Molecular Plant最新文献_第8页

Single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in St. John's wort. 单细胞 RNA 测序有助于阐明圣约翰草中抗抑郁剂金丝桃素的完整生物合成过程。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-12 DOI: 10.1016/j.molp.2024.08.003

Song Wu, Ana Luisa Malaco Morotti, Jun Yang, Ertao Wang, Evangelos C Tatsis

{"title":"Single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in St. John's wort.","authors":"Song Wu, Ana Luisa Malaco Morotti, Jun Yang, Ertao Wang, Evangelos C Tatsis","doi":"10.1016/j.molp.2024.08.003","DOIUrl":"10.1016/j.molp.2024.08.003","url":null,"abstract":"Hyperforin is the compound responsible for the effectiveness of St. John's wort (Hypericum perforatum) as an antidepressant, but its complete biosynthetic pathway remains unknown. Gene discovery based on co-expression analysis of bulk RNA-sequencing data or genome mining failed to discover the missing steps in hyperforin biosynthesis. In this study, we sequenced the 1.54-Gb tetraploid H. perforatum genome assembled into 32 chromosomes with the scaffold N50 value of 42.44 Mb. By single-cell RNA sequencing, we identified a type of cell, \"Hyper cells\", wherein hyperforin biosynthesis de novo takes place in both the leaves and flowers. Through pathway reconstitution in yeast and tobacco, we identified and characterized four transmembrane prenyltransferases (HpPT1-4) that are localized at the plastid envelope and complete the hyperforin biosynthetic pathway. The hyperforin polycyclic scaffold is created by a reaction cascade involving an irregular isoprenoid coupling and a tandem cyclization. Our findings reveal how and where hyperforin is biosynthesized, enabling synthetic-biology reconstitution of the complete pathway. Thus, this study not only deepens our comprehension of specialized metabolism at the cellular level but also provides strategic guidance for elucidation of the biosynthetic pathways of other specializied metabolites in plants.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1439-1457"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulation of photosensory and circadian signaling restricts phenotypic plasticity in response to changing environmental conditions in Arabidopsis. 操纵光感和昼夜节律信号可限制拟南芥对环境条件变化的表型可塑性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-15 DOI: 10.1016/j.molp.2024.07.007

Martin William Battle, Scott Fraser Ewing, Cathryn Dickson, Joseph Obaje, Kristen N Edgeworth, Rebecca Bindbeutel, Rea L Antoniou-Kourounioti, Dmitri A Nusinow, Matthew Alan Jones

{"title":"Manipulation of photosensory and circadian signaling restricts phenotypic plasticity in response to changing environmental conditions in Arabidopsis.","authors":"Martin William Battle, Scott Fraser Ewing, Cathryn Dickson, Joseph Obaje, Kristen N Edgeworth, Rebecca Bindbeutel, Rea L Antoniou-Kourounioti, Dmitri A Nusinow, Matthew Alan Jones","doi":"10.1016/j.molp.2024.07.007","DOIUrl":"10.1016/j.molp.2024.07.007","url":null,"abstract":"Plants exploit phenotypic plasticity to adapt their growth and development to prevailing environmental conditions. Interpretation of light and temperature signals is aided by the circadian system, which provides a temporal context. Phenotypic plasticity provides a selective and competitive advantage in nature but is obstructive during large-scale, intensive agricultural practices since economically important traits (including vegetative growth and flowering time) can vary widely depending on local environmental conditions. This prevents accurate prediction of harvesting times and produces a variable crop. In this study, we sought to restrict phenotypic plasticity and circadian regulation by manipulating signaling systems that govern plants' responses to environmental signals. Mathematical modeling of plant growth and development predicted reduced plant responses to changing environments when circadian and light signaling pathways were manipulated. We tested this prediction by utilizing a constitutively active allele of the plant photoreceptor phytochrome B, along with disruption of the circadian system via mutation of EARLY FLOWERING3. We found that these manipulations produced plants that are less responsive to light and temperature cues and thus fail to anticipate dawn. These engineered plants have uniform vegetative growth and flowering time, demonstrating how phenotypic plasticity can be limited while maintaining plant productivity. This has significant implications for future agriculture in both open fields and controlled environments.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1458-1471"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dose-dependent bimodal switch by homologous Aux/IAA transcriptional repressors. 同源 Aux/IAA 转录抑制因子的剂量依赖性双模切换。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-08-06 DOI: 10.1016/j.molp.2024.07.014

