Molecular Plant最新文献_第3页

Design of effective synbiotics against aboveground insect herbivory through characterization of host plant rhizosphere microbiota and metabolites. 通过对寄主植物根际微生物群和代谢物的分析，设计有效的抗地上食草昆虫合生剂。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-29 DOI: 10.1016/j.molp.2025.09.016

Shengdie Yang, T Martijn Bezemer, Xiaohang Yuan, Xiaoyu Liu, Ting Wan, Feihong Liu, Tao Wen, Qirong Shen, Jun Yuan

{"title":"Design of effective synbiotics against aboveground insect herbivory through characterization of host plant rhizosphere microbiota and metabolites.","authors":"Shengdie Yang, T Martijn Bezemer, Xiaohang Yuan, Xiaoyu Liu, Ting Wan, Feihong Liu, Tao Wen, Qirong Shen, Jun Yuan","doi":"10.1016/j.molp.2025.09.016","DOIUrl":"https://doi.org/10.1016/j.molp.2025.09.016","url":null,"abstract":"Plants can cope with stresses via the so-called \"cry for help\" strategy, but how aboveground insect herbivores induce alterations in the rhizosphere microbiota through eliciting this plant-driven response remains unexplored. Here, we exposed cabbage plants to aboveground insect herbivory for five sequential planting rounds in the same soil. New cabbage plants, growing in the soils conditioned for five rounds showed a significant increase in resistance to aboveground insect herbivory. This effect was attributed to the accumulation of Pseudomonas in herbivore-conditioned soils. Pseudomonas could be enriched by application of amino acids, that were present at higher concentrations in the rhizosphere of cabbage plants suffering from insect herbivory. Notably, cabbage plants exhibited the highest resistance to insect herbivory following the application of a synbiotic, a combination of amino acids (prebiotics) and Pseudomonas spp. (probiotics). Moreover, we show that Pseudomonas activates the jasmonate signaling pathway in the plant. This activation occurred in SA-deficient but not in JA-deficient Arabidopsis thaliana mutants, and led to the induction of glucosinolates-based defenses that are well known to act against insect herbivory. Collectively, this work reveals a belowground \"cry for help\" response in plants induced by aboveground herbivory, which guided the development of a novel synbiotic for plant health maintenance.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The RLI1-OsPUB77-OsBZR3 module mediates the crosstalk between phosphate starvation and brassinosteroid signaling pathways to shape rice shoot architecture. RLI1-OsPUB77-OsBZR3模块介导磷酸盐饥饿和油菜素内酯信号通路之间的串音，从而形成水稻茎部结构。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-27 DOI: 10.1016/j.molp.2025.09.019

Kai Wang, Peng Yan, Jiangfan Guo, Wei Li, Shichen Zhou, Yijian Liu, Jiming Xu, Yu Liu, Yunrong Wu, Zhongchang Wu, Peng Wang, Chuanzao Mao, Xiaorong Mo

{"title":"The RLI1-OsPUB77-OsBZR3 module mediates the crosstalk between phosphate starvation and brassinosteroid signaling pathways to shape rice shoot architecture.","authors":"Kai Wang, Peng Yan, Jiangfan Guo, Wei Li, Shichen Zhou, Yijian Liu, Jiming Xu, Yu Liu, Yunrong Wu, Zhongchang Wu, Peng Wang, Chuanzao Mao, Xiaorong Mo","doi":"10.1016/j.molp.2025.09.019","DOIUrl":"https://doi.org/10.1016/j.molp.2025.09.019","url":null,"abstract":"Plant architecture is a critical agronomic trait directly affecting planting density and crop yield. Phosphate starvation in rice (Oryza sativa) leads to a significant reduction in tiller number and a more upright leaf angle. Insensitivity to brassinosteroid (BR) signalling can lead to similar phenotypes. However, the underlying molecular mechanism of phosphate affecting plant architecture through brassinosteroid signalling remains obscure. Here, we demonstrate that the phosphate signalling-related transcription factor RLI1a releases its repression of the novel E3 ligase OsPUB77 gene under phosphate deficiency (Pi deficiency). The accumulated OsPUB77 mediates plant architecture by ubiquitinating OsBZR3 to inhibit BR signaling. Furthermore, natural variation in two single-nucleotide polymorphisms within the OsPUB77 U-box domain coding OsPUB77R530 results in higher ubiquitin transfer activity than OsPUB77I530 due to a stronger interaction with E2. Introducing the OsPUB77pro::OsPUB77R530I transgene into the ospub77-1 background confirmed that OsPUB77R530 results in more upright leaves. Our findings reveal the RLI1a-OsPUB77-OsBZR3 module between Pi and BR that modulates plant architecture in response to Pi starvation in rice.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An incoherent feed-forward loop coordinates nitrate uptake and tillering in wheat. 一个非相干的前馈循环协调了小麦对硝态氮的吸收和分蘖。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-27 DOI: 10.1016/j.molp.2025.09.020

