Mycotoxin Research最新文献_第3页

Mycotoxins in brewing and malting: is every sample contaminated with mycotoxins? 酿酒和麦芽中的霉菌毒素：是否每个样品都被霉菌毒素污染？

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-12-30 DOI: 10.1007/s12550-024-00579-6

Marek Pernica, Jan Martiník, Rastislav Boško, Simona Černá, Zdeněk Svoboda, Karolína Benešová, Sylvie Běláková

{"title":"Mycotoxins in brewing and malting: is every sample contaminated with mycotoxins?","authors":"Marek Pernica, Jan Martiník, Rastislav Boško, Simona Černá, Zdeněk Svoboda, Karolína Benešová, Sylvie Běláková","doi":"10.1007/s12550-024-00579-6","DOIUrl":"10.1007/s12550-024-00579-6","url":null,"abstract":"Mycotoxins are secondary metabolites of fungi and represent a serious problem for human health. Due to growing interest, various aspects have been widely studied by scientific groups. One of these aspects relates to the food industry and associated beer production. Mycotoxins can be present in the basic raw materials for beer production as well as in brewed beer. Problematic mycotoxins that pose a serious risk of toxicity are aflatoxins especially aflatoxin B1 (AFB1), fumonisins (FBs), and zearalenone (ZEN) and its metabolites, deoxynivalenol (DON) including its acetylated forms and also the modified form deoxynivalenol-3-glucoside (DON-3G), T-2 toxin, HT-2 toxin, and ochratoxin A. The Research Institute of Brewing and Malting has been dealing with the issue of mycotoxins since 2008. This study describes the analysis of the above mycotoxins during 2020-2024 in barley (n = 775), malt (n = 751), and commercially available beers (n = 522) using QuEChERS, immunoaffinity columns, and UPLC-MS/MS. The results showed positive samples of mycotoxins in brewing and malting matrices at the level of micrograms per kilogram (barley, malt) and nanograms per liter for beer. Therefore, it is a residual concentration and the accurate quantitative determination of mycotoxins, correct interpretation of the results in connection with toxicological values, and the maximum permissible levels of mycotoxins play a key role in global food safety and consumer protection.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"239-248"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between aflatoxin M1 and immunoglobulin levels in cows' colostrum. 黄曲霉毒素 M1 与奶牛初乳中免疫球蛋白水平之间的关系。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-10-03 DOI: 10.1007/s12550-024-00565-y

Necdet Mutlu, Güler Yenice

{"title":"The relationship between aflatoxin M1 and immunoglobulin levels in cows' colostrum.","authors":"Necdet Mutlu, Güler Yenice","doi":"10.1007/s12550-024-00565-y","DOIUrl":"10.1007/s12550-024-00565-y","url":null,"abstract":"The purpose of the present research was to assess the amounts of aflatoxin M1 (AFM1) and immunoglobulin (IgA, IgG, and IgM) in cow colostrum samples, as well as their relationship. The sampling involved 90 cows (54 Montofon and 36 Simmental) from 15 independent farms. An appropriate number of samples from the total mixed ration (TMR) used in feeding the cows were collected simultaneously with the colostrum samples. AFB1 in feed, AFM1, and immunoglobulin (IgA, IgG, and IgM) levels in colostrum were evaluated using the ELISA method. The AFM1 level in colostrum samples exceeded the maximum allowed values for raw milk. Ig levels were higher in Montofon-breed cows' colostrum than in Simmentals. However, it was determined that neither the breed nor the lactation number significantly affected the colostrum Ig level. It was determined that there was no linear relationship between the number of lactations and immunoglobulin levels or between AFM1 and immunoglobulin levels in colostrum. Similarly, it was determined that there was no linear relationship between the AFB1 level in the feed and the AFM1 level in the colostrum of animals consuming these feeds.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"15-23"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pleurotus spp.-an effective way in degradation mycotoxins? A comprehensive review. Pleurotus spp.--降解霉菌毒素的有效方法？综述。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-11-12 DOI: 10.1007/s12550-024-00572-z

