Molecular Plant最新文献_第8页

The miR172a-SNB module orchestrates both induced and adult-plant resistance to multiple diseases via MYB30-mediated lignin accumulation in rice. miR172a-SNB模块通过myb30介导的木质素积累协调水稻对多种疾病的诱导抗性和成体抗性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-01-06 Epub Date: 2024-11-30 DOI: 10.1016/j.molp.2024.11.015

He Wang, Zhe-Xu Wang, Hong-Yuan Tian, Yu-Long Zeng, Hao Xue, Wan-Ting Mao, Lu-Yue Zhang, Jun-Ni Chen, Xiang Lu, Yong Zhu, Guo-Bang Li, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Pei-Zhou Xu, Xiao-Qiong Chen, Wei-Tao Li, Xian-Jun Wu, Wen-Ming Wang, Yan Li

{"title":"The miR172a-SNB module orchestrates both induced and adult-plant resistance to multiple diseases via MYB30-mediated lignin accumulation in rice.","authors":"He Wang, Zhe-Xu Wang, Hong-Yuan Tian, Yu-Long Zeng, Hao Xue, Wan-Ting Mao, Lu-Yue Zhang, Jun-Ni Chen, Xiang Lu, Yong Zhu, Guo-Bang Li, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Pei-Zhou Xu, Xiao-Qiong Chen, Wei-Tao Li, Xian-Jun Wu, Wen-Ming Wang, Yan Li","doi":"10.1016/j.molp.2024.11.015","DOIUrl":"10.1016/j.molp.2024.11.015","url":null,"abstract":"Plants mount induced resistance and adult-plant resistance against different pathogens throughout the whole growth period. Rice production faces threats from multiple major diseases, including rice blast, sheath blight, and bacterial leaf blight. Here, we report that the miR172a-SNB-MYB30 module regulates both induced and adult-plant resistance to these three major diseases via lignification in rice. Mechanistically, pathogen infections induce the expression of miR172a, which downregulates the transcription factor SNB to release its suppression of MYB30, leading to an increase in lignin biosynthesis and disease resistance throughout the whole growth period. Moreover, expression levels of miR172a and MYB30 gradually increase and are consistently correlated with lignin contents and disease resistance during rice development, reaching a peak at full maturity, whereas SNB RNA levels are negatively correlated with lignin contents and disease resistance, indicating the involvement of the miR172a-SNB-MYB30 module in adult-plant resistance. The functional domain of SNB protein and its binding sites in the MYB30 promoter are highly conserved among more than 4000 rice accessions, while abnormal expression of miR172a, SNB, or MYB30 compromises yield traits, suggesting artificial selection of the miR172a-SNB-MYB30 module during rice domestication. Taken together, these results reveal a novel role for a conserved miRNA-regulated module that contributes significantly to induced and adult-plant resistance against multiple pathogens by increasing lignin accumulation, deepening our understanding of broad-spectrum resistance and adult-plant resistance.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"59-75"},"PeriodicalIF":17.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ALFIN-like proteins link histone H3K4me3 to H2A ubiquitination and coordinate diverse chromatin modifications in Arabidopsis. ALFIN 样蛋白将组蛋白 H3K4me3 与 H2A 泛素化联系起来，并协调拟南芥中的多种染色质修饰。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-01-06 Epub Date: 2024-12-11 DOI: 10.1016/j.molp.2024.12.007

Xiao-Min Su, Dan-Yang Yuan, Na Liu, Zhao-Chen Zhang, Minqi Yang, Lin Li, She Chen, Yue Zhou, Xin-Jian He