Weiya Xu, Yongming Chen, Yanxiao Niu, Bin Liu, Dejie Du, Xining Ning, Tong Huan, Yilan Zhou, Wensheng Ke, Lingfeng Miao, Weilong Guo, Mingming Xin, Yingyin Yao, Huiru Peng, Mingshan You, Zhongfu Ni, Qixin Sun, Jiewen Xing

{"title":"An incoherent feed-forward loop coordinates nitrate uptake and tillering in wheat.","authors":"Weiya Xu, Yongming Chen, Yanxiao Niu, Bin Liu, Dejie Du, Xining Ning, Tong Huan, Yilan Zhou, Wensheng Ke, Lingfeng Miao, Weilong Guo, Mingming Xin, Yingyin Yao, Huiru Peng, Mingshan You, Zhongfu Ni, Qixin Sun, Jiewen Xing","doi":"10.1016/j.molp.2025.09.020","DOIUrl":"https://doi.org/10.1016/j.molp.2025.09.020","url":null,"abstract":"Since the Green Revolution, nitrogen fertilizer has boosted wheat yields, but gains have plateaued. Excessive nitrogen reduces nitrogen use efficiency (NUE) by promoting nonproductive tillers, and balancing nitrogen uptake with tillering remains a challenge in wheat. Here, we identified TaNLP3 as a master regulator of nitrate signaling that, with the SWI/SNF complex, regulates chromatin accessibility to fine-tune nitrate uptake and tiller formation via a temporal transcriptional cascade. In short-term nitrate signaling, TaNLP3 activates the expression of primary nitrate response (PNR) genes, including TaNRT2.1, to promote nitrate uptake. In long-term nitrate signaling, TaLBD38 induced by TaNLP3 represses TaNRT2.1, limiting nitrate uptake, while promoting tillering by inhibiting TaCKX4/5, negative modulators of tillering. We further identified elite haplotypes of TaNLP3-3B, TaLBD38-4A, and TaNRT2.1-6B4 that enable higher yield under equivalent nitrogen supply, offering valuable resources for breeding wheat varieties with improved NUE. Together, our findings reveal the dynamic coordination between nitrate uptake and tillering under fluctuating nitrogen conditions, offering insights for the sustainable improvement of wheat productivity.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The OsIMP1-OsRLK5 immunopeptide-receptor pair confers bacterial and fungal disease resistance in rice. OsIMP1-OsRLK5免疫肽受体对赋予水稻对细菌和真菌疾病的抗性。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-23 DOI: 10.1016/j.molp.2025.09.015

Liangpeng Sun, Nan Nan, Minxuan Jiang, Jiaqing Xu, Zhicheng Huang, Jiyun Yang, Lina Ji, Jialin Zhang, Jiyang Wang, Dan Zhao, Ling Liu, Guohua Duan, Dayong Li, Wenxian Sun

