Molecular Plant最新文献_第8页

The plant-specific protein IQD22 interacts with calcium sensors to activate anaerobic respiration during hypoxia in Arabidopsis. 植物特异性蛋白IQD22与钙传感器相互作用，在拟南芥缺氧时激活厌氧呼吸。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-08-04 Epub Date: 2025-07-12 DOI: 10.1016/j.molp.2025.07.005

Shanshan Zhao, Qin-Fang Chen, Li Chen, Ying Zhou, Ke Liao, Fengzhu Wang, Xue Zhang, Moxian Chen, Ruo-Han Xie, Shi Xiao

{"title":"The plant-specific protein IQD22 interacts with calcium sensors to activate anaerobic respiration during hypoxia in Arabidopsis.","authors":"Shanshan Zhao, Qin-Fang Chen, Li Chen, Ying Zhou, Ke Liao, Fengzhu Wang, Xue Zhang, Moxian Chen, Ruo-Han Xie, Shi Xiao","doi":"10.1016/j.molp.2025.07.005","DOIUrl":"10.1016/j.molp.2025.07.005","url":null,"abstract":"Louis Pasteur first reported that living cells switch from aerobic to anaerobic metabolism under low-oxygen conditions, but the underlying regulatory mechanism remains to be fully elucidated. ALCOHOL DEHYDROGENASE 1 (ADH1) encodes a key enzyme in ethanolic fermentation and is upregulated under hypoxia. In this study, we searched for Arabidopsis thaliana mutants with defects in hypoxia-induced ADH1 expression and identified the IQ DOMAIN containing protein 22 (IQD22) as a crucial regulator of ADH1-mediated hypoxia tolerance. The iqd22 mutant plants were hypersensitive to submergence and hypoxic stress as compared with the wild-type plants, whereas IQD22 overexpressors were more tolerant. We showed that under hypoxia, IQD22 enhances the interaction between the calcium-dependent protein kinase CPK12 and the ETHYLENE RESPONSE FACTOR (ERF)-VII-type transcription factor RELATED TO AP2.12 (RAP2.12) to upregulate hypoxia-responsive genes, including ADH1. Moreover, we found that IQD22 interacts with calmodulins (CaMs) in vivo and facilitates their association with ADH1, stimulating its abundance in response to hypoxia. Metabolic profiling revealed that hypoxia causes significant increase in glycolytic metabolites but greatly lower ethanol accumulation in the iqd22-2 mutant. Genetic analysis showed that disruption of ADH1 suppresses the improved hypoxia-tolerance phenotype of IQD22 overexpressors. Taken together, these results indicate that IQD22 functions in the CaM-ADH1 and CPK12-RAP2.12 regulatory modules, which coordinately mediate calcium-dependent activation of anaerobic respiration to control metabolic flux during hypoxia.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1330-1350"},"PeriodicalIF":24.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The RING-finger ubiquitin E3 ligase RFEL1 targets wheat NPR3 for degradation to confer broad-spectrum resistance against biotrophic fungal pathogens. 环指泛素E3连接酶RFEL1靶向小麦NPR3降解，赋予对生物营养真菌病原体的广谱抗性。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-08-04 Epub Date: 2025-07-15 DOI: 10.1016/j.molp.2025.07.008

Liuhui Qiao, Kunpu Zhang, Jinyan Li, Ziming Zhang, Xiao Sun, Huiyun Liu, Ziyue Li, Nannan Ni, Ximei Ma, Jianhui Zhao, Guangwei Li, Xiaohuan Jin, Jibin Xiao, Wenming Zheng, Daowen Wang, Zheng Qing Fu, Huan Wang

{"title":"The RING-finger ubiquitin E3 ligase RFEL1 targets wheat NPR3 for degradation to confer broad-spectrum resistance against biotrophic fungal pathogens.","authors":"Liuhui Qiao, Kunpu Zhang, Jinyan Li, Ziming Zhang, Xiao Sun, Huiyun Liu, Ziyue Li, Nannan Ni, Ximei Ma, Jianhui Zhao, Guangwei Li, Xiaohuan Jin, Jibin Xiao, Wenming Zheng, Daowen Wang, Zheng Qing Fu, Huan Wang","doi":"10.1016/j.molp.2025.07.008","DOIUrl":"10.1016/j.molp.2025.07.008","url":null,"abstract":"Broad-spectrum resistance (BSR) is highly sought after for the effective management of crop diseases. However, genes suitable for developing BSR remain scarce. In this study, we demonstrate the development of BSR to wheat yellow rust (YR), powdery mildew (PM), and leaf rust (LR) diseases elicited by three biotrophic fungal pathogens using a newly defined module, namely, RFEL1-NPR3. RFEL1 is an active RING-finger E3 ubiquitin ligase identified in diploid and polyploid wheat species, which ubiquitinates and promotes the degradation of wheat NPR3 (TaNPR3), an important negative immune regulator conserved in higher plants, via the 26S proteasome system. Downregulation of TaNPR3 by either overexpressing RFEL1 or knocking out TaNPR3 confers strong resistance against four different YR races as well as the PM and LR diseases without adverse effects on wheat growth and yield traits. Notably, the enhanced disease resistance exhibited by RFEL1-overexpressing and TaNPR3-knockout lines is correlated with increased expression of defense related genes and elevated stability of NPR1, a pivotal positive regulator of plant immune signaling. Our findings underscore the importance of ubiquitination-dependent NPR3 degradation in plant immunity and advocate for the application of the RFEL1-NPR3 module in engineering BSR against biotrophic fungal pathogens in wheat and other crops.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1351-1368"},"PeriodicalIF":24.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methionine metabolism and biofortification in maize: Regulatory networks and genetic strategies. 玉米蛋氨酸代谢和生物强化：调控网络和遗传策略。

