Molecular Plant最新文献_第5页

RNA warfare: Long non-coding RNAs in plant-microbe cross-kingdom regulation. RNA之战：植物-微生物跨界调控中的长链非编码RNA。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-07-08 DOI: 10.1016/j.molp.2026.07.003

Rui-Rui He, Jie Jiang, Yang Yu, Yue-Qin Chen

引用次数: 0

Fungal endophyte evasion of plant immune system for mutual benefit: Now you see me, now you don't. 真菌内生菌逃避植物免疫系统互惠互利：你现在看到我，你现在没有。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-07-15 DOI: 10.1016/j.molp.2026.07.010

Antonio Molina, Miguel Ángel Torres, Lucía Jordá

引用次数: 0

Building a stem cell niche from the outside in: Spatial architecture as a code for regeneration. 从外到内构建干细胞生态位：作为再生代码的空间建筑。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-07-08 DOI: 10.1016/j.molp.2026.07.005

Hatsune Morinaka

引用次数: 0

A sucrose-jasmonic acid feedback loop drives stem cell senescence in Arabidopsis. 蔗糖和茉莉酸正反馈回路控制拟南芥干细胞衰老。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-06-17 DOI: 10.1016/j.molp.2026.06.004

Yan-Die Chen, Rui Zhang, Jing Yang, Zhong Zhao

{"title":"A sucrose-jasmonic acid feedback loop drives stem cell senescence in Arabidopsis.","authors":"Yan-Die Chen, Rui Zhang, Jing Yang, Zhong Zhao","doi":"10.1016/j.molp.2026.06.004","DOIUrl":"10.1016/j.molp.2026.06.004","url":null,"abstract":"The lifespan of annual plants is tightly linked to stem cell senescence and the cessation of proliferative activity in the inflorescence meristem (IM). A \"senescence factor\" originating in developing fruits has long been proposed to trigger proliferative arrest in the IM. Here, we show that jasmonic acid (JA) accumulates in the aging IM, where the JA-responsive transcription factor MYC2 directly represses WUSCHEL (WUS) expression and cytokinin accumulation. We demonstrate that sucrose originating in the siliques triggers local JA biosynthesis in the IM and induces stem cell senescence and meristematic arrest. Moreover, expression of SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTER (SWEET) genes increases in the IM at late developmental stages and is directly activated by MYC2. Mobile sucrose and local JA signaling form a positive feedback loop that reinforces stem cell repression in the IM. Our results suggest that sucrose from siliques acts as a senescence factor and, together with the previously described auxin and FRUITFULL/miR172 pathways, defines the timing of IM aging, which has important implications for plant fitness and agricultural productivity.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1803-1818"},"PeriodicalIF":32.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148272226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The domestication-associated WHP10 tandem cluster of amino acid transporter genes enhances whole-plant protein accumulation in maize. 驯化相关的氨基酸转运基因WHP10串联簇促进玉米全株蛋白质积累。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-07-20 DOI: 10.1016/j.molp.2026.07.014

Wenhao Li, Youliang Li, Xiang Lin, Ruilin Chen, Wenjing Li, Chong You, Di Chen, Lulu Gao, Xiaoling Li, Shiqi Luo, Yunlong Li, Yuhua Long, Zhaoxu Hu, Yidong Zhu, Xin Li, Zhigui Bao, Yincong Gu, Shouchuang Wang, Linhan Sun, Wenqin Wang, Haihai Wang, Yongrui Wu, Yongcai Huang

