Neuroscience最新文献_第10页

Hyperbaric oxygen therapy enhances hippocampal long-term potentiation via upregulation of BDNF and p-CREB1 in rats: Evidence outside pathological models 高压氧治疗通过上调大鼠BDNF和p-CREB1增强海马长期增强：病理模型外的证据

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-25 DOI: 10.1016/j.neuroscience.2025.08.045

Mehmet Akif Baktir , Ozlem Calik , Kemal Erdem Basaran , Buse Gunaydin Turker , Cem Suer

{"title":"Hyperbaric oxygen therapy enhances hippocampal long-term potentiation via upregulation of BDNF and p-CREB1 in rats: Evidence outside pathological models","authors":"Mehmet Akif Baktir , Ozlem Calik , Kemal Erdem Basaran , Buse Gunaydin Turker , Cem Suer","doi":"10.1016/j.neuroscience.2025.08.045","DOIUrl":"10.1016/j.neuroscience.2025.08.045","url":null,"abstract":"<div><div>Hyperbaric oxygen therapy (HBOT) is known to confer neuroprotective benefits in injury models, but its in vivo effects on synaptic plasticity in the healthy brain remain unclear. This study investigates HBOT’s impact on hippocampal long-term potentiation (LTP) and underlying molecular mechanisms in adult male Wistar rats. Animals were randomized into Control (ambient air) and HBOT (100 % O<sub>2</sub> at 2.4 ATA for 1 h daily) groups for seven days. On day 8, field potentials were recorded from the dentate gyrus to generate input–output curves for excitatory postsynaptic potential (EPSP) slope and population spike (PS) amplitude. Time-course responses were then monitored over a 90-minute period using four 100 Hz high-frequency stimulation trains for LTP induction. Following electrophysiology, hippocampal tissue was assayed for pro-BDNF, BDNF, CREB1, and p-CREB1 via immunofluorescence and qPCR. HBOT-treated rats exhibited significantly greater LTP during the maintenance phase: PS amplitude peaked at 233.4 ± 17.9 % versus 130.9 ± 20.1 % in controls (p = 0.0019), and EPSP slope reached 125.0 ± 6.2 % versus 91.6 ± 9.5 % (p = 0.0104). Immunofluorescence revealed elevated pro-BDNF (p = 0.0058), p-CREB1 (p = 0.0011), and p-CREB1/CREB1 ratio (p = 0.0309) while total CREB1 remained unchanged. qPCR confirmed a 2-fold increase in BDNF mRNA (p = 0.0495) without significant change in CREB transcripts. In conclusion, HBOT enhances hippocampal LTP in healthy rats by up-regulating the BDNF/p-CREB1 axis, revealing a novel neuromodulatory effect beyond injury models. These findings open avenues for its use in non-pathological contexts and warrant further mechanistic and translational studies.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"584 ","pages":"Pages 279-285"},"PeriodicalIF":2.8,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A narrative review on exosomes therapeutics in stroke: advancing neuroprotection and regeneration 外泌体治疗脑卒中的综述：促进神经保护和再生

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-25 DOI: 10.1016/j.neuroscience.2025.08.044

