-07 DOI:10.3390/ncrna11030038

Mafalda Antunes-Ferreira, Ilias Glogovitis, Diogo Fortunato, Silvia D'Ambrosi, Mariona Colom Saborit, Galina Yahubyan, Vesselin Baev, Michael Hackenberg, Natasa Zarovni, Thomas Wurdinger, Danijela Koppers-Lalic

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引用次数: 0

Abstract

Background: Platelet-derived Extracellular Vesicles, or "Platelet Dust" (PD), are reported as the most-abundant extracellular vesicles in plasma. However, the PD molecular content, especially the small RNA profile, is still poorly characterized. This study aims to characterize PD and other extracellular vesicles (EVs) in patients with non-small-cell lung cancer (NSCLC), focusing on their small RNA signatures and diagnostic potential. Methods: The EVs were isolated directly from the plasma of healthy donors and patients with NSCLC using the surface markers CD9, CD63, CD81 (overall EVs), and CD61 (PD). Small RNA sequencing was then performed to comprehensively profile the miRNAs. Results: Our analysis revealed distinct small RNA profiles in the EVs and the PD from the patients with NSCLC. The EVs (CD9-, CD63-, and CD81-positive) showed the enrichment of four miRNAs and the depletion of ten miRNAs, while the PD (CD61-positive) exhibited a more complex profile, with nineteen miRNAs enriched and nine miRNAs depleted in the patients with NSCLC compared to those of the healthy controls. Conclusions: This exploratory study enhances our understanding of miRNA composition within different plasma vesicle populations, shedding light on the biology of plasma vesicles and their contents. Furthermore, utilizing an extracellular vesicle isolation method with potential clinical applicability offers the prospect of improved cancer characterization and detection by selecting the most informative subpopulation of plasma vesicles.

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血小板尘埃的小RNA景观：来自非小细胞肺癌患者的血小板来源的细胞外囊泡。

背景：血小板来源的细胞外囊泡，或“血小板尘埃”（PD），被报道为血浆中最丰富的细胞外囊泡。然而，PD分子含量，特别是小RNA谱，仍然缺乏表征。本研究旨在表征PD和非小细胞肺癌（NSCLC）患者的其他细胞外囊泡（ev），重点研究它们的小RNA特征和诊断潜力。方法：采用表面标记CD9、CD63、CD81（总ev）和CD61 （PD）直接从健康供者和非小细胞肺癌患者的血浆中分离ev。然后进行小RNA测序以全面分析mirna。结果：我们的分析显示，在非小细胞肺癌患者的ev和PD中存在不同的小RNA谱。EVs （CD9-、CD63-和cd81阳性）表现出4种mirna的富集和10种mirna的缺失，而PD （cd61阳性）表现出更复杂的特征，与健康对照组相比，NSCLC患者中有19种mirna富集，9种mirna缺失。结论：本探索性研究增强了我们对不同血浆囊泡群体中miRNA组成的理解，揭示了血浆囊泡及其含量的生物学特性。此外，利用具有潜在临床适用性的细胞外囊泡分离方法，通过选择信息最丰富的血浆囊泡亚群，为改进癌症表征和检测提供了前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

6.70

自引率

4.70%

发文量

审稿时长

10 weeks

期刊介绍： Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.