NeurosciencePub Date : 2025-02-26DOI: 10.1016/j.neuroscience.2025.02.052
Xinyu Tao , Juyi Zhang , Jiangang Liu , Feng Gu , Longyuan Li , Xin Wu , Kun Dai , Haitao Shen , Xiang Li , Haiying Li , Zongqi Wang , Zhong Wang
{"title":"SARM1 Modulates calcium influx in secondary brain injury after experimental Intracerebral hemorrhage","authors":"Xinyu Tao , Juyi Zhang , Jiangang Liu , Feng Gu , Longyuan Li , Xin Wu , Kun Dai , Haitao Shen , Xiang Li , Haiying Li , Zongqi Wang , Zhong Wang","doi":"10.1016/j.neuroscience.2025.02.052","DOIUrl":"10.1016/j.neuroscience.2025.02.052","url":null,"abstract":"<div><div>Intracerebral hemorrhage (ICH), defined as spontaneous bleeding within brain tissue, is associated with high mortality and severe disability, often resulting in poor clinical outcomes. Early intervention to mitigate secondary brain injury is critical for neuronal protection. Sterile alpha and Toll/interleukin-1 receptor motif-containing 1 (SARM1), a member of the MyD88 family, is predominantly expressed in neurons, where it localizes to the outer membrane of mitochondria. Under physiological conditions, SARM1 is expressed at low levels; however, its expression increases following injury, resulting in excessive NAD<sup>+</sup> hydrolysis. While NAD<sup>+</sup> degradation products can influence calcium channels, their role in calcium regulation after ICH remains unclear. This study established an <em>in vivo</em> ICH model in adult SD rats via autologous blood injection into the basal ganglia and validated the findings using an <em>in vitro</em> model of primary neurons treated with oxyhemoglobin. SARM1 knockdown was achieved using a lentiviral vector. Following ICH, SARM1 expression significantly increased and colocalized with the neuronal marker NeuN. SARM1 knockdown reduced both SARM1 and mitochondrial calcium uniporter (MCU) expression, decreased NAD<sup>+</sup> degradation, and attenuated neuronal death. Behavioral assessments demonstrated improved short- and long-term neurological outcomes in SARM1-knockdown rats compared with the lentiviral vector group. In <em>in vitro</em> experiments, Rhod-2 staining revealed reduced mitochondrial calcium levels, while TMRM staining indicated decreased mitochondrial membrane potential loss. Additionally, Hoechst staining showed reduced neuronal mitochondrial death following SARM1 downregulation. These findings suggest that targeting SARM1 may enhance neurological recovery and represents a potential therapeutic strategy for early intervention in secondary brain injury following ICH.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"571 ","pages":"Pages 32-43"},"PeriodicalIF":2.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NeurosciencePub Date : 2025-02-26DOI: 10.1016/j.neuroscience.2025.02.054
Li Wu , Chunmei Pang , Guanfeng Zeng , Dezhi Liu , Chenghao Li , Kang Liu , Pengyue Huang , Wenbo Wang , Juehua Zhu , Zheng Dai , Zhihua Qiu , Yongjun Jiang
{"title":"10-year trajectories of triglyceride-glucose index and progression of vertebrobasilar artery stenosis: A multicenter hospital-based prospective longitudinal cohort study","authors":"Li Wu , Chunmei Pang , Guanfeng Zeng , Dezhi Liu , Chenghao Li , Kang Liu , Pengyue Huang , Wenbo Wang , Juehua Zhu , Zheng Dai , Zhihua Qiu , Yongjun Jiang","doi":"10.1016/j.neuroscience.2025.02.054","DOIUrl":"10.1016/j.neuroscience.2025.02.054","url":null,"abstract":"<div><h3>Background</h3><div>The association between the triglyceride-glucose (TyG) index and the atherosclerosis has been validated by numerous evidences. However, the prospective relationship between long-term dynamic changes in the TyG index and the progression of vertebrobasilar artery (VBA) atherosclerotic plaques remained unclear.</div></div><div><h3>Methods</h3><div>This multicenter, hospital-based, prospective longitudinal cohort study included 1,336 patients with suspected stroke from January 1, 2004 to December 31, 2022. Baseline characteristics and vascular examinations were collected and performed at baseline. Patients were followed up for 10 years. The latent class trajectory modeling method was used to analyze the TyG index trajectories over the follow-up period. Cox regression was used to analyze the association of the baseline and trajectory of the TyG index with the progression of VBA plaques.</div></div><div><h3>Results</h3><div>During the follow-up (10.80 ± 2.30 years), VBA plaque progression was found in 175 participants. Cox regression analysis indicated there was a significant positive association between the baseline TyG index and the VBA plaque progression (HR 1.886, 95 % CI 1.166–3.050, <em>P</em> = 0.010). Two trajectories of the TyG index were identified in a total of 1,336 participants, the low-stable group and high-increasing group. Compared with the low-stable group, the odds ratio for the high-increasing group had a 3.156-fold (95 % CI 1.629–6.112, <em>P</em> = 0.001) risk of VBA plaque progression.