Molecular Plant最新文献_第7页

Mixing and matching SMXL proteins to fine-tune strigolactone responses. 混合和匹配 SMXL 蛋白以微调绞股蓝内酯反应。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-18 DOI: 10.1016/j.molp.2024.06.009

Jenna E Hountalas, Shelley Lumba

引用次数: 0

A near-complete cucumber reference genome assembly and Cucumber-DB, a multi-omics database. 近乎完整的黄瓜参考基因组组装和多组学数据库 Cucumber-DB。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-20 DOI: 10.1016/j.molp.2024.06.012

Jiantao Guan, Han Miao, Zhonghua Zhang, Shaoyun Dong, Qi Zhou, Xiaoping Liu, Diane M Beckles, Xingfang Gu, Sanwen Huang, Shengping Zhang

引用次数: 0

Phenotyping floral attractiveness to pollinators using volatilomics, 3D imaging, and insect monitoring. 利用挥发物组学、三维成像和昆虫监测对花朵对传粉昆虫的吸引力进行表型分析。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-20 DOI: 10.1016/j.molp.2024.06.011

Filip Slavković, Adnane Boualem, Catherine Dogimont, Abdelhafid Bendahmane

引用次数: 0

Steroidal scaffold decorations in Solanum alkaloid biosynthesis. 茄科生物碱生物合成中的类固醇支架装饰。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-26 DOI: 10.1016/j.molp.2024.06.013

Rosalind Lucier, Mohamed O Kamileen, Yoko Nakamura, Sofiia Serediuk, Ranjit Barbole, Jens Wurlitzer, Maritta Kunert, Sarah Heinicke, Sarah E O'Connor, Prashant D Sonawane

{"title":"Steroidal scaffold decorations in Solanum alkaloid biosynthesis.","authors":"Rosalind Lucier, Mohamed O Kamileen, Yoko Nakamura, Sofiia Serediuk, Ranjit Barbole, Jens Wurlitzer, Maritta Kunert, Sarah Heinicke, Sarah E O'Connor, Prashant D Sonawane","doi":"10.1016/j.molp.2024.06.013","DOIUrl":"10.1016/j.molp.2024.06.013","url":null,"abstract":"Steroidal glycoalkaloids (SGAs) are specialized metabolites produced by hundreds of Solanum species, including important vegetable crops such as tomato, potato, and eggplant. Although it has been known that SGAs play important roles in defense in plants and \"anti-nutritional\" effects (e.g., toxicity and bitterness) to humans, many of these molecules have documented anti-cancer, anti-microbial, anti-inflammatory, anti-viral, and anti-pyretic activities. Among these, α-solasonine and α-solamargine isolated from black nightshade (Solanum nigrum) are reported to have potent anti-tumor, anti-proliferative, and anti-inflammatory activities. Notably, α-solasonine and α-solamargine, along with the core steroidal aglycone solasodine, are the most widespread SGAs produced among the Solanum plants. However, it is still unknown how plants synthesize these bioactive steroidal molecules. Through comparative metabolomic-transcriptome-guided approach, biosynthetic logic, combinatorial expression in Nicotiana benthamiana, and functional recombinant enzyme assays, here we report the discovery of 12 enzymes from S. nigrum that converts the starting cholesterol precursor to solasodine aglycone, and the downstream α-solasonine, α-solamargine, and malonyl-solamargine SGA products. We further identified six enzymes from cultivated eggplant that catalyze the production of α-solasonine, α-solamargine, and malonyl-solamargine SGAs from solasodine aglycone via glycosylation and atypical malonylation decorations. Our work provides the gene tool box and platform for engineering the production of high-value, steroidal bioactive molecules in heterologous hosts using synthetic biology.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1236-1254"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic insights into adaptation of alfalfa. 对紫花苜蓿适应性的遗传学见解。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-28 DOI: 10.1016/j.molp.2024.06.015

Yafei Guo, Lipeng Kang, Fei Lu

引用次数: 0

Phytochrome B interacts with LIGULELESS1 to control plant architecture and density tolerance in maize. 植物色素 B 与 LIGULELESS1 相互作用，控制玉米的植株结构和耐密度。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-29 DOI: 10.1016/j.molp.2024.06.014

Qingbiao Shi, Ying Xia, Qibin Wang, Kaiwen Lv, Hengjia Yang, Lianzhe Cui, Yue Sun, Xiaofei Wang, Qing Tao, Xiehai Song, Di Xu, Wenchang Xu, Xingyun Wang, Xianglan Wang, Fanying Kong, Haisen Zhang, Bosheng Li, Pinghua Li, Haiyang Wang, Gang Li

