Molecular Plant最新文献_第3页

The OsSRO1c-OsDREB2B complex undergoes protein phase transition to enhance cold tolerance in rice. OsSRO1c-OsDREB2B 复合物经历蛋白质相变以增强水稻的耐寒性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-21 DOI: 10.1016/j.molp.2024.08.006

Dan Hu, Yilong Yao, Yan Lv, Jun You, Yu Zhang, Qingya Lv, Jiawei Li, Stephanie Hutin, Haiyan Xiong, Chloe Zubieta, Xuelei Lai, Lizhong Xiong

{"title":"The OsSRO1c-OsDREB2B complex undergoes protein phase transition to enhance cold tolerance in rice.","authors":"Dan Hu, Yilong Yao, Yan Lv, Jun You, Yu Zhang, Qingya Lv, Jiawei Li, Stephanie Hutin, Haiyan Xiong, Chloe Zubieta, Xuelei Lai, Lizhong Xiong","doi":"10.1016/j.molp.2024.08.006","DOIUrl":"https://doi.org/10.1016/j.molp.2024.08.006","url":null,"abstract":"Cold stress is one of the major abiotic stress factors affecting rice growth and development, leading to significant yield loss in the context of global climate change. Exploring natural variants that confer cold resistance and the underlying molecular mechanism responsible for this is the major strategy to breed cold tolerant rice varieties. Here, we show that the natural variations of a SIMILAR to RCD ONE (SRO) gene, OsSRO1c, confer cold tolerance in rice at both seedling and booting stages. OsSRO1c possesses intrinsic liquid-liquid phase separation ability in vivo and in vitro and recruits an AP2/ERF transcription factor and positive cold stress regulator, OsDREB2B, into its biomolecular condensates in the nucleus, resulting in elevated transcriptional activity of OsDREB2B. The OsSRO1c-OsDREB2B complex directly responds to low temperature through dynamic phase transitions and regulates key cold response genes, including COLD1. Furthermore, introgression of an elite haplotype of OsSRO1c into a cold susceptible indica rice significantly increases its cold resistance. Collectively, our work reveals a novel cold tolerance regulatory module in rice and provides promising genetic targets for molecular breeding of cold-tolerant rice varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The strigolactone-gibberellin crosstalk mediated by a distant silencer fine-tunes plant height in upland cotton. 由遥远的沉默因子介导的赤霉内酯-赤霉素串扰微调了陆地棉的植株高度。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-20 DOI: 10.1016/j.molp.2024.08.007

Zailong Tian, Baojun Chen, Hongge Li, Xinxin Pei, Yaru Sun, Gaofei Sun, Zhaoe Pan, Panhong Dai, Xu Gao, Xiaoli Geng, Zhen Peng, Yinhua Jia, Daowu Hu, Liru Wang, Baoyin Pang, Ai Zhang, Xiongming Du, Shoupu He

{"title":"The strigolactone-gibberellin crosstalk mediated by a distant silencer fine-tunes plant height in upland cotton.","authors":"Zailong Tian, Baojun Chen, Hongge Li, Xinxin Pei, Yaru Sun, Gaofei Sun, Zhaoe Pan, Panhong Dai, Xu Gao, Xiaoli Geng, Zhen Peng, Yinhua Jia, Daowu Hu, Liru Wang, Baoyin Pang, Ai Zhang, Xiongming Du, Shoupu He","doi":"10.1016/j.molp.2024.08.007","DOIUrl":"https://doi.org/10.1016/j.molp.2024.08.007","url":null,"abstract":"The optimal plant height is crucial in modern agriculture, influencing lodging resistance and facilitating mechanized crop production. Upland cotton (Gossypium hirsutum) is the most crucial fiber crop globally, but the knowledge of the genetic basis underlying plant height still needs to be discovered. Here we conducted a genome-wide association study (GWAS) to identify the major locus controlling plant height (PH1) in upland cotton. The locus encodes gibberellin 2-oxidase 1A (GhPH1), with a 1,133 bp-length structural variation (PAVPH1) located approximately 16 kb upstream of it. The presence or absence of PAVPH1 confers a differential expression of GhPH1, consequently leading to changes in plant height. Further analysis revealed that a gibberellin-regulating transcription factor (GhGARF) recognizes a specific 'CATTTG' motif on the GhPH1 promoter and PAVPH1. This binding event down-regulates GhPH1, indicating that PAVPH1 functions as a distant upstream silencer. Intriguingly, we found that the critical repressor of the strigolactone (SL) signaling pathway, DWARF53 (D53), directly interacts with GhGARF and inhibits its binding to targets. Moreover, our study uncovers a previously unrecognized GA-SL crosstalk mechanism mediated by the GhD53-GhGARF-GhPH1/PAVPH1 module, crucial in regulating the plant height of upland cotton. These findings shed light on the genetic basis and gene interaction network underlying plant height and provide valuable insights for developing semi-dwarf cotton varieties through precise modulation of GhPH1 expression.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient control of root-knot nematodes by expressing Bt nematicidal proteins in root leucoplasts. 通过在根白质中表达 Bt 杀线虫蛋白有效控制根结线虫。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-14 DOI: 10.1016/j.molp.2024.08.004

