Molecular Breeding最新文献

{"title":"The role of Exo70s in plant defense against pathogens and insect pests and their application for crop breeding.","authors":"Chunxue Xu, Jing Zhang, Wenqian Li, Jianping Guo","doi":"10.1007/s11032-025-01539-3","DOIUrl":"10.1007/s11032-025-01539-3","url":null,"abstract":"<p><p>Plant diseases caused by pathogens and pests lead to crop losses, posing a threat to global food security. The secretory pathway is an integral component of plant defense. The exocyst complex regulates the final step of the secretory pathway and is thus essential for secretory defense. In the last decades, several subunits of the exocyst complex have been reported to be involved in plant defense, especially Exo70s. This comprehensive review focuses on the functions of the exocyst Exo70s in plant immunity, particularly in recognizing pathogen and pest signatures. We discussed Exo70's interactions with immune receptors and other immune-related proteins, its symbiotic relationships with microbes, and its role in non-host resistance. Finally, we discussed the future engineering breeding of crops with resistance to pathogens and pests based on our current understanding of Exo70s.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 2","pages":"17"},"PeriodicalIF":2.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic regions associated with spot blotch resistance in elite barley breeding populations.","authors":"Dipika Roy, Eric Dinglasan, Ryan Fowler, Greg Platz, Reg Lance, Lisle Synman, Jerome Franckowiak, Lee Thomas Hickey, Kai Voss-Fels, Hannah Robinson","doi":"10.1007/s11032-025-01537-5","DOIUrl":"10.1007/s11032-025-01537-5","url":null,"abstract":"<p><p>Spot blotch (SB), a prevalent foliar disease of barley, is caused by the hemibiotrophic fungal pathogen <i>Bipolaris sorokiniana</i>. Predominately occurring in humid growing regions worldwide, SB can result in yield losses of up to 30%. Genetic resistance remains the most effective strategy for disease management; however, most Australian barley cultivars exhibit susceptibility despite the previous identification of major resistance loci. This study investigates the genetic architecture underlying spot blotch resistance within an Australian barley breeding program. Resistance was assessed at both the seedling and adult growth stages using a single conidial isolate (SB61) across two consecutive years. A total of 337 barley lines were genotyped with 16,824 polymorphic DArT-seq™ markers. Two mapping approaches were employed: a single-marker genome-wide association study (GWAS) and a haplotype-based local genomic estimated breeding values (Local GEBV) approach. Both methodologies identified two major resistance-associated regions on chromosomes 3H and 7H, effective across growth stages. Additionally, the haplotype-based Local GEBV approach revealed resistance-associated regions on 1H, 3H, and 6H that were not detected by GWAS. Haplotype stacking analysis underscored the critical role of the 7H region for adult-plant resistance when combined with other resistance haplotypes, suggesting significant gene-by-gene interactions and highlighting the complex, quantitative nature of spot blotch resistance. This research confirms the presence of key resistance loci within Australian barley breeding populations, provides novel insight into the genetic architecture of spot blotch resistance, and emphasises the potential to enhance resistance through haplotype stacking and whole-genome prediction approaches.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01537-5.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 2","pages":"16"},"PeriodicalIF":2.6,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changyou 801, a variety bred for high-oleic-acid rapeseed.","authors":"Jinghua Zhao, Ying Huang, Jinsong Xu, Xuekun Zhang, Lingli Xie, Benbo Xu","doi":"10.1007/s11032-025-01536-6","DOIUrl":"10.1007/s11032-025-01536-6","url":null,"abstract":"","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"15"},"PeriodicalIF":2.