Molecular Plant最新文献_第5页

SCOOP10 and SCOOP12 peptides act through MIK2 receptor-like kinase to antagonistically regulate Arabidopsis leaf senescence. SCOOP10 和 SCOOP12 肽通过 MIK2 受体样激酶拮抗拟南芥叶片衰老。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-28 DOI: 10.1016/j.molp.2024.10.010

Zhenbiao Zhang, Nora Gigli-Bisceglia, Wei Li, Saijie Li, Jie Wang, Junfeng Liu, Christa Testerink, Yongfeng Guo

{"title":"SCOOP10 and SCOOP12 peptides act through MIK2 receptor-like kinase to antagonistically regulate Arabidopsis leaf senescence.","authors":"Zhenbiao Zhang, Nora Gigli-Bisceglia, Wei Li, Saijie Li, Jie Wang, Junfeng Liu, Christa Testerink, Yongfeng Guo","doi":"10.1016/j.molp.2024.10.010","DOIUrl":"10.1016/j.molp.2024.10.010","url":null,"abstract":"Leaf senescence plays a critical role in a plant's overall reproductive success due to its involvement in nutrient remobilization and allocation. However, our current understanding of the molecular mechanisms controlling leaf senescence remains limited. In this study, we show that the receptor-like kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) functions as a negative regulator of leaf senescence. We found that the SERINE-RICH ENDOGENOUS PEPTIDE 12, previously known to physically interact with MIK2, competes with SCOOP10 to regulate MIK2-dependent leaf senescence. We observed that increased expression of SCOOP10 or the application of exogenous SCOOP10 peptides accelerated leaf senescence in a MIK2-dependent manner. Conversely, SCOOP12 acted as a suppressor of MIK2-dependent leaf senescence regulation. Biochemical assays showed that SCOOP12 enhances while SCOOP10 diminishes MIK2 phosphorylation. Thus, the SCOOP12-MIK2 module might function antagonistically on SCOOP10-MIK2 signaling at late senescing stages, allowing for fine-tuned modulation of the leaf senescence process. Our study sheds light on the complex mechanisms underlying leaf senescence and provides valuable insights into the interplay between receptors, peptides, and the regulation of plant senescence.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1805-1819"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The BCM1-EGY1 module balances chlorophyll biosynthesis and breakdown to confer chlorophyll homeostasis in land plants. BCM1-EGY1模块平衡叶绿素的生物合成和分解，赋予陆地植物叶绿素稳态。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 DOI: 10.1016/j.molp.2024.11.016

Dali Fu, Hanlin Zhou, Bernhard Grimm, Peng Wang

{"title":"The BCM1-EGY1 module balances chlorophyll biosynthesis and breakdown to confer chlorophyll homeostasis in land plants.","authors":"Dali Fu, Hanlin Zhou, Bernhard Grimm, Peng Wang","doi":"10.1016/j.molp.2024.11.016","DOIUrl":"10.1016/j.molp.2024.11.016","url":null,"abstract":"Chlorophyll metabolism has evolved during plant evolution. The strictly light-dependent nature of chlorophyll biosynthesis found in angiosperms requires tight coordination of chlorophyll biosynthesis and breakdown to achieve chlorophyll homeostasis. However, the specific control mechanisms remain largely unclear. Here, we demonstrate that the scaffold protein BALANCE OF CHLOROPHYLL METABOLISM1 (BCM1) has co-evolved with the carboxy-terminal domains of specific enzymes involved in chlorophyll biosynthesis and breakdown, including GENOMES UNCOUPLED 4 (GUN4) and Mg-dechelatase 1 (SGR1). We found that the land plant-specific interaction of BCM1 with the carboxy-terminal domains of GUN4 and SGR1 is indispensable for concurrent stimulation of chlorophyll biosynthesis and suppression of chlorophyll breakdown. The land plant-specific carboxy-terminal domain is essential for the membrane docking and turnover of GUN4, whereas it is key for proteolysis of SGR1. More importantly, we identified the metallopeptidase Gravitropism-deficient and Yellow-green 1 (EGY1) as the proteolytic machinery responsible for BCM1-mediated proteolysis of SGR1. In summary, this study reveals the BCM1-EGY1 module has evolved to maintain chlorophyll homeostasis by the post-translational control of the balance between chlorophyll biosynthesis and breakdown. This mechanism thus represents an evolutionary response to the metabolic demands imposed on plants in terrestrial environments.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unleashing strategies to mitigate methane emissions in rice fields and livestock systems. 释放缓解稻田和畜牧系统甲烷排放的战略。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-19 DOI: 10.1016/j.molp.2024.11.008

