Mycotoxin Research最新文献

{"title":"The role of chromatin-modifying enzymes and histone modifications in the modulation of p16 gene in fumonisin B₁-induced toxicity in human kidney cells.","authors":"Ecem Fatma Karaman,&nbsp;Mahmoud Abudayyak,&nbsp;Sibel Ozden","doi":"10.1007/s12550-023-00494-2","DOIUrl":"https://doi.org/10.1007/s12550-023-00494-2","url":null,"abstract":"<p><p>Fumonisin B₁ (FB₁) poses a risk to animal and human health. Although the effects of FB₁ on sphingolipid metabolism are well documented, there are limited studies covering the epigenetic modifications and early molecular alterations associated with carcinogenesis pathways caused by FB₁ nephrotoxicity. The present study investigates the effects of FB₁ on global DNA methylation, chromatin-modifying enzymes, and histone modification levels of the p16 gene in human kidney cells (HK-2) after 24 h exposure. An increase (2.23-fold) in the levels of 5-methylcytosine (5-mC) at 100 µmol/L was observed, a change independent from the decrease in gene expression levels of DNA methyltransferase 1 (DNMT1) at 50 and 100 µmol/L; however, DNMT3a and DNMT3b were significantly upregulated at 100 µmol/L of FB₁. Dose-dependent downregulation of chromatin-modifying genes was observed after FB₁ exposure. In addition, chromatin immunoprecipitation results showed that 10 µmol/L of FB₁ induced a significant decrease in H3K9ac, H3K9me3 and H3K27me3 modifications of p16, while 100 µmol/L of FB₁ caused a significant increase in H3K27me3 levels of p16. Taken together, the results suggest that epigenetic mechanisms might play a role in FB₁ carcinogenesis through DNA methylation, and histone and chromatin modifications.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 3","pages":"271-283"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9933714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparing the effects of essential oils and methanolic extracts on the inhibition of Aspergillus flavus and Aspergillus parasiticus growth and production of aflatoxins.","authors":"Lilian D Kaale","doi":"10.1007/s12550-023-00490-6","DOIUrl":"https://doi.org/10.1007/s12550-023-00490-6","url":null,"abstract":"<p><p>The antifungal and antiaflatoxigenic effects of four distinct plant species against Aspergillus flavus and Aspergillus parasiticus were investigated. Essential oils and methanolic extracts were prepared from aerial parts of Lippia javanica, Ocimum gratissimum, Satureja punctata, and stem barks of Toddalia asiatica by hydro-distillation and maceration, respectively. The poisoned food method was used to confirm the antifungal activity of essential oils and methanolic extracts from four different plant species against Aspergillus flavus and Aspergillus parasiticus, and high-performance liquid chromatography was used to quantify the antiaflatoxigenic activity. The essential oils of Satureja punctata and Lippia javanica showed the highest antiaflatoxigenic activity against the fungi strains tested at concentrations of 1.25, 2.5, and 5 µL/mL, followed by Ocimum gratissimum essential oil while Toddalia asiatica essential oil exerted moderate antiaflatoxigenic activity. Meanwhile, the methanolic extracts showed a wide spectrum of low to high antifungal and antiaflatoxigenic activities at concentrations of 125, 250, and 500 µg/mL against A. flavus and A. parasiticus. This study has indicated that the essential oils of Satureja punctate, Lippia javanica, and Ocimum gratissimum had substantial antifungal and antiaflatoxigenic activities compared to their methanolic extracts, while Toddalia asiatica methanolic extract had a moderate antifungal activity compared to its essential oil.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 3","pages":"233-245"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9917077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycotoxigenic fungal growth inhibition and multi-mycotoxin reduction of potential biological control agents indigenous to grain maize.","authors":"Siti Nur Ezzati Yazid,&nbsp;Nur Izzah Tajudin,&nbsp;Nur Aina Aribah Razman,&nbsp;Jinap Selamat,&nbsp;Siti Izera Ismail,&nbsp;Maimunah Sanny,&nbsp;Nik Iskandar Putra Samsudin","doi":"10.1007/s12550-023-00484-4","DOIUrl":"https://doi.org/10.1007/s12550-023-00484-4","url":null,"abstract":"<p><p>The present work investigated the potential of fungal species from grain maize farms in Malaysia as antagonists against the indigenous mycotoxigenic fungal species and their subsequent