Linkage and association analysis to identify wheat pre-harvest sprouting resistance genetic regions and develop KASP markers.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-01-07 eCollection Date: 2025-01-01 DOI:10.1007/s11032-024-01526-0

Pengbo Song, Yueyue Li, Xiaoxiao Wang, Xin Wang, Feng Zhou, Aoyan Zhang, Wensha Zhao, Hailong Zhang, Zeyuan Zhang, Haoyang Li, Huiling Zhao, Kefeng Song, Yuanhang Xing, Daojie Sun

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引用次数: 0

Abstract

Pre-harvest sprouting (PHS) of wheat (Triticum aestivum L.) is one of the complex traits that result in rainfall-dependent reductions in grain production and quality worldwide. Breeding new varieties and germplasm with PHS resistance is of great importance to reduce this problem. However, research on markers and genes related to PHS resistance is limited, especially in marker-assisted selection (MAS) wheat breeding. To this end, we studied PHS resistance in recombinant inbred line (RIL) population and in 171 wheat germplasm accessions in different environments and genotyped using the wheat Infinium 50 K/660 K SNP array. Quantitative trait loci (QTL) mapping and genome-wide association studies (GWAS) identified 59 loci controlling PHS. Upon comparison with previously reported QTL affecting PHS, 16 were found to be new QTL, and the remaining 43 loci were co-localized with QTL from previous studies. We also pinpointed 12 candidate genes within these QTL intervals that share functional similarities with genes previously known to influence PHS resistance. In addition, we developed and validated two kompetitive allele-specific PCR (KASP) markers within the chromosome 7B region identified by linkage analysis. These QTL, candidate genes, and the KASP marker identified in this study have the potential to improve PHS resistance of wheat, and they may enhance our understanding of the genetic basis of PHS resistance, thus being useful for MAS breeding.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01526-0.

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小麦收获前发芽抗性遗传区域鉴定和KASP标记开发的连锁关联分析。

小麦（Triticum aestivum L.）收获前发芽（PHS）是导致全球粮食产量和质量因降雨而下降的复杂性状之一。培育具有小灵通抗性的新品种和种质对减少这一问题具有重要意义。然而，对小麦小灵通抗性相关的标记和基因的研究还很有限，特别是在小麦的标记辅助选择育种方面。为此，我们研究了不同环境下重组自交系（RIL）群体和171份小麦种质的小灵通抗性，并利用小麦Infinium 50 K/660 K SNP阵列进行了基因分型。数量性状位点（QTL）定位和全基因组关联研究（GWAS）鉴定出59个控制小灵通的位点。与先前报道的影响小灵通的QTL比较，发现16个为新QTL，其余43个位点与先前研究的QTL共定位。我们还在这些QTL区间内确定了12个候选基因，这些基因与先前已知的影响小灵通抗性的基因具有功能相似性。此外，我们在染色体7B区开发并验证了两个通过连锁分析鉴定的竞争性等位基因特异性PCR （KASP）标记。本研究鉴定的QTL、候选基因和KASP标记具有提高小麦小灵通抗性的潜力，有助于我们进一步了解小灵通抗性的遗传基础，从而为MAS育种提供参考。补充资料：在线版本提供补充资料，网址为10.1007/s11032-024-01526-0。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.