Ezhilmathi Angela Joseph Fernando, Michael Selvaraj, Jacobo Arango

引用次数: 0

The long non-coding RNA CARMA directs sucrose-responsive osmoregulation. 长非编码 RNA CARMA 引导蔗糖反应性渗透调节。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-13 DOI: 10.1016/j.molp.2024.11.005

Seungchul Lee, Ildoo Hwang

引用次数: 0

Heat stress-induced decapping of WUSCHEL mRNA enhances stem cell thermotolerance in Arabidopsis. 热胁迫诱导的 WUSCHEL mRNA 脱帽可增强拟南芥干细胞的耐热性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-28 DOI: 10.1016/j.molp.2024.10.011

Sumei Liu, Haijun Wu, Zhong Zhao

引用次数: 0

Volatilome-based GWAS identifies OsWRKY19 and OsNAC021 as key regulators of rice aroma. 基于挥发物的 GWAS 发现 OsWRKY19 和 OsNAC021 是水稻香气的关键调控因子。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-12 DOI: 10.1016/j.molp.2024.11.002

Yan Li, Yuanyuan Miao, Honglun Yuan, Fengkun Huang, Mingqi Sun, Liqiang He, Xianqing Liu, Jie Luo

{"title":"Volatilome-based GWAS identifies OsWRKY19 and OsNAC021 as key regulators of rice aroma.","authors":"Yan Li, Yuanyuan Miao, Honglun Yuan, Fengkun Huang, Mingqi Sun, Liqiang He, Xianqing Liu, Jie Luo","doi":"10.1016/j.molp.2024.11.002","DOIUrl":"10.1016/j.molp.2024.11.002","url":null,"abstract":"Aromatic rice is globally favored for its distinctive scent, which not only increases its nutritional value but also enhances its economic importance. However, apart from 2-acetyl-1-pyrroline (2-AP), the metabolic basis of aroma remains to be clarified, and the genetic basis of the accumulation of fragrance metabolites is largely unknown. In this study, we revealed 2-AP and fatty acid-derived volatiles (FAVs) as key contributors to rice aroma by combining aroma rating with molecular docking. Using a volatilome-based genome-wide association study, we identified two regulatory genes that determine the natural variation of these fragrance metabolites. Genetic and molecular analyses showed that OsWRKY19 not only enhances fragrance by negatively regulating OsBADH2 but also improves agricultural traits in rice. Furthermore, we revealed that OsNAC021 negatively regulates FAV contents via the lipoxygenase pathway, and its knockout resulted in over-accumulation of grain FAVs without a yield penalty. Collectively, our study not only identifies two key regulators of rice aroma but also provides a compelling example about how to deciphering the genetic regulatory mechanisms that underlie rice fragrance, thereby paving the way for the creation of aromatic rice varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1866-1882"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice. TT1-SCE1 模块整合了泛素化和 SUMOylation，以调控水稻的耐热性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-16 DOI: 10.1016/j.molp.2024.11.007

Hong-Xiao Yu, Ying-Jie Cao, Yi-Bing Yang, Jun-Xiang Shan, Wang-Wei Ye, Nai-Qian Dong, Yi Kan, Huai-Yu Zhao, Zi-Qi Lu, Shuang-Qin Guo, Jie-Jie Lei, Ben Liao, Hong-Xuan Lin

