Targeted Protein Degradation and Protein-condensate Degradation for Plant Science and Crop Breeding.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ruixia Niu, Ming Luo, Qing Wen, Yifan Xiong, Hua Dang, Guoyong Xu
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Abstract

Gene expression can be modulated at the DNA, RNA, or protein level, with targeted protein degradation (TPD) representing a well-established and effective strategy for directly manipulating protein function. TPD enables selective elimination of proteins, protein condensates or organelles by co-opting cellular degradation pathways-such as the ubiquitin-proteasome system, autophagy, or endocytosis-via induced proximity mechanisms. While TPD has had transformative impacts in biomedical research over the past two decades, its application in plant science has lagged behind. This gap stems from the sequential dominance of RNA interference and CRISPR technologies, as well as the complexity and cost of implementing chemical, macromolecular, and recombinant degrader platforms in plants. The recent development of genetically encoded chimeric protein degraders (GE-CPDs) offers a timely and promising alternative. These transgene-based systems provide a plant-adaptable, precise, tunable, and conditional means to control endogenous protein levels, opening new avenues for studying dynamic biological processes and engineering complex traits in crops. As genome engineering technologies continue to advance, GE-CPDs are poised to become a versatile and scalable platform for both basic plant biology and agricultural innovation. In this review, we highlight five key opportunities-Selective-Targeting, Co-Targeting, Organelle-Targeting, Conditional-Targeting, and Synthetic-Engineering (SCOCS)-that illustrate the emerging importance of TPD technologies, particularly GE-CPDs, in advancing plant science. We argue that the field is now well-positioned to harness the full potential of TPD for next-generation crop improvement.

靶向蛋白降解和凝析蛋白降解在植物科学和作物育种中的应用。
基因表达可以在DNA, RNA或蛋白质水平上进行调节,其中靶向蛋白质降解(TPD)代表了直接操纵蛋白质功能的一种成熟而有效的策略。TPD通过诱导接近机制,通过选择细胞降解途径(如泛素-蛋白酶体系统、自噬或内吞),选择性消除蛋白质、蛋白质凝聚物或细胞器。虽然TPD在过去二十年中对生物医学研究产生了变革性影响,但其在植物科学中的应用却滞后。这种差距源于RNA干扰和CRISPR技术的顺序优势,以及在植物中实施化学、大分子和重组降解平台的复杂性和成本。近年来,基因编码嵌合蛋白降解物(GE-CPDs)的发展提供了一个及时而有前途的替代方案。这些基于转基因的系统提供了一种植物适应性的、精确的、可调的和有条件的手段来控制内源蛋白水平,为研究动态生物过程和作物复杂性状的工程设计开辟了新的途径。随着基因组工程技术的不断进步,ge - cpd将成为基础植物生物学和农业创新的通用和可扩展的平台。在这篇综述中,我们强调了五个关键的机会——选择性靶向、共同靶向、细胞器靶向、条件靶向和合成工程(SCOCS)——这说明了TPD技术,特别是GE-CPDs在推进植物科学方面的重要性。我们认为,该领域现在处于有利地位,可以利用TPD的全部潜力进行下一代作物改良。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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