Hormonal regulation of cell fate plasticity of xylem-pole-pericycle lineage in Arabidopsis roots.

Abstract

In Arabidopsis roots, xylem-pole-pericycle (XPP) cells exhibit dual cell fates by contributing to both lateral root (LR) and cambium formation. Despite the significant progress in understanding these processes individually, the mechanism deciding between these two fates and its contribution on root architecture and secondary growth remain unknown. In this study, we combined lineage tracing with molecular genetics to study the regulation of fate plasticity of XPP cell lineage. We showed that developmentally arrested lateral root primordium (LRP) that fails to emerge as a lateral root gradually obtains cambium identity, thus contributing to secondary growth. Conversely, procambium identity within XPP cells can be reverted to LR identity when simulated by auxin, a key player in LR development. This competence for auxin-induced LR formation from XPP cells, termed LR potency, however, decreases as the root matures. We found that key cambium regulators play critical roles in shaping LR potency not only by promoting cambium identity and activation but also by inhibiting LR formation. Consistently, corresponding mutants with impaired cambium activity display broader LR potency. Moreover, cytokinins, essential players in cambium development, facilitate the identity transition of LRP to cambium and reduce LR potency through key cambium regulators. Taken together, these findings highlight the inherent fate plasticity of XPP cell lineage and elucidate how plant hormones influence root architecture and secondary growth through balancing the two cell fates of XPP cells.