Hyung-Taeg Cho, Minsu Lee, Hee-Seung Choi, Kwang-Ho Maeng, Kyeonghoon Lee, Ha-Yeon Lee, Anindya Ganguly, Hoonyoung Park, Chang-Hoi Ho

{"title":"A dose-dependent bimodal switch by homologous Aux/IAA transcriptional repressors.","authors":"Hyung-Taeg Cho, Minsu Lee, Hee-Seung Choi, Kwang-Ho Maeng, Kyeonghoon Lee, Ha-Yeon Lee, Anindya Ganguly, Hoonyoung Park, Chang-Hoi Ho","doi":"10.1016/j.molp.2024.07.014","DOIUrl":"10.1016/j.molp.2024.07.014","url":null,"abstract":"Combinatorial interactions between different regulators diversify and enrich the chance of transcriptional regulation in eukaryotic cells. However, a dose-dependent functional switch of homologous transcriptional repressors has rarely been reported. Here, we show that SHY2, an auxin/indole-3-acetic acid (Aux/IAA) repressor, exhibits a dose-dependent bimodal role in auxin-sensitive root-hair growth and gene transcription in Arabidopsis, whereas other Aux/IAA homologs consistently repress the auxin responses. The co-repressor (TOPLESS [TPL])-binding affinity of a bimodal Aux/IAA was lower than that of a consistently repressing Aux/IAA. The switch of a single amino acid residue in the TPL-binding motif between the bimodal form and the consistently repressing form switched their TPL-binding affinity and transcriptional and biological roles in auxin responses. Based on these data, we propose a model whereby competition between homologous repressors with different co-repressor-binding affinities could generate a bimodal output at the transcriptional and developmental levels.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1407-1422"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proxitome profiling reveals a conserved SGT1-NSL1 signaling module that activates NLR-mediated immunity. Proxitome 图谱揭示了激活 NLR 介导的免疫的保守 SGT1-NSL1 信号模块。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-25 DOI: 10.1016/j.molp.2024.07.010

Dingliang Zhang, Xinxin Yang, Zhiyan Wen, Zhen Li, Xinyu Zhang, Chenchen Zhong, Jiajie She, Qianshen Zhang, He Zhang, Wenli Li, Xiaoyun Zhao, Mingliang Xu, Zhen Su, Dawei Li, Savithramma P Dinesh-Kumar, Yongliang Zhang

{"title":"Proxitome profiling reveals a conserved SGT1-NSL1 signaling module that activates NLR-mediated immunity.","authors":"Dingliang Zhang, Xinxin Yang, Zhiyan Wen, Zhen Li, Xinyu Zhang, Chenchen Zhong, Jiajie She, Qianshen Zhang, He Zhang, Wenli Li, Xiaoyun Zhao, Mingliang Xu, Zhen Su, Dawei Li, Savithramma P Dinesh-Kumar, Yongliang Zhang","doi":"10.1016/j.molp.2024.07.010","DOIUrl":"10.1016/j.molp.2024.07.010","url":null,"abstract":"Suppressor of G2 allele of skp1 (SGT1) is a highly conserved eukaryotic protein that plays a vital role in growth, development, and immunity in both animals and plants. Although some SGT1 interactors have been identified, the molecular regulatory network of SGT1 remains unclear. SGT1 serves as a co-chaperone to stabilize protein complexes such as the nucleotide-binding leucine-rich repeat (NLR) class of immune receptors, thereby positively regulating plant immunity. SGT1 has also been found to be associated with the SKP1-Cullin-F-box (SCF) E3 ubiquitin ligase complex. However, whether SGT1 targets immune repressors to coordinate plant immune activation remains elusive. In this study, we constructed a toolbox for TurboID- and split-TurboID-based proximity labeling (PL) assays in Nicotiana benthamiana and used the PL toolbox to explore the SGT1 interactome during pre- and post-immune activation. The comprehensive SGT1 interactome network we identified highlights a dynamic shift from proteins associated with plant development to those linked with plant immune responses. We found that SGT1 interacts with Necrotic Spotted Lesion 1 (NSL1), which negatively regulates salicylic acid-mediated defense by interfering with the nucleocytoplasmic trafficking of non-expressor of pathogenesis-related genes 1 (NPR1) during N NLR-mediated response to tobacco mosaic virus. SGT1 promotes the SCF-dependent degradation of NSL1 to facilitate immune activation, while salicylate-induced protein kinase-mediated phosphorylation of SGT1 further potentiates this process. Besides N NLR, NSL1 also functions in several other NLR-mediated immunity. Collectively, our study unveils the regulatory landscape of SGT1 and reveals a novel SGT1-NSL1 signaling module that orchestrates plant innate immunity.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1369-1391"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SIZ1-mediated SUMOylation of CPSF100 promotes plant thermomorphogenesis by controlling alternative polyadenylation. SIZ1介导的CPSF100的SUMOlation通过控制替代多聚腺苷酸化促进植物的热形态发生。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-09-02 Epub Date: 2024-07-25 DOI: 10.1016/j.molp.2024.07.011

