Molecular Plant最新文献_第6页

NAD(H) and NADP(H) in plants and mammals. 植物和哺乳动物的NAD(H)和NADP(H)。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-06-02 Epub Date: 2025-05-13 DOI: 10.1016/j.molp.2025.05.004

Danying Lu, Murray Grant, Boon Leong Lim

{"title":"NAD(H) and NADP(H) in plants and mammals.","authors":"Danying Lu, Murray Grant, Boon Leong Lim","doi":"10.1016/j.molp.2025.05.004","DOIUrl":"10.1016/j.molp.2025.05.004","url":null,"abstract":"Nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) are essential metabolic coenzymes in prokaryotic and eukaryotic cells, with their reduced forms, NAD(P)H, serving as electron donors for myriad reactions. NADH is mainly involved in catabolic reactions, whereas NADPH is mainly involved in anabolic and antioxidative reactions. The presence of endosymbiont-derived organelles in eukaryotes has made the functional division of NADH and NADPH systems more complex. Chloroplasts in photoautotrophic eukaryotes provide additional sources of reductants, complicating the maintenance of the redox balance of NAD(P)+/NAD(P)H compared with heterotrophic eukaryotes. In this review, we discuss the two redox systems in plants and systematically compare them with those in mammals, including the similarities and differences in the biosynthesis and subcellular transport of NAD+, the biosynthesis of NADP+, and metabolic reactions for the reduction and oxidation of NAD(P)H. We also review the regulation of pyridine nucleotide pools and their ratios in different plant subcellular compartments and the effects of light on these ratios. We discuss the advantages of having both NADH and NADPH systems, highlight current gaps in our understanding of NAD(P)H metabolism, and propose research approaches that could fill in those gaps. The knowledge about NADH and NADPH systems could be used to guide bioengineering strategies to optimize redox-regulated processes and improve energy-use efficiency in crop plants.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"938-959"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12178909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A plant virus causes symptoms through the deployment of a host-mimicking protein domain to attract the insect vector. 植物病毒通过部署模仿宿主的蛋白质结构域来吸引昆虫媒介，从而引起症状。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-06-02 Epub Date: 2025-05-26 DOI: 10.1016/j.molp.2025.05.011

Man Gao, Emmanuel Aguilar, Borja Garnelo Gómez, Laura Medina-Puche, Pengfei Fan, Irene Ontiveros, Shaojun Pan, Huang Tan, Hua Wei, Edda von Roepenack-Lahaye, Na Chen, Xiao-Wei Wang, David C Baulcombe, Eduardo R Bejarano, Juan Antonio Díaz-Pendón, Masahiko Furutani, Miyo Terao Morita, Rosa Lozano-Durán

{"title":"A plant virus causes symptoms through the deployment of a host-mimicking protein domain to attract the insect vector.","authors":"Man Gao, Emmanuel Aguilar, Borja Garnelo Gómez, Laura Medina-Puche, Pengfei Fan, Irene Ontiveros, Shaojun Pan, Huang Tan, Hua Wei, Edda von Roepenack-Lahaye, Na Chen, Xiao-Wei Wang, David C Baulcombe, Eduardo R Bejarano, Juan Antonio Díaz-Pendón, Masahiko Furutani, Miyo Terao Morita, Rosa Lozano-Durán","doi":"10.1016/j.molp.2025.05.011","DOIUrl":"10.1016/j.molp.2025.05.011","url":null,"abstract":"Plant viruses cause symptoms with devastating consequences for agriculture. However, the molecular mechanisms underlying symptom development in viral infections remain largely unexplored. Here, we show that tomato yellow leaf curl virus (TYLCV) interferes with host developmental programs through a host-mimicking domain present in the viral C4 protein. This domain mediates the interaction between C4 and a family of RCC1-like domain-containing (RLD) proteins, previously shown to be required for proper plant development and environmental responses. C4 outcompetes an endogenous interactor of RLDs, hijacking RLD proteins to the plasma membrane and disrupting their function in orchestrating endomembrane trafficking and polar auxin transport. Strikingly, macroscopic symptoms do not affect viral accumulation in the plant but serve as attractants for the insect vector, presumably promoting pathogen spread in an ecological context. Our work sheds light on the molecular underpinnings and biological relevance of symptom development triggered by TYLCV in tomato. Since most plant viruses are insect-transmitted, the principles described here might have broad applicability to crop-virus interactions.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1029-1046"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A highly efficient and environmentally friendly strategy to obtain transgene-free genome-edited progeny in flowering plants. 一种在开花植物中获得无转基因基因组编辑后代的高效环保策略。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-06-02 Epub Date: 2025-03-24 DOI: 10.1016/j.molp.2025.03.014

