Molecular Breeding最新文献

{"title":"Recent progress in the understanding of Citrus Huanglongbing: from the perspective of pathogen and citrus host.","authors":"Kun Yang, Bin Hu, Wang Zhang, Tao Yuan, Yuantao Xu","doi":"10.1007/s11032-024-01517-1","DOIUrl":"10.1007/s11032-024-01517-1","url":null,"abstract":"<p><p>Citrus Huanglongbing (HLB) is a devastating disease spread by citrus psyllid, causing severe losses to the global citrus industry. The transmission of HLB is mainly influenced by both the pathogen and the citrus psyllid. The unculturable nature of the HLB bacteria (<i>Candidatus</i> Liberibacter asiaticus, <i>C</i>Las) and the susceptibility of all commercial citrus varieties made it extremely difficult to study the mechanisms of resistance and susceptibility. In recent years, new progress has been made in understanding the virulence factors of <i>C</i>Las as well as the defense strategies of citrus host against the attack of <i>C</i>Las. This paper reviews the recent advances in the pathogenic mechanisms of <i>C</i>Las, the screening of agents targeting the <i>C</i>Las, including antimicrobial peptides, metabolites and chemicals, the citrus host defense response to <i>C</i>Las, and strategies to enhance citrus defense. Future challenges that need to be addressed are also discussed.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"77"},"PeriodicalIF":2.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving cabbage resistance to <i>Sclerotinia sclerotiorum</i> via crosses with <i>Brassica incana</i>.","authors":"Qinfei Li, Jiaqin Yang, Xiaoyun Liu, Jiabing Wu, Ao Peng, Jun Si, Xuesong Ren, Jiaqin Mei, Wei Qian, Honghao Lv, Zujun Tang, Hongyuan Song","doi":"10.1007/s11032-024-01513-5","DOIUrl":"https://doi.org/10.1007/s11032-024-01513-5","url":null,"abstract":"<p><p>Cabbage is a widely cultivated leafy vegetable, but head rot disease caused by the fungus <i>Sclerotina sclerotiorum</i> can seriously reduce its yield and quality. There are currently not any cabbage varieties that are completely immune to the disease, but its wild relative <i>Brassica incana</i> is very resistant. In this study, cabbage resistance was improved by backcrossing a highly resistant <i>B. incana</i> accession (C01) with a susceptible cabbage cultivar (F416). Although C01 lacks a leafy head formation, highly resistant plants appeared in the fourth backcrossing generation (BC₄F₁) that had a similar leafy head to F416. The individuals with strong resistance were purified by self-pollination. Inbred lines that maintained a relatively stable resistance at BC₄F₃ were developed and had significantly higher resistance to <i>S. sclerotiorum</i> than F416. In addition, hybrids created from a cross between of BC₄F₃ and E2 had higher resistances to <i>S. sclerotiorum</i> and similar agronomic characteristics to Xiyuan 4. The results demonstrated that new F416 lines that are resistant to <i>S. sclerotiorum</i> can be developed, and that these lines could be used to create new cabbage varieties with superior head rot resistance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01513-5.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"76"},"PeriodicalIF":2.6,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11538228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the genetic architecture of the reciprocal interspecific hybrids derived from <i>Chrysanthemum dichrum</i> and <i>C. nankingense</i>.","authors":"Zhaowen Lu, Jiangshuo Su, Honghong Fan, Xuefeng Zhang, Haibin Wang, Zhiyong Guan, Weimin Fang, Fadi Chen, Fei Zhang","doi":"10.1007/s11032-024-01518-0","DOIUrl":"10.1007/s11032-024-01518-0","url":null,"abstract":"<p><p>Chrysanthemums are versatile ornamental plants, and improving leaf and flower traits is an important breeding objective. Distant hybridization is a powerful method for plant breeding and genetic improvement, whereas the genetic basis in interspecific F₁ progeny of chrysanthemums needs to be better understood for breeding purposes. In this study, the leaf and floral traits of the 273 reciprocal interspecific F₁ hybrids of diploid <i>C. dichrum</i> (YSJ) and <i>C. nankingense</i> (JHN) were analyzed along with their SNP-derived genetic structure to elucidate the influence of differences in genetic background between the parents on the hybrid performance. We then performed a genome-wide association analysis (GWAS) to reveal the investigated traits' genomic loci and candidate genes. Considerable phenotypic variation (8.81% ~ 55.78%) and heterosis with transgressive segregation in both directions were observed in the reciprocal progenies. We observed a higher level of phenotypic variation in JHN × YSJ rather than in YSJ × JHN. Also, a significant reciprocal effect was observed for most examined traits. Based on the SNP data, we separated the hybrid progenies into three groups (I, II, and III), albeit imperfectly dependent on the cross directions, except for some reciprocal hybrids clustering into group II. Group I from YSJ × JHN and Group III from YSJ × JHN differed with contrasting <i>F</i> _<i>ST</i> and π ratios, indicating the genetic changes in the reciprocal populations. The outcome of GWAS via the IIIVmrMLM method detected 339 significant quantitative trait nucleotides (QTNs) and 40 suggestive QTNs, and the phenotypic variation explained by a single QTN ranged from 0.26% to 7.42%. Within 100 kb upstream and downstream of the important QTNs, we discovered 49 known genes and 39 new candidate genes for the investigated leaf and floral traits. Our study provides profound insights into the genetic architecture of reciprocal hybrid progenies of chrysanthemum species, facilitating future breeding activities.