Molecular Plant最新文献_第5页

ITPK1 is an InsP₆/ADP phosphotransferase that controls phosphate signaling in Arabidopsis. ITPK1是一种InsP6/ADP磷酸转移酶，控制拟南芥中的磷酸盐信号转导。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-03 DOI: 10.1016/j.molp.2024.05.004

Esther Riemer, Danye Qiu, Debabrata Laha, Robert K Harmel, Philipp Gaugler, Verena Gaugler, Michael Frei, Mohammad-Reza Hajirezaei, Nargis Parvin Laha, Lukas Krusenbaum, Robin Schneider, Adolfo Saiardi, Dorothea Fiedler, Henning J Jessen, Gabriel Schaaf, Ricardo F H Giehl

引用次数: 0

Two MADS-box proteins, AGL9 and AGL15, recruit the FIS-PRC2 complex to trigger the phase transition from endosperm proliferation to embryo development in Arabidopsis. 两个 MADS-box 蛋白 AGL9 和 AGL15 招募 FIS-PRC2 复合物，触发拟南芥从胚乳增殖到胚胎发育的阶段转换。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-01 DOI: 10.1016/j.molp.2024.05.011

Shen Zhang, Devasantosh Mohanty, Adnan Muzaffar, Min Ni

{"title":"Two MADS-box proteins, AGL9 and AGL15, recruit the FIS-PRC2 complex to trigger the phase transition from endosperm proliferation to embryo development in Arabidopsis.","authors":"Shen Zhang, Devasantosh Mohanty, Adnan Muzaffar, Min Ni","doi":"10.1016/j.molp.2024.05.011","DOIUrl":"10.1016/j.molp.2024.05.011","url":null,"abstract":"Spatiotemporal regulation of gene expression by polycomb repressive complex 2 (PRC2) is critical for animal and plant development. The Arabidopsis fertilization independent seed (FIS)-PRC2 complex functions specifically during plant reproduction from gametogenesis to seed development. After a double fertilization event, triploid endosperm proliferates early, followed by the growth of a diploid embryo, which replaces the endosperm in Arabidopsis and many dicots. Key genes critical for endosperm proliferation such as IKU2 and MINI3 are activated after fertilization. Here we report that two MADS-box AGAMOUS-LIKE (AGL) proteins associate with the key endosperm proliferation loci and recruit the FIS-PRC2 repressive complex at 4-5 days after pollination (DAP). Interestingly, AGL9 and AGL15 only accumulate toward the end of endosperm proliferation at 4-5 DAP and promote the deposition of H3K27me3 marks at key endosperm proliferation loci. Disruption of AGL9 and AGL15 or overexpression of AGL9 or AGL15 significantly influence endosperm proliferation and cellularization. Genome-wide analysis with cleavage Under Targets and tagmentation (CUT&Tag) sequencing and RNA sequencing revealed the landscape of endosperm H3K27me3 marks and gene expression profiles in Col-0 and agl9 agl15. CUT&Tag qPCR also demonstrated the occupancy of the two MADS-box proteins and FIS-PRC2 on a few representative target loci. Our studies suggest that MADS-box proteins could potentially recruit PRC2 to regulate many other developmental processes in plants or even in fungi and animals.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The biosynthetic pathway of the hallucinogen mescaline and its heterologous reconstruction. 致幻剂麦司卡林的生物合成途径及其异源重建。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-03 DOI: 10.1016/j.molp.2024.05.012

Paula Berman, Luis Alejandro de Haro, Ana-Rita Cavaco, Sayantan Panda, Younghui Dong, Nikolay Kuzmich, Gabriel Lichtenstein, Yoav Peleg, Hila Harat, Adam Jozwiak, Jianghua Cai, Uwe Heinig, Sagit Meir, Ilana Rogachev, Asaph Aharoni

