Molecular Imaging and Biology最新文献

{"title":"One-step Radiosynthesis and Preclinical Evaluation of Molecular Tracer [¹⁸F]FEtO-CHC Targeting Monocarboxylate Transporters for PET Imaging in Tumor-bearing Mice.","authors":"Dongmei Shi, Ling Liu, Di Zhang, Yuzhou Zheng, Wenhao Hu, Ping Wu, Xinzhong Hao, Haiyan Liu, Jie Gao, Jianguo Li, Zhifang Wu, Sijin Li, Hongliang Wang","doi":"10.1007/s11307-025-02024-1","DOIUrl":"https://doi.org/10.1007/s11307-025-02024-1","url":null,"abstract":"<p><strong>Purpose: </strong>Monocarboxylate transporters (MCTs) play a pivotal role in tumor metabolic symbiosis, acid resistance, and metastatic progression. Herein, we report the development of [¹⁸F]FEtO-CHC, a novel MCTs-targeted positron emission tomography (PET) radiotracer, and systematically evaluate its potential for non-invasive tumor imaging.</p><p><strong>Procedures: </strong>The radiosynthesis of [¹⁸F]FEtO-CHC and its non-radioactive analog was achieved through optimized precursor synthesis and fluorination protocols. Comprehensive in vitro characterization encompassed: radiochemical purity and stability assessments, cellular uptake kinetics and inhibition assays in MCT-expressing BxPC3 (pancreatic) and 4T1 (breast) cancer models, biodistribution and dynamic micro-PET/CT imaging in tumor-bearing murine models.</p><p><strong>Results: </strong>[¹⁸F]FEtO-CHC, a CHC-derived radioligand, was synthesized via streamlined one-step radiosynthesis with 52.08 ± 6.74% decay-corrected yield (n=7), >99% radiochemical purity, and excellent stability. Cellular studies demonstrated MCTs-dependent uptake with significant suppression (>70%) by α-CHC competition. In vivo pharmacokinetics revealed favorable metabolic stability with dual hepatorenal clearance. Tumor uptake correlated with MCT expression levels, as confirmed by immunohistochemistry.</p><p><strong>Conclusions: </strong>This study establishes an efficient one-step radiosynthetic approach for [¹⁸F]FEtO-CHC production and validates its specificity as a MCT-targeted PET probe, offering potential utility in tumor imaging with further structural optimization.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term Local Control Following CEA-targeted Fluorescence-guided Surgery in Patients With Locally Advanced and Recurrent Rectal Cancer.","authors":"Mats I Warmerdam, Davy M J Creemers, Miranda Kusters, Koen C M J Peeters, Fabian A Holman, J Sven D Mieog, Francoise Cailler, Pim J W A Burger, Jacobus Burggraaf, Harm J T Rutten, Cornelis Verhoef, Alexander L Vahrmeijer, Denise E Hilling","doi":"10.1007/s11307-025-02021-4","DOIUrl":"https://doi.org/10.1007/s11307-025-02021-4","url":null,"abstract":"<p><strong>Purpose: </strong>In our previous phase 2 trial, patients with locally advanced (LARC) or locally recurrent rectal cancer (LRRC) received SGM-101, a CEA-targeted fluorescent agent, to enable real-time near-infrared fluorescence (NIRF) guided surgery. This study demonstrated that SGM-101 enabled additional tumor removal in some patients and supported less invasive surgery in others. Despite this positive intraoperative effect, the impact on long-term tumor control is unknown. Therefore, in this article we report the long-term outcomes of all rectal cancer patients that participated to the trial.</p><p><strong>Procedures: </strong>For all 29 LARC and LRRC patients that participated in the SGM-101 phase 2 trial, follow-up data were collected. Main outcome measure was 5-year local tumor control.</p><p><strong>Results: </strong>The median follow-up of all patients was 5.0 years (IQR 4.5-5.5). Of the 12 LARC patients, three (25%) patients developed a local recurrence. The two patients in whom NIRF-guided surgery resulted in less invasive surgery remained locally recurrence-free. Among the 17 patients undergoing curative surgery for LRRC, 11 (65%) patients developed a local re-recurrence. Of the three patients who had an R0 instead of R1 as a direct result of SGM-101 guided surgery, one patient developed a local re-recurrence (33%), while the other two remained local recurrence-free.