Molecular Imaging and Biology最新文献

{"title":"Comparison of the Correlation Between Cerebral [¹⁸F]FDG Metabolism as Assessed by Two Asymmetry Indices and Clinical Neurological Score in Patients with Ischemic Cerebrovascular Disease.","authors":"Yuxin Liang, Bixiao Cui, Linlin Ye, Bin Yang, Yi Shan, Hongwei Yang, Lei Ma, Miao Zhang, Jie Lu","doi":"10.1007/s11307-025-02002-7","DOIUrl":"10.1007/s11307-025-02002-7","url":null,"abstract":"<p><strong>Purpose: </strong>Two types of asymmetry index (AI) have been utilized in evaluating cerebral function in ischemic cerebrovascular disease, however, few data exist on the differences between these AI measures. This study aimed to compare the two AIs in assessing PET cerebral metabolism and their correlation with clinical scales, to explore their potential value and applications in clinical settings.</p><p><strong>Procedures: </strong>Seventy patients diagnosed with subacute and chronic ischemic stroke were retrospectively analyzed. All patients underwent 2-deoxy- 2-[¹⁸F]fluoro-D-glucose ([¹⁸F]FDG) PET/MR scans and were assessed using the National Institutes of Health Stroke Scale (NIHSS) and the Modified Rankin Scale (mRS). Nineteen patients underwent a repeat [¹⁸F]FDG PET/MR scan one year later. Two voxel-wise AI methods, designated as AI₁ and AI₂, were calculated based on standardized uptake value ratio (SUVR). The hypometabolism on affected side assessed by different AI methods were compared. The correlations between the hypometabolism and the clinical scores were analyzed.</p><p><strong>Results: </strong>The volume and percentage of decreased [¹⁸F]FDG metabolism assessed by AI₂ was larger than that obtained from AI₁ (all p < 0.0001). The correlation coefficients between the clinical scores and the decreased metabolism in temporal and parietal lobes assessed by AI₁ method were all higher than those from AI₂. In addition, the improved follow-up patients showed more pronounced metabolic improvement as assessed by AI₁.</p><p><strong>Conclusions: </strong>The assessment of cerebral [¹⁸F]FDG metabolism in patients with unilateral internal carotid/middle cerebral artery steno-occlusion to reflect clinical neurological function using the AI₁ method demonstrated superior performance in comparison to the AI₂ method.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"454-464"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Mechanical Ventilation and Anesthesia on PET Tracer Kinetics for Combined PET/fMRI Studies in Rats.","authors":"Yan Ma, Laura Kuebler, Sabrina Haas, Andreas Maurer, Kristina Herfert","doi":"10.1007/s11307-025-02006-3","DOIUrl":"10.1007/s11307-025-02006-3","url":null,"abstract":"<p><strong>Purpose: </strong>Positron Emission Tomography (PET), a crucial tool in molecular brain imaging, has evolved into a hybrid system through integration with functional MRI (fMRI). This advancement facilitates the simultaneous recording of molecular and functional data in animal models, offering insights into neuroreceptor and neurotransmitter dynamics and their effects on brain function. While mechanical ventilation is often used in small animal fMRI to stabilize physiological blood gas levels, its effects on PET tracer kinetics remain underexplored.</p><p><strong>Procedures: </strong>This study examines the kinetics of [¹¹C]raclopride, a dopamine-sensitive PET tracer targeting D2/D3 receptors, under various respiratory conditions and anesthesia protocols frequently used in small animal fMRI and PET.</p><p><strong>Results: </strong>Results indicate significant variations in tracer kinetics: increased peak levels, a shorter time to peak, and a faster tracer equilibrium in standard uptake value ratio were observed in spontaneously breathing animals versus those under mechanical ventilation. The anesthesia type also strongly influenced the tracer kinetics: α-chloralose anesthesia reduced brain uptake, whereas isoflurane led to a more rapid equilibrium.</p><p><strong>Conclusions: </strong>These findings underscore the profound impact of mechanical ventilation and anesthesia selection on PET tracer kinetics in hybrid PET/fMRI studies. The study highlights that those protocols established for fMRI are not directly transferable to PET imaging in small animals, emphasizing the necessity for a careful investigation of the influence of anesthesia and ventilation techniques on tracer kinetics.