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11724809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MaGA20ox2f, an OsSD1 homolog, regulates flowering time and fruit yield in banana.","authors":"Wei Zhao, Xiaoxuan Sun, Shaoping Wu, Shuofan Wu, Chunhua Hu, Heqiang Huo, Guiming Deng, Ou Sheng, Fangcheng Bi, Weidi He, Tongxin Dou, Tao Dong, Chunyu Li, Siwen Liu, Huijun Gao, Chunlong Li, Ganjun Yi, Qiaosong Yang","doi":"10.1007/s11032-024-01523-3","DOIUrl":"10.1007/s11032-024-01523-3","url":null,"abstract":"<p><p>Previous studies illustrated that two banana GA20 oxidase2 (MaGA20ox2) genes, <i>Ma04g15900</i> and <i>Ma08g32850</i>, are implicated in controlling banana growth and development; however, the biological function of each gene remains unknown. Ma04g15900 protein (termed MaGA20ox2f in this article) is the closest homolog to the Rice SD1 (encoded by 'green revolution gene', <i>OsSD1</i>) in the banana genome. The expression of <i>MaGA20ox2f</i> is confined to leaves, peduncles, fruit peels, and pulp. Knockout of <i>MaGA20ox2f</i> by CRISPR/Cas9 led to late flowering and low-yielding phenotypes. The flowering time of <i>ΔMaGA20ox2f</i> #1 and <i>∆MaGA20ox2f</i> #2 lines was delayed approximately by 61 and 58 days, respectively, while fruit yield decreased by 81.13% and 76.23% compared to wild type under normal conditions. The endogenous levels of downstream products of GA20 oxidase, GA15 and GA20, were significantly reduced in <i>∆MaGA20ox2f</i> mutant shoots and fruits, but bioactive GA1 was only significantly reduced in the mutant fruits. Quantitative proteomics analysis identified 118 up-regulated proteins and 309 down-regulated proteins in both <i>ΔMaGA20ox2f</i> #1 and <i>∆MaGA20ox2f</i> #2 lines, compared to wild type, with the down-regulated proteins primarily associated with photosynthesis, porphyrin and chlorophyll metabolism. The decreased chlorophyll contents in <i>ΔMaGA20ox2f</i> #1 and <i>∆MaGA20ox2f</i> #2 lines corroborated the findings of the proteomics data. We propose that photosynthesis inhibition caused by lower chlorophyll contents in <i>ΔMaGA20ox2f</i> mutant leaves and GA1 deficiency in <i>ΔMaGA20ox2f</i> mutant fruits may be the two critical reasons contributing to the late flowering and low-yielding phenotypes of <i>ΔMaGA20ox2f</i> mutants.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01523-3.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"12"},"PeriodicalIF":2.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Speed breeding advancements in safflower (<i>Carthamus tinctorius</i> L.): a simplified and efficient approach for accelerating breeding programs.","authors":"Omar Gaoua, Mehmet Arslan, Samuel Obedgiu","doi":"10.1007/s11032-024-01530-4","DOIUrl":"10.1007/s11032-024-01530-4","url":null,"abstract":"<p><p>This study investigated the potential of extended irradiation combined with immature embryo culture techniques to accelerate generation advancements in safflower (<i>Carthamus tinctorius</i> L.) breeding programs. We developed an efficient speed breeding method by applying light-emitting diodes (LEDs) that emit specific wavelengths, alongside the in vitro germination of immature embryos under controlled environmental conditions. The experimental design for light treatments followed a 2 × 4 completely randomized factorial design with four replications, incorporating two safflower varieties, Remzibey-05 and Dinçer, and four LED treatments (white, full-spectrum, red + blue + white, and control). A lighting regimen of 22 h of light and 2 h of darkness was applied for all the LED treatments, whereas the control received 18 h of light and 6 h of darkness. Additionally, the immature embryo culture experiment used a 2 × 2 × 4 factorial arrangement, assessing two safflower cultivars, two media types, and four embryo developmental stages, with three replications. The parameters evaluated included plant height, branch number, seed number per plant, seed number per head, time to flower initiation, time to 50% flowering, time to harvest, and germination percentage of in vitro cultured immature embryos at various developmental stages. The harvest time among the light treatments ranged from 50.62 to 73.12 days, with the shortest time achieved under the red + blue + white LED combination and the longest under the control treatment. The plant height, number of seeds per plant, and number of seeds per head were highest under the full-spectrum LED, control and red + blue + white LED combinations, respectively. Immature embryos rescued at 10 days post-pollination presented a 57% germination rate, with an increasing trend in germination as the number of days post-pollination increased. The germination rates did not significantly differ across varieties or hormone treatments. This study demonstrated the potential to achieve six generations per year by combining prolonged illumination with targeted LED lighting and immature embryo culture techniques. These findings provide valuable insights for optimizing safflower growth and development and advancing speed breeding in controlled environments.