IF 24.1 1区生物学

Molecular Plant Pub Date : 2025-08-04 Epub Date: 2025-06-25 DOI: 10.1016/j.molp.2025.06.014

Rachel Amir

引用次数: 0

A developmental switch controls cell-to-cell transport in roots via pectin-linked plasmodesmata changes 一个发育开关通过果胶连接的胞间连丝改变控制根中细胞间的运输

IF 27.5 1区生物学

Molecular Plant Pub Date : 2025-07-17 DOI: 10.1016/j.molp.2025.07.004

Léa Jacquier, Celeste Aurora Fiorenza, Kevin Robe, Jian-Pu Han, Alexandra Schmitt, Fabienne Cléard, Christelle Fuchs, Priya Ramakrishna, Sylvain Loubéry, Lothar Kalmbach, Linnka Lefebvre-Legendre, Marie Barberon

{"title":"A developmental switch controls cell-to-cell transport in roots via pectin-linked plasmodesmata changes","authors":"Léa Jacquier, Celeste Aurora Fiorenza, Kevin Robe, Jian-Pu Han, Alexandra Schmitt, Fabienne Cléard, Christelle Fuchs, Priya Ramakrishna, Sylvain Loubéry, Lothar Kalmbach, Linnka Lefebvre-Legendre, Marie Barberon","doi":"10.1016/j.molp.2025.07.004","DOIUrl":"https://doi.org/10.1016/j.molp.2025.07.004","url":null,"abstract":"Cell-to-cell communication is fundamental to multicellular life. In plants, plasmodesmata - cytoplasmic channels - enable molecular transport between adjacent cells. In roots, this transport is predicted to play a central role in nutrient acquisition and delivery across the multiple cell layers that compose the root. In this study, we demonstrate that plasmodesmatal transport persists in fully differentiated roots, despite the formation of apoplastic barriers such as Casparian strips and suberin lamellae in the endodermis. This persistence highlights plasmodesmata as a critical pathway for intercellular transport in mature roots. We also uncovered a developmental switch in plasmodesmata function: while transport is bidirectional in young roots, it becomes unidirectional towards the vasculature in differentiated roots. Through a genetic screen, we identified mutants with disrupted directionality, exhibiting persistent bidirectional transport. These mutants showed enlarged plasmodesmata apertures caused by defects in pectin composition and cell wall organization, highlighting the critical role of pectin in plasmodesmata formation and function. Our findings reveal how plasmodesmata-mediated transport is dynamically regulated during root development and provide new insights into the cellular mechanisms that govern intercellular communication in plants.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":"13 1","pages":""},"PeriodicalIF":27.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144664828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel NF-Ys-QT12-IRE1 module controlling grain quality and yield thermotolerance in rice. 调控水稻籽粒品质和产量耐热性的新型NF-Ys-QT12-IRE1模块。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-07-07 Epub Date: 2025-05-13 DOI: 10.1016/j.molp.2025.05.007

Bowen Yang, Yongyao Xie, Yaoguang Liu

引用次数: 0

The transcription factor MYB44 suppresses starch synthesis to negatively regulate grain weight and yield in wheat and rice. 转录因子MYB44抑制淀粉合成，负调控小麦和水稻的粒重和产量。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-07-07 Epub Date: 2025-06-18 DOI: 10.1016/j.molp.2025.06.007

Yunchuan Liu, Mingming Wang, Yaojia Wang, Haixia Liu, Wei Xi, David Seung, Xiaolu Wang, Lei Zhuang, Huifang Li, Tian Li, Hongxia Liu, Jian Hou, Xu Liu, Chenyang Hao, Xueyong Zhang

