抗坏血酸过氧化物酶2 （APX2）基因的过表达提高了玉米的抗旱性。

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-02-15 eCollection Date: 2025-02-01 DOI:10.1007/s11032-025-01548-2

Bai Gao, Yiran Wang, Jing Qu, Ming Miao, Yang Zhao, Siyan Liu, Shuyan Guan, Yiyong Ma

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引用次数: 0

摘要

玉米是全球主要粮食作物，对粮食安全至关重要。近年来，气候和其他非生物压力导致全球干旱频繁发生。抗坏血酸过氧化物酶（APX）在抗坏血酸-谷胱甘肽循环中起重要作用。在干旱胁迫下，APX能有效清除植物产生的活性氧（ROS），维持生物的正常生长发育。本研究成功扩增了apx相关基因，并通过表达分析筛选了ZmAPX2基因。构建了pCAMBIA3301-ZmAPX2-Bar和pCXB053-ZmAPX2-Bar植物表达载体，并将其转化为玉米自交系H120。通过T2代阳性玉米幼苗的表型特征、生理生化指标和成熟期农艺性状分析植株的耐旱性。结果表明，提高APX2基因表达可通过降低活性氧含量来增强玉米抗旱性。本研究为探索玉米抗旱新种质及其抗旱机制奠定了基础。补充资料：在线版本包含补充资料，下载地址：10.1007/s11032-025-01548-2。

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The overexpression of ascorbate peroxidase 2 (APX2) gene improves drought tolerance in maize.

Maize, a primary global food crop, is crucial for food security. In recent years, climatic and other abiotic stresses have led to frequent global droughts. Ascorbate peroxidase (APX) plays a vital role in the ascorbate-glutathione cycle. Under drought stress, APX effectively scavenges reactive oxygen species (ROS) produced by plants and maintains the normal growth and development of organisms. This study successfully amplified APX-related genes, and the ZmAPX2 gene was screened using expression analysis. pCAMBIA3301-ZmAPX2-Bar and pCXB053-ZmAPX2-Bar plant expression vectors were constructed and transformed into the maize inbred line H120. Drought tolerance of plants was analyzed by phenotypic characteristics, physiological and biochemical indices in T₂ generation positive maize seedlings as well as agronomic traits at maturity. Results indicate that boosting APX2 gene expression enhances maize drought resistance by reducing ROS content. This research underpins the exploration of new drought-tolerant maize germplasm and resistance mechanisms.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01548-2.

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.