Molecular Breeding最新文献

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Genome-wide analysis of phased small interfering RNAs related to tomato fruit ripening and quality. 与番茄果实成熟和品质相关的阶段性小干扰rna全基因组分析。
IF 3 3区 农林科学
Molecular Breeding Pub Date : 2026-02-14 eCollection Date: 2026-02-01 DOI: 10.1007/s11032-026-01639-8
Santiago Stalder, Agustín Sabbione, Lucas Daurelio, Marcela Dotto
{"title":"Genome-wide analysis of phased small interfering RNAs related to tomato fruit ripening and quality.","authors":"Santiago Stalder, Agustín Sabbione, Lucas Daurelio, Marcela Dotto","doi":"10.1007/s11032-026-01639-8","DOIUrl":"https://doi.org/10.1007/s11032-026-01639-8","url":null,"abstract":"<p><p>Fruit quality and sensorial aspects are key determinants for costumer selection and satisfaction. Main characteristics include texture, color, aroma and taste, which are defined by a wide range of molecular processes that will ultimately determine fruit general appearance. The production of cultivars with optimized metabolite contents is increasingly being explored, in particular through the study of gene regulatory networks that modulate the metabolism of these compounds. There are several types of small RNAs originating from different biosynthesis mechanisms that regulate a large number of genes involved in various processes during plant development and growth. However, reports on their participation in regulatory networks related to qualitative aspects of fruits are scarce. In this work, genome-wide bioinformatics analyses were performed to identify regulatory modules that involve the action of phased secondary small interfering RNAs in different ripening stages of tomato fruits. We identified small RNA regulatory modules related to the acquisition of commercially important traits, such as the development of brightness, color, firmness, and nutritional content, which set the basis for further characterization of phasiRNA-mediated regulation of aspects related to tomato fruit quality improvement and the possibility of using these regulatory RNAs as tools for molecular breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-026-01639-8.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 2","pages":"18"},"PeriodicalIF":3.0,"publicationDate":"2026-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12906421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146207321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fine mapping and verification of a tiller inhibition gene in synthetic hexaploid wheat derivative. 合成六倍体小麦分蘖抑制基因的精细定位与验证。
IF 3 3区 农林科学
Molecular Breeding Pub Date : 2026-02-07 eCollection Date: 2026-02-01 DOI: 10.1007/s11032-026-01640-1
Shuai Hou, Lang Yu, Wenjie Guo, Yuzhou Mou, Haojie Li, Fengcheng Shi, Jian Zhou, Zhiqiang Wang, Yu Lin, Yaxi Liu
{"title":"Fine mapping and verification of a tiller inhibition gene in synthetic hexaploid wheat derivative.","authors":"Shuai Hou, Lang Yu, Wenjie Guo, Yuzhou Mou, Haojie Li, Fengcheng Shi, Jian Zhou, Zhiqiang Wang, Yu Lin, Yaxi Liu","doi":"10.1007/s11032-026-01640-1","DOIUrl":"https://doi.org/10.1007/s11032-026-01640-1","url":null,"abstract":"<p><p>Tiller number is a pivotal agronomic trait governing wheat yield potential, yet its underlying genetic regulation remains insufficiently elucidated. Here, we report the map-based cloning of <i>tin7</i>, a major gene controlling tiller number in wheat. Using near-isogenic lines (NIL-H, high-tillering; NIL-L, low-tillering), we fine-mapped <i>tin7</i>. Eleven polymorphic markers genotyped 10,753 F₂ individuals, <i>tin7</i> was mapped to a 400.61 kb physical interval in Chinese Spring genome, which contained 16 high-confidence genes in Chinese Spring (17 high-confidence genes in emmer wheat). Sequence and expression analyses identified <i>TraesCS2B01G619900</i> (ortholog of emmer wheat <i>TRIDC2BG090810</i>) as the candidate for <i>tin7</i>, with NIL-L harboring a premature stop codon. Functional validation via EMS-induced and CRISPR/Cas9-mediated mutants confirmed its role: EMS mutants showed 67.90-80.25% tiller reduction, while CRISPR/Cas9 mutants in KN199 exhibited a significant reduction (37.31-62.88%). These findings confirm <i>TraesCS2B01G619900</i> is <i>tin7</i>, regulating wheat tillering and providing a valuable genetic resource for yield improvement via molecular breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-026-01640-1.