</div></div><div><h3>Conclusions</h3><div>Our findings suggested that higher baseline and the high-increasing trajectory of the TyG index were associated with VBA plaque progression. People with high-increasing trajectories of the TyG index predispose to develop VBA plaque progression and deserve more attention and more aggressive preventive therapies.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"571 ","pages":"Pages 44-51"},"PeriodicalIF":2.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143528873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NeurosciencePub Date : 2025-02-26DOI: 10.1016/j.neuroscience.2025.02.053
Gauthier Everard , Madeleine Vermette , Elizabeth Dumas-Longpré , Teresa Hoang , Megann Robitaille , Gregorio Sorrentino , Martin Gareth Edwards , Thierry Lejeune , Charles Sebiyo Batcho
{"title":"Self-adaptive over progressive non-adaptive immersive virtual reality serious game to promote motor learning in older adults – A double blind randomized controlled trial","authors":"Gauthier Everard , Madeleine Vermette , Elizabeth Dumas-Longpré , Teresa Hoang , Megann Robitaille , Gregorio Sorrentino , Martin Gareth Edwards , Thierry Lejeune , Charles Sebiyo Batcho","doi":"10.1016/j.neuroscience.2025.02.053","DOIUrl":"10.1016/j.neuroscience.2025.02.053","url":null,"abstract":"<div><div>Older adults often experience cognitive and functional decline, which can be mitigated by innovative interventions like immersive virtual reality (iVR). Self-adaptive serious games, which dynamically adjust task difficulty, may provide additional benefits by tailoring challenges to individual performance. However, the comparative effectiveness of self-adaptive versus progressive non-adaptive iVR interventions on motor learning in older adults remains underexplored. This randomized controlled trial primarily aimed to compare the effects of self-adaptive and progressive non-adaptive iVR interventions on motor learning in older adults. A total of 33 older adults were randomly assigned to either a self-adaptive (n = 17) or progressive non-adaptive (n = 16) iVR group. Both interventions involved an iVR serious game requiring unimanual reaching, target selection, and distractor inhibition under increasing difficulty over three days. Performance was evaluated pre- and post-intervention using a standardized iVR task, consisting of hitting a virtual target while ignoring distractors. This task features five levels of difficulty (level 0: no distractors; level 4: 17 low-saliency distractors). Primary outcomes, assessed during the standardized task, included changes in speed-accuracy trade-offs (SAT) and relative response time (RT, compared to level 0). Both participants and assessors were blinded to the intervention. After the intervention, both groups exhibited significant SAT improvements at level 1, 3 and 4 (P < 0.05) and RT gains at level 3 and 4 (P < 0.05). The self-adaptive group demonstrated significantly greater SAT (P = 0.049) and relative RT (P = 0.004) improvements at level 4. Findings suggest that older adults can achieve motor learning in iVR, particularly with self-adaptive serious games, highlighting potential for self-rehabilitation. Clinical trial NCT06141642 approved the 21th November 2023.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"571 ","pages":"Pages 7-18"},"PeriodicalIF":2.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NeurosciencePub Date : 2025-02-25DOI: 10.1016/j.neuroscience.2025.02.051
Yichuan Wang , Mengting Li , Zexuan Xu , Yilin Wang , Jianxin Zhang , Zeqi Hao , Xize Jia , Dailun Hou
{"title":"Alterations of white matter integrity in patients with intracranial tuberculosis: A tract-based spatial statistics study","authors":"Yichuan Wang , Mengting Li , Zexuan Xu , Yilin Wang , Jianxin Zhang , Zeqi Hao , Xize Jia , Dailun Hou","doi":"10.1016/j.neuroscience.2025.02.051","DOIUrl":"10.1016/j.neuroscience.2025.02.051","url":null,"abstract":"<div><h3>Objective</h3><div>Intracranial tuberculosis has high mortality and morbidity. Its physiopathologic mechanism, especially neurocognitive mechanism, remains unclear, and there are few studies on white matter in such patients. This study aimed to investigate the white matter abnormalities in patients with intracranial tuberculosis using tract-based spatial statistics (TBSS) method of diffusion tensor imaging (DTI).</div></div><div><h3>Methods</h3><div>Fifty patients with intracranial tuberculosis and 49 healthy controls (HCs) were included in this study. Differences in the fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD) between patients and HCs were investigated using TBSS analysis. Partial correlation analysis was performed to explore correlations between DTI values and cognitive functions in patients with intracranial tuberculosis, with age and gender as covariates. Subgroup analysis was also conducted using the same method.