Mycotoxin Research最新文献_第4页

Development and validation of extraction and clean-up procedures for UPLC-MS/MS analysis of aflatoxins in spices. 开发和验证用于 UPLC-MS/MS 分析香料中黄曲霉毒素的提取和净化程序。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-07-11 DOI: 10.1007/s12550-024-00546-1

Ranjith Arimboor, Venugopal Gopalan, Srilatha C M, Remashree Azhimala Bhaskaranpillai

{"title":"Development and validation of extraction and clean-up procedures for UPLC-MS/MS analysis of aflatoxins in spices.","authors":"Ranjith Arimboor, Venugopal Gopalan, Srilatha C M, Remashree Azhimala Bhaskaranpillai","doi":"10.1007/s12550-024-00546-1","DOIUrl":"10.1007/s12550-024-00546-1","url":null,"abstract":"UPLC-MS/MS analytical conditions for the analysis of aflatoxins in spices were optimized and validated in this study. Liquid-liquid partition-based protocols for the cleaning up of extracts using common organic solvents such as acetonitrile, hexane, and ethyl acetate were developed and validated. The developed liquid-liquid partition methods were compared with immuno-affinity column and QuEChERS clean-up methods for the UPLC-MS/MS analysis of aflatoxins in 8 spices. The reduction of lipophilic components using the partition with hexane is particularly useful in spices like red pepper that have higher levels of fatty acids, carotenoids, sterols, triterpenoids, etc. The subsequent partitioning with ethyl acetate considerably reduced the matrix interference from the polar components and increased the sensitivity. The cleaning up of spice extracts using liquid-liquid partition techniques resulted in limits of quantification (LOQ) of 2-5 µgL-1 in UPLC-MS/MS analysis. Trueness, repeatability, and reproducibility of the methods were in acceptable ranges. The accuracy of the developed methods was further verified by analyzing aflatoxins in naturally incurred samples of spices and comparing the results with those obtained from the immuno-affinity column cleanup-HPLC-FD method.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"559-568"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141580351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycotoxigenic Fusarium species and zearalenone concentration in commercial maize kernels in northern Ghana. 加纳北部商品玉米粒中的霉菌毒素镰刀菌种和玉米赤霉烯酮浓度。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-07-18 DOI: 10.1007/s12550-024-00544-3

Nelson Opoku, Abdul Rashid Hudu, Francis Addy

{"title":"Mycotoxigenic Fusarium species and zearalenone concentration in commercial maize kernels in northern Ghana.","authors":"Nelson Opoku, Abdul Rashid Hudu, Francis Addy","doi":"10.1007/s12550-024-00544-3","DOIUrl":"10.1007/s12550-024-00544-3","url":null,"abstract":"The fungal genus Fusarium contains many toxigenic pathogens of maize with associated yield losses, reduction of grain quality, and accumulation of mycotoxins in harvested grains. To determine zearalenone (ZEN) concentration and identify the various Fusarium species in commercial maize grains, a survey of 75 maize samples, collected from 11 market centers in the five regions in northern Ghana was identified based on morphological characteristics, sequence analysis of the internal transcribed spacer region, and polymerase chain reaction using species-specific primers. ZEN levels were determined using HPLC. ZEN contamination was recorded in 33.3% of the maize samples, with concentrations ranging from 0.61 to 3.05 µg/kg. Based on VERT1/2 and TEF 1-α sequencing, F. verticillioides was the most prevalent species in the studied samples: 40.35% from the Upper East Region, 28.07% from the North East Region, 19.30% from the Upper West Region, 10.53% from the Savannah Region, and 1.75% for the Northern Region. Other fungal species found were F. equiseti and F. solani. A higher number of the Fusarium isolates were found in white maize (609 isolates from 27 samples) compared to yellow maize (225 isolates from 23 samples).","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"581-590"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141634012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of different abiotic conditions on the concentrations of free and conjugated deoxynivalenol and zearalenone in stored wheat. 不同非生物条件对储藏小麦中游离和共轭脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮浓度的影响。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-07-19 DOI: 10.1007/s12550-024-00541-6