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 2","pages":"17"},"PeriodicalIF":3.0,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12882920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multi-dimensional trait improvement of two unique semi-dwarf Indica rice varieties through combinatorial introgression of five bacterial blight resistance genes and sd1 alleles. 利用5个抗白叶枯病基因和sd1等位基因组合渗入改良两个独特的半矮秆籼稻品种的多维性状。
IF 3 3区 农林科学
Molecular Breeding Pub Date : 2026-02-06 eCollection Date: 2026-02-01 DOI: 10.1007/s11032-026-01633-0
Pritiranjan Sahoo, Kutubuddin A Molla, Pratap Bhattacharyya, Anilkumar Chandrappa, Sutapa Sarkar, Guru Pirasanna Pandi Govindharaj, Lambodar Behera, Pankajini Samal, Motilal Behera, Archana Bal, Nillahit Payra, Debajyoti Roy, Anowar Hossain, Trilochan Mohapatra, Arup K Mukhherjee, Mridul Chakraborti, Meera Kumari Kar
{"title":"Multi-dimensional trait improvement of two unique semi-dwarf Indica rice varieties through combinatorial introgression of five bacterial blight resistance genes and <i>sd1</i> alleles.","authors":"Pritiranjan Sahoo, Kutubuddin A Molla, Pratap Bhattacharyya, Anilkumar Chandrappa, Sutapa Sarkar, Guru Pirasanna Pandi Govindharaj, Lambodar Behera, Pankajini Samal, Motilal Behera, Archana Bal, Nillahit Payra, Debajyoti Roy, Anowar Hossain, Trilochan Mohapatra, Arup K Mukhherjee, Mridul Chakraborti, Meera Kumari Kar","doi":"10.1007/s11032-026-01633-0","DOIUrl":"https://doi.org/10.1007/s11032-026-01633-0","url":null,"abstract":"<p><p>Pooja and Naveen are popular semidwarf rice varieties of eastern and northeastern India. Bacterial blight disease caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) and lodging lead to yield loss in both genotypes. Considering unique racial composition of <i>Xoo</i> in target regions, five resistance genes (<i>Xa4</i>, <i>xa5</i>, <i>Xa7</i>, <i>xa13</i> and <i>Xa21</i>) were pyramided in Naveen using IRBB66 as donor. The near isogenic lines (NILs) showed resistant reaction across growing seasons and against multiple <i>Xoo</i> strains. Although plant height of the NILs were similar to Naveen, several tall and semi-tall recombinants appeared from F<sub>2</sub> generation onwards. Molecular profiling confirmed presence of <i>SD1</i> allele in Naveen, its NILs and the taller recombinant inbred lines (RILs). <i>Xa4</i> gene improved lodging resistance in both semidwarf NILs and the taller RILs. One non-lodging semi-tall RIL with <i>Xa4 + xa5</i> + <i>Xa7 + xa13</i> + <i>Xa21 + SD1</i> allelic combination with substantially higher yield than both parents and significantly higher yield than check genotypes was released as a new variety CR Dhan 326 after blind multilocation trials. The <i>Xa4</i> gene also reduced lodging of <i>SD1SD1</i> RILs developed by crossing CR Dhan 326 with RP-Bio-226 (<i>xa5</i> + <i>xa13</i> + <i>Xa21 + sd1</i>). NILs with <i>Xa4 + xa5</i> + <i>Xa7 + xa13</i> + <i>Xa21</i> genes were also developed in Pooja. However, backcrossing led to irreversible loss of semi-dwarfism and presence of <i>SD1</i> allele was confirmed in Pooja and its NILs. Semidwarf NILs could be developed only after introgression of <i>sd1</i> allele. The <i>Xa4</i> gene reduced plant height of both semidwarf and tall NILs. The non-lodging <i>SD1</i> + <i>Xa4</i> NILs of Pooja may suit in semi-deep-water ecology, whereas <i>sd1</i> + <i>Xa4</i> NILs are suitable for shallow lowlands.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-026-01633-0.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 2","pages":"16"},"PeriodicalIF":3.0,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12881224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Milyang54, a short, high-tillering winter wheat cultivar with enhanced lodging resistance and yield. 矮个、高分蘖、抗倒伏和高产的冬小麦品种米扬54。