{"title":"The biosynthetic pathway of the hallucinogen mescaline and its heterologous reconstruction.","authors":"Paula Berman, Luis Alejandro de Haro, Ana-Rita Cavaco, Sayantan Panda, Younghui Dong, Nikolay Kuzmich, Gabriel Lichtenstein, Yoav Peleg, Hila Harat, Adam Jozwiak, Jianghua Cai, Uwe Heinig, Sagit Meir, Ilana Rogachev, Asaph Aharoni","doi":"10.1016/j.molp.2024.05.012","DOIUrl":"10.1016/j.molp.2024.05.012","url":null,"abstract":"Mescaline, among the earliest identified natural hallucinogens, holds great potential in psychotherapy treatment. Nonetheless, despite the existence of a postulated biosynthetic pathway for more than half a century, the specific enzymes involved in this process are yet to be identified. In this study, we investigated the cactus Lophophora williamsii (Peyote), the largest known natural producer of the phenethylamine mescaline. We employed a multi-faceted approach, combining de novo whole-genome and transcriptome sequencing with comprehensive chemical profiling, enzymatic assays, molecular modeling, and pathway engineering for pathway elucidation. We identified four groups of enzymes responsible for the six catalytic steps in the mescaline biosynthetic pathway, and an N-methyltransferase enzyme that N-methylates all phenethylamine intermediates, likely modulating mescaline levels in Peyote. Finally, we reconstructed the mescaline biosynthetic pathway in both Nicotiana benthamiana plants and yeast cells, providing novel insights into several challenges hindering complete heterologous mescaline production. Taken together, our study opens up avenues for exploration of sustainable production approaches and responsible utilization of mescaline, safeguarding this valuable natural resource for future generations.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141248118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Doubled haploid technology and synthetic apomixis: Recent advances and applications in future crop breeding. 双单倍体技术与合成 Apomixis：未来作物育种的最新进展和应用》。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-13 DOI: 10.1016/j.molp.2024.06.005

Yanzhi Qu, Alisdair R Fernie, Jie Liu, Jianbing Yan

引用次数: 0

Stabilization or degradation? Post-translational modifications of JAZ proteins in plants. 稳定还是降解？植物中 JAZ 蛋白的翻译后修饰。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-19 DOI: 10.1016/j.molp.2024.06.010

Xiaoyan Xu, Jianping Hu, Zheng Yuan

引用次数: 0

A k-mer-based pangenome approach for cataloging seed-storage-protein genes in wheat to facilitate genotype-to-phenotype prediction and improvement of end-use quality. 基于k-mer的pangenome方法，对小麦种子贮藏蛋白基因进行编目，以促进基因型到表型的预测和最终使用质量的改善。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-05-24 DOI: 10.1016/j.molp.2024.05.006

Zhaoheng Zhang, Dan Liu, Binyong Li, Wenxi Wang, Jize Zhang, Mingming Xin, Zhaorong Hu, Jie Liu, Jinkun Du, Huiru Peng, Chenyang Hao, Xueyong Zhang, Zhongfu Ni, Qixin Sun, Weilong Guo, Yingyin Yao

{"title":"A k-mer-based pangenome approach for cataloging seed-storage-protein genes in wheat to facilitate genotype-to-phenotype prediction and improvement of end-use quality.","authors":"Zhaoheng Zhang, Dan Liu, Binyong Li, Wenxi Wang, Jize Zhang, Mingming Xin, Zhaorong Hu, Jie Liu, Jinkun Du, Huiru Peng, Chenyang Hao, Xueyong Zhang, Zhongfu Ni, Qixin Sun, Weilong Guo, Yingyin Yao","doi":"10.1016/j.molp.2024.05.006","DOIUrl":"10.1016/j.molp.2024.05.006","url":null,"abstract":"Wheat is a staple food for more than 35% of the world's population, with wheat flour used to make hundreds of baked goods. Superior end-use quality is a major breeding target; however, improving it is especially time-consuming and expensive. Furthermore, genes encoding seed-storage proteins (SSPs) form multi-gene families and are repetitive, with gaps commonplace in several genome assemblies. To overcome these barriers and efficiently identify superior wheat SSP alleles, we developed \"PanSK\" (Pan-SSP k-mer) for genotype-to-phenotype prediction based on an SSP-based pangenome resource. PanSK uses 29-mer sequences that represent each SSP gene at the pangenomic level to reveal untapped diversity across landraces and modern cultivars. Genome-wide association studies with k-mers identified 23 SSP genes associated with end-use quality that represent novel targets for improvement. We evaluated the effect of rye secalin genes on end-use quality and found that removal of ω-secalins from 1BL/1RS wheat translocation lines is associated with enhanced end-use quality. Finally, using machine-learning-based prediction inspired by PanSK, we predicted the quality phenotypes with high accuracy from genotypes alone. This study provides an effective approach for genome design based on SSP genes, enabling the breeding of wheat varieties with superior processing capabilities and improved end-use quality.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progressive meristem and single-cell transcriptomes reveal the regulatory mechanisms underlying maize inflorescence development and sex differentiation. 渐进分生组织和单细胞转录组揭示了玉米花序发育和性别分化的调控机制。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-13 DOI: 10.1016/j.molp.2024.06.007