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"313-323"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[18 F]-Fluoroestradiol PET (FES-PET) and [18 F] Flurodeoxyglucose PET (FDG-PET) Imaging May Aid in Managing Therapy in Patients with Metastatic Lobular Breast Cancer.","authors":"Poorni M Manohar, Lanell M Peterson, Isaac C Jenkins, Qian Vicky Wu, Brenda F Kurland, Alena Novakova-Jiresova, Mark Muzi, Delphine L Chen, Jennifer M Specht, Suzanne Dintzis, Paul E Kinahan, David A Mankoff, Hannah M Linden","doi":"10.1007/s11307-025-02015-2","DOIUrl":"10.1007/s11307-025-02015-2","url":null,"abstract":"<p><strong>Aim: </strong>This study examines the combination of FES-PET and FDG-PET as complementary imaging for detection of metastatic ILC.</p><p><strong>Methods: </strong>We retrospectively evaluated FES and FDG uptake in patients with metastatic ILC from an estrogen receptor (ER) positive primary tumor. We classified lesions into three categories (FES high/FDG low, FES high/FDG high, FES low/FDG low) using SUVmax cut-off values of 1.5 for FES and 5.0 for FDG. Qualitative evaluation included examination of the difference in the extent of disease between FES and FDG.</p><p><strong>Results: </strong>Of the 38 patients, 82% had FES uptake in all tumor sites identified by FDG, with 18% lacking FES uptake in at least one lesion. Mean (range) SUVmax for FES and FDG was 4.0 (0.67-10.6) and 4.6 (1.3-12.5), respectively. The majority of ILC patients (25/38), had lesions with FES high/FDG low uptake, consistent with the strongly ER + indolent nature of ILC. Patients with disease classified as FES high/FDG low had longer median overall survival (OS) (3.2 years) and progression-free survival (PFS) (1.5 years) than FES high/FDG high (OS = 2.1 years and PFS = 0.46 years). The median overall OS for all patients was 3.0 years (95% CI 2.5, 4.8) and PFS of 1.3 years (95% CI 0.6, 2.5). 8 patients (21%) had qualitatively more extensive disease by FES-PET.</p><p><strong>Conclusions: </strong>Our findings suggest that both FES-PET and FDG-PET can identify metastatic ILC and be useful in detecting the pattern and extent of disease. The imaging combination provides additional information for prognosis and clinical decision making, balancing suitability for endocrine therapy and aggressiveness/indolence of disease.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"410-420"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attenuation Estimation and Acoustic Characterization of Mouse Lymph Node Tumor Using High-frequency Ultrasound.","authors":"Masaaki Omura, Kazuki Maeda, Kazuki Tamura, Kenji Yoshida, Ariunbuyan Sukhbaatar, Tetsuya Kodama, Tadashi Yamaguchi","doi":"10.1007/s11307-025-02007-2","DOIUrl":"10.1007/s11307-025-02007-2","url":null,"abstract":"<p><strong>Purpose: </strong>Lymph node (LN) biopsy is the gold standard for diagnosing metastasis. While ultrasound imaging is a non-invasive method for real-time LN metastasis diagnosis and tumor assessment, its accuracy depends on operator skill and system settings. Quantitative ultrasound can characterize tissue microstructure changes due to tumors, offering operator-independent parameters, and one of the quantitative ultrasound methods, the backscatter coefficient, is necessary to compensate for tissue attenuation. However, the change in the attenuation coefficient (AC) in the tumor growth is uncertain. Using in vivo high-frequency ultrasound (25 MHz) measurement and scanning acoustic microscopy (80 and 300 MHz) for ex vivo samples, we aim to investigate how tumor growth is linked to the attenuation and acoustic properties such as acoustic impedance and speed of sound related to ultrasonic wave propagation.</p><p><strong>Procedures: </strong>FM3 A-Luc mammary carcinoma cells were inoculated into the subiliac LNs of mice, and tumor progression was monitored over time. Bioluminescence imaging was used to assess tumor growth, while ultrasound measurements focused on estimating AC and other acoustic properties.</p><p><strong>Results: </strong>Results indicated that the mean of AC decreased, and its standard deviation increased as tumors grew, correlating with bioluminescence intensity. Furthermore, acoustic impedance and speed of sound varied between normal and tumor tissues, revealing differences in tissue microstructure from the histopathological images.</p><p><strong>Conclusions: </strong>The finding of a decrease in AC observed with tumor growth may play a crucial role in enhancing the accuracy of quantitative ultrasound on attenuation compensation, potentially improving the differentiation between metastatic and non-metastatic LNs.