Molecular Plant最新文献_第7页

The metal tolerance protein OsMTP11 facilitates cadmium sequestration in the vacuoles of leaf vascular cells for restricting its translocation into rice grains. 金属耐受蛋白 OsMTP11 有助于将镉封存在叶片维管细胞的液泡中，从而限制镉向水稻籽粒的转移。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-01 DOI: 10.1016/j.molp.2024.09.012

Peng Liu, Liang Sun, Yu Zhang, Yongjun Tan, Yuxing Zhu, Can Peng, Jiurong Wang, Huili Yan, Donghai Mao, Guohua Liang, Gang Liang, Xiaoxiang Li, Yuntao Liang, Feng Wang, Zhenyan He, Wenbang Tang, Daoyou Huang, Caiyan Chen

{"title":"The metal tolerance protein OsMTP11 facilitates cadmium sequestration in the vacuoles of leaf vascular cells for restricting its translocation into rice grains.","authors":"Peng Liu, Liang Sun, Yu Zhang, Yongjun Tan, Yuxing Zhu, Can Peng, Jiurong Wang, Huili Yan, Donghai Mao, Guohua Liang, Gang Liang, Xiaoxiang Li, Yuntao Liang, Feng Wang, Zhenyan He, Wenbang Tang, Daoyou Huang, Caiyan Chen","doi":"10.1016/j.molp.2024.09.012","DOIUrl":"10.1016/j.molp.2024.09.012","url":null,"abstract":"Rice (Oryza sativa) provides >20% of the consumed calories in the human diet. However, rice is also a leading source of dietary cadmium (Cd) that seriously threatens human health. Deciphering the genetic network that underlies the grain-Cd accumulation will benefit the development of low-Cd rice and mitigate the effects of Cd accumulation in the rice grain. In this study, we identified a QTL gene, OsCS1, which is allelic to OsMTP11 and encodes a protein sequestering Cd in the leaf during vegetative growth and preventing Cd from being translocated to the grain after heading in rice. OsCS1 is predominantly expressed in leaf vascular parenchyma cells, where it binds to a vacuole-sorting receptor protein OsVSR2 and is translocated intracellularly from the trans-Golgi network to pre-vacuolar compartments and then to the vacuole. In this trafficking process, OsCS1 actively transports Cd into the endomembrane system and sequesters it in the vacuoles. There are natural variations in the promoter of OsCS1 between the indica and japonica rice subspecies. Duplication of a G-box-like motif in the promoter region of the superior allele of OsCS1 from indica rice enhances the binding of the transcription factor OsIRO2 to the OsCS1 promoter, thereby promoting OsCS1 expression. Introgression of this allele into commercial rice varieties could significantly lower grain-Cd levels compared to the inferior allele present in japonica rice. Collectively, our findings offer new insights into the genetic control of leaf-to-grain Cd translocation and provide a novel gene and its superior allele for the genetic improvement of low-Cd variety in rice.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1733-1752"},"PeriodicalIF":17.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanopore ultra-long sequencing and adaptive sampling spur plant complete telomere-to-telomere genome assembly. 纳米孔超长测序和自适应取样技术促进了植物端粒到端粒基因组的完全组装。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-16 DOI: 10.1016/j.molp.2024.10.008

Dongdong Lu, Caijuan Liu, Wenjun Ji, Ruiyan Xia, Shanshan Li, Yanxia Liu, Naixu Liu, Yongqi Liu, Xing Wang Deng, Bosheng Li

引用次数: 0

The cartography of plant immunity: Proximity labeling puts a novel SGT1-NSL1 regulatory module on the map. 植物免疫制图：近距离标记将新型 SGT1-NSL1 调控模块置于地图上。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-04 DOI: 10.1016/j.molp.2024.10.003

Huang Tan, Chaonan Shi, Alberto P Macho, Rosa Lozano-Durán

引用次数: 0

Next-generation research on transcriptional regulation of plant immunity. 植物免疫转录调控的新一代研究。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-11 DOI: 10.1016/j.molp.2024.10.005

Akira Mine

引用次数: 0

Increasing seed lint fiber density for promoting cotton yield: Opportunities and challenges. 提高籽棉纤维密度以提高棉花产量：机遇与挑战。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-09-02 DOI: 10.1016/j.molp.2024.08.010

Tianlun Zhao, Jinhong Chen, Shuijin Zhu, Qian-Hao Zhu

引用次数: 0

Creation of folate-biofortified rice by simultaneously enhancing biosynthetic flux and blocking folate oxidation. 通过同时提高生物合成通量和阻止叶酸氧化，生产叶酸生物强化水稻。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-09-16 DOI: 10.1016/j.molp.2024.09.005

