MaGA20ox2f, an OsSD1 homolog, regulates flowering time and fruit yield in banana.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-01-09 eCollection Date: 2025-01-01 DOI:10.1007/s11032-024-01523-3

Wei Zhao, Xiaoxuan Sun, Shaoping Wu, Shuofan Wu, Chunhua Hu, Heqiang Huo, Guiming Deng, Ou Sheng, Fangcheng Bi, Weidi He, Tongxin Dou, Tao Dong, Chunyu Li, Siwen Liu, Huijun Gao, Chunlong Li, Ganjun Yi, Qiaosong Yang

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引用次数: 0

Abstract

Previous studies illustrated that two banana GA20 oxidase2 (MaGA20ox2) genes, Ma04g15900 and Ma08g32850, are implicated in controlling banana growth and development; however, the biological function of each gene remains unknown. Ma04g15900 protein (termed MaGA20ox2f in this article) is the closest homolog to the Rice SD1 (encoded by 'green revolution gene', OsSD1) in the banana genome. The expression of MaGA20ox2f is confined to leaves, peduncles, fruit peels, and pulp. Knockout of MaGA20ox2f by CRISPR/Cas9 led to late flowering and low-yielding phenotypes. The flowering time of ΔMaGA20ox2f #1 and ∆MaGA20ox2f #2 lines was delayed approximately by 61 and 58 days, respectively, while fruit yield decreased by 81.13% and 76.23% compared to wild type under normal conditions. The endogenous levels of downstream products of GA20 oxidase, GA15 and GA20, were significantly reduced in ∆MaGA20ox2f mutant shoots and fruits, but bioactive GA1 was only significantly reduced in the mutant fruits. Quantitative proteomics analysis identified 118 up-regulated proteins and 309 down-regulated proteins in both ΔMaGA20ox2f #1 and ∆MaGA20ox2f #2 lines, compared to wild type, with the down-regulated proteins primarily associated with photosynthesis, porphyrin and chlorophyll metabolism. The decreased chlorophyll contents in ΔMaGA20ox2f #1 and ∆MaGA20ox2f #2 lines corroborated the findings of the proteomics data. We propose that photosynthesis inhibition caused by lower chlorophyll contents in ΔMaGA20ox2f mutant leaves and GA1 deficiency in ΔMaGA20ox2f mutant fruits may be the two critical reasons contributing to the late flowering and low-yielding phenotypes of ΔMaGA20ox2f mutants.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01523-3.

查看原文本刊更多论文

与OsSD1同源基因MaGA20ox2f调控香蕉开花时间和果实产量。

先前的研究表明，两个香蕉GA20氧化酶2 （MaGA20ox2）基因Ma04g15900和Ma08g32850参与控制香蕉的生长发育；然而，每个基因的生物学功能仍然未知。Ma04g15900蛋白（本文称为MaGA20ox2f）是香蕉基因组中与水稻SD1（由“绿色革命基因”OsSD1编码）最接近的同源物。MaGA20ox2f的表达仅限于叶片、花梗、果皮和果肉。CRISPR/Cas9敲除MaGA20ox2f导致开花晚、产量低的表型。正常条件下，ΔMaGA20ox2f #1和∆MaGA20ox2f #2品系的开花时间分别延迟约61和58 d，单果产量比野生型下降81.13%和76.23%。GA20氧化酶下游产物GA15和GA20的内源水平在突变体（∆MaGA20ox2f）的茎部和果实中显著降低，但GA1的生物活性仅在突变体果实中显著降低。定量蛋白质组学分析发现，与野生型相比，ΔMaGA20ox2f #1和∆maga20ox2f# 2系中有118个蛋白上调，309个蛋白下调，其中下调的蛋白主要与光合作用、卟啉和叶绿素代谢有关。ΔMaGA20ox2f #1和∆maga20ox2f# 2的叶绿素含量下降证实了蛋白质组学数据的发现。我们认为，ΔMaGA20ox2f突变体叶片叶绿素含量低和ΔMaGA20ox2f突变体果实GA1缺乏导致的光合作用抑制可能是导致ΔMaGA20ox2f突变体开花晚、产量低的两个关键原因。补充资料：在线版本提供补充资料，网址为10.1007/s11032-024-01523-3。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.