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Nanopore ultra-long sequencing and adaptive sampling spur plant complete telomere-to-telomere genome assembly. 纳米孔超长测序和自适应取样技术促进了植物端粒到端粒基因组的完全组装。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-16 DOI: 10.1016/j.molp.2024.10.008
Dongdong Lu, Caijuan Liu, Wenjun Ji, Ruiyan Xia, Shanshan Li, Yanxia Liu, Naixu Liu, Yongqi Liu, Xing Wang Deng, Bosheng Li
{"title":"Nanopore ultra-long sequencing and adaptive sampling spur plant complete telomere-to-telomere genome assembly.","authors":"Dongdong Lu, Caijuan Liu, Wenjun Ji, Ruiyan Xia, Shanshan Li, Yanxia Liu, Naixu Liu, Yongqi Liu, Xing Wang Deng, Bosheng Li","doi":"10.1016/j.molp.2024.10.008","DOIUrl":"10.1016/j.molp.2024.10.008","url":null,"abstract":"<p><p>The pursuit of complete telomere-to-telomere (T2T) genome assembly in plants, challenged by genomic complexity, has been advanced by Oxford Nanopore Technologies (ONT), which offers ultra-long, real-time sequencing. Despite its promise, sequencing length and gap filling remain significant challenges. This study optimized DNA extraction and library preparation, achieving DNA lengths exceeding 485 kb; average N50 read lengths of 80.57 kb, reaching up to 440 kb; and maximum reads of 5.83 Mb. Importantly, we demonstrated that combining ultra-long sequencing and adaptive sampling can effectively fill gaps during assembly, evidenced by successfully filling the remaining gaps of a near-complete Arabidopsis genome assembly and resolving the sequence of an unknown telomeric region in watermelon genome. Collectively, our strategies improve the feasibility of complete T2T genomic assemblies across various plant species, enhancing genome-based research in diverse fields.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1773-1786"},"PeriodicalIF":17.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Next-generation research on transcriptional regulation of plant immunity. 植物免疫转录调控的新一代研究。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-11 DOI: 10.1016/j.molp.2024.10.005
Akira Mine
{"title":"Next-generation research on transcriptional regulation of plant immunity.","authors":"Akira Mine","doi":"10.1016/j.molp.2024.10.005","DOIUrl":"10.1016/j.molp.2024.10.005","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1651-1653"},"PeriodicalIF":17.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems. 合成生物学系统揭示了角草 Rubiscos 独特的生物生成和动力学过程。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-11-02 DOI: 10.1016/j.molp.2024.10.013
Zhen-Guo Oh, Tanner Ashton Robison, Dan-Hong Loh, Warren Shou Leong Ang, Jediael Ng, Fay-Wei Li, Laura Helen Gunn
{"title":"Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems.","authors":"Zhen-Guo Oh, Tanner Ashton Robison, Dan-Hong Loh, Warren Shou Leong Ang, Jediael Ng, Fay-Wei Li, Laura Helen Gunn","doi":"10.1016/j.molp.2024.10.013","DOIUrl":"https://doi.org/10.1016/j.molp.2024.10.013","url":null,"abstract":"<p><p>Hornworts are the only land plants that employ a pyrenoid to optimize Rubisco's CO<sub>2</sub> fixation. Yet, hornwort Rubisco remains poorly characterized. Here we assemble the hornwort Anthoceros agrestis Rubisco (AaRubisco) using the Arabidopsis thaliana SynBio expression system and observed the formation of stalled intermediates, prompting us to develop a new SynBio system with A. agrestis cognate chaperones. We successfully assembled AaRubisco and Rubisco from three other hornwort species. Unlike A. thaliana Rubisco, AaRubisco assembly is not dependent on RbcX or Raf2. Kinetic characterization reveals that hornwort Rubiscos exhibit a range of catalytic rates (3-10 s<sup>-1</sup>), but with similar affinity (∼30 μM) and specificity (∼70) for CO<sub>2</sub>. In other words, hornwort Rubiscos do not comply with the long held canonical catalytic trade-off observed in other land plants, providing experimental support that Rubisco kinetics may be phylogenetically constrained. Unexpectedly, we observed a 50% increase in AaRubisco catalytic rates when RbcX was removed from our SynBio system, without any reduction in specificity. Structural biology, biochemistry and proteomic analysis suggest that subtle differences in Rubisco large subunit interactions, when RbcX is absent during biogenesis, increases the accessibility of active sites and catalytic turnover rate. This study uncovered a previously unknown Rubisco kinetic parameter space and provides a SynBio chassis to expand the survey of other Rubisco kinetics. Our discovery could thus reshape the approaches for engineering Rubisco with superior kinetics.