Molecular Plant最新文献

Molecular and Cellular Mechanisms of Photoperiod and Thermo-sensitive Genic Male Sterility in Plants.

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-19 DOI: 10.1016/j.molp.2024.12.012

Na Wang, Xiang Li, Jun Zhu, Zhong-Nan Yang

{"title":"Molecular and Cellular Mechanisms of Photoperiod and Thermo-sensitive Genic Male Sterility in Plants.","authors":"Na Wang, Xiang Li, Jun Zhu, Zhong-Nan Yang","doi":"10.1016/j.molp.2024.12.012","DOIUrl":"https://doi.org/10.1016/j.molp.2024.12.012","url":null,"abstract":"Photo-thermosensitive genic male sterile (P/TGMS) lines display male sterility under high temperature/long-day light conditions, and male fertility under low temperature/short-day light conditions. P/TGMS lines are the fundamental basis for the two-line hybrid breeding, which has notably increased the yield potential and grain quality of rice cultivars. In this review, we focus on the research progress on photoperiod and thermosensitive genic male sterility in plants. The essence for P/TGMS line is their ability to produce viable pollen under varying conditions. We have provided an overview of the process involved in anther and pollen development, as well as the molecular, cellular and genetic mechanisms underlying P/TGMS in Arabidopsis, rice and other crops. Slow development has been identified as a common mechanism in both Arabidopsis and rice P/TGMS fertility restoration, while ROS homeostasis has been implicated in rice P/TGMS. Furthermore, we have discussed the prospective applications of P/TGMS and potential solutions to challenges in this field. This review deepens our understanding of the mechanisms underlying P/TGMS and its utilization in two-line hybrid breeding across diverse crops.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic, transcriptomic, and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin's orchid.

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-18 DOI: 10.1016/j.molp.2024.12.010

Kai Jiang, Birger Lindberg Møller, Shaofan Luo, Yu Yang, David R Nelson, Elizabeth Heather Jakobsen Neilson, Joachim Møller Christensen, Kai Hua, Chao Hu, Xinhua Zeng, Mohammed Saddik Motawie, Tao Wan, Guang-Wan Hu, Guy Eric Onjalalaina, Yijiao Wang, Juan Diego Gaitán-Espitia, Zhiwen Wang, Xiao-Yan Xu, Jiamin He, Linying Wang, Yuanyuan Li, Dong-Hui Peng, Siren Lan, Huiming Zhang, Qing-Feng Wang, Zhong-Jian Liu, Wei-Chang Huang

{"title":"Genomic, transcriptomic, and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin's orchid.","authors":"Kai Jiang, Birger Lindberg Møller, Shaofan Luo, Yu Yang, David R Nelson, Elizabeth Heather Jakobsen Neilson, Joachim Møller Christensen, Kai Hua, Chao Hu, Xinhua Zeng, Mohammed Saddik Motawie, Tao Wan, Guang-Wan Hu, Guy Eric Onjalalaina, Yijiao Wang, Juan Diego Gaitán-Espitia, Zhiwen Wang, Xiao-Yan Xu, Jiamin He, Linying Wang, Yuanyuan Li, Dong-Hui Peng, Siren Lan, Huiming Zhang, Qing-Feng Wang, Zhong-Jian Liu, Wei-Chang Huang","doi":"10.1016/j.molp.2024.12.010","DOIUrl":"https://doi.org/10.1016/j.molp.2024.12.010","url":null,"abstract":"Angraecum sesquipedale, also known as Darwin's orchid, possesses an exceptionally long nectar spur. Charles Darwin predicted the orchid to be pollinated by a hawkmoth with a correspondingly long proboscis, later identified as Xanthopan praedicta. In this plant-pollinator interaction, the A. sesquipedale flower emits a complex blend of scent compounds dominated by diurnally regulated oximes (R1R2C=N-OH) to attract crepuscular and nocturnal pollinators. The molecular mechanism of oxime biosynthesis remains unclear in orchids. Here, we present the chromosome-level genome of A. sesquipedale. The haploid genome size is 2.10 Gb and represents 19 pseudochromosomes. Cytochrome P450 encoding genes of the CYP79 family known to be involved in oxime biosynthesis in seed plants are not present in the A. sesquipedale genome nor in the genomes of other members of the orchid family. Metabolomic analysis of the A. sesquipedale flower revealed a substantial release of oximes at dusk during the blooming stage. By integrating metabolomic and transcriptomic correlation approaches, flavin-containing monooxygenases (FMOs) encoded by six tandem-repeat genes in the A. sesquipedale genome are identified as catalyzing the formation of oximes present. Further in vitro and in vivo assays confirm the function of FMOs in the oxime biosynthesis. We designate these FMOs as Orchid Oxime Synthases 1-6. The evolutionary aspects related to the CYP79 gene losses and neofunctionalization of FMO-catalyzed biosynthesis of oximes in Darwin's orchid provide new insights into the convergent evolution of biosynthetic pathways.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N⁶-methyladenosine on the natural antisense transcript of NIA1 stabilizes its mRNA to boost NO biosynthesis and modulate stomatal movement.

