Genomic, transcriptomic, and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin's orchid.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Kai Jiang, Birger Lindberg Møller, Shaofan Luo, Yu Yang, David R Nelson, Elizabeth Heather Jakobsen Neilson, Joachim Møller Christensen, Kai Hua, Chao Hu, Xinhua Zeng, Mohammed Saddik Motawie, Tao Wan, Guang-Wan Hu, Guy Eric Onjalalaina, Yijiao Wang, Juan Diego Gaitán-Espitia, Zhiwen Wang, Xiao-Yan Xu, Jiamin He, Linying Wang, Yuanyuan Li, Dong-Hui Peng, Siren Lan, Huiming Zhang, Qing-Feng Wang, Zhong-Jian Liu, Wei-Chang Huang
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引用次数: 0

Abstract

Angraecum sesquipedale, also known as Darwin's orchid, possesses an exceptionally long nectar spur. Charles Darwin predicted the orchid to be pollinated by a hawkmoth with a correspondingly long proboscis, later identified as Xanthopan praedicta. In this plant-pollinator interaction, the A. sesquipedale flower emits a complex blend of scent compounds dominated by diurnally regulated oximes (R1R2C=N-OH) to attract crepuscular and nocturnal pollinators. The molecular mechanism of oxime biosynthesis remains unclear in orchids. Here, we present the chromosome-level genome of A. sesquipedale. The haploid genome size is 2.10 Gb and represents 19 pseudochromosomes. Cytochrome P450 encoding genes of the CYP79 family known to be involved in oxime biosynthesis in seed plants are not present in the A. sesquipedale genome nor in the genomes of other members of the orchid family. Metabolomic analysis of the A. sesquipedale flower revealed a substantial release of oximes at dusk during the blooming stage. By integrating metabolomic and transcriptomic correlation approaches, flavin-containing monooxygenases (FMOs) encoded by six tandem-repeat genes in the A. sesquipedale genome are identified as catalyzing the formation of oximes present. Further in vitro and in vivo assays confirm the function of FMOs in the oxime biosynthesis. We designate these FMOs as Orchid Oxime Synthases 1-6. The evolutionary aspects related to the CYP79 gene losses and neofunctionalization of FMO-catalyzed biosynthesis of oximes in Darwin's orchid provide new insights into the convergent evolution of biosynthetic pathways.

基因组学、转录组学和代谢组学分析揭示了达尔文兰花的肟生物合成趋同进化。
Angraecum sesquipedale,也被称为达尔文的兰花,拥有特别长的花蜜刺。查尔斯·达尔文曾预言,兰花是由一种具有相应长喙的飞蛾授粉的,后来被鉴定为黄原花。在这种植物与传粉者的相互作用中,a . sesquipedale花释放出一种复杂的气味混合物,以昼夜调节的肟(R1R2C=N-OH)为主,以吸引黄昏和夜间传粉者。兰花中肟生物合成的分子机制尚不清楚。在这里,我们提出了A. sesquipedale的染色体水平基因组。单倍体基因组大小为2.10 Gb,有19条假染色体。已知参与种子植物肟生物合成的CYP79家族的细胞色素P450编码基因不存在于A. sesquipedale基因组中,也不存在于兰科其他成员的基因组中。代谢组学分析显示,在开花阶段的黄昏,大量释放肟。通过整合代谢组学和转录组学相关方法,鉴定了A. sesquipedale基因组中6个串联重复基因编码的含黄素单加氧酶(FMOs)催化了肟的形成。进一步的体外和体内实验证实了FMOs在肟生物合成中的作用。我们将这些FMOs命名为兰花肟合成酶1-6。达尔文兰中CYP79基因丢失和fmo催化的肟类生物合成的新功能化的进化方面为生物合成途径的趋同进化提供了新的见解。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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