Abimbola Oluwakayode, Brett Greer, Qiqi He, Michael Sulyok, Julie Meneely, Rudolf Krska, Angel Medina

{"title":"The influence of different abiotic conditions on the concentrations of free and conjugated deoxynivalenol and zearalenone in stored wheat.","authors":"Abimbola Oluwakayode, Brett Greer, Qiqi He, Michael Sulyok, Julie Meneely, Rudolf Krska, Angel Medina","doi":"10.1007/s12550-024-00541-6","DOIUrl":"10.1007/s12550-024-00541-6","url":null,"abstract":"Environmental factors influence fungal growth and mycotoxin production in stored grains. However, the concentrations of free mycotoxins and their conjugates and how they are impacted by different interacting environment conditions have not been previously examined. The objectives of this study were to examine the impact of storage conditions (0.93-0.98 aw) and temperature (20-25 °C) on (a) the concentrations of deoxynivalenol and zearalenone and their respective glucosides/conjugates and (b) the concentrations of emerging mycotoxins in both naturally contaminated and irradiated wheat grains inoculated with Fusarium graminearum. Contaminated samples were analysed for multiple mycotoxins using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Method validation was performed according to the acceptable performance criteria set and updated by the European Commission regulations No. 2021/808/EC. As an important conjugate of deoxynivalenol, the concentrations of deoxynivalenol-3-glucoside were significantly different from its precursor deoxynivalenol at 0.93 aw (22% moisture content- MC) at 25 °C in the naturally contaminated wheat with a ratio proportion of 56:44% respectively. The high concentrations of deoxynivalenol-3-glucoside could be influenced by the wheat's variety and/or harvested season/fungal strain type/location. Zeralenone-14-sulfate concentrations were surprisingly three times higher than Zearalenone in the naturally contaminated wheat at 0.98 aw (26% MC) at both temperatures. Emerging mycotoxins such as moniliformin increased with temperature rise with the highest concentrations at 0.95 aw and 25 °C. These findings highlight the influence and importance of storage aw x temperature conditions on the relative presence of free vs conjugated mycotoxins which can have implications for food safety.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"591-603"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11480129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141723974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a rapid method for determination of Ochratoxin A in grape mash and wine. 开发测定葡萄泥和葡萄酒中赭曲霉毒素 A 的快速方法。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-07-21 DOI: 10.1007/s12550-024-00543-4

Efanova Yulia, Pour Nikfardjam Martin

引用次数: 0

Factors influencing fumonisin B₁ contamination in maize: insights from two production regions in Costa Rica. 影响玉米中伏马菌素 B1 污染的因素：哥斯达黎加两个产区的启示。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-08-17 DOI: 10.1007/s12550-024-00551-4

Katherine Sánchez-Zúñiga, William Rivera-Méndez, Stefany Campos-Boza, Emanuel Araya-Valverde, Frank Solano-Campos, Maria Vinas

{"title":"Factors influencing fumonisin B1 contamination in maize: insights from two production regions in Costa Rica.","authors":"Katherine Sánchez-Zúñiga, William Rivera-Méndez, Stefany Campos-Boza, Emanuel Araya-Valverde, Frank Solano-Campos, Maria Vinas","doi":"10.1007/s12550-024-00551-4","DOIUrl":"10.1007/s12550-024-00551-4","url":null,"abstract":"Maize (Zea mays L.) is an important cereal crop worldwide. Contaminated maize kernels pose a significant mycotoxin exposure risk for humans in Latin America. Fumonisins, the most prevalent mycotoxin in maize, typically occur during pre-harvest conditions leading to significant economic losses. Various factors, including weather conditions, may influence this contamination. This study aimed to determine the association between fumonisin B1 (FB1) contamination, prevalence of Fusarium verticillioides, weather conditions and kernel quality in the two primary maize production areas in Costa Rica (Brunca and Chorotega). All maize samples (100%) showed FB1 contamination, with higher concentrations in samples from Brunca region, consistent with the presence of F. verticilliodes. Weather conditions appeared to play an important role in this contamination, since Brunca region had the highest mean temperature and relative humidity after maize silking (R1) and the total monthly rainfall in this region was significantly higher during the last two months of maize cultivation (grain-filling and physiological maturity stages R3 to R6). Interestingly, this study found a negative correlation between grain damage and kernel contamination with FB1 and F. verticillioides. The concentration of mineral nutrients in kernels from both regions was largely similar. Most nutrients in kernels exhibited a negative correlation with FB1, particularly nitrogen. Zinc and phosphorus were the only nutrients in kernels showing a positive correlation with FB1 in samples from the Brunca region. The results highlight elevated levels of FB1 contamination in maize and contribute to a better understanding of pre-harvest factors influencing FB1 contamination in tropical conditions.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"641-649"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141996178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ochratoxin a levels in Turkish coffee: a probabilistic health risk assessment via Monte Carlo simulation. 土耳其咖啡中的赭曲霉毒素 a 含量：通过蒙特卡罗模拟进行概率健康风险评估。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-08-20 DOI: 10.1007/s12550-024-00552-3

