Molecular Plant最新文献_第6页

Plant-specific casein kinases phosphorylate and stabilize SMXL6/7/8 to suppress strigolactone signaling and promote shoot branching. 植物特异性酪蛋白激酶磷酸化和稳定SMXL6/7/8，抑制独角麦内酯信号，促进芽分枝。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-01 Epub Date: 2025-07-22 DOI: 10.1016/j.molp.2025.07.012

Xin Su, Hong-Wei Xue

{"title":"Plant-specific casein kinases phosphorylate and stabilize SMXL6/7/8 to suppress strigolactone signaling and promote shoot branching.","authors":"Xin Su, Hong-Wei Xue","doi":"10.1016/j.molp.2025.07.012","DOIUrl":"10.1016/j.molp.2025.07.012","url":null,"abstract":"Strigolactones (SLs) significantly impact agricultural production due to their central role in regulating plant morphology. As switch controllers of SL signaling, the transcriptional repressors, suppressor of MAX2 1-like 6/7/8 (SMXL6/7/8), are ubiquitinated by the F-box E3 ligase, more axillary growth 2 (MAX2) for degradation through the 26S proteasome, which is mediated by the receptor DWARF14. However, post-translational modifications and regulatory mechanisms of SMXL6/7/8 proteins remain unknown. Here, we demonstrate that Arabidopsis seedlings deficient in or overexpressing an evolutionarily conserved, plant-specific protein kinase Arabidopsis EL1-like (AEL1-4) exhibit significantly reduced or enhanced branching, respectively. Biochemical assays reveal that AEL interacts with and phosphorylates SMXL6/7/8 proteins, inhibiting their interactions with MAX2 and suppressing their degradation, thereby negatively interfering with SL-regulated branching. Notably, SL signaling reduces the expression of AEL genes dependent on action of SMXL6/7/8 and diminishes AEL-SMXL protein interactions. In summary, this study reveals the importance of protein phosphorylation in regulating SL signaling and its effects, highlighting a fine-tuning mechanism of SL signaling through phosphorylation-mediated transition between active and inactive forms of SMXL6/7/8.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1458-1471"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Varigraph: An accurate and widely applicable pangenome graph-based variant genotyper for diploid and polyploid genomes. 变异图：一个准确的和广泛适用的基于全基因组图谱的变异基因型，用于二倍体和多倍体基因组。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-01 Epub Date: 2025-08-05 DOI: 10.1016/j.molp.2025.08.001

Ze-Zhen Du, Jia-Bao He, Pei-Xuan Xiao, Jianbing Hu, Ning Yang, Wen-Biao Jiao

{"title":"Varigraph: An accurate and widely applicable pangenome graph-based variant genotyper for diploid and polyploid genomes.","authors":"Ze-Zhen Du, Jia-Bao He, Pei-Xuan Xiao, Jianbing Hu, Ning Yang, Wen-Biao Jiao","doi":"10.1016/j.molp.2025.08.001","DOIUrl":"10.1016/j.molp.2025.08.001","url":null,"abstract":"Accurate variant genotyping is crucial for genomics-assisted breeding. Graph pangenome references can address single-reference bias, thereby enhancing the performance of variant genotyping and empowering downstream applications in population genetics and quantitative genetics. However, existing pangenome-based genotyping methods are ineffective in handling large or complex pangenome graphs, particularly in polyploid genomes. Here, we introduce Varigraph, an algorithm that leverages the comparison of unique and repetitive k-mers between variant sites and short reads for genotyping both small and large variants. We evaluated Varigraph on a diverse set of representative plant genomes as well as human genomes. Varigraph outperforms current state-of-the-art linear and graph-based genotypers across non-human genomes while maintaining comparable genotyping performance in human genomes. By employing efficient data structures including counting Bloom filter and bitmap storage, as well as GPU models, Varigraph achieves improved precision and robustness in repetitive regions while managing computational costs for large datasets. Its wide applicability extends to highly repetitive or large genomes, such as those of maize and wheat. Significantly, Varigraph can handle extensive pangenome graphs, as demonstrated by its performance on a dataset containing 252 rice genomes, for which it achieved a precision exceeding 0.9 for both small and large variants. Notably, Varigraph is capable of effectively utilizing pangenome graphs for genotyping autopolyploids, enabling precise determination of allele dosage. In summary, this work provides a robust and accurate solution for genotyping plant genomes and will advance plant genomic studies and genomics-assisted breeding.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1587-1601"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis HSFA1b functions as a heat sensor inhibiting OST1-mediated stomatal closure through its adenylate-cyclase activity. 拟南芥HSFA1b作为热传感器，通过腺苷酸环化酶活性抑制ost1介导的气孔关闭。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-01 Epub Date: 2025-07-30 DOI: 10.1016/j.molp.2025.07.018