Yong Wang, Mengnan Wang, Yali Zhang, Longwei Peng, Dadong Dai, Fengjuan Zhang, Jiang Zhang

{"title":"Efficient control of root-knot nematodes by expressing Bt nematicidal proteins in root leucoplasts.","authors":"Yong Wang, Mengnan Wang, Yali Zhang, Longwei Peng, Dadong Dai, Fengjuan Zhang, Jiang Zhang","doi":"10.1016/j.molp.2024.08.004","DOIUrl":"10.1016/j.molp.2024.08.004","url":null,"abstract":"Root-knot nematodes (RKNs) are plant pests that infect the roots of host plants. Bacillus thuringiensis (Bt) nematicidal proteins exhibited toxicity to nematodes. However, the application of nematicidal proteins for plant protection is hampered by the lack of effective delivery systems in transgenic plants. In this study, we discovered the accumulation of leucoplasts (root plastids) in galls and RKN-induced giant cells. RKN infection causes the degradation of leucoplasts into small vesicle-like structures, which are responsible for delivering proteins to RKNs, as observed through confocal microscopy and immunoelectron microscopy. We showed that different-sized proteins from leucoplasts could be taken up by Meloidogyne incognita female. To further explore the potential applications of leucoplasts, we introduced the Bt crystal protein Cry5Ba2 into tobacco and tomato leucoplasts by fusing it with a transit peptide. The transgenic plants showed significant resistance to RKNs. Intriguingly, RKN females preferentially took up Cry5Ba2 protein when delivered through plastids rather than the cytosol. The decrease in progeny was positively correlated with the delivery efficiency of the nematicidal protein. In conclusion, this study offers new insights into the feeding behavior of RKNs and their ability to ingest leucoplast proteins, and demonstrates that root leucoplasts can be used for delivering nematicidal proteins, thereby offering a promising approach for nematode control.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and maintenance of the ligular region of maize leaves. 玉米叶片韧带区的发育和维持。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-11 DOI: 10.1016/j.molp.2024.07.004

Josh Strable, Alejandro Aragón-Raygoza

引用次数: 0

Leveraging plant biomechanics in multiscale plant systems for sustainable innovations. 在多尺度植物系统中利用植物生物力学进行可持续创新。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-05 DOI: 10.1016/j.molp.2024.07.002

Jinbo Shen, Yansong Miao

引用次数: 0

Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple. ACC 氧化酶 3 mRNA 从种子到果肉的移动促进了苹果果实的成熟。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-19 DOI: 10.1016/j.molp.2024.06.008

Ting Wang, Yi Zheng, Chen Xu, Yulin Deng, Xinyi Hao, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu

