Molecular Plant最新文献

{"title":"A highly efficient and environmentally friendly strategy to obtain transgene-free genome-edited progeny in flowering plants.","authors":"Tianhe Cheng, Meng-Xiang Sun","doi":"10.1016/j.molp.2025.03.014","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.014","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SPOTLIGHT: Design of future climate smart crops by engineering heat stress-responsive gene expression.","authors":"Alisdair R Fernie, Mustafa Bulut","doi":"10.1016/j.molp.2025.03.012","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.012","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic transcriptome and GWAS uncover that a hydroxyproline-rich glycoprotein suppresses Agrobacterium-mediated transformation in maize.","authors":"Min Liu, Yan Yang, Tianhu Liang, Fengxia Hou, Minyan Zhang, Shijiang He, Peng Liu, Chaoying Zou, Langlang Ma, Guangtang Pan, Yaou Shen","doi":"10.1016/j.molp.2025.03.011","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.011","url":null,"abstract":"<p><p>Genetic transformation is a crucial tool for investigating gene function and advancing molecular breeding in crops, with Agrobacterium tumefaciens-mediated transformation being the primary method for plant genetic modification. However, this approach exhibits significant genotypic dependence in maize. Therefore, to overcome these limitations, we herein combined dynamic transcriptome analysis and genome-wide association study (GWAS) to identify the key genes controlling Agrobacterium infection frequency (AIF) in immature maize embryos. Transcriptome analysis of Agrobacterium-infected embryos uncovered 8483 and 1580 genotype-specific response genes in 18-599R (low AIF) and A188 (high AIF), respectively. A weighted gene co-expression network analysis (WGCNA) further revealed five and seven stage-specific co-expression modules in each corresponding line. Basd on a self-developed AIF quantitation method, the GWAS revealed 30 AIF-associated single nucleotide polymorphisms and 315 candidate genes under multiple environments. Integration of GWAS and WGCNA further identified 12 key genes associated with high AIF in A188, among which, ZmHRGP, encoding a hydroxyproline-rich glycoprotein, was functionally validated as a key factor of AIF in immature embryos. Knockout of ZmHRGP further enabled to establish a high-efficiency genetic transformation system for the 18-599R line, with the transformation frequency being approximately 80%. Moreover, transient reduction of ZmHRGP expression significantly enhanced the AIF of maize calluses and leaves. Overall, these findings advance our understanding of plant factors controlling Agrobacterium infection and contribute to the development of more efficient Agrobacterium-mediated transformation systems in crops.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antisense-mediated regulation of nitric oxide biosynthesis.","authors":"Wil Prall, Martin Crespi","doi":"10.1016/j.molp.2025.03.010","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.010","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Propagation of plant immunity via interactions between PRIMER and bystander cells.","authors":"Hongyuan Zheng, Yu Wang, Xuemin Zhou, Daowen Wang, Shi Xiao, Zheng Qing Fu","doi":"10.1016/j.molp.2025.03.009","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.009","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large-scale genomic and phenomic analyses of modern cultivars empower future rice breeding design.","authors":"Xiaoding Ma, Hao Wang, Shen Yan, Chuanqing Zhou, Kunneng Zhou, Qiang Zhang, Maomao Li, Yaolong Yang, Danting Li, Peng Song, Cuifeng Tang, Leiyue Geng, Jianchang Sun, Zhiyuan Ji, Xianjun Sun, Yongli Zhou, Peng Zhou, Di Cui, Bing Han, Xin Jing, Qiang He, Wei Fang, Longzhi Han","doi":"10.1016/j.molp.2025.03.007","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.007","url":null,"abstract":"<p><p>Modern cultivated rice plays a pivotal role in global food security. China accounts for nearly 30% of the world's rice production and has bred numerous cultivated varieties over the last decades that are well adapted to diverse growing regions. However, the genomic bases that underlie the phenotypes of modern cultivars are poorly characterized, limiting access to this vast resource for breeding of specialized, regionally adapted cultivars. In this study, we constructed a comprehensive genetic variation map of modern rice using resequencing datasets from 6044 representative cultivars from five major growing regions in China. Genomic and phenotypic analyses of this diversity panel