{"title":"A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice.","authors":"Hong-Xiao Yu, Ying-Jie Cao, Yi-Bing Yang, Jun-Xiang Shan, Wang-Wei Ye, Nai-Qian Dong, Yi Kan, Huai-Yu Zhao, Zi-Qi Lu, Shuang-Qin Guo, Jie-Jie Lei, Ben Liao, Hong-Xuan Lin","doi":"10.1016/j.molp.2024.11.007","DOIUrl":"10.1016/j.molp.2024.11.007","url":null,"abstract":"Heat stress poses a significant threat to grain yield. As an α2 subunit of the 26S proteasome, TT1 has been shown to act as a critical regulator of rice heat tolerance. However, the heat tolerance mechanisms mediated by TT1 remain elusive. In this study, we unveiled that small ubiquitin-like modifier (SUMO)-conjugating enzyme 1 (SCE1), which interacts with TT1 and acts as a downstream component of TT1, is engaged in TT1-mediated 26S proteasome degradation. We showed that SCE1 functions as a negative regulator of heat tolerance in rice, which is associated with its ubiquitination modification. Furthermore, we observed that small heat-shock proteins (sHSPs) such as Hsp24.1 and Hsp40 can undergo SUMOylation mediated by SCE1, leading to increased accumulation of sHSPs in the absence of SCE1. Reducing protein levels of SCE1 significantly enhanced grain yield under high-temperature stress by improving seed-setting rate and rice grain filling capacity. Taken together, these results uncover the critical role of SCE1 in the TT1-mediated heat tolerance pathway by regulating the abundance of sHSPs and SUMOylation, and ultimately modulating rice heat tolerance. These findings underscore the great potential of the TT1-SCE1 module in improving the heat tolerance of crops.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1899-1918"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A metabolic roadmap of waxy corn flavor. 蜡质玉米风味的代谢路线图

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-12 DOI: 10.1016/j.molp.2024.11.003

Jingyun Luo, Chunmei He, Shijuan Yan, Chenglin Jiang, An Chen, Kun Li, Yongli Zhu, Songtao Gui, Ning Yang, Yingjie Xiao, Shenshen Wu, Fajun Zhang, Tieshan Liu, Juan Wang, Wenjie Huang, Yanhua Yang, Haiyan Wang, Wenyu Yang, Wenqiang Li, Lin Zhuo, Alisdair R Fernie, Junpeng Zhan, Liming Wang, Jianbing Yan

{"title":"A metabolic roadmap of waxy corn flavor.","authors":"Jingyun Luo, Chunmei He, Shijuan Yan, Chenglin Jiang, An Chen, Kun Li, Yongli Zhu, Songtao Gui, Ning Yang, Yingjie Xiao, Shenshen Wu, Fajun Zhang, Tieshan Liu, Juan Wang, Wenjie Huang, Yanhua Yang, Haiyan Wang, Wenyu Yang, Wenqiang Li, Lin Zhuo, Alisdair R Fernie, Junpeng Zhan, Liming Wang, Jianbing Yan","doi":"10.1016/j.molp.2024.11.003","DOIUrl":"10.1016/j.molp.2024.11.003","url":null,"abstract":"As well as being a popular vegetable crop worldwide, waxy corn represents an important amylopectin source, but little is known about its breeding history and flavor characteristics. In this study, through comparative-omic analyses between 318 diverse waxy corn and 507 representative field corn inbred lines we revealed that many metabolic pathways and genes exhibited selection characteristics during the breeding history of waxy corn, contributing to the divergence between waxy and field corn. We showed that waxy corn is not only altered in its glutinous property but also its sweetness, aroma, and palatability are all significantly affected. A substantial proportion (43%) of flavor-related metabolites have pleiotropic effects, affecting both flavor and yield characteristics, and 27% of these metabolites are related to antagonistic outcomes on yield and flavor. Furthermore, through multiple concrete examples, we demonstrated how yield and quality are coordinately or antagonistically regulated at the genetic level. In particular, some sweet molecules, such as DIMBOA and raffinose, which do not participate in the starch biosynthesis pathway, were identified as potential targets for breeding a new type of \"sweet-waxy\" corn. Taken together, our findings shed light on the historical selection of waxy corn and demonstrate the genetic and metabolic basis of waxy corn flavor, collectively providing valuable resources and knowledge for future crop breeding for improved nutritional quality.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1883-1898"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribosome-associated quality control underlies rice thermo-sensitive genic male sterility. 核糖体相关质量控制是水稻热敏性雄性不育的基础。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-30 DOI: 10.1016/j.molp.2024.11.014