Qiuju Liang, Wei Zhang, Jianzhou Pang, Shuncong Zhang, Xiaowan Hou, Chunyi Zhang

引用次数: 0

Efficient control of root-knot nematodes by expressing Bt nematicidal proteins in root leucoplasts. 通过在根白质中表达 Bt 杀线虫蛋白有效控制根结线虫。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-08-14 DOI: 10.1016/j.molp.2024.08.004

Yong Wang, Mengnan Wang, Yali Zhang, Longwei Peng, Dadong Dai, Fengjuan Zhang, Jiang Zhang

{"title":"Efficient control of root-knot nematodes by expressing Bt nematicidal proteins in root leucoplasts.","authors":"Yong Wang, Mengnan Wang, Yali Zhang, Longwei Peng, Dadong Dai, Fengjuan Zhang, Jiang Zhang","doi":"10.1016/j.molp.2024.08.004","DOIUrl":"10.1016/j.molp.2024.08.004","url":null,"abstract":"Root-knot nematodes (RKNs) are plant pests that infect the roots of host plants. Bacillus thuringiensis (Bt) nematicidal proteins exhibited toxicity to nematodes. However, the application of nematicidal proteins for plant protection is hampered by the lack of effective delivery systems in transgenic plants. In this study, we discovered the accumulation of leucoplasts (root plastids) in galls and RKN-induced giant cells. RKN infection causes the degradation of leucoplasts into small vesicle-like structures, which are responsible for delivering proteins to RKNs, as observed through confocal microscopy and immunoelectron microscopy. We showed that different-sized proteins from leucoplasts could be taken up by Meloidogyne incognita female. To further explore the potential applications of leucoplasts, we introduced the Bt crystal protein Cry5Ba2 into tobacco and tomato leucoplasts by fusing it with a transit peptide. The transgenic plants showed significant resistance to RKNs. Intriguingly, RKN females preferentially took up Cry5Ba2 protein when delivered through plastids rather than the cytosol. The decrease in progeny was positively correlated with the delivery efficiency of the nematicidal protein. In conclusion, this study offers new insights into the feeding behavior of RKNs and their ability to ingest leucoplast proteins, and demonstrates that root leucoplasts can be used for delivering nematicidal proteins, thereby offering a promising approach for nematode control.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1504-1519"},"PeriodicalIF":17.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The OsSRO1c-OsDREB2B complex undergoes protein phase transition to enhance cold tolerance in rice. OsSRO1c-OsDREB2B 复合物经历蛋白质相变以增强水稻的耐寒性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-08-22 DOI: 10.1016/j.molp.2024.08.006

Dan Hu, Yilong Yao, Yan Lv, Jun You, Yu Zhang, Qingya Lv, Jiawei Li, Stephanie Hutin, Haiyan Xiong, Chloe Zubieta, Xuelei Lai, Lizhong Xiong

{"title":"The OsSRO1c-OsDREB2B complex undergoes protein phase transition to enhance cold tolerance in rice.","authors":"Dan Hu, Yilong Yao, Yan Lv, Jun You, Yu Zhang, Qingya Lv, Jiawei Li, Stephanie Hutin, Haiyan Xiong, Chloe Zubieta, Xuelei Lai, Lizhong Xiong","doi":"10.1016/j.molp.2024.08.006","DOIUrl":"10.1016/j.molp.2024.08.006","url":null,"abstract":"Cold stress is one of the major abiotic stress factors affecting rice growth and development, leading to significant yield loss in the context of global climate change. Exploring natural variants that confer cold resistance and the underlying molecular mechanism responsible for this is the major strategy to breed cold-tolerant rice varieties. Here, we show that natural variations of a SIMILAR to RCD ONE (SRO) gene, OsSRO1c, confer cold tolerance in rice at both seedling and booting stages. Our in vivo and in vitro experiments demonstrated that OsSRO1c possesses intrinsic liquid-liquid phase-separation ability and recruits OsDREB2B, an AP2/ERF transcription factor that functions as a positive regulator of cold stress, into its biomolecular condensates in the nucleus, resulting in elevated transcriptional activity of OsDREB2B. We found that the OsSRO1c-OsDREB2B complex directly responds to low temperature through dynamic phase transitions and regulates key cold-response genes, including COLD1. Furthermore, we showed that introgression of an elite haplotype of OsSRO1c into a cold-susceptible indica rice could significantly increase its cold resistance. Collectively, our work reveals a novel cold-tolerance regulatory module in rice and provides promising genetic targets for molecular breeding of cold-tolerant rice varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1520-1538"},"PeriodicalIF":17.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strigolactone-gibberellin crosstalk mediated by a distant silencer fine-tunes plant height in upland cotton. 由遥远的沉默因子介导的赤霉内酯-赤霉素串扰微调了陆地棉的植株高度。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-08-22 DOI: 10.1016/j.molp.2024.08.007