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"13"},"PeriodicalIF":2.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating genomic selection and speed breeding for Fusarium head blight resistance in wheat using stochastic simulations.","authors":"Vinay Kumar Reddy Nannuru, Jon Arne Dieseth, Morten Lillemo, Theodorus H E Meuwissen","doi":"10.1007/s11032-024-01527-z","DOIUrl":"10.1007/s11032-024-01527-z","url":null,"abstract":"<p><p>Genomic selection-based breeding programs offer significant advantages over conventional phenotypic selection, particularly in accelerating genetic gains in plant breeding, as demonstrated by simulations focused on combating Fusarium head blight (FHB) in wheat. FHB resistance, a crucial trait, is challenging to breed for due to its quantitative inheritance and environmental influence, leading to slow progress using conventional breeding methods. Stochastic simulations in our study compared various breeding schemes, incorporating genomic selection (GS) and combining it with speed breeding, against conventional phenotypic selection. Two datasets were simulated, reflecting real-life genotypic data (MASBASIS) and a simulated wheat breeding program (EXAMPLE). Initially a 20-year burn-in phase using a conventional phenotypic selection method followed by a 20-year advancement phase with three GS-based breeding programs (GSF2F8, GSF8, and SpeedBreeding + GS) were evaluated alongside over a conventional phenotypic selection method. Results consistently showed significant increases in genetic gain with GS-based programs compared to phenotypic selection, irrespective of the selection strategies employed. Among the GS schemes, SpeedBreeding + GS consistently outperformed others, generating the highest genetic gains. This combination effectively minimized generation intervals within the breeding cycle, enhancing efficiency. This study underscores the advantages of genomic selection in accelerating breeding gains for wheat, particularly in combating FHB. By leveraging genomic information and innovative techniques like speed breeding, breeders can efficiently select for desired traits, significantly reducing testing time and costs associated with conventional phenotypic methods.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01527-z.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"14"},"PeriodicalIF":2.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11717775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linkage and association analysis to identify wheat pre-harvest sprouting resistance genetic regions and develop KASP markers.","authors":"Pengbo Song, Yueyue Li, Xiaoxiao Wang, Xin Wang, Feng Zhou, Aoyan Zhang, Wensha Zhao, Hailong Zhang, Zeyuan Zhang, Haoyang Li, Huiling Zhao, Kefeng Song, Yuanhang Xing, Daojie Sun","doi":"10.1007/s11032-024-01526-0","DOIUrl":"10.1007/s11032-024-01526-0","url":null,"abstract":"<p><p>Pre-harvest sprouting (PHS) of wheat (<i>Triticum aestivum</i> L.) is one of the complex traits that result in rainfall-dependent reductions in grain production and quality worldwide. Breeding new varieties and germplasm with PHS resistance is of great importance to reduce this problem. However, research on markers and genes related to PHS resistance is limited, especially in marker-assisted selection (MAS) wheat breeding. To this end, we studied PHS resistance in recombinant inbred line (RIL) population and in 171 wheat germplasm accessions in different environments and genotyped using the wheat Infinium 50 K/660 K SNP array. Quantitative trait loci (QTL) mapping and genome-wide association studies (GWAS) identified 59 loci