Muhammad Liaquat Raza , Masooma Fatima , Mohadisa Asif Rawalia , Ruqaiyya Raza

{"title":"A narrative review on exosomes therapeutics in stroke: advancing neuroprotection and regeneration","authors":"Muhammad Liaquat Raza , Masooma Fatima , Mohadisa Asif Rawalia , Ruqaiyya Raza","doi":"10.1016/j.neuroscience.2025.08.044","DOIUrl":"10.1016/j.neuroscience.2025.08.044","url":null,"abstract":"<div><div>The complexities of neuroinflammatory processes and the limited regenerative capacity of neural tissues have made ischemic stroke one of the leading causes of disability in many countries for the long periods after the initial incidence. Extracellular vesicles (EVs), more particularly exosomes, are emerging as promising agents for therapeutic usage, because of their outstanding ability to alter immune response and minimize oxidative stress suffered by neural tissue, and to enhance neuronal repair. The narrative review covers exosome biogenesis, particular cargo components, and mechanistic properties of exosomes in stroke therapy. It evaluates neuroprotection along with neurogenesis in both preclinical and clinical models, amassing their findings in immune modulation. Exosomes mediate neuroprotection through cargo-driven mechanisms involving miRNAs (e.g., miR-124, miR-21), stress-responsive proteins (e.g., HSP70), and bioactive lipids, facilitating neural repair post-ischemia. This review provides an in-depth analysis of exosome biogenesis, their cargo profiles, and the translational potential of exosome-based therapies in ischemic stroke. It discusses some of the advantages of exosome therapies over traditional cell-based therapies, such as greater safety profiles and more prolonged storage stability, based on the latest literature. Notwithstanding the challenges presented by exosome isolation and targeted delivery, their therapeutic possible applications deserve increasing attention. However, current translational efforts focus on optimizing exosome sourcing, improving targeting accuracy, and ensuring safety, positioning this innovative approach as a compelling candidate for next-generation stroke interventions with the potential to improve outcomes and facilitate personalized medicine.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"584 ","pages":"Pages 311-322"},"PeriodicalIF":2.8,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sleep modulation via Nucleus Accumbens A2AR neurons to probe cognition and behavior in mice 伏隔核A2AR神经元对小鼠认知和行为的睡眠调节

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-24 DOI: 10.1016/j.neuroscience.2025.08.015

Natasha W. Grabau , Vander W. LeKites , Emma K. Brousseau , Danielle L. Anzules , Eva M. Ortiz-Feder , Ramalingam Vetrivelan , William D. Todd , Daniel Kroeger

{"title":"Sleep modulation via Nucleus Accumbens A2AR neurons to probe cognition and behavior in mice","authors":"Natasha W. Grabau , Vander W. LeKites , Emma K. Brousseau , Danielle L. Anzules , Eva M. Ortiz-Feder , Ramalingam Vetrivelan , William D. Todd , Daniel Kroeger","doi":"10.1016/j.neuroscience.2025.08.015","DOIUrl":"10.1016/j.neuroscience.2025.08.015","url":null,"abstract":"<div><div>A key tool to investigate the putative function(s) of sleep is a model system in which sleep can be manipulated to design <em>gain-of-function</em> experiments and <em>loss-of-function</em> experiments i.e. increasing and decreasing sleep amounts to assess the effects on outcome variables. Here we utilize adenosine 2A receptor expressing neurons in the Nucleus Accumbens core (NAcc<sup>A2AR</sup> neurons) to perform gain-of-function experiments by controlling natural sleep circuits in mice to assess the effects of varying amounts of sleep on cognition and mood. Specifically, we either enhanced NREM sleep and REM sleep by chemogenetic activation of NAcc<sup>A2AR</sup> neurons in our mice, allowed them to sleep normally, or sleep deprived them before running them through the novel object recognition test, open field test, and the forced swim test to assess memory performance as well as levels of anxiety- and depression-like behaviors. Our results suggest a linear relationship between amounts of sleep and levels of anxiety-related behaviors, in that more sleep is positively correlated with higher levels of anxiety.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"584 ","pages":"Pages 334-344"},"PeriodicalIF":2.8,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expressive suppression moderates the relationship between brain volume and mental health 表达抑制调节脑容量与心理健康之间的关系。

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-23 DOI: 10.1016/j.neuroscience.2025.08.040

Maribel Delgado-Herrera , Brenda I. Arce , Victor E. Olalde-Mathieu , Giovanna L. Licea-Haquet , Azalea Reyes-Aguilar