</p><p><strong>Conclusions: </strong>This is the first study to report follow-up data on patients undergoing tumor-targeted NIRF-guided surgery. Although SGM-101 resulted in warranted changes in surgical management intra-operatively, no improved long-term benefit could be observed for the entire cohort. However, the subset of patients whose surgical approach was modified based on NIRF - either by performing less invasive surgery or removing additional malignant tissue-showed favorable long-term outcomes. Results from ongoing large trials are awaited.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of ¹⁷⁷Lu-Labeled Lipiodol as a Targeted Radionuclide Therapy for Hepatocellular Carcinoma in a Preclinical Xenograft Model.","authors":"Yumiko Kono, Keita Utsunomiya, Takahiro Shiraishi, Naoki Kan, Ichiro Shiojima, Kaoru Maruyama, Noboru Tanigawa","doi":"10.1007/s11307-025-02016-1","DOIUrl":"https://doi.org/10.1007/s11307-025-02016-1","url":null,"abstract":"<p><strong>Background: </strong>Lutetium-177 (¹⁷⁷Lu) is a promising radionuclide for targeted cancer therapy due to its favorable theranostic properties. Transarterial lipiodol embolization is widely used for hepatocellular carcinoma (HCC), but the potential of ¹⁷⁷Lu emulsified into lipiodol (¹⁷⁷Lu-lipiodol) remains underexplored. This study aimed to evaluate the partition coefficient, biodistribution, and antitumor efficacy of ¹⁷⁷Lu-lipiodol in a preclinical xenograft model.</p><p><strong>Methods: </strong>After synthesizing ¹⁷⁷Lu-oxine from ¹⁷⁷Lu-chloride, the product was emulsified in lipiodol. Its radiochemical purity and partition coefficient were measured. F344 NJcl rnu/nu rats (n = 5) bearing bilateral thigh tumors (HC-4 cells) were randomized to receive ¹⁷⁷Lu-lipiodol (2.8 MBq in 50 μL) or non-labeled lipiodol (50 μL) via surgical exposure and direct puncture of the right femoral artery. SPECT/CT images were acquired over 14 days, and biodistribution was confirmed by gamma counting at day 28. Tumor volumes and body weights were monitored to assess treatment response and toxicity.</p><p><strong>Results: </strong>The ¹⁷⁷Lu-lipiodol emulsion was obtained with a high radiochemical purity (> 99%). SPECT/CT showed high tumor accumulation (34.0% ± 4.4% immediately post-injection) that persisted up to day 28 (7.3% ± 1.2% of injected dose). Tumor growth was significantly suppressed with a treated-to-untreated volume ratio of 0.45 at day 14 (p = 0.017) and 0.59 at day 21 (p = 0.001). While off-target uptake was limited, moderate splenic accumulation (26.6% ± 17.5% ID) was noted. No marked body weight changes or gross organ abnormalities were observed.</p><p><strong>Conclusion: </strong>¹⁷⁷Lu-lipiodol for HCC therapy demonstrated effective tumor targeting and growth suppression of HCC in a preclinical xenograft model.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Go Fish! Hepatic Uptake of Clinical Hepatospecific Gadolinium-Based MRI Contrast Agents in Zebrafish is Similar to Humans.","authors":"Josie A Shapiro, Tapas Bhattacharyya, Lauren A Squire, Christiane L Mallett, Jeremy M-L Hix, Legend E Kenney, Aitor Aguirre, Erik M Shapiro","doi":"10.1007/s11307-025-02023-2","DOIUrl":"https://doi.org/10.1007/s11307-025-02023-2","url":null,"abstract":"<p><strong>Purpose: </strong>Zebrafish are a useful organism for investigating liver disease due to their genetic similarities with humans, particularly in genes associated with liver function. It has been posited that liver function can be assessed non-invasively by MRI, measuring the hepatic accumulation of gadolinium-based contrast agents (GBCAs). We characterized the hepatic uptake of various hepatospecific and non-hepatospecific clinical GBCAs in zebrafish.