Agnieszka Zapaśnik, Marcin Bryła, Barbara Sokołowska, Agnieszka Waśkiewicz

{"title":"Pleurotus spp.-an effective way in degradation mycotoxins? A comprehensive review.","authors":"Agnieszka Zapaśnik, Marcin Bryła, Barbara Sokołowska, Agnieszka Waśkiewicz","doi":"10.1007/s12550-024-00572-z","DOIUrl":"10.1007/s12550-024-00572-z","url":null,"abstract":"Mycotoxins-secondary metabolites produced by filamentous fungal species-occur as a global problem in agriculture due to the reduction in crop quality and the negative effects on human and animal health. There is a need to develop environment-friendly methods of detoxification. In recent years, a number of biological methods for the removal/degradation of mycotoxins have been described. One of them-particularly interesting due to its high effectiveness-is mycoremediation, which involves the ability of Pleurotus spp. mushrooms to remove toxic contaminants from the environment and food. Pleurotus spp. biosynthesizes ligninolytic enzymes, such as laccase and manganese peroxidase that are the main factors of enzymatic degradation of various pollutants, including mycotoxins. The degradation process of mycotoxins (especially aflatoxins) with the participation of isolated enzymes reaches approximately 30-100%, depending on the culture conditions, substrate, and mediators used. In the food industry, their application may include, among others, the detoxification of animal feed from mycotoxins or fermentation products (e.g., juices and wines). While these applications are promising, they require further research to expand toxicological knowledge and optimize their use. This review presents current research on this new and very promising topic related to the use of edible Pleurotus spp. mushrooms in the process of biological degradation of toxic fungal metabolites.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"1-13"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycotoxin concentrations in rice are affected by chalkiness, grain shape, processing type, and grain origin. 大米中的霉菌毒素浓度受垩度、谷粒形状、加工类型和谷粒产地的影响。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-11-27 DOI: 10.1007/s12550-024-00575-w

Erasmus N Tang, Sali A Ndindeng, Geoffrey Onaga, Alejandro Ortega-Beltran, Titilayo D O Falade, Rousseau Djouaka, Michael Frei

{"title":"Mycotoxin concentrations in rice are affected by chalkiness, grain shape, processing type, and grain origin.","authors":"Erasmus N Tang, Sali A Ndindeng, Geoffrey Onaga, Alejandro Ortega-Beltran, Titilayo D O Falade, Rousseau Djouaka, Michael Frei","doi":"10.1007/s12550-024-00575-w","DOIUrl":"10.1007/s12550-024-00575-w","url":null,"abstract":"Mycotoxins such as aflatoxins (AFs), fumonisins (FBs), zearalenone (ZEN), and deoxynivalenol (DON) pose a risk to public health due to their carcinogenic potency (AFs and FBs) and anti-nutritional effects. The hazards associated with mycotoxins are accentuated where food management practices, control, and regulatory systems from farm to plate are sub-optimal. Information on the frequency of these mycotoxins in rice commercialized in markets in sub-Sahara Africa (SSA) is limited. The current study examined AF concentrations in 527 rice samples collected from 54 markets in five SSA countries. Grain quality characteristics, processing methods, and origin of samples were contrasted with toxin levels. In total, 72% of the samples had detectable AFs levels (range = 3.0 to 89.8 µg/kg). Forty-seven percent (47%) of the samples had AFs above 4 µg/kg, the European Union maximum level (ML), and were evaluated for cooccurrence with FBs, ZEN, and DON. Total AFs and ZEN cooccurred in 40% of the samples, and 30% of the positive ZEN samples had concentrations above the ML of 75 µg/kg. Total AFs did not co-occur with FBs and DON. Multivariate analysis revealed that length-to-width ratio (p < 0.0001), mixed variety for width (p = 0.04), and chalkiness (p = 0.009) significantly influenced aflatoxin concentrations. Slender grains had higher AFs concentrations than bold and medium grains (p < 0.0001). Possible strategies to mitigate mycotoxin contamination in rice include improving grain quality traits and practicing proper drying and hermetic storage before and after milling. These findings provide valuable insights for both domestic and international actors in establishing and strengthening regulations and management systems to mitigate rice mycotoxin contamination.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"163-177"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aflatoxin awareness and preventive agricultural practices are key to adoption of biocontrol among maize smallholder farmers in Tanzania. 对黄曲霉毒素的认识和预防性农业做法是坦桑尼亚玉米小农采用生物防治的关键。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-11-30 DOI: 10.1007/s12550-024-00574-x

