Molecular Plant最新文献_第4页

Barley2035: A decade vision on barley research and breeding. 大麦 2035：大麦研究和育种十年展望。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-16 DOI: 10.1016/j.molp.2024.12.009

Congcong Jiang, Jinhong Kan, Guangqi Gao, Christoph Dockter, Chengdao Li, Wenxue Wu, Ping Yang, Nils Stein

{"title":"Barley2035: A decade vision on barley research and breeding.","authors":"Congcong Jiang, Jinhong Kan, Guangqi Gao, Christoph Dockter, Chengdao Li, Wenxue Wu, Ping Yang, Nils Stein","doi":"10.1016/j.molp.2024.12.009","DOIUrl":"https://doi.org/10.1016/j.molp.2024.12.009","url":null,"abstract":"Barley (Hordeum vulgare ssp. vulgare) is one of the oldest founder crops in early human civilization, and has been widely dispersed around the globe to supply human life through livestock feeding and brewing industries. It has been used in innovative research of cytogenetics, biochemistry, and genetics since the early half of the 20th century, facilitated by its mode of reproduction through self-pollination, its true diploid status which has contributed to the accumulation of a plethora of germplasm and mutant resources. Coming to the era of molecular genomics and biology, a multitude of barley genes and their involved regulatory mechanisms have been uncovered and functionally validated, providing the paradigm for equivalent studies in other Triticeae crops. This review features the advancements over the past decade in barley research, mainly regarding genomics and genomics-assisted germplasm exploration, genetic dissection of developmental and adaptation associated traits, as well as the complex dynamics of yield and quality formation. For the coming decade, the perspective of integration of these innovations in barley research and breeding is promising. Barley is proposed as a reference in Triticeae crops for new gene discovery, functional validation and molecular mechanism dissection. The application of precise genome editing as well as genomic prediction and selection, further enhanced by artificial intelligence-enforced tools and applications, is expected to boost barley improvement, in order to efficiently meet the evolving global demands for this important crop.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PDLLMs: A group of tailored DNA large language models for analyzing plant genomes. PDLLMs：一组用于分析植物基因组的定制DNA大语言模型。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-09 DOI: 10.1016/j.molp.2024.12.006

Guanqing Liu, Long Chen, Yuechao Wu, Yangshuo Han, Yu Bao, Tao Zhang

引用次数: 0

Thermotolerance Through Trade-Off: Decapping WUSCHEL mRNA in Plant Stem Cells. 通过权衡获得的耐热性：在植物干细胞中脱帽WUSCHEL mRNA。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-05 DOI: 10.1016/j.molp.2024.12.004

P Debnath, J J Olas

引用次数: 0

An enhancer-promoter-transcription factor module orchestrates plant immune homeostasis by constraining camalexin biosynthesis. 增强-启动-转录因子模块通过限制camalexin生物合成来协调植物免疫稳态。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-03 DOI: 10.1016/j.molp.2024.12.002

Ying Zhang, Meng Tang, Yi Zhang, Qinglin Cheng, Lijiang Liu, Wei Chen, Jiatao Xie, Jiasen Cheng, Yanping Fu, Bo Li, Daohong Jiang, Xiao Yu

{"title":"An enhancer-promoter-transcription factor module orchestrates plant immune homeostasis by constraining camalexin biosynthesis.","authors":"Ying Zhang, Meng Tang, Yi Zhang, Qinglin Cheng, Lijiang Liu, Wei Chen, Jiatao Xie, Jiasen Cheng, Yanping Fu, Bo Li, Daohong Jiang, Xiao Yu","doi":"10.1016/j.molp.2024.12.002","DOIUrl":"10.1016/j.molp.2024.12.002","url":null,"abstract":"Effective plant defense against pathogens relies on highly coordinated regulation of immune gene expression. Enhancers, as cis-regulatory elements, are indispensable determinants of dynamic gene regulation, but the molecular functions in plant immunity are not well understood. In this study, we identified a novel enhancer, CORE PATTERN-INDUCED ENHANCER 35 (CPIE35), which is rapidly activated upon pathogenic elicitation and negatively regulates antifungal resistance through modulating WRKY15 expression. During immune activation, CPIE35 activates the transcription of WRKY15 by forming chromatin loops with the promoter of WRKY15 in a WRKY18/40/60-, WRKY33-, and MYC2-dependent manner. WRKY15 directly binds to the promoters of PAD3 and GSTU4, suppressing their expression and leading to reduced camalexin synthesis and resistance. Interestingly, CPIE35 region is evolutionarily conserved among Brassicaceae plants, and the CPIE35-WRKY15 module exerts similar functions in Brassica napus to negatively regulate antifungal resistance. Our work reveals the \"enhancer-promoter-transcription factor\" regulatory mechanism in maintenance of immune homeostasis, highlighting the importance and conserved role of enhancers in fine-tuning immune gene expression in plants.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. 拟南芥中由替代剪接产生的诱饵受体可微调细胞分裂素信号。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-05 DOI: 10.1016/j.molp.2024.11.001