</div></div><div><h3>Results</h3><div>Compared to HCs, patients with intracranial tuberculosis showed a widespread reduction of FA and increase of MD, RD and AD (<em>P</em> < 0.05, TFCE and FWE corrected). Notably, abnormalities in all four metrics were observed in 25 white matter tracts according to the Johns Hopkins University ICBM-DTI-81 White Matter Atlas. Patients with mixed intracranial tuberculosis (mixed-ITB) showed increased AD in a limited range compared with patients with tuberculous meningitis (TBM). With age and gender considered, the MD, RD and AD values in some regions significantly correlated with the cognitive assessment scores.</div></div><div><h3>Conclusions</h3><div>The results of this study indicated that patients with intracranial tuberculosis might have widespread abnormalities in the white matter of the brain, which might associate with their cognitive impairment.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"572 ","pages":"Pages 11-20"},"PeriodicalIF":2.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NeurosciencePub Date : 2025-02-23DOI: 10.1016/j.neuroscience.2025.02.043
Wenting Yu , Binn Zhang , Yanan Li , Ying Liu
{"title":"Neurobiological mechanisms underlying coordinated actions in joint action","authors":"Wenting Yu , Binn Zhang , Yanan Li , Ying Liu","doi":"10.1016/j.neuroscience.2025.02.043","DOIUrl":"10.1016/j.neuroscience.2025.02.043","url":null,"abstract":"<div><h3>Background</h3><div>The coordination of actions in joint action significantly impacts various aspects of daily life. Previous research, utilizing parameters derived from behavioral dynamics, revealed that an individual’s jumping behavior is influenced by the proximity of their partner’s jump, implying a potential role of action simulation in interpersonal coordinated actions. This study employs functional near-infrared brain imaging technology to directly investigate the neural mechanisms associated with coordinated actions in joint action.</div></div><div><h3>Method</h3><div>Using a modified joint jumping task, participants were instructed to jump varying distances without observing their partner’s actions, aiming to achieve a collaborative goal of landing simultaneously. Concurrently collecting behavioral parameters related to jumping, we synchronized the acquisition of cerebral hemodynamic data.</div></div><div><h3>Result</h3><div>At the neural activity level, within the motor-related cortex, regardless of whether one jumped closer or farther, this region exhibited higher concentrations of oxygenated hemoglobin compared to the condition with both participants jumping the same distance. In the dorsolateral prefrontal cortex, only when one needed to jump closer did a higher concentration of oxygenated hemoglobin emerge.</div></div><div><h3>Conclusion</h3><div>The dorsolateral prefrontal cortex, associated with action coordination strategies, and the motor-related cortex may be directly linked to action simulation.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"573 ","pages":"Pages 64-73"},"PeriodicalIF":2.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NeurosciencePub Date : 2025-02-22DOI: 10.1016/j.neuroscience.2025.02.038
Ziyang Cui , Junxiong Qiu , Jianwei Lin , Yanni Fu , Liling Lin
{"title":"Discovering genetically-supported drug targets for multisite chronic pain through multi-omics Mendelian randomization and single-cell RNA-sequencing","authors":"Ziyang Cui , Junxiong Qiu , Jianwei Lin , Yanni Fu , Liling Lin","doi":"10.1016/j.neuroscience.2025.02.038","DOIUrl":"10.1016/j.neuroscience.2025.02.038","url":null,"abstract":"<div><div>Multisite chronic pain (MCP) is a highly prevalent disorder with substantial unmet therapeutic needs.We conducted multi-omics Mendelian randomization and Bayesian colocalization to identify potential therapeutic targets for MCP. Summary-level data of gene expressions and protein abundance levels were obtained from corresponding quantitative trait loci studies, respectively. Summary-level data for MCP was leveraged from the UK Biobank. The transcriptome-wide association study (TWAS), Mendelian randomization, and Bayesian colocalization approaches were applied to investigate the potential causal effects of gene expressions and protein levels on MCP in both blood and brain tissues. Phenome-wide Mendelian randomization analysis (MR-PheWAS), single-cell sequencing data, protein–protein interaction (PPI), and reaction pathway analysis were further conducted to digging the underlying mechanisms. Our analysis identified and validated two plasma targets for MCP, namely KLC1 and LANCL1, at both gene expression levels and protein levels across multi-methodologies. Moreover, MR-PheWAS