Molecular Plant最新文献_第7页

Heat stress-induced decapping of WUSCHEL mRNA enhances stem cell thermotolerance in Arabidopsis. 热胁迫诱导的 WUSCHEL mRNA 脱帽可增强拟南芥干细胞的耐热性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-10-28 DOI: 10.1016/j.molp.2024.10.011

Sumei Liu, Haijun Wu, Zhong Zhao

引用次数: 0

Volatilome-based GWAS identifies OsWRKY19 and OsNAC021 as key regulators of rice aroma. 基于挥发物的 GWAS 发现 OsWRKY19 和 OsNAC021 是水稻香气的关键调控因子。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-12 DOI: 10.1016/j.molp.2024.11.002

Yan Li, Yuanyuan Miao, Honglun Yuan, Fengkun Huang, Mingqi Sun, Liqiang He, Xianqing Liu, Jie Luo

{"title":"Volatilome-based GWAS identifies OsWRKY19 and OsNAC021 as key regulators of rice aroma.","authors":"Yan Li, Yuanyuan Miao, Honglun Yuan, Fengkun Huang, Mingqi Sun, Liqiang He, Xianqing Liu, Jie Luo","doi":"10.1016/j.molp.2024.11.002","DOIUrl":"10.1016/j.molp.2024.11.002","url":null,"abstract":"Aromatic rice is globally favored for its distinctive scent, which not only increases its nutritional value but also enhances its economic importance. However, apart from 2-acetyl-1-pyrroline (2-AP), the metabolic basis of aroma remains to be clarified, and the genetic basis of the accumulation of fragrance metabolites is largely unknown. In this study, we revealed 2-AP and fatty acid-derived volatiles (FAVs) as key contributors to rice aroma by combining aroma rating with molecular docking. Using a volatilome-based genome-wide association study, we identified two regulatory genes that determine the natural variation of these fragrance metabolites. Genetic and molecular analyses showed that OsWRKY19 not only enhances fragrance by negatively regulating OsBADH2 but also improves agricultural traits in rice. Furthermore, we revealed that OsNAC021 negatively regulates FAV contents via the lipoxygenase pathway, and its knockout resulted in over-accumulation of grain FAVs without a yield penalty. Collectively, our study not only identifies two key regulators of rice aroma but also provides a compelling example about how to deciphering the genetic regulatory mechanisms that underlie rice fragrance, thereby paving the way for the creation of aromatic rice varieties.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1866-1882"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice. TT1-SCE1 模块整合了泛素化和 SUMOylation，以调控水稻的耐热性。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-16 DOI: 10.1016/j.molp.2024.11.007

Hong-Xiao Yu, Ying-Jie Cao, Yi-Bing Yang, Jun-Xiang Shan, Wang-Wei Ye, Nai-Qian Dong, Yi Kan, Huai-Yu Zhao, Zi-Qi Lu, Shuang-Qin Guo, Jie-Jie Lei, Ben Liao, Hong-Xuan Lin

{"title":"A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice.","authors":"Hong-Xiao Yu, Ying-Jie Cao, Yi-Bing Yang, Jun-Xiang Shan, Wang-Wei Ye, Nai-Qian Dong, Yi Kan, Huai-Yu Zhao, Zi-Qi Lu, Shuang-Qin Guo, Jie-Jie Lei, Ben Liao, Hong-Xuan Lin","doi":"10.1016/j.molp.2024.11.007","DOIUrl":"10.1016/j.molp.2024.11.007","url":null,"abstract":"Heat stress poses a significant threat to grain yield. As an α2 subunit of the 26S proteasome, TT1 has been shown to act as a critical regulator of rice heat tolerance. However, the heat tolerance mechanisms mediated by TT1 remain elusive. In this study, we unveiled that small ubiquitin-like modifier (SUMO)-conjugating enzyme 1 (SCE1), which interacts with TT1 and acts as a downstream component of TT1, is engaged in TT1-mediated 26S proteasome degradation. We showed that SCE1 functions as a negative regulator of heat tolerance in rice, which is associated with its ubiquitination modification. Furthermore, we observed that small heat-shock proteins (sHSPs) such as Hsp24.1 and Hsp40 can undergo SUMOylation mediated by SCE1, leading to increased accumulation of sHSPs in the absence of SCE1. Reducing protein levels of SCE1 significantly enhanced grain yield under high-temperature stress by improving seed-setting rate and rice grain filling capacity. Taken together, these results uncover the critical role of SCE1 in the TT1-mediated heat tolerance pathway by regulating the abundance of sHSPs and SUMOylation, and ultimately modulating rice heat tolerance. These findings underscore the great potential of the TT1-SCE1 module in improving the heat tolerance of crops.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1899-1918"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A metabolic roadmap of waxy corn flavor. 蜡质玉米风味的代谢路线图

