Molecular Plant最新文献_第9页

Navigating biomolecular condensates in plants from patterns to functions. 从模式到功能的植物凝结物导航。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-14 DOI: 10.1016/j.molp.2024.08.001

Yansong Miao, Monika Chodasiewicz, Xiaofeng Fang

引用次数: 0

Genetic variation in a heat shock transcription factor modulates cold tolerance in maize. 热休克转录因子的遗传变异调节玉米的耐寒性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-07 DOI: 10.1016/j.molp.2024.07.015

Lei Gao, Lingling Pan, Yiting Shi, Rong Zeng, Minze Li, Zhuoyang Li, Xuan Zhang, Xiaoming Zhao, Xinru Gong, Wei Huang, Xiaohong Yang, Jinsheng Lai, Jianru Zuo, Zhizhong Gong, Xiqing Wang, Weiwei Jin, Zhaobin Dong, Shuhua Yang

{"title":"Genetic variation in a heat shock transcription factor modulates cold tolerance in maize.","authors":"Lei Gao, Lingling Pan, Yiting Shi, Rong Zeng, Minze Li, Zhuoyang Li, Xuan Zhang, Xiaoming Zhao, Xinru Gong, Wei Huang, Xiaohong Yang, Jinsheng Lai, Jianru Zuo, Zhizhong Gong, Xiqing Wang, Weiwei Jin, Zhaobin Dong, Shuhua Yang","doi":"10.1016/j.molp.2024.07.015","DOIUrl":"10.1016/j.molp.2024.07.015","url":null,"abstract":"Understanding how maize (Zea mays) responds to cold stress is crucial for facilitating breeding programs of cold-tolerant varieties. Despite extensive utilization of the genome-wide association study (GWAS) approach for exploring favorable natural alleles associated with maize cold tolerance, few studies have successfully identified candidate genes that contribute to maize cold tolerance. In this study, we used a diverse panel of inbred maize lines collected from different germplasm sources to perform a GWAS on variations in the relative injured area of maize true leaves during cold stress-a trait very closely correlated with maize cold tolerance. We identified HSF21, which encodes a B-class heat shock transcription factor (HSF) that positively regulates cold tolerance at both the seedling and germination stages. Natural variations in the promoter of the cold-tolerant HSF21Hap1 allele led to increased HSF21 expression under cold stress by inhibiting binding of the basic leucine zipper bZIP68 transcription factor, a negative regulator of cold tolerance. By integrating transcriptome deep sequencing, DNA affinity purification sequencing, and targeted lipidomic analysis, we revealed the function of HSF21 in regulating lipid metabolism homeostasis to modulate cold tolerance in maize. In addition, we found that HSF21 confers maize cold tolerance without incurring yield penalties. Collectively, this study establishes HSF21 as a key regulator that enhances cold tolerance in maize, providing valuable genetic resources for breeding of cold-tolerant maize varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1423-1438"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ubiquitination-mediated regulation of receptor-like kinases in symbiosis and immunity. 共生和免疫中类似受体激酶的泛素化调控

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-30 DOI: 10.1016/j.molp.2024.07.013

David Landry, Benoit Lefebvre

引用次数: 0

Candy or poison: Plant metabolites as swing factors against microbes. 糖果还是毒药植物代谢物是对抗微生物的摇摆因子

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-18 DOI: 10.1016/j.molp.2024.08.005

Liyuan Wang, Yu Xia, Yingnan Hou

引用次数: 0

REGENERATION FACTOR 1, a peptide boost for wound healing and plant biotechnology. 再生因子 1，一种促进伤口愈合和植物生物技术的多肽。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-26 DOI: 10.1016/j.molp.2024.07.012

Andreas Schaller

引用次数: 0

Arabidopsis photoperiodic regulator CONSTANS feeds back to control the circadian clock. 拟南芥中的光周期调节因子 CO 反馈控制昼夜节律。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-14 DOI: 10.1016/j.molp.2024.08.002

Abril San Martin, Marcelo Javier Yanovsky

引用次数: 0

Single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in St. John's wort. 单细胞 RNA 测序有助于阐明圣约翰草中抗抑郁剂金丝桃素的完整生物合成过程。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-12 DOI: 10.1016/j.molp.2024.08.003

