CreTPS3a启动子的单ltr插入增强了γ -萜烯的生物合成,并影响了柑橘类水果中消费者对柑橘类香气的偏好。

IF 24.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Huan Wen, Yuan Liu, Zhehui Hu, Dongxuan Wu, Lingling Shui, Zhipeng Zhao, Gu Li, Guixiang Chen, Jiajing Chen, Xiao Liu, Xiaolin Chen, Cecilia Hong Deng, Haipeng Zhang, Xinxin He, Xinxin Zhang, Xiuxin Deng, Andan Zhu, Juan Xu
{"title":"CreTPS3a启动子的单ltr插入增强了γ -萜烯的生物合成,并影响了柑橘类水果中消费者对柑橘类香气的偏好。","authors":"Huan Wen, Yuan Liu, Zhehui Hu, Dongxuan Wu, Lingling Shui, Zhipeng Zhao, Gu Li, Guixiang Chen, Jiajing Chen, Xiao Liu, Xiaolin Chen, Cecilia Hong Deng, Haipeng Zhang, Xinxin He, Xinxin Zhang, Xiuxin Deng, Andan Zhu, Juan Xu","doi":"10.1016/j.molp.2025.09.007","DOIUrl":null,"url":null,"abstract":"<p><p>Aroma differentiation is a key trait distinguishing citrus and other horticultural crops from staple crops. However, the mechanisms and sensory features by which distinctive and various citrus-like aroma in citrus remain poorly understood. In this study, we demonstrated that γ-terpinene determines tangerine-like aroma, affects consumer preference, and has pest-repellent properties. Both forward and reverse genetic analyses indicated the pivotal role of CreTPS3a in γ-terpinene biosynthesis. Additionally, we identified a solo-long terminal repeat (solo-LTR) insertion upstream of the CreTPS3a promoter in MD1-type domesticated mandarins. The transcription factor CreARF2 specifically bind to the solo-LTR, positively regulate CreTPS3a expression and γ-terpinene accumulation. Notably, this mechanism might associate with the geographical distribution patterns of tangerine germplasms. Integrating sensory evaluation with insect behavioral assays, we identified a γ-terpinene sensory threshold of around 50 μg/g that optimally balances pest-repellent properties with consumer preference. Collectively, these findings revealed the molecular mechanisms underlying tangerine-like aroma production, illustrated the complex interactions among citrus plants, human beings and insects, and open new possibilities for developing innovative eco-friendly strategies that may enhance fruit aroma and strengthen plant defense against pests simultaneously.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":24.1000,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A solo-LTR insertion in the CreTPS3a promoter enhances γ -terpinene biosynthesis and affects consumer preference for tangerine-like aroma in citrus fruits.\",\"authors\":\"Huan Wen, Yuan Liu, Zhehui Hu, Dongxuan Wu, Lingling Shui, Zhipeng Zhao, Gu Li, Guixiang Chen, Jiajing Chen, Xiao Liu, Xiaolin Chen, Cecilia Hong Deng, Haipeng Zhang, Xinxin He, Xinxin Zhang, Xiuxin Deng, Andan Zhu, Juan Xu\",\"doi\":\"10.1016/j.molp.2025.09.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Aroma differentiation is a key trait distinguishing citrus and other horticultural crops from staple crops. However, the mechanisms and sensory features by which distinctive and various citrus-like aroma in citrus remain poorly understood. In this study, we demonstrated that γ-terpinene determines tangerine-like aroma, affects consumer preference, and has pest-repellent properties. Both forward and reverse genetic analyses indicated the pivotal role of CreTPS3a in γ-terpinene biosynthesis. Additionally, we identified a solo-long terminal repeat (solo-LTR) insertion upstream of the CreTPS3a promoter in MD1-type domesticated mandarins. The transcription factor CreARF2 specifically bind to the solo-LTR, positively regulate CreTPS3a expression and γ-terpinene accumulation. Notably, this mechanism might associate with the geographical distribution patterns of tangerine germplasms. Integrating sensory evaluation with insect behavioral assays, we identified a γ-terpinene sensory threshold of around 50 μg/g that optimally balances pest-repellent properties with consumer preference. Collectively, these findings revealed the molecular mechanisms underlying tangerine-like aroma production, illustrated the complex interactions among citrus plants, human beings and insects, and open new possibilities for developing innovative eco-friendly strategies that may enhance fruit aroma and strengthen plant defense against pests simultaneously.</p>\",\"PeriodicalId\":19012,\"journal\":{\"name\":\"Molecular Plant\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":24.1000,\"publicationDate\":\"2025-09-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular Plant\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.molp.2025.09.007\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Plant","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.molp.2025.09.007","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

香气分化是柑橘和其他园艺作物区别于主要作物的一个关键特征。然而,人们对柑橘类香气的机制和感官特征仍知之甚少。在本研究中,我们证明了γ-萜烯决定了柑橘类香气,影响消费者偏好,并具有驱虫特性。正向和反向遗传分析均表明CreTPS3a在γ-萜烯生物合成中起关键作用。此外,我们在md1型驯化柑橘的CreTPS3a启动子上游发现了一个solo-long终端重复(solo-LTR)插入。转录因子CreARF2特异性结合solo-LTR,正向调节CreTPS3a的表达和γ-萜烯的积累。值得注意的是,这一机制可能与柑桔种质资源的地理分布模式有关。综合感官评估和昆虫行为分析,我们确定了γ-萜烯的感觉阈值约为50 μg/g,可以最佳地平衡驱虫性能和消费者偏好。总的来说,这些发现揭示了柑橘类香气产生的分子机制,揭示了柑橘植物、人类和昆虫之间复杂的相互作用,并为开发创新的生态策略开辟了新的可能性,这些策略可以同时增强果实香气和增强植物对害虫的防御。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A solo-LTR insertion in the CreTPS3a promoter enhances γ -terpinene biosynthesis and affects consumer preference for tangerine-like aroma in citrus fruits.

Aroma differentiation is a key trait distinguishing citrus and other horticultural crops from staple crops. However, the mechanisms and sensory features by which distinctive and various citrus-like aroma in citrus remain poorly understood. In this study, we demonstrated that γ-terpinene determines tangerine-like aroma, affects consumer preference, and has pest-repellent properties. Both forward and reverse genetic analyses indicated the pivotal role of CreTPS3a in γ-terpinene biosynthesis. Additionally, we identified a solo-long terminal repeat (solo-LTR) insertion upstream of the CreTPS3a promoter in MD1-type domesticated mandarins. The transcription factor CreARF2 specifically bind to the solo-LTR, positively regulate CreTPS3a expression and γ-terpinene accumulation. Notably, this mechanism might associate with the geographical distribution patterns of tangerine germplasms. Integrating sensory evaluation with insect behavioral assays, we identified a γ-terpinene sensory threshold of around 50 μg/g that optimally balances pest-repellent properties with consumer preference. Collectively, these findings revealed the molecular mechanisms underlying tangerine-like aroma production, illustrated the complex interactions among citrus plants, human beings and insects, and open new possibilities for developing innovative eco-friendly strategies that may enhance fruit aroma and strengthen plant defense against pests simultaneously.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信