Tianhe Cheng, Meng-Xiang Sun

引用次数: 0

Seed dormancy and germination in rice: Molecular regulatory mechanisms and breeding. 水稻种子休眠与萌发：分子调控机制及育种应用。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-06-02 Epub Date: 2025-05-26 DOI: 10.1016/j.molp.2025.05.010

Fan Xu, Hideki Yoshida, Chengcai Chu, Makoto Matsuoka, Jian Sun

{"title":"Seed dormancy and germination in rice: Molecular regulatory mechanisms and breeding.","authors":"Fan Xu, Hideki Yoshida, Chengcai Chu, Makoto Matsuoka, Jian Sun","doi":"10.1016/j.molp.2025.05.010","DOIUrl":"10.1016/j.molp.2025.05.010","url":null,"abstract":"The transition from dormancy to germination marks the initial stage of the plant life cycle, with its intensity, synchronicity, and timing being critical for crop growth, development, and adaptation to complex climate conditions. This review synthesizes recent advances with classic molecular mechanisms of dormancy and germination, including environmental responses and signaling cascades. We integrate these independent studies to provide a comprehensive perspective on the complex regulatory networks and discuss novel insights into how rice seeds perceive and respond to environmental cues during this transition, particularly focusing on stress tolerance to temperature and flooding. We aim to bridge the understanding of the molecular mechanisms of dormancy and germination with their breeding applications. Specifically, we discuss gene targets and feasible strategies for the genetic improvement of pre-harvest sprouting and direct-seeded rice, two key traits essential for climate resilience, both of which involve dormancy and germination. Finally, we propose the concept of engineering germination-smart varieties endowed with intelligent environmental adaptation.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"960-977"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The long noncoding RNA VIVIpary promotes seed dormancy release and pre-harvest sprouting through chromatin remodeling in rice. 长链非编码RNA viviparty通过染色质重塑促进水稻种子休眠释放和收获前发芽。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-06-02 Epub Date: 2025-04-23 DOI: 10.1016/j.molp.2025.04.010

Lu Yang, Yu Cheng, Chao Yuan, Yan-Fei Zhou, Qiao-Juan Huang, Wen-Long Zhao, Rui-Rui He, Jie Jiang, Yi-Chao Qin, Zheng-Tong Chen, Yu-Chan Zhang, Meng-Qi Lei, Jian-Ping Lian, Yue-Qin Chen

{"title":"The long noncoding RNA VIVIpary promotes seed dormancy release and pre-harvest sprouting through chromatin remodeling in rice.","authors":"Lu Yang, Yu Cheng, Chao Yuan, Yan-Fei Zhou, Qiao-Juan Huang, Wen-Long Zhao, Rui-Rui He, Jie Jiang, Yi-Chao Qin, Zheng-Tong Chen, Yu-Chan Zhang, Meng-Qi Lei, Jian-Ping Lian, Yue-Qin Chen","doi":"10.1016/j.molp.2025.04.010","DOIUrl":"10.1016/j.molp.2025.04.010","url":null,"abstract":"Seed dormancy enables seeds to remain dormant until the environmental conditions are ideal for germination. Understanding the molecular mechanisms that underlie seed dormancy is essential for improving grain quality and preventing pre-harvest sprouting (PHS), a major challenge in global agriculture. Here, we address how long noncoding RNAs (lncRNAs) contribute to the regulation of seed dormancy in rice (Oryza sativa). We identified an lncRNA, VIVIpary, that is specifically expressed in embryos and is associated with shortened seed dormancy. VIVIpary exhibits higher expression in a PHS-sensitive variety, and its overexpression induces PHS, whereas its knockdown delays germination. Mechanistically, VIVIpary promotes the release of seed dormancy by regulating abscisic acid (ABA) signaling. VIVIpary serves as a spatial organizer that shapes chromatin architecture by directly binding to the chromatin adaptor protein OsMSI1 and enhancing its interaction with the histone deacetylase OsHDAC1, thereby reducing chromatin accessibility and fine-tuning ABA signaling. VIVIpary is differentially expressed between wild and cultivated rice, with higher expression in japonica rice, suggesting that it was a target of selection during rice domestication. Together, our findings reveal a domestication-associated lncRNA that modulates ABA signaling and chromatin architecture to regulate seed dormancy and PHS in rice, providing a potential target for improvement of rice agronomic traits.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"978-994"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143973053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antisense-mediated regulation of nitric oxide biosynthesis. 一氧化氮生物合成的反义调控。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-17 DOI: 10.1016/j.molp.2025.03.010

