Molecular Plant最新文献

{"title":"When function shapes form: Dynamic control of plant vascular development.","authors":"Thomas Greb","doi":"10.1016/j.molp.2026.07.018","DOIUrl":"10.1016/j.molp.2026.07.018","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1947-1949"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148605516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UFL1-mediated UFMylation stabilizes ABI5 to strengthen ABA-dependent seed dormancy in Arabidopsis.","authors":"Mengrui Wen, Ruihua Huang, Zixuan Ma, Huizi Huang, Jianhai Mo, Yifeng Yang, Minghui Liu, Zhengxi Wu, Yuxi Liao, Yixian Zeng, Miaolun Yuan, Qiuchan Lu, Shengchun Zhang","doi":"10.1016/j.molp.2026.07.016","DOIUrl":"10.1016/j.molp.2026.07.016","url":null,"abstract":"<p><p>UFMylation is a reversible ubiquitin-like modification with an emerging and essential role in mammals, yet its functional mechanisms in plants remain largely unknown. In this study, we systematically characterized the complete UFMylation pathway in Arabidopsis thaliana and demonstrated that its core components-E1 UBA5, E2 UFC1, and E3 UFL1-possessed potent enzymatic activity. UFL1 is an E3 ligase that localizes to both the nucleus and cytoplasm. Expression pattern analyses revealed that UFL1 is strongly upregulated during embryogenesis and progressively accumulates throughout seed maturation. Phenotypic analyses showed that UFL1 overexpression enhances seed dormancy, increases plant sensitivity to abscisic acid (ABA), and significantly delays seed germination and cotyledon greening, identifying UFL1 as a previously unrecognized positive regulator of ABA signaling. Biochemical analyses further demonstrated that UFL1 physically interacts with the bZIP transcription factor ABI5 and mediates its UFMylation, thereby antagonizing ubiquitination and stabilizing ABI5 protein abundance. Genetic evidence further supported that ABI5 acts downstream of UFL1 to regulate seed developmental processes. Collectively, our findings reveal UFMylation as a pivotal regulatory layer in plants and uncover a UFL1-ABI5 signaling module that coordinates seed maturation, dormancy, and germination.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"2031-2052"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strigolactones restrict sugar acquisition in rice tiller buds to inhibit their outgrowth by suppressing the expression and polarized localization of two monosaccharide transporters.","authors":"Tianhao Wang, Tianyu Mo, Yinglu Sun, Miao Miao, Yan Chun, Ashmit Kumar, Jinfeng Zhao, Xueyong Li","doi":"10.1016/j.molp.2026.08.003","DOIUrl":"10.1016/j.molp.2026.08.003","url":null,"abstract":"<p><p>Strigolactones (SLs) are fundamental phytohormones that suppress tillering, whereas sugars act as both energy sources and signaling molecules to promote tillering. However, the molecular interplay between SLs and sugars remains largely underexplored. In this study, through isobaric tags for relative and absolute quantification-based quantitative proteomic analysis, we identified two highly homologous monosaccharide transporters, OsMST3 and OsMST6, showing increased protein abundance in the SL signaling mutant d3^s2-215 and biosynthesis mutant d10^XJ1705. Using feeding assays with fluorescence- or isotope-labeled sugars, we showed that OsMST3 and OsMST6 function as influx transporters of glucose and fructose, facilitating their translocation from leaf blades to tiller buds to promote bud outgrowth. Interestingly, glucose and fructose not only induce OsMST3 and OsMST6 expression but also promote polarized plasma membrane localization of their encoded proteins, both essential for bud outgrowth, whereas SLs suppress their expression and counteract sugar-induced polarized localization of OsMST3 and OsMST6. Furthermore, we revealed that OsTB1, a key transcription factor downstream of SL signaling, directly binds the OsMST3 and OsMST6 promoters to inhibit their transcription. Tiller bud outgrowth of the tb1^SG0312 mutant is severely inhibited by a non-metabolizable glucose analog (2-deoxy-D-glucose) and an inhibitor of sugar transporters (carbonyl cyanide m-chlorophenylhydrazone). Moreover, simultaneous knockout of OsMST3 and OsMST6 could rescue high-tillering phenotype of the d3^s2-215, d10^XJ1705, and tb1^SG0312 mutants. Collectively, our study reveals a novel mechanism by which SLs control tillering by limiting sugar allocation to buds through suppression of the monosaccharide transporters OsMST3 and OsMST6.