Sambwe Fundikira, Rashid Suleiman, Sarah De Saeger, Marthe De Boevre, Martin Kimanya

{"title":"Aflatoxin awareness and preventive agricultural practices are key to adoption of biocontrol among maize smallholder farmers in Tanzania.","authors":"Sambwe Fundikira, Rashid Suleiman, Sarah De Saeger, Marthe De Boevre, Martin Kimanya","doi":"10.1007/s12550-024-00574-x","DOIUrl":"10.1007/s12550-024-00574-x","url":null,"abstract":"Aflatoxins are toxic secondary metabolites produced by Aspergillus species that infect staple foods like maize causing threat to public health and economic impacts. The use of atoxigenic Aspergillus species is considered one of the promising technologies to prevent aflatoxin contamination in maize. Tanzania approved the use of aflatoxin biocontrol (Aflasafe®) in 2018 and introduced it to eight districts. Adoption and effectiveness of this technology depend on many factors including application of pre- and post-harvest practices. There is scant information on awareness of biocontrol and factors which influence the adoption and effectiveness of this technology. A cross-sectional study was conducted in Tanzania to assess awareness and identify factors influencing adoption of the technology. Data was collected from 334 smallholder farmers in Kiteto and Chemba districts and analyzed using SPSS version 20; p-values < 0.05 using a two-tailed test were considered statistically significant. Results indicated 95.4% are not aware and that only 2.7% of the farmers had used biocontrol technology. The use of biocontrol was significantly associated with high income level (p = 0.001) and exposure to print media (p = 0.03) and radio (p = 0.008). The use of hybrid seed (p = 0.01), grazing (p = 0.017), and rotation of crops on yearly basis (p = 0.024) were also significantly associated with the use of biocontrol. Income limits the use of biocontrol, requiring government subsidies for Aflasafe and a premium market for aflatoxin-free maize. Aflatoxin awareness and sensitization on adherence to good pre-harvest practices should be emphasized to enhance adoption of the technology.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"179-189"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring Fusarium toxins from barley to malt: Targeted inoculation with Fusarium culmorum. 监测从大麦到麦芽的镰刀菌毒素：有针对性地接种镰刀菌。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-12-20 DOI: 10.1007/s12550-024-00573-y

Eva Maria Biehl, Sarah Schneidemann-Bostelmann, Felix Hoheneder, Stefan Asam, Ralph Hückelhoven, Michael Rychlik

{"title":"Monitoring Fusarium toxins from barley to malt: Targeted inoculation with Fusarium culmorum.","authors":"Eva Maria Biehl, Sarah Schneidemann-Bostelmann, Felix Hoheneder, Stefan Asam, Ralph Hückelhoven, Michael Rychlik","doi":"10.1007/s12550-024-00573-y","DOIUrl":"10.1007/s12550-024-00573-y","url":null,"abstract":"Molds of the genus Fusarium infect nearly all types of grain, causing significant yield and quality losses. Many species of this genus produce mycotoxins, which pose significant risks to human and animal health. In beer production, the complex interaction between primary fungal metabolites and secondarily modified mycotoxins in barley, malt, and beer complicates the situation, highlighting the need for effective analytical methods to quickly and accurately monitor these toxins. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to simultaneously analyze 14 Fusarium toxins, including modified forms (deoxynivalenol (DON), DON-3-glucoside, 3-acetyl-DON, 15-acetyl-DON, nivalenol, fusarenone X, HT-2 toxin, T-2 toxin, the enniatins A, A1, B, B1, beauvericin, and zearalenone) in barley and throughout the malting process. Stable isotope dilution assays (SIDAs) and matrix-matched calibration were used for quantification. A micro-malting setup was established to produce Fusarium-contaminated barley malt under reproducible conditions using targeted inoculation with F. culmorum. Mycotoxins were quantified throughout the malting process and compared to the content of fungal DNA. Further, the impact of various malting parameters was investigated, thus revealing that different malting scenarios exhibited different toxin enrichment patterns. We demonstrated that mycotoxin concentration and the ratio of DON to DON-3-glucoside changed throughout the malting processes, depending on fungal spore concentrations, germination temperature, and malting temperature. The study highlights the complexity of mycotoxin dynamics in malt production and the importance of optimized processing conditions to minimize toxin levels in final malt products.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"215-237"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of mycotoxins in milk thistle: to be included in legislation or not? 水飞蓟中真菌毒素的发生：应不应纳入立法？