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-12-02 Epub Date: 2024-11-12 DOI: 10.1016/j.molp.2024.11.003

Jingyun Luo, Chunmei He, Shijuan Yan, Chenglin Jiang, An Chen, Kun Li, Yongli Zhu, Songtao Gui, Ning Yang, Yingjie Xiao, Shenshen Wu, Fajun Zhang, Tieshan Liu, Juan Wang, Wenjie Huang, Yanhua Yang, Haiyan Wang, Wenyu Yang, Wenqiang Li, Lin Zhuo, Alisdair R Fernie, Junpeng Zhan, Liming Wang, Jianbing Yan

{"title":"A metabolic roadmap of waxy corn flavor.","authors":"Jingyun Luo, Chunmei He, Shijuan Yan, Chenglin Jiang, An Chen, Kun Li, Yongli Zhu, Songtao Gui, Ning Yang, Yingjie Xiao, Shenshen Wu, Fajun Zhang, Tieshan Liu, Juan Wang, Wenjie Huang, Yanhua Yang, Haiyan Wang, Wenyu Yang, Wenqiang Li, Lin Zhuo, Alisdair R Fernie, Junpeng Zhan, Liming Wang, Jianbing Yan","doi":"10.1016/j.molp.2024.11.003","DOIUrl":"10.1016/j.molp.2024.11.003","url":null,"abstract":"As well as being a popular vegetable crop worldwide, waxy corn represents an important amylopectin source, but little is known about its breeding history and flavor characteristics. In this study, through comparative-omic analyses between 318 diverse waxy corn and 507 representative field corn inbred lines we revealed that many metabolic pathways and genes exhibited selection characteristics during the breeding history of waxy corn, contributing to the divergence between waxy and field corn. We showed that waxy corn is not only altered in its glutinous property but also its sweetness, aroma, and palatability are all significantly affected. A substantial proportion (43%) of flavor-related metabolites have pleiotropic effects, affecting both flavor and yield characteristics, and 27% of these metabolites are related to antagonistic outcomes on yield and flavor. Furthermore, through multiple concrete examples, we demonstrated how yield and quality are coordinately or antagonistically regulated at the genetic level. In particular, some sweet molecules, such as DIMBOA and raffinose, which do not participate in the starch biosynthesis pathway, were identified as potential targets for breeding a new type of \"sweet-waxy\" corn. Taken together, our findings shed light on the historical selection of waxy corn and demonstrate the genetic and metabolic basis of waxy corn flavor, collectively providing valuable resources and knowledge for future crop breeding for improved nutritional quality.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1883-1898"},"PeriodicalIF":17.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Transition of NRC4 to Hexameric Resistosome Activates Plant Immunity. NRC4 向六聚体抗性体的结构转变可激活植物免疫。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-25 DOI: 10.1016/j.molp.2024.11.011

M Arif Ashraf, Maryam Rashidzade

引用次数: 0

AI-based protein engineering: A novel strategy for enhancing broad-spectrum plant resistance. 基于人工智能的蛋白质工程：增强广谱植物抗性的新策略。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-05 DOI: 10.1016/j.molp.2024.10.004

Jinhong Yuan, Qianqian Li, Xia Li, Chao Su

引用次数: 0

Architecture of the ATP-driven motor for protein import into chloroplasts. 叶绿体中蛋白质输入的 ATP 驱动马达的结构。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-09-25 DOI: 10.1016/j.molp.2024.09.010

Ning Wang, Jiale Xing, Xiaodong Su, Junting Pan, Hui Chen, Lifang Shi, Long Si, Wenqiang Yang, Mei Li

引用次数: 0

Mirids secrete a TOPLESS targeting protein to enhance JA-mediated defense and gossypol accumulation for antagonizing cotton bollworms on cotton plants. Mirids分泌一种TOPLESS靶向蛋白，以增强JA介导的防御和棉酚积累，从而对抗棉花植株上的棉铃虫。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-09-24 DOI: 10.1016/j.molp.2024.09.008

Yu-Pei Mu, Dian-Yang Chen, Yu-Jie Liu, Ming-Yu Zhu, Xian Zhang, Yin Tang, Jia-Ling Lin, Mu-Yang Wang, Xiao-Xia Shangguan, Xiao-Ya Chen, Chengshu Wang, Ying-Bo Mao