{"title":"Phytochrome B interacts with LIGULELESS1 to control plant architecture and density tolerance in maize.","authors":"Qingbiao Shi, Ying Xia, Qibin Wang, Kaiwen Lv, Hengjia Yang, Lianzhe Cui, Yue Sun, Xiaofei Wang, Qing Tao, Xiehai Song, Di Xu, Wenchang Xu, Xingyun Wang, Xianglan Wang, Fanying Kong, Haisen Zhang, Bosheng Li, Pinghua Li, Haiyang Wang, Gang Li","doi":"10.1016/j.molp.2024.06.014","DOIUrl":"10.1016/j.molp.2024.06.014","url":null,"abstract":"Over the past few decades, significant improvements in maize yield have been largely attributed to increased plant density of upright hybrid varieties rather than increased yield per plant. However, dense planting triggers shade avoidance responses (SARs) that optimize light absorption but impair plant vigor and performance, limiting yield improvement through increasing plant density. In this study, we demonstrated that high-density-induced leaf angle narrowing and stem/stalk elongation are largely dependent on phytochrome B (phyB1/B2), the primary photoreceptor responsible for perceiving red (R) and far-red (FR) light in maize. We found that maize phyB physically interacts with the LIGULELESS1 (LG1), a classical key regulator of leaf angle, to coordinately regulate plant architecture and density tolerance. The abundance of LG1 is significantly increased by phyB under high R:FR light (low density) but rapidly decreases under low R:FR light (high density), correlating with variations in leaf angle and plant height under various densities. In addition, we identified the homeobox transcription factor HB53 as a target co-repressed by both phyB and LG1 but rapidly induced by canopy shade. Genetic and cellular analyses showed that HB53 regulates plant architecture by controlling the elongation and division of ligular adaxial and abaxial cells. Taken together, these findings uncover the phyB-LG1-HB53 regulatory module as a key molecular mechanism governing plant architecture and density tolerance, providing potential genetic targets for breeding maize hybrid varieties suitable for high-density planting.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1255-1271"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SnRK1a1, a new player in the sucrose-Opaque2 network during endosperm filling. SnRK1a1是胚乳填充过程中蔗糖-Opaque2网络中的一个新角色。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-06 DOI: 10.1016/j.molp.2024.07.003

Kelly Mayrink Balmant, Marcio F R Resende

引用次数: 0

Structural variations of a new fertility restorer gene, Rf20, underlie the restoration of wild abortive-type cytoplasmic male sterility in rice. 一种新的生育力恢复基因 Rf20 的结构变异是恢复水稻野生败育型细胞质雄性不育的基础。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-02 DOI: 10.1016/j.molp.2024.07.001

Shufeng Song, Yixing Li, Mudan Qiu, Na Xu, Bin Li, Longhui Zhang, Lei Li, Weijun Chen, Jinglei Li, Tiankang Wang, Yingxin Qiu, Mengmeng Gong, Dong Yu, Hao Dong, Siqi Xia, Yi Pan, Dingyang Yuan, Li Li

{"title":"Structural variations of a new fertility restorer gene, Rf20, underlie the restoration of wild abortive-type cytoplasmic male sterility in rice.","authors":"Shufeng Song, Yixing Li, Mudan Qiu, Na Xu, Bin Li, Longhui Zhang, Lei Li, Weijun Chen, Jinglei Li, Tiankang Wang, Yingxin Qiu, Mengmeng Gong, Dong Yu, Hao Dong, Siqi Xia, Yi Pan, Dingyang Yuan, Li Li","doi":"10.1016/j.molp.2024.07.001","DOIUrl":"10.1016/j.molp.2024.07.001","url":null,"abstract":"The discovery of a wild abortive-type (WA) cytoplasmic male sterile (CMS) line and breeding its restorer line have led to the commercialization of three-line hybrid rice, contributing considerably to global food security. However, the molecular mechanisms underlying fertility abortion and the restoration of CMS-WA lines remain largely elusive. In this study, we cloned a restorer gene, Rf20, following a genome-wide association study analysis of the core parent lines of three-line hybrid rice. We found that Rf20 was present in all core parental lines, but different haplotypes and structural variants of its gene resulted in differences in Rf20 expression levels between sterile and restored lines. Rf20 could restore pollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMS lines under high temperatures. In addition, we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11. This interaction enhances COX11's function as a scavenger of reactive oxygen species, which in turn restores pollen fertility. Collectively, our study suggests a new action mode for pentatricopeptide repeat proteins in the fertility restoration of CMS lines, providing an essential theoretical basis for breeding robust restorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1272-1288"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The jasmonate pathway promotes nodule symbiosis and suppresses host plant defense in Medicago truncatula. 茉莉酸盐通路促进麦地那龙葵（Medicago truncatula）的结核共生并抑制宿主植物的防御。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-06-09 DOI: 10.1016/j.molp.2024.06.004

Da Guo, Jingrui Li, Peng Liu, Yuzhan Wang, Na Cao, Xiangling Fang, Tao Wang, Jiangli Dong

