Molecular Plant最新文献_第4页

CONSTANS alters the circadian clock in Arabidopsis thaliana. CONSTANS 改变了拟南芥的昼夜节律。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-17 DOI: 10.1016/j.molp.2024.06.006

Pedro de Los Reyes, Gloria Serrano-Bueno, Francisco J Romero-Campero, He Gao, Jose M Romero, Federico Valverde

{"title":"CONSTANS alters the circadian clock in Arabidopsis thaliana.","authors":"Pedro de Los Reyes, Gloria Serrano-Bueno, Francisco J Romero-Campero, He Gao, Jose M Romero, Federico Valverde","doi":"10.1016/j.molp.2024.06.006","DOIUrl":"10.1016/j.molp.2024.06.006","url":null,"abstract":"Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment. Among these processes, the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks. The photoperiodic pathway, which controls plant response to day length, is one of the most important pathways controlling flowering. In Arabidopsis photoperiodic flowering, CONSTANS (CO) is the central gene activating the expression of the florigen FLOWERING LOCUS T (FT) in the leaves at the end of a long day. The circadian clock strongly regulates CO expression. However, to date, no evidence has been reported regarding a feedback loop from the photoperiod pathway back to the circadian clock. Using transcriptional networks, we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod pathway. Gene expression, chromatin immunoprecipitation experiments, and phenotypic analysis allowed us to elucidate the role of CO over the circadian clock. Plants with altered CO expression showed a different internal clock period, measured by daily leaf rhythmic movements. We showed that CO upregulates the expression of key genes related to the circadian clock, such as CCA1, LHY, PRR5, and GI, at the end of a long day by binding to specific sites on their promoters. Moreover, a high number of PRR5-repressed target genes are upregulated by CO, and this could explain the phase transition promoted by CO. The CO-PRR5 complex interacts with the bZIP transcription factor HY5 and helps to localize the complex in the promoters of clock genes. Taken together, our results indicate that there may be a feedback loop in which CO communicates back to the circadian clock, providing seasonal information to the circadian system.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic insights into adaptation of alfalfa. 对紫花苜蓿适应性的遗传学见解。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-28 DOI: 10.1016/j.molp.2024.06.015

Yafei Guo, Lipeng Kang, Fei Lu

引用次数: 0

Phytochrome B interacts with LIGULELESS1 to control plant architecture and density tolerance in maize. 植物色素 B 与 LIGULELESS1 相互作用，控制玉米的植株结构和耐密度。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-29 DOI: 10.1016/j.molp.2024.06.014

Qingbiao Shi, Ying Xia, Qibin Wang, Kaiwen Lv, Hengjia Yang, Lianzhe Cui, Yue Sun, Xiaofei Wang, Qing Tao, Xiehai Song, Di Xu, Wenchang Xu, Xingyun Wang, Xianglan Wang, Fanying Kong, Haisen Zhang, Bosheng Li, Pinghua Li, Haiyang Wang, Gang Li