controlling PHS. Upon comparison with previously reported QTL affecting PHS, 16 were found to be new QTL, and the remaining 43 loci were co-localized with QTL from previous studies. We also pinpointed 12 candidate genes within these QTL intervals that share functional similarities with genes previously known to influence PHS resistance. In addition, we developed and validated two kompetitive allele-specific PCR (KASP) markers within the chromosome 7B region identified by linkage analysis. These QTL, candidate genes, and the KASP marker identified in this study have the potential to improve PHS resistance of wheat, and they may enhance our understanding of the genetic basis of PHS resistance, thus being useful for MAS breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01526-0.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"11"},"PeriodicalIF":2.6,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707105/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying the role of cellulase gene <i>CsCEL20</i> upon the infection of <i>Xanthomonas citri</i> subsp. <i>citri</i> in citrus.","authors":"Yi Li, Huijie Lou, Hongyan Fu, Hanying Su, Chenxing Hao, Jianming Luo, Nan Cai, Yan Jin, Jian Han, Ziniu Deng, Yunlin Cao, Xianfeng Ma","doi":"10.1007/s11032-024-01531-3","DOIUrl":"10.1007/s11032-024-01531-3","url":null,"abstract":"<p><p>Citrus canker is a devastating disease caused by <i>Xanthomonas citri</i> subsp. <i>citri</i> (<i>Xcc</i>), which secretes the effector PthA4 into host plants to trigger transcription of the susceptibility gene <i>CsLOB1</i>, resulting in pustule formation. However, the molecular mechanism underlying CsLOB1-mediated susceptibility to <i>Xcc</i> remains elusive. This study identified <i>CsCEL20</i> as a target gene positively regulated by CsLOB1. Cell expansion and cell wall degradation were observed in sweet orange leaves after <i>Xcc</i> infection. A total of 69 cellulase genes were retrieved within the <i>Citrus sinensis</i> genome, comprising 40 endoglucanase genes and 29 glucosidase genes. Transcriptomic analysis revealed that expression levels of <i>CsCEL8</i>, <i>CsCEL9</i>, <i>CsCEL20,</i> and <i>CsCEL26</i> were induced by <i>Xcc</i> invasion in sweet orange leaves, but not in the resistant genotype Citron C-05. Among them, <i>CsCEL20</i> exhibited the highest expression level, with an over 430-fold increase following <i>Xcc</i> infection. Additionally, RT-qPCR analysis confirmed that <i>CsCEL20</i> expression was induced in susceptible genotypes (Sweet orange, Danna citron, Lemon) upon <i>Xcc</i> invasion, but not in resistant genotypes (Citron C-05, Aiguo citron, American citron). A Single-Nucleotide Polymorphism (SNP) at -423 bp was identified in the <i>CEL20</i> promoters and exhibits a difference between eight susceptible citrus genotypes and three resistant ones. Moreover, <i>CsCEL20</i> expression was upregulated in <i>CsLOB1</i>-overexpression transgenic lines compared to the wild type. Dual-luciferase reporter assays indicated that CsLOB1 can target the -505 bp to -168 bp region of <i>CsCEL20</i> promoter to trans-activate its expression. These findings suggest that <i>CsCEL20</i> may function as a candidate gene for citrus canker development and may be a promising target for biotechnological breeding of <i>Xcc</i>-resistant citrus genotypes.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01531-3.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"10"},"PeriodicalIF":2.6,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11704107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the <i>arl1</i> locus controlling leaf rolling and its application in maize breeding.","authors":"Meng Yang, Aihua Huang, Renlai Wen, Shuyun Tian, Runxiu Mo, Ruining Zhai, Xue Gong, Xueyin He, Faqiao Li, Xiaohong Yang, Kaijian Huang, Wenkang Chen, Chenglin Zou","doi":"10.1007/s11032-024-01534-0","DOIUrl":"10.1007/s11032-024-01534-0","url":null,"abstract":"<p><p>Increasing planting density is one of the most important strategies for generating higher maize yields. Moderate leaf rolling decreases mutual shading of leaves and increases the photosynthesis of the population and hence increases the tolerance for high-density planting. Few genes that control leaf