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01518-0.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"75"},"PeriodicalIF":2.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zheyouza 59, a rapeseed variety with superior resistance and excellent yield.","authors":"Ying Fu, Huasheng Yu, Quanfeng Zhang, Huabing Lu, Daomin Liu, Yaofeng Zhang","doi":"10.1007/s11032-024-01514-4","DOIUrl":"10.1007/s11032-024-01514-4","url":null,"abstract":"","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"74"},"PeriodicalIF":2.6,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11532309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and fine mapping of <i>Double-grain</i> (<i>Dgs</i>) mutant in sorghum [<i>Sorghum bicolor</i> (L.) Moench].","authors":"Shanshan Liang, Shichen Zhou, Zhiben Yi, Yanbao Tian, Mao Qin, Jiahan Wang, Youchuan Hu, Dan Liang, Siju Zhang, Xuan Ma, Yunhai Li, Weijiang Luan","doi":"10.1007/s11032-024-01511-7","DOIUrl":"10.1007/s11032-024-01511-7","url":null,"abstract":"<p><p>The sorghum inflorescence is consisted of sessile (SS) and pedicellate spikelets (PS). Commonly, only SS could produce seeds and each spikelet produces one single seed. Here, we identified a sorghum mutant, named <i>Double-grain</i> (<i>Dgs</i>), which can produce twin seeds in each pair of glumes. We characterized the developmental process of inflorescence in <i>Dgs</i> and Jinliang 5 (Jin5, a single-seeded variety) using scanning electron microscope (SEM). The results showed that at the stamen and pistil differentiation stage, <i>Dgs</i> could develop two sets of stamens and carpels in one sessile floret, which resulted in twin-seeded phenotype in <i>Dgs</i>. Two F₂ mapping populations derived from the cross between Jin5 and <i>Dgs</i>, and BTx622B and <i>Dgs</i>, were constructed, respectively. The genetic analysis showed that <i>Dgs</i> trait was controlled by a single dominant gene. Through bulk segregation analysis with whole-genome sequencing (BSA-seq) and linkage analysis, <i>Dgs</i> locus was delimited into a region of around 210-kb on chromosome 6, between the markers <i>SSR24</i> and <i>SSR47</i>, which contained 32 putative genes. Further analysis indicated that <i>Sobic.006G249000</i> or <i>Sobic.006G249100</i> may be responsible for the twin-seeded phenotype. This result will be useful for map-based cloning of the <i>Dgs</i> gene and for marker-assisted breeding for increased grain number per panicle in sorghum.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01511-7.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 11","pages":"73"},"PeriodicalIF":2.6,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11496449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolongation of seed viability and grain quality in rice by editing <i>OsLOX1</i> using CRISPR/Cas9.","authors":"Changling Mou, Yaping Chen, Ping Zhang, Qikai Tong, Ziyan Zhu, Tengfei Ma, Ping Wang, Kai Fu, Cheng Chen, Yunshuai Huang, Fulin Zhang, Qixian Hao, Min Zhang, Shijia Liu, Ling Jiang, Jianmin Wan","doi":"10.1007/s11032-024-01506-4","DOIUrl":"https://doi.org/10.1007/s11032-024-01506-4","url":null,"abstract":"<p><p>Deterioration of rice (<i>Oryza sativa</i> L.) affects grain quality and seed viability during storage. Lipoxygenase (LOX), a key enzyme in lipid metabolism, directly affects the rate of ageing. Here, we found that knock-out of lipoxygenase gene <i>OsLOX</i>1 by CRISPR/Cas9 delayed loss of seed viability and quality. Transcriptome analysis showed that during storage, <i>OsLOX1</i> affected transcription of multiple genes, including genes related to lipid metabolism and antioxidant pathways such as phosphatase and acetaldehyde dehydrogenase, which may regulate the seed storability. The genes significantly down- and up-regulated only in Ningjing 4 after NA for 13 months and 3 days of AA suggesting that <i>OsLOX1</i> likely promoted seed viability in rice by balancing ageing and storage related genes, and regulated the seed storability through the amino acid synthesis and metabolic pathways. Moreover, knock-out of <i>OsLOX1</i> without CRISPR/Cas9 not only improved the seed viability, but also had little impact on agronomic traits. More importantly, the <i>OsLOX1</i> knock-out lines were approved in 2019 (Agricultural Foundation of China Report No. 770). Collectively, our study showed that knock-out of <i>OsLOX1</i> is beneficial for prolongation of seed viability and can be directly applied to agricultural production.