{"title":"The jasmonate pathway promotes nodule symbiosis and suppresses host plant defense in Medicago truncatula.","authors":"Da Guo, Jingrui Li, Peng Liu, Yuzhan Wang, Na Cao, Xiangling Fang, Tao Wang, Jiangli Dong","doi":"10.1016/j.molp.2024.06.004","DOIUrl":"10.1016/j.molp.2024.06.004","url":null,"abstract":"Root nodule symbiosis (RNS) between legumes and rhizobia is a major source of nitrogen in agricultural systems. Effective symbiosis requires precise regulation of plant defense responses. The role of the defense hormone jasmonic acid (JA) in the immune response has been extensively studied. Current research shows that JA can play either a positive or negative regulatory role in RNS depending on its concentration, but the molecular mechanisms remain to be elucidated. In this study, we found that inoculation with the rhizobia Sm1021 induces the JA pathway in Medicago truncatula, and blocking the JA pathway significantly reduces the number of infection threads. Mutations in the MtMYC2 gene, which encodes a JA signaling master transcription factor, significantly inhibited rhizobia infection, terminal differentiation, and symbiotic cell formation. Combining RNA sequencing and chromatin immunoprecipitation sequencing, we discovered that MtMYC2 regulates the expression of nodule-specific MtDNF2, MtNAD1, and MtSymCRK to suppress host defense, while it activates MtDNF1 expression to regulate the maturation of MtNCRs, which in turn promotes bacteroid formation. More importantly, MtMYC2 participates in symbiotic signal transduction by promoting the expression of MtIPD3. Notably, the MtMYC2-MtIPD3 transcriptional regulatory module is specifically present in legumes, and the Mtmyc2 mutants are susceptible to the infection by the pathogen Rhizoctonia solani. Collectively, these findings reveal the molecular mechanisms of how the JA pathway regulates RNS, broadening our understanding of the roles of JA in plant-microbe interactions.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1183-1203"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hordedane diterpenoid phytoalexins restrict Fusarium graminearum infection but enhance Bipolaris sorokiniana colonization of barley roots. Hordedane 二萜类植物毒素限制了禾谷镰刀菌的感染，但增强了大麦根部 Bipolaris sorkiniana 的定殖。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-08-05 Epub Date: 2024-07-30 DOI: 10.1016/j.molp.2024.07.006

Yaming Liu, Dario Esposto, Lisa K Mahdi, Andrea Porzel, Pauline Stark, Hidayat Hussain, Anja Scherr-Henning, Simon Isfort, Ulschan Bathe, Iván F Acosta, Alga Zuccaro, Gerd U Balcke, Alain Tissier

{"title":"Hordedane diterpenoid phytoalexins restrict Fusarium graminearum infection but enhance Bipolaris sorokiniana colonization of barley roots.","authors":"Yaming Liu, Dario Esposto, Lisa K Mahdi, Andrea Porzel, Pauline Stark, Hidayat Hussain, Anja Scherr-Henning, Simon Isfort, Ulschan Bathe, Iván F Acosta, Alga Zuccaro, Gerd U Balcke, Alain Tissier","doi":"10.1016/j.molp.2024.07.006","DOIUrl":"10.1016/j.molp.2024.07.006","url":null,"abstract":"Plant immunity is a multilayered process that includes recognition of patterns or effectors from pathogens to elicit defense responses. These include the induction of a cocktail of defense metabolites that typically restrict pathogen virulence. Here, we investigate the interaction between barley roots and the fungal pathogens Bipolaris sorokiniana (Bs) and Fusarium graminearum (Fg) at the metabolite level. We identify hordedanes, a previously undescribed set of labdane-related diterpenoids with antimicrobial properties, as critical players in these interactions. Infection of barley roots by Bs and Fg elicits hordedane synthesis from a 600-kb gene cluster. Heterologous reconstruction of the biosynthesis pathway in yeast and Nicotiana benthamiana produced several hordedanes, including one of the most functionally decorated products 19-β-hydroxy-hordetrienoic acid (19-OH-HTA). Barley mutants in the diterpene synthase genes of this cluster are unable to produce hordedanes but, unexpectedly, show reduced Bs colonization. By contrast, colonization by Fusarium graminearum, another fungal pathogen of barley and wheat, is 4-fold higher in the mutants completely lacking hordedanes. Accordingly, 19-OH-HTA enhances both germination and growth of Bs, whereas it inhibits other pathogenic fungi, including Fg. Analysis of microscopy and transcriptomics data suggest that hordedanes delay the necrotrophic phase of Bs. Taken together, these results show that adapted pathogens such as Bs can subvert plant metabolic defenses to facilitate root colonization.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1307-1327"},"PeriodicalIF":17.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141600789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0