rolling in maize have been identified, however, and their applicability for breeding programs remains unclear. Here we identified a maize <i>abaxially rolled leaf1</i> (<i>arl1</i>) mutant with extreme abaxially rolled leaves and found that the size of the bulliform cells within the adaxial leaf blade surface increased in the <i>arl1</i> mutant. Bulk segregation analysis mapping in an F₂ population derived from a single cross between <i>arl1</i> and inbred line Gui18421 with normal leaves identified the <i>arl1</i> locus on chromosome 2. Sequential fine-mapping delimited the <i>arl1</i> locus to a 233.56-kb genomic interval containing three candidate genes. Sequence alignment between <i>arl1</i> and Gui18421 identified an 8-bp insertion in the coding region of <i>Zm00001eb082500</i>, which led to a frame shift causing premature transcription termination in <i>arl1</i> mutant. Meanwhile, both deep sequencing and Sanger sequencing showed that <i>Zm00001eb082520</i> was present in Gui18421 but was absent in <i>arl1</i>. A pair of near isogenic lines (NILs) carrying the Gui18421 allele (NIL^Gui18421) and the <i>arl1</i> allele (NIL ^<i>arl1</i> ) were developed, and the leaves of NIL ^<i>arl1</i> plants had greater light transmission and photosynthetic rate in the middle and lower canopy than did those of NIL^Gui18421 plants under high-density planting. Furthermore, NIL ^<i>arl1</i> had a higher seed setting rate, more kernels per ear, and an increased kernel weight per ear than NIL^Gui18421, and the grain yield of NIL ^<i>arl1</i> was not affected as the planting density increased, suggesting that the <i>arl1</i> locus can be used for genetic improvement of high-density planting tolerance. Taken together, the identification of <i>arl1</i> and evaluation of yield-related traits for NIL^Gui18421 and NIL ^<i>arl1</i> provide an excellent target for future maize improvement.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01534-0.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"9"},"PeriodicalIF":2.6,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11700961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the genetic architecture of soybean tofu quality traits.","authors":"Cleo A Döttinger, Kim A Steige, Volker Hahn, Kristina Bachteler, Willmar L Leiser, Xintian Zhu, Tobias Würschum","doi":"10.1007/s11032-024-01529-x","DOIUrl":"10.1007/s11032-024-01529-x","url":null,"abstract":"<p><p>Tofu is a popular soybean (<i>Glycine max</i> (L.) Merr.) food with a long tradition in Asia and rising popularity worldwide, including Central Europe. Due to the labour-intensive phenotyping procedures, breeding for improved tofu quality is challenging. Therefore, our objective was to unravel the genetic architecture of traits relevant for tofu production in order to assess the potential of marker-assisted selection and genomic selection in breeding for these traits. To this end, we performed QTL mapping with 188 genotypes from a biparental mapping population. The population was evaluated in a two-location field trial, and tofu was produced in the laboratory to evaluate tofu quality. We identified QTL for all investigated agronomic and quality traits, each explaining between 6.40% and 27.55% of the genotypic variation, including the most important tofu quality traits, tofu yield and tofu hardness. Both traits showed a strong negative correlation (<i>r</i> = -0.65), and consequently a pleiotropic QTL on chromosome 10 was found with opposite effects on tofu hardness and tofu weight, highlighting the need to balance selection for both traits. Four QTL identified for tofu hardness jointly explained 68.7% of the genotypic variation and are possible targets for QTL stacking by marker-assisted selection. To exploit also small-effect QTL, genomic selection revealed moderate to high mean prediction accuracies for all traits, ranging from 0.47 to 0.78. In conclusion, inheritance of tofu quality traits is highly quantitative, and both marker-assisted selection and genomic selection present valuable tools to advance tofu quality by soybean breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01529-x.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"8"},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}