Molecular Plant最新文献_第5页

Phosphorylation/dephosphorylation-mediated regulation of ABI1/2 activity and stability for fine-tuning ABA signaling. 磷酸化/去磷酸化介导的ABI1/2活性调控和ABA信号的稳定性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-13 DOI: 10.1016/j.molp.2025.05.006

Mar Bono, Maria Rivera-Moreno, Armando Albert, Pedro L Rodriguez

引用次数: 0

Antisense-mediated regulation of nitric oxide biosynthesis. 一氧化氮生物合成的反义调控。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-17 DOI: 10.1016/j.molp.2025.03.010

Wil Prall, Martin Crespi

引用次数: 0

Elucidation and de novo reconstitution of glyceollin biosynthesis. 甘油生物合成的阐释和从头重构。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-04-10 DOI: 10.1016/j.molp.2025.04.003

Yunlong Sun, Cong Chen, Chao Lin, Hao Zhang, Jiazhang Lian, Benke Hong

{"title":"Elucidation and de novo reconstitution of glyceollin biosynthesis.","authors":"Yunlong Sun, Cong Chen, Chao Lin, Hao Zhang, Jiazhang Lian, Benke Hong","doi":"10.1016/j.molp.2025.04.003","DOIUrl":"https://doi.org/10.1016/j.molp.2025.04.003","url":null,"abstract":"Glyceollins are phytoalexins, soybean-produced compounds that respond to pathogen invasion, injury, and environmental challenges. While these compounds have diverse bioactivities, their limited accessibility hinders further physiological and biochemical studies. Additionally, the incomplete understanding of glyceollin biosynthesis, particularly cyclization steps, remains a major barrier to sustainable production through synthetic biology. In this study, we uncover the complete biosynthetic pathway of glyceollins through a combinatorial approach involving transient expression in Nicotiana benthamiana, in vitro enzyme characterization, and yeast feeding studies. We identified previously uncharacterized genes encoding reductases for 7,2',4'-trihydroxyisoflavanol biosynthesis and five P450 enzymes that mediate the final oxidative cyclization to produce glyceollins I, II, and III. By de novo reconstruction of the pathway through synthetic biology and metabolic engineering, we successfully produced glyceollins from simple carbon sources in baker's yeast. This work advances the understanding of glyceollin biosynthesis in soybeans, enables sustainable production in microbial hosts, and offers new opportunities for their application in agriculture and biology.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"18 5","pages":"820-832"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-cadmium rice for food safety: OsCS1 is a genetic breakthrough. 低镉大米促进食品安全：OsCS1是一项基因突破。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-25 DOI: 10.1016/j.molp.2025.03.013

Iqra Noor, Hamza Sohail, Biao Jin, Changquan Zhang

引用次数: 0

Two E-clade protein phosphatase 2Cs enhance ABA signaling by dephosphorylating ABI1 in Arabidopsis. 在拟南芥中，两个e枝蛋白磷酸酶2c通过去磷酸化ABI1来增强ABA信号。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-31 DOI: 10.1016/j.molp.2025.03.019

Ya Zhang, Liyuan Han, Junjie Liu, Miao Chang, Chuanling Li, Jian-Xiu Shang, Zhiping Deng, Wenqiang Tang, Yu Sun

