Improvement of the outcrossing ability of CMS lines by pyramiding QTLs for SER.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-02-08 eCollection Date: 2025-02-01 DOI:10.1007/s11032-025-01545-5

Quanya Tan, Zhenpeng Gan, Lin Shao, Liang Xiong, Xin Luan, Weifeng Yang, Haitao Zhu, Suhong Bu, Guifu Liu, Zupei Liu, Shaokui Wang, Guiquan Zhang

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引用次数: 0

Abstract

The stigma exsertion rate (SER) is a key factor in improving the outcrossing ability of cytoplasmic male sterility (CMS) lines in rice. In previous studies, we identified 18 SER-QTLs and developed some SER-QTL pyramiding lines (PLs). In this study, 4QL-1 and 4QL-2 were selected from these PLs and crossed with CMS maintainer lines H211B and H212B, respectively, to develop two new CMS maintainer lines, H221B and H222B, and their CMS lines H221A and H222A. The SER of H221B and H222B were 74.7% and 73.1%, respectively, reaching a high SER level. Compared with CMS maintainer lines, the CMS lines consistently exhibited higher SER, which may be related to the delayed flowering time of the CMS lines. Filed experiments showed that outcrossing seed-setting rates of H221A and H222A were significantly higher than those of the original CMS lines, which meets the requirements for hybrid rice seed production. These results confirm that SER is a key factor in enhancing rice outcrossing ability. Our findings demonstrate that pyramiding SER-QTLs is an effective strategy for improving rice SER and increasing outcrossing seed-setting rate.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01545-5.

查看原文本刊更多论文

利用SER qtl提高CMS系的异交能力。

柱头外露率（SER）是提高水稻细胞质雄性不育系异交能力的关键因素。在之前的研究中，我们鉴定了18个SER-QTL，并开发了一些SER-QTL金字塔系（PLs）。本研究从这些PLs中选择4QL-1和4QL-2，分别与CMS保持系H211B和H212B杂交，培育出2个新的CMS保持系H221B和H222B及其CMS系H221A和H222A。H221B和H222B的SER分别为74.7%和73.1%，SER水平较高。与CMS保持系相比，CMS系SER持续较高，这可能与CMS系开花时间延迟有关。田间试验表明，H221A和H222A的异交结实率显著高于原CMS系，满足杂交水稻制种要求。这些结果证实了SER是提高水稻异交能力的关键因素。结果表明，构建SER- qtl是提高水稻SER水平和提高异交结实率的有效策略。补充资料：在线版本包含补充资料，下载地址：10.1007/s11032-025-01545-5。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.