Wil Prall, Martin Crespi

引用次数: 0

Elucidation and de novo reconstitution of glyceollin biosynthesis. 甘油生物合成的阐释和从头重构。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-04-10 DOI: 10.1016/j.molp.2025.04.003

Yunlong Sun, Cong Chen, Chao Lin, Hao Zhang, Jiazhang Lian, Benke Hong

{"title":"Elucidation and de novo reconstitution of glyceollin biosynthesis.","authors":"Yunlong Sun, Cong Chen, Chao Lin, Hao Zhang, Jiazhang Lian, Benke Hong","doi":"10.1016/j.molp.2025.04.003","DOIUrl":"https://doi.org/10.1016/j.molp.2025.04.003","url":null,"abstract":"Glyceollins are phytoalexins, soybean-produced compounds that respond to pathogen invasion, injury, and environmental challenges. While these compounds have diverse bioactivities, their limited accessibility hinders further physiological and biochemical studies. Additionally, the incomplete understanding of glyceollin biosynthesis, particularly cyclization steps, remains a major barrier to sustainable production through synthetic biology. In this study, we uncover the complete biosynthetic pathway of glyceollins through a combinatorial approach involving transient expression in Nicotiana benthamiana, in vitro enzyme characterization, and yeast feeding studies. We identified previously uncharacterized genes encoding reductases for 7,2',4'-trihydroxyisoflavanol biosynthesis and five P450 enzymes that mediate the final oxidative cyclization to produce glyceollins I, II, and III. By de novo reconstruction of the pathway through synthetic biology and metabolic engineering, we successfully produced glyceollins from simple carbon sources in baker's yeast. This work advances the understanding of glyceollin biosynthesis in soybeans, enables sustainable production in microbial hosts, and offers new opportunities for their application in agriculture and biology.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"18 5","pages":"820-832"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-cadmium rice for food safety: OsCS1 is a genetic breakthrough. 低镉大米促进食品安全：OsCS1是一项基因突破。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-25 DOI: 10.1016/j.molp.2025.03.013

Iqra Noor, Hamza Sohail, Biao Jin, Changquan Zhang

引用次数: 0

Two E-clade protein phosphatase 2Cs enhance ABA signaling by dephosphorylating ABI1 in Arabidopsis. 在拟南芥中，两个e枝蛋白磷酸酶2c通过去磷酸化ABI1来增强ABA信号。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-31 DOI: 10.1016/j.molp.2025.03.019

Ya Zhang, Liyuan Han, Junjie Liu, Miao Chang, Chuanling Li, Jian-Xiu Shang, Zhiping Deng, Wenqiang Tang, Yu Sun