Takahiko Kubo

引用次数: 0

Allelic variation in the promoter of WRKY22 enhances humid adaptation of Arabidopsisthaliana. WRKY22启动子等位基因变异增强拟南芥湿润适应性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-29 DOI: 10.1016/j.molp.2024.11.013

Ruyun Liang, Luna Tan, Xiang Guo, Shangling Lou, Xuming Dan, Yu Han, Cheng Zeng, Han Zhang, Kai Yang, Liyang Chen, Xin Liang, Meng Liu, Mengyun Guo, Kangqun Yin, Si Tang, Yan Song, Xuemeng Gao, Shaobo Gu, Jing Hou, Yingjun Yao, Ruijia Zhang, Jin Yan, Wensen Fu, Xuerui Li, Yongqi Hu, Yao Liu, Wei Liu, Qiusai Wu, Zhen Yan, Weitao Jia, Binhua Hu, Jing Wang, Jianquan Liu, Huanhuan Liu

{"title":"Allelic variation in the promoter of WRKY22 enhances humid adaptation of Arabidopsisthaliana.","authors":"Ruyun Liang, Luna Tan, Xiang Guo, Shangling Lou, Xuming Dan, Yu Han, Cheng Zeng, Han Zhang, Kai Yang, Liyang Chen, Xin Liang, Meng Liu, Mengyun Guo, Kangqun Yin, Si Tang, Yan Song, Xuemeng Gao, Shaobo Gu, Jing Hou, Yingjun Yao, Ruijia Zhang, Jin Yan, Wensen Fu, Xuerui Li, Yongqi Hu, Yao Liu, Wei Liu, Qiusai Wu, Zhen Yan, Weitao Jia, Binhua Hu, Jing Wang, Jianquan Liu, Huanhuan Liu","doi":"10.1016/j.molp.2024.11.013","DOIUrl":"10.1016/j.molp.2024.11.013","url":null,"abstract":"Submergence stress tolerance is a complex trait governed by multiple loci. Because of its wide distribution across arid and humid regions, Arabidopsis thaliana offers an opportunity to explore the genetic components and their action mechanisms underlying plant adaptation to submergence stress. In this study, using map-based cloning we identified WRKY22 that activates RAP2.12, a locus previously identified to contribute to the submergence stress response, to regulate plant humid adaptation possibly through ethylene signal transduction in Arabidopsis. WRKY22 expression is inhibited by ARABIDOPSIS RESPONSE REGULATORs (ARRs) but activated by the WRKY70 transcription factor. In accessions from humid environments, a two-nucleotide deletion in the WRKY22 promoter region prevents binding of phosphorylated ARRs, thereby maintaining its high expression. Loss of the ARR-binding element in the WRKY22 promoter underwent strong positive selection during colonization of A. thaliana in the humid Yangtze River basin. However, the WRKY70-binding motif in the WRKY22 promoter shows no variation between accessions from humid and arid regions. These findings together establish a novel signaling axis wherein WRKY22 plays a key role in regulating the adaptive response that enables A. thaliana to colonize contrasting habitats. Notably, we further showed functional conservation of this locus in Brassica napus, suggesting that modulating this axis might be useful in the breeding of flood-tolerant crop varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":""},"PeriodicalIF":17.1,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0