Molecular Plant最新文献

{"title":"BAI1: A Plant Transcription Factor Governing Nuclear and Plastid Gene Expression.","authors":"Nicolaj Jeran, Simona Masiero, Paolo Pesaresi","doi":"10.1016/j.molp.2025.06.005","DOIUrl":"https://doi.org/10.1016/j.molp.2025.06.005","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorylation-activated G-Protein signaling stabilizes TCP14 and JAZ3 to repress JA signaling and enhance plant immunity.","authors":"Haiyan Jia, Natalie Hewitt, Lucia Jorda, Tigran M Abramyan, Josh Tolliver, Janice L Jones, Kinya Nomura, Jing Yang, Sheng-Yang He, Alexander Tropsha, Antonio Molina, Henrik G Dohlman, Alan M Jones","doi":"10.1016/j.molp.2025.06.004","DOIUrl":"https://doi.org/10.1016/j.molp.2025.06.004","url":null,"abstract":"<p><p>The plant hormones salicylic acid (SA) and jasmonic acid (JA) act in mutual negative-feedback regulation to balance the plant growth-defense trade-off. Heterotrimeric Gα-Gβ-Gγ proteins are hubs that regulate defense signaling. In Arabidopsis, the Gα (GPA1) and Gβ (AGB1) subunits are required for defense against biotrophic and necrotrophic pathogens. We found that G proteins primarily are negative regulators of JA signaling in response to pathogen attack. Both TCP14 and JAZs are transcriptional regulators operating in the JA pathways. Mechanistically, GPA1 interacts with TCP14 within nuclear foci and AGB1 interacts with TCP14 and most of the JAZs regulators including JAZ3. GPA1 slows the proteasomal degradation of the G protein-TCP14-JAZ3 complex, a process that is normally promoted by JA and the bacterial virulence effector HopBB1, thus boosting SA-based defense. GPA1 activity is regulated by JA-induced phosphorylation of a conserved residue located near the nucleotide-binding pocket and other residues within the N-terminal α-helix. These phosphomimic mutants, do not affect GTP binding or hydrolysis, but enhance GPA1 interaction with TCP14 and JAZ3, thereby preventing their degradation. This newly discovered phosphorylation-dependent mechanism of de-sequestering G protein partners to modulate transcriptional regulation may extend to both yeast and human cells.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming Bottlenecks in Virus-Mediated Gene Editing in Plants.","authors":"Lingran Zhang, Vishal, Manman Hu, Degao Liu","doi":"10.1016/j.molp.2025.06.003","DOIUrl":"https://doi.org/10.1016/j.molp.2025.06.003","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA demethylase augments RNA-directed DNA methylation by enhancing CLSY gene expression in maize and Arabidopsis.","authors":"Qiang Xu, Qiuxin Kan, Zhixiang Luo, Qiang Zhang, Liang Dong, Nathan M Springer, Qing Li","doi":"10.1016/j.molp.2025.06.002","DOIUrl":"https://doi.org/10.1016/j.molp.2025.06.002","url":null,"abstract":"<p><p>DNA methylation and demethylation activities are coordinated to maintain DNA methylation patterns. However, the interplay between them remains to be explored. Here, we found loss of DNA demethylase in maize resulted in a significant decrease in CHH methylation, a hallmark of RNA-directed DNA methylation (RdDM). Further analyses suggested that this was caused by the reduced expression in the DNA demethylase mutant of the three CLASSY (CLSY) genes, which encode chromatin remodelers controlling small RNA production. Multiple lines of evidence confirm that the expression of the three maize CLSY genes is sensitive to the DNA methylation levels at their promoter regions, which are regulated by both the DNA demethylase and the RdDM pathway. Moreover, Arabidopsis DNA demethylase mutants also showed reduced CHH methylation, and reduced expression of CLSY1, one of the four CLSY genes in Arabidopsis. Similar to the maize observations, the expression of CLSY1 is associated with promoter DNA methylation levels that are targets of both DNA demethylase and RdDM pathways. These results suggest a conserved interplay between DNA demethylation and RdDM pathways and provide a mechanism to maintain homeostasis of DNA methylation levels across plants.