Molecular Plant最新文献_第9页

FERONIA: Organizing plant thermotolerance from the cell surface FERONIA：从细胞表面组织植物的耐热性

IF 27.5 1区生物学

Molecular Plant Pub Date : 2026-08-03 DOI: 10.1016/j.molp.2026.08.001

Muhammad Qadir,Shaowen Song,Feng Yu,Long Wang

引用次数: 0

Hijacking host deubiquitination system: A convergent evolutionary strategy for plant viruses to safeguard viral proteome stability. 劫持宿主去泛素化系统：植物病毒保护病毒蛋白质组稳定性的趋同进化策略。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-08-03 Epub Date: 2026-06-29 DOI: 10.1016/j.molp.2026.06.008

Zhaoxiong Jiang, Yi Li

引用次数: 0

GERAS: A Greek god and Mortal Kombat character regulates leaf senescence. GERAS：希腊神，真人快打中的角色，控制叶子衰老。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-08-03 Epub Date: 2026-07-02 DOI: 10.1016/j.molp.2026.06.009

Judy A Brusslan

引用次数: 0

PlantPTM: A deep learning framework integrating protein language models with multi-view features for predicting diverse post-translational modification sites in plants PlantPTM：一个深度学习框架，集成了蛋白质语言模型和多视图特征，用于预测植物中不同的翻译后修饰位点

IF 27.5 1区生物学

Molecular Plant Pub Date : 2026-08-03 DOI: 10.1016/j.molp.2026.08.002

Gensheng Dou,Kaiyuan Wang,Zhaohui Qin,Min Zhang,Wentian Zhang,Chunbo Miao,Junzhou Li,Zhen Chen

{"title":"PlantPTM: A deep learning framework integrating protein language models with multi-view features for predicting diverse post-translational modification sites in plants","authors":"Gensheng Dou,Kaiyuan Wang,Zhaohui Qin,Min Zhang,Wentian Zhang,Chunbo Miao,Junzhou Li,Zhen Chen","doi":"10.1016/j.molp.2026.08.002","DOIUrl":"https://doi.org/10.1016/j.molp.2026.08.002","url":null,"abstract":"Post-translational modifications (PTMs) play crucial regulatory roles in plants, orchestrating protein functions to maintain metabolic homeostasis, enable adaptation to dynamic environments, and regulate diverse cellular processes. Thus, the identification of PTM sites is essential for elucidating the mechanisms underlying plant growth, development, and stress responses. However, reliable and cost-effective computational approaches for predicting PTM sites in plants remain lacking. Here, we present PlantPTM, an integrated deep learning framework for predicting nine PTM types in plants. By combining protein language models (PLMs) with evolutionary information, PlantPTM demonstrates robust generalizability across a wide range of PTM types and plant species. Our method achieves state-of-the-art performance, with a mean AUROC of 0.8640 and a peak AUROC of 0.9699 across the nine PTM types. Notably, PlantPTM maintains strong performance even under low-data scenarios and unseen species. Extensive comparative benchmarks demonstrate that PlantPTM outperforms existing PTM prediction tools by an average of 15.46%, with improvements ranging from 2.90% to 19.08% over the best-performing tools for each PTM type. Furthermore, independent in-house MS data confirmed the accuracy of PlantPTM for ubiquitination, acetylation, and N-glycosylation sites, with all AUROC values exceeding 0.8. To facilitate PTM studies in plants, we provide the PlantPTM online service and source code freely available at https://ai4bio.online/PlantPTM and https://github.com/wky0422/PlantPTM, respectively.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"59 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148666040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A minimal four-enzyme suite enables biosynthesis of the C₂₀ diterpenoid alkaloid atisinium. 一个最小的四酶组使C20二萜生物碱Atisinium的生物合成。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-08-03 Epub Date: 2026-07-08 DOI: 10.1016/j.molp.2026.07.004

Jun Xie, Changyi Li, Xinye Kang, Yang Zou, Jingbo Li, Meng Li, Jiali Zhou, Jingyi Li, Naoki Kitaoka, Shilin Chen, Meirong Jia