observed additional benefits associated with these two targets. Through analyses based on single-cell sequencing data, we identified critical cell types for KLC1, primarily megakaryocytes, and neurons, notably linked to the axon guidance pathway, while LANCL1 showed associations with B lymphocytes, neurons, and the electron transport pathway. In dorsal root ganglions, we identified enrichments of both LANCL1 and KLC1 in putative silent nociceptors. The effects are possibly mediated through axonal transport and the activation of NMDARs, supported by PPI and reaction pathway analysis. Our multi-dimensional analysis suggests that genetically determined KLC1 and LANCL1 are causally linked to MCP risk, holding promise as appealing drug targets for MCP.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"572 ","pages":"Pages 254-268"},"PeriodicalIF":2.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NeurosciencePub Date : 2025-02-22DOI: 10.1016/j.neuroscience.2025.02.020
Francisco J. Ruiz-Martínez , Manuel Muñoz-Caracuel , Vanesa Muñoz , Ana Gómez Treviño , Carlos M. Gómez
{"title":"Event-Related Spectral Perturbations differences analyzed in standard-deviant tone sequences presented in passive and active conditions","authors":"Francisco J. Ruiz-Martínez , Manuel Muñoz-Caracuel , Vanesa Muñoz , Ana Gómez Treviño , Carlos M. Gómez","doi":"10.1016/j.neuroscience.2025.02.020","DOIUrl":"10.1016/j.neuroscience.2025.02.020","url":null,"abstract":"<div><div>The predictive coding theory, although a well-supported framework for understanding brain processing, remains elusive regarding how different brain rhythms contribute to error prediction and modify the a priori probabilities of predictive events. This study addresses this issue by analyzing Event-Related Spectral Perturbations (ERSP) generated during an auditory oddball paradigm presented in both a passive and active condition. The design involved sequences of four tones, where the last tone was either predictable (standard, S), completing the scale, or less predictable (deviant, D) when the first tone was occasionally repeated. In the passive condition, participants were instructed to ignore the sounds, whereas, in the active condition, they were asked to press the up or down arrow on a keyboard depending on whether the last tone of the sequence presented a higher or lower frequency than the previous one. This experimental design aimed to bias cognitive processing towards predictable (S) or unpredictable scenarios (D) in two different conditions: passive and attentional. EEG data from 13 channels were analyzed with Morlet wavelets, revealing event-related synchronization (ERS) and desynchronization (ERD) induced by the stimuli. Early theta activity was key in computing prediction errors and updating next-trial expectations. In the active condition, theta responses were higher in D than in S trials, indicating enhanced prediction error processing with attention. Early beta activity also increased during D, likely reflecting motor adjustments. These findings emphasize the critical role of early theta rhythms and the amplifying effect of attention on prediction error processing.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"571 ","pages":"Pages 19-30"},"PeriodicalIF":2.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NeurosciencePub Date : 2025-02-22DOI: 10.1016/j.neuroscience.2025.02.049
Xiaorong Zheng , Yuanjia Zheng , Zhe Zhai , Yiwen Chen , Yao Zhu , Guofan Qiu , Bokai Wang , Shuxin Wang , Yongjun Chen , Jinglan Yan
{"title":"Electroacupuncture restores maternal separation-induced glutamatergic presynaptic deficits of the medial prefrontal cortex in adulthood","authors":"Xiaorong Zheng , Yuanjia Zheng , Zhe Zhai , Yiwen Chen , Yao Zhu , Guofan Qiu , Bokai Wang , Shuxin Wang , Yongjun Chen , Jinglan Yan","doi":"10.1016/j.neuroscience.2025.02.049","DOIUrl":"10.1016/j.neuroscience.2025.02.049","url":null,"abstract":"<div><div>Maternal separation (MS) serves as a critical model of early life stress (ELS) that can lead to mood disorders, such as depression. Our previous studies suggest that MS may disrupt synaptic transmission in adulthood. While electroacupuncture (EA) has demonstrated antidepressant effects in several animal models of stress-induced depression, it remains unclear whether EA can reverse synaptic transmission deficits caused by ELS. In this study, we examined the effects of EA at Baihui (GV20) and Yintang (GV29) on both behavioural deficits and glutamatergic synaptic transmission in Sprague-Dawley rats subjected to MS. First, we showed that EA effectively alleviated anhedonia and despair-like behaviours. Furthermore, our data indicated that EA restored the decrease in presynaptic glutamate release, as evidenced by changes in the frequency of miniature excitatory postsynaptic currents (mEPSCs) and paired-pulse