mycotoxin production. Dual-culture assay was conducted on grain maize agar (GMA) with 12 strains of potential fungal antagonists namely Bjerkandra adusta, Penicillium janthinellum, Schizophyllum commune, Trametes cubensis, Trichoderma asperelloides, Trichoderma asperellum, Trichoderma harzianum, and Trichoderma yunnanense against seven mycotoxigenic strains namely Aspergillus flavus, Aspergillus niger, Fusarium verticillioides, and Fusarium proliferatum producing aflatoxins, ochratoxin A, and fumonisins, respectively. Based on fungal growth inhibition, Trichoderma spp. showed the highest inhibitory activity (73-100% PIRG, Percentage Inhibition of Radial Growth; 28/0 I_D, Index of Dominance) against the tested mycotoxigenic strains. Besides, B. adusta and Tra. cubensis showed inhibitory activity against some of the tested mycotoxigenic strains. All fungal antagonists showed varying degrees of mycotoxin reduction. Aflatoxin B₁ produced by A. flavus was mainly reduced by P. janthinellum, Tra. cubensis, and B. adusta to 0 ng/g. Ochratoxin A produced by A. niger was mainly reduced by Tri. harzianum and Tri. asperellum to 0 ng/g. Fumonisin B₁ and FB₂ produced by F. verticillioides was mainly reduced by Tri. harzianum, Tri. asperelloides, and Tri. asperellum to 59.4 and 0 µg/g, respectively. Fumonisin B₁ and FB₂ produced by F. proliferatum were mainly reduced by Tri. asperelloides and Tri. harzianum to 244.2 and 0 µg/g, respectively. This is the first study that reports on the efficacy of Tri. asperelloides against FB₁, FB₂, and OTA, P. janthinellum against AFB₁, and Tra. cubensis against AFB₁.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 3","pages":"177-192"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10292639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deoxynivalenol affects cell metabolism in vivo and inhibits protein synthesis in IPEC-1 cells.","authors":"Constanze Nossol,&nbsp;Peter Landgraf,&nbsp;Anikó Barta-Böszörmenyi,&nbsp;Stefan Kahlert,&nbsp;Jeannette Kluess,&nbsp;Berend Isermann,&nbsp;Oliver Stork,&nbsp;Daniela C Dieterich,&nbsp;Sven Dänicke,&nbsp;H-J Rothkötter","doi":"10.1007/s12550-023-00489-z","DOIUrl":"https://doi.org/10.1007/s12550-023-00489-z","url":null,"abstract":"<p><p>Deoxynivalenol is present in forage crops in concentrations that endanger animal welfare but is also found in cereal-based food. The amphipathic nature of mycotoxins allows them to cross the cell membrane and interacts with different cell organelles such as mitochondria and ribosomes. In our study, we investigated the gene expression of several genes in vivo and in vitro that are related to the metabolism. We observed a significantly higher COX5B and MHCII expression in enterocytes of DON-fed pigs compared to CON-fed pigs and a marked increase in GAPDH and SLC7A11 in DON-fed pigs, but we could not confirm this in vitro in IPEC-1. In vitro, functional metabolic analyses were performed with a seahorse analyzer. A significant increase of non-mitochondrial respiration was observed in all DON-treatment groups (50-2000 ng/mL). The oxygen consumption of cells, which were cultured on membranes, was examined with a fiber-glass electrode. Here, we found significantly lower values for DON 200- and DON 2000-treatment group. The effect on ribosomes was investigated using biorthogonal non-canonical amino acid tagging (BONCAT) to tag newly synthesized proteins. A significantly reduced amount was found in almost all DON-treatment groups. Our findings clearly show that apical and basolateral DON-treatment of epithelial cell layer results in decreasing amounts of newly synthesized proteins. Furthermore, our study shows that DON affects enterocyte metabolism in vivo and in vitro.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 3","pages":"219-231"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9930265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Fusaric acid detoxification: a strategy of Gliocladium roseum involved in its antagonism against Fusarium verticillioides.","authors":"Yi Kuang,&nbsp;Kirstin Scherlach,&nbsp;Christian Hertweck,&nbsp;Shengxiang Yang,&nbsp;Diego A Sampietro,&nbsp;Petr Karlovsky","doi":"10.1007/s12550-023-00481-7","DOIUrl":"https://doi.org/10.1007/s12550-023-00481-7","url":null,"abstract":"","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 3","pages":"317"},"PeriodicalIF":3.