Molecular Plant最新文献

A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice. TT1-SCE1 模块整合了泛素化和 SUMOylation，以调控水稻的耐热性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-16 DOI: 10.1016/j.molp.2024.11.007

Hong-Xiao Yu, Ying-Jie Cao, Yi-Bing Yang, Jun-Xiang Shan, Wang-Wei Ye, Nai-Qian Dong, Yi Kan, Huai-Yu Zhao, Zi-Qi Lu, Shuang-Qin Guo, Jie-Jie Lei, Ben Liao, Hong-Xuan Lin

{"title":"A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice.","authors":"Hong-Xiao Yu, Ying-Jie Cao, Yi-Bing Yang, Jun-Xiang Shan, Wang-Wei Ye, Nai-Qian Dong, Yi Kan, Huai-Yu Zhao, Zi-Qi Lu, Shuang-Qin Guo, Jie-Jie Lei, Ben Liao, Hong-Xuan Lin","doi":"10.1016/j.molp.2024.11.007","DOIUrl":"https://doi.org/10.1016/j.molp.2024.11.007","url":null,"abstract":"Heat stress poses a significant threat to grain yield. Our previous study identified TT1, which encodes an α2 subunit of the 26S proteasome, as a critical regulator for rice heat tolerance, representing the first cloned QTL for crop heat tolerance. However, the mechanisms mediated by TT1 still remained elusive. In this study, we unveil SUMO-conjugating enzyme 1 (SCE1), which interacts with TT1 and acts as a downstream component of TT1, engaging in the TT1-mediated 26S proteasome degradation. SCE1 functions as a negative regulator of heat tolerance and can be linked to ubiquitination modification. Additionally, we observed that sHSPs such as Hsp24.1 and Hsp40 can undergo SUMOylation mediated by SCE1, leading to increased accumulation of sHSPs in the absence of SCE1. Furthermore, we propose that the global SUMOylation modulated by SCE1 serves as a crucial signal in response to heat stress, and the rapid decline in elevated SUMOylation is considered a positive effect to enhance heat tolerance due to the loss of SCE1 gene function. Reducing protein levels of SCE1 significantly enhanced grain yield under high-temperature stress by improving seed-setting rate and rice grain filling capacity. Our results uncover the critical role of SCE1 in TT1-mediated heat tolerance pathway, regulating the abundance of sHSP proteins and SUMOylation, and ultimately impacting rice heat tolerance. These findings underscore the significant potential of the TT1-SCE1 module in improving the heat tolerance of crops.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Branching out: nitrogen-dependent modulation of strigolactone signalling. 分支：氮依赖性地调节绞股蓝内酯信号。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-14 DOI: 10.1016/j.molp.2024.11.006

Andrew J Tuckey, Mark T Waters

引用次数: 0

The long non-coding RNA CARMA directs sucrose-responsive osmoregulation. 长非编码 RNA CARMA 引导蔗糖反应性渗透调节。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-12 DOI: 10.1016/j.molp.2024.11.005

Seungchul Lee, Ildoo Hwang

引用次数: 0

Volatilome-based GWAS identifies OsWRKY19 and OsNAC021 as key regulators of rice aroma. 基于挥发物的 GWAS 发现 OsWRKY19 和 OsNAC021 是水稻香气的关键调控因子。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-11 DOI: 10.1016/j.molp.2024.11.002

Yan Li, Yuanyuan Miao, Honglun Yuan, Fengkun Huang, Mingqi Sun, Liqiang He, Xianqing Liu, Jie Luo

{"title":"Volatilome-based GWAS identifies OsWRKY19 and OsNAC021 as key regulators of rice aroma.","authors":"Yan Li, Yuanyuan Miao, Honglun Yuan, Fengkun Huang, Mingqi Sun, Liqiang He, Xianqing Liu, Jie Luo","doi":"10.1016/j.molp.2024.11.002","DOIUrl":"https://doi.org/10.1016/j.molp.2024.11.002","url":null,"abstract":"Aromatic rice is globally favored for its distinctive scent, not only increasing nutritional value but also enhancing economic importance. However, apart from 2-acetyl-1-pyrroline (2-AP), the metabolic basis of aroma remains elusive, and the genetic underlying of the accumulation of fragrance metabolites are largely unknown. Here, we revealed 2-AP and fatty acid-derived volatile metabolites (FAVs) as key contributors to rice aroma by combining aroma rating with molecular docking. Using volatilome-based GWAS, we identified two regulatory genes that determine the natural variation of these fragrance metabolites. We demonstrated that OsWRKY19 not only enhances fragrance by negatively regulating OsBADH2 but also promotes agricultural traits in rice. Additionally, we revealed OsNAC021 that negatively regulates FAVs through the LOX pathway, and the knockout of it resulted in the over-accumulation of grain FAVs without a yield penalty. Our findings provide a compelling example of deciphering the genetic regulatory mechanisms underlying rice fragrance and pave the way for the creation of aromatic rice varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A metabolic roadmap of waxy corn flavor. 蜡质玉米风味的代谢路线图

