Molecular Plant最新文献_第3页

Host-adapted enzymatic deconstruction of acetylated xylan limits immune activation and facilitates mutualistic colonization of monocot roots. 宿主适应乙酰化木聚糖的酶解限制了免疫激活，促进了单子叶根的共生定植。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2026-04-16 DOI: 10.1016/j.molp.2026.04.006

Mathias Brands, Vicente Ramírez, Laura Armbruster, Ruben Eichfeld, Asmamaw Bidru Endeshaw, Taim Nassr, Pia Saake, Markus Pauly, Alga Zuccaro

{"title":"Host-adapted enzymatic deconstruction of acetylated xylan limits immune activation and facilitates mutualistic colonization of monocot roots.","authors":"Mathias Brands, Vicente Ramírez, Laura Armbruster, Ruben Eichfeld, Asmamaw Bidru Endeshaw, Taim Nassr, Pia Saake, Markus Pauly, Alga Zuccaro","doi":"10.1016/j.molp.2026.04.006","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.006","url":null,"abstract":"Intracellular accommodation of beneficial fungi requires host cell wall remodeling that avoids excessive immune activation. The root endophyte Serendipita indica, a generalist mutualist capable of colonizing both monocot and dicot plants, employs a monocot-specific enzymatic module to deconstruct acetyl-xylan, the dominant hemicellulose of grasses. Central to this module are the glycoside hydrolase SiGH11, which releases acetylated xylooligosaccharides, and SiAXE, a previously uncharacterized SGNH-like acetyl-xylan esterase that sequentially removes acetyl groups from soluble XOS. Both enzymes are co-expressed within a monocot-enriched transcriptional program that also includes sugar transporters and metabolic regulators. Their coordinated activity, together with co-expressed exo-enzymes, promotes efficient xylan hydrolysis while limiting the prolonged accumulation of immunogenic damage-associated molecular patterns (DAMPs). Functional genetics demonstrated that SiAXE is required for sustained intracellular growth in monocot roots: its deletion impaired colonization, whereas overexpression transiently accelerated entry but provoked immune responses, underscoring the importance of temporal regulation and enzyme coordination for immune-compatible colonization. These findings provide mechanistic insight into an immune-compatible fungal strategy for host cell wall remodeling and reveal how a broadly colonizing mutualist has repurposed ancestral saprotrophic enzymes into specialized host-adapted modules that balance nutrient acquisition with immune modulation.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147717410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergizing Genome Editing and Artificial Intelligence for Predictive Crop Design. 协同基因组编辑和人工智能用于预测作物设计。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2026-04-16 DOI: 10.1016/j.molp.2026.04.007

Zhaoxu Gao, Jinjie Zhu, Chuanxiao Xie

{"title":"Synergizing Genome Editing and Artificial Intelligence for Predictive Crop Design.","authors":"Zhaoxu Gao, Jinjie Zhu, Chuanxiao Xie","doi":"10.1016/j.molp.2026.04.007","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.007","url":null,"abstract":"The convergence of genome editing (GE) and artificial intelligence (AI) is shifting crop improvement from empirical optimization toward predictive design. In this Perspective, we propose that GE and AI are linked by a reciprocal innovation cycle. AI improves GE by enabling more accurate guide RNA design, editing outcome and off-target prediction, and data-driven development of next-generation editing systems. In turn, GE provides a powerful experimental platform to validate AI predictions, dissect causal genotype-to-phenotype relationships, and generate high-resolution perturbation datasets for iterative model refinement. This bidirectional interplay is beginning to accelerate the engineering of complex agronomic traits, including trait stacking, metabolic rewiring, climate resilience, stress tolerance, nutritional enhancement, and de novo domestication. Here, we argue that this synergy is emerging most clearly in predictive editing design and iterative model validation, although robust breeding-scale evidence remains limited. We also discuss emerging opportunities for AI-guided closed-loop editing platforms and generative biological design, together with key bottlenecks, including limited training data, biological complexity across scales, model interpretability, and heterogeneous regulatory landscapes. We argue that integrating AI with GE can establish a practical framework for predictive crop design and enable more efficient and sustainable crop engineering.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147717347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-hydroxypipecolic acid acts as a mobile signal for systemic acquired resistance n -羟基果酸作为全身获得性耐药的移动信号

IF 27.5 1区生物学

Molecular Plant Pub Date : 2026-04-14 DOI: 10.1016/j.molp.2026.04.003

Pingyu Zhang, Danyi Huang, Hainan Tian, Yuelin Zhang

引用次数: 0

A conserved translational repression module buffers sugar-inducible root vascular proliferation 一个保守的翻译抑制模块缓冲糖诱导的根维管增殖

IF 27.5 1区生物学

Molecular Plant Pub Date : 2026-04-14 DOI: 10.1016/j.molp.2026.04.004

Sangkyu Choi, Hyun Seob Cho, Seungchul Lee, Hoyoung Nam, Garam Lee, Heejae Nam, Sion Bae, Ildoo Hwang