{"title":"The transcription factor MYB44 suppresses starch synthesis to negatively regulate grain weight and yield in wheat and rice.","authors":"Yunchuan Liu, Mingming Wang, Yaojia Wang, Haixia Liu, Wei Xi, David Seung, Xiaolu Wang, Lei Zhuang, Huifang Li, Tian Li, Hongxia Liu, Jian Hou, Xu Liu, Chenyang Hao, Xueyong Zhang","doi":"10.1016/j.molp.2025.06.007","DOIUrl":"10.1016/j.molp.2025.06.007","url":null,"abstract":"Starch is the primary storage compound in wheat grains and is essential for both flour quality and grain weight. In this study, we identified TaMYB44, an R2R3-MYB transcription factor gene that controls starch content in wheat grains, through a genome-wide association study. The TaMYB44 homoeologs were predominantly expressed in developing grains, with peak levels observed 10 days after pollination. Functional analyses revealed that TaMYB44 acts as a negative regulator of starch synthesis in the endosperm, limiting grain size by repressing starch synthesis-related genes and modulating secondary metabolism. Knockout mutants of TaMYB44 exhibited significantly increased starch accumulation, larger grain size, and improved yield stability across diverse growing environments. Furthermore, we discovered that TaWDR1 interacts with TaMYB44, alleviating its repressive effects to restore starch synthesis and enhance grain weight. Notably, the functions of MYB44 appear to be partially conserved between wheat and rice, underscoring its potential as a target for genetic improvement. Our findings offer valuable insights into the transcriptional regulation of starch synthesis and provide genetic resources for enhancing grain yield in wheat and rice.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1193-1209"},"PeriodicalIF":17.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of the CNGC2-CNGC4 channel complex by P2K1-mediated phosphorylation links extracellular ATP perception to calcium signaling in plant immunity. 通过p2k1介导的磷酸化激活CNGC2-CNGC4通道复合物，将细胞外ATP感知与植物免疫中的钙信号传导联系起来。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-07-07 Epub Date: 2025-06-04 DOI: 10.1016/j.molp.2025.06.001

Yujia Sun, Lei Gao, Yiping Han, Changxin Feng, Zebin Liu, Chunyan Li, Yuxin Dong, Shuxin Yin, Laihao Liu, Ming Yang, Qi Niu, Dongdong Kong, Liangyu Liu, Jingbo Zhang, Wang Tian, Sheng Luan, Legong Li, Congcong Hou

{"title":"Activation of the CNGC2-CNGC4 channel complex by P2K1-mediated phosphorylation links extracellular ATP perception to calcium signaling in plant immunity.","authors":"Yujia Sun, Lei Gao, Yiping Han, Changxin Feng, Zebin Liu, Chunyan Li, Yuxin Dong, Shuxin Yin, Laihao Liu, Ming Yang, Qi Niu, Dongdong Kong, Liangyu Liu, Jingbo Zhang, Wang Tian, Sheng Luan, Legong Li, Congcong Hou","doi":"10.1016/j.molp.2025.06.001","DOIUrl":"10.1016/j.molp.2025.06.001","url":null,"abstract":"Extracellular adenosine triphosphate (eATP) functions as a damage-associated molecular pattern in plant immunity. P2K1, a purinergic receptor with a cytoplasmic serine/threonine kinase domain, initiates ATP-responsive signaling cascades characterized by a rapid spike in cytosolic Ca²⁺, which acts as a critical second messenger. In this study, we identified the cyclic nucleotide-gated channel complex CNGC2-CNGC4 as essential for eATP-induced calcium signaling and bacterial resistance in plants. A biochemical link between eATP perception and CNGC2-CNGC4 function was established by demonstrating the physical association between the channel complex and the eATP receptor P2K1 at the plasma membrane. Furthermore, we discovered that P2K1 phosphorylates the CNGC2 subunit of the CNGC2-CNGC4 channel in response to eATP, establishing a phosphorylation-dependent mechanism that connects eATP perception to calcium influx. Through AlphaFold-Multimer prediction, electrophysiological assay, and genetic analysis, we identified serine residues S705 and S718 in CNGC2 as the key phosphorylation sites mediating P2K1-dependent channel activation and eATP-triggered immunity. Notably, P2K1 selectively phosphorylates CNGC2, in contrast to BIK1 that phosphorylates CNGC4 during pathogen-associated molecular pattern-triggered immunity. Together, these findings indicate that the CNGC2-CNGC4 channel complex serves as a core component of calcium-dependent plant immunity, with distinct kinases phosphorylating different subunits in response to specific immune elicitors.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1130-1142"},"PeriodicalIF":17.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA demethylase augments RNA-directed DNA methylation by enhancing CLSY gene expression in maize and Arabidopsis. DNA去甲基化酶通过增强玉米和拟南芥CLSY基因的表达来增强rna导向的DNA甲基化。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-07-07 Epub Date: 2025-06-07 DOI: 10.1016/j.molp.2025.06.002