{"title":"Two E-clade protein phosphatase 2Cs enhance ABA signaling by dephosphorylating ABI1 in Arabidopsis.","authors":"Ya Zhang, Liyuan Han, Junjie Liu, Miao Chang, Chuanling Li, Jian-Xiu Shang, Zhiping Deng, Wenqiang Tang, Yu Sun","doi":"10.1016/j.molp.2025.03.019","DOIUrl":"10.1016/j.molp.2025.03.019","url":null,"abstract":"ABA INSENSITIVE 1 (ABI1) and ABI2 are co-receptors of the phytohormone abscisic acid (ABA). Studies have demonstrated that phosphorylation of multiple amino acids on ABI1/2 augments their ability to inhibit ABA signaling in planta. However, whether and how the dephosphorylation of ABI1/2 is regulated to enhance plant sensitivity to ABA remain unknown. In this study, we identified two protein phosphatases, designated ABI1-Dephosphorylating E-clade PP2C 1 (ADEP1) and ADEP2, that interact with ABI1/2. Mutants lacking ADEP1, ADEP2, or both (adep1/2) exhibited reduced ABA inhibition of seed germination and root growth, as well as lower levels of ABA-induced stomatal closure. In addition, ABA-induced accumulation of ABI5 protein and expression of downstream target genes are reduced in the adep1/2 mutant compared with the wild type. These findings suggest that ADEP1/2 function as positive regulators of the ABA signaling pathway. Mass spectrometry analysis and two-dimensional electrophoresis identified Ser117 as a major ABA-induced phosphorylation site on the ABI1 protein. ADEP1/2 can dephosphorylate Ser117, leading to destabilization of the ABI1 protein and increased sensitivity of plants to ABA. Moreover, ABA treatment decreases the abundance of ADEP1/2 proteins. In summary, our study reveals two novel regulatory proteins that modulate ABA signaling and provides new insights into the regulatory network that fine-tunes plant ABA responses.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"783-796"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative spatial transcriptomics reveals root dryland adaptation mechanism in rice and HMGB1 as a key regulator. 比较空间转录组学揭示了水稻根系旱地适应机制和HMGB1作为关键调控因子的作用。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-04-06 DOI: 10.1016/j.molp.2025.04.001

Liyuan Zhong, Leping Geng, Yimeng Xiang, Xuanmin Guang, Le Cao, Jiawei Shi, Weikun Li, Jianglin Wang, Weiming He, Liyu Huang, Feng Yang, Yi-Xuan Bai, Sunil Kumar Sahu, Xing Guo, Shilai Zhang, Gengyun Zhang, Xun Xu, Fengyi Hu, Wanneng Yang, Huan Liu, Yu Zhao, Jun Lyu

{"title":"Comparative spatial transcriptomics reveals root dryland adaptation mechanism in rice and HMGB1 as a key regulator.","authors":"Liyuan Zhong, Leping Geng, Yimeng Xiang, Xuanmin Guang, Le Cao, Jiawei Shi, Weikun Li, Jianglin Wang, Weiming He, Liyu Huang, Feng Yang, Yi-Xuan Bai, Sunil Kumar Sahu, Xing Guo, Shilai Zhang, Gengyun Zhang, Xun Xu, Fengyi Hu, Wanneng Yang, Huan Liu, Yu Zhao, Jun Lyu","doi":"10.1016/j.molp.2025.04.001","DOIUrl":"10.1016/j.molp.2025.04.001","url":null,"abstract":"Drought severely threatens food security, and its detrimental effects will be exacerbated by climate change in many parts of the world. Rice production is water-consuming and particularly vulnerable to drought stress. Upland rice is a special rice ecotype that specifically adapts to dryland mainly due to its robust root system. However, the molecular and developmental mechanism underlying this adaption has remained elusive. In this study, by comparing the root development between upland and irrigated rice phenotypically and cytologically, we identified key developmental phenotypes that distinguish upland rice from irrigated rice. We further generated spatial transcriptomic atlases for coleoptilar nodes and root tips to explore their molecular differences in crown root formation and development, uncovering promising genes for enhancing rice drought resistance. Among the identified genes, HMGB1, a transcriptional regulator, functions as a key factor that facilitates root elongation and thickening in upland rice and thereby enhances drought resistance. In summary, our study uncovers spatially resolved transcriptomic features in roots of upland rice that contribute to its adaptation to dryland conditions, providing valuable genetic resources for breeding drought-resilient rice.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"797-819"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic transcriptome and GWAS uncover a hydroxyproline-rich glycoprotein that suppresses Agrobacterium-mediated transformation in maize. 动态转录组和GWAS揭示了富含羟脯氨酸的糖蛋白抑制农杆菌介导的玉米转化。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-19 DOI: 10.1016/j.molp.2025.03.011

Min Liu, Yan Yang, Tianhu Liang, Fengxia Hou, Minyan Zhang, Shijiang He, Peng Liu, Chaoying Zou, Langlang Ma, Guangtang Pan, Yaou Shen

