Molecular Plant最新文献

{"title":"Chemotaxis-driven crop engineering for smart microbiomes.","authors":"Peiyun Ji, Ertao Wang, Yang Bai, Zhiyuan Yin, Daolong Dou","doi":"10.1016/j.molp.2025.07.021","DOIUrl":"10.1016/j.molp.2025.07.021","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1417-1420"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144775861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HDT2-mediated lysine deacetylation promotes phytochrome A degradation during photomorphogenesis in Arabidopsis.","authors":"Feng Zheng, Wenli Ou, Ling Deng, Yahan Wang, Hangcong Chen, Yiting Chen, Tao Peng, Yongyi Yang, Jaime A Teixeira da Silva, Xuncheng Liu","doi":"10.1016/j.molp.2025.08.002","DOIUrl":"10.1016/j.molp.2025.08.002","url":null,"abstract":"<p><p>The shift from skotomorphogenesis to photomorphogenesis, a developmental transition in seed plants, involves dramatic proteomic changes. Lysine acetylation (Lys-Ac) is an evolutionarily conserved and recognized post-translational modification that plays a crucial role in plant development. However, its role in seedling deetiolation remains unclear. In this study, we conducted a comparative lysine acetylomic analysis of etiolated Arabidopsis seedlings before and after red (R) light irradiation, uncovering that exposure to R light mainly led to protein lysine deacetylation during seedling deetiolation. Phytochrome A (phyA), a unique far-red (FR) light photoreceptor, was deacetylated at lysine 65 (K65) when etiolated seedlings were moved to light. This residue is a critical ubiquitination site that regulates phyA stability. Moreover, K65 deacetylation facilitates phyA ubiquitination and 26S proteasome-mediated degradation, and is required for the function of phyA in FR light signaling and seedling photomorphogenesis. Furthermore, we identified a plant-specific lysine deacetylase HDT2 that interacts with and deacetylates phyA in the nucleus to promote its ubiquitination and degradation during seedling deetiolation. Genetic analysis revealed that HDT2 is critical for phyA-mediated photomorphogenic growth. Taken together, these findings reveal that lysine deacetylation of phyA by HDT2 plays a crucial role in modulating phyA turnover in response to light, suggesting that Lys-Ac might be central to the reprogramming of plants for photomorphogenic growth.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1567-1586"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatin remodeling in plants: Complex composition, mechanistic diversity, and biological functions.","authors":"Yisui Huang, Jing Guo, Xin-Jian He, Chenlong Li","doi":"10.1016/j.molp.2025.08.004","DOIUrl":"10.1016/j.molp.2025.08.004","url":null,"abstract":"<p><p>Chromatin remodeling complexes serve as crucial regulators of chromatin structure in eukaryotes, governing the transcription, DNA repair, and genome stability. Compared with chromatin remodelers in yeast and animals, plant chromatin remodelers exhibit both conserved and lineage-specific features, which facilitate unique adaptive responses. Cutting-edge approaches in biochemistry, epigenomics, and proteomics are revealing unprecedented insights into plant chromatin remodeling mechanisms, and genetic studies continue to demonstrate their essential roles in maintaining chromatin state homeostasis during plant growth and stress adaptation. This review synthesizes current understanding of plant chromatin remodeling complexes, with particular focuses on their specialized subunit compositions, mechanistic diversity, and integrative roles in epigenetic regulation. Furthermore, we highlight how these complexes interact with histone modifications, DNA methylation pathways, and transcription factor networks to orchestrate plant development and stress responses.