IF 3 3区 农林科学
Molecular Breeding Pub Date : 2026-02-05 eCollection Date: 2026-02-01 DOI: 10.1007/s11032-026-01634-z
Hyeonjin Park, Jin-Kyung Cha, Youngho Kwon, So-Myeong Lee, Seung-Kyo Jeong, Ye Rin An, Woo-Jae Kim, Kyeong-Min Kim, Jong-Hee Lee
{"title":"Milyang54, a short, high-tillering winter wheat cultivar with enhanced lodging resistance and yield.","authors":"Hyeonjin Park, Jin-Kyung Cha, Youngho Kwon, So-Myeong Lee, Seung-Kyo Jeong, Ye Rin An, Woo-Jae Kim, Kyeong-Min Kim, Jong-Hee Lee","doi":"10.1007/s11032-026-01634-z","DOIUrl":"10.1007/s11032-026-01634-z","url":null,"abstract":"","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 2","pages":"15"},"PeriodicalIF":3.0,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12876502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combinations of mutations in the raffinose synthase genes and the fatty acid desaturase genes for improvement of soybean oil and meal traits. 棉子糖合酶基因与脂肪酸去饱和酶基因组合突变改善豆油和豆粕性状。
IF 3.6 3区 农林科学
Molecular Breeding Pub Date : 2026-01-23 eCollection Date: 2026-02-01 DOI: 10.1007/s11032-026-01636-x
Kristin Whitney, Militza Carrero-Colón, Senay Simsek, Karen Hudson
{"title":"Combinations of mutations in the raffinose synthase genes and the fatty acid desaturase genes for improvement of soybean oil and meal traits.","authors":"Kristin Whitney, Militza Carrero-Colón, Senay Simsek, Karen Hudson","doi":"10.1007/s11032-026-01636-x","DOIUrl":"10.1007/s11032-026-01636-x","url":null,"abstract":"<p><p>Soybean (<i>Glycine max</i>) meal and oil are important agricultural products, with soybean oil accounting for 55% of US vegetable oil consumption and soybean meal for 69% of global protein meal consumption. The ideal soybean oil profile for food applications is an oil high in the monounsaturated oleic acid, yet low in polyunsaturated fat which may result in instability and off-flavors. Soybean seeds also produce a limited amount of raffinose and stachyose, which are not digestible by non-ruminant animals, and limit the uses of soybean in meal and other food products. In recent years, efforts in soybean genetics have identified gene variants that can achieve the ideal oil profile and limit the biosynthesis of raffinose and stachyose in the seed. In this work conventional breeding techniques were employed to combine mutant alleles of genes for altered fatty acid and carbohydrate content, and the resulting lines were evaluated for additivity on aspects of seed composition. We found that we could obtain high oleic (> 75%) soybeans with < 2% of the seed mass comprising raffinose and stachyose using a combination of four mutant alleles. High oleic-low linolenic soybean with less than 3% linolenic acid, > 75% oleic acid and raffinose and stachyose < 2% of the seed weight were obtained by combining six genes. Composition changes were consistent over three growing seasons and no detrimental effects on overall seed protein or oil content were observed.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-026-01636-x.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 2","pages":"13"},"PeriodicalIF":3.6,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12830528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pyramiding blast resistance genes Pid3 and Pik-zh into a novel male sterile line Chuangyuan A for hybrid rice breeding. 将抗稻瘟病基因Pid3和Pik-zh导入杂交水稻创源a雄性不育系。
IF 3.6 3区 农林科学
Molecular Breeding Pub Date : 2026-01-16 eCollection Date: 2026-02-01 DOI: 10.1007/s11032-025-01629-2