{"title":"A minimal four-enzyme suite enables biosynthesis of the C20 diterpenoid alkaloid atisinium.","authors":"Jun Xie, Changyi Li, Xinye Kang, Yang Zou, Jingbo Li, Meng Li, Jiali Zhou, Jingyi Li, Naoki Kitaoka, Shilin Chen, Meirong Jia","doi":"10.1016/j.molp.2026.07.004","DOIUrl":"10.1016/j.molp.2026.07.004","url":null,"abstract":"Diterpenoid alkaloids (DAs) are pharmaceutically important metabolites and mainly occur in the Ranunculaceae. Despite their structural complexity, all DAs originate from C20 diterpenoid scaffolds, making the elucidation of C20-DA biosynthesis pivotal for understanding and engineering this entire alkaloid class. However, the molecular basis for DA core assembly has remained unresolved despite decades of research. Here, we define a minimal four-enzyme suite comprising three cytochrome P450 monooxygenases (CYP701A216, CYP71FH3, and CYP729G3) and one neo-functionalized short-chain dehydrogenase/reductase (AgDAS) that converts ent-atiserene into the C20 atisine-type alkaloid atisinium in Aconitum gymnandrum via an enzymatic-spontaneous-enzymatic process. Specifically, the multifunctional CYP701A216 and CYP71FH3 act in relay to generate the key intermediate ent-atiseren-19,20-dial, which undergoes spontaneous condensation with ethanolamine to form a hemiaminal. AgDAS then catalyzes atypical imine reduction to generate 15-deoxyatisinium bearing the characteristic atisine skeleton, and CYP729G3 mediates C15 hydroxylation to finalize atisinium biosynthesis. The physiological relevance of these enzymes was further investigated using virus-induced gene silencing in A. gymnandrum, demonstrating the critical role of AgDAS in this biosynthetic pathway. This compact enzyme module is highly conserved across Aconitum and Delphinium within the Ranunculaceae, pointing to an evolutionarily preserved core biosynthetic pathway. Collectively, our work establishes the complete molecular logic for DA scaffold assembly and its heterologous reconstitution in tobacco and yeast, opening routes to synthetic biology of medicinally relevant DAs.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1726-1741"},"PeriodicalIF":32.7,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence as an inverse problem: Diagnosing PETC-CBB limitations in dynamic environments. 荧光作为逆问题：诊断动态环境中PETC-CBB的局限性。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-08-03 Epub Date: 2026-07-09 DOI: 10.1016/j.molp.2026.07.006

Tim Nies, El-Hadji Malick Cisse, Anna Matuszyńska

引用次数: 0

Characterization of the entry steps in diterpenoid alkaloid biosynthesis. 二萜类生物碱生物合成进入步骤的表征。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-08-03 Epub Date: 2026-06-02 DOI: 10.1016/j.molp.2026.05.022

Garret P Miller, Lana Mutabdžija-Nedelcheva, Trine B Andersen, Imani Pascoe, Kathryn Van Winkle, Maryam Sabbaghan, Alexandre Bouillé, Tomáš Iliaš, Andrej Tekel, Tomáš Pluskal, Björn Hamberger