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural variation in maize ZmLecRK1 fine-tunes co-receptor interactions to boost immunity. 玉米 ZmLecRK1 的自然变异可微调共受体的相互作用，从而增强免疫力。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-10-30 DOI: 10.1016/j.molp.2024.10.012
Miguel-Ángel Torres, Lucía Jordá
{"title":"Natural variation in maize ZmLecRK1 fine-tunes co-receptor interactions to boost immunity.","authors":"Miguel-Ángel Torres, Lucía Jordá","doi":"10.1016/j.molp.2024.10.012","DOIUrl":"https://doi.org/10.1016/j.molp.2024.10.012","url":null,"abstract":"<p><p>Pattern recognition receptors (PRRs) are integral to plant immunity, functioning as the first line of defense against pathogens. Among these, receptor kinases (RKs) play a critical role in recognizing external signals and initiating immune responses. Recent studies by Li et al. (2024) have identified the G-type lectin receptor kinase ZmLecRK1 in maize as essential for resistance to Pythium aphanidermatum and other fungal pathogens. A key finding is that a resistant variant of ZmLecRK1, which carries an alanine at position 404, has emerged in maize, while the ancestral form, with a serine at this position, is conserved across most grasses. This amino acid substitution significantly influences the interaction with the co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 (BAK1), enhancing immune complex formation and subsequent defense signaling. This work underscores the importance of genetic variation in enhancing disease resistance, offering potential strategies for crop improvement through targeted genetic modification.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SCOOP10 And SCOOP12 Peptides Act Through MIK2 Receptor-Like Kinase to Antagonistically Regulate Arabidopsis Leaf Senescence. SCOOP10 和 SCOOP12 肽通过 MIK2 受体样激酶拮抗拟南芥叶片衰老。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-10-28 DOI: 10.1016/j.molp.2024.10.010
Zhenbiao Zhang, Nora Gigli-Bisceglia, Wei Li, Saijie Li, Jie Wang, Junfeng Liu, Christa Testerink, Yongfeng Guo
{"title":"SCOOP10 And SCOOP12 Peptides Act Through MIK2 Receptor-Like Kinase to Antagonistically Regulate Arabidopsis Leaf Senescence.","authors":"Zhenbiao Zhang, Nora Gigli-Bisceglia, Wei Li, Saijie Li, Jie Wang, Junfeng Liu, Christa Testerink, Yongfeng Guo","doi":"10.1016/j.molp.2024.10.010","DOIUrl":"https://doi.org/10.1016/j.molp.2024.10.010","url":null,"abstract":"<p><p>Leaf senescence plays a critical role in a plant's overall reproductive success due to its involvement in nutrient remobilization and allocation. However, our current understanding of the molecular mechanisms controlling leaf senescence remains limited. In this study, we demonstrate that the receptor-like kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) functions as a negative regulator of leaf senescence. We report that the SERINE-RICH ENDOGENOUS PEPTIDE 12, previously known to physically interact with MIK2, competes with SCOOP10 to control MIK2-dependent senescence regulatory mechanisms. We observed that increased expression of SCOOP10 or the application of exogenous SCOOP10 peptides accelerated leaf senescence in a MIK2-dependent manner. Conversely, SCOOP12 acted as a suppressor of MIK2-dependent senescence regulation. We also found that SCOOP12 enhanced while SCOOP10 diminished MIK2 phosphorylation. Thus, the SCOOP12-MIK2 module might function antagonistically on SCOOP10-MIK2 signaling at late senescing stages, allowing for fine-tuned modulation of the leaf senescence process. Our research sheds light on the complex mechanisms underlying leaf senescence and provides valuable insights into the interplay between receptors, peptides, and the regulation of plant senescence.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heat stress-induced decapping of WUSCHEL mRNA enhances stem cell thermotolerance in Arabidopsis. 热胁迫诱导的 WUSCHEL mRNA 脱帽可增强拟南芥干细胞的耐热性。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-10-28 DOI: 10.1016/j.molp.2024.10.011