{"title":"Expressive suppression moderates the relationship between brain volume and mental health","authors":"Maribel Delgado-Herrera , Brenda I. Arce , Victor E. Olalde-Mathieu , Giovanna L. Licea-Haquet , Azalea Reyes-Aguilar","doi":"10.1016/j.neuroscience.2025.08.040","DOIUrl":"10.1016/j.neuroscience.2025.08.040","url":null,"abstract":"<div><div>Emotion regulation plays a critical role in both physiological and psychological well-being. While previous structural neuroimaging studies have examined the relationship between emotion regulation strategies and brain volume in neurotypical individuals, the potential moderating role of these strategies in the association between brain volume and mental health variables remains largely unexplored. This study had three main objectives: (1) to employ an unsupervised, data-driven approach to cluster participants based on their use of cognitive reappraisal (CR) and expressive suppression (ES), alongside their scores on anxiety, depression, and perceived stress; (2) to compare the brain volume of regions of interest associated with emotion regulation across these groups; and (3) to investigate whether the frequent use of CR or ES moderates the relationship between brain volume and mental health outcomes. Fifty-three healthy adult participants completed self-report measures assessing their use of CR and ES, as well as anxiety, depression, and stress levels. High-resolution T1-weighted structural MRI scans were obtained to calculate brain volume. Unsupervised clustering identified two distinct groups based on emotion regulation strategy use and mental health scores. Cluster 1 showing greater volume in the left rectus gyrus compared to Cluster 2. Furthermore, moderation analyses revealed that frequent use of ES moderated the positive relationship between mental health symptoms and brain volume in the right superior frontal gyrus, left rectus gyrus, right amygdala, and bilateral insula. These findings underscore the importance of emotion regulation strategies in shaping mental health outcomes and their potential influence on brain structure in neurotypical individuals.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"585 ","pages":"Pages 60-70"},"PeriodicalIF":2.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dopamine stabilizer (-)-OSU6162 attenuates levodopa-induced dyskinesia in the unilateral 6-hydroxydopamine rat model of Parkinson’s disease 多巴胺稳定剂(-)- osu6162减轻帕金森病单侧6-羟多巴胺大鼠模型中左旋多巴诱导的运动障碍

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-23 DOI: 10.1016/j.neuroscience.2025.08.041

Daniela Atanasovski, Filip Bergquist, Elias Eriksson, Thomas Carlsson

{"title":"The dopamine stabilizer (-)-OSU6162 attenuates levodopa-induced dyskinesia in the unilateral 6-hydroxydopamine rat model of Parkinson’s disease","authors":"Daniela Atanasovski, Filip Bergquist, Elias Eriksson, Thomas Carlsson","doi":"10.1016/j.neuroscience.2025.08.041","DOIUrl":"10.1016/j.neuroscience.2025.08.041","url":null,"abstract":"<div><div>Levodopa-induced dyskinesia (LID) is a complication that occurs in many patients with Parkinson’s disease. (-)-OSU6162 is a clinically tolerable dopamine stabilizer with affinity for both dopaminergic D<sub>2</sub> receptors and serotonergic 5-HT<sub>2A</sub> receptors which has been shown to counteract LID in non-human primates. To investigate whether (-)-OSU6162 can dampen levodopa-induced abnormal involuntary movements in the unilateral 6-OHDA rat model of Parkinson’s disease without impairing levodopa-induced improvement in motor functioning, female Sprague Dawley rats received complete unilateral lesioning of the medial forebrain bundle and were treated daily with levodopa/benserazide. Animals that developed dyskinetic behavior as assessed by involuntary abnormal movements following two weeks of treatment with levodopa/benserazide received either (-)-OSU6162 (30 mg/kg) or saline immediately prior to their next levodopa/benserazide dose. In a separate batch of animals, the same add-on treatment was given following three days of levodopa/benserazide administration prior to subjecting the animals to the stepping and cylinder tests. (-)-OSU6162 was found to significantly reduce levodopa-induced abnormal involuntary movements in dyskinetic rats without compromising the effect of levodopa in the stepping test. In conclusion, add-on of (-)-OSU6162 attenuates the expression of LID in the 6-OHDA rat model of Parkinson’s disease without affecting the antiparkinsonian effect of levodopa.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"584 ","pages":"Pages 404-411"},"PeriodicalIF":2.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs in neuroplasticity: a comprehensive review of mechanisms and therapeutic strategies for neurodegenerative diseases 神经可塑性中的microrna：神经退行性疾病的机制和治疗策略的综合综述。