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1436-1457"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordinated regulation of RNA metabolism and silencing by ABA signaling in plant abiotic stress responses.","authors":"Brian D Gregory","doi":"10.1016/j.molp.2025.07.007","DOIUrl":"10.1016/j.molp.2025.07.007","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1424-1426"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolutionary rise of oxygen sensing in land plants.","authors":"Daniel J Gibbs, Lina Zhou","doi":"10.1016/j.molp.2025.07.003","DOIUrl":"10.1016/j.molp.2025.07.003","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1421-1423"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel regulatory module coordinates endosperm starch synthesis, grain development, and yield potential in cereals.","authors":"Bin Ma, Xiubiao Cao, Qi-Qi Zhang, Changquan Zhang, Qiaoquan Liu","doi":"10.1016/j.molp.2025.07.020","DOIUrl":"10.1016/j.molp.2025.07.020","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1433-1435"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dampened nuclear localization of SnRK1 by brassinosteroid signaling inhibits stomatal development in Arabidopsis.","authors":"Lianmei Yao, Shurui Liu, Wen Shi, Yuxin Gan, Min Fan, Filip Rolland, Ming-Yi Bai, Chao Han","doi":"10.1016/j.molp.2025.07.013","DOIUrl":"10.1016/j.molp.2025.07.013","url":null,"abstract":"<p><p>The balance between stem cell division and differentiation is crucial for flexible organ development. In Arabidopsis leaves, the fate of meristemoids, which exhibit stem cell characteristics, is tightly regulated by multiple intrinsic developmental signals and environmental factors. KIN10, the catalytic subunit of the sucrose non-fermenting 1-related protein kinase 1 (SnRK1) complex, has been shown to preferentially localize in the nucleus of meristemoids, where it phosphorylates and stabilizes the SPEECHLESS transcription factor, thereby promoting stomatal development. However, the regulatory mechanism governing the nuclear localization of KIN10 in meristemoids remains unclear. Here, we demonstrate that brassinosteroid (BR) inhibits KIN10's nuclear localization by modulating KINβ2 through BR-INSENSITIVE2 (BIN2)-mediated phosphorylation. In meristemoids, KIN10 is predominantly nuclear, while KINβ2 is mainly cytosolic. Interfering with the nuclear localization of KIN10 or enhancing the membrane association of KINβ2 impairs stomatal development and leads to excessive epidermal cell proliferation. Cell biology and biochemical analyses reveal that BR signaling could inhibit KIN10 nuclear localization by enhancing KINβ2 membrane association, while BIN2 interacts with and phosphorylates KINβ2 to reduce its membrane association and its interaction with KIN10. Taken together, these findings suggest that the precise regulation of the subcellular localization of the SnRK1 complex, at least in part by BR signaling, is critical for meristemoid differentiation and stomatal development.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1490-1504"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative splicing of OsNPR3 promoted by the bacterial TAL effectors-targeted splicing regulator OsRBP11 antagonizes OsNPR1 function and enhances disease susceptibility in rice.","authors":"Xiaochen Chen, Xiaohui Yao, Fang Yan, Shaofang Li, Zuo-Dong Wang, Fu-You Yin, Miao Zhou, Zhen Wang, Lina Qin, Baoguo Zhao, Kai Lu, Liyuan Zhang, Xiaoxu Li, Xiuyan Mu, Yu Zhang, Teng Lu, Jin-Biao Ma, Yi-Kun Zhao, Dewen Lin, Maoling Wang, Qizhen Li, Shuo Qi, Juying Long, Bixin Bai, Jing-Yu Ma, Yanzhi Liu, Yaping Feng, Xue-Bao Yang, Jianhua Zhang, Yuheng Xu, Lei Chen, Shenshen Zou, Xinhua Ding, Mei-Xiang Zhang, Dong-Lei Yang, Zaiquan Cheng, Huanbin Zhou, Hansong Dong","doi":"10.1016/j.molp.2025.07.015","DOIUrl":"10.1016/j.molp.2025.07.015","url":null,"abstract":"<p><p>Plant proteins that belong to the nonexpressor of pathogenesis-related (NPR) gene family are paralogous receptors of the plant defense hormone salicylic acid and essential regulators of hormone-dependent plant immunity against diseases caused by various pathogens. Previous studies have established NPR1 and NPR3 as a transcriptional activator and a transcriptional repressor, respectively, of defense-gene expression to promote and inhibit broad-spectrum resistance against different strains of pathogens. However, the regulatory mechanism that underlies the opposing roles of NPR1 and NPR3 in defense-gene activation remains unclear. Here, we report that a rice transcript splicing factor, Oryza sativa RNA-binding