Molecular Plant最新文献_第10页

SIZ1-mediated SUMOylation of CPSF100 promotes plant thermomorphogenesis by controlling alternative polyadenylation. SIZ1介导的CPSF100的SUMOlation通过控制替代多聚腺苷酸化促进植物的热形态发生。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-25 DOI: 10.1016/j.molp.2024.07.011

Zhibo Yu, Jun Wang, Cheng Zhang, Qiuna Zhan, Leqian Shi, Bing Song, Danlu Han, Jieming Jiang, Junwen Huang, Xiaolin Ou, Zhonghui Zhang, Jianbin Lai, Qingshun Quinn Li, Chengwei Yang

{"title":"SIZ1-mediated SUMOylation of CPSF100 promotes plant thermomorphogenesis by controlling alternative polyadenylation.","authors":"Zhibo Yu, Jun Wang, Cheng Zhang, Qiuna Zhan, Leqian Shi, Bing Song, Danlu Han, Jieming Jiang, Junwen Huang, Xiaolin Ou, Zhonghui Zhang, Jianbin Lai, Qingshun Quinn Li, Chengwei Yang","doi":"10.1016/j.molp.2024.07.011","DOIUrl":"10.1016/j.molp.2024.07.011","url":null,"abstract":"Under warm temperatures, plants adjust their morphologies for environmental adaption via precise gene expression regulation. However, the function and regulation of alternative polyadenylation (APA), an important fine-tuning of gene expression, remains unknown in plant thermomorphogenesis. In this study, we found that SUMOylation, a critical post-translational modification, is induced by a long-term treatment at warm temperatures via a SUMO ligase SIZ1 in Arabidopsis. Disruption of SIZ1 altered the global usage of polyadenylation signals and affected the APA dynamic of thermomorphogenesis-related genes. CPSF100, a key subunit of the CPSF complex for polyadenylation regulation, is SUMOylated by SIZ1. Importantly, we demonstrated that SUMOylation is essential for the function of CPSF100 in genome-wide polyadenylation site choice during thermomorphogenesis. Further analyses revealed that the SUMO conjugation on CPSF100 attenuates its interaction with two isoforms of its partner CPSF30, increasing the nuclear accumulation of CPSF100 for polyadenylation regulation. In summary, our study uncovers a regulatory mechanism of APA via SIZ1-mediated SUMOylation in plant thermomorphogenesis.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1392-1406"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple. ACC 氧化酶 3 mRNA 从种子到果肉的移动促进了苹果果实的成熟。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-19 DOI: 10.1016/j.molp.2024.06.008

Ting Wang, Yi Zheng, Chen Xu, Yulin Deng, Xinyi Hao, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu

{"title":"Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple.","authors":"Ting Wang, Yi Zheng, Chen Xu, Yulin Deng, Xinyi Hao, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu","doi":"10.1016/j.molp.2024.06.008","DOIUrl":"10.1016/j.molp.2024.06.008","url":null,"abstract":"Xenia, the phenomenon in which the pollen genotype directly affects the phenotypic characteristics of maternal tissues (i.e., fruit ripening), has applications in crop production and breeding. However, the underlying molecular mechanism has yet to be elucidated. Here, we investigated whether mobile mRNAs from the pollen affect the ripening and quality-related characteristics of the fruit using cross-pollination between distinct Malus domestica (apple) cultivars. We demonstrated that hundreds of mobile mRNAs originating from the seeds are delivered to the fruit. We found that the movement of one of these mRNAs, ACC oxidase 3 (MdACO3), is coordinated with fruit ripening. Salicylic acid treatment, which can cause plasmodesmal closure, blocks MdACO3 movement, indicating that MdACO3 transcripts may move through the plasmodesmata. To assess the role of mobile MdACO3 transcripts in apple fruit, we created MdACO3-GFP-expressing apple seeds using MdACO3-GFP-overexpressing pollen for pollination and showed that MdACO3 transcripts in the transgenic seeds move to the flesh, where they promote fruit ripening. Furthermore, we demonstrated that MdACO3 can be transported from the seeds to fruit in the fleshy-fruited species tomato and strawberry. These results underscore the potential of mobile mRNAs from seeds to influence fruit characteristics, providing an explanation for the xenia phenomenon. Notably, our findings highlight the feasibility of leveraging diverse pollen genomic resources, without resorting to genome editing, to improve fruit quality.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1221-1235"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141432352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging plant biomechanics in multiscale plant systems for sustainable innovations. 在多尺度植物系统中利用植物生物力学进行可持续创新。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-05 DOI: 10.1016/j.molp.2024.07.002