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of the CNGC2-CNGC4 channel complex by P2K1-mediated phosphorylation links extracellular ATP perception to calcium signaling in plant immunity.","authors":"Yujia Sun, Lei Gao, Yiping Han, Changxin Feng, Zebin Liu, Chunyan Li, Yuxin Dong, Shuxin Yin, Laihao Liu, Ming Yang, Qi Niu, Dongdong Kong, Liangyu Liu, Jingbo Zhang, Wang Tian, Sheng Luan, Legong Li, Congcong Hou","doi":"10.1016/j.molp.2025.06.001","DOIUrl":"https://doi.org/10.1016/j.molp.2025.06.001","url":null,"abstract":"<p><p>Extracellular ATP (eATP) functions as a damage-associated molecular pattern (DAMP) in plant immunity. P2K1, a purinergic receptor with a cytoplasmic serine/threonine kinase domain, initiates ATP-responsive signaling cascades that include a rapid spike of cytosolic Ca as a critical second messenger. In this study, we identified cyclic nucleotide-gated channel (CNGC)2-CNGC4 as essential in eATP-induced calcium signaling and bacterial resistance in plants. A biochemical link between eATP perception and CNGC2-CNGC4 was established by revealing the physical association of CNGC channel with eATP receptor P2K1 at the plasma membrane. Our study further demonstrated that P2K1 phosphorylates the CNGC2 subunit of the CNGC2-CNGC4 channel in response to eATP. This phosphorylation-dependent mechanism links eATP perception to calcium signaling, elucidating how P2K1 translates eATP binding into calcium influx. Based on AlphaFold-Multimer predictions, electrophysiology and genetic evidence, we identified residues S705 and S718 in CNGC2 as the phosphorylation sites responsible for P2K1-mediated activation of the channel and for ATP-mediated immunity. Notably, P2K1 selectively phosphorylates CNGC2, in contrast to BIK1's phosphorylation of CNGC4 in pathogen-associated molecular pattern (PAMP)-triggered immunity. Together, our findings suggest that the CNGC2-CNGC4 channel complex serves as a core component in calcium-dependent plant immunity, with different kinases phosphorylating specific subunits in response to distinct immune elicitors.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pan-genome and haplotype map of cassava cultivars and wild ancestors provide insights into its adaptive evolution and domestication.","authors":"Zhiqiang Xia, Zhenglin Du, Xincheng Zhou, Sirong Jiang, Tingting Zhu, Le Wang, Fei Chen, Luiz Carvalho, Meiling Zou, Luis Augusto Becerra Lopez-Lavalle, Xiaofei Zhang, Liangye Xu, Zhenyu Wang, Meili Chen, Xin Guo, Shujuan Wang, Mengtao Li, Yuanchao Li, Haiyan Wang, Shisheng Liu, Yuting Bao, Long Zhao, Chenji Zhang, Jianjia Xiao, Fengguang Guo, Xu Shen, Haozheng Li, Cheng Lu, Fei Qiao, Hernan Ceballos, Huabing Yan, Xiaochun Qin, Ling Ma, Huaifang Zhang, Shuang He, Wenming Zhao, Yinglang Wan, Yinhua Chen, Dongyi Huang, Kaimian Li, Bin Liu, Ming Peng, Weixiong Zhang, Birger Lindberg Møller, Xin Chen, Ming-Cheng Luo, Jingfa Xiao, Wenquan Wang","doi":"10.1016/j.molp.2025.05.014","DOIUrl":"10.1016/j.molp.2025.05.014","url":null,"abstract":"<p><p>Cassava is a highly resilient tropical crop that produces large, starchy storage roots and high biomass. However, how did cassava's remarkable environmental adaptability and key economic traits evolve from its wild species remain unclear. In this study, we obtained near complete telomere-to-telomere genome assemblies and their haplotype forms for the cultivar AM560, the wild ancestors FLA4047 and W14, constructed a graphic pan-genome of 30 representatives with a size of 1.15 Gb, and built a clarified evolutionary tree of 486 accessions. A comparison of structural variations and single-nucleotide variations between the ancestors and cultivated cassavas reveals predominant