Yonghao Sun, Liang Dong, Lu Kang, Wanshun Zhong, David Jackson, Fang Yang

{"title":"Progressive meristem and single-cell transcriptomes reveal the regulatory mechanisms underlying maize inflorescence development and sex differentiation.","authors":"Yonghao Sun, Liang Dong, Lu Kang, Wanshun Zhong, David Jackson, Fang Yang","doi":"10.1016/j.molp.2024.06.007","DOIUrl":"10.1016/j.molp.2024.06.007","url":null,"abstract":"Maize develops separate ear and tassel inflorescences with initially similar morphology but ultimately different architecture and sexuality. The detailed regulatory mechanisms underlying these changes still remain largely unclear. In this study, through analyzing the time-course meristem transcriptomes and floret single-cell transcriptomes of ear and tassel, we revealed the regulatory dynamics and pathways underlying inflorescence development and sex differentiation. We identified 16 diverse gene clusters with differential spatiotemporal expression patterns and revealed biased regulation of redox, programmed cell death, and hormone signals during meristem differentiation between ear and tassel. Notably, based on their dynamic expression patterns, we revealed the roles of two RNA-binding proteins in regulating inflorescence meristem activity and axillary meristem formation. Moreover, using the transcriptional profiles of 53 910 single cells, we uncovered the cellular heterogeneity between ear and tassel florets. We found that multiple signals associated with either enhanced cell death or reduced growth are responsible for tassel pistil suppression, while part of the gibberellic acid signal may act non-cell-autonomously to regulate ear stamen arrest during sex differentiation. We further showed that the pistil-protection gene SILKLESS 1 (SK1) functions antagonistically to the known pistil-suppression genes through regulating common molecular pathways, and constructed a regulatory network for pistil-fate determination. Collectively, our study provides a deep understanding of the regulatory mechanisms underlying inflorescence development and sex differentiation in maize, laying the foundation for identifying new regulators and pathways for maize hybrid breeding and improvement.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141321277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant triterpenoid saponins function as susceptibility factors to promote the pathogenicity of Botrytis cinerea. 植物三萜皂苷作为易感因子可促进灰霉病菌的致病性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-05-27 DOI: 10.1016/j.molp.2024.05.008

Francisco J Escaray, Amelia Felipo-Benavent, Cristian J Antonelli, Begoña Balaguer, Maria Pilar Lopez-Gresa, Pablo Vera