{"title":"Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple.","authors":"Ting Wang, Yi Zheng, Chen Xu, Yulin Deng, Xinyi Hao, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu","doi":"10.1016/j.molp.2024.06.008","DOIUrl":"10.1016/j.molp.2024.06.008","url":null,"abstract":"Xenia, the phenomenon in which the pollen genotype directly affects the phenotypic characteristics of maternal tissues (i.e., fruit ripening), has applications in crop production and breeding. However, the underlying molecular mechanism has yet to be elucidated. Here, we investigated whether mobile mRNAs from the pollen affect the ripening and quality-related characteristics of the fruit using cross-pollination between distinct Malus domestica (apple) cultivars. We demonstrated that hundreds of mobile mRNAs originating from the seeds are delivered to the fruit. We found that the movement of one of these mRNAs, ACC oxidase 3 (MdACO3), is coordinated with fruit ripening. Salicylic acid treatment, which can cause plasmodesmal closure, blocks MdACO3 movement, indicating that MdACO3 transcripts may move through the plasmodesmata. To assess the role of mobile MdACO3 transcripts in apple fruit, we created MdACO3-GFP-expressing apple seeds using MdACO3-GFP-overexpressing pollen for pollination and showed that MdACO3 transcripts in the transgenic seeds move to the flesh, where they promote fruit ripening. Furthermore, we demonstrated that MdACO3 can be transported from the seeds to fruit in the fleshy-fruited species tomato and strawberry. These results underscore the potential of mobile mRNAs from seeds to influence fruit characteristics, providing an explanation for the xenia phenomenon. Notably, our findings highlight the feasibility of leveraging diverse pollen genomic resources, without resorting to genome editing, to improve fruit quality.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141432352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A near-complete cucumber reference genome assembly and Cucumber-DB, a multi-omics database. 近乎完整的黄瓜参考基因组组装和多组学数据库 Cucumber-DB。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-20 DOI: 10.1016/j.molp.2024.06.012

Jiantao Guan, Han Miao, Zhonghua Zhang, Shaoyun Dong, Qi Zhou, Xiaoping Liu, Diane M Beckles, Xingfang Gu, Sanwen Huang, Shengping Zhang

引用次数: 0

Phenotyping floral attractiveness to pollinators using volatilomics, 3D imaging, and insect monitoring. 利用挥发物组学、三维成像和昆虫监测对花朵对传粉昆虫的吸引力进行表型分析。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-20 DOI: 10.1016/j.molp.2024.06.011

Filip Slavković, Adnane Boualem, Catherine Dogimont, Abdelhafid Bendahmane

引用次数: 0

Steroidal scaffold decorations in Solanum alkaloid biosynthesis. 茄科生物碱生物合成中的类固醇支架装饰。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-26 DOI: 10.1016/j.molp.2024.06.013

Rosalind Lucier, Mohamed O Kamileen, Yoko Nakamura, Sofiia Serediuk, Ranjit Barbole, Jens Wurlitzer, Maritta Kunert, Sarah Heinicke, Sarah E O'Connor, Prashant D Sonawane

{"title":"Steroidal scaffold decorations in Solanum alkaloid biosynthesis.","authors":"Rosalind Lucier, Mohamed O Kamileen, Yoko Nakamura, Sofiia Serediuk, Ranjit Barbole, Jens Wurlitzer, Maritta Kunert, Sarah Heinicke, Sarah E O'Connor, Prashant D Sonawane","doi":"10.1016/j.molp.2024.06.013","DOIUrl":"10.1016/j.molp.2024.06.013","url":null,"abstract":"Steroidal glycoalkaloids (SGAs) are specialized metabolites produced by hundreds of Solanum species, including important vegetable crops such as tomato, potato, and eggplant. Although it has been known that SGAs play important roles in defense in plants and \"anti-nutritional\" effects (e.g., toxicity and bitterness) to humans, many of these molecules have documented anti-cancer, anti-microbial, anti-inflammatory, anti-viral, and anti-pyretic activities. Among these, α-solasonine and α-solamargine isolated from black nightshade (Solanum nigrum) are reported to have potent anti-tumor, anti-proliferative, and anti-inflammatory activities. Notably, α-solasonine and α-solamargine, along with the core steroidal aglycone solasodine, are the most widespread SGAs produced among the Solanum plants. However, it is still unknown how plants synthesize these bioactive steroidal molecules. Through comparative metabolomic-transcriptome-guided approach, biosynthetic logic, combinatorial expression in Nicotiana benthamiana, and functional recombinant enzyme assays, here we report the discovery of 12 enzymes from S. nigrum that converts the starting cholesterol precursor to solasodine aglycone, and the downstream α-solasonine, α-solamargine, and malonyl-solamargine SGA products. We further identified six enzymes from cultivated eggplant that catalyze the production of α-solasonine, α-solamargine, and malonyl-solamargine SGAs from solasodine aglycone via glycosylation and atypical malonylation decorations. Our work provides the gene tool box and platform for engineering the production of high-value, steroidal bioactive molecules in heterologous hosts using synthetic biology.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixing and matching SMXL proteins to fine-tune strigolactone responses. 混合和匹配 SMXL 蛋白以微调绞股蓝内酯反应。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-18 DOI: 10.1016/j.molp.2024.06.009

Jenna E Hountalas, Shelley Lumba

引用次数: 0