</p><p><strong>Procedures: </strong>To introduce GBCAs systemically, zebrafish swam for 30 min in water containing 5 mM of various clinical hepatospecific or non-hepatospecific GBCAs. Fish were then sacrificed and underwent 3D, T1-weighted, high-resolution MRI at 9.4 T. In vitro MRI and transport studies of the same GBCAs were conducted in HEK293T cells transiently expressing OATP1D1, OATP1B2 and OATP1B3.</p><p><strong>Results: </strong>T1-weighted ex-vivo MRI of zebrafish showed hyperintensity in the liver, gall bladder, bile ducts, and intestine for fish swimming in gadoxetate, but not for in gadobenate nor gadoterate. In vitro cell experiments confirm that gadoxetate but not gadobenate is efficiently transported by OATP1D1.</p><p><strong>Conclusion: </strong>Zebrafish liver accumulates gadoxetate but not gadobenate via OATP1D1 transport, among the two clinical hepatospecific MRI GBCAs, and also does not accumulate gadoterate, a non-hepatospecific GBCA. This pattern of GBCA hepatic uptake is similar to humans but differs from all other non-primates reported, which exhibit high hepatic uptake of both gadoxetate and gadobenate.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Background Tissue with Native Target Expression Can Determine Presence of Nodal Metastasis in Head and Neck Squamous Cell Carcinoma Patients Infused with Targeted Fluorescent Tracers.","authors":"Nicole Meeks, Sherin James, Giri Krishnan, Akhilesh Wodeyar, Hidenori Tanaka, Benjamin B Kasten, Yu-Jin Lee, Marisa E Hom, Eben L Rosenthal, Jason M Warram","doi":"10.1007/s11307-025-01996-4","DOIUrl":"10.1007/s11307-025-01996-4","url":null,"abstract":"<p><strong>Purpose: </strong>Survival and treatment intensity in patients with head and neck squamous cell carcinoma (HNSCC) is determined by the presence of lymph node (LN) metastasis, and as a result surgical removal of potentially affected LN remains a mainstay practice. Fluorescence guided surgery (FGS) using targeted optical agents is an expanding field that shows great potential for aiding diagnosis of metastatic LN. Given variations in fluorescence background, a reference standard for regions of interest is necessary for cross patient comparison. The present study aims to determine whether tissue with native target expression can be used as a background to determine metastatic LN in patients with HNSCC infused with anti-epidermal growth factor receptor (EGFR) targeted imaging agents.</p><p><strong>Procedures: </strong>Twenty-two patients infused with panitumumab-IRDye800 or cetuximab-IRDye800 prior to surgery were included. Fluorescence imaging and analysis was performed on resected LNs (N = 843) using the submandibular glands (SMG) and skin as reference standard tissue with known EGFR antigen expression.</p><p><strong>Results: </strong>Sixteen patients (72.7%) had at least one positive LN on final pathology. The LN to SMG (LN/SMG) and LN to skin (LN/skin) ratios were significantly higher in metastatic LN compared to benign LN (p < 0.0001 for both). Using patient-specific ratios to determine an optimal LN/skin cutoff was the most sensitive (95.2%) and directly comparing the LN/skin ratio of all patients to determine a cutoff was the most specific (86.3%).</p><p><strong>Conclusions: </strong>In HNSCC patients infused with a molecularly targeted fluorescent tracer, endogenous expression of the target antigen can be used as a reference standard to detect LN metastasis. Additionally, the performance of the background in determining metastatic LN can be improved by utilizing patient-specific reference standards.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"333-340"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species-Specific Hepatic Uptake of [⁶⁴Cu]Cu-EOB-NOTA, A Newly Designed Hepatospecific PET Agent.","authors":"Jinda Fan, Bijja Janaki Ramulu, Christiane L Mallett, Legend E Kenney, Nathan Kauffman, Tapas Bhattacharyya, Maryam Sabbaghan, Satyendra Singh, Kurt R Zinn, Erik M Shapiro","doi":"10.1007/s11307-025-02009-0","DOIUrl":"10.1007/s11307-025-02009-0","url":null,"abstract":"<p><strong>Purpose: </strong>Measuring hepatic flux rates of transportable substrates has the potential for assessing liver function. PET imaging of a PET-enabled substrate may provide a more straightforward measurement of time-dependent substrate concentration through the liver than MRI using an MRI contrast agent. Here we synthesized and evaluated the hepatobiliary transport of a new hepatospecific PET agent designed for stable Cu²⁺ chelation and transport by hepatic OATPs, [⁶⁴Cu]Cu-EOB-NOTA.