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"2093-2121"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rethinking nitrogen-use efficiency in maize: Moving forward.","authors":"Bertrand Hirel","doi":"10.1016/j.molp.2026.07.021","DOIUrl":"10.1016/j.molp.2026.07.021","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1944-1946"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148605522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The origins and evolution of the RNA virome in land flora.","authors":"Qiyan Liu, Zhen Gong, Eugene V Koonin, Valerian V Dolja, Guan-Zhu Han","doi":"10.1016/j.molp.2026.07.023","DOIUrl":"10.1016/j.molp.2026.07.023","url":null,"abstract":"<p><p>The colonization of land by plants was a major transition in the history of life, fundamentally reshaping the evolution of terrestrial organisms and Earth's ecosystems. Plants are hosts to an enormous diversity of viruses, but the origins and evolution of these viruses are not thoroughly understood. In this study, we mined the transcriptomes and genomes of 2402 species sampled across the plant kingdom and discovered about 35 000 plant-associated RNA viruses, including several potential families not described previously, resulting in a 2.2-fold increase in the known plant-associated RNA virus diversity, greatly expanding the virus host ranges, and revealing many groups of atypical, apparently persistent viruses. Phylogenetic analysis shows that plant viruses emerged on numerous independent occasions, mostly via horizontal virus transfer facilitated by close ecological associations of plants with other terrestrial organisms, such as invertebrates and fungi. Collectively, our work substantially expands the known diversity of plant RNA viruses, provides a major resource for studying plant RNA virome, and contributes to understanding the origins and natural history of plant RNA viruses.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"2053-2074"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13501481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148605558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The sugar-sensing kinase OSK3-mediated destabilization of OsbZIP58 promotes pre-harvest sprouting in rice under high temperature.","authors":"Heng Xu, Yuhong Tan, Fudeng Huang, Zhiping Deng, Ningning Zhang, Meihao Sun, Hua Zhang, Heng Zhang, Tao Song, Fulin Wang, Yu Han, Junmin Wang, Sheng Teng, García-Caparrós Pedro, Xiangyang Hu, Ying Zhu","doi":"10.1016/j.molp.2026.07.012","DOIUrl":"10.1016/j.molp.2026.07.012","url":null,"abstract":"<p><p>Pre-harvest sprouting (PHS) is a major problem in cereal production, severely reducing yield and grain quality, particularly under high-temperature (HT) and excessive-rainfall conditions during grain filling. However, the molecular mechanisms by which HT triggers seed dormancy breakdown and promotes PHS remain largely unknown. In this study, we reveal that the rice transcription factor OsbZIP58, previously implicated in seed storage reserve accumulation, integrates HT-induced sugar depletion into precocious seed dormancy release. By combining DNA affinity purification sequencing, chromatin immunoprecipitation-qPCR, and genetic epistasis analyses, we show that OsbZIP58 directly activates the abscisic acid-responsive gene RAB16A while repressing the α-amylase gene OsAmy3D, thereby maintaining seed dormancy under normal conditions. Meanwhile, the energy-sensing sucrose non-fermenting-1-related protein kinase 1-family kinase OSK3, a direct upstream regulator, phosphorylates OsbZIP58 at Ser46 to promote its proteasomal degradation, consequently derepressing OsAmy3D expression. Mechanistically, HT accelerates grain filling and causes premature sugar depletion, which activates OSK3 to trigger OsbZIP58 degradation, thereby releasing OsAmy3D activity to increase starch hydrolysis and soluble sugar levels in seeds, ultimately leading to PHS. Elevated soluble sugar levels restrain OSK3 hyperactivation under HT through a negative-feedback mechanism. Furthermore, haplotype analysis reveals that natural variation in the OsbZIP58 locus is associated with variation in PHS susceptibility among rice accessions. Collectively, our work uncovers the OSK3-OsbZIP58-OsAmy3D module in regulating PHS in response to HT and establishes a previously unrecognized signaling cascade that integrates cellular energy status with developmental timing and thermal stress, providing genetic targets for breeding climate-resilient PHS-resistant cereals.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"2010-2030"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148456410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure-aware prediction of functional non-synonymous variants in a maize EMS mutant library.","authors":"Li Qin, Xiaolong Guo, Fan Xia, Fugui Xie, Yu Bao, Manzhu Jiang, Tianhao Wu, Yuxuan Lou, Xiangfeng Wang, Xuerong Yang, Xiaoduo Lu","doi":"10.1016/j.molp.2026.07.024","DOIUrl":"10.1016/j.molp.2026.07.024","url":null,"abstract":"<p><p>Ethyl methanesulfonate (EMS) mutagenesis is widely used to generate genetic variation for gene-function studies. Previous studies have traditionally focused on stop-gained mutations, because these variants usually disrupt protein-coding capacity and produce detectable phenotypes. Thus, non-synonymous mutations with more moderate effects are often overlooked, despite their potential utility for fine-tuning