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-23 DOI: 10.1016/j.neuroscience.2025.08.034

Pedro César de Souza , Thaynara Paula Warren Bezerra , Isabella Luiza Ralph de Oliveira , Paulo André Teixeira de Morais Gomes , Moacyr Jesus Barreto de Melo Rêgo , Michelly Cristiny Pereira , Maira Galdino da Rocha Pitta , Michelle Melgarejo da Rosa

{"title":"MicroRNAs in neuroplasticity: a comprehensive review of mechanisms and therapeutic strategies for neurodegenerative diseases","authors":"Pedro César de Souza , Thaynara Paula Warren Bezerra , Isabella Luiza Ralph de Oliveira , Paulo André Teixeira de Morais Gomes , Moacyr Jesus Barreto de Melo Rêgo , Michelly Cristiny Pereira , Maira Galdino da Rocha Pitta , Michelle Melgarejo da Rosa","doi":"10.1016/j.neuroscience.2025.08.034","DOIUrl":"10.1016/j.neuroscience.2025.08.034","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) have emerged as key regulators of neuroplasticity, influencing essential processes such as neurogenesis, synaptogenesis, and neuroinflammatory responses. This review provides a comprehensive overview of the general roles of miRNAs in neuroplasticity and synthesizes recent insights from both preclinical and clinical studies, including transcriptomic analyses and miRNA profiling, to elucidate the molecular mechanisms by which these miRNAs modulate neuronal function. We have examined specific miRNAs, such as miR-132, miR-124, miR-134, miR-135 and miR-146a, and their roles in synaptic remodeling, neuronal differentiation, and regulation of neuroinflammation. Furthermore, we have explored the therapeutic potential of targeting these miRNAs in neurodegenerative diseases, with a focus on Alzheimer’s disease and Parkinson’s disease. The review highlights the novelty of miRNA-based interventions and innovative therapeutic delivery strategies, such as exosome-based systems, and lipid nanoparticles. Despite promising results in animal models, clinical translation remains limited due to challenges in delivery systems, off-target effects, and the need for validated biomarkers. Therefore, further studies, especially longitudinal trials in humans, are essential to advance the clinical utility of miRNA-targeted therapies in neurology.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"585 ","pages":"Pages 97-106"},"PeriodicalIF":2.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the influence of facial expressions on EEG-based biometric system performance in ADHD and healthy children 揭示面部表情对ADHD和健康儿童基于脑电图的生物识别系统性能的影响。

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-22 DOI: 10.1016/j.neuroscience.2025.08.042

Maryam Safardoost, Zahra Tabanfar, Farnaz Ghassemi

{"title":"Unveiling the influence of facial expressions on EEG-based biometric system performance in ADHD and healthy children","authors":"Maryam Safardoost, Zahra Tabanfar, Farnaz Ghassemi","doi":"10.1016/j.neuroscience.2025.08.042","DOIUrl":"10.1016/j.neuroscience.2025.08.042","url":null,"abstract":"<div><div>The growing need for reliable biometric systems to identify children in contexts such as vaccination tracking, missing child recovery, and hospital safety has highlighted the importance of using robust physiological markers. EEG signals have emerged as promising biometric indicators due to their resistance to external manipulation and forgery. In this study, we investigated the influence of emotional facial expressions on EEG-based biometric identification in children with and without Attention-Deficit/Hyperactivity Disorder (ADHD). EEG data were recorded from 25 typically developing (TD) children and 22 children diagnosed with ADHD during exposure to four emotional facial expressions: happy, sad, angry, and neutral. To evaluate the robustness of biometric identification across emotional states, brain connectivity features were extracted using various directed connectivity metrics (Directional Transfer Function (DTF), ffDTF, dDTF, dDTF08, Partial directional coherence (PDC)) and analyzed through clustering techniques based on Riemannian and Euclidean distances. The DTF feature combined with Riemannian distance-based clustering achieved the highest identification accuracies across all emotional states, reaching up to 100% for both groups. Specifically, accuracies of 99%, 99.4%, 99.6%, and 100% for healthy children and 100%, 99.77%, 99.77%, and 100% for ADHD children were obtained for sad, happy, angry, and neutral emotions, respectively. Statistical analysis confirmed the emotional resilience of the biometric system, showing no significant differences in identification accuracy between emotional states or between ADHD and TD groups. These findings support the feasibility of emotion-insensitive EEG-based biometric systems for child identification and highlight the utility of brain connectivity features in enhancing performance across neurodevelopmental populations.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"585 ","pages":"Pages 222-232"},"PeriodicalIF":2.8,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurotropism of alphaherpesviruses is most prominent in the mesiotemporal, piriform and prefrontal cortices in mice 甲疱疹病毒的嗜神经性在小鼠的中颞叶、梨状和前额叶皮层最为突出