{"title":"The OsIMP1-OsRLK5 immunopeptide-receptor pair confers bacterial and fungal disease resistance in rice.","authors":"Liangpeng Sun, Nan Nan, Minxuan Jiang, Jiaqing Xu, Zhicheng Huang, Jiyun Yang, Lina Ji, Jialin Zhang, Jiyang Wang, Dan Zhao, Ling Liu, Guohua Duan, Dayong Li, Wenxian Sun","doi":"10.1016/j.molp.2025.09.015","DOIUrl":"https://doi.org/10.1016/j.molp.2025.09.015","url":null,"abstract":"Receptor-like kinases (RLKs) often function as immune receptors of exogenous and endogenous elicitors in plants. However, only a few immune ligand-receptor pairs have been identified in rice. In this study, we report that a rice gene encoding the secretory immunopeptide OsIMP1 is induced by pathogen-associated molecular patterns. The peptide triggers immune responses and positively regulates rice disease resistance. Furthermore, we find that the leucine-rich repeat receptor-like kinases OsRLK5, OsRLK5-L, and somatic embryogenesis receptor-like kinase 2 (OsSERK2) are essential for OsIMP1-triggered immunity. OsRLK5 interacts with and phosphorylates OsSERK2. OsIMP1 directly binds to the extracellular domain of OsRLK5 that positively regulates pattern-triggered immunity. Notably, overexpression of OsRLK5 confers increased resistance to bacterial and fungal diseases without yield penalty in rice. Collectively, this study reveals that OsIMP1 and OsRLK5/ OsRLK5-L form ligand-receptor pairs that modulate multiple disease resistance and uncovers an important strategy how rice plants defend against multiple pathogens without growth penalty.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative gene regulatory networks and machine learning unveil the functions of novel maize regulators. 整合基因调控网络和机器学习揭示了新型玉米调控因子的功能。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-20 DOI: 10.1016/j.molp.2025.09.014

Klaas Vandepoele

引用次数: 0

Scalable AI-Driven Protein Engineering with AiCE. 可扩展的ai驱动蛋白质工程与AiCE。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-18 DOI: 10.1016/j.molp.2025.09.002

Rui Deng, Xiangtan Chen, Alisdair R Fernie, Youjun Zhang

引用次数: 0

The BIK1-mediated phosphorylation and SCF^SKIP31-mediated ubiquitination coordinately control CNGC3 homeostasis and channel activity to fine tune plant immunity. bik1介导的磷酸化和scfskip31介导的泛素化协同控制CNGC3的稳态和通道活性，微调植物免疫。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-15 DOI: 10.1016/j.molp.2025.09.012

Hang Yi, Meng-Jiao Liu, You-Ping Xu, Xin-Zhong Cai

{"title":"The BIK1-mediated phosphorylation and SCFSKIP31-mediated ubiquitination coordinately control CNGC3 homeostasis and channel activity to fine tune plant immunity.","authors":"Hang Yi, Meng-Jiao Liu, You-Ping Xu, Xin-Zhong Cai","doi":"10.1016/j.molp.2025.09.012","DOIUrl":"https://doi.org/10.1016/j.molp.2025.09.012","url":null,"abstract":"Cyclic nucleotide-gated ion channels (CNGCs) are a key component in the pattern-triggered immunity (PTI) signaling. Tight control of CNGC homeostasis is critical for plant growth-immunity balance. Nevertheless, the mechanisms for fine-tuning CNGC homeostasis remain largely unknown. Here, we report that Arabidopsis thaliana CNGC3 is a functional calcium channel to mediate pattern-induced Ca2+ influx, PTI and resistance to Sclerotinia sclerotiorum. We identified a CNGC interactor, Skp1-interacting protein 31 (SKIP31). In the absence of pathogen, SKIP31 ubiquitinates CNGC3 at Lys8 and Lys33 of the K-X-V-R motifs for degradation to repress plant immunity. When pathogen attacks, activated BOTRYTIS-INDUCED KINASE1 (BIK1) phosphorylates SKIP31 to inhibit its ubiquitin ligase activity and interaction with CNGC3 N-terminal region, thereby suppressing CNGC3 protein degradation to promote immunity. Phosphorylation within the F-box of SKIP31 at Ser88 and Ser93 and that at the C-terminal Ser261 prevent its interaction with Skp1 and CNGC3 respectively. These phosphorylation sites are conserved in SKIP31 of different plant species, and SKIP31 interacts with all examined CNGCs, suggesting the pivotal role of the SKIP31 phosphorylation for CNGC stability and plant immunity. Moreover, BIK1 directly phosphorylates CNGC3 cytoplasmic C-terminal region at four Ser residues to enhance its Ca2+ channel activity, demonstrating the dual roles of BIK1 in both promoting CNGC channel activity and stabilizing the channel protein. Our work unveils an SCF ubiquitin ligase-RLCK control system of CNGCs for plant immunity.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persulfidation of host NADPH oxidase RbohB by rhizobial 3-mercaptopyruvate sulfurtransferase maintains redox homeostasis and promotes symbiotic nodulation in soybean. 根瘤菌3-巯基丙酮酸硫转移酶对宿主NADPH氧化酶RbohB的过硫化作用维持氧化还原稳态，促进大豆共生结瘤。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-15 DOI: 10.1016/j.molp.2025.09.013

