{"title":"对菊花和杭白菊种间互交杂种遗传结构的见解。","authors":"Zhaowen Lu, Jiangshuo Su, Honghong Fan, Xuefeng Zhang, Haibin Wang, Zhiyong Guan, Weimin Fang, Fadi Chen, Fei Zhang","doi":"10.1007/s11032-024-01518-0","DOIUrl":null,"url":null,"abstract":"<p><p>Chrysanthemums are versatile ornamental plants, and improving leaf and flower traits is an important breeding objective. Distant hybridization is a powerful method for plant breeding and genetic improvement, whereas the genetic basis in interspecific F<sub>1</sub> progeny of chrysanthemums needs to be better understood for breeding purposes. In this study, the leaf and floral traits of the 273 reciprocal interspecific F<sub>1</sub> hybrids of diploid <i>C. dichrum</i> (YSJ) and <i>C. nankingense</i> (JHN) were analyzed along with their SNP-derived genetic structure to elucidate the influence of differences in genetic background between the parents on the hybrid performance. We then performed a genome-wide association analysis (GWAS) to reveal the investigated traits' genomic loci and candidate genes. Considerable phenotypic variation (8.81% ~ 55.78%) and heterosis with transgressive segregation in both directions were observed in the reciprocal progenies. We observed a higher level of phenotypic variation in JHN × YSJ rather than in YSJ × JHN. Also, a significant reciprocal effect was observed for most examined traits. Based on the SNP data, we separated the hybrid progenies into three groups (I, II, and III), albeit imperfectly dependent on the cross directions, except for some reciprocal hybrids clustering into group II. Group I from YSJ × JHN and Group III from YSJ × JHN differed with contrasting <i>F</i> <sub><i>ST</i></sub> and π ratios, indicating the genetic changes in the reciprocal populations. The outcome of GWAS via the IIIVmrMLM method detected 339 significant quantitative trait nucleotides (QTNs) and 40 suggestive QTNs, and the phenotypic variation explained by a single QTN ranged from 0.26% to 7.42%. Within 100 kb upstream and downstream of the important QTNs, we discovered 49 known genes and 39 new candidate genes for the investigated leaf and floral traits. Our study provides profound insights into the genetic architecture of reciprocal hybrid progenies of chrysanthemum species, facilitating future breeding activities.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01518-0.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534950/pdf/","citationCount":"0","resultStr":"{\"title\":\"Insights into the genetic architecture of the reciprocal interspecific hybrids derived from <i>Chrysanthemum dichrum</i> and <i>C. nankingense</i>.\",\"authors\":\"Zhaowen Lu, Jiangshuo Su, Honghong Fan, Xuefeng Zhang, Haibin Wang, Zhiyong Guan, Weimin Fang, Fadi Chen, Fei Zhang\",\"doi\":\"10.1007/s11032-024-01518-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Chrysanthemums are versatile ornamental plants, and improving leaf and flower traits is an important breeding objective. Distant hybridization is a powerful method for plant breeding and genetic improvement, whereas the genetic basis in interspecific F<sub>1</sub> progeny of chrysanthemums needs to be better understood for breeding purposes. In this study, the leaf and floral traits of the 273 reciprocal interspecific F<sub>1</sub> hybrids of diploid <i>C. dichrum</i> (YSJ) and <i>C. nankingense</i> (JHN) were analyzed along with their SNP-derived genetic structure to elucidate the influence of differences in genetic background between the parents on the hybrid performance. We then performed a genome-wide association analysis (GWAS) to reveal the investigated traits' genomic loci and candidate genes. Considerable phenotypic variation (8.81% ~ 55.78%) and heterosis with transgressive segregation in both directions were observed in the reciprocal progenies. We observed a higher level of phenotypic variation in JHN × YSJ rather than in YSJ × JHN. Also, a