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-12-10 DOI: 10.1007/s12550-024-00577-8

Rastislav Boško, Helena Pluháčková, Jan Martiník, Karolína Benešová, Zdeněk Svoboda, Sylvie Běláková, Marek Pernica

引用次数: 0

Assessment of mycotoxins in infant flour and their decontamination in raw material during production processes in Ouagadougou. 瓦加杜古婴儿面粉中真菌毒素的评估及其在生产过程中对原料的去污。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-12-05 DOI: 10.1007/s12550-024-00578-7

Léa K A Bayala-Yaї, Philippe A Nikièma, Bazoin S R Bazié, Fulbert Nikièma, Jacques Simpore

{"title":"Assessment of mycotoxins in infant flour and their decontamination in raw material during production processes in Ouagadougou.","authors":"Léa K A Bayala-Yaї, Philippe A Nikièma, Bazoin S R Bazié, Fulbert Nikièma, Jacques Simpore","doi":"10.1007/s12550-024-00578-7","DOIUrl":"10.1007/s12550-024-00578-7","url":null,"abstract":"The infant flours produced in Burkina Faso are essentially a mixture of cereals and legumes. These raw materials are frequently contaminated with mycotoxins which pose a huge food safety and public health threat. The objective of this study was to determine mycotoxin levels in raw materials and infant flours in Ouagadougou and to investigate the impact of decontamination on the raw materials used in infant flour production. A total of 22 cereals and 17 legumes as raw materials and 26 infant flour samples were analysed for aflatoxins, fumonisin B1 (FB1), and ochratoxin A (OTA) by liquid chromatography coupled to tandem mass spectrometry, while saline treatment and hand-sorting of grains in mycotoxin reduction were tested. All the samples of raw materials and infant flours were contaminated with aflatoxins, whereas 20.5% and 38.5% of raw materials and 57.7% and 61.5% of infant flours, respectively, were contaminated by FB1 and OTA. These decontamination assays significantly reduced the levels of mycotoxins. AFB1 was reduced by 48% after soaking of maize for 6 h in a 6% NaCl solution. Sorting resulted in a 92% reduction in AFB1 content in peanut. However, soaking in saline solution did not reduce the FB1 and OTA contents. Sorting did not also reduce FB1 contents in peanut. Sorting and soaking in 6% saline solution for 6 h are production processes that lead to a reduction in the level of contamination by aflatoxins in maize and peanut used as raw materials for infant flour production.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"191-198"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary exposure of school children to aflatoxin and fumonisin through githeri and effectiveness of nixtamalization in reduction of these toxins in githeri from Turkana County. 图尔卡纳县的学龄儿童通过肉糜摄入黄曲霉毒素和伏马菌素的情况，以及尼氏腌制法在减少肉糜中黄曲霉毒素和伏马菌素含量方面的效果。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-10-11 DOI: 10.1007/s12550-024-00570-1

Charles Mannara, Lucy Gicuku Njue, George Ooko Abong'

