Molecular Plant最新文献_第3页

Cytokinin signaling connects the chloroplast function with nuclear genome stability. 细胞分裂素信号将叶绿体功能与核基因组稳定性联系起来。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-28 DOI: 10.1016/j.molp.2025.03.016

Elody Vallet, Cécile Raynaud

引用次数: 0

Low-cadmium rice for food safety: OsCS1 is a genetic breakthrough. 低镉大米促进食品安全：OsCS1是一项基因突破。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-25 DOI: 10.1016/j.molp.2025.03.013

Iqra Noor, Hamza Sohail, Biao Jin, Changquan Zhang

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A highly efficient and environmentally friendly strategy to obtain transgene-free genome-edited progeny in flowering plants. 一种在开花植物中获得无转基因基因组编辑后代的高效环保策略。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-24 DOI: 10.1016/j.molp.2025.03.014

Tianhe Cheng, Meng-Xiang Sun

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SPOTLIGHT: Design of future climate smart crops by engineering heat stress-responsive gene expression. 焦点：通过工程热应激反应基因表达设计未来气候智能型作物。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-21 DOI: 10.1016/j.molp.2025.03.012

Alisdair R Fernie, Mustafa Bulut

引用次数: 0

Dynamic transcriptome and GWAS uncover that a hydroxyproline-rich glycoprotein suppresses Agrobacterium-mediated transformation in maize. 动态转录组和GWAS揭示了富含羟脯氨酸的糖蛋白抑制农杆菌介导的玉米转化。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-19 DOI: 10.1016/j.molp.2025.03.011

Min Liu, Yan Yang, Tianhu Liang, Fengxia Hou, Minyan Zhang, Shijiang He, Peng Liu, Chaoying Zou, Langlang Ma, Guangtang Pan, Yaou Shen

{"title":"Dynamic transcriptome and GWAS uncover that a hydroxyproline-rich glycoprotein suppresses Agrobacterium-mediated transformation in maize.","authors":"Min Liu, Yan Yang, Tianhu Liang, Fengxia Hou, Minyan Zhang, Shijiang He, Peng Liu, Chaoying Zou, Langlang Ma, Guangtang Pan, Yaou Shen","doi":"10.1016/j.molp.2025.03.011","DOIUrl":"https://doi.org/10.1016/j.molp.2025.03.011","url":null,"abstract":"Genetic transformation is a crucial tool for investigating gene function and advancing molecular breeding in crops, with Agrobacterium tumefaciens-mediated transformation being the primary method for plant genetic modification. However, this approach exhibits significant genotypic dependence in maize. Therefore, to overcome these limitations, we herein combined dynamic transcriptome analysis and genome-wide association study (GWAS) to identify the key genes controlling Agrobacterium infection frequency (AIF) in immature maize embryos. Transcriptome analysis of Agrobacterium-infected embryos uncovered 8483 and 1580 genotype-specific response genes in 18-599R (low AIF) and A188 (high AIF), respectively. A weighted gene co-expression network analysis (WGCNA) further revealed five and seven stage-specific co-expression modules in each corresponding line. Basd on a self-developed AIF quantitation method, the GWAS revealed 30 AIF-associated single nucleotide polymorphisms and 315 candidate genes under multiple environments. Integration of GWAS and WGCNA further identified 12 key genes associated with high AIF in A188, among which, ZmHRGP, encoding a hydroxyproline-rich glycoprotein, was functionally validated as a key factor of AIF in immature embryos. Knockout of ZmHRGP further enabled to establish a high-efficiency genetic transformation system for the 18-599R line, with the transformation frequency being approximately 80%. Moreover, transient reduction of ZmHRGP expression significantly enhanced the AIF of maize calluses and leaves. Overall, these findings advance our understanding of plant factors controlling Agrobacterium infection and contribute to the development of more efficient Agrobacterium-mediated transformation systems in crops.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antisense-mediated regulation of nitric oxide biosynthesis. 一氧化氮生物合成的反义调控。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-17 DOI: 10.1016/j.molp.2025.03.010

Wil Prall, Martin Crespi

引用次数: 0

Propagation of plant immunity via interactions between PRIMER and bystander cells. 通过 PRIMER 和旁观者细胞之间的相互作用传播植物免疫力。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-14 DOI: 10.1016/j.molp.2025.03.009

Hongyuan Zheng, Yu Wang, Xuemin Zhou, Daowen Wang, Shi Xiao, Zheng Qing Fu

引用次数: 0

Single-plant NLR is able to recognize effectors from a wide range of adapted and non-adapted pathogens. 单株植物 NLR 能够识别来自各种适应性和非适应性病原体的效应物。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-13 DOI: 10.1016/j.molp.2025.03.008