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10258785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root uptake and metabolization of Alternaria toxins by winter wheat plants using a hydroponic system.","authors":"Julia Jaster-Keller,&nbsp;Marina E H Müller,&nbsp;Ahmed H El-Khatib,&nbsp;Nicole Lorenz,&nbsp;Arnold Bahlmann,&nbsp;Ulrike Mülow-Stollin,&nbsp;Mirko Bunzel,&nbsp;Sophie Scheibenzuber,&nbsp;Michael Rychlik,&nbsp;Grit von der Waydbrink,&nbsp;Stefan Weigel","doi":"10.1007/s12550-023-00477-3","DOIUrl":"https://doi.org/10.1007/s12550-023-00477-3","url":null,"abstract":"<p><p>Fungi of the genus Alternaria are ubiquitous in the environment. Their mycotoxins can leach out of contaminated plants or crop debris into the soil entering the plant via the roots. We aim to evaluate the importance of this entry pathway and its contribution to the overall content of Alternaria toxins (ATs) in wheat plants to better understand the soil-plant-phytopathogen system. A hydroponic cultivation system was established and wheat plants were cultivated for up to two weeks under optimal climate conditions. One half of the plants was treated with a nutrient solution spiked with alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA), whereas the other half of the plants was cultivated without mycotoxins. Plants were harvested after 1 and 2 weeks and analyzed using a QuEChERS-based extraction and an in-house validated LC-MS/MS method for quantification of the ATs in roots, crowns, and leaves separately. ATs were taken up by the roots and transported throughout the plant up to the leaves after 1 as well as 2 weeks of cultivation with the roots showing the highest ATs levels followed by the crowns and the leaves. In addition, numerous AOH and AME conjugates like glucosides, malonyl glucosides, sulfates, and di/trihexosides were detected in different plant compartments and identified by high-resolution mass spectrometry. This is the first study demonstrating the uptake of ATs in vivo using a hydroponic system and whole wheat plants examining both the distribution of ATs within the plant compartments and the modification of ATs by the wheat plants.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 2","pages":"109-126"},"PeriodicalIF":3.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10181980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9455562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obtaining an aflatoxin-free and high-alcohol-content product using contaminated dried figs.","authors":"Seyda Senturk,&nbsp;Hakan Karaca","doi":"10.1007/s12550-023-00480-8","DOIUrl":"https://doi.org/10.1007/s12550-023-00480-8","url":null,"abstract":"<p><p>Dried fig is one of the most susceptible products to aflatoxin contamination. Since contaminated figs are not suitable for human consumption and cannot be used for any other purposes, they are burned in a chemical incinerator. In this study, we investigated the potential of using aflatoxin-contaminated dried figs as a raw material for ethanol production. For this purpose, contaminated dried figs (and also uncontaminated controls) were subjected to fermentation and subsequent distillation, and the alcohol and aflatoxin levels were determined during the processes. In addition, volatile by-products in the final product were determined using gas chromatography. Contaminated and uncontaminated figs had similar fermentation and distillation patterns. Although fermentation caused significant decreases in aflatoxin levels, there were still toxin residues in the fermented samples at the end of the process. On the other hand, aflatoxins were completely removed in the first step of the distillation. There were minor differences between the volatile compound compositions of the distillates produced from contaminated and uncontaminated figs. It was shown that obtaining aflatoxin-free and high-alcohol-content product using contaminated dried figs is possible according to the lab-scale conducted studies. Aflatoxin-contaminated dried figs can be used as a sustainable raw material for producing ethyl alcohol that can be used as an ingredient of surface disinfectants and/or fuel additive for vehicles.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 2","pages":"127-134"},"PeriodicalIF":3.