significant reciprocal effect was observed for most examined traits. Based on the SNP data, we separated the hybrid progenies into three groups (I, II, and III), albeit imperfectly dependent on the cross directions, except for some reciprocal hybrids clustering into group II. Group I from YSJ × JHN and Group III from YSJ × JHN differed with contrasting <i>F</i> <sub><i>ST</i></sub> and π ratios, indicating the genetic changes in the reciprocal populations. The outcome of GWAS via the IIIVmrMLM method detected 339 significant quantitative trait nucleotides (QTNs) and 40 suggestive QTNs, and the phenotypic variation explained by a single QTN ranged from 0.26% to 7.42%. Within 100 kb upstream and downstream of the important QTNs, we discovered 49 known genes and 39 new candidate genes for the investigated leaf and floral traits. 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引用次数: 0
摘要
菊花是用途广泛的观赏植物，改善叶片和花朵的性状是重要的育种目标。远缘杂交是植物育种和遗传改良的有效方法，而菊花种间 F1 代后代的遗传基础则需要进一步了解，以达到育种目的。在本研究中，我们分析了二倍体菊花（C. dichrum，YSJ）和南京菊（C. nankingense，JHN）的 273 个互交种间 F1 杂交种的叶和花性状及其 SNP 衍生遗传结构，以阐明亲本间遗传背景差异对杂种表现的影响。然后，我们进行了全基因组关联分析（GWAS），以揭示所研究性状的基因组位点和候选基因。在互交后代中观察到了相当大的表型变异（8.81% ~ 55.78%）和杂合子双向转性分离。我们观察到 JHN × YSJ 的表型变异水平高于 YSJ × JHN。此外，在大多数考察性状中都观察到了明显的互作效应。根据 SNP 数据，我们将杂交后代分为三组（I、II 和 III），尽管这与杂交方向并不完全相关，但一些互交后代被归入 II 组。来自 YSJ × JHN 的 I 组和来自 YSJ × JHN 的 III 组具有截然不同的 F ST 和 π 比值，表明互交群体的遗传变化。通过IIIVmrMLM方法进行的GWAS结果发现了339个显著的数量性状核苷酸（QTN）和40个提示性QTN，单个QTN解释的表型变异从0.26%到7.42%不等。在重要 QTN 上下游 100 kb 范围内，我们发现了 49 个已知基因和 39 个新的候选基因，这些基因与所研究的叶和花性状有关。我们的研究为菊花品种互交后代的遗传结构提供了深刻的见解，有助于未来的育种活动：在线版本包含补充材料，可查阅 10.1007/s11032-024-01518-0。
Insights into the genetic architecture of the reciprocal interspecific hybrids derived from Chrysanthemum dichrum and C. nankingense.
Chrysanthemums are versatile ornamental plants, and improving leaf and flower traits is an important breeding objective. Distant hybridization is a powerful method for plant breeding and genetic improvement, whereas the genetic basis in interspecific F1 progeny of chrysanthemums needs to be better understood for breeding purposes. In this study, the leaf and floral traits of the 273 reciprocal interspecific F1 hybrids of diploid C. dichrum (YSJ) and C. nankingense (JHN) were analyzed along with their SNP-derived genetic structure to elucidate the influence of differences in genetic background between the parents on the hybrid performance. We then performed a genome-wide association analysis (GWAS) to reveal the investigated traits' genomic loci and candidate genes. Considerable phenotypic variation (8.81% ~ 55.78%) and heterosis with transgressive segregation in both directions were observed in the reciprocal progenies. We observed a higher level of phenotypic variation in JHN × YSJ rather than in YSJ × JHN. Also, a significant reciprocal effect was observed for most examined traits. Based on the SNP data, we separated the hybrid progenies into three groups (I, II, and III), albeit imperfectly dependent on the cross directions, except for some reciprocal hybrids clustering into group II. Group I from YSJ × JHN and Group III from YSJ × JHN differed with contrasting FST and π ratios, indicating the genetic changes in the reciprocal populations. The outcome of GWAS via the IIIVmrMLM method detected 339 significant quantitative trait nucleotides (QTNs) and 40 suggestive QTNs, and the phenotypic variation explained by a single QTN ranged from 0.26% to 7.42%. Within 100 kb upstream and downstream of the important QTNs, we discovered 49 known genes and 39 new candidate genes for the investigated leaf and floral traits. Our study provides profound insights into the genetic architecture of reciprocal hybrid progenies of chrysanthemum species, facilitating future breeding activities.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01518-0.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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