{"title":"A conserved translational repression module buffers sugar-inducible root vascular proliferation","authors":"Sangkyu Choi, Hyun Seob Cho, Seungchul Lee, Hoyoung Nam, Garam Lee, Heejae Nam, Sion Bae, Ildoo Hwang","doi":"10.1016/j.molp.2026.04.004","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.004","url":null,"abstract":"Balancing vascular proliferation with sugar availability is essential for optimal carbon allocation; however, the molecular signaling networks integrating sugar status into vascular development remains unclear. Here, using histological, genetic, and pharmacological analyses in the <ce:italic>Arabidopsis</ce:italic> root, we demonstrate that sugar acts as an instructive signal to drive vascular proliferation. Sugar primes cell proliferation and the gene regulatory networks required for vascular development through the TARGET OF RAPAMYCIN (TOR) pathway. We further uncover that this sugar-driven vascular program incorporates a translational repression module comprising the RNA-binding protein JULGI1 (JUL1) and its target, <ce:italic>CLAVATA3/ESR-RELATED41</ce:italic> (<ce:italic>CLE41</ce:italic>) mRNA. This regulation is mediated by a conserved guanine-rich motif in the <ce:italic>CLE41</ce:italic> 5′ UTR, which enables JUL1 binding and imposes a negative feedback regulation on vascular proliferation. Furthermore, phylogenetic and cross-species analyses indicate that the JUL1–<ce:italic>CLE41</ce:italic> module is conserved across cambium-bearing plant lineages. Collectively, these findings establish that sugars directly activate vascular growth, while the JUL1–<ce:italic>CLE41</ce:italic> module constrains this response to ensure balanced proliferation under sugar-rich conditions. Our work provides a framework for viewing vascular proliferation as a sugar-responsive developmental process buffered by intrinsic regulatory mechanisms to maintain homeostasis, a principle that may underlie the evolutionary refinement of sugar-responsive vascular growth strategies.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"39 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147681169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SSBP: A meta-GWAS-based pleiotropy-aware QTL atlas and smart breeding platform for soybean. 基于meta- gwas的大豆多效性感知QTL图谱及智能育种平台。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2026-04-09 DOI: 10.1016/j.molp.2026.04.001

Jinding Liu, Xuteng Ye, Jun Cheng, Lingzhao Fang, Wen Huang, Yihua Zhu, Xing Wang, Daolong Dou

引用次数: 0

From Green Revolution to Multigene Revolution: Breeding High-Yield Rice by Design. 从绿色革命到多基因革命：设计培育高产水稻。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2026-04-08 DOI: 10.1016/j.molp.2026.04.002

Bo Zhang, Qingli Wen, Wenrui Feng, Rong Wang, Wenbo Liu, Wen Huang, Ahmed Khatab, Jiayang Li, Yongzhong Xing

{"title":"From Green Revolution to Multigene Revolution: Breeding High-Yield Rice by Design.","authors":"Bo Zhang, Qingli Wen, Wenrui Feng, Rong Wang, Wenbo Liu, Wen Huang, Ahmed Khatab, Jiayang Li, Yongzhong Xing","doi":"10.1016/j.molp.2026.04.002","DOIUrl":"https://doi.org/10.1016/j.molp.2026.04.002","url":null,"abstract":"Rice has made a significant contribution to global food security over the past half-century. However, the continuous increase in grain yield potential per unit area remains a critical challenge. Recent advances in functional genomics have provided unprecedented opportunities to overcome current barriers to yield enhancement. This review briefly introduces current high-yielding rice varieties by analyzing their key characteristics across the six major rice cropping zones in China. It primarily focuses on the breeding value of major yield-related genes and their intricate regulatory networks by outlining designs to optimize heading date, ideal plant architecture, and photosynthetic efficiency for source enhancement; to coordinate tiller number, panicle structure, and grain shape for sink expansion; to improve the smooth flow of assimilates for optimal yield; and to increase nitrogen and phosphorus utilization efficiency to boost biomass. A breeding design is developed to create interspecific hybrids by producing superior wide compatible lines, which increase hybrid seed production by synchronizing xian-geng parental diurnal floret opening times. Furthermore, we propose a strategy that integrates major-gene-based breeding design with recurrent selection to fully utilize minor genes, thereby enhancing breeding efficiency and effectively breaking through the barriers to high-yield breeding. Ultimately, artificial intelligence-driven prediction and discovery of cis-regulatory elements will facilitate the development of advanced, stress-resilient smart varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytocytokines as regulators of disease resistance in monocot crops. 植物细胞因子在单子叶作物抗病性中的调节作用。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2026-04-06 Epub Date: 2026-01-20 DOI: 10.1016/j.molp.2026.01.004

Vikram Jha, Patricia Zecua-Ramirez, Martin Stegmann

引用次数: 0

Functional divergence of two ethylene receptor subfamilies in calcium permeability. 两个乙烯受体亚家族在钙通透性中的功能分化。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2026-04-06 Epub Date: 2026-03-11 DOI: 10.1016/j.molp.2026.03.007