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-17 DOI: 10.1016/j.molp.2024.12.011

Jie Li, Wen Tian, Ting Chen, Qing-Yan Liu, Hua-Wei Wu, Chuan-Hui Liu, Yuan-Yuan Fang, Hui-Shan Guo, Jian-Hua Zhao

{"title":"N6-methyladenosine on the natural antisense transcript of NIA1 stabilizes its mRNA to boost NO biosynthesis and modulate stomatal movement.","authors":"Jie Li, Wen Tian, Ting Chen, Qing-Yan Liu, Hua-Wei Wu, Chuan-Hui Liu, Yuan-Yuan Fang, Hui-Shan Guo, Jian-Hua Zhao","doi":"10.1016/j.molp.2024.12.011","DOIUrl":"https://doi.org/10.1016/j.molp.2024.12.011","url":null,"abstract":"Nitric oxide (NO) is a crucial signaling molecule that regulates a wide range of metabolic pathways in different strata of organisms. In plants, nitrate reductase (NR) is a key enzyme for NO biosynthesis. There are two NR-encoding genes in Arabidopsis, NIA1 and NIA2, which are precisely regulated and expressed in a tissue-specific manner. Here, we found that the natural antisense transcript as-NIA1, transcribed from the 3' UTR of NIA1, stabilizes NIA1 mRNA to maintain its circadian oscillation in plants grown under the light/dark cycle. Importantly, as-NIA1-dependent NIA1 mRNA stability is indispensable for NIA1-mediated NO biosynthesis in guard cells and natural stomatal closure. Moreover, we revealed that PTB3 regulates the stabilization of NIA1 mRNA by directly binding to UC-rich elements of as-NIA1. We further found that MTA deposits N6-methyladenosine (m6A) on as-NIA1, facilitating the as-NIA1-PTB3 interaction in vivo, in agreement with RNA structure prediction in that m6A-mediated structural alterations expose the UC-rich elements whereby enhancing the accessibility of PTB3. Our findings reveal a mechanism by which plants precisely manipulate NO biosynthesis to modulate light/dark-regulated stomatal movement, expanding the understanding of the coupling of RNA epigenetic modifications and structures shaping RNA‒protein interactions.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Barley2035: A decade vision on barley research and breeding. 大麦 2035：大麦研究和育种十年展望。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-16 DOI: 10.1016/j.molp.2024.12.009

Congcong Jiang, Jinhong Kan, Guangqi Gao, Christoph Dockter, Chengdao Li, Wenxue Wu, Ping Yang, Nils Stein

{"title":"Barley2035: A decade vision on barley research and breeding.","authors":"Congcong Jiang, Jinhong Kan, Guangqi Gao, Christoph Dockter, Chengdao Li, Wenxue Wu, Ping Yang, Nils Stein","doi":"10.1016/j.molp.2024.12.009","DOIUrl":"https://doi.org/10.1016/j.molp.2024.12.009","url":null,"abstract":"Barley (Hordeum vulgare ssp. vulgare) is one of the oldest founder crops in early human civilization, and has been widely dispersed around the globe to supply human life through livestock feeding and brewing industries. It has been used in innovative research of cytogenetics, biochemistry, and genetics since the early half of the 20th century, facilitated by its mode of reproduction through self-pollination, its true diploid status which has contributed to the accumulation of a plethora of germplasm and mutant resources. Coming to the era of molecular genomics and biology, a multitude of barley genes and their involved regulatory mechanisms have been uncovered and functionally validated, providing the paradigm for equivalent studies in other Triticeae crops. This review features the advancements over the past decade in barley research, mainly regarding genomics and genomics-assisted germplasm exploration, genetic dissection of developmental and adaptation associated traits, as well as the complex dynamics of yield and quality formation. For the coming decade, the perspective of integration of these innovations in barley research and breeding is promising. Barley is proposed as a reference in Triticeae crops for new gene discovery, functional validation and molecular mechanism dissection. The application of precise genome editing as well as genomic prediction and selection, further enhanced by artificial intelligence-enforced tools and applications, is expected to boost barley improvement, in order to efficiently meet the evolving global demands for this important crop.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How ignoring market-informed crop varietal development fuels food price volatility. 忽视以市场为导向的作物品种开发是如何加剧粮食价格波动的？

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-12 DOI: 10.1016/j.molp.2024.12.008

Ganesh Alagarasan

引用次数: 0

ALFIN-like proteins link histone H3K4me3 to H2A ubiquitination and coordinate diverse chromatin modifications in Arabidopsis. ALFIN 样蛋白将组蛋白 H3K4me3 与 H2A 泛素化联系起来，并协调拟南芥中的多种染色质修饰。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-11 DOI: 10.1016/j.molp.2024.12.007