{"title":"The domestication-associated WHP10 tandem cluster of amino acid transporter genes enhances whole-plant protein accumulation in maize.","authors":"Wenhao Li, Youliang Li, Xiang Lin, Ruilin Chen, Wenjing Li, Chong You, Di Chen, Lulu Gao, Xiaoling Li, Shiqi Luo, Yunlong Li, Yuhua Long, Zhaoxu Hu, Yidong Zhu, Xin Li, Zhigui Bao, Yincong Gu, Shouchuang Wang, Linhan Sun, Wenqin Wang, Haihai Wang, Yongrui Wu, Yongcai Huang","doi":"10.1016/j.molp.2026.07.014","DOIUrl":"10.1016/j.molp.2026.07.014","url":null,"abstract":"Improving protein accumulation in maize is essential for sustainable agriculture, yet the regulatory mechanisms governing the intermediate \"flow\" of organic nitrogen remain elusive. Here, we show that the maize stem acts as a regulatory node for nitrogen allocation. By integrating spatial transcriptomics and metabolomics with quantitative genetics, we demonstrate that a transport-oriented stem program orchestrates the high-protein phenotype of the wild maize accession Ames21814. We identified a major locus, Whole-plant High Protein 10 (WHP10), that encodes a tandemly duplicated cluster of amino acid transporter genes. WHP10 exhibits strong vascular-biased expression, driven by promoter divergence that enhances the wild allele's activity. Functional assays and genetic validation support a model in which the WHP10 cluster facilitates the transport of multiple nitrogen-rich amino acids, thereby contributing to vascular-associated amino acid transport and post-uptake organic-nitrogen partitioning. Our findings establish stem flow as a regulatory layer for protein accumulation and identify WHP10 as a high-value target for precision breeding to enhance whole-plant protein accumulation without compromising grain yield.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1911-1931"},"PeriodicalIF":32.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput glycan array screening reveals rhamnogalacturonan-I as a ligand for Arabidopsis leucine-rich repeat receptor kinases involved in plant immunity. 高通量聚糖阵列筛选揭示鼠李糖半乳糖醛酸- 1作为拟南芥富含亮氨酸重复受体激酶参与植物免疫的配体。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-06-17 DOI: 10.1016/j.molp.2026.06.005

Du-Hwa Lee, Colin Ruprecht, Jung-Min Lee, Min-Soo Choi, Tobias Hrovat, Hyeonmin Ryu, Sejin Choi, Geon Heo, Natalie Edelbacher, Balaji Enugutti, Markus Blaukopf, Chung Hyun Cho, Ho-Seok Lee, Youssef Belkhadir, Elwira Smakowska-Luzan, Fabian Pfrengle

{"title":"High-throughput glycan array screening reveals rhamnogalacturonan-I as a ligand for Arabidopsis leucine-rich repeat receptor kinases involved in plant immunity.","authors":"Du-Hwa Lee, Colin Ruprecht, Jung-Min Lee, Min-Soo Choi, Tobias Hrovat, Hyeonmin Ryu, Sejin Choi, Geon Heo, Natalie Edelbacher, Balaji Enugutti, Markus Blaukopf, Chung Hyun Cho, Ho-Seok Lee, Youssef Belkhadir, Elwira Smakowska-Luzan, Fabian Pfrengle","doi":"10.1016/j.molp.2026.06.005","DOIUrl":"10.1016/j.molp.2026.06.005","url":null,"abstract":"The plant cell wall not only serves as a physical barrier against pathogens but, when damaged, also functions as a source of cell wall-derived molecules that play crucial roles in plant immunity as damage-associated molecular patterns. While oligogalacturonides from homogalacturonan are well-studied damage-associated molecular patterns, the immune-signaling potential of other cell wall components remains largely unexplored. Conventional genetic and biochemical approaches aimed at identifying ligand-receptor pairs in plant immunity have been limited by the vast diversity of potential ligand molecules and functional redundancy of putative receptors. In this study, we developed a high-throughput screening pipeline that simultaneously examines multiple interactions between plant cell wall-derived glycans and >350 extracellular domains of receptor kinases and receptor-like proteins in Arabidopsis, resulting in the screening of >40 000 interactions. We discovered a group of leucine-rich repeat receptor kinases named ARMs (AWARENESS of RG-I MAINTENANCES) that interact with rhamnogalacturonan-I (RG-I), a major component of pectin. RG-I treatment induced pattern-triggered immunity responses with distinct kinetics compared to oligogalacturonide responses. We identified RG-I oligosaccharide structures required for interaction with ARM receptors and immune activation and found that ARM receptors function redundantly in plant immunity. Collectively, our work provides a powerful platform for discovering glycan-receptor pairs in plants, facilitating a more comprehensive understanding of cell wall surveillance mechanisms in plant immunity.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1785-1802"},"PeriodicalIF":32.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148272166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of ancestral plant genomes for inter-crop translational research. 用于作物间转化研究的祖先植物基因组重建。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-07-01 DOI: 10.1016/j.molp.2026.06.012