Michaela Králová, Ivona Kubalová, Jakub Hajný, Karolina Kubiasová, Karolína Vagaská, Zengxiang Ge, Michelle Gallei, Hana Semerádová, Anna Kuchařová, Martin Hönig, Aline Monzer, Martin Kovačik, Jiří Friml, Ondřej Novák, Eva Benková, Yoshihisa Ikeda, David Zalabák

{"title":"A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis.","authors":"Michaela Králová, Ivona Kubalová, Jakub Hajný, Karolina Kubiasová, Karolína Vagaská, Zengxiang Ge, Michelle Gallei, Hana Semerádová, Anna Kuchařová, Martin Hönig, Aline Monzer, Martin Kovačik, Jiří Friml, Ondřej Novák, Eva Benková, Yoshihisa Ikeda, David Zalabák","doi":"10.1016/j.molp.2024.11.001","DOIUrl":"10.1016/j.molp.2024.11.001","url":null,"abstract":"Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsisthaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int7 results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1850-1865"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new gene for restoring wild abortive-type cytoplasmic male sterility in rice. 恢复水稻野生败育型细胞质雄性不育的新基因

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-09 DOI: 10.1016/j.molp.2024.11.004

Xiaoming Zheng, Jian Sun, Cheng Cheng, Qian Qian

引用次数: 0

Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems. 合成生物学系统揭示了角草 Rubiscos 独特的生物生成和动力学过程。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-02 DOI: 10.1016/j.molp.2024.10.013

Zhen Guo Oh, Tanner Ashton Robison, Dan Hong Loh, Warren Shou Leong Ang, Jediael Zheng Ying Ng, Fay-Wei Li, Laura Helen Gunn

{"title":"Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems.","authors":"Zhen Guo Oh, Tanner Ashton Robison, Dan Hong Loh, Warren Shou Leong Ang, Jediael Zheng Ying Ng, Fay-Wei Li, Laura Helen Gunn","doi":"10.1016/j.molp.2024.10.013","DOIUrl":"10.1016/j.molp.2024.10.013","url":null,"abstract":"Hornworts are the only land plants that employ a pyrenoid to optimize Rubisco's CO2 fixation, yet hornwort Rubisco remains poorly characterized. Here we assembled the hornwort Anthoceros agrestis Rubisco (AaRubisco) using the Arabidopsis thaliana SynBio expression system and observed the formation of stalled intermediates, prompting us to develop a new SynBio system with A. agrestis cognate chaperones. We successfully assembled AaRubisco and Rubisco from three other hornwort species. Unlike A. thaliana Rubisco, AaRubisco assembly is not dependent on RbcX or Raf2. Kinetic characterization reveals that hornwort Rubiscos exhibit a range of catalytic rates (3-10 s-1), but with similar affinity (∼30 μM) and specificity (∼70) for CO2. These results suggest that hornwort Rubiscos do not comply with the long-held canonical catalytic trade-off observed in other land plants, providing experimental support that Rubisco kinetics may be phylogenetically constrained. Unexpectedly, we observed a 50% increase in AaRubisco catalytic rates when RbcX was removed from our SynBio system, without any reduction in specificity. Structural biology, biochemistry, and proteomic analysis suggest that subtle differences in Rubisco large-subunit interactions, when RbcX is absent during biogenesis, increases the accessibility of active sites and catalytic turnover rate. Collectively, this study uncovered a previously unknown Rubisco kinetic parameter space and provides a SynBio chassis to expand the survey of other Rubisco kinetics. Our discoveries will contribute to developing new approaches for engineering Rubisco with superior kinetics.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1833-1849"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H₂O₂ sulfenylates CHE to activate systemic salicylic acid synthesis and ignite systemic acquired resistance. H2O2 亚磺酰化 CHE，激活全身水杨酸合成，点燃全身获得性抵抗。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-17 DOI: 10.1016/j.molp.2024.10.007

Kaihuai Li, Cheng Li, Daowen Wang, Fengquan Liu, Zheng Qing Fu

引用次数: 0

Upland rice retains genetic elements conferring drought adaptation. 陆地水稻保留了赋予干旱适应性的遗传元素。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-11 DOI: 10.1016/j.molp.2024.10.006

Zeqi Li, Yi Zhang, Hao Du

引用次数: 0

Natural variation in maize ZmLecRK1 fine-tunes co-receptor interactions to boost immunity. 玉米 ZmLecRK1 的自然变异可微调共受体的相互作用，从而增强免疫力。