{"title":"ALFIN-like proteins link histone H3K4me3 to H2A ubiquitination and coordinate diverse chromatin modifications in Arabidopsis.","authors":"Xiao-Min Su, Dan-Yang Yuan, Na Liu, Zhao-Chen Zhang, Minqi Yang, Lin Li, She Chen, Yue Zhou, Xin-Jian He","doi":"10.1016/j.molp.2024.12.007","DOIUrl":"10.1016/j.molp.2024.12.007","url":null,"abstract":"Trimethylation of histone H3K4 (H3K4me3) is widely distributed at numerous actively transcribed protein-coding genes throughout the genome. However, the interplay between H3K4me3 and other chromatin modifications in plants remains poorly understood. In this study, we show that the Arabidopsis thaliana ALFIN-LIKE (AL) proteins contain a C-terminal PHD finger capable of binding to H3K4me3 and a PHD-associated AL (PAL) domain that interacts with components of the Polycomb repressive complex 1, thereby facilitating H2A ubiquitination (H2Aub) at H3K4me3-enriched genes throughout the genome. Furthermore, we demonstrate that loss of function of SDG2, encoding a key histone H3K4 methyltransferase, leads to a reduction in H3K4me3 level, which subsequently causes a genome-wide decrease in H2Aub, revealing a strong association between H3K4me3 and H2Aub. Finally, we discover that the PAL domain of AL proteins interacts with various other chromatin-related proteins or complexes, including those involved in regulating H2A.Z deposition, H3K27me3 demethylation, histone deacetylation, and chromatin accessibility. Our genome-wide analysis suggests that the AL proteins play a crucial role in coordinating H3K4me3 with multiple other chromatin modifications across the genome.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"130-150"},"PeriodicalIF":17.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How ignoring market-informed crop varietal development fuels food price volatility. 忽视以市场为导向的作物品种开发是如何加剧粮食价格波动的？

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-01-06 Epub Date: 2024-12-12 DOI: 10.1016/j.molp.2024.12.008

Ganesh Alagarasan

引用次数: 0

A new gene for restoring wild abortive-type cytoplasmic male sterility in rice. 恢复水稻野生败育型细胞质雄性不育的新基因

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-09 DOI: 10.1016/j.molp.2024.11.004

Xiaoming Zheng, Jian Sun, Cheng Cheng, Qian Qian

引用次数: 0

A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis. 拟南芥中由替代剪接产生的诱饵受体可微调细胞分裂素信号。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-05 DOI: 10.1016/j.molp.2024.11.001

Michaela Králová, Ivona Kubalová, Jakub Hajný, Karolina Kubiasová, Karolína Vagaská, Zengxiang Ge, Michelle Gallei, Hana Semerádová, Anna Kuchařová, Martin Hönig, Aline Monzer, Martin Kovačik, Jiří Friml, Ondřej Novák, Eva Benková, Yoshihisa Ikeda, David Zalabák

{"title":"A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis.","authors":"Michaela Králová, Ivona Kubalová, Jakub Hajný, Karolina Kubiasová, Karolína Vagaská, Zengxiang Ge, Michelle Gallei, Hana Semerádová, Anna Kuchařová, Martin Hönig, Aline Monzer, Martin Kovačik, Jiří Friml, Ondřej Novák, Eva Benková, Yoshihisa Ikeda, David Zalabák","doi":"10.1016/j.molp.2024.11.001","DOIUrl":"10.1016/j.molp.2024.11.001","url":null,"abstract":"Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants. Cytokinins, a class of plant hormones, regulate cell division and meristem maintenance. The cytokinin signaling pathway is well established in the model plant Arabidopsisthaliana. Several negative feedback mechanisms, tightly controlling cytokinin signaling output, have been described previously. In this study, we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1. A novel splicing variant named CRE1int7 results from seventh intron retention, introducing a premature termination codon in the transcript. We showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction. CRE1int7 can bind cytokinin but cannot activate the downstream cascade. We present a novel negative feedback mechanism of the cytokinin signaling pathway, facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding. Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels. CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1850-1865"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems. 合成生物学系统揭示了角草 Rubiscos 独特的生物生成和动力学过程。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-02 DOI: 10.1016/j.molp.2024.10.013

Zhen Guo Oh, Tanner Ashton Robison, Dan Hong Loh, Warren Shou Leong Ang, Jediael Zheng Ying Ng, Fay-Wei Li, Laura Helen Gunn