Xiao-Min Su, Dan-Yang Yuan, Na Liu, Zhao-Chen Zhang, Minqi Yang, Lin Li, She Chen, Yue Zhou, Xin-Jian He

{"title":"ALFIN-like proteins link histone H3K4me3 to H2A ubiquitination and coordinate diverse chromatin modifications in Arabidopsis.","authors":"Xiao-Min Su, Dan-Yang Yuan, Na Liu, Zhao-Chen Zhang, Minqi Yang, Lin Li, She Chen, Yue Zhou, Xin-Jian He","doi":"10.1016/j.molp.2024.12.007","DOIUrl":"https://doi.org/10.1016/j.molp.2024.12.007","url":null,"abstract":"Trimethylation of histone H3K4 (H3K4me3) is widely distributed at numerous actively transcribed protein-coding genes throughout the genome. However, the interplay between H3K4me3 and other chromatin modifications in plants remains poorly understood. In this study, we find that the Arabidopsis thaliana ALFIN-LIKE (AL) proteins contain a C-terminal PHD finger capable of binding to H3K4me3, along with a PHD-associated AL (PAL) domain that interacts with components of the Polycomb repressive complex 1 (PRC1), thereby facilitating H2A ubiquitination (H2Aub) at H3K4me3-enriched genes throughout the genome. Furthermore, we demonstrate that the loss of SDG2, a key histone H3K4 methyltransferase, leads to a reduction in H3K4me3 level, which subsequently causes a decrease in H2Aub on a genome-wide scale, revealing a strong association between H3K4me3 and H2Aub. Additionally, we show that the PAL domain interacts with various other chromatin-related proteins or complexes, including those involved in regulating H2A.Z deposition, H3K27me3 demethylation, histone deacetylation, and chromatin accessibility. Our genome-wide analysis suggests that the AL proteins play a crucial role in coordinating H3K4me3 with multiple other chromatin modifications across the genome.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The long noncoding RNA ALEX1 confers a functional phase state of ARF3 to enhance rice resistance to bacterial pathogens.

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-09 DOI: 10.1016/j.molp.2024.12.005

Meng-Qi Lei, Rui-Rui He, Yan-Fei Zhou, Lu Yang, Zhen-Fei Zhang, Chao Yuan, Wen-Long Zhao, Yu Cheng, Jian-Ping Lian, Yu-Chan Zhang, Wen-Tao Wang, Yang Yu, Yue-Qin Chen

{"title":"The long noncoding RNA ALEX1 confers a functional phase state of ARF3 to enhance rice resistance to bacterial pathogens.","authors":"Meng-Qi Lei, Rui-Rui He, Yan-Fei Zhou, Lu Yang, Zhen-Fei Zhang, Chao Yuan, Wen-Long Zhao, Yu Cheng, Jian-Ping Lian, Yu-Chan Zhang, Wen-Tao Wang, Yang Yu, Yue-Qin Chen","doi":"10.1016/j.molp.2024.12.005","DOIUrl":"https://doi.org/10.1016/j.molp.2024.12.005","url":null,"abstract":"Rice bacterial blight is a devastating disease worldwide, causing significant yield losses. Understanding how plants defend against microbial infection is critical for sustainable crop production. We previously identified a pathogen-induced long noncoding RNA (ALEX1). In this study, we show that ALEX1 localizes to the nucleus and directly binds to AUXIN RESPONSE FACTOR 3 (ARF3). We demonstrate that ARF3 forms condensates in the nucleus via its intrinsically disordered middle region (MR). Notably, ARF3 condensates takes on solid-like properties. We further revealed that ALEX1 directly binds to the MR of ARF3, regulating ARF3 condensate dynamics and promotes ARF3 homodimerization. The dispersed and dimeric form of ARF3, referred to as its functional phase state, enhances its ability to transcriptionally repress downstream target gene such as JAZ13, thereby modulating the jasmonic acid (JA) signaling pathway and strengthening pathogen resistance in rice. This study highlights the role of a long noncoding RNA in regulating protein condensation and assembly, contributing to pathogen defense in plants.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PDLLMs: A group of tailored DNA large language models for analyzing plant genomes.

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-09 DOI: 10.1016/j.molp.2024.12.006

Guanqing Liu, Long Chen, Yuechao Wu, Yangshuo Han, Yu Bao, Tao Zhang

引用次数: 0

Histone H4K8hib modification promotes gene expression and regulates rice immunity.

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-06 DOI: 10.1016/j.molp.2024.12.003

Qiutao Xu, Xuan Ma, Xuelu Wei, Zhengting Chen, Yuhang Duan, Yuliang Ju, Zhaoyun Wang, Jing Chen, Lu Zheng, Xiaolin Chen, Junbin Huang, Jisen Zhang, Xiaoyang Chen

引用次数: 0

Thermotolerance Through Trade-Off: Decapping WUSCHEL mRNA in Plant Stem Cells.

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-05 DOI: 10.1016/j.molp.2024.12.004

P Debnath, J J Olas

引用次数: 0