Zhibo Yu, Jun Wang, Cheng Zhang, Qiuna Zhan, Leqian Shi, Bing Song, Danlu Han, Jieming Jiang, Junwen Huang, Xiaolin Ou, Zhonghui Zhang, Jianbin Lai, Qingshun Quinn Li, Chengwei Yang

{"title":"SIZ1-mediated SUMOylation of CPSF100 promotes plant thermomorphogenesis by controlling alternative polyadenylation.","authors":"Zhibo Yu, Jun Wang, Cheng Zhang, Qiuna Zhan, Leqian Shi, Bing Song, Danlu Han, Jieming Jiang, Junwen Huang, Xiaolin Ou, Zhonghui Zhang, Jianbin Lai, Qingshun Quinn Li, Chengwei Yang","doi":"10.1016/j.molp.2024.07.011","DOIUrl":"10.1016/j.molp.2024.07.011","url":null,"abstract":"Under warm temperatures, plants adjust their morphologies for environmental adaption via precise gene expression regulation. However, the function and regulation of alternative polyadenylation (APA), an important fine-tuning of gene expression, remains unknown in plant thermomorphogenesis. In this study, we found that SUMOylation, a critical post-translational modification, is induced by a long-term treatment at warm temperatures via a SUMO ligase SIZ1 in Arabidopsis. Disruption of SIZ1 altered the global usage of polyadenylation signals and affected the APA dynamic of thermomorphogenesis-related genes. CPSF100, a key subunit of the CPSF complex for polyadenylation regulation, is SUMOylated by SIZ1. Importantly, we demonstrated that SUMOylation is essential for the function of CPSF100 in genome-wide polyadenylation site choice during thermomorphogenesis. Further analyses revealed that the SUMO conjugation on CPSF100 attenuates its interaction with two isoforms of its partner CPSF30, increasing the nuclear accumulation of CPSF100 for polyadenylation regulation. In summary, our study uncovers a regulatory mechanism of APA via SIZ1-mediated SUMOylation in plant thermomorphogenesis.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1392-1406"},"PeriodicalIF":17.1,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple. ACC 氧化酶 3 mRNA 从种子到果肉的移动促进了苹果果实的成熟。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-19 DOI: 10.1016/j.molp.2024.06.008

Ting Wang, Yi Zheng, Chen Xu, Yulin Deng, Xinyi Hao, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu

{"title":"Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple.","authors":"Ting Wang, Yi Zheng, Chen Xu, Yulin Deng, Xinyi Hao, Zicheng Chu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu","doi":"10.1016/j.molp.2024.06.008","DOIUrl":"10.1016/j.molp.2024.06.008","url":null,"abstract":"Xenia, the phenomenon in which the pollen genotype directly affects the phenotypic characteristics of maternal tissues (i.e., fruit ripening), has applications in crop production and breeding. However, the underlying molecular mechanism has yet to be elucidated. Here, we investigated whether mobile mRNAs from the pollen affect the ripening and quality-related characteristics of the fruit using cross-pollination between distinct Malus domestica (apple) cultivars. We demonstrated that hundreds of mobile mRNAs originating from the seeds are delivered to the fruit. We found that the movement of one of these mRNAs, ACC oxidase 3 (MdACO3), is coordinated with fruit ripening. Salicylic acid treatment, which can cause plasmodesmal closure, blocks MdACO3 movement, indicating that MdACO3 transcripts may move through the plasmodesmata. To assess the role of mobile MdACO3 transcripts in apple fruit, we created MdACO3-GFP-expressing apple seeds using MdACO3-GFP-overexpressing pollen for pollination and showed that MdACO3 transcripts in the transgenic seeds move to the flesh, where they promote fruit ripening. Furthermore, we demonstrated that MdACO3 can be transported from the seeds to fruit in the fleshy-fruited species tomato and strawberry. These results underscore the potential of mobile mRNAs from seeds to influence fruit characteristics, providing an explanation for the xenia phenomenon. Notably, our findings highlight the feasibility of leveraging diverse pollen genomic resources, without resorting to genome editing, to improve fruit quality.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1221-1235"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141432352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging plant biomechanics in multiscale plant systems for sustainable innovations. 在多尺度植物系统中利用植物生物力学进行可持续创新。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-05 DOI: 10.1016/j.molp.2024.07.002

Jinbo Shen, Yansong Miao

引用次数: 0

Development and maintenance of the ligular region of maize leaves. 玉米叶片韧带区的发育和维持。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-11 DOI: 10.1016/j.molp.2024.07.004

Josh Strable, Alejandro Aragón-Raygoza

引用次数: 0

CONSTANS alters the circadian clock in Arabidopsis thaliana. CONSTANS 改变了拟南芥的昼夜节律。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-17 DOI: 10.1016/j.molp.2024.06.006

Pedro de Los Reyes, Gloria Serrano-Bueno, Francisco J Romero-Campero, He Gao, Jose M Romero, Federico Valverde

{"title":"CONSTANS alters the circadian clock in Arabidopsis thaliana.","authors":"Pedro de Los Reyes, Gloria Serrano-Bueno, Francisco J Romero-Campero, He Gao, Jose M Romero, Federico Valverde","doi":"10.1016/j.molp.2024.06.006","DOIUrl":"10.1016/j.molp.2024.06.006","url":null,"abstract":"Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment. Among these processes, the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks. The photoperiodic pathway, which controls plant response to day length, is one of the most important pathways controlling flowering. In Arabidopsis photoperiodic flowering, CONSTANS (CO) is the central gene activating the expression of the florigen FLOWERING LOCUS T (FT) in the leaves at the end of a long day. The circadian clock strongly regulates CO expression. However, to date, no evidence has been reported regarding a feedback loop from the photoperiod pathway back to the circadian clock. Using transcriptional networks, we have identified relevant network motifs regulating the interplay between the circadian clock and the photoperiod pathway. Gene expression, chromatin immunoprecipitation experiments, and phenotypic analysis allowed us to elucidate the role of CO over the circadian clock. Plants with altered CO expression showed a different internal clock period, measured by daily leaf rhythmic movements. We showed that CO upregulates the expression of key genes related to the circadian clock, such as CCA1, LHY, PRR5, and GI, at the end of a long day by binding to specific sites on their promoters. Moreover, a high number of PRR5-repressed target genes are upregulated by CO, and this could explain the phase transition promoted by CO. The CO-PRR5 complex interacts with the bZIP transcription factor HY5 and helps to localize the complex in the promoters of clock genes. Taken together, our results indicate that there may be a feedback loop in which CO communicates back to the circadian clock, providing seasonal information to the circadian system.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1204-1220"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141419953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixing and matching SMXL proteins to fine-tune strigolactone responses. 混合和匹配 SMXL 蛋白以微调绞股蓝内酯反应。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-18 DOI: 10.1016/j.molp.2024.06.009

Jenna E Hountalas, Shelley Lumba

引用次数: 0