Song Wu, Ana Luisa Malaco Morotti, Jun Yang, Ertao Wang, Evangelos C Tatsis

{"title":"Single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in St. John's wort.","authors":"Song Wu, Ana Luisa Malaco Morotti, Jun Yang, Ertao Wang, Evangelos C Tatsis","doi":"10.1016/j.molp.2024.08.003","DOIUrl":"10.1016/j.molp.2024.08.003","url":null,"abstract":"Hyperforin is the compound responsible for the effectiveness of St. John's wort (Hypericum perforatum) as an antidepressant, but its complete biosynthetic pathway remains unknown. Gene discovery based on co-expression analysis of bulk RNA-sequencing data or genome mining failed to discover the missing steps in hyperforin biosynthesis. In this study, we sequenced the 1.54-Gb tetraploid H. perforatum genome assembled into 32 chromosomes with the scaffold N50 value of 42.44 Mb. By single-cell RNA sequencing, we identified a type of cell, \"Hyper cells\", wherein hyperforin biosynthesis de novo takes place in both the leaves and flowers. Through pathway reconstitution in yeast and tobacco, we identified and characterized four transmembrane prenyltransferases (HpPT1-4) that are localized at the plastid envelope and complete the hyperforin biosynthetic pathway. The hyperforin polycyclic scaffold is created by a reaction cascade involving an irregular isoprenoid coupling and a tandem cyclization. Our findings reveal how and where hyperforin is biosynthesized, enabling synthetic-biology reconstitution of the complete pathway. Thus, this study not only deepens our comprehension of specialized metabolism at the cellular level but also provides strategic guidance for elucidation of the biosynthetic pathways of other specializied metabolites in plants.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1439-1457"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulation of photosensory and circadian signaling restricts phenotypic plasticity in response to changing environmental conditions in Arabidopsis. 操纵光感和昼夜节律信号可限制拟南芥对环境条件变化的表型可塑性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-15 DOI: 10.1016/j.molp.2024.07.007

Martin William Battle, Scott Fraser Ewing, Cathryn Dickson, Joseph Obaje, Kristen N Edgeworth, Rebecca Bindbeutel, Rea L Antoniou-Kourounioti, Dmitri A Nusinow, Matthew Alan Jones

{"title":"Manipulation of photosensory and circadian signaling restricts phenotypic plasticity in response to changing environmental conditions in Arabidopsis.","authors":"Martin William Battle, Scott Fraser Ewing, Cathryn Dickson, Joseph Obaje, Kristen N Edgeworth, Rebecca Bindbeutel, Rea L Antoniou-Kourounioti, Dmitri A Nusinow, Matthew Alan Jones","doi":"10.1016/j.molp.2024.07.007","DOIUrl":"10.1016/j.molp.2024.07.007","url":null,"abstract":"Plants exploit phenotypic plasticity to adapt their growth and development to prevailing environmental conditions. Interpretation of light and temperature signals is aided by the circadian system, which provides a temporal context. Phenotypic plasticity provides a selective and competitive advantage in nature but is obstructive during large-scale, intensive agricultural practices since economically important traits (including vegetative growth and flowering time) can vary widely depending on local environmental conditions. This prevents accurate prediction of harvesting times and produces a variable crop. In this study, we sought to restrict phenotypic plasticity and circadian regulation by manipulating signaling systems that govern plants' responses to environmental signals. Mathematical modeling of plant growth and development predicted reduced plant responses to changing environments when circadian and light signaling pathways were manipulated. We tested this prediction by utilizing a constitutively active allele of the plant photoreceptor phytochrome B, along with disruption of the circadian system via mutation of EARLY FLOWERING3. We found that these manipulations produced plants that are less responsive to light and temperature cues and thus fail to anticipate dawn. These engineered plants have uniform vegetative growth and flowering time, demonstrating how phenotypic plasticity can be limited while maintaining plant productivity. This has significant implications for future agriculture in both open fields and controlled environments.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1458-1471"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dose-dependent bimodal switch by homologous Aux/IAA transcriptional repressors. 同源 Aux/IAA 转录抑制因子的剂量依赖性双模切换。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI: 10.1016/j.molp.2024.07.014

Hyung-Taeg Cho, Minsu Lee, Hee-Seung Choi, Kwang-Ho Maeng, Kyeonghoon Lee, Ha-Yeon Lee, Anindya Ganguly, Hoonyoung Park, Chang-Hoi Ho