Sumeyra Sevim, Arife Macit, Mevlude Kizil

{"title":"Ochratoxin a levels in Turkish coffee: a probabilistic health risk assessment via Monte Carlo simulation.","authors":"Sumeyra Sevim, Arife Macit, Mevlude Kizil","doi":"10.1007/s12550-024-00552-3","DOIUrl":"10.1007/s12550-024-00552-3","url":null,"abstract":"Throughout history, Turkish coffee has been the most widely consumed type of coffee in Turkey. Therefore, the goal of this study was to determine the amount of ochratoxin A (OTA) present in Turkish coffee and to analyze any potential health hazards. A total of 41 Turkish coffees were collected and analyzed for OTA activity using a competitive enzyme immunoassay (ELISA). Furthermore, dietary exposure and health risk assessments for the Turkish population were calculated based on analytical results and coffee consumption data from the Turkish Nutrition and Health Survey 2019 (TNHS-2019) in three age categories (15-18, 19-64, and 65 + years). Nine of the samples contained more than 2.5 μg/kg OTA, with an average of 5.24 μg/kg. The OTA concentration in 3 samples exceeded the permissible maximum limit (5 μg/kg) established by Turkish legislation, and the mean concentration was 8.41 μg/kg. A margin of exposure (MOE) approach was used for risk characterization, considering both non-neoplastic and neoplastic consequences. There were no concerns about health risks because MOEs were more than 10,000 for all categories. Although the levels of OTA analyzed in Turkish coffee did not pose a risk to individuals in the three age categories, emphasis should be placed on minimizing and controlling OTA concentrations in Turkish coffee. Additionally, it is also necessary to consider other food sources that could contribute to OTA exposure.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"651-658"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on inhibitors and inhibitory mechanisms of mycotoxin biosynthesis. 霉菌毒素生物合成抑制剂和抑制机制的研究进展。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-08-21 DOI: 10.1007/s12550-024-00553-2

Mengjie Li, Honghua Li

引用次数: 0

Identification of mycotoxins in yogurt samples using an optimized QuEChERS extraction and UHPLC-MS/MS detection. 利用优化的 QuEChERS 萃取和超高效液相色谱-质谱/质谱检测技术鉴定酸奶样品中的霉菌毒素。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-07-17 DOI: 10.1007/s12550-024-00547-0

Inés Rodríguez-Cañás, Jesús M González-Jartín, Rebeca Alvariño, Amparo Alfonso, Mercedes R Vieytes, Luis M Botana

