Structural basis of auxin recognition and transport in plant influx carrier AUX1

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Huiwen Chen, Junping Fan, Cheng Chi, Jun Zhao, Di Wu, Xiaoguang Lei, Xing Wang Deng, Daohua Jiang
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引用次数: 0

Abstract

Auxin regulates many aspects of plant growth and development, featuring polar auxin transport mediated by auxin efflux and influx carriers. AUX1 is the prominent auxin importer that actively takes up natural and synthetic auxins. However, the precise mechanisms by which AUX1 recognizes and transports auxin remain elusive. Here, we describe the cryo-electron microscopy structures of Arabidopsis thaliana AUX1 in apo and auxin-bound forms, revealing the structural basis for auxin recognition. AUX1 assumes the LeuT-like fold in an inward conformation. The auxin analogue 2,4-D is recognized by polar residues in the middle of AUX1. We identify a putative cation site in AUX1, which plays a role in stabilizing the inward-facing conformation. His249 undergoes a large conformational shift, and mutation of it completely abolished transport activity, suggesting a crucial role of His249 in AUX1 gating. Together, this study provides a structural foundation for a deeper comprehension of auxin influx by AUX1-like carriers.
植物内流载体AUX1中生长素识别和转运的结构基础
生长素调节植物生长发育的许多方面,包括生长素外排和内流载体介导的极地生长素运输。AUX1是生长素的重要输入源,积极吸收天然和合成的生长素。然而,AUX1识别和运输生长素的确切机制尚不清楚。本文描述了拟南芥AUX1在载脂蛋白和生长素结合形式下的低温电镜结构,揭示了生长素识别的结构基础。AUX1在内向构象中呈leut样折叠。生长素类似物2,4- d被AUX1中间的极性残基识别。我们在AUX1中发现了一个假定的阳离子位点,它在稳定内向构象中起作用。His249发生了巨大的构象变化,其突变完全破坏了转运活性,表明His249在AUX1门控中起着至关重要的作用。总之,该研究为深入理解aux1样载体的生长素内流提供了结构基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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