Molecular Breeding最新文献

{"title":"Molecular mechanisms underlying the signal perception and transduction during seed germination","authors":"Huibin Xu, Fuxiang Wang, Rebecca Njeri Damari, Xiaofeng Chen, Zhongyuan Lin","doi":"10.1007/s11032-024-01465-w","DOIUrl":"https://doi.org/10.1007/s11032-024-01465-w","url":null,"abstract":"<p>QuerySeed germination is a vital step in the life cycle of a plant, playing a significant role in seedling establishment and crop yield potential. It is also an important factor in the conservation of plant germplasm resources. This complex process is influenced by a myriad of factors, including environmental conditions, the genetic makeup of the seed, and endogenous hormones. The perception of these environmental signals triggers a cascade of intricate signal transduction events that determine whether a seed germinates or remains dormant. Despite considerable progress in uncovering the molecular mechanisms governing these processes, many questions remain unanswered. In this review, we summarize the current progress in the molecular mechanisms underlying the perception of environmental signals and consequent signal transduction during seed germination, and discuss questions that need to be addressed to better understand the process of seed germination and develop novel strategies for germplasm improvement.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"48 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mapping and candidate gene identification of two major quantitative trait loci associated with silique length in oilseed rape (Brassica napus L.)","authors":"","doi":"10.1007/s11032-024-01464-x","DOIUrl":"https://doi.org/10.1007/s11032-024-01464-x","url":null,"abstract":"<h3>Abstract</h3> <p>Rapeseed is a significant global source of plant oil. Silique size, particularly silique length (SL), impacts rapeseed yield. SL is a typical quantitative trait controlled by multiple genes. In our previous study, we constructed a DH population of 178 families known as the 158A-SGDH population. In this study, through SL QTL mapping, we identified twenty-six QTL for SL across five replicates in two environments. A QTL meta-analysis revealed eight consensus QTL, including two major QTL: <em>cqSL.A02-1</em> (11.32–16.44% of PVE for SL), and <em>cqSL.C06-1</em> (10.90–11.95% of PVE for SL). Based on biparental resequencing data and microcollinearity analysis of target regions in <em>Brassica napus</em> and <em>Arabidopsis</em>, we identified 11 candidate genes at <em>cqSL.A02-1</em> and 6 candidate genes at <em>cqSL.C06-1</em>, which are potentially associated with silique development. Furthermore, transcriptome analysis of silique valves from both parents on the 14th, 21st, and 28th days after pollination (DAP) combined with gene function annotation revealed three significantly differentially expressed genes at <em>cqSL.A02-1</em>, <em>BnaA02G0058500ZS</em>, <em>BnaA02G0060100ZS</em>, and <em>BnaA02G0060900ZS</em>. Only the gene <em>BnaC06G0283800ZS</em> showed significant differences in parental transcription at <em>cqSL.C06-1</em>. Two tightly linked insertion-deletion markers for the <em>cqSL.A02-1</em> and <em>cqSL.C06-1</em> loci were developed. Using these two QTL, we generated four combinations: A02^SGDH284C06^158A, A02^SGDH284C06^SGDH284, A02^158AC06^158A, and A02^158AC06^SGDH284. Subsequent analysis identified an ideal QTL combination, A02^158AC06^SGDH284, which exhibited the longest SL of this type, reaching 6.06 ± 0.10 cm, significantly surpassing the other three combinations. The results will provide the basis for the cloning of SL-related genes of rapeseed, along with the development of functional markers of target genes and the breeding of rapeseed varieties.