{"title":"Two E-clade protein phosphatase 2Cs enhance ABA signaling by dephosphorylating ABI1 in Arabidopsis.","authors":"Ya Zhang, Liyuan Han, Junjie Liu, Miao Chang, Chuanling Li, Jian-Xiu Shang, Zhiping Deng, Wenqiang Tang, Yu Sun","doi":"10.1016/j.molp.2025.03.019","DOIUrl":"10.1016/j.molp.2025.03.019","url":null,"abstract":"ABA INSENSITIVE 1 (ABI1) and ABI2 are co-receptors of the phytohormone abscisic acid (ABA). Studies have demonstrated that phosphorylation of multiple amino acids on ABI1/2 augments their ability to inhibit ABA signaling in planta. However, whether and how the dephosphorylation of ABI1/2 is regulated to enhance plant sensitivity to ABA remain unknown. In this study, we identified two protein phosphatases, designated ABI1-Dephosphorylating E-clade PP2C 1 (ADEP1) and ADEP2, that interact with ABI1/2. Mutants lacking ADEP1, ADEP2, or both (adep1/2) exhibited reduced ABA inhibition of seed germination and root growth, as well as lower levels of ABA-induced stomatal closure. In addition, ABA-induced accumulation of ABI5 protein and expression of downstream target genes are reduced in the adep1/2 mutant compared with the wild type. These findings suggest that ADEP1/2 function as positive regulators of the ABA signaling pathway. Mass spectrometry analysis and two-dimensional electrophoresis identified Ser117 as a major ABA-induced phosphorylation site on the ABI1 protein. ADEP1/2 can dephosphorylate Ser117, leading to destabilization of the ABI1 protein and increased sensitivity of plants to ABA. Moreover, ABA treatment decreases the abundance of ADEP1/2 proteins. In summary, our study reveals two novel regulatory proteins that modulate ABA signaling and provides new insights into the regulatory network that fine-tunes plant ABA responses.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"783-796"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative spatial transcriptomics reveals root dryland adaptation mechanism in rice and HMGB1 as a key regulator. 比较空间转录组学揭示了水稻根系旱地适应机制和HMGB1作为关键调控因子的作用。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-04-06 DOI: 10.1016/j.molp.2025.04.001

Liyuan Zhong, Leping Geng, Yimeng Xiang, Xuanmin Guang, Le Cao, Jiawei Shi, Weikun Li, Jianglin Wang, Weiming He, Liyu Huang, Feng Yang, Yi-Xuan Bai, Sunil Kumar Sahu, Xing Guo, Shilai Zhang, Gengyun Zhang, Xun Xu, Fengyi Hu, Wanneng Yang, Huan Liu, Yu Zhao, Jun Lyu

{"title":"Comparative spatial transcriptomics reveals root dryland adaptation mechanism in rice and HMGB1 as a key regulator.","authors":"Liyuan Zhong, Leping Geng, Yimeng Xiang, Xuanmin Guang, Le Cao, Jiawei Shi, Weikun Li, Jianglin Wang, Weiming He, Liyu Huang, Feng Yang, Yi-Xuan Bai, Sunil Kumar Sahu, Xing Guo, Shilai Zhang, Gengyun Zhang, Xun Xu, Fengyi Hu, Wanneng Yang, Huan Liu, Yu Zhao, Jun Lyu","doi":"10.1016/j.molp.2025.04.001","DOIUrl":"10.1016/j.molp.2025.04.001","url":null,"abstract":"Drought severely threatens food security, and its detrimental effects will be exacerbated by climate change in many parts of the world. Rice production is water-consuming and particularly vulnerable to drought stress. Upland rice is a special rice ecotype that specifically adapts to dryland mainly due to its robust root system. However, the molecular and developmental mechanism underlying this adaption has remained elusive. In this study, by comparing the root development between upland and irrigated rice phenotypically and cytologically, we identified key developmental phenotypes that distinguish upland rice from irrigated rice. We further generated spatial transcriptomic atlases for coleoptilar nodes and root tips to explore their molecular differences in crown root formation and development, uncovering promising genes for enhancing rice drought resistance. Among the identified genes, HMGB1, a transcriptional regulator, functions as a key factor that facilitates root elongation and thickening in upland rice and thereby enhances drought resistance. In summary, our study uncovers spatially resolved transcriptomic features in roots of upland rice that contribute to its adaptation to dryland conditions, providing valuable genetic resources for breeding drought-resilient rice.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"797-819"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0