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-22 DOI: 10.1016/j.neuroscience.2025.08.024

Viktoria Korff , Issam El-Debs , Barbara G. Klupp , Jens P. Teifke , Thomas C. Mettenleiter, Julia Sehl-Ewert

{"title":"Neurotropism of alphaherpesviruses is most prominent in the mesiotemporal, piriform and prefrontal cortices in mice","authors":"Viktoria Korff , Issam El-Debs , Barbara G. Klupp , Jens P. Teifke , Thomas C. Mettenleiter, Julia Sehl-Ewert","doi":"10.1016/j.neuroscience.2025.08.024","DOIUrl":"10.1016/j.neuroscience.2025.08.024","url":null,"abstract":"<div><div>Alphaherpesviruses, such as Herpes Simplex Virus 1 (HSV-1) and Pseudorabies Virus (PrV), exhibit pronounced neurotropism. HSV-1 can cause Herpes simplex encephalitis (HSE), primarily affecting the mesiotemporal lobe. CD1 mice inoculated with a PrV mutant (PrV-ΔUL21/US3Δkin) showed striking analogies to HSE, including comparable virus distribution and inflammatory patterns, providing a suitable model to analyze the specificity of the invasion route of alphaherpesviruses. Here, we investigated the strong preference for the mesiotemporal lobe by artificial targeted stereotactic inoculation of PrV-ΔUL21/US3Δkin and wildtype PrV-Kaplan into the temporal lobe or cerebellum in a kinetic approach. In the most severely affected brain areas viral antigen was quantified and correlated with the expression of the alphaherpesvirus entry receptor nectin-1. Temporal lobe inoculated mice showed viral antigen starting at 2dpi in this location. In contrast, only a low amount of viral antigen was found in the cerebellum after cerebellar inoculation but massive staining was observed in the mesiotemporal lobe at 7dpi. Correspondingly, cultured cerebral cortical neurons exhibited higher viral titers than cerebellar neurons, reflecting alphaherpesviral tropism for cerebral regions. Mice inoculated in the temporal lobe developed symptoms earlier than those inoculated into the cerebellum. PrV-Kaplan resulted in rapid lethality within 2 days, with antigen distribution mirroring that of PrV-ΔUL21/US3Δkin. High nectin-1 expression correlated strongly with viral antigen presence in the mesiotemporal lobe, piriform and prefrontal cortices. In summary, our studies demonstrate the high susceptibility of the mesiotemporal, piriform and prefrontal cortices upon artificial PrV inoculation, and highlight the suitability of this model for future investigations on HSE.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"584 ","pages":"Pages 367-381"},"PeriodicalIF":2.8,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A quantitative analysis of TAAR1-positive cells in whole mouse brain by FDISCO technology FDISCO技术定量分析小鼠全脑taar1阳性细胞

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-21 DOI: 10.1016/j.neuroscience.2025.08.025