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"379-388"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Construction of I-124 Labeled Small Molecular Probe for Noninvasive PET Imaging of CAIX Expression.","authors":"Xianteng Yang, Chengxue He, Feng Wang, Li Wen, Haifeng Huang, Jing Wang, Zhi Yang, Hua Zhu","doi":"10.1007/s11307-025-02004-5","DOIUrl":"10.1007/s11307-025-02004-5","url":null,"abstract":"<p><strong>Purpose: </strong>Carbonic anhydrase IX (CAIX) which is high expression in the most of hypoxic tumor than normal tissue, promoting the growth, invasion, and metastasis of the tumor. Therefore, the study aimed to evaluate the retention and diagnostic ability of [¹²⁴I]I-XYIMSR- 01 in CAIX-overexpression tumor by using positron emission tomography (PET) imaging.</p><p><strong>Procedures: </strong>[^124/125I]I-XYIMSR- 01 was labeled by ^124/125I, and its CAIX-targeting properties in different cell lines were assayed by cell uptaken study. Its diagnose and retention ability in vivo were verified in different CAIX-expression models using PET imaging and biodistribution study. Pathological tissues were obtained for immunohistochemical (IHC) and Hematoxylin-Eosin (HE) staining to explore the relationship between CAIX and hypoxic, and further analyze PET/CT results.</p><p><strong>Results: </strong>[¹²⁴I]I-XYIMSR- 01 was obtained with high specific activity, good radiochemical purity, and good stability. The uptake of of [¹²⁴I]I-XYIMSR- 01 in HT- 29 cells, which have high CAIX expression, was significant higher than that in HCT116 cells with low CAIX expression (12.78 ± 0.47 vs 1.06 ± 0.10, p = 0.000, at 1 h). This indicated that the probe has good targeting capability and specificity for CAIX. In Micro-PET imaging, clear molecular images lasting for 48 h were achieved in HT29 model. Quantitative biodistribution results showed that the tumor and digestive tract background tissues had a good signal-to-noise ratio within 24 h after injection, indicating [¹²⁴I]I-XYIMSR- 01 could enable delayed imaging in digestive tract tumors (tumor-to-small intestine: 8.79 ± 0.98). Tumors uptakes were also confirmed by IHC pathology.</p><p><strong>Conclusion: </strong>The study have shown that [¹²⁴I]I-XYIMSR- 01 is an ideal molecular probe for tumor hypoxia, enabling long-term dynamic monitoring and imaging of hypoxic tumors.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"485-494"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Imaging Value of ¹⁸F-FAPI PET/CT in Sorafenib-Induced Cardiac Dysfunction in Patients with Hepatocellular Carcinoma: Compared with ¹⁸F-FDG PET/CT.","authors":"Yingqi Luo, Qingqi Yang, Xiaowen Qin, Boyang Yu, Shengnan Jiang, Ying Liu","doi":"10.1007/s11307-025-02005-4","DOIUrl":"10.1007/s11307-025-02005-4","url":null,"abstract":"<p><strong>Purpose: </strong>Evaluation of ¹⁸F-FAPI PET/CT imaging in sorafenib-induced cardiac dysfunction in hepatocellular carcinoma (HCC) patients, and compared with ¹⁸F-FDG PET/CT.</p><p><strong>Procedures: </strong>This retrospective study enrolled 75 HCC patients treated with sorafenib at our institution from June 2021 to June 2023. All patients underwent ¹⁸F-FDG PET/CT six months after treatment, followed by ¹⁸F-FAPI PET/CT within the subsequent week. Patients were divided into cardiac dysfunction group and control group based on the definition of cancer therapy-related cardiac dysfunction (CTRCD). Myocardial uptake parameters on ¹⁸F-FDG and ¹⁸F-FAPI PET/CT were compared between the two groups. The primary endpoint was the occurrence of major adverse cardiac events (MACEs), and the secondary endpoint was all-cause mortality, with follow-up at 30, 90, and 180 days after the PET/CT examinations.</p><p><strong>Results: </strong>This study ultimately enrolled 47 patients, with the cardiac dysfunction group (n = 9) and control group (n = 38) demonstrating significant differences in myocardial ¹⁸F-FAPI high uptake, left ventricular (LV) ¹⁸F-FDG SUV, LV/liver ¹⁸F-FDG SUV, myocardial ¹⁸F-FAPI SUV, myocardial/aorta ¹⁸F-FAPI SUV, and myocardial/liver ¹⁸F-FAPI SUV. One year after treatment, the incidence of MACEs was slightly higher in the group with high ¹⁸F-FAPI myocardial uptake compared to the low uptake group (19.5% vs. 14.0%, log-rank p = 0.621), and the overall survival rate was lower in the high uptake group compared to the low uptake group (57.9% vs. 65.8%, log-rank p = 0.503).</p><p><strong>Conclusions: </strong>The myocardial uptake parameters of ¹⁸F-FDG and ¹⁸F-FAPI PET/CT are helpful in evaluating sorafenib-induced cardiac dysfunction in HCC patients. The level of ¹⁸F-FAPI myocardial uptake has potential value in predicting post-treatment cardiotoxicity and overall survival prognosis in HCC patients.