Weiqin Zhang, Wuyu Liu, Kai Wang, Huaping Cheng, Xiaoli Bai, Jianhua Zhang, Gehong Wei, Juan Chen

{"title":"Persulfidation of host NADPH oxidase RbohB by rhizobial 3-mercaptopyruvate sulfurtransferase maintains redox homeostasis and promotes symbiotic nodulation in soybean.","authors":"Weiqin Zhang, Wuyu Liu, Kai Wang, Huaping Cheng, Xiaoli Bai, Jianhua Zhang, Gehong Wei, Juan Chen","doi":"10.1016/j.molp.2025.09.013","DOIUrl":"https://doi.org/10.1016/j.molp.2025.09.013","url":null,"abstract":"Reactive oxygen species (ROS) play a crucial role in various stages of legume-rhizobium symbiosis, from initial nodulation signaling to nodule senescence. However, how rhizobial redox-related proteins regulate symbiotic nodulation in legumes remains largely unknown. By combining transcriptomics, proteomics, and biochemical and molecular genetics, we investigated the role of the Sinorhizobium fredii Q8 enzyme 3-mercaptopyruvate sulfurtransferase (3MST). Although 3MST was not the primary source of hydrogen sulfide (H2S) under our conditions, its absence significantly disrupted symbiotic nodule development, redox homeostasis, infection capacity, and nitrogen fixation efficiency in soybean. We identified host plasma membrane-localized NADPH oxidase (RbohB) as a pivotal regulator that activates immune responses during nodule development. Notably, 3MST localized to the nucleoid and cytoplasmic membrane and was secreted during nodulation, where it interacted with RbohB and persulfidated Cys791 to suppress NADPH oxidase activity. This 3MST-mediated regulation maintained symbiotic redox balance and promoted nodule development. Genetic analysis of soybean involving RbohB overexpression, RNA interference and site-directed mutagenesis at Cys791 supported the model, linking the 3MST-RbohB interaction to effective rhizobial colonization and improved plant growth. Collectively, our findings shed light on a rhizobium-host redox pathway in which a rhizobial sulfurtransferase modulates RbohB via persulfidation to promote nodulation.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adenylate cyclases tune heat-dependent stomatal responses. 腺苷酸环化酶调节热依赖性气孔反应。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-12 DOI: 10.1016/j.molp.2025.09.011

Aloysius Wong, Chris Gehring

引用次数: 0

Redefining agroecological zones in China to mitigate climate change impacts on maize production. 重新界定中国农业生态区以减轻气候变化对玉米生产的影响。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-09-10 DOI: 10.1016/j.molp.2025.09.005

Chuan Tang, Chunmeng Wang, Zhenwei Zhang, Yilan Cao, Mustafa Bulut, Yingjie Xiao, Xiaoyun Li, Tao Xiong, Jianbing Yan, Tingting Guo

引用次数: 0