Qiang Xu, Qiuxin Kan, Zhixiang Luo, Qiang Zhang, Liang Dong, Nathan M Springer, Qing Li

{"title":"DNA demethylase augments RNA-directed DNA methylation by enhancing CLSY gene expression in maize and Arabidopsis.","authors":"Qiang Xu, Qiuxin Kan, Zhixiang Luo, Qiang Zhang, Liang Dong, Nathan M Springer, Qing Li","doi":"10.1016/j.molp.2025.06.002","DOIUrl":"10.1016/j.molp.2025.06.002","url":null,"abstract":"DNA methylation and demethylation activities are coordinated to maintain DNA methylation patterns. However, the interplay between them remains to be explored. In this study, we found that loss of DNA demethylase in maize results in a significant decrease in CHH methylation, a hallmark of RNA-directed DNA methylation (RdDM). Further analyses suggested that this is caused by reduced expression in the DNA demethylase mutant of three CLASSY (CLSY) genes, which encode chromatin remodelers controlling small RNA production. Series of molecular assays confirm that the expression of three maize CLSY genes is sensitive to the DNA methylation levels at their promoter regions, which are regulated by both the DNA demethylase and the RdDM pathway. Moreover, we revealed that Arabidopsis DNA demethylase mutants also show decreased CHH methylation and reduced expression of CLSY1, one of four CLSY genes in Arabidopsis. Similar to the observations in maize, the expression of CLSY1 is associated with DNA methylation levels of its promoter that is targeted by both DNA demethylase and RdDM pathways. Taken together, these results suggest a conserved interplay between DNA demethylation and RdDM pathways, revealing a mechanism to maintain the homeostasis of DNA methylation levels across plants.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1158-1170"},"PeriodicalIF":17.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylation-activated G protein signaling stabilizes TCP14 and JAZ3 to repress JA signaling and enhance plant immunity. 磷酸化激活的g蛋白信号稳定TCP14和JAZ3，抑制JA信号，增强植物免疫力。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-07-07 Epub Date: 2025-07-04 DOI: 10.1016/j.molp.2025.06.004

Haiyan Jia, Natalie Hewitt, Lucía Jordá, Tigran M Abramyan, Josh Tolliver, Janice L Jones, Kinya Nomura, Jing Yang, Sheng-Yang He, Alexander Tropsha, Antonio Molina, Henrik G Dohlman, Alan M Jones

{"title":"Phosphorylation-activated G protein signaling stabilizes TCP14 and JAZ3 to repress JA signaling and enhance plant immunity.","authors":"Haiyan Jia, Natalie Hewitt, Lucía Jordá, Tigran M Abramyan, Josh Tolliver, Janice L Jones, Kinya Nomura, Jing Yang, Sheng-Yang He, Alexander Tropsha, Antonio Molina, Henrik G Dohlman, Alan M Jones","doi":"10.1016/j.molp.2025.06.004","DOIUrl":"10.1016/j.molp.2025.06.004","url":null,"abstract":"The plant hormones salicylic acid (SA) and jasmonic acid (JA) act in mutual negative-feedback regulation to balance plant growth-defense trade-off. Heterotrimeric Gα-Gβ-Gγ proteins are hubs that regulate defense signaling. In Arabidopsis, the Gα (GPA1) and Gβ (AGB1) subunits are required for defense against biotrophic and necrotrophic pathogens; however, the upstream and downstream molecular mechanisms underlying G protein-mediated defense remain largely unclear. In this study, we found that G proteins are primarily negative regulators of JA signaling in response to pathogen attack. Both TCP14 and JAZs are transcriptional regulators in the JA pathways. We revealed that GPA1 interacts with TCP14 within nuclear foci, and AGB1 interacts with TCP14 and most of JAZ regulators, including JAZ3. Mechanistically, GPA1 slows the proteasomal degradation of the G protein-TCP14-JAZ3 complex, a process that is normally promoted by JA and the bacterial virulence effector HopBB1, thus boosting SA-based defense. In turn, GPA1 activity is regulated by JA-induced phosphorylation at a conserved residue located near the nucleotide-binding pocket and other residues within the N-terminal α helix. The phosphomimic mutations do not affect GTP binding or hydrolysis but enhance GPA1 interaction with TCP14 and JAZ3, thereby preventing their degradation. This newly discovered phosphorylation-dependent mechanism of de-sequestering G protein partners to modulate transcriptional regulation may extend to both yeast and human cells.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1171-1192"},"PeriodicalIF":17.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potatome: Harnessing natural variation of potatoes. 马铃薯-利用马铃薯的自然变异。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2025-07-07 Epub Date: 2025-05-23 DOI: 10.1016/j.molp.2025.05.008

Delphine Van Inghelandt, Benjamin Stich

引用次数: 0