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"324-332"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiogenomic Profiling for Survival Analysis in Gastric Cancer: Integrating CT Imaging, Gene Expression, and Clinical Data.","authors":"Anju R Nath, Kiruthika Thenmozhi, Jeyakumar Natarajan","doi":"10.1007/s11307-025-02019-y","DOIUrl":"10.1007/s11307-025-02019-y","url":null,"abstract":"<p><strong>Purpose: </strong>This study aims to integrate CT (Computed Tomography) radiomic features, gene expression profiles, and clinical data to identify radiogenomic biomarkers and improve overall survival prediction in gastric cancer (GC) patients.</p><p><strong>Procedures: </strong>Quantitative radiomic analysis was performed on 37 GC CT images, alongside gene expression and clinical data, to identify biomarkers associated with overall survival. Tumor segmentation and radiomic feature extraction were followed by Pearson correlation for feature selection. Gene Set Enrichment Analysis (GSEA) identified pathways linking gene expression changes with radiomic features. Regression models were applied to explore the relationships between these pathways, radiomic features, and clinical data in survival prediction.</p><p><strong>Results: </strong>A total of 107 radiomic features were extracted, with 46 radiomic features, 1,032 genes, and one clinical feature (age) selected for further analysis. GSEA identified 29 significant KEGG pathways, mainly involving immune, signal transduction, and catabolism pathways. In survival analysis, the SVM model performed best, identifying age, genes CSF1R and CXCL12, and image features ShortRunHighGrayLevelEmphasis and Idn (Inverse Difference Normalized) as independent predictors.</p><p><strong>Conclusion: </strong>This study highlights the potential of integrating imaging, genomics, and clinical data for prognosis in GC patients, with identified genes suggesting new radiogenomic biomarker candidates for future evaluation.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"353-364"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Analysis of ¹⁸F-FDG PET Radiomics and mRNA Expression in Recurrent/Metastatic Oral Squamous Cell Carcinoma: A Cross-Sectional Study.","authors":"Mai Kim, Wenchao Gu, Reika Kawabata- Iwakawa, Shinichiro Kina, Takahito Nakajima, Tetsuya Higuchi, Masaru Ogawa, Keisuke Suzuki, Yoshito Tsushima, Satoshi Yokoo","doi":"10.1007/s11307-025-02012-5","DOIUrl":"10.1007/s11307-025-02012-5","url":null,"abstract":"<p><strong>Background: </strong>This study explored the relationship between mRNA expression profiles obtained through next-generation sequencing (NGS) and ¹⁸F-fluorodeoxyglucose positron emission tomography (¹⁸F-FDG PET) texture analysis in patients with treatment-resistant oral squamous cell carcinoma (OSCC) who were treated with molecular-targeted drugs. We analyzed the correlation between ¹⁸F-FDG PET texture features and NGS data in a small cohort of five patients with recurrent or metastatic OSCC who received molecular-targeted drugs after surgery. Patients were categorized into two groups based on treatment response: responders (n = 3) and non-responders (n = 2). To validate our findings, we examined transcriptomic data from 292 OSCC patients in The Cancer Genome Atlas (TCGA) database.</p><p><strong>Results: </strong>The gene ankyrin repeat and SOCS box containing two (ASB2) was significantly overexpressed in non-responders and strongly correlated with specific PET radiomic features, including GLRLM_GLNU, GLRLM_RLNU, and GLZLM_GLNU (p < 0.05). High ASB2 expression was also associated with poor prognosis in OSCC patients (p < 0.05) and decreased overall survival, as shown by Kaplan-Meier analysis of the TCGA database (p = 0.017).</p><p><strong>Conclusions: </strong>Integrating ASB2 expression data with ¹⁸F-FDG PET texture features could potentially improve the prediction of treatment outcomes in treatment-resistant OSCC patients undergoing molecular-targeted therapy.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"421-430"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of ¹⁸F-Labeled Deuterated Tropane Derivatives with High Metabolic Stability for PET Imaging of the Dopamine Transporter.","authors":"Jingjing Hong, Jing Kang, Jiaojiao Zuo, Yi Fang, Chunyi Liu, Jingwen Li, Zhengping Chen","doi":"10.1007/s11307-025-02018-z","DOIUrl":"10.1007/s11307-025-02018-z","url":null,"abstract":"<p><strong>Purpose: </strong>Dopamine transporter (DAT) in the central nervous system is an attractive biomarker for the diagnosis and study of various neurodegenerative diseases. To develop in vivo metabolically stable positron emission tomography (PET) probes for DAT imaging with a high target/background ratio, two ¹⁸F-labeled tropane derivatives with deuteration on both the N-fluoropropyl and 2β-carbomethoxy groups of the tropane scaffold were synthesized and evaluated.