Jinbo Shen, Yansong Miao

引用次数: 0

Development and maintenance of the ligular region of maize leaves. 玉米叶片韧带区的发育和维持。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-11 DOI: 10.1016/j.molp.2024.07.004

Josh Strable, Alejandro Aragón-Raygoza

引用次数: 0

CONSTANS alters the circadian clock in Arabidopsis thaliana. CONSTANS 改变了拟南芥的昼夜节律。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-17 DOI: 10.1016/j.molp.2024.06.006

Pedro de Los Reyes, Gloria Serrano-Bueno, Francisco J Romero-Campero, He Gao, Jose M Romero, Federico Valverde

{"title":"CONSTANS alters the circadian clock in Arabidopsis thaliana.","authors":"Pedro de Los Reyes, Gloria Serrano-Bueno, Francisco J Romero-Campero, He Gao, Jose M Romero, Federico Valverde","doi":"10.1016/j.molp.2024.06.006","DOIUrl":"10.1016/j.molp.2024.06.006","url":null,"abstract":"Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment. Among these processes, the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks. The photoperiodic pathway, which controls plant response to day length, is one of the most important pathways controlling flowering. In Arabidopsis photoperiodic flowering, CONSTANS (CO) is the central gene activating the expression of the florigen FLOWERING LOCUS T (FT) in the leaves at the end of a long day. The circadian clock strongly regulates CO expression. However, to date, no evidence has been reported regarding a feedback loop from the photoperiod pathway back to the circadian clock. Using transcriptional networks, we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod pathway. Gene expression, chromatin immunoprecipitation experiments, and phenotypic analysis allowed us to elucidate the role of CO over the circadian clock. Plants with altered CO expression showed a different internal clock period, measured by daily leaf rhythmic movements. We showed that CO upregulates the expression of key genes related to the circadian clock, such as CCA1, LHY, PRR5, and GI, at the end of a long day by binding to specific sites on their promoters. Moreover, a high number of PRR5-repressed target genes are upregulated by CO, and this could explain the phase transition promoted by CO. The CO-PRR5 complex interacts with the bZIP transcription factor HY5 and helps to localize the complex in the promoters of clock genes. Taken together, our results indicate that there may be a feedback loop in which CO communicates back to the circadian clock, providing seasonal information to the circadian system.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1204-1220"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixing and matching SMXL proteins to fine-tune strigolactone responses. 混合和匹配 SMXL 蛋白以微调绞股蓝内酯反应。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-18 DOI: 10.1016/j.molp.2024.06.009

Jenna E Hountalas, Shelley Lumba

引用次数: 0

A near-complete cucumber reference genome assembly and Cucumber-DB, a multi-omics database. 近乎完整的黄瓜参考基因组组装和多组学数据库 Cucumber-DB。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-20 DOI: 10.1016/j.molp.2024.06.012

Jiantao Guan, Han Miao, Zhonghua Zhang, Shaoyun Dong, Qi Zhou, Xiaoping Liu, Diane M Beckles, Xingfang Gu, Sanwen Huang, Shengping Zhang

引用次数: 0

Phenotyping floral attractiveness to pollinators using volatilomics, 3D imaging, and insect monitoring. 利用挥发物组学、三维成像和昆虫监测对花朵对传粉昆虫的吸引力进行表型分析。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-20 DOI: 10.1016/j.molp.2024.06.011

Filip Slavković, Adnane Boualem, Catherine Dogimont, Abdelhafid Bendahmane

引用次数: 0

Steroidal scaffold decorations in Solanum alkaloid biosynthesis. 茄科生物碱生物合成中的类固醇支架装饰。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-26 DOI: 10.1016/j.molp.2024.06.013

Rosalind Lucier, Mohamed O Kamileen, Yoko Nakamura, Sofiia Serediuk, Ranjit Barbole, Jens Wurlitzer, Maritta Kunert, Sarah Heinicke, Sarah E O'Connor, Prashant D Sonawane