{"title":"Mirids secrete a TOPLESS targeting protein to enhance JA-mediated defense and gossypol accumulation for antagonizing cotton bollworms on cotton plants.","authors":"Yu-Pei Mu, Dian-Yang Chen, Yu-Jie Liu, Ming-Yu Zhu, Xian Zhang, Yin Tang, Jia-Ling Lin, Mu-Yang Wang, Xiao-Xia Shangguan, Xiao-Ya Chen, Chengshu Wang, Ying-Bo Mao","doi":"10.1016/j.molp.2024.09.008","DOIUrl":"10.1016/j.molp.2024.09.008","url":null,"abstract":"Most coexisting insect species exhibit stunted growth compared to individual species on plants. This phenomenon reflects an interspecific antagonism drawing extensive attention, while the underlying mechanisms remain largely uncharacterized. Mirids (Apolygus lucorum) and cotton bollworms (Helicoverpa armigera) are two common cotton pests. We identified a secretory protein, ASP1, from the oral secretion of mirids, found in the nucleus of mirid-infested cotton leaves. ASP1 specifically targets the transcriptional co-repressor TOPLESS (TPL) and inhibits NINJA-mediated recruitment of TPL, promoting plant defense response and gossypol accumulation in cotton glands. ASP1-enhanced defense inhibits the growth of cotton bollworms on cotton plants, while having limited impact on mirids. The mesophyll-feeding characteristic allows mirids to avoid most cotton glands, invalidating cotton defense. Our investigation reveals the molecular mechanism by which mirids employ cotton defense to selectively inhibit the feeding of cotton bollworms.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1687-1701"},"PeriodicalIF":17.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142350553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NemaTox: Targeting root-knot nematodes through plastid-based Bt delivery. NemaTox：通过以质体为基础的 Bt 给药技术瞄准根结线虫。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-04 DOI: 10.1016/j.molp.2024.10.002

Alison C Blundell, Shahid Siddique

引用次数: 0

A maize WAK-SnRK1α2-WRKY module regulates nutrient availability to defend against head smut disease. 玉米WAK-SnRK1α2-WRKY模块调节养分供应以抵御头烟粉病。

IF 17.1 1区生物学

Molecular Plant Pub Date : 2024-11-04 Epub Date: 2024-10-01 DOI: 10.1016/j.molp.2024.09.013

Qianqian Zhang, Qianya Xu, Nan Zhang, Tao Zhong, Yuexian Xing, Zhou Fan, Mingzhu Yan, Mingliang Xu

{"title":"A maize WAK-SnRK1α2-WRKY module regulates nutrient availability to defend against head smut disease.","authors":"Qianqian Zhang, Qianya Xu, Nan Zhang, Tao Zhong, Yuexian Xing, Zhou Fan, Mingzhu Yan, Mingliang Xu","doi":"10.1016/j.molp.2024.09.013","DOIUrl":"10.1016/j.molp.2024.09.013","url":null,"abstract":"Obligate biotrophs depend on living hosts for nutrient acquisition to complete their life cycle, yet the mechanisms by which hosts restrict nutrient availability to pathogens remain largely unknown. The fungal pathogen Sporisorium reilianum infects maize seedlings and causes head smut disease in inflorescences at maturity, while a cell wall-associated kinase, ZmWAK, provides quantitative resistance against it. In this study, we demonstrate that S. reilianum can rapidly activate ZmWAK kinase activity, which is sustained by the 407th threonine residue in the juxtamembrane domain, enabling it to interact with and phosphorylate ZmSnRK1α2, a conserved sucrose non-fermenting-related kinase α subunit. The activated ZmSnRK1α2 translocates from the cytoplasm to the nucleus, where it phosphorylates and destabilizes the transcription factor ZmWRKY53. The reduced ZmWRKY53 abundance leads to the downregulation of genes involved in transmembrane transport and carbohydrate metabolism, resulting in nutrient starvation for S. reilianum in the apoplast. Collectively, our study uncovers a WAK-SnRK1α2-WRKY53 signaling module in maize that conveys phosphorylation cascades from the plasma membrane to the nucleus to confer plant resistance against head smut in maize, offering new insights and potential targets for crop disease management.","PeriodicalId":19012,"journal":{"name":"Molecular Plant","volume":" ","pages":"1654-1671"},"PeriodicalIF":17.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0