Molecular Plant最新文献

{"title":"The hypoxic niche enclosing the shoot apical meristem is shaped by a combination of morphological features and metabolic activity.","authors":"Viktoriia Voloboeva, Bart Dequeker, Leen Van Doorselaer, Gabriele Panicucci, Pierdomenico Perata, Pieter Verboven, Bart Nicolai, Daan A Weits","doi":"10.1016/j.molp.2026.02.011","DOIUrl":"10.1016/j.molp.2026.02.011","url":null,"abstract":"<p><p>Stem cell niches in both plants and animals are frequently located in low-oxygen microenvironments that support their function. In plants, these hypoxic niches promote local stabilization of several transcriptional regulators that control a range of developmental processes, including shoot apical meristem (SAM) activity, vernalization, lateral root development, and leaf growth and morphogenesis. Despite their importance, however, it remained unclear how these hypoxic niches are maintained. In this study, we employed a combination of experimental and modeling approaches to identify the key features required to establish and sustain the hypoxic niche enclosing the SAM. Using respiration inhibitors, manipulation of resource availability, and mitochondria mutant lines, we found that respiratory oxygen consumption is required to establish the hypoxic niche. Oxygen microprofiling and imaging of hypoxia signaling in cuticle biosynthesis mutants, as well as following targeted cuticle degradation, revealed that a cuticle-like barrier defines the steepness of the oxygen gradient and ensures that even the outermost layer remains hypoxic. Moreover, high tissue compactness in the shoot apex region was visualized using X-ray micro-computed tomography and shown to stabilize the hypoxic microenvironment by limiting internal oxygen diffusion. Finally, sensitivity tests on a novel reaction-diffusion model closely recapitulated oxygen gradients across the SAM and revealed distinct roles of each feature and their combined effect on oxygen distribution. Together, these findings explain how the SAM sustains hypoxia and point to a potential universal strategy used by stem cell niches to maintain low oxygen levels.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1080-1099"},"PeriodicalIF":24.1,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A redox-driven phase switch: RCD1 condensates decode growth and stress adaptation.","authors":"Seol Ki Paeng, Ho Byoung Chae, Su Bin Bae, Seong Dong Wi, Sang Yeol Lee","doi":"10.1016/j.molp.2026.03.003","DOIUrl":"10.1016/j.molp.2026.03.003","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"943-945"},"PeriodicalIF":24.1,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147369803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatially compartmentalized dual-epitope engineering: A strategy for multilayered plant defense.","authors":"Xiumei Luo, Ramesh Raju Vetukuri, Kerimaliev Janybek, Zheng Qing Fu, Maozhi Ren","doi":"10.1016/j.molp.2026.02.012","DOIUrl":"10.1016/j.molp.2026.02.012","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"940-942"},"PeriodicalIF":24.1,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147308084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An NLR-TF immune module under asymmetric selection shapes rice immunity and yield.","authors":"Cailin Hu, Yuese Ning, Wei Li, Guo-Liang Wang","doi":"10.1016/j.molp.2026.04.013","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.013","url":null,"abstract":"<p><p>Artificial selection of functional genes shapes crops agronomic traits including disease resistance and reproductive development. Recently, Lin et al. (2026) reported that rice nucleotide-binding leucine-rich repeat receptor (NLR) XA48 and its associated transcription factors (TFs) OsVOZ1/2 underwent differential artificial selection upon infection by diverse Xanthomonas oryzae pv. oryzae (Xoo) strains. This asymmetric selection has shaped rice resistance to Xoo and grain yield, uncovering a novel NLR-TF immune module (XA48-VOZs). Here, we discuss the molecular mechanisms of this module, the evolutionary implication of its differential selection, and its potential application, particularly in combination with the pattern-recognition receptor (PRR) XA21 for breeding rice varieties with broad-spectrum resistance to bacterial leaf blight (BLB).</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147817843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of pennogenin synthases in Paris polyphylla reveals enzyme plasticity underlying paralog evolution","authors":"Shuyu Li, Jingjing Liao, Jingjing Zhang, Chunjin Fu, Haining Lyu, Yuxiang Huang, Zhangrui Tang, Xinwei Zhang, Yuyang Liu, Xin Chai, Jiale Xing, Xin Zhao, Qian Zhang, Huanling Yang, Qing Zhao, Jigang Wang, Jiaqi Wang, Chengchao Xu","doi":"10.1016/j.molp.2026.04.011","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.011","url":null,"abstract":"While artificially generated mutants offer valuable insights into protein evolutionary trajectories in laboratory settings, plasticity in paralog evolution during natural evolutionary processes remains poorly explored. Here, we identified <ce:italic>Pp</ce:italic>CYP81BY1 as the missing pennogenin synthases in <ce:italic>Paris polyphylla</ce:italic> and completed the heterologous reconstitution of multiple pharmaceutically active paris saponins in <ce:italic>Nicotiana benthamiana</ce:italic>. The serendipitous discovery of multiple highly-similar paralogs of <ce:italic>Pp</ce:italic>CYP81BY1 suggests recent gene duplication events, providing an