Chenliang Pan, Junyuan Cheng, Zining Lin, Dongdong Hao, Zhina Xiao, Yuhang Ming, Peng You, Hongtao Yu, Wei Yan, Wen Song, Li Liu, Hongwei Guo

{"title":"Functional divergence of two ethylene receptor subfamilies in calcium permeability.","authors":"Chenliang Pan, Junyuan Cheng, Zining Lin, Dongdong Hao, Zhina Xiao, Yuhang Ming, Peng You, Hongtao Yu, Wei Yan, Wen Song, Li Liu, Hongwei Guo","doi":"10.1016/j.molp.2026.03.007","DOIUrl":"10.1016/j.molp.2026.03.007","url":null,"abstract":"Ethylene is a gaseous plant hormone that regulates plant growth, development, and stress adaptation, yet the molecular mechanism by which ethylene receptors perceive the hormone and initiate downstream signaling remains poorly understood. In this study, using genetic analyses in Arabidopsis thaliana, we reveal that the two ethylene receptor subfamilies do not act in parallel: subfamily I receptors constitute the core ethylene-sensing module, whereas subfamily II receptors require subfamily I receptors to function. Moreover, we show that subfamily I ethylene receptors are required for the rapid phase I growth inhibition that occurs within minutes of ethylene exposure, suggesting the involvement of a fast signaling mechanism. Using electrophysiological assays in Xenopus oocytes and mammalian human HEK293F cells, we reveal that only subfamily I ethylene receptors exhibit Ca2+ permeability. The N-terminal residues of the subfamily I receptor ETHYLENE RESPONSE 1 (ETR1), including Cys65 and Phe76, are essential for this Ca2+ permeability. Furthermore, ethylene promotes ETR1 Ca2+ permeability in the Xenopus oocyte system and induces cytosolic Ca2+ influx in plants in a manner dependent on subfamily I ethylene receptors. Collectively, our work supports a mechanistic framework in which subfamily I ethylene receptors integrate ethylene sensing with calcium influx, providing new insight into how plants translate hormonal cues into downstream signaling events.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"907-919"},"PeriodicalIF":24.1,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of seed germination by a temperature-sensitive feedback mechanism that integrates environmental and hormonal signals. 通过整合环境和激素信号的温度敏感反馈机制调节种子萌发。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2026-04-06 Epub Date: 2026-03-20 DOI: 10.1016/j.molp.2026.03.009

Pannaga Krishnamurthy, Prakash P Kumar

引用次数: 0

Subfamily I ethylene receptors are functionally conserved in calcium permeability across the green lineage. I亚家族乙烯受体在整个绿色谱系中的钙通透性功能上是保守的。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2026-04-06 Epub Date: 2025-12-22 DOI: 10.1016/j.molp.2025.12.021

Dongsheng Yu, Chuanli Ju, Zebin Liu, Changxin Feng, Yu Wang, Yujia Sun, Lei Gao, Chunyan Li, Enjie Yu, Xuan He, Haimei Su, Mengchen Hu, Yidong Wang, Jiayi Liu, Jie Meng, Shen Tian, Liangyu Liu, Congcong Hou, Dongdong Kong, Legong Li

{"title":"Subfamily I ethylene receptors are functionally conserved in calcium permeability across the green lineage.","authors":"Dongsheng Yu, Chuanli Ju, Zebin Liu, Changxin Feng, Yu Wang, Yujia Sun, Lei Gao, Chunyan Li, Enjie Yu, Xuan He, Haimei Su, Mengchen Hu, Yidong Wang, Jiayi Liu, Jie Meng, Shen Tian, Liangyu Liu, Congcong Hou, Dongdong Kong, Legong Li","doi":"10.1016/j.molp.2025.12.021","DOIUrl":"10.1016/j.molp.2025.12.021","url":null,"abstract":"The gaseous hormone ethylene plays a key role in regulating plant growth and stress responses. Although Ca2+ has long been implicated in ethylene signaling, the identity of molecules controlling Ca2+ permeability has remained elusive. In this study, we revealed that Arabidopsis subfamily I ethylene receptors ETR1 and ERS1, as well as their homologs across the green lineage, are Ca2+ permeable. We found that simultaneous disruption of ETR1 and ERS1 markedly attenuates ethylene-induced elevation in cytosolic Ca2+ concentrations in Arabidopsis seedlings, and that both ETR1 and ERS1 exhibit Ca2+ permeability in the Xenopus laevis oocyte system and two additional heterologous expression systems. Moreover, we showed that homologs of ETR1 from eight land plants and algal species also exhibit Ca2+ permeability, suggesting an evolutionarily conserved function. We further demonstrated that ethylene enhances the Ca2+ permeability of ETR1 and its homolog from the charophyte Klebsormidium flaccidum, and a mutation disrupting ethylene binding (Cys65Ser) abolishes the effect of ethylene. These findings uncover a previously unrecognized yet conserved role of ethylene receptors as Ca2+-permeable channels in the green lineage, with broad implications for Ca2+ signaling in plant development and environmental adaptation.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"755-766"},"PeriodicalIF":24.1,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145820314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0