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01506-4.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 10","pages":"72"},"PeriodicalIF":2.6,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and application of Key Allele-Specific PCR (KASP) molecular markers for assessing apple fruit crispness.","authors":"Yao Xiao, Ling Wu, Baoan Wang, Manyu Zhang, Qi Pan, Linfeng Xian, Jing Sheng, Mengbo Yan, Jingxian Jin, Rui Zhang, Jing Zhang, Qiulin Zeng, Tianzhong Li, Wei Li","doi":"10.1007/s11032-024-01509-1","DOIUrl":"10.1007/s11032-024-01509-1","url":null,"abstract":"<p><p>Crispness stands as a pivotal criterion in assessing apple texture, widely cherished by consumers. Yet, owing to its multifaceted nature, crispness remains a formidable challenge in artificial enhancement efforts. To expedite the early and precise evaluation of apple crispness, this study centered on a hybrid population derived from 'Fuji' and 'Pink Lady' cultivars, showcasing segregating crispness traits. We conducted measurements of flesh water content, cellular anatomical morphology, and employed a texture analyzer to assess mechanical properties of the offspring flesh. Integrating these three dimensions, we conducted a comprehensive analysis of quantitative characteristics of apple crispness, juxtaposed with sensory evaluation. Utilizing BSA-seq technology, we scrutinized extreme phenotypic individuals, revealing QTL loci intricately linked to the aforementioned dimensions, and subsequently developed Key Allele-Specific PCR (KASP) markers. These markers underwent validation in hybrid populations of 'Hanfu' x 'Pink Lady' and 'Hanfu' x 'Honey Crisp'. Our findings underscored significant correlations between mechanical properties, water content, and cell size with crispness. Higher mechanical properties and water content, alongside smaller cell size, correlated with firmer flesh texture; moderate mechanical properties, and elevated water content and cell size, with crisper texture; whereas lower mechanical properties, water content, and cell size implied softer flesh.The study yielded KASP markers effectively reflecting flesh mechanical properties (SNP_24399345), water content (SNP_8667563), and cell size (SNP_15566229). Comprehensive analysis of these markers identified CC-CC-TT as an effective identifier of soft flesh individuals; while GG-TC-TT and GG-CC-TT combinations better represented individuals with harder flesh. The Crunchy subclass could be discerned by combinations of GG-TC-TC, GG-TC-CC, GG-TT-TC, and GG-TT-CC. These findings furnish effective molecular markers for the genetic enhancement of apple crispness, bearing significant implications for the cultivation of novel apple varieties.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01509-1.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 10","pages":"71"},"PeriodicalIF":2.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11467153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the genetic mechanisms of chilling requirement for bud endodormancy release in deciduous fruit trees.","authors":"Weihan Zhang, Liao Liao, Baoxiong Wan, Yuepeng Han","doi":"10.1007/s11032-024-01510-8","DOIUrl":"10.1007/s11032-024-01510-8","url":null,"abstract":"<p><p>Bud endodormancy in deciduous fruit trees is an adaptive trait evolved by selection for the capacity to survive unfavorable environmental conditions. Deciduous trees require a certain amount of winter chill named chilling requirement (CR) to promote bud endodormancy release. In recent decades, global warming has endangered the chill accumulation in deciduous fruit trees. Developing low-CR cultivars is a practical way to neutralize the effect of climate changes on the cultivation and distribution of deciduous fruit trees. In this review, we focus on the effect of chilling accumulation on bud endodormancy release and the genetic mechanisms underlying the chilling requirement in deciduous fruit trees. Additionally, we put forth a regulatory model for bud endodormancy and provide prospective directions for future research in deciduous fruit trees.