{"title":"Phytochrome B interacts with LIGULELESS1 to control plant architecture and density tolerance in maize.","authors":"Qingbiao Shi, Ying Xia, Qibin Wang, Kaiwen Lv, Hengjia Yang, Lianzhe Cui, Yue Sun, Xiaofei Wang, Qing Tao, Xiehai Song, Di Xu, Wenchang Xu, Xingyun Wang, Xianglan Wang, Fanying Kong, Haisen Zhang, Bosheng Li, Pinghua Li, Haiyang Wang, Gang Li","doi":"10.1016/j.molp.2024.06.014","DOIUrl":"10.1016/j.molp.2024.06.014","url":null,"abstract":"Over the past few decades, significant improvements in maize yield have been largely attributed to increased plant density of upright hybrid varieties rather than increased yield per plant. However, dense planting triggers shade avoidance responses (SARs) that optimize light absorption but impair plant vigor and performance, limiting yield improvement through increasing plant density. In this study, we demonstrated that high-density-induced leaf angle narrowing and stem/stalk elongation are largely dependent on phytochrome B (phyB1/B2), the primary photoreceptor responsible for perceiving red (R) and far-red (FR) light in maize. We found that maize phyB physically interacts with the LIGULELESS1 (LG1), a classical key regulator of leaf angle, to coordinately regulate plant architecture and density tolerance. The abundance of LG1 is significantly increased by phyB under high R:FR light (low density) but rapidly decreases under low R:FR light (high density), correlating with variations in leaf angle and plant height under various densities. In addition, we identified the homeobox transcription factor HB53 as a target co-repressed by both phyB and LG1 but rapidly induced by canopy shade. Genetic and cellular analyses showed that HB53 regulates plant architecture by controlling the elongation and division of ligular adaxial and abaxial cells. Taken together, these findings uncover the phyB-LG1-HB53 regulatory module as a key molecular mechanism governing plant architecture and density tolerance, providing potential genetic targets for breeding maize hybrid varieties suitable for high-density planting.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SnRK1a1, a new player in the sucrose-Opaque2 network during endosperm filling. SnRK1a1是胚乳填充过程中蔗糖-Opaque2网络中的一个新角色。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-06 DOI: 10.1016/j.molp.2024.07.003

Kelly Mayrink Balmant, Marcio F R Resende

引用次数: 0

Structural variations of a new fertility restorer gene, Rf20, underlie the restoration of wild abortive-type cytoplasmic male sterility in rice. 一种新的生育力恢复基因 Rf20 的结构变异是恢复水稻野生败育型细胞质雄性不育的基础。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-02 DOI: 10.1016/j.molp.2024.07.001

Shufeng Song, Yixing Li, Mudan Qiu, Na Xu, Bin Li, Longhui Zhang, Lei Li, Weijun Chen, Jinglei Li, Tiankang Wang, Yingxin Qiu, Mengmeng Gong, Dong Yu, Hao Dong, Siqi Xia, Yi Pan, Dingyang Yuan, Li Li

{"title":"Structural variations of a new fertility restorer gene, Rf20, underlie the restoration of wild abortive-type cytoplasmic male sterility in rice.","authors":"Shufeng Song, Yixing Li, Mudan Qiu, Na Xu, Bin Li, Longhui Zhang, Lei Li, Weijun Chen, Jinglei Li, Tiankang Wang, Yingxin Qiu, Mengmeng Gong, Dong Yu, Hao Dong, Siqi Xia, Yi Pan, Dingyang Yuan, Li Li","doi":"10.1016/j.molp.2024.07.001","DOIUrl":"10.1016/j.molp.2024.07.001","url":null,"abstract":"The discovery of a wild abortive-type (WA) cytoplasmic male sterile (CMS) line and breeding its restorer line have led to the commercialization of three-line hybrid rice, contributing considerably to global food security. However, the molecular mechanisms underlying fertility abortion and the restoration of CMS-WA lines remain largely elusive. In this study, we cloned a restorer gene, Rf20, following a genome-wide association study analysis of the core parent lines of three-line hybrid rice. We found that Rf20 was present in all core parental lines, but different haplotypes and structural variants of its gene resulted in differences in Rf20 expression levels between sterile and restored lines. Rf20 could restore pollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMS lines under high temperatures. In addition, we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11. This interaction enhances COX11's function as a scavenger of reactive oxygen species, which in turn restores pollen fertility. Collectively, our study suggests a new action mode for pentatricopeptide repeat proteins in the fertility restoration of CMS lines, providing an essential theoretical basis for breeding robust restorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The jasmonate pathway promotes nodule symbiosis and suppresses host plant defense in Medicago truncatula. 茉莉酸盐通路促进麦地那龙葵（Medicago truncatula）的结核共生并抑制宿主植物的防御。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-09 DOI: 10.1016/j.molp.2024.06.004