{"title":"Identification of mycotoxins in yogurt samples using an optimized QuEChERS extraction and UHPLC-MS/MS detection.","authors":"Inés Rodríguez-Cañás, Jesús M González-Jartín, Rebeca Alvariño, Amparo Alfonso, Mercedes R Vieytes, Luis M Botana","doi":"10.1007/s12550-024-00547-0","DOIUrl":"10.1007/s12550-024-00547-0","url":null,"abstract":"Yogurt, a milk-derived product, is susceptible to mycotoxin contamination. While various methods have been developed for the analysis of dairy products, only a few have been specifically validated for yogurt. In addition, these methods are primarily focus on detecting aflatoxins and zearalenone. This study aimed to conduct a preliminary investigation into the presence of regulated, emerging, and modified mycotoxins in natural and oat yogurts available in the Spanish market. For this, a QuEChERS-based extraction method was optimized and then validated to detect and quantify 32 mycotoxins using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The method was in-house validated for the analysis of natural and oat yogurt in terms of linearity, matrix effect, sensitivity, accuracy, and precision. Satisfactory performance characteristics were achieved; for most of the analytes, LOQs were lower than 2 ng/g, and recoveries ranged from 60 to 110% with a precision, expressed as the relative standard deviation of the recovery, lower than 15%. Subsequently, the validated method was applied to analyze commercial yogurt samples, revealing a notable incidence of beauvericin and enniatins, with some analogues found in up to 100% of the samples. Alternariol methyl ether was also frequently found, appearing in 50% of the samples. Additionally, the study identified regulated toxins such as fumonisins, ochratoxin A , and HT-2 toxin. These results provide new incidence data in yogurt, raising concerns about potential health risks for consumers.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"569-579"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aflatoxin B1-associated oxidative stress along with toxicopathological and immunological alterations is efficiently counteracted by dietary supplementation of distillery yeast sludge in broilers. 在肉鸡日粮中添加酒厂酵母污泥可有效抵消黄曲霉毒素 B1 导致的氧化应激以及毒性病理和免疫学改变。

IF 2.6 4区医学

Mycotoxin Research Pub Date : 2024-11-01 Epub Date: 2024-07-29 DOI: 10.1007/s12550-024-00549-y

Aisha Khatoon, Muhammad Zargham Khan, Zain Ul Abidin, Muhammad Kashif Saleemi, Halis Oguz, Shafia Tehseen Gul, Rao Zahid Abbas, Ashiq Ali, Sheraz Ahmad Bhatti

{"title":"Aflatoxin B1-associated oxidative stress along with toxicopathological and immunological alterations is efficiently counteracted by dietary supplementation of distillery yeast sludge in broilers.","authors":"Aisha Khatoon, Muhammad Zargham Khan, Zain Ul Abidin, Muhammad Kashif Saleemi, Halis Oguz, Shafia Tehseen Gul, Rao Zahid Abbas, Ashiq Ali, Sheraz Ahmad Bhatti","doi":"10.1007/s12550-024-00549-y","DOIUrl":"10.1007/s12550-024-00549-y","url":null,"abstract":"Aflatoxin B1 (AFB1) is among the most potent genotoxic and carcinogenic mycotoxins and is a major source of distress for the growing poultry sector. On the other hand, distillery yeast sludge or distillery sludge (DS) is a byproduct of molasses-based industries. It is often treated as a waste despite containing abundant nutrients particularly protein, basic amino acids, and vitamins along with other macro and micronutrients. This study was designed to investigate the oxidative stress and immunological alterations induced by AFB1 and their amelioration by dietary supplementation with DS. For this purpose, 360 newly hatched broiler chicks were randomly divided into twelve groups (30 birds each) and fed different combinations of AFB1 (100, 200, or 600 µg/kg) and DS (5 or 10 g/kg) for 42 days. The parameters under consideration were body weight, feed conversion ratio (FCR), relative organ weights, histopathological examination of different visceral organs, total antioxidant capacity, antibody response to intravenous injection of sheep red blood cells, in situ lymphoproliferative response to phytohemagglutinin-P, and phagocytic potential through a carbon clearance assay system. The results of this study established that DS supplementation ameliorated AFB1-associated oxidative stress and ameliorated toxicopathological and immunological anomalies in groups given AFB1 at 100 µg/kg and 200 µg/kg; however, little to no relief was observed in birds fed AFB1 at 600 µg/kg. The determination of the actual ratio of the AFB1 to the DS for substantiating the ameliorating effects requires further investigation.","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":" ","pages":"615-629"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactions of citrinin with amino compounds modelling thermal food processing 食品热加工模型中柠檬素与氨基化合物的反应

IF 3 4区医学

Mycotoxin Research Pub Date : 2024-09-19 DOI: 10.1007/s12550-024-00557-y

Lea Brückner, Benedikt Cramer, Hans-Ulrich Humpf

引用次数: 0