Sumei Liu, Haijun Wu, Zhong Zhao
{"title":"Heat stress-induced decapping of WUSCHEL mRNA enhances stem cell thermotolerance in Arabidopsis.","authors":"Sumei Liu, Haijun Wu, Zhong Zhao","doi":"10.1016/j.molp.2024.10.011","DOIUrl":"https://doi.org/10.1016/j.molp.2024.10.011","url":null,"abstract":"<p><p>The plasticity of stem cells in response to environmental change is critical for multicellular organisms. Here, we show that MYB3R-like directly activates the key plant stem cell regulator WUSCHEL (WUS) by recruiting the methyltransferase ROOT INITIATION DEFECTIVE 2 (RID2), which functions in m7G methylation at the 5' cap of WUS mRNA to protect it from degradation. We demonstrated that protein-folding genes are repressed by WUS to maintain precise protein synthesis in stem cells by preventing the reuse of misfolded proteins. However, upon heat stress, the MYB3R-like/RID2 module is repressed to reduce WUS transcripts via the decapping of nascent WUS mRNA. This releases the inhibition of protein folding capacity in stem cells and protects plant stem cells from heat-shock by eliminating misfolded protein aggregation. Our results reveal a tradeoff strategy in plants by reducing the accuracy of protein synthesis in exchange for the survival of stem cells at high temperatures.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
H2O2 sulfenylates CHE to activate systemic salicylic acid synthesis and ignite systemic acquired resistance. H2O2 亚磺酰化 CHE，激活全身水杨酸合成，点燃全身获得性抵抗。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-10-17 DOI: 10.1016/j.molp.2024.10.007
Kaihuai Li, Cheng Li, Daowen Wang, Fengquan Liu, Zheng Qing Fu
{"title":"H<sub>2</sub>O<sub>2</sub> sulfenylates CHE to activate systemic salicylic acid synthesis and ignite systemic acquired resistance.","authors":"Kaihuai Li, Cheng Li, Daowen Wang, Fengquan Liu, Zheng Qing Fu","doi":"10.1016/j.molp.2024.10.007","DOIUrl":"10.1016/j.molp.2024.10.007","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Upland rice retains genetic elements conferring drought adaptation. 陆地水稻保留了赋予干旱适应性的遗传元素。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-10-11 DOI: 10.1016/j.molp.2024.10.006
Zeqi Li, Yi Zhang, Hao Du
{"title":"Upland rice retains genetic elements conferring drought adaptation.","authors":"Zeqi Li, Yi Zhang, Hao Du","doi":"10.1016/j.molp.2024.10.006","DOIUrl":"10.1016/j.molp.2024.10.006","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increasing seed lint fiber density for promoting cotton yield: Opportunities and challenges. 提高籽棉纤维密度以提高棉花产量：机遇与挑战。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-09-02 DOI: 10.1016/j.molp.2024.08.010
Tianlun Zhao, Jinhong Chen, Shuijin Zhu, Qian-Hao Zhu
{"title":"Increasing seed lint fiber density for promoting cotton yield: Opportunities and challenges.","authors":"Tianlun Zhao, Jinhong Chen, Shuijin Zhu, Qian-Hao Zhu","doi":"10.1016/j.molp.2024.08.010","DOIUrl":"10.1016/j.molp.2024.08.010","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1490-1493"},"PeriodicalIF":17.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Creation of folate-biofortified rice by simultaneously enhancing biosynthetic flux and blocking folate oxidation. 通过同时提高生物合成通量和阻止叶酸氧化，生产叶酸生物强化水稻。
IF 17.1 1区 生物学
Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-09-16 DOI: 10.1016/j.molp.2024.09.005
Qiuju Liang, Wei Zhang, Jianzhou Pang, Shuncong Zhang, Xiaowan Hou, Chunyi Zhang
{"title":"Creation of folate-biofortified rice by simultaneously enhancing biosynthetic flux and blocking folate oxidation.","authors":"Qiuju Liang, Wei Zhang, Jianzhou Pang, Shuncong Zhang, Xiaowan Hou, Chunyi Zhang","doi":"10.1016/j.molp.2024.09.005","DOIUrl":"10.1016/j.molp.2024.09.005","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1487-1489"},"PeriodicalIF":17.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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