Da Guo, Jingrui Li, Peng Liu, Yuzhan Wang, Na Cao, Xiangling Fang, Tao Wang, Jiangli Dong

{"title":"The jasmonate pathway promotes nodule symbiosis and suppresses host plant defense in Medicago truncatula.","authors":"Da Guo, Jingrui Li, Peng Liu, Yuzhan Wang, Na Cao, Xiangling Fang, Tao Wang, Jiangli Dong","doi":"10.1016/j.molp.2024.06.004","DOIUrl":"10.1016/j.molp.2024.06.004","url":null,"abstract":"Root nodule symbiosis (RNS) between legumes and rhizobia is a major source of nitrogen in agricultural systems. Effective symbiosis requires precise regulation of plant defense responses. The role of the defense hormone jasmonic acid (JA) in the immune response has been extensively studied. Current research shows that JA can play either a positive or negative regulatory role in RNS depending on its concentration, but the molecular mechanisms remain to be elucidated. In this study, we found that inoculation with the rhizobia Sm1021 induces the JA pathway in Medicago truncatula, and blocking the JA pathway significantly reduces the number of infection threads. Mutations in the MtMYC2 gene, which encodes a JA signaling master transcription factor, significantly inhibited rhizobia infection, terminal differentiation, and symbiotic cell formation. Combining RNA sequencing and chromatin immunoprecipitation sequencing, we discovered that MtMYC2 regulates the expression of nodule-specific MtDNF2, MtNAD1, and MtSymCRK to suppress host defense, while it activates MtDNF1 expression to regulate the maturation of MtNCRs, which in turn promotes bacteroid formation. More importantly, MtMYC2 participates in symbiotic signal transduction by promoting the expression of MtIPD3. Notably, the MtMYC2-MtIPD3 transcriptional regulatory module is specifically present in legumes, and the Mtmyc2 mutants are susceptible to the infection by the pathogen Rhizoctonia solani. Collectively, these findings reveal the molecular mechanisms of how the JA pathway regulates RNS, broadening our understanding of the roles of JA in plant-microbe interactions.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hordedane diterpenoid phytoalexins restrict Fusarium graminearum infection but enhance Bipolaris sorokiniana colonization of barley roots. Hordedane 二萜类植物毒素限制了禾谷镰刀菌的感染，但增强了大麦根部 Bipolaris sorkiniana 的定殖。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-30 DOI: 10.1016/j.molp.2024.07.006

Yaming Liu, Dario Esposto, Lisa K Mahdi, Andrea Porzel, Pauline Stark, Hidayat Hussain, Anja Scherr-Henning, Simon Isfort, Ulschan Bathe, Iván F Acosta, Alga Zuccaro, Gerd U Balcke, Alain Tissier

{"title":"Hordedane diterpenoid phytoalexins restrict Fusarium graminearum infection but enhance Bipolaris sorokiniana colonization of barley roots.","authors":"Yaming Liu, Dario Esposto, Lisa K Mahdi, Andrea Porzel, Pauline Stark, Hidayat Hussain, Anja Scherr-Henning, Simon Isfort, Ulschan Bathe, Iván F Acosta, Alga Zuccaro, Gerd U Balcke, Alain Tissier","doi":"10.1016/j.molp.2024.07.006","DOIUrl":"10.1016/j.molp.2024.07.006","url":null,"abstract":"Plant immunity is a multilayered process that includes recognition of patterns or effectors from pathogens to elicit defense responses. These include the induction of a cocktail of defense metabolites that typically restrict pathogen virulence. Here, we investigate the interaction between barley roots and the fungal pathogens Bipolaris sorokiniana (Bs) and Fusarium graminearum (Fg) at the metabolite level. We identify hordedanes, a previously undescribed set of labdane-related diterpenoids with antimicrobial properties, as critical players in these interactions. Infection of barley roots by Bs and Fg elicits hordedane synthesis from a 600-kb gene cluster. Heterologous reconstruction of the biosynthesis pathway in yeast and Nicotiana benthamiana produced several hordedanes, including one of the most functionally decorated products 19-β-hydroxy-hordetrienoic acid (19-OH-HTA). Barley mutants in the diterpene synthase genes of this cluster are unable to produce hordedanes but, unexpectedly, show reduced Bs colonization. By contrast, colonization by Fusarium graminearum, another fungal pathogen of barley and wheat, is 4-fold higher in the mutants completely lacking hordedanes. Accordingly, 19-OH-HTA enhances both germination and growth of Bs, whereas it inhibits other pathogenic fungi, including Fg. Analysis of microscopy and transcriptomics data suggest that hordedanes delay the necrotrophic phase of Bs. Taken together, these results show that adapted pathogens such as Bs can subvert plant metabolic defenses to facilitate root colonization.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141600789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis WRKY1 promotes monocarpic senescence by integrative regulation of flowering, leaf senescence, and nitrogen remobilization. 拟南芥 WRKY1 通过综合调控开花、叶片衰老和氮素再动员促进单果衰老。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-14 DOI: 10.1016/j.molp.2024.07.005