Yihua Zhang, Shaojun Lin, Niqing He, Zhaoping Cheng, Shiyuan Fu, Ronghua Hu, Fenghuang Huang, Juguang Cai, Salah F Abou-Elwafa, Nora M Al Aboud, Dewei Yang
{"title":"Pyramiding blast resistance genes <i>Pid3</i> and <i>Pik-zh</i> into a novel male sterile line Chuangyuan A for hybrid rice breeding.","authors":"Yihua Zhang, Shaojun Lin, Niqing He, Zhaoping Cheng, Shiyuan Fu, Ronghua Hu, Fenghuang Huang, Juguang Cai, Salah F Abou-Elwafa, Nora M Al Aboud, Dewei Yang","doi":"10.1007/s11032-025-01629-2","DOIUrl":"10.1007/s11032-025-01629-2","url":null,"abstract":"<p><p>Rice blast is one of the most important diseases in rice production and poses a serious threat to rice production worldwide. Large-scale and long-term planting of rice varieties with a single resistance gene usually leads to losing resistance. Molecular marker-assisted selection is one of the most effective ways to control rice blast. In this study, the maintainer line 107B carrying <i>Pid3</i> and the maintainer line 253B carrying <i>Pik-zh</i> were used as donors, and individual strains of each generation were analyzed and identified by hybridization and marker-assisted selection (MAS). Through field planting and investigation, the fertility, main agronomic traits, combining ability, and heterosis of the Chuangyuan A were systematically analyzed. The results showed that in this study, a new three-line sterile line Chuangyuan A containing both <i>Pid3</i> and <i>Pik-zh</i> was cultivated, and the indoor and field indirect bacteria showed that the sterile line was resistant to blast. The sterile rate and sterility of Chuangyuan A were 100%, and the stigma exposure rate was 64.26%. Heterosis analysis showed that the yield of the combination prepared by Chuangyuan A was higher than that of the control Yiyou 673, which had good potential for production and application. In this study, Chuangyuan A with high blast resistance, stable sterility, high stigma exposure, and excellent agronomic and yield traits was developed by molecular marker-assisted breeding technique, which realized the directed improvement of blast resistance of the sterile line and created a new parent material for 3-line hybrid rice breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01629-2.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 2","pages":"10"},"PeriodicalIF":3.6,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12811192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fine mapping and cloning of an intersubspecific reproductive isolation locus MSHB1 in rice. 水稻亚种间生殖隔离位点MSHB1的精细定位与克隆。
IF 3.6 3区 农林科学
Molecular Breeding Pub Date : 2026-01-13 eCollection Date: 2026-01-01 DOI: 10.1007/s11032-025-01630-9
Xuan Wang, Zhengjiu Zhang, Jinfeng Zhao, Mengjiao Chen, Junyi Gong, Yu Han, Xin Wei, Xuehui Huang, Jiongjiong Fan
{"title":"Fine mapping and cloning of an intersubspecific reproductive isolation locus <i>MSHB1</i> in rice.","authors":"Xuan Wang, Zhengjiu Zhang, Jinfeng Zhao, Mengjiao Chen, Junyi Gong, Yu Han, Xin Wei, Xuehui Huang, Jiongjiong Fan","doi":"10.1007/s11032-025-01630-9","DOIUrl":"10.1007/s11032-025-01630-9","url":null,"abstract":"<p><p>Reproductive isolation between rice subspecies poses a significant barrier to harnessing heterosis in hybrid breeding. Here, we identified and characterized a novel locus, designated <i>Male Sterility-Related Hybrid Breakdown Locus on Chromosome 1</i> (<i>MSHB1</i>), through the utilization of chromosome segment substitution lines (CSSLs) derived from crosses between an <i>indica</i> cultivar Huanghuazhan (HHZ) and an <i>aromatic</i> cultivar Basmati Surkh 89 - 15 (BAS). A near-isogenic line, CS004, carrying the BAS-derived <i>MSHB1</i> <sup><i>BAS</i></sup> allele, exhibits complete male sterility, reduced plant height, fewer panicles, and shorter panicle length. Fine mapping localized <i>MSHB1</i> to a 28.583-kb interval on the short arm of chromosome 1. Genetic complementation confirmed that the <i>MSHB1</i> allele from HHZ is sufficient to restore fertility. <i>MSHB1</i> encodes a homolog of Esa1-associated factor 6 (EAF6), which regulates histone acetylation and transcriptional activity. Comparative genomic analyses revealed that functional divergence in promoter activity between duplicated <i>OsEAF6</i> paralogs (<i>MSHB1</i> and <i>MSHB12</i>) drives hybrid breakdown. These findings elucidate a molecular mechanism underlying intersubspecific reproductive isolation and highlight <i>MSHB1</i> as a strategic target for overcoming hybrid sterility to enhance heterosis in rice breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01630-9.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 1","pages":"9"},"PeriodicalIF":3.6,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12799846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145990095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrated transcriptome and translatome analyses reveal the early regulatory network of Brassica napus roots in response to the growth-promoting rhizobacterium Pseudomonas simiae WCS417. 整合转录组和翻译组分析揭示了甘蓝型油菜根对促生长的根杆菌Pseudomonas simae WCS417的早期调控网络。
IF 3 3区 农林科学
Molecular Breeding Pub Date : 2026-01-12 eCollection Date: 2026-01-01 DOI: 10.1007/s11032-025-01628-3
Li Zhang, Jie Liu, Zhipeng Zhou, Wei Wang
{"title":"Integrated transcriptome and translatome analyses reveal the early regulatory network of <i>Brassica napus</i> roots in response to the growth-promoting rhizobacterium <i>Pseudomonas simiae</i> WCS417.","authors":"Li Zhang, Jie Liu, Zhipeng Zhou, Wei Wang","doi":"10.1007/s11032-025-01628-3","DOIUrl":"https://doi.org/10.1007/s11032-025-01628-3","url":null,"abstract":"<p><p>Interactions between plant roots and complex microbial communities are critical for plant environmental adaptation. <i>Pseudomonas simiae</i> WCS417, a Gram-negative plant growth-promoting rhizobacterium (PGPR), is a model organism in plant-microbe interaction research and featured in over 750 studies since the 1990s. However, the translatome dynamics induced by WCS417 remain poorly understood. This study employed an integrated multi-omics approach, combining transcriptome (RNA-seq) and translatome (RNC-seq) analyses, to systematically investigate the transcriptional and translational regulatory networks in <i>Brassica napus</i> roots during early colonization by WCS417. Our results demonstrate that WCS417 significantly promotes lateral root formation, suppresses primary root elongation, and increases plant biomass. At the molecular level, WCS417 inoculation triggered extensive changes in gene expression and translation at 30 min and 6 h post-inoculation, affecting key processes including phytohormone signaling, cell wall remodeling, immune responses, and abiotic stress adaptation. Notably, although transcript levels of some immune-related genes were downregulated, their translation ratios was significantly enhanced, suggesting that plants maintain basal immunity while facilitating symbiotic establishment. Furthermore, WCS417 dynamically regulated genes involved in nitrogen/phosphorus uptake and core low-temperature response transcription factors in <i>Brassica napus</i> roots. These findings reveal a multi-layered regulatory mechanism by which WCS417 optimizes root system architecture and balances immunity with growth in <i>Brassica napus</i>, providing new insights into plant-microbe interactions.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01628-3.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 1","pages":"8"},"PeriodicalIF":3.0,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12796050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145970838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ludan510, an excellent maize variety with high yield and quality, disease-resistant and stress tolerance. 芦单510是高产优质、抗病耐寒的优良玉米品种。
IF 3 3区 农林科学