expansions and contractions of numbers of genes and gene families, which are mainly driven by transposons. Significant selective sweeping occurred in 122 footprints of genomes and affects 1,519 domesticated genes. We identify selective mutations in MeCSK and MeFNR2 that could promote photoreactions associated with MeNADP-ME in C₄ photosynthesis in modern cassava. Coevolution of retard floral primordia and initiation of storage roots may arise from MeCOL5 variants with altered bindings to MeFT1, MeFT2, and MeTFL2. Mutations in MeMATE1 and MeGTR occur in sweet cassava, and MeAHL19 has evolved to regulate the biosynthesis, transport, and endogenous remobilization of cyanogenic glucosides in cassava. These extensive genomic and gene resources provided here, along with the findings on the evolutionary mechanisms responsible for beneficial traits in modern cultivars, lay a strong foundation for future breeding improvements of cassava.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1047-1071"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulated activation of helper NLRs triggers cell death.","authors":"Manman Zhang, Xiwang Zhao, Cheng Li, Huan Chen, Fengquan Liu","doi":"10.1016/j.molp.2025.04.012","DOIUrl":"10.1016/j.molp.2025.04.012","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"928-930"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insects manipulate plant immunity via a cross-kingdom small RNA effector.","authors":"Murad Ghanim","doi":"10.1016/j.molp.2025.04.002","DOIUrl":"10.1016/j.molp.2025.04.002","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"923-924"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STP2-mediated sugar transport in tomato shoot apices is critical for CLV3 arabinosylation and fruit locule development under low temperatures.","authors":"Yimei Li, Jiao Wang, Xiao Liang, Shurong Wu, Jie Zhang, Changqi Wu, Anran Wang, Hanmo Fang, Shuting Ding, Jingquan Yu, Shuang Wu, Huan Liu, Kai Shi","doi":"10.1016/j.molp.2025.05.002","DOIUrl":"10.1016/j.molp.2025.05.002","url":null,"abstract":"<p><p>Prolonged exposure to low temperatures during agricultural production often leads to fruit malformation in crops, significantly reducing market value. However, the underlying molecular mechanisms remain poorly understood. In this study, we identify sugar transport protein 2 (STP2) as a critical regulator of tomato fruit locule development under cold conditions. Low temperatures impair long-distance sucrose transport from leaves to shoot apices, resulting in reduced accumulation of glucose and arabinose. In response, STP2 expression is strongly upregulated in shoot apices, promoting glucose and arabinose transport. We found that the CLAVAT3-WUSCHEL (CLV3-WUS) regulatory module, which governs locule formation, relies on STP2-mediated sugar transport for CLV3 arabinosylation. Overexpression of STP2 promotes glucose and arabinose accumulation in shoot apices, enhances CLV3 arabinosylation and the WUS suppression, mitigating the multi-locular malformations induced by low temperatures. Conversely, disruption of STP2 function exacerbates locule number increases under low temperatures, which could not be rescued by exogenous sugar supplementation. Our findings reveal a key mechanism by which STP2-mediated sugar transport supports CLV3 arabinosylation to maintain fruit locule development under low temperatures, offering potential strategies to alleviate fruit malformations in winter crop cultivation.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1014-1028"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new class of receptors for plant G-proteins.","authors":"Sona Pandey","doi":"10.1016/j.molp.2025.04.011","DOIUrl":"10.1016/j.molp.2025.04.011","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"925-927"},"PeriodicalIF":17.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}