Zailong Tian, Baojun Chen, Hongge Li, Xinxin Pei, Yaru Sun, Gaofei Sun, Zhaoe Pan, Panhong Dai, Xu Gao, Xiaoli Geng, Zhen Peng, Yinhua Jia, Daowu Hu, Liru Wang, Baoyin Pang, Ai Zhang, Xiongming Du, Shoupu He

{"title":"Strigolactone-gibberellin crosstalk mediated by a distant silencer fine-tunes plant height in upland cotton.","authors":"Zailong Tian, Baojun Chen, Hongge Li, Xinxin Pei, Yaru Sun, Gaofei Sun, Zhaoe Pan, Panhong Dai, Xu Gao, Xiaoli Geng, Zhen Peng, Yinhua Jia, Daowu Hu, Liru Wang, Baoyin Pang, Ai Zhang, Xiongming Du, Shoupu He","doi":"10.1016/j.molp.2024.08.007","DOIUrl":"10.1016/j.molp.2024.08.007","url":null,"abstract":"Optimal plant height is crucial in modern agriculture, influencing lodging resistance and facilitating mechanized crop production. Upland cotton (Gossypium hirsutum) is the most important fiber crop globally; however, the genetic basis underlying plant height remains largely unexplored. In this study, we conducted a genome-wide association study to identify a major locus controlling plant height (PH1) in upland cotton. This locus encodes gibberellin 2-oxidase 1A (GhPH1) and features a 1133-bp structural variation (PAVPH1) located approximately 16 kb upstream. The presence or absence of PAVPH1 influences the expression of GhPH1, thereby affecting plant height. Further analysis revealed that a gibberellin-regulating transcription factor (GhGARF) recognizes and binds to a specific CATTTG motif in both the GhPH1 promoter and PAVPH1. This interaction downregulates GhPH1, indicating that PAVPH1 functions as a distant upstream silencer. Intriguingly, we found that DWARF53 (D53), a key repressor of the strigolactone (SL) signaling pathway, directly interacts with GhGARF to inhibit its binding to targets. Moreover, we identified a previously unrecognized gibberellin-SL crosstalk mechanism mediated by the GhD53-GhGARF-GhPH1/PAVPH1 module, which is crucial for regulating plant height in upland cotton. These findings shed light on the genetic basis and gene interaction network underlying plant height, providing valuable insights for the development of semi-dwarf cotton varieties through precise modulation of GhPH1 expression.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1539-1557"},"PeriodicalIF":17.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The OsNAC41-RoLe1-OsAGAP module promotes root development and drought resistance in upland rice. OsNAC41-RoLe1-OsAGAP 模块促进高原水稻根系发育和耐旱性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-10-07 Epub Date: 2024-09-02 DOI: 10.1016/j.molp.2024.09.002

Shichen Han, Yulong Wang, Yingxiu Li, Rui Zhu, Yunsong Gu, Jin Li, Haifeng Guo, Wei Ye, Hafiz Ghualm Nabi, Tao Yang, Yanming Wang, Pengli Liu, Junzhi Duan, Xingming Sun, Zhanying Zhang, Hongliang Zhang, Zichao Li, Jinjie Li

{"title":"The OsNAC41-RoLe1-OsAGAP module promotes root development and drought resistance in upland rice.","authors":"Shichen Han, Yulong Wang, Yingxiu Li, Rui Zhu, Yunsong Gu, Jin Li, Haifeng Guo, Wei Ye, Hafiz Ghualm Nabi, Tao Yang, Yanming Wang, Pengli Liu, Junzhi Duan, Xingming Sun, Zhanying Zhang, Hongliang Zhang, Zichao Li, Jinjie Li","doi":"10.1016/j.molp.2024.09.002","DOIUrl":"10.1016/j.molp.2024.09.002","url":null,"abstract":"Drought is a major environmental stress limiting crop yields worldwide. Upland rice (Oryza sativa) has evolved complex genetic mechanisms for adaptative growth under drought stress. However, few genetic variants that mediate drought resistance in upland rice have been identified, and little is known about the evolution of this trait during rice domestication. In this study, using a genome-wide association study we identified ROOT LENGTH 1 (RoLe1) that controls rice root length and drought resistance. We found that a G-to-T polymorphism in the RoLe1 promoter causes increased binding of the transcription factor OsNAC41 and thereby enhanced expression of RoLe1. We further showed that RoLe1 interacts with OsAGAP, an ARF-GTPase activating protein involved in auxin-dependent root development, and interferes with its function to modulate root development. Interestingly, RoLe1 could enhance crop yield by increasing the seed-setting rate under moderate drought conditions. Genomic evolutionary analysis revealed that a newly arisen favorable allelic variant, proRoLe1-526T, originated from the midwest Asia and was retained in upland rice during domestication. Collectively, our study identifies an OsNAC41-RoLe1-OsAGAP module that promotes upland rice root development and drought resistance, providing promising genetic targets for molecular breeding of drought-resistant rice varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1573-1593"},"PeriodicalIF":17.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0