{"title":"Characterization of the entry steps in diterpenoid alkaloid biosynthesis.","authors":"Garret P Miller, Lana Mutabdžija-Nedelcheva, Trine B Andersen, Imani Pascoe, Kathryn Van Winkle, Maryam Sabbaghan, Alexandre Bouillé, Tomáš Iliaš, Andrej Tekel, Tomáš Pluskal, Björn Hamberger","doi":"10.1016/j.molp.2026.05.022","DOIUrl":"10.1016/j.molp.2026.05.022","url":null,"abstract":"The diterpenoid alkaloids are a group of specialized metabolites where the terpenoid and alkaloid classes intersect, and which are found primarily within the Aconitum and Delphinium genera. While there is considerable interest in these compounds for their wide range of bioactivities, their structural complexity poses a significant challenge for chemical synthesis. Biosynthesis offers an appealing alternative for production; however, little progress has been made toward elucidation of their biosynthetic pathways. In this study, we employed a comparative transcriptomics approach to identify six enzymatic steps in the biosynthesis of atisinium, conserved across both Delphinium grandiflorum and Aconitum plicatum. Key to this pathway is a reductase which selectively incorporates ethanolamine over ethylamine into the diterpenoid scaffold. While the majority of diterpenoid alkaloids contain an ethylamine moiety, we demonstrated through isotope labeling in Aconitum callus cultures and a computational metabolomics approach that ethanolamine is, unintuitively, the preferred source of nitrogen for these metabolites. Identification of these enzymes and production of a key intermediate in a heterologous host paves the way for biosynthetic production of this group of metabolites with promise for medicinal applications.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1711-1725"},"PeriodicalIF":32.7,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13533178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TMK1-mediated phosphorylation and destabilization of DA1 connects auxin signaling with seed size control in Arabidopsis. 在拟南芥中，生长素共受体tmk1介导的DA1磷酸化和失稳将生长素信号传导与种子大小控制联系起来。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-08-03 Epub Date: 2026-06-01 DOI: 10.1016/j.molp.2026.05.020

Anbin Wang, Leiying Zheng, Xindong Zhang, Weicheng Yang, Shichuang Yan, Wenyan Zhao, Li Yan, Tinghui Ge, Zhihai Chi, Xiahe Huang, Yongqiang Yu, Gang Gao, Yingchun Wang, Tongda Xu, Yunhai Li

{"title":"TMK1-mediated phosphorylation and destabilization of DA1 connects auxin signaling with seed size control in Arabidopsis.","authors":"Anbin Wang, Leiying Zheng, Xindong Zhang, Weicheng Yang, Shichuang Yan, Wenyan Zhao, Li Yan, Tinghui Ge, Zhihai Chi, Xiahe Huang, Yongqiang Yu, Gang Gao, Yingchun Wang, Tongda Xu, Yunhai Li","doi":"10.1016/j.molp.2026.05.020","DOIUrl":"10.1016/j.molp.2026.05.020","url":null,"abstract":"Auxin plays a central role in numerous developmental processes throughout the plant lifespan. Despite its well-established roles in plant growth, how auxin determines seed size and the molecular mechanism that links auxin signaling and seed size regulators still remain unclear. Here, we report that TMK1 (Transmembrane Kinase 1), a critical regulator of auxin signaling, acts maternally to regulate seed size and mediates auxin-promoted seed growth in Arabidopsis. TMK1 interacts with and phosphorylates the peptidase DA1, a key negative regulator of seed size, resulting in the destabilization of DA1. This process is stimulated by auxin, which not only promotes DA1 phosphorylation but also facilitates the accumulation of its target, the ubiquitin-specific protease UBP15, through TMK1. Genetic analyses further support that TMK1, DA1, and UBP15 act in the same pathway to control seed size. Thus, our findings reveal a novel mechanism by which auxin signaling controls seed size through TMK1-dependent phosphorylation and destabilization of DA1 in Arabidopsis, shedding new light on seed size improvement in crops.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1647-1662"},"PeriodicalIF":32.7,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148143970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the salt-tolerance code of Green Revolution cereals. 破解绿色革命谷物的耐盐密码。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-08-03 Epub Date: 2026-06-17 DOI: 10.1016/j.molp.2026.06.003

Pan Geng, Xiaofeng Fang

引用次数: 0

Orthologous synteny provides robust structural evidence for the ancestral angiosperm ε-WGD. 同源合性为被子植物祖先ε-WGD提供了强有力的结构证据。

IF 32.7 1区生物学

Molecular Plant Pub Date : 2026-08-03 Epub Date: 2026-06-19 DOI: 10.1016/j.molp.2026.06.006

Ren-Gang Zhang, Martin A Lysak, Hong-Yun Shang, Yuan-Nian Jiao, Yong-Peng Ma

引用次数: 0