Cléa Siguret, Margaux Olivier, Cécile Huneau, Mamadou Dia Sow, Pierre-Louis Stenger, Christophe Klopp, Marie-Laure Martin, Jean-Philippe Tamby, Serguei Gorbounov, Raphael Flores, Fabrice Legeai, Matéo Boudet, Raffaella Battaglia, Davide Guerra, Peter Civan, Caroline Pont, Anne-Françoise Adam-Blondon, Luigi Cattivelli, Olivier Mathieu, Jerome Salse

{"title":"Reconstruction of ancestral plant genomes for inter-crop translational research.","authors":"Cléa Siguret, Margaux Olivier, Cécile Huneau, Mamadou Dia Sow, Pierre-Louis Stenger, Christophe Klopp, Marie-Laure Martin, Jean-Philippe Tamby, Serguei Gorbounov, Raphael Flores, Fabrice Legeai, Matéo Boudet, Raffaella Battaglia, Davide Guerra, Peter Civan, Caroline Pont, Anne-Françoise Adam-Blondon, Luigi Cattivelli, Olivier Mathieu, Jerome Salse","doi":"10.1016/j.molp.2026.06.012","DOIUrl":"10.1016/j.molp.2026.06.012","url":null,"abstract":"We present Ancestral Genome Reconstruction (AGR), an exploratory framework for the automated inference of \"paleogenomes\" from large-scale comparative datasets. By analyzing 84 extant angiosperm species, we reconstructed 10 key ancestral angiosperm genomes millions of years old. These reconstructed ancestors were instrumental in (1) estimating when angiosperms emerged, when major botanical families originated, and when shared ancestral whole-genome duplication events occurred; and (2) tracing the evolutionary trajectories of ancestral chromosomes and genes, especially those that may have driven the emergence of key life-history traits (e.g., woody vs. herbaceous, aquatic vs. terrestrial, C3 vs. C4, and symbiotic root-nodulating vs. non-nodulating species). We demonstrated that these paleogenomes serve as tractable backbones for inter-crop translational research. Through an open-access web tool, OrthoViewer, we identified orthologs that have retained the same ancestral genomic context, favoring the identification of genes associated with \"phenologs\"- orthologous genes across species driving analogous phenotypes, traits, or processes-exemplified by FUWA for yield components, FLC for flowering time, and DDM1 for DNA methylation. Taken together, this study provides a testable paleogenomic workflow, opening novel avenues for integrating evolutionary genomics data into modern climate-smart crop breeding and supporting the agroecological transition.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1854-1872"},"PeriodicalIF":32.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148369099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From pan-life phase insights to PhaseHub: Analyzing protein condensate complexity. 从泛生命阶段洞察到PhaseHub：分析蛋白质凝析物的复杂性。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-07-29 DOI: 10.1016/j.molp.2026.07.019