Yu Zhang, Ru-Feng Song, Xiao-Yu Hu, Hui Zhou, Wei Wang, Junli Zhang, Xin Zhang, Liu-Mei Li, Su-Ting Wang, Yuwei Song, Fuyou Xiang, Jingjing Xing, Yu Long, Changqing Zhang, José Ramón Botella, Guoyong An, Siyi Guo, Wen-Cheng Liu, Chun-Peng Song

{"title":"Arabidopsis HSFA1b functions as a heat sensor inhibiting OST1-mediated stomatal closure through its adenylate-cyclase activity.","authors":"Yu Zhang, Ru-Feng Song, Xiao-Yu Hu, Hui Zhou, Wei Wang, Junli Zhang, Xin Zhang, Liu-Mei Li, Su-Ting Wang, Yuwei Song, Fuyou Xiang, Jingjing Xing, Yu Long, Changqing Zhang, José Ramón Botella, Guoyong An, Siyi Guo, Wen-Cheng Liu, Chun-Peng Song","doi":"10.1016/j.molp.2025.07.018","DOIUrl":"10.1016/j.molp.2025.07.018","url":null,"abstract":"Global warming profoundly affects plant communities, but the mechanisms and the identity of sensors controlling plant thermosensing remain poorly understood. In this study, we identify the heat-shock factor A1b (HSFA1b) transcription factor as a heat sensor that regulates stomatal responses by inhibiting open stomata 1 (OST1) kinase activity in Arabidopsis. OST1 induces stomatal closure under heat stress independently of abscisic acid, and its activity is inhibited in the cytosol by the C-terminus of HSFA1b through intrinsic adenylate cyclase (AC) activity. Arabidopsis HSFA1b could complement an AC-deficient bacterium, and the cyclic AMP produced by HSFA1b could bind to and inhibit OST1 kinase activity. This inhibition is relieved under heat stress by HSFA1b translocation into the nucleus, coupling OST1 inhibition with the activation of heat-shock protein genes involved in the perception and signaling of high temperature. Collectively, our study demonstrates that HSFA1b functions as a heat sensor, inhibiting heat stress-induced and OST1-mediated stomatal closure through its AC activity.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1549-1566"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell wall pectins influence plasmodesmata permeability and, thereby, directionality. 细胞壁果胶影响胞间连丝的渗透性，从而影响其方向性。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-01 DOI: 10.1016/j.molp.2025.08.017

Johannes Liesche, Alexander Schulz

引用次数: 0

Designing Better Crops with Phased Pangenomes. 用分阶段泛基因组设计更好的作物。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-08-28 DOI: 10.1016/j.molp.2025.08.014

Qichao Lian, Wen-Biao Jiao, Yingxiang Wang

引用次数: 0

Simple but effective: Minimalist NLR rewiring, maximal virus resistance. 简单而有效：最少的NLR重新布线，最大的病毒抗性。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-08-27 DOI: 10.1016/j.molp.2025.08.007

Ming Wu, Shanshan Zhao, Jianguo Wu

引用次数: 0

The chloroplast translocon subunit TOC33 relays singlet oxygen-induced chloroplast-to-nucleus retrograde signaling in Arabidopsis 叶绿体转位亚基TOC33在拟南芥中传递单线态氧诱导的叶绿体到细胞核的逆行信号

IF 27.5 1区生物学

Molecular Plant Pub Date : 2025-08-21 DOI: 10.1016/j.molp.2025.08.013

Huan Zhao, Fan Zhang, Xinyue Wang, Kaiwei Liu, Lingling Zhang, Jingrui Li, Chanhong Kim, Liangsheng Wang