</p><p><strong>Procedures: </strong>EOB-NOTA was synthesized, its two enantiomers separated by chiral HPLC, and individually radiolabeled with [⁶⁴Cu]Cu²⁺. Cocktails of each enantiomer of [⁶⁴Cu]Cu-EOB-NOTA and Gd-EOB-DTPA were formulated for simultaneous PET/MRI imaging of hepatic flux by PET and MRI. Two mouse models were evaluated: wild-type mice and mice expressing only human hepatic OATPs.</p><p><strong>Results: </strong>In wild-type mice, [⁶⁴Cu]Cu-EOB-NOTA hepatic influx and efflux was high, but slower compared to Gd-EOB-DTPA. Neither enantiomer of [⁶⁴Cu]Cu-EOB-NOTA exhibited detectable transport into the liver in mice expressing human OATPs. This was validated by waste clearance studies and in vitro uptake assays in cells engineered to express rodent and human OATPs.</p><p><strong>Conclusion: </strong>[⁶⁴Cu]Cu-EOB-NOTA exhibited no detectable hepatic uptake by transgenic mice expressing human hepatic transporters. This finding was surprising given the efficient transport of the structurally similar metal chelate Gd-EOB-DTPA, and underscores challenges in the design of imaging molecular probes, including poor predictability for hepatic transport, and the value of validating new agents in mice expressing human hepatic transporters.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"305-312"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[¹⁸F]NaF PET/CT Imaging of Iliac Bones to Assess Bone Turnover.","authors":"Shashi B Singh, Om H Gandhi, Bimash B Shrestha, Patrick Glennan, Anuradha Rosario Bahadur, Niloofaralsadat Motamedi, Kishor Khanal, Sagar Wagle, Poul Flemming Høilund-Carlsen, Thomas J Werner, Mona-Elisabeth Revheim, Abass Alavi","doi":"10.1007/s11307-025-02003-6","DOIUrl":"10.1007/s11307-025-02003-6","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigated the effects of laterality, age, gender, BMI, and physical activity level on iliac bone turnover using [¹⁸F]NaF PET/CT.</p><p><strong>Procedures: </strong>Fifty-nine males and 44 females from the CAMONA study were analyzed. A region of interest (ROI) was drawn to segment the iliac bone using Hounsfield unit thresholds and morphological closing algorithm. [¹⁸F]NaF SUVmean was compared between the left and right iliac bones using a paired t-test, while Pearson correlation coefficient assessed changes with age, BMI, and physical activity level.</p><p><strong>Results: </strong>[¹⁸F]NaF uptake was higher in right iliac bone than left in males, females, and the combined-group. In males, SUVmean was 2.98 ± 1.63 (1.1-7.87) on left and 3.71 ± 1.49 (1.49-3.7) on right. In females, SUVmean was 2.59 ± 1.14 (0.88-6.27) on left and 3.72 ± 1.04 (2.22-6.51) on right. Combined, SUVmean was 2.81 ± 1.44 (0.88-7.87) on left and 3.71 ± 1.31 (0.89-8.07) on right. [¹⁸F]NaF uptake negatively correlated with age (right: r = - 0.27, P = 0.006; left: r = - 0.22, P = 0.02), stronger in females (right: r = - 0.30, P = 0.04; left: r = - 0.31, P = 0.04) than males (right: r = - 0.26, P = 0.04; left: r = - 0.18, P = 0.18). SUVmean correlated positively with BMI in males (right: r = 0.47, P = 0.0002; left: r = 0.38, P = 0.0027), females (right: r = 0.36, P = 0.0168; left: r = 0.30, P = 0.0505), and combined-group (right: r = 0.43, P < 0.0001; left: r = 0.37, P = 0.0001). No significant correlation was found between SUVmean and physical activity in males, while in females, a negative correlation was observed on left (r = - 0.37, P = 0.0390) but not on right (r = - 0.27, P = 0.1302), and when combined, the correlation remained significant on left (r = - 0.24, P = 0.0372) but not on right (r = - 0.16, P = 0.1541).