revealed regional preferences for genomic backgrounds and specific traits, such as heading date, biotic/abiotic stress resistance, and grain shape, associated with adaptation to local growing conditions and consumer preferences. We identified 3131 QTLs associated with 53 phenotypes across 212 datasets under different environmental conditions through genome-wide association studies. Notably, we cloned and functionally verified a novel gene related to grain length, OsGL3.6. By integrating multiple datasets, we developed RiceAtlas, a versatile multi-scale toolkit for rice breeding design. We rapidly improved the grain shape of the Suigeng4 cultivar using the RiceAtlas breeding design function. These valuable resources enhance our understanding of the adaptability and breeding requirements of modern rice and can facilitate advances in future rice-breeding initiatives.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single plant NLR is able to recognize effectors from a wide range of adapted and non-adapted pathogens.","authors":"Soohyun Oh, Doil Choi","doi":"10.1016/j.molp.2025.03.008","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.008","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mobilizing Triticeae diversity from Genebanks to farmer's field.","authors":"Awais Rasheed, Jindong Liu, Rudi Appels, Zhonghu He","doi":"10.1016/j.molp.2025.03.006","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.006","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive omics resource and genetic tools for functional genomics research and genetic improvement of sorghum.","authors":"Chengxuan Chen, Fengyong Ge, Huilong Du, Yuanchang Sun, Yi Sui, Sanyuan Tang, Zhengwei Shen, Xuefeng Li, Huili Zhang, Cuo Mei, Peng Xie, Chao Li, Sen Yang, Huimin Wei, Jiayang Shi, Dan Zhang, Kangxu Zhao, Dekai Yang, Yi Qiao, Zuyong Luo, Li Zhang, Aimal Khan, Baye Wodajo Abey, Yaorong Wu, Ran Xia, Chuanyin Wu, Chengzhi Liang, Qi Xie, Feifei Yu","doi":"10.1016/j.molp.2025.03.005","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.005","url":null,"abstract":"<p><p>Sorghum, the fifth most important food crop globally, serves not only as a source of silage forage, fiber, syrup, and biofuel, but also is widely recognized as an ideal model crop for studying stress biology due to its exceptional ability to tolerate multiple abiotic stresses, including high salt-alkali conditions, drought, and heat. However, conducting functional genomics studies on sorghum has been challenging, primarily due to the limited availability of genetic resources and effective genetic transformation techniques. In this study, we developed a comprehensive and systematic resource platform (https://sorghum.genetics.ac.cn/SGMD) aiming to advance the genetic understanding of sorghum. Our effort encompassed a telomere-to-telomere (T2T) genome assembly of an inbred sorghum line, E048, yielding 729.46 Mb of sequence data representing the complete genome. Alongside the high-quality sequence data, a gene-expression atlas covering 13 distinct tissues was developed. Furthermore, we constructed a saturated ethyl methane sulfonate (EMS) mutant library, comprising 13,226 independent mutants. Causal genes in chlorosis and leafy mutants from the library were easily identified by leveraging the MutMap and MutMap+ methodologies, demonstrating the powerful application of this library for identifying functional genes. To further facilitate the sorghum research community, we performed whole-genome sequencing (WGS) of 179 M₂ mutant lines, resulting a total of 2,291,074 mutations that covered 97.54% of all genes. In addition, an Agrobacterium-mediated sorghum transformation platform was established for gene function studies. In summary, this work established a comprehensive platform, providing valuable resources for functional genomics investigations and genetic improvement of sorghum.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensing H₂O₂ for defense in plants.","authors":"Yan Bi, Leeza Tariq, Hui Wang, Fengming Song","doi":"10.1016/j.molp.2025.03.004","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.004","url":null,"abstract":"<p><strong>Short summary: </strong>Plant cells sense H₂O₂, a major reactive oxygen species (ROS), through cysteine oxidation of critical immune regulators. Recently, Liao et al. reported that the rice transcription factor bHLH25 directly perceives pathogen-induced H₂O₂ via methionine oxidation to trigger defense responses. This study highlights the importance and complexity of H₂O₂-mediated oxidation modification in plant immunity.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}