{"title":"Dietary exposure of school children to aflatoxin and fumonisin through githeri and effectiveness of nixtamalization in reduction of these toxins in githeri from Turkana County.","authors":"Charles Mannara, Lucy Gicuku Njue, George Ooko Abong'","doi":"10.1007/s12550-024-00570-1","DOIUrl":"10.1007/s12550-024-00570-1","url":null,"abstract":"Exposure of school children to aflatoxin and fumonisin is mainly through diet. In Kenyan public schools, children are given porridge made from maize flour for breakfast, a mixture of maize and beans, also known as githeri for lunch and ugali for dinner. Nixtamalization has proved to reduce mycotoxins in most cereals and not a mixture of maize and beans. This study, therefore, aimed to assess the exposure of primary school children in Turkana County to aflatoxin and fumonisin through maize-based food under the school meals program and the effectiveness of nixtamalization in the reduction of these mycotoxins. Samples of githeri were randomly collected from all public primary schools (n = 128) under the homegrown school meals program in Turkana County and analyzed for aflatoxin and fumonisin. The data was analyzed using SAS software, version 9.4. The deterministic model was used to calculate the estimated daily intake (EDI) and the margin of exposure (MOE) used to characterize the exposure risk. The contaminated samples were then treated with various concentrations of Ca(OH)2, 0.5-2.5%. The treated samples were cooked for 60 and 75 min and soaked for 6 and 8 h. Forty percent of the schools contained githeri samples with aflatoxin B1 levels above 5 µg/Kg, the maximum limit for Kenya. Exposure to aflatoxin B1 and total aflatoxin was up to 2 µg/kg/bw/day. The range for fumonisin exposure was 60-80 µg/kg/bw/day. Ca(OH)2 concentration levels of up to 2.5% reduced aflatoxin by 75% and fumonisin by 72%. The findings indicate that githeri is contaminated with aflatoxin and fumonisin which exposes school children to these mycotoxins and nixtamalization can be used to reduce mycotoxin contamination in githeri.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"37-45"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering Aflatoxin B1 affected critical molecular pathways governing cancer: A bioinformatics study using CTD and PANTHER databases. 解密黄曲霉毒素 B1 对癌症关键分子通路的影响：利用 CTD 和 PANTHER 数据库进行的生物信息学研究。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2025-02-01 Epub Date: 2024-10-17 DOI: 10.1007/s12550-024-00563-0

Ankita Kapri, Dheer Singh, Suneel Kumar Onteru

{"title":"Deciphering Aflatoxin B1 affected critical molecular pathways governing cancer: A bioinformatics study using CTD and PANTHER databases.","authors":"Ankita Kapri, Dheer Singh, Suneel Kumar Onteru","doi":"10.1007/s12550-024-00563-0","DOIUrl":"10.1007/s12550-024-00563-0","url":null,"abstract":"Aflatoxin B1 (AFB1) is a fungal toxin consistently found as a contaminant in food products such as cereals, nuts, spices, and oilseeds. AFB1 exposure can lead to hepatotoxicity, cancer, immune suppression, reproductive deficiency, nutritional dysfunction, and growth impairment. AFB1 has also been listed as one of the most potent human carcinogens by the International Agency for Research on Cancer. Although the correlation between AFB1 exposure and cancer initiation and progression is already reported in the literature, very little information is available about what molecular pathways are affected during cancer development. Considering this, we first selected AFB1-responsive genes involved in five deadliest cancer types including lung, colorectal, liver, stomach, and breast cancers from the Comparative Toxicogenomics Database (CTD). Then, using the PANTHER database, a statistical overrepresentation test was performed to identify the significantly affected pathways in each cancer type. The gonadotropin-releasing hormone receptor (GnRHR) pathway, the CCKR signaling pathway, and angiogenesis were found to be the most affected pathways in lung, breast, liver, and stomach cancers. In addition, AFB1 toxicity majorly impacted apoptosis and Wnt signaling pathways in liver and stomach cancers, respectively. Moreover, the most affected pathways in colorectal cancer were the Wnt, CCKR, and GnRHR pathways. Furthermore, gene analysis was also performed for the most affected pathways associated with each cancer and identified thirteen key genes (e.g., FOS, AKT1) that may serve as biological markers for a particular type of AFB1-induced cancer as well as for in vitro AFB1 toxicological studies using specific cancer cell lines.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"93-111"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0