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-11 DOI: 10.1016/j.molp.2024.11.003

Jingyun Luo, Chunmei He, Shijuan Yan, Chenglin Jiang, An Chen, Kun Li, Yongli Zhu, Songtao Gui, Ning Yang, Yingjie Xiao, Shenshen Wu, Fajun Zhang, Tieshan Liu, Juan Wang, Wenjie Huang, Yanhua Yang, Haiyan Wang, Wenyu Yang, Wenqiang Li, Lin Zhuo, Alisdair R Fernie, Junpeng Zhan, Liming Wang, Jianbing Yan

{"title":"A metabolic roadmap of waxy corn flavor.","authors":"Jingyun Luo, Chunmei He, Shijuan Yan, Chenglin Jiang, An Chen, Kun Li, Yongli Zhu, Songtao Gui, Ning Yang, Yingjie Xiao, Shenshen Wu, Fajun Zhang, Tieshan Liu, Juan Wang, Wenjie Huang, Yanhua Yang, Haiyan Wang, Wenyu Yang, Wenqiang Li, Lin Zhuo, Alisdair R Fernie, Junpeng Zhan, Liming Wang, Jianbing Yan","doi":"10.1016/j.molp.2024.11.003","DOIUrl":"https://doi.org/10.1016/j.molp.2024.11.003","url":null,"abstract":"As well as being a popular vegetable crop worldwide, waxy corn represents an important amylopectin source. However, little is known about the breeding history and flavor characteristics of this crop. In this study, comparative-omic analyses between 318 diverse waxy corn and 507 representative field corn inbred lines revealed that many metabolic pathways and genes exhibited characteristics of selection during the breeding history of waxy corn, contributing to the divergence between waxy and field corn. We show that waxy corn is not only altered in its glutinous property, but that sweetness, aroma, and palatability are all significantly affected. A substantial proportion (43%) of flavor-related metabolites have pleiotropic effects, impacting both flavor and yield characteristics and 27% of these metabolites are related to antagonistic outcomes on yield and flavor. Furthermore, we demonstrated, through multiple concrete examples, how yield and quality are coordinately or antagonistically regulated at the genetic level. In particular, we identified some sweet molecules such as DIMBOA and raffinose, that do not participate in the starch biosynthesis pathway, as potential targets for breeding a new type of \"sweet-waxy\" corn. Our findings shed light on the historical selection of waxy corn and demonstrate the genetic and metabolic basis of waxy corn flavor, thereby collectively providing valuable resources and knowledge for future crop breeding for improved nutritional quality.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new gene for restoring wild abortive-type cytoplasmic male sterility in rice. 恢复水稻野生败育型细胞质雄性不育的新基因

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-09 DOI: 10.1016/j.molp.2024.11.004

Xiaoming Zheng, Jian Sun, Cheng Cheng, Qian Qian

引用次数: 0

Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution. 两种益母草的多组分分析揭示益母草生物合成及其进化。

IF 27.5 1区生物学

Molecular Plant Pub Date : 2024-01-01 Epub Date: 2023-11-10 DOI: 10.1016/j.molp.2023.11.003

Peng Li, Meng-Xiao Yan, Pan Liu, Dan-Jie Yang, Ze-Kun He, Yun Gao, Yan Jiang, Yu Kong, Xin Zhong, Sheng Wu, Jun Yang, Hong-Xia Wang, Yan-Bo Huang, Le Wang, Xiao-Ya Chen, Yong-Hong Hu, Qing Zhao, Ping Xu