ratios (PPR). Microdialysis results also suggested that EA elevated extracellular glutamate levels. To explore the underlying mechanisms, we performed Western blot analyses on several proteins involved in glutamatergic synaptic transmission. Notably, we found that EA treatment increased the expression of vesicular glutamate transporters (VGLUT1 and VGLUT2) and vesicle-associated release proteins (SNAP25, Syntaxin-1A, and VAMP2) in the medial prefrontal cortex (mPFC) of MS rats. In contrast, EA did not significantly affect most postsynaptic glutamatergic receptors. These findings underscore the significant impact of EA on glutamatergic synaptic transmission, particularly in restoring presynaptic impairments induced by MS in adulthood.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"570 ","pages":"Pages 203-212"},"PeriodicalIF":2.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
NeurosciencePub Date : 2025-02-22DOI: 10.1016/j.neuroscience.2025.02.048
Xuanwei Zhang , Wenyu Zhang , Yan Li , Guangda Liang , Shirong Peng , Yi Shen , Xingwei Wu , Kaiyu Nie , Zhi Xiao , Xingfeng Liu
{"title":"Hippocampal P2X4 receptor induces type 1 diabetes rats with neuropathic pain through microglial-derived neuroinflammation and neuronal damage","authors":"Xuanwei Zhang , Wenyu Zhang , Yan Li , Guangda Liang , Shirong Peng , Yi Shen , Xingwei Wu , Kaiyu Nie , Zhi Xiao , Xingfeng Liu","doi":"10.1016/j.neuroscience.2025.02.048","DOIUrl":"10.1016/j.neuroscience.2025.02.048","url":null,"abstract":"<div><div>Diabetic neuropathic pain (DNP) is a serious complication of diabetes, characterized by spontaneous burning pain, hyperalgesia or allodynia, and is associated with severely reduced quality of life. The purinergic P2X4 receptor (P2X4R) plays an essential role in neuropathic pain. In this study, we investigated the roles of hippocampal P2X4R in type 1 diabetes (T1D) rats with DNP. The reduced body weight, elevated blood glucose, and reduced mechanical withdrawal threshold (MWT) were manifested in DNP rats. The increased hippocampal P2X4R enhanced the release of TNF-α, IL-1β, IL-6, which may be related to activated microglia, thereby inducing the development of DNP, and these changes were attenuated by P2X4R antagonist. Our findings suggest that in the state of T1D, hippocampal P2X4R was elevated and enhanced reactive microglia, thereby aggravating the release of pro-inflammatory cytokines and neuronal damage to aggravate hyperalgesia.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"570 ","pages":"Pages 225-235"},"PeriodicalIF":2.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The intrinsic functional connectivity patterns of the phonological and semantic networks in word reading","authors":"Yuan Feng, Shuo Zhang, Aqian Li, Xiaoxue Feng, Rui Hu, Leilei Mei","doi":"10.1016/j.neuroscience.2025.02.050","DOIUrl":"10.1016/j.neuroscience.2025.02.050","url":null,"abstract":"<div><div>Previous studies have revealed that phonological and semantic processing recruit separate brain networks. However, the intrinsic functional connectivity patterns of the phonological and semantic networks remain unclear. To address this issue, the present study explored the static and dynamic functional connectivity patterns of phonological and semantic networks during the resting state. The static functional connectivity pattern of the two networks was examined by adopting a voxel-based global brain connectivity (GBC) method. In this analysis, we estimated the within-network connectivity (WNC), between-network connectivity between phonological and semantic networks (BNC_PS), and between-network connectivity of the two language networks (i.e., phonological and semantic networks) with the non-language network (BNC_N). The results showed that both phonological and semantic networks exhibited stronger intra-network connectivity (i.e., WNC) than inter-network connectivity (i.e., BNC_PS and BNC_N), indicating that both networks are relatively encapsulated. For dynamic functional connectivity, three distinct dynamic functional states were identified. Specifically, State 1 showed an overall positive connectivity pattern. State 2 exhibited an overall weak connectivity pattern. State 3 showed positive intra-network connectivity and negative inter-network connectivity. These results suggested that phonological and semantic networks exhibited a flexible integration and segregation pattern over time. Taken together, our results revealed that the phonological and semantic networks showed an intra-network integration and inter-network segregation pattern. These findings deepen our understanding of the intrinsic functional connectivity patterns of language networks.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"571 ","pages":"Pages 139-150"},"PeriodicalIF":2.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}