{"title":"Unique biogenesis and kinetics of hornwort Rubiscos revealed by synthetic biology systems.","authors":"Zhen Guo Oh, Tanner Ashton Robison, Dan Hong Loh, Warren Shou Leong Ang, Jediael Zheng Ying Ng, Fay-Wei Li, Laura Helen Gunn","doi":"10.1016/j.molp.2024.10.013","DOIUrl":"10.1016/j.molp.2024.10.013","url":null,"abstract":"Hornworts are the only land plants that employ a pyrenoid to optimize Rubisco's CO2 fixation, yet hornwort Rubisco remains poorly characterized. Here we assembled the hornwort Anthoceros agrestis Rubisco (AaRubisco) using the Arabidopsis thaliana SynBio expression system and observed the formation of stalled intermediates, prompting us to develop a new SynBio system with A. agrestis cognate chaperones. We successfully assembled AaRubisco and Rubisco from three other hornwort species. Unlike A. thaliana Rubisco, AaRubisco assembly is not dependent on RbcX or Raf2. Kinetic characterization reveals that hornwort Rubiscos exhibit a range of catalytic rates (3-10 s-1), but with similar affinity (∼30 μM) and specificity (∼70) for CO2. These results suggest that hornwort Rubiscos do not comply with the long-held canonical catalytic trade-off observed in other land plants, providing experimental support that Rubisco kinetics may be phylogenetically constrained. Unexpectedly, we observed a 50% increase in AaRubisco catalytic rates when RbcX was removed from our SynBio system, without any reduction in specificity. Structural biology, biochemistry, and proteomic analysis suggest that subtle differences in Rubisco large-subunit interactions, when RbcX is absent during biogenesis, increases the accessibility of active sites and catalytic turnover rate. Collectively, this study uncovered a previously unknown Rubisco kinetic parameter space and provides a SynBio chassis to expand the survey of other Rubisco kinetics. Our discoveries will contribute to developing new approaches for engineering Rubisco with superior kinetics.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1833-1849"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H₂O₂ sulfenylates CHE to activate systemic salicylic acid synthesis and ignite systemic acquired resistance. H2O2 亚磺酰化 CHE，激活全身水杨酸合成，点燃全身获得性抵抗。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-17 DOI: 10.1016/j.molp.2024.10.007

Kaihuai Li, Cheng Li, Daowen Wang, Fengquan Liu, Zheng Qing Fu

引用次数: 0

Upland rice retains genetic elements conferring drought adaptation. 陆地水稻保留了赋予干旱适应性的遗传元素。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-11 DOI: 10.1016/j.molp.2024.10.006

Zeqi Li, Yi Zhang, Hao Du

引用次数: 0

Natural variation in maize ZmLecRK1 fine-tunes co-receptor interactions to boost immunity. 玉米 ZmLecRK1 的自然变异可微调共受体的相互作用，从而增强免疫力。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-30 DOI: 10.1016/j.molp.2024.10.012

Miguel-Ángel Torres, Lucía Jordá

引用次数: 0

SCOOP10 and SCOOP12 peptides act through MIK2 receptor-like kinase to antagonistically regulate Arabidopsis leaf senescence. SCOOP10 和 SCOOP12 肽通过 MIK2 受体样激酶拮抗拟南芥叶片衰老。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-28 DOI: 10.1016/j.molp.2024.10.010

Zhenbiao Zhang, Nora Gigli-Bisceglia, Wei Li, Saijie Li, Jie Wang, Junfeng Liu, Christa Testerink, Yongfeng Guo

{"title":"SCOOP10 and SCOOP12 peptides act through MIK2 receptor-like kinase to antagonistically regulate Arabidopsis leaf senescence.","authors":"Zhenbiao Zhang, Nora Gigli-Bisceglia, Wei Li, Saijie Li, Jie Wang, Junfeng Liu, Christa Testerink, Yongfeng Guo","doi":"10.1016/j.molp.2024.10.010","DOIUrl":"10.1016/j.molp.2024.10.010","url":null,"abstract":"Leaf senescence plays a critical role in a plant's overall reproductive success due to its involvement in nutrient remobilization and allocation. However, our current understanding of the molecular mechanisms controlling leaf senescence remains limited. In this study, we show that the receptor-like kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) functions as a negative regulator of leaf senescence. We found that the SERINE-RICH ENDOGENOUS PEPTIDE 12, previously known to physically interact with MIK2, competes with SCOOP10 to regulate MIK2-dependent leaf senescence. We observed that increased expression of SCOOP10 or the application of exogenous SCOOP10 peptides accelerated leaf senescence in a MIK2-dependent manner. Conversely, SCOOP12 acted as a suppressor of MIK2-dependent leaf senescence regulation. Biochemical assays showed that SCOOP12 enhances while SCOOP10 diminishes MIK2 phosphorylation. Thus, the SCOOP12-MIK2 module might function antagonistically on SCOOP10-MIK2 signaling at late senescing stages, allowing for fine-tuned modulation of the leaf senescence process. Our study sheds light on the complex mechanisms underlying leaf senescence and provides valuable insights into the interplay between receptors, peptides, and the regulation of plant senescence.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1805-1819"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0