De-Nong Liu , Meng Sun , Xian-Qiang Zhang, Ji-Tao Li, Tian-Mei Si, Yun-Ai Su

{"title":"A quantitative analysis of TAAR1-positive cells in whole mouse brain by FDISCO technology","authors":"De-Nong Liu , Meng Sun , Xian-Qiang Zhang, Ji-Tao Li, Tian-Mei Si, Yun-Ai Su","doi":"10.1016/j.neuroscience.2025.08.025","DOIUrl":"10.1016/j.neuroscience.2025.08.025","url":null,"abstract":"<div><div>Over the past two decades, studies on trace amine-associated receptor 1 (TAAR1), have substantially enhanced our understanding of their critical function in regulating monoamine neurotransmitter transmission. As a result, TAAR1 has emerged as a highly promising therapeutic target for treating psychiatric disorders. However, there is still no systematic analysis or detailed assessment for the distribution of TAAR1-positive cells throughout the brain until now. Herein, by applying CRISPR/Cas9-based approach and genetic labeling strategies, we constructed a TAAR1-Cre transgenic mouse and labeled TAAR1-positive cells with red fluorescence in whole brain. In combination with FDISCO technology, high-resolution imaging and automatic counting of stereoscopic cells, we conducted whole-brain three dimensions (3D) mapping, precise positioning and quantification of the TAAR1-positive cells. We found a diffuse distribution of TAAR1-positive cells throughout the brain. Higher number of TAAR1-positive cells were found in several brain regions including entorhinal area (ENT), sensory related area of medulla (MY-sen), Medial group of the dorsal thalamus (MED), basomedial amygdala (BMA), motor related area of medulla (MY-mot), and Cortical amygdala area (COA), all of which had more than 2,000. Moreover, the BMA and MED had the higher average density of TAAR1-positive cells compared to all of other subregions in the whole brain. In conclusion, this study unprecedentedly performed a systematic and exact whole-brain quantitative analysis of TAAR1-positive cells, providing a foundation for future investigations on the function of TAAR1 and TAAR1-positive cells, as well as delving deeper into the roles they play in the pathogenesis, development and treatment of multiple psychiatric disorders.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"584 ","pages":"Pages 286-294"},"PeriodicalIF":2.8,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of stress-induced hearing loss in repeated cold stress-exposed mice 反复冷应激小鼠应激性听力损失分析

IF 2.8 3区医学

Neuroscience Pub Date : 2025-08-20 DOI: 10.1016/j.neuroscience.2025.08.039

Takuya Sumi , Yoshihisa Koyama , Shoichi Shimada

{"title":"Analysis of stress-induced hearing loss in repeated cold stress-exposed mice","authors":"Takuya Sumi , Yoshihisa Koyama , Shoichi Shimada","doi":"10.1016/j.neuroscience.2025.08.039","DOIUrl":"10.1016/j.neuroscience.2025.08.039","url":null,"abstract":"<div><div>Sudden hearing loss is an acute sensorineural hearing loss of unknown etiology. Owing to the significant increase in the number of patients and low efficacy of treatment, elucidation of its pathogenesis is urgently required. An animal model for hearing loss is needed; however, mainstream models, such as noise exposure and drug administration, cause irreversible damage to the inner ear tissue and therefore cannot be said to reflect the pathology of sudden hearing loss, which can sometimes be cured spontaneously.</div><div>In this study, we prepared a mouse model of hearing loss caused by mental or physical stress due to a sudden change in temperature without directly damaging the inner ear. This mouse model exhibited a tendency toward increased physiological stress and a significant elevation in systemic inflammation. However, no evident structural abnormalities were observed in the inner ears of these mice. In contrast, significant neural activity was observed in the auditory cortices of these mice.</div><div>Our findings suggested that the onset of hearing loss in these mice may be related to increased systemic inflammation and prominent activity in the auditory cortex caused by excessive stress. Unlike previous mouse models of hearing loss, this stress-induced hearing loss mouse model is the first to be established in which hearing loss is induced by changes in the <em>in vivo</em> environment without causing significant damage to the inner ear tissue. A detailed analysis of this mouse model is expected to elucidate the causes and mechanisms of sudden hearing loss and establish treatment methods.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"584 ","pages":"Pages 269-278"},"PeriodicalIF":2.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0