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"21 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140166884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene pyramiding of ZmGLK36 and ZmGDIα-hel for rough dwarf disease resistance in maize","authors":"Gongjian Li, Zhennan Xu, Jianjun Wang, Chunhua Mu, Zhiqiang Zhou, Mingshun Li, Zhuanfang Hao, Degui Zhang, Hongjun Yong, Jienan Han, Xinhai Li, Jiqiang Zhao, Jianfeng Weng","doi":"10.1007/s11032-024-01466-9","DOIUrl":"https://doi.org/10.1007/s11032-024-01466-9","url":null,"abstract":"<p>Maize rough dwarf disease (MRDD) caused by pathogenic viruses in the genus <i>Fijivirus</i> in the family Reoviridae is one of the most destructive diseases in maize. The pyramiding of effective resistance genes into maize varieties is a potential approach to reduce the damage resulting from the disease. Two major quantitative trait loci (QTLs) (<i>qMrdd2</i> and <i>qMrdd8</i>) have been previously identified. The resistance genes <i>ZmGLK36</i> and <i>ZmGDIα-hel</i> have also been cloned with the functional markers Indel-26 and IDP25K, respectively. In this study, <i>ZmGLK36</i> and <i>ZmGDIα-hel</i> were introgressed to improve MRDD resistance of maize lines (Zheng58, Chang7-2, B73, Mo17, and their derived hybrids Zhengdan958 and B73 × Mo17) via marker-assisted selection (MAS). The converted lines and their derived hybrids, carrying one or two genes, were evaluated for MRDD resistance using artificial inoculation methods. The double-gene pyramiding lines and their derived hybrids exhibited increased resistance to MRDD compared to the monogenic lines and the respective hybrids. The genetic backgrounds of the converted lines were highly similar (90.85–98.58%) to the recurrent parents. In addition, agronomic trait evaluation demonstrated that pyramiding lines with one or two genes and their derived hybrids were not significantly different from the recurrent parents and their hybrids under nonpathogenic stress, including period traits (tasseling, pollen shedding, and silking), yield traits (ear length, grain weight per ear and 100-kernel weight) and quality traits (protein and starch content). There were differences in plant architecture traits between the improved lines and their hybrids. This study illustrated the successful development of gene pyramiding for improving MRDD resistance by advancing the breeding process.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"22 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140166887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel allelic variations in <i>Tannin1</i> and <i>Tannin2</i> contribute to tannin absence in sorghum.","authors":"Wenbin Zhang, Ryan Benke, Xiao Zhang, Huawen Zhang, Cunyuan Zhao, Yu Zhao, Ying Xu, Hailian Wang, Shubing Liu, Xianran Li, Yuye Wu","doi":"10.1007/s11032-024-01463-y","DOIUrl":"10.1007/s11032-024-01463-y","url":null,"abstract":"<p><p>Sorghum is an important food crop commonly used for brewing, feed, and bioenergy. Certain genotypes of sorghum contain high concentrations of condensed tannins in seeds, which are beneficial, such as protecting grains from herbivore bird pests, but also impair grain quality and digestibility. Previously, we identified <i>Tannin1</i> and <i>Tannin2</i>, each with three recessive causal alleles, regulate tannin absence in sorghum. In this study, via characterizing 421 sorghum accessions, we further identified three novel recessive alleles from these two genes. The <i>tan1-d</i> allele contains a 12-bp deletion at position 659 nt and the <i>tan1-e</i> allele contains a 10-bp deletion at position 771 nt in <i>Tannin1</i>. The <i>tan2-d</i> allele contains a C-to-T transition, which results in a premature stop codon before the bHLH domain in <i>Tannin2</i>, and was predominantly selected in China. We further developed KASP assays targeting these identified recessive alleles to efficiently genotype large populations. These studies provide new insights in sorghum domestication and convenient tools for breeding programs.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01463-y.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 3","pages":"24"},"PeriodicalIF":3.1,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10942951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic dissection and identification of stripe rust resistance genes in the wheat cultivar Lanhangxuan 121, a cultivar selected from a space mutation population","authors":"","doi":"10.1007/s11032-024-01461-0","DOIUrl":"https://doi.org/10.1007/s11032-024-01461-0","url":null,"abstract":"<h3>Abstract</h3> <p>Stripe rust is a devastating disease of wheat worldwide. Chinese wheat cultivar Lanhangxuan 121 (LHX121), selected from an advanced line L92-47 population that had been subjected to space mutation breeding displayed a consistently higher level of resistance to stipe rust than its parent in multiple field