{"title":"Dynamic transcriptome and GWAS uncover a hydroxyproline-rich glycoprotein that suppresses Agrobacterium-mediated transformation in maize.","authors":"Min Liu, Yan Yang, Tianhu Liang, Fengxia Hou, Minyan Zhang, Shijiang He, Peng Liu, Chaoying Zou, Langlang Ma, Guangtang Pan, Yaou Shen","doi":"10.1016/j.molp.2025.03.011","DOIUrl":"10.1016/j.molp.2025.03.011","url":null,"abstract":"Genetic transformation is a crucial tool for investigating gene function and advancing molecular breeding in crops, with Agrobacterium tumefaciens-mediated transformation being the primary method for plant genetic modification. However, this approach exhibits significant genotypic dependence in maize. Therefore, to overcome these limitations, we combined dynamic transcriptome analysis and genome-wide association study (GWAS) to identify the key genes controlling Agrobacterium infection frequency (AIF) in immature maize embryos. Transcriptome analysis of Agrobacterium-infected embryos uncovered 8483 and 1580 genotype-specific response genes in the maize line 18-599R with low AIF and A188 with high AIF, respectively. A weighted gene co-expression network analysis (WGCNA) revealed five and seven stage-specific co-expression modules in each corresponding line. Based on a self-developed AIF quantitation method, the GWAS revealed 30 AIF-associated single-nucleotide polymorphisms and 315 candidate genes under multiple environments. Integration of GWAS and WGCNA further identified 12 key genes associated with high AIF in A188. ZmHRGP, encoding a hydroxyproline-rich glycoprotein, was functionally validated as a key factor of AIF in immature embryos. Knockout of ZmHRGP enabled us to establish a high-efficiency genetic transformation system for the 18-599R line, with the transformation frequency being approximately 80%. Moreover, the transient reduction of ZmHRGP expression significantly enhanced the AIF of maize calluses and leaves. Collectively, these findings advance our understanding of plant factors controlling Agrobacterium infection and contribute to developing more efficient Agrobacterium-mediated transformation systems in crops.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"747-764"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct regulation of mRNA decay pathways by ABA enhances Nitrate Reductase 1/2-derived siRNAs production and stress adaptation. ABA对mRNA衰变途径的不同调控促进了硝酸还原酶1/2衍生sirna的产生和胁迫适应。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-04-18 DOI: 10.1016/j.molp.2025.04.007

Yan Yan, Yinpeng Xie, Qian Gao, Yajie Pan, Xianli Tang, Yuelin Liu, Wenyang Li, Hongwei Guo

{"title":"Distinct regulation of mRNA decay pathways by ABA enhances Nitrate Reductase 1/2-derived siRNAs production and stress adaptation.","authors":"Yan Yan, Yinpeng Xie, Qian Gao, Yajie Pan, Xianli Tang, Yuelin Liu, Wenyang Li, Hongwei Guo","doi":"10.1016/j.molp.2025.04.007","DOIUrl":"https://doi.org/10.1016/j.molp.2025.04.007","url":null,"abstract":"RNA degradation systems (e.g., RNA decay and RNA interference) and the phytohormone abscisic acid (ABA) are both essential for plant growth, development, and adaptation to stress. Although the interplay between these pathways has been recognized, the molecular mechanisms governing their coordination remain poorly understood. In this study, we revealed that mutations in the 5'-3' RNA-degrading enzyme Ethylene Insensitive 5 (EIN5) result in hypersensitivity to ABA in Arabidopsis, whereas defects in the 3'-5' RNA turnover machinery (ski mutants) do not. The ABA hypersensitivity of ein5 mutants was mitigated by mutating components of the post-transcriptional gene silencing (PTGS) pathway, including DICER-LIKE 2 (DCL2)/DCL4, RNA-Dependent RNA Polymerase 1 (RDR1)/RDR6, and ARGONAUTE 1 (AGO1). ABA treatment substantially increased the abundance of coding-transcript-derived small interfering RNAs (ct-siRNAs) in ein5, predominantly from two genes, Nitrate Reductase 1 (NIA1) and NIA2. Further analysis suggested that NIA1 and NIA2 negatively regulate both the ABA biosynthesis and signaling pathways. The key transcription factor Abscisic Acid Insensitive 3 (ABI3) represses SKI3 expression by directly binding to its promoter, thereby promoting the production of NIA1/NIA2-derived ct-siRNAs, leading to the ABA hypersensitivity of ein5. Conversely, ABA enhances the accumulation of EIN5 as well as DCL4 and AGO1, pointing to distinct regulation of the mRNA decay and PTGS pathways. Collectively, these findings demonstrate the pivotal roles of NIA1 and NIA2 in plant responses to abiotic stress and provide new insights into the interplay between the ABA response and RNA degradation pathways.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"18 5","pages":"853-871"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RAPID LEAF FALLING 1 facilitates chemical defoliation and mechanical harvesting in cotton. 快速落叶有利于棉花的化学落叶和机械收获。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-28 DOI: 10.1016/j.molp.2025.03.017