{"title":"Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution.","authors":"Peng Li, Meng-Xiao Yan, Pan Liu, Dan-Jie Yang, Ze-Kun He, Yun Gao, Yan Jiang, Yu Kong, Xin Zhong, Sheng Wu, Jun Yang, Hong-Xia Wang, Yan-Bo Huang, Le Wang, Xiao-Ya Chen, Yong-Hong Hu, Qing Zhao, Ping Xu","doi":"10.1016/j.molp.2023.11.003","DOIUrl":"10.1016/j.molp.2023.11.003","url":null,"abstract":"The Lamiaceae family is renowned for its terpenoid-based medicinal components, but Leonurus, which has traditional medicinal uses, stands out for its alkaloid-rich composition. Leonurine, the principal active compound found in Leonurus, has demonstrated promising effects in reducing blood lipids and treating strokes. However, the biosynthetic pathway of leonurine remains largely unexplored. Here, we present the chromosome-level genome sequence assemblies of Leonurus japonicus, known for its high leonurine production, and Leonurus sibiricus, characterized by very limited leonurine production. By integrating genomics, RNA sequencing, metabolomics, and enzyme activity assay data, we constructed the leonurine biosynthesis pathway and identified the arginine decarboxylase (ADC), uridine diphosphate glucosyltransferase (UGT), and serine carboxypeptidase-like (SCPL) acyltransferase enzymes that catalyze key reactions in this pathway. Further analyses revealed that the UGT-SCPL gene cluster evolved by gene duplication in the ancestor of Leonurus and neofunctionalization of SCPL in L. japonicus, which contributed to the accumulation of leonurine specifically in L. japonicus. Collectively, our comprehensive study illuminates leonurine biosynthesis and its evolution in Leonurus.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"158-177"},"PeriodicalIF":27.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72210128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adenosine monophosphate enhances callus regeneration competence for de novo plant organogenesis. 一磷酸腺苷增强愈伤组织再生能力，促进植物器官新生。

IF 27.5 1区生物学

Molecular Plant Pub Date : 2023-12-04 Epub Date: 2023-11-02 DOI: 10.1016/j.molp.2023.10.004

Hong Gil Lee, Seo Young Jang, Eun Yee Jie, Seung Hee Choi, Ok-Sun Park, Soon Hyung Bae, Hyun-Soon Kim, Suk Weon Kim, Geum-Sook Hwang, Pil Joon Seo

引用次数: 0

Roots dOdge streSs. 根茎躲避压力。

IF 27.5 1区生物学

Molecular Plant Pub Date : 2023-12-04 Epub Date: 2023-10-24 DOI: 10.1016/j.molp.2023.10.013

Stephan Clemens

引用次数: 0

Single-cell RNA sequencing reveals a hierarchical transcriptional regulatory network of terpenoid biosynthesis in cotton secretory glandular cells. 单细胞RNA测序揭示了棉花分泌腺细胞萜类生物合成的分级转录调控网络。

IF 27.5 1区生物学

Molecular Plant Pub Date : 2023-12-04 Epub Date: 2023-10-17 DOI: 10.1016/j.molp.2023.10.008

Jia-Ling Lin, Longxian Chen, Wen-Kai Wu, Xiao-Xiang Guo, Cheng-Hui Yu, Min Xu, Gui-Bin Nie, Jun-Ling Dun, Yan Li, Baofu Xu, Ling-Jian Wang, Xiao-Ya Chen, Wei Gao, Jin-Quan Huang

{"title":"Single-cell RNA sequencing reveals a hierarchical transcriptional regulatory network of terpenoid biosynthesis in cotton secretory glandular cells.","authors":"Jia-Ling Lin, Longxian Chen, Wen-Kai Wu, Xiao-Xiang Guo, Cheng-Hui Yu, Min Xu, Gui-Bin Nie, Jun-Ling Dun, Yan Li, Baofu Xu, Ling-Jian Wang, Xiao-Ya Chen, Wei Gao, Jin-Quan Huang","doi":"10.1016/j.molp.2023.10.008","DOIUrl":"10.1016/j.molp.2023.10.008","url":null,"abstract":"Plants can synthesize a wide range of terpenoids in response to various environmental cues. However, the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive. In this study, we employed single-cell RNA sequencing to comprehensively characterize the transcriptome profile of cotton leaves and established a hierarchical transcriptional network regulating cell-specific terpenoid production. We observed substantial expression levels of genes associated with the biosynthesis of both volatile terpenes (such as β-caryophyllene and β-myrcene) and non-volatile gossypol-type terpenoids in secretory glandular cells. Moreover, two novel transcription factors, namely GoHSFA4a and GoNAC42, are identified to function downstream of the Gossypium PIGMENT GLAND FORMATION genes. Both transcription factors could directly regulate the expression of terpenoid biosynthetic genes in secretory glandular cells in response to developmental and environmental stimuli. For convenient retrieval of the single-cell RNA sequencing data generated in this study, we developed a user-friendly web server . Our findings not only offer valuable insights into the precise regulation of terpenoid biosynthesis genes in cotton leaves but also provide potential targets for cotton breeding endeavors.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1990-2003"},"PeriodicalIF":27.5,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41237007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0