environments. The aim of this research was to establish the number and types of resistance genes in parental lines L92-47 and LHX121 using separate segregating populations. The first population developed from a cross between LHX121 and susceptible cultivar Xinong 822 comprised 278 F_2:3 lines. The second validation population comprised 301 F_2:3 lines from a cross between L92-47 and susceptible cultivar Xinong 979. Lines of two population were evaluated for stripe rust response at three sites during the 2018–2020 cropping season. Affymetrix 660 K SNP arrays were used to genotype the lines and parents. Inclusive composite interval mapping detected QTL <em>QYrLHX.nwafu-2BS</em>, <em>QYrLHX.nwafu-3BS,</em> and <em>QYrLHX.nwafu-5BS</em> for resistance in all three environments. Based on previous studies and pedigree information, <em>QYrLHX.nwafu-2BS</em> and <em>QYrLHX.nwafu-3BS</em> were likely to be <em>Yr27</em> and <em>Yr30</em> that are present in the L92-47 parent. <em>QYrLHX.nwafu-5BS</em> (<em>YrL121</em>) detected only in LHX121 was mapped to a 7.60 cM interval and explained 10.67–22.57% of the phenotypic variation. Compared to stripe rust resistance genes previously mapped to chromosome 5B, <em>YrL121</em> might be a new adult plant resistance QTL. Furthermore, there were a number of variations signals using 35 K SNP array and differentially expressed genes using RNA-seq between L92-47 and LHX121 in the <em>YrL121</em> region, indicating that they probably impair the presence and/or function of <em>YrL121</em>.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"113 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140026249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild species rice OsCERK1DY-mediated arbuscular mycorrhiza symbiosis boosts yield and nutrient use efficiency in rice breeding","authors":"Ruicai Han, Zhou Yang, Chunquan Wang, Shan Zhu, Guoping Tang, Xianhua Shen, Deqiang Duanmu, Yangrong Cao, Renliang Huang","doi":"10.1007/s11032-024-01459-8","DOIUrl":"https://doi.org/10.1007/s11032-024-01459-8","url":null,"abstract":"<p>Meeting the ever-increasing food demands of a growing global population while ensuring resource and environmental sustainability presents significant challenges for agriculture worldwide. Arbuscular mycorrhizal symbiosis (AMS) has emerged as a potential solution by increasing the surface area of a plant's root system and enhancing the absorption of phosphorus, nitrogen nutrients, and water. Consequently, there is a longstanding hypothesis that rice varieties exhibiting more efficient AMS could yield higher outputs at reduced input costs, paving the way for the development of Green Super Rice (GSR). Our prior research study identified a variant, <i>OsCERK1</i>^<i>DY</i>, derived from Dongxiang wild-type rice, which notably enhanced AMS efficiency in the rice cultivar \"ZZ35.\" This variant represents a promising gene for enhancing yield and nutrient use efficiency in rice breeding. In this study, we conducted a comparative analysis of biomass, crop growth characteristics, yield attributes, and nutrient absorption at varying soil nitrogen levels in the rice cultivar \"ZZ35\" and its chromosome single-segment substitution line, \"GJDN1.\" In the field, GJDN1 exhibited a higher AM colonization level in its roots compared with ZZ35. Notably, GJDN1 displayed significantly higher effective panicle numbers and seed-setting rates than ZZ35. Moreover, the yield of GJDN1 with 75% nitrogen was 14.27% greater than the maximum yield achieved using ZZ35. At equivalent nitrogen levels, GJDN1 consistently outperformed ZZ35 in chlorophyll (Chl) content, dry matter accumulation, major nutrient element accumulation, N agronomic efficiency (NAE), N recovery efficiency (NRE), and N partial factor productivity (NPFP). The performance of <i>OsCERK1</i>^<i>DY</i> overexpression lines corroborated these findings. These results support a model wherein the heightened level of AMS mediated by <i>OsCERK1</i>^<i>DY</i> contributes to increased nitrogen, phosphorus, and potassium accumulation. This enhancement in nutrient utilization promotes higher fertilizer efficiency, dry matter accumulation, and ultimately, rice yield. Consequently, the <i>OsCERK1</i>^<i>DY</i> gene emerges as a robust candidate for improving yield, reducing fertilizer usage, and facilitating a transition towards greener, lower-carbon agriculture.