Molecular Breeding Pub Date : 2026-01-03 eCollection Date: 2026-01-01 DOI: 10.1007/s11032-025-01631-8
Zhaohua Ding, Wen Cheng, Hua Zhang, Zhiwu Wang, Yuan Tang, Zengbin Lu, Jianbing Yan, Yingjie Xiao
{"title":"Ludan510, an excellent maize variety with high yield and quality, disease-resistant and stress tolerance.","authors":"Zhaohua Ding, Wen Cheng, Hua Zhang, Zhiwu Wang, Yuan Tang, Zengbin Lu, Jianbing Yan, Yingjie Xiao","doi":"10.1007/s11032-025-01631-8","DOIUrl":"10.1007/s11032-025-01631-8","url":null,"abstract":"","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 1","pages":"6"},"PeriodicalIF":3.0,"publicationDate":"2026-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12764718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145906130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic exploration of β -glucan content in barley grains through GWAS and RNA-sequencing approaches. 利用GWAS和rna测序方法对大麦籽粒β -葡聚糖含量的遗传研究。
IF 3 3区 农林科学
Molecular Breeding Pub Date : 2026-01-03 eCollection Date: 2026-01-01 DOI: 10.1007/s11032-025-01625-6
Rizwan Ali Kumbhar, Kehan Yang, Tian Min, Sadaf Memon, Rania Chourouk Benhafid, Ji Jiale, Kashaf Mari, Yuan Cao, Weiwei Chen, Yajie Liu, Feng Zongyun, Hui Zhao
{"title":"Genetic exploration of <i>β</i> <i>-</i>glucan content in barley grains through GWAS and RNA-sequencing approaches.","authors":"Rizwan Ali Kumbhar, Kehan Yang, Tian Min, Sadaf Memon, Rania Chourouk Benhafid, Ji Jiale, Kashaf Mari, Yuan Cao, Weiwei Chen, Yajie Liu, Feng Zongyun, Hui Zhao","doi":"10.1007/s11032-025-01625-6","DOIUrl":"10.1007/s11032-025-01625-6","url":null,"abstract":"<p><p>Rapid advancements in molecular-level studies have highlighted significant changes and challenges in plant genetic studies. The current research on barley germplasm in China has shed light on the genetic mechanisms behind <i>β-</i>glucan content. In this study, the genotypic variation for 260 barley accessions was examined, with mean <i>β</i>-glucan values ranging from 3.22% to 5.48% across five environments. A genome-wide association study (GWAS) using a general linear model (GLM) identified 55 significant SNP markers on chromosomes 1 H, 3 H, 4 H, 5 H, 6 H, and 7 H, explaining 7.35% to 15.94% of the observed phenotypic variation. Transcriptome sequencing was performed on two contrasting germplasms, revealed 1,880 and 1,160 differentially expressed genes (DEGs) at 14 and 28 days post-anthesis, respectively, with pathways enriched in starch and sucrose metabolism, linking them to <i>β-</i>glucan variation. A new gene, <i>newGene_23124</i> (1 H), was identified with a functional annotation as a <i>beta</i>-D-glucan exohydrolase was identified as a strong candidate gene. GWAS results identified foundglycosyltransferase family protein genes, <i>HORVU.MOREX.r3.1HG0057970</i> (1 H), and <i>HORVU.MOREX.r3.3HG0279880</i> (3 H), which are involved in <i>β</i>-glucan synthesis and cell wall formation. Additionally, the genes <i>HORVU.MOREX.r3.4HG0337820</i> (4 H) and <i>HORVU.MOREX.r3.5HG0431360</i> (5 H), encoding for UDP-glycosyltransferase family proteins, were also identified. Furthermore, <i>HORVU.MOREX.r3.7HG0651610</i> (7 H), encoding a glycosyl hydrolase family 10 protein, was found to influence <i>β</i>-glucan content. In addition to these, several other genes were investigated. Overall, this research provides new insights into the genetic regulation of <i>β-</i>glucan content in barley and lays the foundation for breeding programs aimed at nutritional improvement of the crop.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-025-01625-6.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"46 1","pages":"7"},"PeriodicalIF":3.0,"publicationDate":"2026-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12764715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145905840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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