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 10","pages":"70"},"PeriodicalIF":2.6,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of <i>ZmPHR1</i> and <i>ZmPHR2</i> under low-phosphate stress in maize.","authors":"Hongmei Hu, Yikai Wang, Haixu Zhong, Binyang Li, Jingxiao Qi, Yarong Wang, Jin Liu, Shuhao Zhang, Haiying Zhang, Bowen Luo, Xiao Zhang, Zhi Nie, Hongkai Zhang, Duojiang Gao, Shiqiang Gao, Dan Liu, Ling Wu, Shibin Gao","doi":"10.1007/s11032-024-01508-2","DOIUrl":"10.1007/s11032-024-01508-2","url":null,"abstract":"<p><p>The PHOSPHATE STARVATION RESPONSE REGULATOR (PHR) plays a crucial regulatory role in plants during the process of responding to phosphate starvation. In this study, we combined reverse genetics and biotechnology to investigate the function of <i>ZmPHR1</i> and <i>ZmPHR2</i>, including proteins containing the Myb_DNA_banding and Myb_CC-LHEQLE structural domains, in maize seedlings. Phylogenetic analysis revealed that <i>ZmPHR1</i> and <i>ZmPHR2</i> have high homology with <i>AtPHR1</i> and <i>OsPHR2</i>, and share the characteristic features of nuclear localisation and transcriptional self-activation. Real-time quantitative PCR analysis showed that low phosphate (Pi) stress significantly induced the expression of <i>ZmPHR1</i> and <i>ZmPHR2</i> in maize seedling stage, and candidate gene association analysis further revealed the close association of these two genes with root traits under Pi stress conditions. Transgenic plants overexpressing <i>ZmPHR1</i> and <i>ZmPHR2</i> in <i>Arabidopsis</i> show a significant increase in lateral root number, fresh weight and total phosphorus accumulation under low-Pi stress. Besides, CHIP-PCR experiments identified target genes involved in hormone regulation, metal ion transport and homeostasis, phosphatase encoding, and photosynthesis, providing new insights into the biological functions of <i>ZmPHR1</i> and <i>ZmPHR2</i>. Furthermore, our study showed that ZmPHR1 interacts with six SPX domain-only proteins (ZmSPXs) in maize, while ZmPHR2 interacts with five of these proteins. <i>ZmPHR1</i> and <i>ZmPHR2</i> expression was repressed in low Pi conditions, but was up-regulated in <i>ZmSPX1</i> knockout material, according to our study of transgenic seedlings overexpressing <i>ZmSPX1</i> in maize. We identified downstream target genes involved in the phosphorus signaling pathway, which are mainly involved in plant-pathogen interactions, ascorbic acid and arabinose metabolism, and ABC transporter proteins, by RNA-seq analysis of transgenic seedlings grown under low Pi stress for 7 days. Collectively, these results provide important clues to elucidate the role and functional significance of <i>ZmPHR1</i> and <i>ZmPHR2</i> under low Pi stress and also provide insights into understand the molecular mechanism of phosphorus homeostasis in maize.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01508-2.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 10","pages":"69"},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11442720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>BnaC09.tfl1</i> controls determinate inflorescence trait in <i>Brassica napus</i>.","authors":"Xutao Zhao, Lingxiong Zan, Niaofei He, Haidong Liu, Xiaorong Xing, Dezhi Du, Guoyong Tang, Kaixiang Li","doi":"10.1007/s11032-024-01503-7","DOIUrl":"10.1007/s11032-024-01503-7","url":null,"abstract":"<p><p>Determinate inflorescence is indeed a pivotal agricultural characteristic in crops, notably impacting the architecture modification of <i>Brassica napus</i> (AACC, 2n = 38). Previous study identified a crucial gene <i>Bnsdt2</i> that encodes the transcription factor <i>BnaC09.TFL1</i> (<i>Terminal Flower 1</i>). Here by two alleles were cloned and sequenced from indeterminate 2982 and determinate 4769, respectively, we found that <i>BnaC09.TFL1</i> harbors two T/C and G/C non-synonymous mutations in exon 1, and contains sixty-six differences in a 1.9 Kb promoter sequence. Subsequently, <i>BnaC09.TFL1</i> was introduced into <i>B. napus</i> 571 line by genetic complementation and overexpression, transgenic plants 571^CTO lines and 571^TClines were all restored to the indeterminate inflorescence. Interestingly, after <i>BnaC09.TFL1</i> was knocked out in 'Westar', transgenic plants Westar^Tcr lines were mutated to determinate inflorescences. Additionally, a NIL-4769 line was constructed to evaluate the effect of <i>BnaC09.TFL1</i> on agronomic traits of <i>Brassica napus</i>, the results demonstrated that <i>BnaC09.tfl1</i> reduced the plant height and increased the branch number and branch thousand grain weight of <i>Brassica napus.</i> Finally, we performed RT-qPCR, GUS staining and subcellular localization experiments to analyze the expression pattern of <i>BnaC09.TFL1</i>, the results showed that the expression of <i>BnaC09.TFL1</i> at shoot apex of NIL-4769 was higher than that of 4769, GUS activity was detected at apical of <i>Arabidopsis thaliana</i> and <b><i>BnC09.TFL1-GFP</i></b> was detected in cell membrane, nucleus and cytoplasm. Our findings provide a firm molecular foundation for the study of rapeseed's molecular mechanism of determinate inflorescence formation, as well as theoretical guidance for the application of determinate inflorescence in rapeseed breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01503-7.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"44 10","pages":"68"},"PeriodicalIF":2.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142350344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}