{"title":"Plant triterpenoid saponins function as susceptibility factors to promote the pathogenicity of Botrytis cinerea.","authors":"Francisco J Escaray, Amelia Felipo-Benavent, Cristian J Antonelli, Begoña Balaguer, Maria Pilar Lopez-Gresa, Pablo Vera","doi":"10.1016/j.molp.2024.05.008","DOIUrl":"10.1016/j.molp.2024.05.008","url":null,"abstract":"The gray mold fungus Botrytis cinerea is a necrotrophic pathogen that causes diseases in hundreds of plant species, including high-value crops. Its polyxenous nature and pathogenic success are due to its ability to perceive host signals in its favor. In this study, we found that laticifer cells of Euphorbia lathyris are a source of susceptibility factors required by B. cinerea to cause disease. Consequently, poor-in-latex (pil) mutants, which lack laticifer cells, show full resistance to this pathogen, whereas lot-of-latex mutants, which produce more laticifer cells, are hypersusceptible. These S factors are triterpenoid saponins, which are widely distributed natural products of vast structural diversity. The downregulation of laticifer-specific oxydosqualene cyclase genes, which encode the first committed step enzymes for triterpene and, therefore, saponin biosynthesis, conferred disease resistance to B. cinerea. Likewise, the Medicago truncatula lha-1 mutant, compromised in triterpenoid saponin biosynthesis, showed enhanced resistance. Interestingly, the application of different purified triterpenoid saponins pharmacologically complemented the disease-resistant phenotype of pil and hla-1 mutants and enhanced disease susceptibility in different plant species. We found that triterpenoid saponins function as plant cues that signal transcriptional reprogramming in B. cinerea, leading to a change in its growth habit and infection strategy, culminating in the abundant formation of infection cushions, the multicellular appressoria apparatus dedicated to plant penetration and biomass destruction in B. cinerea. Taken together, these results provide an explanation for how plant triterpenoid saponins function as disease susceptibility factors to promote B. cinerea pathogenicity.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141162267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear transport receptors underpin plastidial retrograde signaling. 核转运受体支撑着质体逆行信号转导

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-06-07 DOI: 10.1016/j.molp.2024.06.003

Min Jia, Yangnan Gu

引用次数: 0

Non-transcriptional regulatory activity of SMAX1 and SMXL2 mediates karrikin-regulated seedling response to red light in Arabidopsis. SMAX1和SMXL2的非转录调控活性介导拟南芥幼苗对红光的反应。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-07-01 Epub Date: 2024-05-27 DOI: 10.1016/j.molp.2024.05.007

Wenwen Chang, Qiao Qiao, Qingtian Li, Xin Li, Yanyan Li, Xiahe Huang, Yingchun Wang, Jiayang Li, Bing Wang, Lei Wang

{"title":"Non-transcriptional regulatory activity of SMAX1 and SMXL2 mediates karrikin-regulated seedling response to red light in Arabidopsis.","authors":"Wenwen Chang, Qiao Qiao, Qingtian Li, Xin Li, Yanyan Li, Xiahe Huang, Yingchun Wang, Jiayang Li, Bing Wang, Lei Wang","doi":"10.1016/j.molp.2024.05.007","DOIUrl":"10.1016/j.molp.2024.05.007","url":null,"abstract":"Karrikins and strigolactones govern plant development and environmental responses through closely related signaling pathways. The transcriptional repressor proteins SUPPRESSOR OF MAX2 1 (SMAX1), SMAX1-like2 (SMXL2), and D53-like SMXLs mediate karrikin and strigolactone signaling by directly binding downstream genes or by inhibiting the activities of transcription factors. In this study, we characterized the non-transcriptional regulatory activities of SMXL proteins in Arabidopsis. We discovered that SMAX1 and SMXL2 with mutations in their ethylene-response factor-associated amphiphilic repression (EAR) motif had undetectable or weak transcriptional repression activities but still partially rescued the hypocotyl elongation defects and fully reversed the cotyledon epinasty defects of the smax1 smxl2 mutant. SMAX1 and SMXL2 directly interact with PHYTOCHROME INTERACTION FACTOR 4 (PIF4) and PIF5 to enhance their protein stability by interacting with phytochrome B (phyB) and suppressing the association of phyB with PIF4 and PIF5. The karrikin-responsive genes were then identified by treatment with GR24ent-5DS, a GR24 analog showing karrikin activity. Interestingly, INDOLE-3-ACETIC ACID INDUCIBLE 29 (IAA29) expression was repressed by GR24ent-5DS treatment in a PIF4- and PIF5-dependent and EAR-independent manner, whereas KARRIKIN UPREGULATED F-BOX 1 (KUF1) expression was induced in a PIF4- and PIF5-independent and EAR-dependent manner. Furthermore, the non-transcriptional regulatory activity of SMAX1, which is independent of the EAR motif, had a global effect on gene expression. Taken together, these results indicate that non-transcriptional regulatory activities of SMAX1 and SMXL2 mediate karrikin-regulated seedling response to red light.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":null,"pages":null},"PeriodicalIF":17.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141162263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0