{"title":"A dose-dependent bimodal switch by homologous Aux/IAA transcriptional repressors.","authors":"Hyung-Taeg Cho, Minsu Lee, Hee-Seung Choi, Kwang-Ho Maeng, Kyeonghoon Lee, Ha-Yeon Lee, Anindya Ganguly, Hoonyoung Park, Chang-Hoi Ho","doi":"10.1016/j.molp.2024.07.014","DOIUrl":"10.1016/j.molp.2024.07.014","url":null,"abstract":"Combinatorial interactions between different regulators diversify and enrich the chance of transcriptional regulation in eukaryotic cells. However, a dose-dependent functional switch of homologous transcriptional repressors has rarely been reported. Here, we show that SHY2, an auxin/indole-3-acetic acid (Aux/IAA) repressor, exhibits a dose-dependent bimodal role in auxin-sensitive root-hair growth and gene transcription in Arabidopsis, whereas other Aux/IAA homologs consistently repress the auxin responses. The co-repressor (TOPLESS [TPL])-binding affinity of a bimodal Aux/IAA was lower than that of a consistently repressing Aux/IAA. The switch of a single amino acid residue in the TPL-binding motif between the bimodal form and the consistently repressing form switched their TPL-binding affinity and transcriptional and biological roles in auxin responses. Based on these data, we propose a model whereby competition between homologous repressors with different co-repressor-binding affinities could generate a bimodal output at the transcriptional and developmental levels.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1407-1422"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proxitome profiling reveals a conserved SGT1-NSL1 signaling module that activates NLR-mediated immunity. Proxitome 图谱揭示了激活 NLR 介导的免疫的保守 SGT1-NSL1 信号模块。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-25 DOI: 10.1016/j.molp.2024.07.010

Dingliang Zhang, Xinxin Yang, Zhiyan Wen, Zhen Li, Xinyu Zhang, Chenchen Zhong, Jiajie She, Qianshen Zhang, He Zhang, Wenli Li, Xiaoyun Zhao, Mingliang Xu, Zhen Su, Dawei Li, Savithramma P Dinesh-Kumar, Yongliang Zhang

{"title":"Proxitome profiling reveals a conserved SGT1-NSL1 signaling module that activates NLR-mediated immunity.","authors":"Dingliang Zhang, Xinxin Yang, Zhiyan Wen, Zhen Li, Xinyu Zhang, Chenchen Zhong, Jiajie She, Qianshen Zhang, He Zhang, Wenli Li, Xiaoyun Zhao, Mingliang Xu, Zhen Su, Dawei Li, Savithramma P Dinesh-Kumar, Yongliang Zhang","doi":"10.1016/j.molp.2024.07.010","DOIUrl":"10.1016/j.molp.2024.07.010","url":null,"abstract":"Suppressor of G2 allele of skp1 (SGT1) is a highly conserved eukaryotic protein that plays a vital role in growth, development, and immunity in both animals and plants. Although some SGT1 interactors have been identified, the molecular regulatory network of SGT1 remains unclear. SGT1 serves as a co-chaperone to stabilize protein complexes such as the nucleotide-binding leucine-rich repeat (NLR) class of immune receptors, thereby positively regulating plant immunity. SGT1 has also been found to be associated with the SKP1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. However, whether SGT1 targets immune repressors to coordinate plant immune activation remains elusive. In this study, we constructed a toolbox for TurboID- and split-TurboID-based proximity labeling (PL) assays in Nicotiana benthamiana and used the PL toolbox to explore the SGT1 interactome during pre- and post-immune activation. The comprehensive SGT1 interactome network we identified highlights a dynamic shift from proteins associated with plant development to those linked with plant immune responses. We found that SGT1 interacts with Necrotic Spotted Lesion 1 (NSL1), which negatively regulates salicylic acid-mediated defense by interfering with the nucleocytoplasmic trafficking of non-expressor of pathogenesis-related genes 1 (NPR1) during N NLR-mediated response to tobacco mosaic virus. SGT1 promotes the SCF-dependent degradation of NSL1 to facilitate immune activation, while salicylate-induced protein kinase-mediated phosphorylation of SGT1 further potentiates this process. Besides N NLR, NSL1 also functions in several other NLR-mediated immunity. Collectively, our study unveils the regulatory landscape of SGT1 and reveals a novel SGT1-NSL1 signaling module that orchestrates plant innate immunity.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1369-1391"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0