Wei Zhang, Shufei Tang, Xuying Li, Yuanyuan Chen, Jiajia Li, Yuyang Wang, Ruichao Bian, Ying Jin, Xiaoxian Zhu, Kewei Zhang

{"title":"Arabidopsis WRKY1 promotes monocarpic senescence by integrative regulation of flowering, leaf senescence, and nitrogen remobilization.","authors":"Wei Zhang, Shufei Tang, Xuying Li, Yuanyuan Chen, Jiajia Li, Yuyang Wang, Ruichao Bian, Ying Jin, Xiaoxian Zhu, Kewei Zhang","doi":"10.1016/j.molp.2024.07.005","DOIUrl":"10.1016/j.molp.2024.07.005","url":null,"abstract":"Monocarpic senescence, characterized by whole-plant senescence following a single flowering phase, is widespread in seed plants, particularly in crops, determining seed harvest time and quality. However, how external and internal signals are systemically integrated into monocarpic senescence remains largely unknown. Here, we report that the Arabidopsis thaliana transcription factor WRKY1 plays essential roles in multiple key steps of monocarpic senescence. WRKY1 expression is induced by age, salicylic acid (SA), and nitrogen (N) deficiency. Flowering and leaf senescence are accelerated in the WRKY1 overexpression lines but are delayed in the wrky1 mutants. The combined DNA affinity purification sequencing and RNA sequencing analyses uncover the direct target genes of WRKY1. Further studies show that WRKY1 coordinately regulates three processes in monocarpic senescence: (1) suppressing FLOWERING LOCUS C gene expression to initiate flowering, (2) inducing SA biosynthesis genes to promote leaf senescence, and (3) activating the N assimilation and transport genes to trigger N remobilization. In summary, our study reveals how one stress-responsive transcription factor, WRKY1, integrates flowering, leaf senescence, and N remobilization processes into monocarpic senescence, providing important insights into plant lifetime regulation.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recurrent evolution of seaweed body plan complexity among photosynthetic eukaryotes. 光合真核生物中海藻体表复杂性的反复进化。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-04 DOI: 10.1016/j.molp.2024.06.001

Elisa S Goldbecker, Iker Irisarri, Jan de Vries

引用次数: 0

Plastid engineering with an efficient RNAi delivery system based on bacteriophage MS2 virus-like particles enhances plant resistance to cotton bollworm. 基于噬菌体 MS2 病毒样颗粒的高效 RNAi 传递系统的质体工程可增强植物对棉铃虫的抗性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-04 DOI: 10.1016/j.molp.2024.05.013

Chunmei Jiang, Jinqiu Fu, Fujun Li, Kai Xia, Shengchun Li, Ling Chang, Ralph Bock, Jiang Zhang

引用次数: 0