interesting case to investigate the functional fates of enzymes following gene duplication in plant metabolic evolution. Structural-guided modelling and functional analyses revealed the divergent fates of these highly-similar paralogs: whereby a single nucleotide substitution in one paralog abolishes synthase activity, while a secondary mutation in another paralog restores the activity through epistatic effects. Thus, the functional fate of paralogous enzymes in natural evolution can be switched through minimal genetic changes. Given that pennogenin synthase has also been identified in <ce:italic>Ypsilandra thibetica</ce:italic>, belonging to a genus that diverged from <ce:italic>Paris</ce:italic> prior to the massive genome expansions that arose in <ce:italic>Paris</ce:italic> species, their common ancestor likely encoded this enzyme during the evolution of <ce:italic>Melanthiaceae</ce:italic>. Taken together, our findings likely capture the relatively early-stage evolution of paralogs in <ce:italic>P. polyphylla</ce:italic>. The functional fate-switching could be explained by a single nucleotide substitution, which preceded subsequent mutations that may obscure a mechanistic understanding of these changes, thereby suggestive of possible evolutionary reversibility. Combined with previous findings, we propose a framework for understanding paralog evolution across life.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"152 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new oxidative pathway of nitric oxide production from oximes in plants","authors":"Pedro López-Gómez, Javier Buezo, Marina Urra, Alfonso Cornejo, Raquel Esteban, Jorge Fernández de los Reyes, Estibaliz Urarte, Estefanía Rodríguez-Dobreva, Alejandro Chamizo-Ampudia, Alejandro Eguaras, Sebastian Wolf, Daniel Marino, Victor Martínez-Merino, Jose F. Moran","doi":"10.1016/j.molp.2026.04.005","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.005","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"9 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Receptor-like protein kinases in plants: Post-translational regulation and functional effects","authors":"Yuxiu Li, Xiaodong Wang, Dongping Lu, Xiaoping Gou, Zhuoyun Wei","doi":"10.1016/j.molp.2026.04.012","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.012","url":null,"abstract":"Receptor-like protein kinases (RLKs) serve as critical regulators in diverse biological processes, ranging from modulation of growth and development to responses to complicated environmental stimuli. These proteins typically mediate cellular signaling through diverse post-translational modifications (PTMs), spanning reversible phosphorylation and dephosphorylation events, ubiquitination and deubiquitination dynamics, N-glycosylation, lipid anchoring via N-myristoylation and S-acylation, SUMOylation and deSUMOylation, and pathogen effector-mediated acetylation and uridylylation. Emerging evidence demonstrates that multiple RLKs undergo proteolytic cleavage to generate truncated intracellular kinase domains that translocate to specific subcellular compartments, where they execute non-canonical signaling functions. This review summarizes two pivotal regulatory mechanisms: 1) dynamic PTM-mediated control of RLK activation states and signaling outputs; and 2) proteolytic cleavage-dependent generation of mobile kinase modules that spatially and temporally expand the functions of RLKs. Unraveling the sophisticated post-translational regulatory mechanisms of RLKs will remain an important direction and challenge for future research, which will facilitate the genetic improvement of crops through targeted manipulation of these mechanisms.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"21 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compelling evidence supporting that cassava is a C3 plant","authors":"Mark Stitt, Wolfang Zierer, Stephanie Arrivault, Uwe Sonnewald, Alisdair R. Fernie","doi":"10.1016/j.molp.2026.04.009","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.009","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"2 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reply to Stitt et al.: Revisiting cassava photosynthesis-Evidence for C4-facultative traits in a C3 plant","authors":"Haozheng Li, Shujuan Wang, Xianhai Xie, Xu Shen, Haiyan Wang, Xincheng Zhou, Liangye Xu, Zhiqiang Xia, Jingfa Xiao, Mabrouk El-Sharkawy, Fei Chen, Wenquan Wang","doi":"10.1016/j.molp.2026.04.010","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.010","url":null,"abstract":"This study provides evidences of reduced photorespiration, enhanced activity of the phosphoenolpyruvate carboxylase (PEPC) enzyme, and the presence of chloroplasts within bundle sheath (BS) cells in cassava. It also highlights the evolutionary biological characteristics of cassava in adapting to high light, elevated temperature and increased CO2 concentrations. Based on it, we propose that cassava is considered a C3 plant with C4-facultative traits.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"9 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147752940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordinated action of BSMT1, SABP3, and MAT2 activates methyl salicylate-mediated systemic immunity in plants.","authors":"Ravi Gupta","doi":"10.1016/j.molp.2026.04.008","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.008","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147717387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}