{"title":"Steroidal scaffold decorations in Solanum alkaloid biosynthesis.","authors":"Rosalind Lucier, Mohamed O Kamileen, Yoko Nakamura, Sofiia Serediuk, Ranjit Barbole, Jens Wurlitzer, Maritta Kunert, Sarah Heinicke, Sarah E O'Connor, Prashant D Sonawane","doi":"10.1016/j.molp.2024.06.013","DOIUrl":"10.1016/j.molp.2024.06.013","url":null,"abstract":"Steroidal glycoalkaloids (SGAs) are specialized metabolites produced by hundreds of Solanum species, including important vegetable crops such as tomato, potato, and eggplant. Although it has been known that SGAs play important roles in defense in plants and \"anti-nutritional\" effects (e.g., toxicity and bitterness) to humans, many of these molecules have documented anti-cancer, anti-microbial, anti-inflammatory, anti-viral, and anti-pyretic activities. Among these, α-solasonine and α-solamargine isolated from black nightshade (Solanum nigrum) are reported to have potent anti-tumor, anti-proliferative, and anti-inflammatory activities. Notably, α-solasonine and α-solamargine, along with the core steroidal aglycone solasodine, are the most widespread SGAs produced among the Solanum plants. However, it is still unknown how plants synthesize these bioactive steroidal molecules. Through comparative metabolomic-transcriptome-guided approach, biosynthetic logic, combinatorial expression in Nicotiana benthamiana, and functional recombinant enzyme assays, here we report the discovery of 12 enzymes from S. nigrum that converts the starting cholesterol precursor to solasodine aglycone, and the downstream α-solasonine, α-solamargine, and malonyl-solamargine SGA products. We further identified six enzymes from cultivated eggplant that catalyze the production of α-solasonine, α-solamargine, and malonyl-solamargine SGAs from solasodine aglycone via glycosylation and atypical malonylation decorations. Our work provides the gene tool box and platform for engineering the production of high-value, steroidal bioactive molecules in heterologous hosts using synthetic biology.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1236-1254"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochrome B interacts with LIGULELESS1 to control plant architecture and density tolerance in maize. 植物色素 B 与 LIGULELESS1 相互作用，控制玉米的植株结构和耐密度。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-29 DOI: 10.1016/j.molp.2024.06.014

Qingbiao Shi, Ying Xia, Qibin Wang, Kaiwen Lv, Hengjia Yang, Lianzhe Cui, Yue Sun, Xiaofei Wang, Qing Tao, Xiehai Song, Di Xu, Wenchang Xu, Xingyun Wang, Xianglan Wang, Fanying Kong, Haisen Zhang, Bosheng Li, Pinghua Li, Haiyang Wang, Gang Li

{"title":"Phytochrome B interacts with LIGULELESS1 to control plant architecture and density tolerance in maize.","authors":"Qingbiao Shi, Ying Xia, Qibin Wang, Kaiwen Lv, Hengjia Yang, Lianzhe Cui, Yue Sun, Xiaofei Wang, Qing Tao, Xiehai Song, Di Xu, Wenchang Xu, Xingyun Wang, Xianglan Wang, Fanying Kong, Haisen Zhang, Bosheng Li, Pinghua Li, Haiyang Wang, Gang Li","doi":"10.1016/j.molp.2024.06.014","DOIUrl":"10.1016/j.molp.2024.06.014","url":null,"abstract":"Over the past few decades, significant improvements in maize yield have been largely attributed to increased plant density of upright hybrid varieties rather than increased yield per plant. However, dense planting triggers shade avoidance responses (SARs) that optimize light absorption but impair plant vigor and performance, limiting yield improvement through increasing plant density. In this study, we demonstrated that high-density-induced leaf angle narrowing and stem/stalk elongation are largely dependent on phytochrome B (phyB1/B2), the primary photoreceptor responsible for perceiving red (R) and far-red (FR) light in maize. We found that maize phyB physically interacts with the LIGULELESS1 (LG1), a classical key regulator of leaf angle, to coordinately regulate plant architecture and density tolerance. The abundance of LG1 is significantly increased by phyB under high R:FR light (low density) but rapidly decreases under low R:FR light (high density), correlating with variations in leaf angle and plant height under various densities. In addition, we identified the homeobox transcription factor HB53 as a target co-repressed by both phyB and LG1 but rapidly induced by canopy shade. Genetic and cellular analyses showed that HB53 regulates plant architecture by controlling the elongation and division of ligular adaxial and abaxial cells. Taken together, these findings uncover the phyB-LG1-HB53 regulatory module as a key molecular mechanism governing plant architecture and density tolerance, providing potential genetic targets for breeding maize hybrid varieties suitable for high-density planting.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1255-1271"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0