protein 11 (OsRBP11), promotes alternative splicing of OsNPR3 to modulate the defense function of OsNPR1 in rice plants infected by Xanthomonas oryzae pathovars, which are important bacterial pathogens of rice. We discovered that 11 transcription activator-like effectors identified in representative bacterial strains activate OsRBP11 expression. The OsRBP11 protein, in turn, facilitates alternative splicing of the OsNPR3 mRNA precursor, leading to the production of truncated OsNPR3 protein variants. The OsNPR3 variants exacerbate bacterial diseases by sequestering OsNPR1 from defense-gene activation. By contrast, both artificial and natural variations in OsRBP11 prevent the alternative splicing of OsNPR3, restore the defense function of OsNPR1, and enhance rice resistance to different bacterial strains. These findings not only reveal a novel regulatory pathway exploited by bacterial pathogens to facilitate their pathogenicity and subvert plant defense but also provide a genetic basis for biotechnological strategies aimed at developing broad-spectrum resistance in crops.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1505-1525"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural insights into ABA receptor agonists reveal critical features to optimize and design a broad-spectrum ABA signaling activator.","authors":"Mar Bono, Cristian Mayordomo, Alberto Coego, Jonatan Illescas-Miranda, Maria Rivera-Moreno, Lourdes Infantes, Pablo López-Carracedo, Mayra Sanchez-Olvera, Constanza Martin-Vasquez, Gaston A Pizzio, Javier Merino, Javier Forment, Ebe Merilo, Juan Carlos Estevez, Armando Albert, Pedro L Rodriguez","doi":"10.1016/j.molp.2025.07.014","DOIUrl":"10.1016/j.molp.2025.07.014","url":null,"abstract":"<p><p>Crop yield is at increasing risk due to water scarcity and climate change. Agrochemicals can activate hormone receptors to regulate transpiration and modulate transcription and address water deficits. Structure-guided optimization of multiple abscisic acid (ABA) receptor-agonist interactions is necessary to activate the entire PYRABACTIN RESISTANCE 1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) receptor family. The new agonist iCB, produced through scaffold-merging led by X-ray structure, activates subfamilies II and III at low-nM concentrations and subfamily I receptors at higher-nM concentrations. Structural analysis of opabactin and iCB ternary complexes reveals selectivity-determining residues, making the PYL1/PYL4/PYL8 subfamilies sensitive to specific agonists and highlighting the differential sensitivity of receptor subfamilies to agonists across plant species. iCB may activate most eudicots' PYL8-like receptors, in contrast to opabactin, due to limited steric constraints. This enables iCB to activate PYL8-like receptors with a bulkier Leu residue in the 3' tunnel, such as AtPYL8, SlPYL8, and VviPYL8. In contrast, opabactin activation is limited to receptors with Val at this position, for example, TaPYL8. Therefore, iCB extends its action to more ABA receptors than CB, iSB09, and opabactin, exhibits higher affinity than ABA for dimeric receptors, and can protect tomato plants against drought. In addition to regulating stomatal conductance and lowering water consumption, iCB protects photosystem II and improves photosynthesis following prolonged water deficit. Moreover, iCB induces an ABA-like transcriptional response, upregulates the osmolyte synthesis, and can be hyperpotentiated when combined with the expression of a customized receptor. Our results provide structural insights for optimizing agonist design and aiding plants in managing water deficits.</p>","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1526-1548"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two complete telomere-to-telomere genome assemblies of Medicago reveal the landscape and evolution of its centromeres.","authors":"Lisha Shen, Congyang Yi, Yang Liu, Fangpu Han, Jian Feng","doi":"10.1016/j.molp.2025.07.016","DOIUrl":"10.1016/j.molp.2025.07.016","url":null,"abstract":"","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1409-1412"},"PeriodicalIF":24.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}