{"title":"The chloroplast translocon subunit TOC33 relays singlet oxygen-induced chloroplast-to-nucleus retrograde signaling in Arabidopsis","authors":"Huan Zhao, Fan Zhang, Xinyue Wang, Kaiwei Liu, Lingling Zhang, Jingrui Li, Chanhong Kim, Liangsheng Wang","doi":"10.1016/j.molp.2025.08.013","DOIUrl":"https://doi.org/10.1016/j.molp.2025.08.013","url":null,"abstract":"Chloroplasts engaged in oxygenic photosynthesis frequently overproduce reactive oxygen species (ROS) under stress conditions, with singlet oxygen (1O2) being particularly harmful due to its high reactivity and short lifespan. The nuclear-encoded chloroplast protein EXECUTER1 (EX1) senses elevated 1O2 levels through Trp643 oxidation and undergoes proteolysis, a process essential for activating 1O2-induced EX1-mediated chloroplast-to-nucleus retrograde signaling (1O2-EX1 signaling). However, the link between EX1 proteolysis and subsequent nuclear transcriptome changes remains unclear. In this study, we isolated SOF1 (suppressor of flu 1) through a forward genetic screen using EMS-mutagenized flu mutant seeds of Arabidopsis thaliana harboring Flag-fused EX1 driven by its native promoter (referred to as fluEX1). Like flu, fluEX1 plants conditionally produce 1O2 in chloroplasts upon a dark-to-light shift. In the fluEX1 sof1, all 1O2-induced stress responses are largely suppressed, despite 1O2 levels being similar to those in the fluEX1. SOF1 encodes the chloroplast outer envelope-anchored preprotein import receptor TOC33. While TOC33 loss does not affect EX1 import, abundance, localization, and 1O2-induced proteolysis in the chloroplast, it blocks 1O2-induced chloroplast-to-nucleus retrograde signaling. TOC33 interacts with the UVR domain of EX1 (EX1-UVR) in the chloroplast envelope, facilitating 1O2-induced decrease of the chloroplast EX1-UVR and increase of the nucleus EX1-UVR. Moreover, ectopic expression of EX1-UVR outside of the chloroplast bypasses the restrictive barrier imposed by the chloroplast envelope, activating 1O2 signaling and inducing stress responses. Our findings demonstrate that SOF1/TOC33 mediates 1O2-EX1 signaling from the chloroplast to the nucleus and that the EX1-UVR domain can substitute for full-length EX1 in this signaling pathway.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"52 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144900248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional and post-translational regulation of wheat TaWRKY40 orchestrates ROS-mediated plant resistance against stripe rust fungus 小麦TaWRKY40转录和翻译后调控调控了ros介导的植物对条锈病的抗性

IF 27.5 1区生物学

Molecular Plant Pub Date : 2025-08-21 DOI: 10.1016/j.molp.2025.08.012

Jiani He, Qiongqiong Li, Chenchen Liang, Ning Wang, Jianfeng Wang, Cong Jiang, Zhensheng Kang, Chunlei Tang, Xiaojie Wang

{"title":"Transcriptional and post-translational regulation of wheat TaWRKY40 orchestrates ROS-mediated plant resistance against stripe rust fungus","authors":"Jiani He, Qiongqiong Li, Chenchen Liang, Ning Wang, Jianfeng Wang, Cong Jiang, Zhensheng Kang, Chunlei Tang, Xiaojie Wang","doi":"10.1016/j.molp.2025.08.012","DOIUrl":"https://doi.org/10.1016/j.molp.2025.08.012","url":null,"abstract":"Reactive oxygen species (ROS) generated by respiratory burst oxidase homologs (RBOHs) are critical for plant immunity. Despite the known transcriptional and post-translational regulation of RBOHD activity, the dynamic control of the ROS burst during plant immune responses remains elusive. Here, we demonstrate that upon infection with avirulent <ce:italic>Puccinia striiformis</ce:italic> f. sp. <ce:italic>tritic</ce:italic>i (<ce:italic>Pst</ce:italic>) races, the wheat transcription factor <ce:italic>TaWRKY40</ce:italic> is activated, driving an extracellular ROS burst by binding to the promoter of <ce:italic>TaNOX10</ce:italic> (RBOHD) and transcriptionally activating it. Furthermore, TaWRKY40 undergoes phosphorylation by the brassinolide signaling kinase TaBSK3, which promotes TaWRKY40 nuclear translocation and enhances <ce:italic>TaNOX10</ce:italic> transactivation. This cascade elevates extracellular ROS levels, conferring resistance to stripe rust. Conversely, when encountering virulent <ce:italic>Pst</ce:italic> races, another wheat WRKY transcription factor, <ce:italic>TaWRKY19</ce:italic>, is upregulated, which transcriptionally suppresses <ce:italic>TaWRKY40</ce:italic> and competitively binds to the same <ce:italic>cis</ce:italic>-element in the <ce:italic>TaNOX10</ce:italic> promoter. This jointly inhibits <ce:italic>TaNOX10</ce:italic> expression, suppresses ROS accumulation, and renders wheat susceptible. Collectively, these findings reveal a transcriptional activation module comprising TaBSK3-TaWRKY40-TaNOX10 that governs ROS production and establish a TaWRKY19-TaWRKY40 dual regulatory module that fine-tunes ROS burst during wheat-<ce:italic>Pst</ce:italic> interactions. Importantly, this coordinated ROS regulation by TaWRKY19 and TaWRKY40 enables wheat to mount differential resistance against <ce:italic>Pst</ce:italic> races with distinct virulence levels.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"21 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144900249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reinventing a Sustainable Green Revolution by Breeding and Planting Semi-dwarf Maize 通过培育和种植半矮秆玉米来重塑可持续的绿色革命