Bing Zhang, Dandan Yue, Bei Han, Danfan Bao, Xiao Zhang, Xuyang Hao, Xin Lin, Keith Lindsey, Longfu Zhu, Shuangxia Jin, Maojun Wang, Haijiang Xu, Mingwei Du, Yu Yu, Xianlong Zhang, Xiyan Yang

{"title":"RAPID LEAF FALLING 1 facilitates chemical defoliation and mechanical harvesting in cotton.","authors":"Bing Zhang, Dandan Yue, Bei Han, Danfan Bao, Xiao Zhang, Xuyang Hao, Xin Lin, Keith Lindsey, Longfu Zhu, Shuangxia Jin, Maojun Wang, Haijiang Xu, Mingwei Du, Yu Yu, Xianlong Zhang, Xiyan Yang","doi":"10.1016/j.molp.2025.03.017","DOIUrl":"10.1016/j.molp.2025.03.017","url":null,"abstract":"Chemical defoliation stands as the ultimate tool in enabling the mechanical harvest of cotton, offering economic and environmental advantages. However, the underlying molecular mechanism that triggers leaf abscission through defoliant remains unsolved. In this study, we meticulously constructed a transcriptomic atlas through single-nucleus mRNA sequencing (snRNA-seq) of the abscission zone (AZ) from cotton petiole. We identified two newly-formed cell types, abscission cells and protection layer cells in cotton petiole AZ after defoliant treatment. GhRLF1 (RAPID LEAF FALLING 1), as one of the members of the cytokinin oxidase/dehydrogenase (CKX) gene family, was further characterized as a key marker gene unique to the abscission cells following defoliant treatment. Overexpression of GhRLF1 resulted in reduced cytokinin accumulation and accelerated leaf abscission. Conversely, CRISPR/Cas9-mediated loss of GhRLF1 function appeared to delay this process. Its interacting regulators, GhWRKY70, acting as \"Pioneer\" activator, and GhMYB108, acting as \"Successor\" activator, orchestrate a sequential modulation of GhWRKY70/GhMYB108-GhRLF1-CTK (cytokinin) within the AZ to regulate cotton leaf abscission. GhRLF1 not only regulates leaf abscission but also reduces cotton yield. Consequently, transgenic lines that exhibit rapid leaf falling and require less defoliant but show unaffected cotton yield were developed for mechanical harvesting. This was achieved using a defoliant-induced petiole-specific promoter, proPER21, to drive GhRLF1 (proPER21::RLF1). This pioneering biotechnology offers a new strategy for the chemical defoliation of machine-harvested cotton, ensuring stable production and reducing leaf debris in harvested cotton, thereby enhancing environmental sustainability.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"765-782"},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogrammable design of DELLA as a strategy to mitigate alkaline-heat stress for sustainable agriculture. DELLA的可重新编程设计作为缓解可持续农业碱热胁迫的策略。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-03-28 DOI: 10.1016/j.molp.2025.03.015

Jie Hu, Hui Liu, Xiuhua Gao, Xiangdong Fu

引用次数: 0