</p><p><strong>Conclusions: </strong>[¹⁸F]NaF uptake was higher in the right iliac bone and declined with age, particularly in females. The positive correlation between SUVmean and BMI; and the negative correlation between SUVmean and physical activity suggest metabolic influences on bone turnover. [¹⁸F]NaF PET/CT may serve as a tool for assessing bone metabolism and turnover in asymptomatic individuals.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"295-304"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Preclinical Development of Near-Infrared-Labeled CD38-Targeted Daratumumab for Optical Imaging of CD38 in Multiple Myeloma.","authors":"Nicholas Cho, Sooah Ko, Monica Shokeen","doi":"10.1007/s11307-025-01984-8","DOIUrl":"10.1007/s11307-025-01984-8","url":null,"abstract":"","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"495-497"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Neoadjuvant Therapies Do Not Reduce Epidermal Growth Factor Receptor (EGFR) Expression or EGFR-Targeted Fluorescence in a Murine Model of Soft-Tissue Sarcomas.","authors":"Samuel S Streeter, Xiaochun Xu, Kendra A Hebert, Paul M Werth, P Jack Hoopes, Lesley A Jarvis, Brian W Pogue, Keith D Paulsen, Kimberley S Samkoe, Eric R Henderson","doi":"10.1007/s11307-025-01999-1","DOIUrl":"10.1007/s11307-025-01999-1","url":null,"abstract":"","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"498"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Heat Stroke-Induced Brain Injury: A Preclinical Study with a Rat Model Using ¹⁸F-FDG Brain PET.","authors":"Daehee Kim, Hye Won Lee, Byung Seok Moon, Sun Mi Park, Ji Eun Lee, Bom Sahn Kim, Woon Jeong Lee, Hai-Jeon Yoon","doi":"10.1007/s11307-025-02008-1","DOIUrl":"10.1007/s11307-025-02008-1","url":null,"abstract":"<p><strong>Purpose: </strong>Heat stroke is the most serious heat-related illness and is recognized as a worldwide public concern as global temperatures continue to rise. Although the clinical neurological complications of heat stroke are relatively well described, a limited number of studies exist that document imaging findings. Therefore, in this preclinical study, we aimed to identify the imaging findings of ¹⁸F-FDG brain PET following heat stroke and elucidate the utility of FDG PET in the evaluation of heat stroke-induced brain injury.</p><p><strong>Methods: </strong>Heat stroke was induced in Sprague Dawley rats by placing them in a hot and humid chamber maintained without food and water until they exhibited the heat stroke onset diagnostic criterion. Three hours after the induction ended, ¹⁸F-FDG brain PET images were acquired in 7 controls and 14 rats with heat stroke. Between groups, region-based (standardized uptake values were normalized to the whole brain and SUV of the whole brain (SUV_WB), and voxel-based analyses were performed.</p><p><strong>Results: </strong>Of the 14 rats with heat stroke, 8 survived, whereas 6 did not. In the region-based and voxel-base analyses, the rats that did not survive showed significantly higher SUVR_HB in the hypothalamus and significantly lower SUVR_HB in several cortical regions than the controls as well as the survived rats. In the region-based analysis, the survived rats showed a significant increase or decrease in SUVR_HB compared to the controls in a few cortical regions. However, no difference was observed in the voxel-based analysis.</p><p><strong>Conclusions: </strong>The 3-h post-injury PET scan showed a distinctly different regional distribution of ¹⁸F-FDG in the brains of lethally injured heat stroke rats compared to the controls as well as the survived rats. The ¹⁸F-FDG brain PET may have the potential to provide early indicators of catastrophic injury and reflect the early neurological pathophysiology of heat stroke.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"442-453"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}