Qiyu Liang, Weibo Gao, Yansong Miao

{"title":"From pan-life phase insights to PhaseHub: Analyzing protein condensate complexity.","authors":"Qiyu Liang, Weibo Gao, Yansong Miao","doi":"10.1016/j.molp.2026.07.019","DOIUrl":"10.1016/j.molp.2026.07.019","url":null,"abstract":"Intracellular biomolecular condensation forms multicomponent signaling hubs that regulate development, stress responses, and environmental adaptation. While the molecular grammar encoded within scaffold proteins defines the basal associative features driving condensation, heterotypic condensates are intrinsically dynamic, multicomponent, and far-from-equilibrium systems. Consequently, how condensates organize component composition, stoichiometry, and functional specificity in space and time under physiological conditions remains poorly understood. Addressing this challenge requires integrative frameworks that combine predictive biophysical features with experimental information on protein abundance, interaction networks, subcellular localization, and evolutionary conservation. In this study, we first analyzed phase separation (PS) proteins across the tree of life in 1106 species, revealing a stark contrast in computationally predicted PS propensity between eukaryotes and prokaryotes, with genome size as a key determinant. Through a broad analysis of amino acid homorepeat-containing proteins (HRPs) across all species, we uncovered how PS evolves via a balance between functional condensation and avoidance of harmful, aggregation-prone sequences. We further identified potential signaling hubs and components across kingdoms by integrating PS-positive proteins with experimentally derived abundance and interactome data from four model eukaryotic species. Using Arabidopsis as a model, we dissected the relationships among PS propensity, condensation hub prediction, HRPs, subcellular localization, and structural conservation. Finally, we developed PhaseHub, a user-friendly interface for exploring scaffold-client dynamics, PS components, sequence signatures within each PS protein, and hubs. Collectively, our work provides an evolutionary framework for understanding multicomponent PS hubs by integrating molecular grammar with physiological context, thereby facilitating hypothesis generation and rational design.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1769-1784"},"PeriodicalIF":32.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revolution in plant breeding: From field to factory. 植物育种革命：从田间到工厂。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-07-20 DOI: 10.1016/j.molp.2026.07.017

Junjie Fu, Cong Tan, Xingping Zhang, Liang Li, Zonggeng Li, Jianan Zhang, Junhui Wang, Huan Liu, Qichang Yang, Richard G F Visser, Yunbi Xu

引用次数: 0

Heat-induced methylglyoxal impairs plant thermotolerance by repressing cpHSC70-1-mediated chloroplast protein import via post-translational modification. 热诱导甲基乙二醛通过翻译后修饰抑制cphsc70 -1介导的叶绿体蛋白进口，从而损害植物的耐热性。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-09-07 Epub Date: 2026-06-30 DOI: 10.1016/j.molp.2026.06.010

Feng Ding, Yue-Xin Liu, Shi-Hang Fan, Wei Hua, Ying-Tang Lu, Zheng-Wei Fu

{"title":"Heat-induced methylglyoxal impairs plant thermotolerance by repressing cpHSC70-1-mediated chloroplast protein import via post-translational modification.","authors":"Feng Ding, Yue-Xin Liu, Shi-Hang Fan, Wei Hua, Ying-Tang Lu, Zheng-Wei Fu","doi":"10.1016/j.molp.2026.06.010","DOIUrl":"10.1016/j.molp.2026.06.010","url":null,"abstract":"Global warming threatens agricultural productivity, making it crucial to understand how plants perceive and respond to heat stress. Although various metabolic pathways are known to participate in plant heat responses, the role of metabolites in post-translational regulation under heat stress remains poorly understood. Here, we report that methylglyoxal (MG) functions as a negative modulator of plant thermotolerance. We demonstrate that heat stress induces accumulation of the photorespiratory metabolite 2-phosphoglycolate (2PG), which inhibits the activity of plastid triose phosphate isomerase (pdTPI), thereby increasing MG levels. Through a proteomic approach, we identified the chloroplast chaperone cpHSC70-1 as a primary target of MG. MG modifies cpHSC70-1 at a conserved arginine residue (R373), inhibiting its ATPase activity and consequently impairing chloroplast protein import under heat stress. Genetic evidence confirms that the 2PG-pdTPI module controls MG accumulation and that MG exerts its thermosensitive effect through cpHSC70-1. Our work reveals a photorespiratory metabolite-driven post-translational regulatory pathway, elucidating a novel mechanism for metabolic control of plant thermotolerance.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1837-1853"},"PeriodicalIF":32.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148361472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0