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9456460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hazard characterisation for significant mycotoxins in food.","authors":"Frantisek Malir, Darina Pickova, Jakub Toman, Yann Grosse, Vladimir Ostry","doi":"10.1007/s12550-023-00478-2","DOIUrl":"10.1007/s12550-023-00478-2","url":null,"abstract":"<p><p>This review updates the current status of activities related to hazard characterisation for mycotoxins, with special reference to regulatory work accomplished within the European Union. Because the relevant information on these topics is widely scattered in the scientific literature, this review intends to provide a condensed overview on the most pertinent aspects. Human health risk assessment is a procedure to estimate the nature and potential for harmful effects of mycotoxins on human health due to exposure to them via contaminated food. This assessment involves hazard identification, hazard characterisation, exposure assessment, and risk characterisation. Mycotoxins covered in this review are aflatoxins, ochratoxin A, cyclopiazonic acid, citrinin, trichothecenes (deoxynivalenol, nivalenol, T-2, and HT-2 toxins), fumonisins, zearalenone, patulin, and ergot alkaloids. For mycotoxins with clear genotoxic/carcinogenic properties, the focus is on the margin of exposure approach. One of its goals is to document predictive characterisation of the human hazard, based on studies in animals using conditions of low exposure. For the other, non-genotoxic toxins, individual 'no adverse effect levels' have been established, but structural analogues or modified forms may still complicate assessment. During the process of hazard characterisation, each identified effect is assessed for human relevance. The estimation of a 'safe dose' is the hazard characterisation endpoint. The final aim of all of these activities is to establish a system, which is able to minimise and control the risk for the consumer from mycotoxins in food. Ongoing research on mycotoxins constantly comes up with new findings, which may have to be implemented into this system.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 2","pages":"81-93"},"PeriodicalIF":2.6,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9449220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycotoxin Research in the twenty-first century: the course for the future is set.","authors":"Ewald Usleber,&nbsp;Hans-Ulrich Humpf,&nbsp;Madeleine Plötz","doi":"10.1007/s12550-023-00475-5","DOIUrl":"https://doi.org/10.1007/s12550-023-00475-5","url":null,"abstract":"","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 1","pages":"1-2"},"PeriodicalIF":3.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10156619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10156563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutual effects on mycotoxin production during co-culture of ochratoxigenic and aflatoxigenic Aspergillus strains.","authors":"Chananya Chuaysrinule,&nbsp;Thanapoom Maneeboon,&nbsp;Warapa Mahakarnchanakul","doi":"10.1007/s12550-022-00473-z","DOIUrl":"https://doi.org/10.1007/s12550-022-00473-z","url":null,"abstract":"<p><p>Mycotoxin co-occurrence compromises the safety of food crops worldwide. Environmental factors, as well as fungal interaction, can substantially influence the infectivity of mycotoxigenic fungi and their subsequent production of multi-mycotoxin. Here, we investigated the mutual effects of the co-culture of ochratoxigenic and aflatoxigenic Aspergillus strains on the co-production of ochratoxin A (OTA) and aflatoxin B1 (AFB1). Single cultures of ochratoxigenic A. carbonarius and A. alliaceus grew optimally at 25 °C, whereas aflatoxigenic A. flavus grew optimally at 35 °C. The maximum levels of OTA and AFB1 were achieved at 25 °C, whereas mycotoxin production decreased at 35 °C. During competitive growth of the ochratoxigenic and aflatoxigenic isolates, inhibition or stimulation of mycotoxin production was dependent on the fungal strain, temperature, and the ratio of the spore concentration. Aspergillus carbonarius and A. alliaceus generally produced OTA, with similar patterns of relative OTA levels at all temperatures. AFB1 production by A. flavus in the presence of ochratoxigenic Aspergillus species was inhibited at 25 °C and stimulated at 35 °C. These results indicated that the temperature, presence of other mycotoxigenic Aspergillus species, and ratio of the initial spore concentration significantly contributed to the co-production of OTA and AFB1.</p>","PeriodicalId":19060,"journal":{"name":"Mycotoxin Research","volume":"39 1","pages":"57-66"},"PeriodicalIF":3.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9413651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}