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"19 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Truncation of the calmodulin binding domain in rice glutamate decarboxylase 4 (OsGAD4) leads to accumulation of γ-aminobutyric acid and confers abiotic stress tolerance in rice seedlings","authors":"Nadia Akter, Ummey Kulsum, Mohammad Moniruzzaman, Norito Yasuda, Kazuhito Akama","doi":"10.1007/s11032-024-01460-1","DOIUrl":"https://doi.org/10.1007/s11032-024-01460-1","url":null,"abstract":"<p>GABA (Gamma-aminobutyric acid) is a non-protein amino acid widely known as major inhibitory neurotransmitter. It is synthesized from glutamate via the enzyme glutamate decarboxylase (GAD). GAD is ubiquitous in all organisms, but only plant GAD has ability to bind Ca²⁺/calmodulin (CaM). This kind of binding suppresses the auto-inhibition of Ca²⁺/calmodulin binding domain (CaMBD) when the active site of GAD is unfolded resulting in stimulated GAD activity. <i>OsGAD4</i> is one of the five <i>GAD</i> genes in rice genome. It was confirmed that <i>OsGAD4</i> has ability to bind to Ca²⁺/CaM. Moreover, it exhibits strongest expression against several stress conditions among the five <i>OsGAD</i> genes. In this study, CRISPR/Cas9-mediated genome editing was performed to trim the coding region of CaMBD from the <i>OsGAD4</i> gene, to remove its autoinhibitory function. DNA sequence analysis of the genome edited rice plants revealed the truncation of CaMBD (216 bp). Genome edited line (#14–1) produced 11.26 mg GABA/100 g grain, which is almost nine-fold in comparison to wild type. Short deletion in the coding region for CaMBD yielded in mutant (#14–6) with lower GABA content than wild type counterpart. Abiotic stresses like salinity, flooding and drought significantly enhanced GABA accumulation in #14–1 at various time points compared to wild-type and #14–6 under the same stress conditions. Moreover, upregulated mRNA expression in vegetative tissues seems correlated with the stress-responsiveness of <i>OsGAD4</i> when exposed to the above-mentioned stresses. Stress tolerance of <i>OsGAD4</i> genome edited lines was evidenced by the higher survival rate indicating the gene may induce tolerance against abiotic stresses in rice. This is the first report on abiotic stress tolerance in rice modulated by endogenous GABA.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tetraploid-dominated cytochimera developed from a natural bud mutant of the nonapomictic mandarin variety ‘Orah’","authors":"","doi":"10.1007/s11032-024-01456-x","DOIUrl":"https://doi.org/10.1007/s11032-024-01456-x","url":null,"abstract":"<h3>Abstract</h3> <p>Nonapomictic citrus tetraploids are desirable in citrus breeding for the production of triploid, seedless varieties, and polyploid rootstocks. However, only a few lines have been reported, and they were all generated using chemical methods. A 2x + 4 × cytochimera of the nonapomictic citrus variety ‘Orah’ mandarin, which developed from a bud mutant, was found due to its morphology differing from that of diploid plants and characterised via ploidy analysis combining flow cytometry and chromosome observation. The chimaera was stable, and there were 1.86–1.90 times as tetraploid cells as diploid cells. Anatomical structure observation revealed that the ‘Orah’ chimaera may be a periclinal chimaera with diploid cells in the L1 layer and tetraploid cells in the L2 and L3 layers. The chimaera showed some typical traits of polyploid plants, including thicker shoots, wider and thicker leaves, larger flowers and fruits, and fewer but larger seeds in fruits than in diploid plants. Almost all the seeds of the chimaera were monoembryonic. Most of the self-pollinated progenies of the chimaera were identified as tetraploids, and some triploid, pentaploid, and hexaploid plants were found. As a female, the chimaera produced allotriploids when crossed with Australian finger lime. In addition, 6 plants developed from polyembryonic seeds of the chimaera were identified as sexual tetraploid progenies with low-level recombinant genomes. Therefore, the ‘Orah’ 2x + 4 × chimaera can be used as a female parent to produce hybrid triploid and tetraploid citrus plants with high efficiency. Identification of the chimaera demonstrated that tetraploid citrus plants, especially nonapomictic varieties, can be generated from shoot bud mutants.