</p><p><strong>Methods: </strong>Radioligands [¹⁸F]6 and [¹⁸F]10 were synthesized from anhydroecgonine and radiolabeled with ¹⁸F through a \"two-step one-pot\" method. Lipophilicity, in vitro binding assay and microPET imaging in rats were performed. [¹⁸F]10 showed a higher standardized uptake value ratio (SUVr) and was selected for further evaluations by in vivo metabolism and biodistribution.</p><p><strong>Results: </strong>The radioligands [¹⁸F]6 and [¹⁸F]10 were obtained in radiochemical purities > 98% and molar activity of about 30 GBq/μmol. [¹⁸F]6 or [¹⁸F]10 demonstrated high specificity and binding affinity to DAT in vitro, with IC₅₀ values between 2 ~ 3 nM. MicroPET imaging in wild type Sprague-Dawley rats revealed that [¹⁸F]10 has a higher SUVr than [¹⁸F]6. Blocking experiments demonstrated the selectivity and reversibility of [¹⁸F]10 for DAT binding in microPET imaging. The diagnostic efficacy of [¹⁸F]10 for DAT-related disorders was verified in semi-PD model rats with microPET. In vivo metabolic studies in rats indicated that [¹⁸F]10 exhibited enhanced stability. Biodistribution experiments further confirmed that [¹⁸F]10 accumulated in the DAT-rich region of the striatum.</p><p><strong>Conclusion: </strong>[¹⁸F]10 is a highly promising metabolically stable ¹⁸F-labeled PET probe for DAT imaging, with potential clinical applications in detecting and monitoring DAT-related neurological disorders.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"431-441"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a CT-less SPECT Acquisition Protocol for Kidney Dosimetry in ¹⁷⁷Lu-PSMA-617 Radioligand Therapy.","authors":"Christian Happel, Larissa Völler, Benjamin Bockisch, Daniel Groener, Britta Leonhäuser, Frank Grünwald, Amir Sabet","doi":"10.1007/s11307-025-01998-2","DOIUrl":"10.1007/s11307-025-01998-2","url":null,"abstract":"<p><strong>Purpose: </strong>Targeted radioligand therapy of metastatic castration-resistant prostate cancer (mCRPC) with ¹⁷⁷Lu-PSMA (RLT) requires sufficient dose monitoring of the kidneys. Currently, dosimetry using SPECT/CT-imaging is the most preferred method. However, SPECT/CT is a time-consuming procedure and comprises additional radiation exposure to the patient. Moreover, not every therapeutic nuclear medicine facility has access to SPECT/CT. Therefore, the aim of this study was to develop a new procedure of kidney dosimetry without the use of SPECT/CT and evaluate this method in a large cohort of patients with mCRPC undergoing RLT.</p><p><strong>Procedures: </strong>A dedicated torso phantom with kidneys filled with a solution of ¹⁷⁷Lu-PSMA was used for quantitative calibration of a SPECT-camera. The calculated sensitivity was adapted according to the individual attenuation of the patient in four directions from the kidney surface to the body surface (ventral, dorsal, left and right) obtained from a previously performed CT. A total of 196 patients undergoing 926 cycles of ¹⁷⁷Lu-PSMA therapy were retrospectively analyzed. Abdominal SPECT was performed 24, 48 and 72 h after administration of ¹⁷⁷Lu-PSMA including scatter and dead-time correction in every patient. Kidney dose was calculated using an individual attenuation-based procedure and compared to values from international literature.</p><p><strong>Results: </strong>Volumes of interest of the kidneys were drawn in the three sequential SPECT-images to calculate intra-renal effective half-life. Absolute quantification of activity in the kidneys was accomplished obtaining a patient individual sensitivity based on the individual attenuation in the patient. Kidney dose was then calculated applying a bi-exponential time activity curve in Microsoft EXCEL. Mean kidney dose per administered activity was 0.54 (± 0.26) Gy/GBq.</p><p><strong>Conclusions: </strong>With the presented procedure a reliable kidney dosimetry is possible without the use of SPECT/CT. Facilities without SPECT/CT are therefore able to perform an adequate kidney dosimetry without additional radiation exposure for the patient.</p>","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":"400-409"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}