Soohyun Oh, Doil Choi

引用次数: 0

DNA damage triggers heritable alterations in DNA methylation patterns in Arabidopsis. DNA损伤引发拟南芥DNA甲基化模式的遗传改变

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-03 Epub Date: 2025-01-24 DOI: 10.1016/j.molp.2025.01.019

Jinchao Li, Wenjie Liang, Xin-Qiang He, Weiqiang Qian

{"title":"DNA damage triggers heritable alterations in DNA methylation patterns in Arabidopsis.","authors":"Jinchao Li, Wenjie Liang, Xin-Qiang He, Weiqiang Qian","doi":"10.1016/j.molp.2025.01.019","DOIUrl":"10.1016/j.molp.2025.01.019","url":null,"abstract":"It has been hypothesized that DNA damage has the potential to induce DNA hypermethylation, contributing to carcinogenesis in mammals. However, there is no sufficient evidence to support that DNA damage can cause genome-wide DNA hypermethylation. In this study, we demonstrated that DNA single-strand breaks with 3' blocked ends (DNA 3' blocks) not only can reinforce DNA methylation at normally methylated loci but also can induce DNA methylation at normally nonmethylated loci in plants. The CG and CHG hypermethylation tend to localize within gene bodies, with a significant proportion being de novo generated. In contrast, the CHH hypermethylation is concentrated in centromeric and pericentromeric regions, primarily being reinforced methylation. Mechanistically, DNA 3' blocks regulate the DREAM complex to induce CG and CHG methylation. Moreover, they utilize the RdDM pathway to induce CHH hypermethylation. Intriguingly, repair of DNA damage or blocking the DNA damage response can fully abolish CHH hypermethylation and partially rescue CHG hypermethylation but rarely alter CG hypermethylation, indicating that DNA damage-induced symmetric DNA methylation can serve as a form of genetic imprinting. Collectively, these results suggest that DNA damage is an important force driving the emergence and evolution of genomic DNA methylation levels and patterns in plants.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"501-512"},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143040166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plant retromer components SNXs bind to ATG8 and CLASP to mediate autophagosome movement along microtubules. 植物逆转录成分SNXs与ATG8和CLASP结合介导自噬体沿微管运动。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-03-03 Epub Date: 2024-12-24 DOI: 10.1016/j.molp.2024.12.013

Yanglan Liao, Xibao Li, Wenlong Ma, Xinyi Lin, Jiayi Kuang, Xuanang Zheng, Zien Li, Fanfan Qiao, Chuanliang Liu, Jun Zhou, Faqiang Li, Ruixi Li, Byung-Ho Kang, Hongbo Li, Caiji Gao

{"title":"The plant retromer components SNXs bind to ATG8 and CLASP to mediate autophagosome movement along microtubules.","authors":"Yanglan Liao, Xibao Li, Wenlong Ma, Xinyi Lin, Jiayi Kuang, Xuanang Zheng, Zien Li, Fanfan Qiao, Chuanliang Liu, Jun Zhou, Faqiang Li, Ruixi Li, Byung-Ho Kang, Hongbo Li, Caiji Gao","doi":"10.1016/j.molp.2024.12.013","DOIUrl":"10.1016/j.molp.2024.12.013","url":null,"abstract":"In eukaryotic cells, autophagosomes are double-membrane vesicles that are highly mobile and traffic along cytoskeletal tracks. While core autophagy-related proteins (ATGs) and other regulators involved in autophagosome biogenesis in plants have been extensively studied, the specific components regulating plant autophagosome motility remain elusive. In this study, using TurboID-based proximity labeling, we identify the retromer subcomplex comprising sorting nexin 1 (SNX1), SNX2a, and SNX2b as interacting partners of ATG8. Remarkably, SNX proteins decorate ATG8-labeled autophagosomes and facilitate their coordinated movement along microtubules. Depletion of SNX proteins restricts the motility of autophagosomes in the cytoplasm, resulting in decreased autophagic flux. Furthermore, we show that the microtubule-associated protein CLASP is a bridge, connecting the SNX-ATG8-decorated autophagosomes to the microtubules. Genetically, the clasp-1 mutant phenotype resembles that of plants with disrupted SNXs or microtubule networks, displaying diminished autophagosome motility and reduced autophagic flux. Collectively, our study unveils a hitherto unanticipated role of the SNXs subcomplex in connecting autophagosomes with microtubules to promote autophagosome mobility in Arabidopsis.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"416-436"},"PeriodicalIF":17.1,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0