traits in molecular breeding. Here, we present an updated maize EMS mutation resource, maizeEMSDBv2 (https://maizeems.qlnu.edu.cn), generated by whole-genome and whole-exome sequencing datasets of 8556 EMS-mutagenized B73-derived inbred lines. Among 20.23 million GC-to-AT mutations identified in this high gene-level coverage resource, 4,240,616 were located within genic regions, covering 96.34% of annotated genes across the maize genome. To enable systematic evaluation of 1,045,505 missense mutations, we developed the Maize Missense Effect Prediction platform, MaizeMEP (https://maizemeps.qlnu.edu.cn), which integrates protein language model-based, structure-aware predictions of mutational effects (MEs) and biomolecular binding properties using SaProt and GPSite. Genome-wide analysis revealed that 20.8% and 15.4% of non-synonymous mutations showed moderate and high negative MEs, respectively, whereas 4.17% and 0.40% showed moderate and high positive MEs, respectively. To demonstrate the utility of MaizeMEP and associated tools, we examined three representative genes previously shown to regulate plant height in maize: Brachytic2 (ZmBR2), involved in auxin polar transport, and Kaurene synthase 3 (ZmKS3) and Anther ear 1 (ZmAN1), both involved in gibberellin biosynthesis. We identified three ZmBR2 missense variants, G419R, G636D, and S535F, that significantly reduced plant height while having limited impact on grain yield, supporting the feasibility of exploiting EMS-induced single amino acid substitutions for trait fine-tuning in maize molecular breeding.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"2075-2092"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective recruitment of NAT10-catalyzed ac⁴C-modified mRNAs into stress granules by poly(A)-binding protein promotes mRNA stability and plant heat-stress tolerance.","authors":"Wanhong Zhang, Yanxiao Bu, Yubing Jiao, Yingwen Wang, Lili Shen, Ying Li, Binna Lv, Tzu-Chieh Ho, Yih-Cherng Liou, Chin-Kuo Chen, Fei Han, Robert Y L Wang, Jinguang Yang","doi":"10.1016/j.molp.2026.08.004","DOIUrl":"10.1016/j.molp.2026.08.004","url":null,"abstract":"<p><p>Global warming poses a considerable threat to crop production, making heat stress a challenge in agriculture, yet how epitranscriptomic modifications contribute to plant heat-stress responses remains to be explored. This study reveals the role of phase separation in plant heat-stress tolerance and shows that N-acetyltransferase 10 (NAT10), which encodes a cytosine N4 acetyltransferase, contributes to heat resistance. We found that NAT10 interacts with polyadenylate-binding protein (PABP), which contains intrinsically disordered regions, thereby facilitating the selective recruitment of ac⁴C-modified mRNAs into PABP-mediated condensates. Integrative transcriptome-wide analysis, combining ac⁴C acetylome profiling with stress-granule-enriched transcript sequencing, revealed that detoxification-related mRNAs, including those encoding the cytochrome P450, phenylalanine ammonia-lyase, glutathione S-transferase, and heat shock 70 protein families, preferentially accumulate within these condensates. This accumulation maintains their stability and prevents stress-induced degradation. Conversely, loss of PABP function impairs the recruitment of ac⁴C-modified detoxification-related transcripts into stress granules, thereby promoting their degradation under heat stress. In summary, our findings identify a stress-responsive NAT10-PABP-ac⁴C axis that promotes phase separation to stabilize ac⁴C-modified mRNAs under heat stress. By recruiting detoxification-related transcripts into stress granules, this axis ensures mRNA stability under environmental stress, offering new insights for enhancing crop stress resilience.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"2122-2139"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148707372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensing pectin damage: Rhamnogalacturonan-I as a ligand for receptor kinases and an immune elicitor.","authors":"Jana Ordon","doi":"10.1016/j.molp.2026.07.022","DOIUrl":"10.1016/j.molp.2026.07.022","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1950-1952"},"PeriodicalIF":32.7,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-nucleus transcriptome atlas of Brassica developing seeds provides cellular and molecular insights into embryo abortion in interspecific hybrids","authors":"Qinjie Chu,Xiaojing Zhang,Guangqi Zhu,Renyi Zhang,Shuang He,Kexin Liang,Xiaohong Zhao,Rana Muhammad Amir Gulzar,Muhammad Haseeb Javaid,Bingjie Xu,Shihui Pan,Jiayun Yang,Nuo Chen,Sue Lin,Xiaofeng Li,Pengpeng Zhang,Yi He,Qiaohong Duan,Jiashu Cao,Li Huang,Jiabao Huang","doi":"10.1016/j.molp.2026.09.016","DOIUrl":"https://doi.org/10.1016/j.molp.2026.09.016","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"269 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149090451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}