IF 27.5 1区生物学

Molecular Plant Pub Date : 2025-08-21 DOI: 10.1016/j.molp.2025.08.011

Senlin Xiao, Wei Song, Ronghuan Wang, Shuang Zhang, Mengyuan Liu, Ruyang Zhang, Tianjun Xu, Hui Liu, Jie Hu, Jinfeng Xing, Xiaqing Wang, Tianyi Wang, Xiangdong Fu, Jiuran Zhao

{"title":"Reinventing a Sustainable Green Revolution by Breeding and Planting Semi-dwarf Maize","authors":"Senlin Xiao, Wei Song, Ronghuan Wang, Shuang Zhang, Mengyuan Liu, Ruyang Zhang, Tianjun Xu, Hui Liu, Jie Hu, Jinfeng Xing, Xiaqing Wang, Tianyi Wang, Xiangdong Fu, Jiuran Zhao","doi":"10.1016/j.molp.2025.08.011","DOIUrl":"https://doi.org/10.1016/j.molp.2025.08.011","url":null,"abstract":"The agricultural Green Revolution gave rise to semi-dwarf varieties that increased wheat and rice yields under nitrogen-intensive and high-density planting condition. However, maize, a cornerstone of global food security, never underwent an equivalent architectural improvement. During the 1940s–1960s, significant yield gains in maize had already been achieved via hybrid breeding. Nevertheless, the growing demand for high-density planting has exposed challenges in maize, such as lodging susceptibility and nutrient competition, consequently driving an urgent need for shorter hybrid maize varieties. Here, we summarize recent advances in maize height regulation, architectural optimization, and nitrogen use efficiency, which provide actionable targets for semi-dwarf maize breeding. Current biotechnology breakthroughs now enable the development of semi-dwarf maize varieties that enhance lodging resistance while reducing fertilizer dependency, thereby overcoming the historical limitations of Green Revolution varieties. We propose that semi-dwarf maize holds the potential to revolutionize maize production by enhancing resilience, yield, and sustainability.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"96 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144900250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural insights into a plant-conserved DHFR-TS reveal a selective herbicide target. 对植物保守的DHFR-TS的结构见解揭示了选择性除草剂靶标。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-08-04 Epub Date: 2025-06-30 DOI: 10.1016/j.molp.2025.06.016

Joel Haywood, Karen J Breese, Daniel P McDougal, Callum Verdonk, Abigail Partridge, Adrian F Lo, Jingjing Zhang, Wen-Chao Yang, John B Bruning, Kevin J Saliba, Charles S Bond, Keith A Stubbs, Joshua S Mylne

{"title":"Structural insights into a plant-conserved DHFR-TS reveal a selective herbicide target.","authors":"Joel Haywood, Karen J Breese, Daniel P McDougal, Callum Verdonk, Abigail Partridge, Adrian F Lo, Jingjing Zhang, Wen-Chao Yang, John B Bruning, Kevin J Saliba, Charles S Bond, Keith A Stubbs, Joshua S Mylne","doi":"10.1016/j.molp.2025.06.016","DOIUrl":"10.1016/j.molp.2025.06.016","url":null,"abstract":"Modern agricultural practices rely on herbicides to reduce yield losses. Herbicide-resistant weeds threaten herbicide utility and, hence, food security. New herbicide modes of action and integrated pest-management practices are vital to mitigate this threat. As the antimalarials that target the bifunctional enzyme dihydrofolate reductase-thymidylate synthase (DHFR-TS) have been shown to be herbicidal, DHFR-TS might represent a mode-of-action target for the development of herbicides. Here, we present the crystal structure of a DHFR-TS (AtDHFR-TS1) from the model dicot Arabidopsis thaliana. It shows a divergent DHFR active site and a linker domain that challenges previous classifications of bifunctional DHFR-TS proteins. This plant-conserved architecture enabled us to develop highly selective herbicidal inhibitors of AtDHFR-TS1 over human DHFR and identify inhibitors with unique scaffolds via a large-library virtual screen. These results suggest that DHFR-TS is a viable herbicide target.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1294-1309"},"PeriodicalIF":24.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0