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"2015 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139949339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accumulation of mutations in the AP2 homoeologs causes suppression of anther extrusion with altered spike and culm development in hexaploid wheat","authors":"Agetha Bigie Nanape, Takao Komatsuda, Katsuyuki Kakeda","doi":"10.1007/s11032-024-01458-9","DOIUrl":"https://doi.org/10.1007/s11032-024-01458-9","url":null,"abstract":"<p>Cleistogamy or closed flowering is a widely used trait in barley (<i>Hordeum vulgare</i>) breeding because it reduces the risk of fungal infection in florets at anthesis. Cleistogamy in barley is caused by a point mutation within the microRNA172 (miR172) target site of the <i>Cly1</i> gene, which encodes the Apetala2 (AP2) transcription factor. Because cleistogamy is not apparent in cultivars of hexaploid wheat (<i>Triticum aestivum</i>), a strategy to develop cleistogamous wheat was proposed by inducing point mutations in all three <i>AP2</i> homoeologs, which are the wheat orthologs of barley <i>Cly1</i>. In this study, we investigated the effects of miR172 target site mutations on wheat cleistogamy using double mutants by combining three previously obtained mutant alleles (<i>AP2-A1</i>, <i>D1</i> and <i>D2</i>) in a near-isogenic background. The <i>AP2-D2</i> allele had the greatest effect on reducing the anther extrusion rate and lodicule size compared with the other two mutant alleles. The double mutant containing the <i>AP2-A1</i> and <i>AP2-D2</i> alleles had a much greater suppression of anther extrusion by reducing the lodicule size than the single <i>AP2-D2</i> mutant, suggesting cumulative effects of the two mutant alleles. In addition, both single and double mutants exhibited compact spikes and shorter plant heights due to reduced rachis and culm internodes in the upper parts. The presence or absence of the wild-type <i>AP2-B</i> homoeolog had no significant effect on phenotype. This study provides insights into the cumulative effects of mutant <i>AP2</i> alleles in suppressing open flowering and provides a basis for further research on the development of complete cleistogamy in hexaploid wheat.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"254 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139949230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PbrCSP1, a pollen tube–specific cold shock domain protein, is essential for the growth and cold resistance of pear pollen tubes","authors":"","doi":"10.1007/s11032-024-01457-w","DOIUrl":"https://doi.org/10.1007/s11032-024-01457-w","url":null,"abstract":"<h3>Abstract</h3> <p>Cold shock domain proteins (CSPs), initially identified in <em>Escherichia coli</em>, have been demonstrated to play a positive role in cold resistance. Previous studies in wheat, rice, and <em>Arabidopsis</em> have indicated the functional conservation of CSPs in cold resistance between bacteria and higher plants. However, the biological functions of PbrCSPs in pear pollen tubes, which represent the fragile reproductive organs highly sensitive to low temperature, remain largely unknown. In this study, a total of 22 CSPs were identified in the seven Rosaceae species, with a focus on characterizing four PbrCSPs in pear (<em>Pyrus bretschneideri</em> Rehder). All four PbrCSPs were structurally conserved and responsive to the abiotic stresses, such as cold, high osmotic, and abscisic acid (ABA) treatments. PbrCSP1, which is specifically expressed in pear pollen tubes, was selected for further research. PbrCSP1 was localized in both the cytoplasm and nucleus. Suppressing the expression of <em>PbrCSP1</em> significantly inhibited the pollen tube growth in vitro. Conversely, overexpression of <em>PbrCSP1</em> promoted the growth of pear pollen tubes under the normal condition and, notably, under the cold environment at 4 °C. These findings highlight an essential role of PbrCSP1 in facilitating the normal growth and enhancing cold resistance in pear pollen tubes.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"10 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139919706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}