Cai Gao, Pengyan Wei, Zushu Xie, Pan Zhang, Muhammad Mobeen Tahir, Turgunbayev Kubanychbek Toktonazarovich, Yawen Shen, Xiya Zuo, Jiangping Mao, Dong Zhang, Yanrong Lv, Xiaoyun Zhang
{"title":"苹果NF-Y基因家族的基因组鉴定及参与开花转化的MdNF-YB18的功能分析。","authors":"Cai Gao, Pengyan Wei, Zushu Xie, Pan Zhang, Muhammad Mobeen Tahir, Turgunbayev Kubanychbek Toktonazarovich, Yawen Shen, Xiya Zuo, Jiangping Mao, Dong Zhang, Yanrong Lv, Xiaoyun Zhang","doi":"10.1007/s11032-024-01524-2","DOIUrl":null,"url":null,"abstract":"<p><p>Apple is a crucial economic product extensively cultivated worldwide. Its production and quality are closely related to the floral transition, which is regulated by intricate molecular and environmental factors. <i>Nuclear factor Y</i> (<i>NF-Y</i>) is a transcription factor that is involved in regulating plant growth and development, with certain <i>NF-Ys</i> play significant roles in regulating flowering. However, there is little information available regarding <i>NF-Ys</i> and their role in apple flowering development. In the present study, 51 NF-Y proteins were identified and classified into three subfamilies, including 11 MdNF-YAs, 26 MdNF-YBs, and 14 MdNF-YCs, according to their structural and phylogenetic features. Further functional analysis focused on <i>MdNF-YB18.</i> Overexpression of <i>MdNF-YB18</i> in <i>Arabidopsis</i> resulted in earlier flowering compared to the wild-type plants. Subcellular localization confirmed <i>MdNF-YB18</i> was located in the nuclear. Interaction between MdNFY-B18 and MdNF-YC3/7 was demonstrated through yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Yeast one-hybrid (Y1H) and the dual-luciferase reporter assays showed MdNF-YB18 could bind the promoter of <i>MdFT1</i> and activate its expression. Moreover, this activation was enhanced with the addition of MdNF-YC3 and MdNF-YC7. Additionally, MdNF-YB18 also could interact with <i>MdCOLs</i> (<i>CONSTANS Like</i>). This study lays the foundation for exploring the functional traits of MdNF-Y proteins, highlighting the crucial role of <i>MdNF-YB18</i> in activating <i>MdFT1</i> in <i>Malus</i>.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s11032-024-01524-2.</p>","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"45 1","pages":"4"},"PeriodicalIF":2.6000,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11668704/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genomic identification of the <i>NF-Y</i> gene family in apple and functional analysis of <i>MdNF-YB18</i> involved in flowering transition.\",\"authors\":\"Cai Gao, Pengyan Wei, Zushu Xie, Pan Zhang, Muhammad Mobeen Tahir, Turgunbayev Kubanychbek Toktonazarovich, Yawen Shen, Xiya Zuo, Jiangping Mao, Dong Zhang, Yanrong Lv, Xiaoyun Zhang\",\"doi\":\"10.1007/s11032-024-01524-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Apple is a crucial economic product extensively cultivated worldwide. Its production and quality are closely related to the floral transition, which is regulated by intricate molecular and environmental factors. <i>Nuclear factor Y</i> (<i>NF-Y</i>) is a transcription factor that is involved in regulating plant growth and development, with certain <i>NF-Ys</i> play significant roles in regulating flowering. However, there is little information available regarding <i>NF-Ys</i> and their role in apple flowering development. In the present study, 51 NF-Y proteins were identified and classified into three subfamilies, including 11 MdNF-YAs, 26 MdNF-YBs, and 14 MdNF-YCs, according to their structural and phylogenetic features. Further functional analysis focused on <i>MdNF-YB18.</i> Overexpression of <i>MdNF-YB18</i> in <i>Arabidopsis</i> resulted in earlier flowering compared to the wild-type plants. Subcellular localization confirmed <i>MdNF-YB18</i> was located in the nuclear. 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Genomic identification of the NF-Y gene family in apple and functional analysis of MdNF-YB18 involved in flowering transition.
Apple is a crucial economic product extensively cultivated worldwide. Its production and quality are closely related to the floral transition, which is regulated by intricate molecular and environmental factors. Nuclear factor Y (NF-Y) is a transcription factor that is involved in regulating plant growth and development, with certain NF-Ys play significant roles in regulating flowering. However, there is little information available regarding NF-Ys and their role in apple flowering development. In the present study, 51 NF-Y proteins were identified and classified into three subfamilies, including 11 MdNF-YAs, 26 MdNF-YBs, and 14 MdNF-YCs, according to their structural and phylogenetic features. Further functional analysis focused on MdNF-YB18. Overexpression of MdNF-YB18 in Arabidopsis resulted in earlier flowering compared to the wild-type plants. Subcellular localization confirmed MdNF-YB18 was located in the nuclear. Interaction between MdNFY-B18 and MdNF-YC3/7 was demonstrated through yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Yeast one-hybrid (Y1H) and the dual-luciferase reporter assays showed MdNF-YB18 could bind the promoter of MdFT1 and activate its expression. Moreover, this activation was enhanced with the addition of MdNF-YC3 and MdNF-YC7. Additionally, MdNF-YB18 also could interact with MdCOLs (CONSTANS Like). This study lays the foundation for exploring the functional traits of MdNF-Y proteins, highlighting the crucial role of MdNF-YB18 in activating MdFT1 in Malus.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01524-2.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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